Sec31a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec31a
Entrez Gene ID	69162
Full Name	Sec31 homolog A (S. cerevisiae)
Synonyms	1810024J13Rik,ABP125,ABP130,AU042160,HSPC275,Sec31l1
General protein information	Preferred Names Sec31 homolog A (S. cerevisiae) Names protein transport protein Sec31A SEC31-like 1 SEC31-like protein 1 SEC31-related protein A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 E4

mRNA and Protein(s)

mRNA	Protein	Name
XM_011249559.2	XP_011247861.1	protein transport protein Sec31A isoform X10
XM_011249555.3	XP_011247857.1	protein transport protein Sec31A isoform X5
XM_006535207.4	XP_006535270.1	protein transport protein Sec31A isoform X12
NM_001359350.1	NP_001346279.1	protein transport protein Sec31A isoform c
XM_006535192.2	XP_006535255.1	protein transport protein Sec31A isoform X1
XM_011249553.2	XP_011247855.1	protein transport protein Sec31A isoform X4
XM_011249560.2	XP_011247862.1	protein transport protein Sec31A isoform X11
XM_017321088.1	XP_017176577.1	protein transport protein Sec31A isoform X7
XM_011249557.2	XP_011247859.1	protein transport protein Sec31A isoform X8
XM_017321089.1	XP_017176578.1	protein transport protein Sec31A isoform X8
NM_001359348.1	NP_001346277.1	protein transport protein Sec31A isoform d
NM_001359349.1	NP_001346278.1	protein transport protein Sec31A isoform e
XM_011249555.2	XP_011247857.1	protein transport protein Sec31A isoform X6
XM_011249562.2	XP_011247864.1	protein transport protein Sec31A isoform X13
XM_011249563.2	XP_011247865.1	protein transport protein Sec31A isoform X14
NM_001359344.1	NP_001346273.1	protein transport protein Sec31A isoform d
NM_001359332.1	NP_001346261.1	protein transport protein Sec31A isoform c
XM_006535193.2	XP_006535256.1	protein transport protein Sec31A isoform X1
XM_011249558.2	XP_011247860.1	protein transport protein Sec31A isoform X6
XM_011249559.3	XP_011247861.1	protein transport protein Sec31A isoform X7
XM_011249562.3	XP_011247864.1	protein transport protein Sec31A isoform X10
XM_011249556.2	XP_011247858.1	protein transport protein Sec31A isoform X7
XM_006535193.1	XP_006535256.1	protein transport protein Sec31A isoform X1
XM_011249552.2	XP_011247854.1	protein transport protein Sec31A isoform X3
XM_011249561.2	XP_011247863.1	protein transport protein Sec31A isoform X12
XM_006535207.3	XP_006535270.1	protein transport protein Sec31A isoform X15
XM_006535192.3	XP_006535255.1	protein transport protein Sec31A isoform X1
XM_006535194.2	XP_006535257.1	protein transport protein Sec31A isoform X1
XM_011249560.3	XP_011247862.1	protein transport protein Sec31A isoform X8
XM_011249563.3	XP_011247865.1	protein transport protein Sec31A isoform X11
XM_011249551.2	XP_011247853.1	protein transport protein Sec31A isoform X2
XM_011249554.2	XP_011247856.1	protein transport protein Sec31A isoform X5
NM_026969.1	NP_081245.1	protein transport protein Sec31A isoform b
NM_001359323.1	NP_001346252.1	protein transport protein Sec31A isoform a
XM_011249551.3	XP_011247853.1	protein transport protein Sec31A isoform X2
XM_011249552.3	XP_011247854.1	protein transport protein Sec31A isoform X3
XM_011249553.3	XP_011247855.1	protein transport protein Sec31A isoform X4
XM_011249561.3	XP_011247863.1	protein transport protein Sec31A isoform X9

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sec31a	NP_955824.1
Canis lupus familiaris (dog)	SEC31A	XP_005639183.1
Mus musculus (house mouse)	Sec31a	NP_081245.1
Gallus gallus (chicken)	SEC31A	NP_001026312.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011551	XP_309957.4
Rattus norvegicus (Norway rat)	Sec31a	NP_148981.1
Homo sapiens (human)	SEC31A	NP_001070676.1
Drosophila melanogaster (fruit fly)	sec31	NP_724716.1
Pan troglodytes (chimpanzee)	SEC31A	XP_001140439.1
	SEC31A	XP_001085944.1
Bos taurus (cattle)	SEC31A	NP_001178203.2
Xenopus tropicalis (tropical clawed frog)	sec31a	NP_001011158.2

Gene Ontology
Bibliography

Related articles in PubMed

FGF signalling regulates bone growth through autophagy.
Cinque L, Forrester A, Bartolomeo R, Svelto M, Venditti R, Montefusco S, Polishchuk E, Nusco E, Rossi A, Medina DL, Polishchuk R, De Matteis MA, Settembre C
Nature528(7581)272-5(2015 Dec)

Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to allow ER-Golgi export.
Cho HJ, Yu J, Xie C, Rudrabhatla P, Chen X, Wu J, Parisiadou L, Liu G, Sun L, Ma B, Ding J, Liu Z, Cai H
The EMBO journal33(20)2314-31(2014 Oct)

Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans.
Liegel RP, Handley MT, Ronchetti A, Brown S, Langemeyer L, Linford A, Chang B, Morris-Rosendahl DJ, Carpanini S, Posmyk R, Harthill V, Sheridan E, Abdel-Salam GM, Terhal PA, Faravelli F, Accorsi P, Giordano L, Pinelli L, Hartmann B, Ebert AD, Barr FA, Aligianis IA, Sidjanin DJ
American journal of human genetics93(6)1001-14(2013 Dec)

The E3-ubiquitin ligase TRIM50 interacts with HDAC6 and p62, and promotes the sequestration and clearance of ubiquitinated proteins into the aggresome.
Fusco C, Micale L, Egorov M, Monti M, D'Addetta EV, Augello B, Cozzolino F, Calcagn? A, Fontana A, Polishchuk RS, Didelot G, Reymond A, Pucci P, Merla G
PloS one7(7)e40440(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sec31a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec31a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83591	NM_001359349.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu35661	XM_006535207.4 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu35648	XM_006535192.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu35648	XM_006535193.2 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu70383	XM_011249551.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu70384	XM_011249552.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu70385	XM_011249553.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu70387	XM_011249555.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu70391	XM_011249559.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu70392	XM_011249560.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu70393	XM_011249561.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu70394	XM_011249562.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu70395	XM_011249563.3 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu17676	NM_026969.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70386	NM_001359323.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70388	NM_001359348.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70388	NM_001359344.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70389	NM_001359332.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70389	NM_001359350.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu35648	XM_006535194.2 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70386	XM_011249554.2 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70388	XM_011249556.2 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70388	XM_017321088.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70389	XM_011249557.2 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70389	XM_017321089.1 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70390	XM_011249558.2 Latest version!	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu70387	XM_011249555.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70391	XM_011249559.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70394	XM_011249562.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu35648	XM_006535193.1	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu35661	XM_006535207.3	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu35648	XM_006535192.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70392	XM_011249560.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu70395	XM_011249563.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu70383	XM_011249551.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70384	XM_011249552.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70385	XM_011249553.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu70393	XM_011249561.2	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec31a ( XM_006535207.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535270.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X19, mRNA.

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CloneID	OMu35661
Clone ID Related Accession (Same CDS sequence)	XM_006535207.4 , XM_006535207.3
Accession Version	XM_006535207.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTCCTGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535270.1
CDS	155..2908
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSSCF

Target ORF information:

RefSeq Version	XM_006535207.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535207.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTCCTGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( NM_001359349.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346278.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 6, mRNA.

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CloneID	OMu83591
Clone ID Related Accession (Same CDS sequence)	NM_001359349.1
Accession Version	NM_001359349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107744.8. Transcript Variant: This variant (6), differs in the 5' UTR and lacks two alternate exons compared to variant 1. The resulting isoform (e) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.7562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATC CTGAGTTGTT GTTAAGCTGT GGAAAAGATG CTAAGATTCT CTGCTCTAAC 
CCTAACACAG GAGAGGTATT ATATGAACTT CCCACCAACA CACAGTGGTG CTTTGATATC 
CAGTGGTGTC CTCGAAATCC TGCTGTCCTG TCCGCTGCTT CCTTTGATGG ACGGATCAGT 
GTCTATTCCA TCATGGGAGG GAGCATAGAC GGCTTACGGC AGAAACAAGT TGACAAGCTT 
TCATCATCTT TTGGGAATCT TGATCCTTTC GGCACAGGAC AGCCTCTCCC TCCATTACAA 
ATCCCACAGC AGAGCGCTCA GCACAGCATA GTGCTGCCCC TGAAGAAGCC TCCCAAGTGG 
ATCCGGAGGC CTGTGGGCGC TTCCTTCTCG TTTGGAGGGA AGTTGGTGAC TTTTGAGAGC 
GTTGCAGTGC CGCTGCAGCA GGGAGCTGAG CAGCAGCGAC GGCAGCCTGT GTTCATCAGT 
CAGGTGGTGA CGGAGAAGGA CTTTCTCAAC CGGTCGGCGC AGCTGCAGCA TGCTGTGCAG 
TCTCAGGGCT TCATCGGTTA CTGCCAGAAA AAGATCGAGG CTTCCCAGAC CGAGTTTGAG 
AAAAATGTGT GGTCCTTCTT GAAGGTAAAC TTTGAGGAGG ATTCTCGTGG AAAATACCTT 
GAGCTTCTGG GATACAGAAA GGAAGATCTG GGGCAGAAGA TTGCTTTGGC CTTGAACAAA 
GTGGACGGAC CCGATGTGGC TCTTAAGGAC TCTGACCAAG TAGCGCAGAG TGATGGGGAG 
GAGAGTCCTG CTGCTGAAGA GCAGCTCTTG GGAGAGCGCA TTAAAGAGGA AAAACAGGAA 
TGTGACTTTC TGCCCTCGGC GGGAGGGACG TTTAACATCT CTGTCAGTGG GGATATTGAC 
GGTCTAATTA CCCGGGCCTT GCTGACAGGC AATTTTGAGA GTGCAGTTGA CCTTTGCTTA 
CATGATAACC GAATGGCTGA TGCCATTATA TTAGCCATAG CAGGTGGACA GGAACTCTTG 
GCTCAAACAC AAAAGAAATA CTTCGCAAAA TCCCAAAGCA AAATTACCAG GCTTATCACT 
GCAGTGGTGA TGAAGAACTG GAGGGAGATT GTTGAATCCT GTGATCTTAA GAATTGGAGA 
GAGGCTTTAG CTGCAGTCCT CACTTACGCT AAACCAGACG AGTTCTCGGC CCTGTGCGAT 
CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA GACAGCCTCC TGCGGACTCA GGCCTGCCTC 
TGCTACATCT GTGCAGGGAA TGTAGAGAGA TTGGTTGCTT GCTGGACAAA AGCTCAGGAC 
GGCAGCAGCC CCTTGTCTCT CCAGGATCTG ATTGAGAAAG TTGTCATCCT CCGCAAAGCT 
GTACAGCTCA CCCAAGCCTT GGACACCAAT ACTGTCGGGG CTCTTCTGGC TGAGAAGATG 
AGTCAGTATG CCAGCTTGTT GGCAGCCCAG GGCAGCATTG CCGCAGCCTT GGCTTTTCTG 
CCTGACAACA CCAACCAGCC AAATATTGTG CAGCTGCGTG ACAGACTCTG TAAAGCTCAA 
GGCAAGCCTG TGTCGGGACA GGAATCTTCT CAGAGCCCAT ATGAGAGGCA GCCACTGTCC 
AAGGGCAGGC CTGGACCAGT TGCTGGCCAC TCGCAGATGC CCAGAGTTCA AACTCAGCAA 
TATTATCCCC ATGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTC CTCAGAATGG TTGGAATGAT CCTCCAGCTC TGAACAGAGT GCCGAAGAAG 
AAGAAGATGC CTGAAAACTT CATGCCTCCT GTTCCCATTA CGTCACCAAT CATGAACCCT 
TCGGGTGATC CCCAGTCACA GGGGCTGCAG CAGCAGCCTT CAACTCCAGG GCCGCTGTCA 
AGCCACGCCT CCTTCCCACA GCAGCACCTA GCGGGCGGCC AGCCCTTCCA TGGTGTACAG 
CAGCCTCTTG CTCAGACAGG CATGCCGCCG TCCTTCTCAA AGCCCAACAC TGAAGGGGCT 
CCAGGAGCTC CCATTGGAAA CACCATCCAG CACGTGCAGG CTCTGCCAAC AGAAAAGATT 
ACTAAGAAAC CTATTCCGGA GGAGCACCTC ATCCTAAAGA CGACATTTGA GGACCTTATC 
CAGCGCTGCC TCTCCTCCGC TACAGACCCC CAAACCAAGA GGAAGCTGGA CGACGCCAGC 
AAGCGCCTGG AGTTCCTCTA CGACAAGCTT AGGGAACAGA CACTCTCCCC GACCATCATC 
AACGGTCTCC ACAGCATCGC CAGGAGCATT GAGACGAGGA ACTACTCGGA AGGGTTGAGC 
GTCCACACTC ACATCGTCAG CACCAGCAAC TTCAGTGAGA CCTCGGCCTT CATGCCCGTC 
CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346278.1
CDS	812..3613
Translation	MADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	NM_001359349.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359349.1

ORF Insert Sequence:

ATGGCAGATC CTGAGTTGTT GTTAAGCTGT GGAAAAGATG CTAAGATTCT CTGCTCTAAC 
CCTAACACAG GAGAGGTATT ATATGAACTT CCCACCAACA CACAGTGGTG CTTTGATATC 
CAGTGGTGTC CTCGAAATCC TGCTGTCCTG TCCGCTGCTT CCTTTGATGG ACGGATCAGT 
GTCTATTCCA TCATGGGAGG GAGCATAGAC GGCTTACGGC AGAAACAAGT TGACAAGCTT 
TCATCATCTT TTGGGAATCT TGATCCTTTC GGCACAGGAC AGCCTCTCCC TCCATTACAA 
ATCCCACAGC AGAGCGCTCA GCACAGCATA GTGCTGCCCC TGAAGAAGCC TCCCAAGTGG 
ATCCGGAGGC CTGTGGGCGC TTCCTTCTCG TTTGGAGGGA AGTTGGTGAC TTTTGAGAGC 
GTTGCAGTGC CGCTGCAGCA GGGAGCTGAG CAGCAGCGAC GGCAGCCTGT GTTCATCAGT 
CAGGTGGTGA CGGAGAAGGA CTTTCTCAAC CGGTCGGCGC AGCTGCAGCA TGCTGTGCAG 
TCTCAGGGCT TCATCGGTTA CTGCCAGAAA AAGATCGAGG CTTCCCAGAC CGAGTTTGAG 
AAAAATGTGT GGTCCTTCTT GAAGGTAAAC TTTGAGGAGG ATTCTCGTGG AAAATACCTT 
GAGCTTCTGG GATACAGAAA GGAAGATCTG GGGCAGAAGA TTGCTTTGGC CTTGAACAAA 
GTGGACGGAC CCGATGTGGC TCTTAAGGAC TCTGACCAAG TAGCGCAGAG TGATGGGGAG 
GAGAGTCCTG CTGCTGAAGA GCAGCTCTTG GGAGAGCGCA TTAAAGAGGA AAAACAGGAA 
TGTGACTTTC TGCCCTCGGC GGGAGGGACG TTTAACATCT CTGTCAGTGG GGATATTGAC 
GGTCTAATTA CCCGGGCCTT GCTGACAGGC AATTTTGAGA GTGCAGTTGA CCTTTGCTTA 
CATGATAACC GAATGGCTGA TGCCATTATA TTAGCCATAG CAGGTGGACA GGAACTCTTG 
GCTCAAACAC AAAAGAAATA CTTCGCAAAA TCCCAAAGCA AAATTACCAG GCTTATCACT 
GCAGTGGTGA TGAAGAACTG GAGGGAGATT GTTGAATCCT GTGATCTTAA GAATTGGAGA 
GAGGCTTTAG CTGCAGTCCT CACTTACGCT AAACCAGACG AGTTCTCGGC CCTGTGCGAT 
CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA GACAGCCTCC TGCGGACTCA GGCCTGCCTC 
TGCTACATCT GTGCAGGGAA TGTAGAGAGA TTGGTTGCTT GCTGGACAAA AGCTCAGGAC 
GGCAGCAGCC CCTTGTCTCT CCAGGATCTG ATTGAGAAAG TTGTCATCCT CCGCAAAGCT 
GTACAGCTCA CCCAAGCCTT GGACACCAAT ACTGTCGGGG CTCTTCTGGC TGAGAAGATG 
AGTCAGTATG CCAGCTTGTT GGCAGCCCAG GGCAGCATTG CCGCAGCCTT GGCTTTTCTG 
CCTGACAACA CCAACCAGCC AAATATTGTG CAGCTGCGTG ACAGACTCTG TAAAGCTCAA 
GGCAAGCCTG TGTCGGGACA GGAATCTTCT CAGAGCCCAT ATGAGAGGCA GCCACTGTCC 
AAGGGCAGGC CTGGACCAGT TGCTGGCCAC TCGCAGATGC CCAGAGTTCA AACTCAGCAA 
TATTATCCCC ATGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTC CTCAGAATGG TTGGAATGAT CCTCCAGCTC TGAACAGAGT GCCGAAGAAG 
AAGAAGATGC CTGAAAACTT CATGCCTCCT GTTCCCATTA CGTCACCAAT CATGAACCCT 
TCGGGTGATC CCCAGTCACA GGGGCTGCAG CAGCAGCCTT CAACTCCAGG GCCGCTGTCA 
AGCCACGCCT CCTTCCCACA GCAGCACCTA GCGGGCGGCC AGCCCTTCCA TGGTGTACAG 
CAGCCTCTTG CTCAGACAGG CATGCCGCCG TCCTTCTCAA AGCCCAACAC TGAAGGGGCT 
CCAGGAGCTC CCATTGGAAA CACCATCCAG CACGTGCAGG CTCTGCCAAC AGAAAAGATT 
ACTAAGAAAC CTATTCCGGA GGAGCACCTC ATCCTAAAGA CGACATTTGA GGACCTTATC 
CAGCGCTGCC TCTCCTCCGC TACAGACCCC CAAACCAAGA GGAAGCTGGA CGACGCCAGC 
AAGCGCCTGG AGTTCCTCTA CGACAAGCTT AGGGAACAGA CACTCTCCCC GACCATCATC 
AACGGTCTCC ACAGCATCGC CAGGAGCATT GAGACGAGGA ACTACTCGGA AGGGTTGAGC 
GTCCACACTC ACATCGTCAG CACCAGCAAC TTCAGTGAGA CCTCGGCCTT CATGCCCGTC 
CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_006535207.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535270.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X14, mRNA.

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CloneID	OMu35661
Clone ID Related Accession (Same CDS sequence)	XM_006535207.4 , XM_006535207.3
Accession Version	XM_006535207.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535207.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTCCTGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535270.1
CDS	173..2926
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSSCF

Target ORF information:

RefSeq Version	XM_006535207.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535207.4

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTCCTGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_006535193.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535256.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu35648
Clone ID Related Accession (Same CDS sequence)	XM_006535192.2 , XM_006535193.2 , XM_006535193.1 , XM_006535192.3 , XM_006535194.2
Accession Version	XM_006535193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535256.1
CDS	110..3895
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_006535193.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535193.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_006535192.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535255.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35648
Clone ID Related Accession (Same CDS sequence)	XM_006535192.2 , XM_006535193.2 , XM_006535193.1 , XM_006535192.3 , XM_006535194.2
Accession Version	XM_006535192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535255.1
CDS	173..3958
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_006535192.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535192.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249551.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247853.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X4, mRNA.

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CloneID	OMu70383
Clone ID Related Accession (Same CDS sequence)	XM_011249551.2 , XM_011249551.3
Accession Version	XM_011249551.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249551.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG 
AATGGTTGGA ATGATCCTCC AGCTCTGAAC AGAGTGCCGA AGAAGAAGAA GATGCCTGAA 
AACTTCATGC CTCCTGTTCC CATTACGTCA CCAATCATGA ACCCTTCGGG TGATCCCCAG 
TCACAGGGGC TGCAGCAGCA GCCTTCAACT CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC 
CCACAGCAGC ACCTAGCGGG CGGCCAGCCC TTCCATGGTG TACAGCAGCC TCTTGCTCAG 
ACAGGCATGC CGCCGTCCTT CTCAAAGCCC AACACTGAAG GGGCTCCAGG AGCTCCCATT 
GGAAACACCA TCCAGCACGT GCAGGCTCTG CCAACAGAAA AGATTACTAA GAAACCTATT 
CCGGAGGAGC ACCTCATCCT AAAGACGACA TTTGAGGACC TTATCCAGCG CTGCCTCTCC 
TCCGCTACAG ACCCCCAAAC CAAGAGGAAG CTGGACGACG CCAGCAAGCG CCTGGAGTTC 
CTCTACGACA AGCTTAGGGA ACAGACACTC TCCCCGACCA TCATCAACGG TCTCCACAGC 
ATCGCCAGGA GCATTGAGAC GAGGAACTAC TCGGAAGGGT TGAGCGTCCA CACTCACATC 
GTCAGCACCA GCAACTTCAG TGAGACCTCG GCCTTCATGC CCGTCCTCAA GGTTGTCCTC 
AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247853.1
CDS	173..3919
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249551.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249551.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG 
AATGGTTGGA ATGATCCTCC AGCTCTGAAC AGAGTGCCGA AGAAGAAGAA GATGCCTGAA 
AACTTCATGC CTCCTGTTCC CATTACGTCA CCAATCATGA ACCCTTCGGG TGATCCCCAG 
TCACAGGGGC TGCAGCAGCA GCCTTCAACT CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC 
CCACAGCAGC ACCTAGCGGG CGGCCAGCCC TTCCATGGTG TACAGCAGCC TCTTGCTCAG 
ACAGGCATGC CGCCGTCCTT CTCAAAGCCC AACACTGAAG GGGCTCCAGG AGCTCCCATT 
GGAAACACCA TCCAGCACGT GCAGGCTCTG CCAACAGAAA AGATTACTAA GAAACCTATT 
CCGGAGGAGC ACCTCATCCT AAAGACGACA TTTGAGGACC TTATCCAGCG CTGCCTCTCC 
TCCGCTACAG ACCCCCAAAC CAAGAGGAAG CTGGACGACG CCAGCAAGCG CCTGGAGTTC 
CTCTACGACA AGCTTAGGGA ACAGACACTC TCCCCGACCA TCATCAACGG TCTCCACAGC 
ATCGCCAGGA GCATTGAGAC GAGGAACTAC TCGGAAGGGT TGAGCGTCCA CACTCACATC 
GTCAGCACCA GCAACTTCAG TGAGACCTCG GCCTTCATGC CCGTCCTCAA GGTTGTCCTC 
AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249552.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247854.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu70384
Clone ID Related Accession (Same CDS sequence)	XM_011249552.2 , XM_011249552.3
Accession Version	XM_011249552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGAAA ATCAATCTTT CCAAGACCAG GCATCTATAT TGGAAGGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247854.1
CDS	173..3913
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249552.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249552.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGAAA ATCAATCTTT CCAAGACCAG GCATCTATAT TGGAAGGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249553.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247855.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu70385
Clone ID Related Accession (Same CDS sequence)	XM_011249553.2 , XM_011249553.3
Accession Version	XM_011249553.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGCGCAT TAAAGAGGAA 
AAACAGGAAT GTGACTTTCT GCCCTCGGCG GGAGGGACGT TTAACATCTC TGTCAGTGGG 
GATATTGACG GTCTAATTAC CCGGGCCTTG CTGACAGGCA ATTTTGAGAG TGCAGTTGAC 
CTTTGCTTAC ATGATAACCG AATGGCTGAT GCCATTATAT TAGCCATAGC AGGTGGACAG 
GAACTCTTGG CTCAAACACA AAAGAAATAC TTCGCAAAAT CCCAAAGCAA AATTACCAGG 
CTTATCACTG CAGTGGTGAT GAAGAACTGG AGGGAGATTG TTGAATCCTG TGATCTTAAG 
AATTGGAGAG AGGCTTTAGC TGCAGTCCTC ACTTACGCTA AACCAGACGA GTTCTCGGCC 
CTGTGCGATC TCCTGGGAAC CCGTCTGGAA AGAGAAGGAG ACAGCCTCCT GCGGACTCAG 
GCCTGCCTCT GCTACATCTG TGCAGGGAAT GTAGAGAGAT TGGTTGCTTG CTGGACAAAA 
GCTCAGGACG GCAGCAGCCC CTTGTCTCTC CAGGATCTGA TTGAGAAAGT TGTCATCCTC 
CGCAAAGCTG TACAGCTCAC CCAAGCCTTG GACACCAATA CTGTCGGGGC TCTTCTGGCT 
GAGAAGATGA GTCAGTATGC CAGCTTGTTG GCAGCCCAGG GCAGCATTGC CGCAGCCTTG 
GCTTTTCTGC CTGACAACAC CAACCAGCCA AATATTGTGC AGCTGCGTGA CAGACTCTGT 
AAAGCTCAAG GCAAGCCTGT GTCGGGACAG GAATCTTCTC AGAGCCCATA TGAGAGGCAG 
CCACTGTCCA AGGGCAGGCC TGGACCAGTT GCTGGCCACT CGCAGATGCC CAGAGTTCAA 
ACTCAGCAAT ATTATCCCCA TGTTAGAATT GCCCCTACTG TCACTACCTG GAGTAACAAA 
ACTCCCACTG CCCTCCCCAG CCATCCACCT GCAGCCTCTC CCTCTGACAC ACAGGGAGAA 
AATCCCCCAC CTCCAGGTTT CATAATGCAG GGCAATGTCA TTCCAAACCC TGCTGCCCCG 
CTGCCCACGG CTCCAGGCCA CATGCCCAGC CAGCTGCCAC CTTACCCACA GCCGCAGCCT 
TACCAGCCAG CCCAGCAGTA TTCCTTCGGA ACAGGGGGGG CAGCAGCTTA TCGACCTCAA 
CAGCCTGTTG CTCCTCCTGC TTCTAACGCT TACCCTAACA CCCCGTACAT ATCTCCTGTG 
GCTTCCTATT CTGGGCAGCC TCAGATGTAC ACAGCCCAGC AAGCCTCCTC ACCTACCTCC 
AGCTCTGCTG CTTCTTTCCC CCCTCCCTCC TCTGGAGCAT CCTTCCAGCA TGGCGGACCA 
GGAGCTCCAC CATCATCTTC AGCTTATGCA CTGCCTCCTG GAACAACAGG TACACCGCCT 
GCTGCCAGTG AGCTGCCTGC GTCCCAAAGA ACAGAAAATC AATCTTTCCA AGACCAGGCA 
TCTATATTGG AAGGTCCTCA GAATGGTTGG AATGATCCTC CAGCTCTGAA CAGAGTGCCG 
AAGAAGAAGA AGATGCCTGA AAACTTCATG CCTCCTGTTC CCATTACGTC ACCAATCATG 
AACCCTTCGG GTGATCCCCA GTCACAGGGG CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG 
CTGTCAAGCC ACGCCTCCTT CCCACAGCAG CACCTAGCGG GCGGCCAGCC CTTCCATGGT 
GTACAGCAGC CTCTTGCTCA GACAGGCATG CCGCCGTCCT TCTCAAAGCC CAACACTGAA 
GGGGCTCCAG GAGCTCCCAT TGGAAACACC ATCCAGCACG TGCAGGCTCT GCCAACAGAA 
AAGATTACTA AGAAACCTAT TCCGGAGGAG CACCTCATCC TAAAGACGAC ATTTGAGGAC 
CTTATCCAGC GCTGCCTCTC CTCCGCTACA GACCCCCAAA CCAAGAGGAA GCTGGACGAC 
GCCAGCAAGC GCCTGGAGTT CCTCTACGAC AAGCTTAGGG AACAGACACT CTCCCCGACC 
ATCATCAACG GTCTCCACAG CATCGCCAGG AGCATTGAGA CGAGGAACTA CTCGGAAGGG 
TTGAGCGTCC ACACTCACAT CGTCAGCACC AGCAACTTCA GTGAGACCTC GGCCTTCATG 
CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247855.1
CDS	150..3857
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249553.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249553.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGCGCAT TAAAGAGGAA 
AAACAGGAAT GTGACTTTCT GCCCTCGGCG GGAGGGACGT TTAACATCTC TGTCAGTGGG 
GATATTGACG GTCTAATTAC CCGGGCCTTG CTGACAGGCA ATTTTGAGAG TGCAGTTGAC 
CTTTGCTTAC ATGATAACCG AATGGCTGAT GCCATTATAT TAGCCATAGC AGGTGGACAG 
GAACTCTTGG CTCAAACACA AAAGAAATAC TTCGCAAAAT CCCAAAGCAA AATTACCAGG 
CTTATCACTG CAGTGGTGAT GAAGAACTGG AGGGAGATTG TTGAATCCTG TGATCTTAAG 
AATTGGAGAG AGGCTTTAGC TGCAGTCCTC ACTTACGCTA AACCAGACGA GTTCTCGGCC 
CTGTGCGATC TCCTGGGAAC CCGTCTGGAA AGAGAAGGAG ACAGCCTCCT GCGGACTCAG 
GCCTGCCTCT GCTACATCTG TGCAGGGAAT GTAGAGAGAT TGGTTGCTTG CTGGACAAAA 
GCTCAGGACG GCAGCAGCCC CTTGTCTCTC CAGGATCTGA TTGAGAAAGT TGTCATCCTC 
CGCAAAGCTG TACAGCTCAC CCAAGCCTTG GACACCAATA CTGTCGGGGC TCTTCTGGCT 
GAGAAGATGA GTCAGTATGC CAGCTTGTTG GCAGCCCAGG GCAGCATTGC CGCAGCCTTG 
GCTTTTCTGC CTGACAACAC CAACCAGCCA AATATTGTGC AGCTGCGTGA CAGACTCTGT 
AAAGCTCAAG GCAAGCCTGT GTCGGGACAG GAATCTTCTC AGAGCCCATA TGAGAGGCAG 
CCACTGTCCA AGGGCAGGCC TGGACCAGTT GCTGGCCACT CGCAGATGCC CAGAGTTCAA 
ACTCAGCAAT ATTATCCCCA TGTTAGAATT GCCCCTACTG TCACTACCTG GAGTAACAAA 
ACTCCCACTG CCCTCCCCAG CCATCCACCT GCAGCCTCTC CCTCTGACAC ACAGGGAGAA 
AATCCCCCAC CTCCAGGTTT CATAATGCAG GGCAATGTCA TTCCAAACCC TGCTGCCCCG 
CTGCCCACGG CTCCAGGCCA CATGCCCAGC CAGCTGCCAC CTTACCCACA GCCGCAGCCT 
TACCAGCCAG CCCAGCAGTA TTCCTTCGGA ACAGGGGGGG CAGCAGCTTA TCGACCTCAA 
CAGCCTGTTG CTCCTCCTGC TTCTAACGCT TACCCTAACA CCCCGTACAT ATCTCCTGTG 
GCTTCCTATT CTGGGCAGCC TCAGATGTAC ACAGCCCAGC AAGCCTCCTC ACCTACCTCC 
AGCTCTGCTG CTTCTTTCCC CCCTCCCTCC TCTGGAGCAT CCTTCCAGCA TGGCGGACCA 
GGAGCTCCAC CATCATCTTC AGCTTATGCA CTGCCTCCTG GAACAACAGG TACACCGCCT 
GCTGCCAGTG AGCTGCCTGC GTCCCAAAGA ACAGAAAATC AATCTTTCCA AGACCAGGCA 
TCTATATTGG AAGGTCCTCA GAATGGTTGG AATGATCCTC CAGCTCTGAA CAGAGTGCCG 
AAGAAGAAGA AGATGCCTGA AAACTTCATG CCTCCTGTTC CCATTACGTC ACCAATCATG 
AACCCTTCGG GTGATCCCCA GTCACAGGGG CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG 
CTGTCAAGCC ACGCCTCCTT CCCACAGCAG CACCTAGCGG GCGGCCAGCC CTTCCATGGT 
GTACAGCAGC CTCTTGCTCA GACAGGCATG CCGCCGTCCT TCTCAAAGCC CAACACTGAA 
GGGGCTCCAG GAGCTCCCAT TGGAAACACC ATCCAGCACG TGCAGGCTCT GCCAACAGAA 
AAGATTACTA AGAAACCTAT TCCGGAGGAG CACCTCATCC TAAAGACGAC ATTTGAGGAC 
CTTATCCAGC GCTGCCTCTC CTCCGCTACA GACCCCCAAA CCAAGAGGAA GCTGGACGAC 
GCCAGCAAGC GCCTGGAGTT CCTCTACGAC AAGCTTAGGG AACAGACACT CTCCCCGACC 
ATCATCAACG GTCTCCACAG CATCGCCAGG AGCATTGAGA CGAGGAACTA CTCGGAAGGG 
TTGAGCGTCC ACACTCACAT CGTCAGCACC AGCAACTTCA GTGAGACCTC GGCCTTCATG 
CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249555.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247857.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X7, mRNA.

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CloneID	OMu70387
Clone ID Related Accession (Same CDS sequence)	XM_011249555.3 , XM_011249555.2
Accession Version	XM_011249555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGTTAGAAT TGCCCCTACT 
GTCACTACCT GGAGTAACAA AACTCCCACT GCCCTCCCCA GCCATCCACC TGCAGCCTCT 
CCCTCTGACA CACAGGGAGA AAATCCCCCA CCTCCAGGTT TCATAATGCA GGGCAATGTC 
ATTCCAAACC CTGCTGCCCC GCTGCCCACG GCTCCAGGCC ACATGCCCAG CCAGCTGCCA 
CCTTACCCAC AGCCGCAGCC TTACCAGCCA GCCCAGCAGT ATTCCTTCGG AACAGGGGGG 
GCAGCAGCTT ATCGACCTCA ACAGCCTGTT GCTCCTCCTG CTTCTAACGC TTACCCTAAC 
ACCCCGTACA TATCTCCTGT GGCTTCCTAT TCTGGGCAGC CTCAGATGTA CACAGCCCAG 
CAAGCCTCCT CACCTACCTC CAGCTCTGCT GCTTCTTTCC CCCCTCCCTC CTCTGGAGCA 
TCCTTCCAGC ATGGCGGACC AGGAGCTCCA CCATCATCTT CAGCTTATGC ACTGCCTCCT 
GGAACAACAG GTACACCGCC TGCTGCCAGT GAGCTGCCTG CGTCCCAAAG AACAGAAAAT 
CAATCTTTCC AAGACCAGGC ATCTATATTG GAAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247857.1
CDS	150..3818
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249555.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249555.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGTTAGAAT TGCCCCTACT 
GTCACTACCT GGAGTAACAA AACTCCCACT GCCCTCCCCA GCCATCCACC TGCAGCCTCT 
CCCTCTGACA CACAGGGAGA AAATCCCCCA CCTCCAGGTT TCATAATGCA GGGCAATGTC 
ATTCCAAACC CTGCTGCCCC GCTGCCCACG GCTCCAGGCC ACATGCCCAG CCAGCTGCCA 
CCTTACCCAC AGCCGCAGCC TTACCAGCCA GCCCAGCAGT ATTCCTTCGG AACAGGGGGG 
GCAGCAGCTT ATCGACCTCA ACAGCCTGTT GCTCCTCCTG CTTCTAACGC TTACCCTAAC 
ACCCCGTACA TATCTCCTGT GGCTTCCTAT TCTGGGCAGC CTCAGATGTA CACAGCCCAG 
CAAGCCTCCT CACCTACCTC CAGCTCTGCT GCTTCTTTCC CCCCTCCCTC CTCTGGAGCA 
TCCTTCCAGC ATGGCGGACC AGGAGCTCCA CCATCATCTT CAGCTTATGC ACTGCCTCCT 
GGAACAACAG GTACACCGCC TGCTGCCAGT GAGCTGCCTG CGTCCCAAAG AACAGAAAAT 
CAATCTTTCC AAGACCAGGC ATCTATATTG GAAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249559.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247861.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X9, mRNA.

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CloneID	OMu70391
Clone ID Related Accession (Same CDS sequence)	XM_011249559.2 , XM_011249559.3
Accession Version	XM_011249559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTA CACCGCCTGC TGCCAGTGAG 
CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG AATGGTTGGA ATGATCCTCC AGCTCTGAAC 
AGAGTGCCGA AGAAGAAGAA GATGCCTGAA AACTTCATGC CTCCTGTTCC CATTACGTCA 
CCAATCATGA ACCCTTCGGG TGATCCCCAG TCACAGGGGC TGCAGCAGCA GCCTTCAACT 
CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC CCACAGCAGC ACCTAGCGGG CGGCCAGCCC 
TTCCATGGTG TACAGCAGCC TCTTGCTCAG ACAGGCATGC CGCCGTCCTT CTCAAAGCCC 
AACACTGAAG GGGCTCCAGG AGCTCCCATT GGAAACACCA TCCAGCACGT GCAGGCTCTG 
CCAACAGAAA AGATTACTAA GAAACCTATT CCGGAGGAGC ACCTCATCCT AAAGACGACA 
TTTGAGGACC TTATCCAGCG CTGCCTCTCC TCCGCTACAG ACCCCCAAAC CAAGAGGAAG 
CTGGACGACG CCAGCAAGCG CCTGGAGTTC CTCTACGACA AGCTTAGGGA ACAGACACTC 
TCCCCGACCA TCATCAACGG TCTCCACAGC ATCGCCAGGA GCATTGAGAC GAGGAACTAC 
TCGGAAGGGT TGAGCGTCCA CACTCACATC GTCAGCACCA GCAACTTCAG TGAGACCTCG 
GCCTTCATGC CCGTCCTCAA GGTTGTCCTC AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247861.1
CDS	149..3685
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249559.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249559.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTA CACCGCCTGC TGCCAGTGAG 
CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG AATGGTTGGA ATGATCCTCC AGCTCTGAAC 
AGAGTGCCGA AGAAGAAGAA GATGCCTGAA AACTTCATGC CTCCTGTTCC CATTACGTCA 
CCAATCATGA ACCCTTCGGG TGATCCCCAG TCACAGGGGC TGCAGCAGCA GCCTTCAACT 
CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC CCACAGCAGC ACCTAGCGGG CGGCCAGCCC 
TTCCATGGTG TACAGCAGCC TCTTGCTCAG ACAGGCATGC CGCCGTCCTT CTCAAAGCCC 
AACACTGAAG GGGCTCCAGG AGCTCCCATT GGAAACACCA TCCAGCACGT GCAGGCTCTG 
CCAACAGAAA AGATTACTAA GAAACCTATT CCGGAGGAGC ACCTCATCCT AAAGACGACA 
TTTGAGGACC TTATCCAGCG CTGCCTCTCC TCCGCTACAG ACCCCCAAAC CAAGAGGAAG 
CTGGACGACG CCAGCAAGCG CCTGGAGTTC CTCTACGACA AGCTTAGGGA ACAGACACTC 
TCCCCGACCA TCATCAACGG TCTCCACAGC ATCGCCAGGA GCATTGAGAC GAGGAACTAC 
TCGGAAGGGT TGAGCGTCCA CACTCACATC GTCAGCACCA GCAACTTCAG TGAGACCTCG 
GCCTTCATGC CCGTCCTCAA GGTTGTCCTC AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249560.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247862.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X10, mRNA.

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CloneID	OMu70392
Clone ID Related Accession (Same CDS sequence)	XM_011249560.2 , XM_011249560.3
Accession Version	XM_011249560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTC CTCAGAATGG TTGGAATGAT 
CCTCCAGCTC TGAACAGAGT GCCGAAGAAG AAGAAGATGC CTGAAAACTT CATGCCTCCT 
GTTCCCATTA CGTCACCAAT CATGAACCCT TCGGGTGATC CCCAGTCACA GGGGCTGCAG 
CAGCAGCCTT CAACTCCAGG GCCGCTGTCA AGCCACGCCT CCTTCCCACA GCAGCACCTA 
GCGGGCGGCC AGCCCTTCCA TGGTGTACAG CAGCCTCTTG CTCAGACAGG CATGCCGCCG 
TCCTTCTCAA AGCCCAACAC TGAAGGGGCT CCAGGAGCTC CCATTGGAAA CACCATCCAG 
CACGTGCAGG CTCTGCCAAC AGAAAAGATT ACTAAGAAAC CTATTCCGGA GGAGCACCTC 
ATCCTAAAGA CGACATTTGA GGACCTTATC CAGCGCTGCC TCTCCTCCGC TACAGACCCC 
CAAACCAAGA GGAAGCTGGA CGACGCCAGC AAGCGCCTGG AGTTCCTCTA CGACAAGCTT 
AGGGAACAGA CACTCTCCCC GACCATCATC AACGGTCTCC ACAGCATCGC CAGGAGCATT 
GAGACGAGGA ACTACTCGGA AGGGTTGAGC GTCCACACTC ACATCGTCAG CACCAGCAAC 
TTCAGTGAGA CCTCGGCCTT CATGCCCGTC CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG 
CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247862.1
CDS	149..3640
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249560.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249560.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTC CTCAGAATGG TTGGAATGAT 
CCTCCAGCTC TGAACAGAGT GCCGAAGAAG AAGAAGATGC CTGAAAACTT CATGCCTCCT 
GTTCCCATTA CGTCACCAAT CATGAACCCT TCGGGTGATC CCCAGTCACA GGGGCTGCAG 
CAGCAGCCTT CAACTCCAGG GCCGCTGTCA AGCCACGCCT CCTTCCCACA GCAGCACCTA 
GCGGGCGGCC AGCCCTTCCA TGGTGTACAG CAGCCTCTTG CTCAGACAGG CATGCCGCCG 
TCCTTCTCAA AGCCCAACAC TGAAGGGGCT CCAGGAGCTC CCATTGGAAA CACCATCCAG 
CACGTGCAGG CTCTGCCAAC AGAAAAGATT ACTAAGAAAC CTATTCCGGA GGAGCACCTC 
ATCCTAAAGA CGACATTTGA GGACCTTATC CAGCGCTGCC TCTCCTCCGC TACAGACCCC 
CAAACCAAGA GGAAGCTGGA CGACGCCAGC AAGCGCCTGG AGTTCCTCTA CGACAAGCTT 
AGGGAACAGA CACTCTCCCC GACCATCATC AACGGTCTCC ACAGCATCGC CAGGAGCATT 
GAGACGAGGA ACTACTCGGA AGGGTTGAGC GTCCACACTC ACATCGTCAG CACCAGCAAC 
TTCAGTGAGA CCTCGGCCTT CATGCCCGTC CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG 
CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249561.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247863.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X11, mRNA.

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CloneID	OMu70393
Clone ID Related Accession (Same CDS sequence)	XM_011249561.2 , XM_011249561.3
Accession Version	XM_011249561.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCGTCC TCAGAATGGT TGGAATGATC CTCCAGCTCT GAACAGAGTG 
CCGAAGAAGA AGAAGATGCC TGAAAACTTC ATGCCTCCTG TTCCCATTAC GTCACCAATC 
ATGAACCCTT CGGGTGATCC CCAGTCACAG GGGCTGCAGC AGCAGCCTTC AACTCCAGGG 
CCGCTGTCAA GCCACGCCTC CTTCCCACAG CAGCACCTAG CGGGCGGCCA GCCCTTCCAT 
GGTGTACAGC AGCCTCTTGC TCAGACAGGC ATGCCGCCGT CCTTCTCAAA GCCCAACACT 
GAAGGGGCTC CAGGAGCTCC CATTGGAAAC ACCATCCAGC ACGTGCAGGC TCTGCCAACA 
GAAAAGATTA CTAAGAAACC TATTCCGGAG GAGCACCTCA TCCTAAAGAC GACATTTGAG 
GACCTTATCC AGCGCTGCCT CTCCTCCGCT ACAGACCCCC AAACCAAGAG GAAGCTGGAC 
GACGCCAGCA AGCGCCTGGA GTTCCTCTAC GACAAGCTTA GGGAACAGAC ACTCTCCCCG 
ACCATCATCA ACGGTCTCCA CAGCATCGCC AGGAGCATTG AGACGAGGAA CTACTCGGAA 
GGGTTGAGCG TCCACACTCA CATCGTCAGC ACCAGCAACT TCAGTGAGAC CTCGGCCTTC 
ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247863.1
CDS	173..3583
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQRPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249561.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249561.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCGTCC TCAGAATGGT TGGAATGATC CTCCAGCTCT GAACAGAGTG 
CCGAAGAAGA AGAAGATGCC TGAAAACTTC ATGCCTCCTG TTCCCATTAC GTCACCAATC 
ATGAACCCTT CGGGTGATCC CCAGTCACAG GGGCTGCAGC AGCAGCCTTC AACTCCAGGG 
CCGCTGTCAA GCCACGCCTC CTTCCCACAG CAGCACCTAG CGGGCGGCCA GCCCTTCCAT 
GGTGTACAGC AGCCTCTTGC TCAGACAGGC ATGCCGCCGT CCTTCTCAAA GCCCAACACT 
GAAGGGGCTC CAGGAGCTCC CATTGGAAAC ACCATCCAGC ACGTGCAGGC TCTGCCAACA 
GAAAAGATTA CTAAGAAACC TATTCCGGAG GAGCACCTCA TCCTAAAGAC GACATTTGAG 
GACCTTATCC AGCGCTGCCT CTCCTCCGCT ACAGACCCCC AAACCAAGAG GAAGCTGGAC 
GACGCCAGCA AGCGCCTGGA GTTCCTCTAC GACAAGCTTA GGGAACAGAC ACTCTCCCCG 
ACCATCATCA ACGGTCTCCA CAGCATCGCC AGGAGCATTG AGACGAGGAA CTACTCGGAA 
GGGTTGAGCG TCCACACTCA CATCGTCAGC ACCAGCAACT TCAGTGAGAC CTCGGCCTTC 
ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249562.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247864.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu70394
Clone ID Related Accession (Same CDS sequence)	XM_011249562.2 , XM_011249562.3
Accession Version	XM_011249562.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCGTCCTCA GAATGGTTGG 
AATGATCCTC CAGCTCTGAA CAGAGTGCCG AAGAAGAAGA AGATGCCTGA AAACTTCATG 
CCTCCTGTTC CCATTACGTC ACCAATCATG AACCCTTCGG GTGATCCCCA GTCACAGGGG 
CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG CTGTCAAGCC ACGCCTCCTT CCCACAGCAG 
CACCTAGCGG GCGGCCAGCC CTTCCATGGT GTACAGCAGC CTCTTGCTCA GACAGGCATG 
CCGCCGTCCT TCTCAAAGCC CAACACTGAA GGGGCTCCAG GAGCTCCCAT TGGAAACACC 
ATCCAGCACG TGCAGGCTCT GCCAACAGAA AAGATTACTA AGAAACCTAT TCCGGAGGAG 
CACCTCATCC TAAAGACGAC ATTTGAGGAC CTTATCCAGC GCTGCCTCTC CTCCGCTACA 
GACCCCCAAA CCAAGAGGAA GCTGGACGAC GCCAGCAAGC GCCTGGAGTT CCTCTACGAC 
AAGCTTAGGG AACAGACACT CTCCCCGACC ATCATCAACG GTCTCCACAG CATCGCCAGG 
AGCATTGAGA CGAGGAACTA CTCGGAAGGG TTGAGCGTCC ACACTCACAT CGTCAGCACC 
AGCAACTTCA GTGAGACCTC GGCCTTCATG CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC 
AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247864.1
CDS	173..3490
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQRPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249562.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249562.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCGTCCTCA GAATGGTTGG 
AATGATCCTC CAGCTCTGAA CAGAGTGCCG AAGAAGAAGA AGATGCCTGA AAACTTCATG 
CCTCCTGTTC CCATTACGTC ACCAATCATG AACCCTTCGG GTGATCCCCA GTCACAGGGG 
CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG CTGTCAAGCC ACGCCTCCTT CCCACAGCAG 
CACCTAGCGG GCGGCCAGCC CTTCCATGGT GTACAGCAGC CTCTTGCTCA GACAGGCATG 
CCGCCGTCCT TCTCAAAGCC CAACACTGAA GGGGCTCCAG GAGCTCCCAT TGGAAACACC 
ATCCAGCACG TGCAGGCTCT GCCAACAGAA AAGATTACTA AGAAACCTAT TCCGGAGGAG 
CACCTCATCC TAAAGACGAC ATTTGAGGAC CTTATCCAGC GCTGCCTCTC CTCCGCTACA 
GACCCCCAAA CCAAGAGGAA GCTGGACGAC GCCAGCAAGC GCCTGGAGTT CCTCTACGAC 
AAGCTTAGGG AACAGACACT CTCCCCGACC ATCATCAACG GTCTCCACAG CATCGCCAGG 
AGCATTGAGA CGAGGAACTA CTCGGAAGGG TTGAGCGTCC ACACTCACAT CGTCAGCACC 
AGCAACTTCA GTGAGACCTC GGCCTTCATG CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC 
AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249563.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247865.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X13, mRNA.

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CloneID	OMu70395
Clone ID Related Accession (Same CDS sequence)	XM_011249563.2 , XM_011249563.3
Accession Version	XM_011249563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247865.1
CDS	150..3350
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQRPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249563.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249563.3

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( NM_026969.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081245.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 2, mRNA.

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CloneID	OMu17676
Clone ID Related Accession (Same CDS sequence)	NM_026969.1
Accession Version	NM_026969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143455.1, AC107744.8 and BU755387.1. On or before Jan 3, 2018 this sequence version replaced XM_011249554.2, XM_907601.3, XM_132230.8. Transcript Variant: This variant (2) differs in the 5' UTR, lacks an alternate in-frame exon, contains an alternate in-frame exon, and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGA AAATCAATCT TTCCAAGACC AGGCATCTAT ATTGGAAGGT 
CCTCAGAATG GTTGGAATGA TCCTCCAGCT CTGAACAGAG TGCCGAAGAA GAAGAAGATG 
CCTGAAAACT TCATGCCTCC TGTTCCCATT ACGTCACCAA TCATGAACCC TTCGGGTGAT 
CCCCAGTCAC AGGGGCTGCA GCAGCAGCCT TCAACTCCAG GGCCGCTGTC AAGCCACGCC 
TCCTTCCCAC AGCAGCACCT AGCGGGCGGC CAGCCCTTCC ATGGTGTACA GCAGCCTCTT 
GCTCAGACAG GCATGCCGCC GTCCTTCTCA AAGCCCAACA CTGAAGGGGC TCCAGGAGCT 
CCCATTGGAA ACACCATCCA GCACGTGCAG GCTCTGCCAA CAGAAAAGAT TACTAAGAAA 
CCTATTCCGG AGGAGCACCT CATCCTAAAG ACGACATTTG AGGACCTTAT CCAGCGCTGC 
CTCTCCTCCG CTACAGACCC CCAAACCAAG AGGAAGCTGG ACGACGCCAG CAAGCGCCTG 
GAGTTCCTCT ACGACAAGCT TAGGGAACAG ACACTCTCCC CGACCATCAT CAACGGTCTC 
CACAGCATCG CCAGGAGCAT TGAGACGAGG AACTACTCGG AAGGGTTGAG CGTCCACACT 
CACATCGTCA GCACCAGCAA CTTCAGTGAG ACCTCGGCCT TCATGCCCGT CCTCAAGGTT 
GTCCTCAGTC AGGCCAGCAA GCTGGGTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081245.1
CDS	134..3826
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	NM_026969.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026969.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGA AAATCAATCT TTCCAAGACC AGGCATCTAT ATTGGAAGGT 
CCTCAGAATG GTTGGAATGA TCCTCCAGCT CTGAACAGAG TGCCGAAGAA GAAGAAGATG 
CCTGAAAACT TCATGCCTCC TGTTCCCATT ACGTCACCAA TCATGAACCC TTCGGGTGAT 
CCCCAGTCAC AGGGGCTGCA GCAGCAGCCT TCAACTCCAG GGCCGCTGTC AAGCCACGCC 
TCCTTCCCAC AGCAGCACCT AGCGGGCGGC CAGCCCTTCC ATGGTGTACA GCAGCCTCTT 
GCTCAGACAG GCATGCCGCC GTCCTTCTCA AAGCCCAACA CTGAAGGGGC TCCAGGAGCT 
CCCATTGGAA ACACCATCCA GCACGTGCAG GCTCTGCCAA CAGAAAAGAT TACTAAGAAA 
CCTATTCCGG AGGAGCACCT CATCCTAAAG ACGACATTTG AGGACCTTAT CCAGCGCTGC 
CTCTCCTCCG CTACAGACCC CCAAACCAAG AGGAAGCTGG ACGACGCCAG CAAGCGCCTG 
GAGTTCCTCT ACGACAAGCT TAGGGAACAG ACACTCTCCC CGACCATCAT CAACGGTCTC 
CACAGCATCG CCAGGAGCAT TGAGACGAGG AACTACTCGG AAGGGTTGAG CGTCCACACT 
CACATCGTCA GCACCAGCAA CTTCAGTGAG ACCTCGGCCT TCATGCCCGT CCTCAAGGTT 
GTCCTCAGTC AGGCCAGCAA GCTGGGTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( NM_001359323.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346252.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 1, mRNA.

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CloneID	OMu70386
Clone ID Related Accession (Same CDS sequence)	XM_011249554.2 , NM_001359323.1
Accession Version	NM_001359323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107744.8. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.83697.1, SRR1660817.316075.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTC CTCAGAATGG TTGGAATGAT CCTCCAGCTC TGAACAGAGT GCCGAAGAAG 
AAGAAGATGC CTGAAAACTT CATGCCTCCT GTTCCCATTA CGTCACCAAT CATGAACCCT 
TCGGGTGATC CCCAGTCACA GGGGCTGCAG CAGCAGCCTT CAACTCCAGG GCCGCTGTCA 
AGCCACGCCT CCTTCCCACA GCAGCACCTA GCGGGCGGCC AGCCCTTCCA TGGTGTACAG 
CAGCCTCTTG CTCAGACAGG CATGCCGCCG TCCTTCTCAA AGCCCAACAC TGAAGGGGCT 
CCAGGAGCTC CCATTGGAAA CACCATCCAG CACGTGCAGG CTCTGCCAAC AGAAAAGATT 
ACTAAGAAAC CTATTCCGGA GGAGCACCTC ATCCTAAAGA CGACATTTGA GGACCTTATC 
CAGCGCTGCC TCTCCTCCGC TACAGACCCC CAAACCAAGA GGAAGCTGGA CGACGCCAGC 
AAGCGCCTGG AGTTCCTCTA CGACAAGCTT AGGGAACAGA CACTCTCCCC GACCATCATC 
AACGGTCTCC ACAGCATCGC CAGGAGCATT GAGACGAGGA ACTACTCGGA AGGGTTGAGC 
GTCCACACTC ACATCGTCAG CACCAGCAAC TTCAGTGAGA CCTCGGCCTT CATGCCCGTC 
CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346252.1
CDS	134..3835
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	NM_001359323.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359323.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTC CTCAGAATGG TTGGAATGAT CCTCCAGCTC TGAACAGAGT GCCGAAGAAG 
AAGAAGATGC CTGAAAACTT CATGCCTCCT GTTCCCATTA CGTCACCAAT CATGAACCCT 
TCGGGTGATC CCCAGTCACA GGGGCTGCAG CAGCAGCCTT CAACTCCAGG GCCGCTGTCA 
AGCCACGCCT CCTTCCCACA GCAGCACCTA GCGGGCGGCC AGCCCTTCCA TGGTGTACAG 
CAGCCTCTTG CTCAGACAGG CATGCCGCCG TCCTTCTCAA AGCCCAACAC TGAAGGGGCT 
CCAGGAGCTC CCATTGGAAA CACCATCCAG CACGTGCAGG CTCTGCCAAC AGAAAAGATT 
ACTAAGAAAC CTATTCCGGA GGAGCACCTC ATCCTAAAGA CGACATTTGA GGACCTTATC 
CAGCGCTGCC TCTCCTCCGC TACAGACCCC CAAACCAAGA GGAAGCTGGA CGACGCCAGC 
AAGCGCCTGG AGTTCCTCTA CGACAAGCTT AGGGAACAGA CACTCTCCCC GACCATCATC 
AACGGTCTCC ACAGCATCGC CAGGAGCATT GAGACGAGGA ACTACTCGGA AGGGTTGAGC 
GTCCACACTC ACATCGTCAG CACCAGCAAC TTCAGTGAGA CCTCGGCCTT CATGCCCGTC 
CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( NM_001359348.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346277.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 5, mRNA.

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CloneID	OMu70388
Clone ID Related Accession (Same CDS sequence)	XM_017321088.1 , NM_001359348.1 , NM_001359344.1 , XM_011249556.2
Accession Version	NM_001359348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107744.8. On Jan 3, 2018 this sequence version replaced XM_017321088.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks an alternate in-frame exon, and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (d) has the same N- and C-termini but is shorter compared to isoform a. Variants 4 and 5 both encode the same isoform (d). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.35885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346277.1
CDS	148..3801
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	NM_001359348.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359348.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( NM_001359344.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346273.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu70388
Clone ID Related Accession (Same CDS sequence)	XM_017321088.1 , NM_001359348.1 , NM_001359344.1 , XM_011249556.2
Accession Version	NM_001359344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107744.8. On Jan 3, 2018 this sequence version replaced XM_011249556.2. Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (d) has the same N- and C-termini but is shorter compared to isoform a. Variants 4 and 5 both encode the same isoform (d). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.145711.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346273.1
CDS	134..3787
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	NM_001359344.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359344.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( NM_001359350.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346279.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 7, mRNA.

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CloneID	OMu70389
Clone ID Related Accession (Same CDS sequence)	NM_001359350.1 , XM_011249557.2 , XM_017321089.1 , NM_001359332.1
Accession Version	NM_001359350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107744.8. On Jan 3, 2018 this sequence version replaced XM_017321089.1. Transcript Variant: This variant (7) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Variants 3 and 7 both encode the same isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.245469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346279.1
CDS	94..3702
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	NM_001359350.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359350.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( NM_001359332.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346261.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 3, mRNA.

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CloneID	OMu70389
Clone ID Related Accession (Same CDS sequence)	NM_001359350.1 , XM_011249557.2 , XM_017321089.1 , NM_001359332.1
Accession Version	NM_001359332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107744.8. On Jan 3, 2018 this sequence version replaced XM_011249557.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Variants 3 and 7 both encode the same isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.4631453.1, SRR7974084.7559.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346261.1
CDS	134..3742
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	NM_001359332.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359332.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_006535192.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535255.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X1, mRNA.

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CloneID	OMu35648
Clone ID Related Accession (Same CDS sequence)	XM_006535192.2 , XM_006535193.2 , XM_006535193.1 , XM_006535192.3 , XM_006535194.2
Accession Version	XM_006535192.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535255.1
CDS	151..3936
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_006535192.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535192.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_006535193.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535256.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X2, mRNA.

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CloneID	OMu35648
Clone ID Related Accession (Same CDS sequence)	XM_006535192.2 , XM_006535193.2 , XM_006535193.1 , XM_006535192.3 , XM_006535194.2
Accession Version	XM_006535193.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535256.1
CDS	185..3970
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_006535193.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535193.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_006535194.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535257.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X3, mRNA.

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CloneID	OMu35648
Clone ID Related Accession (Same CDS sequence)	XM_006535192.2 , XM_006535193.2 , XM_006535193.1 , XM_006535192.3 , XM_006535194.2
Accession Version	XM_006535194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535257.1
CDS	152..3937
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_006535194.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535194.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGAAAATCAA 
TCTTTCCAAG ACCAGGCATC TATATTGGAA GGTCCTCAGA ATGGTTGGAA TGATCCTCCA 
GCTCTGAACA GAGTGCCGAA GAAGAAGAAG ATGCCTGAAA ACTTCATGCC TCCTGTTCCC 
ATTACGTCAC CAATCATGAA CCCTTCGGGT GATCCCCAGT CACAGGGGCT GCAGCAGCAG 
CCTTCAACTC CAGGGCCGCT GTCAAGCCAC GCCTCCTTCC CACAGCAGCA CCTAGCGGGC 
GGCCAGCCCT TCCATGGTGT ACAGCAGCCT CTTGCTCAGA CAGGCATGCC GCCGTCCTTC 
TCAAAGCCCA ACACTGAAGG GGCTCCAGGA GCTCCCATTG GAAACACCAT CCAGCACGTG 
CAGGCTCTGC CAACAGAAAA GATTACTAAG AAACCTATTC CGGAGGAGCA CCTCATCCTA 
AAGACGACAT TTGAGGACCT TATCCAGCGC TGCCTCTCCT CCGCTACAGA CCCCCAAACC 
AAGAGGAAGC TGGACGACGC CAGCAAGCGC CTGGAGTTCC TCTACGACAA GCTTAGGGAA 
CAGACACTCT CCCCGACCAT CATCAACGGT CTCCACAGCA TCGCCAGGAG CATTGAGACG 
AGGAACTACT CGGAAGGGTT GAGCGTCCAC ACTCACATCG TCAGCACCAG CAACTTCAGT 
GAGACCTCGG CCTTCATGCC CGTCCTCAAG GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249551.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247853.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X4, mRNA.

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CloneID	OMu70383
Clone ID Related Accession (Same CDS sequence)	XM_011249551.2 , XM_011249551.3
Accession Version	XM_011249551.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG 
AATGGTTGGA ATGATCCTCC AGCTCTGAAC AGAGTGCCGA AGAAGAAGAA GATGCCTGAA 
AACTTCATGC CTCCTGTTCC CATTACGTCA CCAATCATGA ACCCTTCGGG TGATCCCCAG 
TCACAGGGGC TGCAGCAGCA GCCTTCAACT CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC 
CCACAGCAGC ACCTAGCGGG CGGCCAGCCC TTCCATGGTG TACAGCAGCC TCTTGCTCAG 
ACAGGCATGC CGCCGTCCTT CTCAAAGCCC AACACTGAAG GGGCTCCAGG AGCTCCCATT 
GGAAACACCA TCCAGCACGT GCAGGCTCTG CCAACAGAAA AGATTACTAA GAAACCTATT 
CCGGAGGAGC ACCTCATCCT AAAGACGACA TTTGAGGACC TTATCCAGCG CTGCCTCTCC 
TCCGCTACAG ACCCCCAAAC CAAGAGGAAG CTGGACGACG CCAGCAAGCG CCTGGAGTTC 
CTCTACGACA AGCTTAGGGA ACAGACACTC TCCCCGACCA TCATCAACGG TCTCCACAGC 
ATCGCCAGGA GCATTGAGAC GAGGAACTAC TCGGAAGGGT TGAGCGTCCA CACTCACATC 
GTCAGCACCA GCAACTTCAG TGAGACCTCG GCCTTCATGC CCGTCCTCAA GGTTGTCCTC 
AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247853.1
CDS	151..3897
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249551.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249551.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTA CACCGCCTGC TGCCAGTGAG CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG 
AATGGTTGGA ATGATCCTCC AGCTCTGAAC AGAGTGCCGA AGAAGAAGAA GATGCCTGAA 
AACTTCATGC CTCCTGTTCC CATTACGTCA CCAATCATGA ACCCTTCGGG TGATCCCCAG 
TCACAGGGGC TGCAGCAGCA GCCTTCAACT CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC 
CCACAGCAGC ACCTAGCGGG CGGCCAGCCC TTCCATGGTG TACAGCAGCC TCTTGCTCAG 
ACAGGCATGC CGCCGTCCTT CTCAAAGCCC AACACTGAAG GGGCTCCAGG AGCTCCCATT 
GGAAACACCA TCCAGCACGT GCAGGCTCTG CCAACAGAAA AGATTACTAA GAAACCTATT 
CCGGAGGAGC ACCTCATCCT AAAGACGACA TTTGAGGACC TTATCCAGCG CTGCCTCTCC 
TCCGCTACAG ACCCCCAAAC CAAGAGGAAG CTGGACGACG CCAGCAAGCG CCTGGAGTTC 
CTCTACGACA AGCTTAGGGA ACAGACACTC TCCCCGACCA TCATCAACGG TCTCCACAGC 
ATCGCCAGGA GCATTGAGAC GAGGAACTAC TCGGAAGGGT TGAGCGTCCA CACTCACATC 
GTCAGCACCA GCAACTTCAG TGAGACCTCG GCCTTCATGC CCGTCCTCAA GGTTGTCCTC 
AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249552.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247854.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu70384
Clone ID Related Accession (Same CDS sequence)	XM_011249552.2 , XM_011249552.3
Accession Version	XM_011249552.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGAAA ATCAATCTTT CCAAGACCAG GCATCTATAT TGGAAGGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247854.1
CDS	151..3891
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249552.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249552.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGAAA ATCAATCTTT CCAAGACCAG GCATCTATAT TGGAAGGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249553.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247855.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X6, mRNA.

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CloneID	OMu70385
Clone ID Related Accession (Same CDS sequence)	XM_011249553.2 , XM_011249553.3
Accession Version	XM_011249553.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGCGCAT TAAAGAGGAA 
AAACAGGAAT GTGACTTTCT GCCCTCGGCG GGAGGGACGT TTAACATCTC TGTCAGTGGG 
GATATTGACG GTCTAATTAC CCGGGCCTTG CTGACAGGCA ATTTTGAGAG TGCAGTTGAC 
CTTTGCTTAC ATGATAACCG AATGGCTGAT GCCATTATAT TAGCCATAGC AGGTGGACAG 
GAACTCTTGG CTCAAACACA AAAGAAATAC TTCGCAAAAT CCCAAAGCAA AATTACCAGG 
CTTATCACTG CAGTGGTGAT GAAGAACTGG AGGGAGATTG TTGAATCCTG TGATCTTAAG 
AATTGGAGAG AGGCTTTAGC TGCAGTCCTC ACTTACGCTA AACCAGACGA GTTCTCGGCC 
CTGTGCGATC TCCTGGGAAC CCGTCTGGAA AGAGAAGGAG ACAGCCTCCT GCGGACTCAG 
GCCTGCCTCT GCTACATCTG TGCAGGGAAT GTAGAGAGAT TGGTTGCTTG CTGGACAAAA 
GCTCAGGACG GCAGCAGCCC CTTGTCTCTC CAGGATCTGA TTGAGAAAGT TGTCATCCTC 
CGCAAAGCTG TACAGCTCAC CCAAGCCTTG GACACCAATA CTGTCGGGGC TCTTCTGGCT 
GAGAAGATGA GTCAGTATGC CAGCTTGTTG GCAGCCCAGG GCAGCATTGC CGCAGCCTTG 
GCTTTTCTGC CTGACAACAC CAACCAGCCA AATATTGTGC AGCTGCGTGA CAGACTCTGT 
AAAGCTCAAG GCAAGCCTGT GTCGGGACAG GAATCTTCTC AGAGCCCATA TGAGAGGCAG 
CCACTGTCCA AGGGCAGGCC TGGACCAGTT GCTGGCCACT CGCAGATGCC CAGAGTTCAA 
ACTCAGCAAT ATTATCCCCA TGTTAGAATT GCCCCTACTG TCACTACCTG GAGTAACAAA 
ACTCCCACTG CCCTCCCCAG CCATCCACCT GCAGCCTCTC CCTCTGACAC ACAGGGAGAA 
AATCCCCCAC CTCCAGGTTT CATAATGCAG GGCAATGTCA TTCCAAACCC TGCTGCCCCG 
CTGCCCACGG CTCCAGGCCA CATGCCCAGC CAGCTGCCAC CTTACCCACA GCCGCAGCCT 
TACCAGCCAG CCCAGCAGTA TTCCTTCGGA ACAGGGGGGG CAGCAGCTTA TCGACCTCAA 
CAGCCTGTTG CTCCTCCTGC TTCTAACGCT TACCCTAACA CCCCGTACAT ATCTCCTGTG 
GCTTCCTATT CTGGGCAGCC TCAGATGTAC ACAGCCCAGC AAGCCTCCTC ACCTACCTCC 
AGCTCTGCTG CTTCTTTCCC CCCTCCCTCC TCTGGAGCAT CCTTCCAGCA TGGCGGACCA 
GGAGCTCCAC CATCATCTTC AGCTTATGCA CTGCCTCCTG GAACAACAGG TACACCGCCT 
GCTGCCAGTG AGCTGCCTGC GTCCCAAAGA ACAGAAAATC AATCTTTCCA AGACCAGGCA 
TCTATATTGG AAGGTCCTCA GAATGGTTGG AATGATCCTC CAGCTCTGAA CAGAGTGCCG 
AAGAAGAAGA AGATGCCTGA AAACTTCATG CCTCCTGTTC CCATTACGTC ACCAATCATG 
AACCCTTCGG GTGATCCCCA GTCACAGGGG CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG 
CTGTCAAGCC ACGCCTCCTT CCCACAGCAG CACCTAGCGG GCGGCCAGCC CTTCCATGGT 
GTACAGCAGC CTCTTGCTCA GACAGGCATG CCGCCGTCCT TCTCAAAGCC CAACACTGAA 
GGGGCTCCAG GAGCTCCCAT TGGAAACACC ATCCAGCACG TGCAGGCTCT GCCAACAGAA 
AAGATTACTA AGAAACCTAT TCCGGAGGAG CACCTCATCC TAAAGACGAC ATTTGAGGAC 
CTTATCCAGC GCTGCCTCTC CTCCGCTACA GACCCCCAAA CCAAGAGGAA GCTGGACGAC 
GCCAGCAAGC GCCTGGAGTT CCTCTACGAC AAGCTTAGGG AACAGACACT CTCCCCGACC 
ATCATCAACG GTCTCCACAG CATCGCCAGG AGCATTGAGA CGAGGAACTA CTCGGAAGGG 
TTGAGCGTCC ACACTCACAT CGTCAGCACC AGCAACTTCA GTGAGACCTC GGCCTTCATG 
CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247855.1
CDS	151..3858
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249553.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249553.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGCGCAT TAAAGAGGAA 
AAACAGGAAT GTGACTTTCT GCCCTCGGCG GGAGGGACGT TTAACATCTC TGTCAGTGGG 
GATATTGACG GTCTAATTAC CCGGGCCTTG CTGACAGGCA ATTTTGAGAG TGCAGTTGAC 
CTTTGCTTAC ATGATAACCG AATGGCTGAT GCCATTATAT TAGCCATAGC AGGTGGACAG 
GAACTCTTGG CTCAAACACA AAAGAAATAC TTCGCAAAAT CCCAAAGCAA AATTACCAGG 
CTTATCACTG CAGTGGTGAT GAAGAACTGG AGGGAGATTG TTGAATCCTG TGATCTTAAG 
AATTGGAGAG AGGCTTTAGC TGCAGTCCTC ACTTACGCTA AACCAGACGA GTTCTCGGCC 
CTGTGCGATC TCCTGGGAAC CCGTCTGGAA AGAGAAGGAG ACAGCCTCCT GCGGACTCAG 
GCCTGCCTCT GCTACATCTG TGCAGGGAAT GTAGAGAGAT TGGTTGCTTG CTGGACAAAA 
GCTCAGGACG GCAGCAGCCC CTTGTCTCTC CAGGATCTGA TTGAGAAAGT TGTCATCCTC 
CGCAAAGCTG TACAGCTCAC CCAAGCCTTG GACACCAATA CTGTCGGGGC TCTTCTGGCT 
GAGAAGATGA GTCAGTATGC CAGCTTGTTG GCAGCCCAGG GCAGCATTGC CGCAGCCTTG 
GCTTTTCTGC CTGACAACAC CAACCAGCCA AATATTGTGC AGCTGCGTGA CAGACTCTGT 
AAAGCTCAAG GCAAGCCTGT GTCGGGACAG GAATCTTCTC AGAGCCCATA TGAGAGGCAG 
CCACTGTCCA AGGGCAGGCC TGGACCAGTT GCTGGCCACT CGCAGATGCC CAGAGTTCAA 
ACTCAGCAAT ATTATCCCCA TGTTAGAATT GCCCCTACTG TCACTACCTG GAGTAACAAA 
ACTCCCACTG CCCTCCCCAG CCATCCACCT GCAGCCTCTC CCTCTGACAC ACAGGGAGAA 
AATCCCCCAC CTCCAGGTTT CATAATGCAG GGCAATGTCA TTCCAAACCC TGCTGCCCCG 
CTGCCCACGG CTCCAGGCCA CATGCCCAGC CAGCTGCCAC CTTACCCACA GCCGCAGCCT 
TACCAGCCAG CCCAGCAGTA TTCCTTCGGA ACAGGGGGGG CAGCAGCTTA TCGACCTCAA 
CAGCCTGTTG CTCCTCCTGC TTCTAACGCT TACCCTAACA CCCCGTACAT ATCTCCTGTG 
GCTTCCTATT CTGGGCAGCC TCAGATGTAC ACAGCCCAGC AAGCCTCCTC ACCTACCTCC 
AGCTCTGCTG CTTCTTTCCC CCCTCCCTCC TCTGGAGCAT CCTTCCAGCA TGGCGGACCA 
GGAGCTCCAC CATCATCTTC AGCTTATGCA CTGCCTCCTG GAACAACAGG TACACCGCCT 
GCTGCCAGTG AGCTGCCTGC GTCCCAAAGA ACAGAAAATC AATCTTTCCA AGACCAGGCA 
TCTATATTGG AAGGTCCTCA GAATGGTTGG AATGATCCTC CAGCTCTGAA CAGAGTGCCG 
AAGAAGAAGA AGATGCCTGA AAACTTCATG CCTCCTGTTC CCATTACGTC ACCAATCATG 
AACCCTTCGG GTGATCCCCA GTCACAGGGG CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG 
CTGTCAAGCC ACGCCTCCTT CCCACAGCAG CACCTAGCGG GCGGCCAGCC CTTCCATGGT 
GTACAGCAGC CTCTTGCTCA GACAGGCATG CCGCCGTCCT TCTCAAAGCC CAACACTGAA 
GGGGCTCCAG GAGCTCCCAT TGGAAACACC ATCCAGCACG TGCAGGCTCT GCCAACAGAA 
AAGATTACTA AGAAACCTAT TCCGGAGGAG CACCTCATCC TAAAGACGAC ATTTGAGGAC 
CTTATCCAGC GCTGCCTCTC CTCCGCTACA GACCCCCAAA CCAAGAGGAA GCTGGACGAC 
GCCAGCAAGC GCCTGGAGTT CCTCTACGAC AAGCTTAGGG AACAGACACT CTCCCCGACC 
ATCATCAACG GTCTCCACAG CATCGCCAGG AGCATTGAGA CGAGGAACTA CTCGGAAGGG 
TTGAGCGTCC ACACTCACAT CGTCAGCACC AGCAACTTCA GTGAGACCTC GGCCTTCATG 
CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249554.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247856.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X7, mRNA.

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CloneID	OMu70386
Clone ID Related Accession (Same CDS sequence)	XM_011249554.2 , NM_001359323.1
Accession Version	XM_011249554.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein transport protein Sec31A isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTC CTCAGAATGG TTGGAATGAT CCTCCAGCTC TGAACAGAGT GCCGAAGAAG 
AAGAAGATGC CTGAAAACTT CATGCCTCCT GTTCCCATTA CGTCACCAAT CATGAACCCT 
TCGGGTGATC CCCAGTCACA GGGGCTGCAG CAGCAGCCTT CAACTCCAGG GCCGCTGTCA 
AGCCACGCCT CCTTCCCACA GCAGCACCTA GCGGGCGGCC AGCCCTTCCA TGGTGTACAG 
CAGCCTCTTG CTCAGACAGG CATGCCGCCG TCCTTCTCAA AGCCCAACAC TGAAGGGGCT 
CCAGGAGCTC CCATTGGAAA CACCATCCAG CACGTGCAGG CTCTGCCAAC AGAAAAGATT 
ACTAAGAAAC CTATTCCGGA GGAGCACCTC ATCCTAAAGA CGACATTTGA GGACCTTATC 
CAGCGCTGCC TCTCCTCCGC TACAGACCCC CAAACCAAGA GGAAGCTGGA CGACGCCAGC 
AAGCGCCTGG AGTTCCTCTA CGACAAGCTT AGGGAACAGA CACTCTCCCC GACCATCATC 
AACGGTCTCC ACAGCATCGC CAGGAGCATT GAGACGAGGA ACTACTCGGA AGGGTTGAGC 
GTCCACACTC ACATCGTCAG CACCAGCAAC TTCAGTGAGA CCTCGGCCTT CATGCCCGTC 
CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247856.1
CDS	133..3834
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249554.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249554.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCCTTA CCAGCCAGCC CAGCAGTATT CCTTCGGAAC AGGGGGGGCA 
GCAGCTTATC GACCTCAACA GCCTGTTGCT CCTCCTGCTT CTAACGCTTA CCCTAACACC 
CCGTACATAT CTCCTGTGGC TTCCTATTCT GGGCAGCCTC AGATGTACAC AGCCCAGCAA 
GCCTCCTCAC CTACCTCCAG CTCTGCTGCT TCTTTCCCCC CTCCCTCCTC TGGAGCATCC 
TTCCAGCATG GCGGACCAGG AGCTCCACCA TCATCTTCAG CTTATGCACT GCCTCCTGGA 
ACAACAGGTC CTCAGAATGG TTGGAATGAT CCTCCAGCTC TGAACAGAGT GCCGAAGAAG 
AAGAAGATGC CTGAAAACTT CATGCCTCCT GTTCCCATTA CGTCACCAAT CATGAACCCT 
TCGGGTGATC CCCAGTCACA GGGGCTGCAG CAGCAGCCTT CAACTCCAGG GCCGCTGTCA 
AGCCACGCCT CCTTCCCACA GCAGCACCTA GCGGGCGGCC AGCCCTTCCA TGGTGTACAG 
CAGCCTCTTG CTCAGACAGG CATGCCGCCG TCCTTCTCAA AGCCCAACAC TGAAGGGGCT 
CCAGGAGCTC CCATTGGAAA CACCATCCAG CACGTGCAGG CTCTGCCAAC AGAAAAGATT 
ACTAAGAAAC CTATTCCGGA GGAGCACCTC ATCCTAAAGA CGACATTTGA GGACCTTATC 
CAGCGCTGCC TCTCCTCCGC TACAGACCCC CAAACCAAGA GGAAGCTGGA CGACGCCAGC 
AAGCGCCTGG AGTTCCTCTA CGACAAGCTT AGGGAACAGA CACTCTCCCC GACCATCATC 
AACGGTCTCC ACAGCATCGC CAGGAGCATT GAGACGAGGA ACTACTCGGA AGGGTTGAGC 
GTCCACACTC ACATCGTCAG CACCAGCAAC TTCAGTGAGA CCTCGGCCTT CATGCCCGTC 
CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249555.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247857.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X8, mRNA.

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CloneID	OMu70387
Clone ID Related Accession (Same CDS sequence)	XM_011249555.3 , XM_011249555.2
Accession Version	XM_011249555.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249555.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGTTAGAAT TGCCCCTACT 
GTCACTACCT GGAGTAACAA AACTCCCACT GCCCTCCCCA GCCATCCACC TGCAGCCTCT 
CCCTCTGACA CACAGGGAGA AAATCCCCCA CCTCCAGGTT TCATAATGCA GGGCAATGTC 
ATTCCAAACC CTGCTGCCCC GCTGCCCACG GCTCCAGGCC ACATGCCCAG CCAGCTGCCA 
CCTTACCCAC AGCCGCAGCC TTACCAGCCA GCCCAGCAGT ATTCCTTCGG AACAGGGGGG 
GCAGCAGCTT ATCGACCTCA ACAGCCTGTT GCTCCTCCTG CTTCTAACGC TTACCCTAAC 
ACCCCGTACA TATCTCCTGT GGCTTCCTAT TCTGGGCAGC CTCAGATGTA CACAGCCCAG 
CAAGCCTCCT CACCTACCTC CAGCTCTGCT GCTTCTTTCC CCCCTCCCTC CTCTGGAGCA 
TCCTTCCAGC ATGGCGGACC AGGAGCTCCA CCATCATCTT CAGCTTATGC ACTGCCTCCT 
GGAACAACAG GTACACCGCC TGCTGCCAGT GAGCTGCCTG CGTCCCAAAG AACAGAAAAT 
CAATCTTTCC AAGACCAGGC ATCTATATTG GAAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247857.1
CDS	151..3819
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249555.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249555.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGTTAGAAT TGCCCCTACT 
GTCACTACCT GGAGTAACAA AACTCCCACT GCCCTCCCCA GCCATCCACC TGCAGCCTCT 
CCCTCTGACA CACAGGGAGA AAATCCCCCA CCTCCAGGTT TCATAATGCA GGGCAATGTC 
ATTCCAAACC CTGCTGCCCC GCTGCCCACG GCTCCAGGCC ACATGCCCAG CCAGCTGCCA 
CCTTACCCAC AGCCGCAGCC TTACCAGCCA GCCCAGCAGT ATTCCTTCGG AACAGGGGGG 
GCAGCAGCTT ATCGACCTCA ACAGCCTGTT GCTCCTCCTG CTTCTAACGC TTACCCTAAC 
ACCCCGTACA TATCTCCTGT GGCTTCCTAT TCTGGGCAGC CTCAGATGTA CACAGCCCAG 
CAAGCCTCCT CACCTACCTC CAGCTCTGCT GCTTCTTTCC CCCCTCCCTC CTCTGGAGCA 
TCCTTCCAGC ATGGCGGACC AGGAGCTCCA CCATCATCTT CAGCTTATGC ACTGCCTCCT 
GGAACAACAG GTACACCGCC TGCTGCCAGT GAGCTGCCTG CGTCCCAAAG AACAGAAAAT 
CAATCTTTCC AAGACCAGGC ATCTATATTG GAAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_017321088.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176577.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X10, mRNA.

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CloneID	OMu70388
Clone ID Related Accession (Same CDS sequence)	XM_017321088.1 , NM_001359348.1 , NM_001359344.1 , XM_011249556.2
Accession Version	XM_017321088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein transport protein Sec31A isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176577.1
CDS	152..3805
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_017321088.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321088.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249556.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247858.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu70388
Clone ID Related Accession (Same CDS sequence)	XM_017321088.1 , NM_001359348.1 , NM_001359344.1 , XM_011249556.2
Accession Version	XM_011249556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein transport protein Sec31A isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247858.1
CDS	150..3803
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249556.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249556.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTACAC CGCCTGCTGC CAGTGAGCTG 
CCTGCGTCCC AAAGAACAGG TCCTCAGAAT GGTTGGAATG ATCCTCCAGC TCTGAACAGA 
GTGCCGAAGA AGAAGAAGAT GCCTGAAAAC TTCATGCCTC CTGTTCCCAT TACGTCACCA 
ATCATGAACC CTTCGGGTGA TCCCCAGTCA CAGGGGCTGC AGCAGCAGCC TTCAACTCCA 
GGGCCGCTGT CAAGCCACGC CTCCTTCCCA CAGCAGCACC TAGCGGGCGG CCAGCCCTTC 
CATGGTGTAC AGCAGCCTCT TGCTCAGACA GGCATGCCGC CGTCCTTCTC AAAGCCCAAC 
ACTGAAGGGG CTCCAGGAGC TCCCATTGGA AACACCATCC AGCACGTGCA GGCTCTGCCA 
ACAGAAAAGA TTACTAAGAA ACCTATTCCG GAGGAGCACC TCATCCTAAA GACGACATTT 
GAGGACCTTA TCCAGCGCTG CCTCTCCTCC GCTACAGACC CCCAAACCAA GAGGAAGCTG 
GACGACGCCA GCAAGCGCCT GGAGTTCCTC TACGACAAGC TTAGGGAACA GACACTCTCC 
CCGACCATCA TCAACGGTCT CCACAGCATC GCCAGGAGCA TTGAGACGAG GAACTACTCG 
GAAGGGTTGA GCGTCCACAC TCACATCGTC AGCACCAGCA ACTTCAGTGA GACCTCGGCC 
TTCATGCCCG TCCTCAAGGT TGTCCTCAGT CAGGCCAGCA AGCTGGGTGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249557.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247859.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X11, mRNA.

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CloneID	OMu70389
Clone ID Related Accession (Same CDS sequence)	NM_001359350.1 , XM_011249557.2 , XM_017321089.1 , NM_001359332.1
Accession Version	XM_011249557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein transport protein Sec31A isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247859.1
CDS	150..3758
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249557.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249557.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_017321089.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176578.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X12, mRNA.

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CloneID	OMu70389
Clone ID Related Accession (Same CDS sequence)	NM_001359350.1 , XM_011249557.2 , XM_017321089.1 , NM_001359332.1
Accession Version	XM_017321089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein transport protein Sec31A isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176578.1
CDS	152..3760
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_017321089.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321089.1

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCCTTACCA GCCAGCCCAG 
CAGTATTCCT TCGGAACAGG GGGGGCAGCA GCTTATCGAC CTCAACAGCC TGTTGCTCCT 
CCTGCTTCTA ACGCTTACCC TAACACCCCG TACATATCTC CTGTGGCTTC CTATTCTGGG 
CAGCCTCAGA TGTACACAGC CCAGCAAGCC TCCTCACCTA CCTCCAGCTC TGCTGCTTCT 
TTCCCCCCTC CCTCCTCTGG AGCATCCTTC CAGCATGGCG GACCAGGAGC TCCACCATCA 
TCTTCAGCTT ATGCACTGCC TCCTGGAACA ACAGGTCCTC AGAATGGTTG GAATGATCCT 
CCAGCTCTGA ACAGAGTGCC GAAGAAGAAG AAGATGCCTG AAAACTTCAT GCCTCCTGTT 
CCCATTACGT CACCAATCAT GAACCCTTCG GGTGATCCCC AGTCACAGGG GCTGCAGCAG 
CAGCCTTCAA CTCCAGGGCC GCTGTCAAGC CACGCCTCCT TCCCACAGCA GCACCTAGCG 
GGCGGCCAGC CCTTCCATGG TGTACAGCAG CCTCTTGCTC AGACAGGCAT GCCGCCGTCC 
TTCTCAAAGC CCAACACTGA AGGGGCTCCA GGAGCTCCCA TTGGAAACAC CATCCAGCAC 
GTGCAGGCTC TGCCAACAGA AAAGATTACT AAGAAACCTA TTCCGGAGGA GCACCTCATC 
CTAAAGACGA CATTTGAGGA CCTTATCCAG CGCTGCCTCT CCTCCGCTAC AGACCCCCAA 
ACCAAGAGGA AGCTGGACGA CGCCAGCAAG CGCCTGGAGT TCCTCTACGA CAAGCTTAGG 
GAACAGACAC TCTCCCCGAC CATCATCAAC GGTCTCCACA GCATCGCCAG GAGCATTGAG 
ACGAGGAACT ACTCGGAAGG GTTGAGCGTC CACACTCACA TCGTCAGCAC CAGCAACTTC 
AGTGAGACCT CGGCCTTCAT GCCCGTCCTC AAGGTTGTCC TCAGTCAGGC CAGCAAGCTG 
GGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249558.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247860.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X8, mRNA.

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CloneID	OMu70390
Clone ID Related Accession (Same CDS sequence)	XM_011249558.2
Accession Version	XM_011249558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTA CACCGCCTGC TGCCAGTGAG 
CTGCCTGCGT CCCAAAGAAC AGAAAATCAA TCTTTCCAAG ACCAGGCATC TATATTGGAA 
GGTCCTCAGA ATGGTTGGAA TGATCCTCCA GCTCTGAACA GAGTGCCGAA GAAGAAGAAG 
ATGCCTGAAA ACTTCATGCC TCCTGTTCCC ATTACGTCAC CAATCATGAA CCCTTCGGGT 
GATCCCCAGT CACAGGGGCT GCAGCAGCAG CCTTCAACTC CAGGGCCGCT GTCAAGCCAC 
GCCTCCTTCC CACAGCAGCA CCTAGCGGGC GGCCAGCCCT TCCATGGTGT ACAGCAGCCT 
CTTGCTCAGA CAGGCATGCC GCCGTCCTTC TCAAAGCCCA ACACTGAAGG GGCTCCAGGA 
GCTCCCATTG GAAACACCAT CCAGCACGTG CAGGCTCTGC CAACAGAAAA GATTACTAAG 
AAACCTATTC CGGAGGAGCA CCTCATCCTA AAGACGACAT TTGAGGACCT TATCCAGCGC 
TGCCTCTCCT CCGCTACAGA CCCCCAAACC AAGAGGAAGC TGGACGACGC CAGCAAGCGC 
CTGGAGTTCC TCTACGACAA GCTTAGGGAA CAGACACTCT CCCCGACCAT CATCAACGGT 
CTCCACAGCA TCGCCAGGAG CATTGAGACG AGGAACTACT CGGAAGGGTT GAGCGTCCAC 
ACTCACATCG TCAGCACCAG CAACTTCAGT GAGACCTCGG CCTTCATGCC CGTCCTCAAG 
GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247860.1
CDS	150..3725
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTENQSFQDQASILEGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249558.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249558.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTA CACCGCCTGC TGCCAGTGAG 
CTGCCTGCGT CCCAAAGAAC AGAAAATCAA TCTTTCCAAG ACCAGGCATC TATATTGGAA 
GGTCCTCAGA ATGGTTGGAA TGATCCTCCA GCTCTGAACA GAGTGCCGAA GAAGAAGAAG 
ATGCCTGAAA ACTTCATGCC TCCTGTTCCC ATTACGTCAC CAATCATGAA CCCTTCGGGT 
GATCCCCAGT CACAGGGGCT GCAGCAGCAG CCTTCAACTC CAGGGCCGCT GTCAAGCCAC 
GCCTCCTTCC CACAGCAGCA CCTAGCGGGC GGCCAGCCCT TCCATGGTGT ACAGCAGCCT 
CTTGCTCAGA CAGGCATGCC GCCGTCCTTC TCAAAGCCCA ACACTGAAGG GGCTCCAGGA 
GCTCCCATTG GAAACACCAT CCAGCACGTG CAGGCTCTGC CAACAGAAAA GATTACTAAG 
AAACCTATTC CGGAGGAGCA CCTCATCCTA AAGACGACAT TTGAGGACCT TATCCAGCGC 
TGCCTCTCCT CCGCTACAGA CCCCCAAACC AAGAGGAAGC TGGACGACGC CAGCAAGCGC 
CTGGAGTTCC TCTACGACAA GCTTAGGGAA CAGACACTCT CCCCGACCAT CATCAACGGT 
CTCCACAGCA TCGCCAGGAG CATTGAGACG AGGAACTACT CGGAAGGGTT GAGCGTCCAC 
ACTCACATCG TCAGCACCAG CAACTTCAGT GAGACCTCGG CCTTCATGCC CGTCCTCAAG 
GTTGTCCTCA GTCAGGCCAG CAAGCTGGGT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249559.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247861.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X14, mRNA.

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CloneID	OMu70391
Clone ID Related Accession (Same CDS sequence)	XM_011249559.2 , XM_011249559.3
Accession Version	XM_011249559.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTA CACCGCCTGC TGCCAGTGAG 
CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG AATGGTTGGA ATGATCCTCC AGCTCTGAAC 
AGAGTGCCGA AGAAGAAGAA GATGCCTGAA AACTTCATGC CTCCTGTTCC CATTACGTCA 
CCAATCATGA ACCCTTCGGG TGATCCCCAG TCACAGGGGC TGCAGCAGCA GCCTTCAACT 
CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC CCACAGCAGC ACCTAGCGGG CGGCCAGCCC 
TTCCATGGTG TACAGCAGCC TCTTGCTCAG ACAGGCATGC CGCCGTCCTT CTCAAAGCCC 
AACACTGAAG GGGCTCCAGG AGCTCCCATT GGAAACACCA TCCAGCACGT GCAGGCTCTG 
CCAACAGAAA AGATTACTAA GAAACCTATT CCGGAGGAGC ACCTCATCCT AAAGACGACA 
TTTGAGGACC TTATCCAGCG CTGCCTCTCC TCCGCTACAG ACCCCCAAAC CAAGAGGAAG 
CTGGACGACG CCAGCAAGCG CCTGGAGTTC CTCTACGACA AGCTTAGGGA ACAGACACTC 
TCCCCGACCA TCATCAACGG TCTCCACAGC ATCGCCAGGA GCATTGAGAC GAGGAACTAC 
TCGGAAGGGT TGAGCGTCCA CACTCACATC GTCAGCACCA GCAACTTCAG TGAGACCTCG 
GCCTTCATGC CCGTCCTCAA GGTTGTCCTC AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247861.1
CDS	150..3686
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGTPPAASELPASQRTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249559.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249559.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTA CACCGCCTGC TGCCAGTGAG 
CTGCCTGCGT CCCAAAGAAC AGGTCCTCAG AATGGTTGGA ATGATCCTCC AGCTCTGAAC 
AGAGTGCCGA AGAAGAAGAA GATGCCTGAA AACTTCATGC CTCCTGTTCC CATTACGTCA 
CCAATCATGA ACCCTTCGGG TGATCCCCAG TCACAGGGGC TGCAGCAGCA GCCTTCAACT 
CCAGGGCCGC TGTCAAGCCA CGCCTCCTTC CCACAGCAGC ACCTAGCGGG CGGCCAGCCC 
TTCCATGGTG TACAGCAGCC TCTTGCTCAG ACAGGCATGC CGCCGTCCTT CTCAAAGCCC 
AACACTGAAG GGGCTCCAGG AGCTCCCATT GGAAACACCA TCCAGCACGT GCAGGCTCTG 
CCAACAGAAA AGATTACTAA GAAACCTATT CCGGAGGAGC ACCTCATCCT AAAGACGACA 
TTTGAGGACC TTATCCAGCG CTGCCTCTCC TCCGCTACAG ACCCCCAAAC CAAGAGGAAG 
CTGGACGACG CCAGCAAGCG CCTGGAGTTC CTCTACGACA AGCTTAGGGA ACAGACACTC 
TCCCCGACCA TCATCAACGG TCTCCACAGC ATCGCCAGGA GCATTGAGAC GAGGAACTAC 
TCGGAAGGGT TGAGCGTCCA CACTCACATC GTCAGCACCA GCAACTTCAG TGAGACCTCG 
GCCTTCATGC CCGTCCTCAA GGTTGTCCTC AGTCAGGCCA GCAAGCTGGG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249560.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247862.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X15, mRNA.

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CloneID	OMu70392
Clone ID Related Accession (Same CDS sequence)	XM_011249560.2 , XM_011249560.3
Accession Version	XM_011249560.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTC CTCAGAATGG TTGGAATGAT 
CCTCCAGCTC TGAACAGAGT GCCGAAGAAG AAGAAGATGC CTGAAAACTT CATGCCTCCT 
GTTCCCATTA CGTCACCAAT CATGAACCCT TCGGGTGATC CCCAGTCACA GGGGCTGCAG 
CAGCAGCCTT CAACTCCAGG GCCGCTGTCA AGCCACGCCT CCTTCCCACA GCAGCACCTA 
GCGGGCGGCC AGCCCTTCCA TGGTGTACAG CAGCCTCTTG CTCAGACAGG CATGCCGCCG 
TCCTTCTCAA AGCCCAACAC TGAAGGGGCT CCAGGAGCTC CCATTGGAAA CACCATCCAG 
CACGTGCAGG CTCTGCCAAC AGAAAAGATT ACTAAGAAAC CTATTCCGGA GGAGCACCTC 
ATCCTAAAGA CGACATTTGA GGACCTTATC CAGCGCTGCC TCTCCTCCGC TACAGACCCC 
CAAACCAAGA GGAAGCTGGA CGACGCCAGC AAGCGCCTGG AGTTCCTCTA CGACAAGCTT 
AGGGAACAGA CACTCTCCCC GACCATCATC AACGGTCTCC ACAGCATCGC CAGGAGCATT 
GAGACGAGGA ACTACTCGGA AGGGTTGAGC GTCCACACTC ACATCGTCAG CACCAGCAAC 
TTCAGTGAGA CCTCGGCCTT CATGCCCGTC CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG 
CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247862.1
CDS	150..3641
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQPYQPAQQYSFGTGGAAAYRPQQPVAPPASNAYPNTPYISPVASYSGQPQMYTAQQASSPTSSSAASFPPPSSGASFQHGGPGAPPSSSAYALPPGTTGPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249560.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249560.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCCTTA CCAGCCAGCC 
CAGCAGTATT CCTTCGGAAC AGGGGGGGCA GCAGCTTATC GACCTCAACA GCCTGTTGCT 
CCTCCTGCTT CTAACGCTTA CCCTAACACC CCGTACATAT CTCCTGTGGC TTCCTATTCT 
GGGCAGCCTC AGATGTACAC AGCCCAGCAA GCCTCCTCAC CTACCTCCAG CTCTGCTGCT 
TCTTTCCCCC CTCCCTCCTC TGGAGCATCC TTCCAGCATG GCGGACCAGG AGCTCCACCA 
TCATCTTCAG CTTATGCACT GCCTCCTGGA ACAACAGGTC CTCAGAATGG TTGGAATGAT 
CCTCCAGCTC TGAACAGAGT GCCGAAGAAG AAGAAGATGC CTGAAAACTT CATGCCTCCT 
GTTCCCATTA CGTCACCAAT CATGAACCCT TCGGGTGATC CCCAGTCACA GGGGCTGCAG 
CAGCAGCCTT CAACTCCAGG GCCGCTGTCA AGCCACGCCT CCTTCCCACA GCAGCACCTA 
GCGGGCGGCC AGCCCTTCCA TGGTGTACAG CAGCCTCTTG CTCAGACAGG CATGCCGCCG 
TCCTTCTCAA AGCCCAACAC TGAAGGGGCT CCAGGAGCTC CCATTGGAAA CACCATCCAG 
CACGTGCAGG CTCTGCCAAC AGAAAAGATT ACTAAGAAAC CTATTCCGGA GGAGCACCTC 
ATCCTAAAGA CGACATTTGA GGACCTTATC CAGCGCTGCC TCTCCTCCGC TACAGACCCC 
CAAACCAAGA GGAAGCTGGA CGACGCCAGC AAGCGCCTGG AGTTCCTCTA CGACAAGCTT 
AGGGAACAGA CACTCTCCCC GACCATCATC AACGGTCTCC ACAGCATCGC CAGGAGCATT 
GAGACGAGGA ACTACTCGGA AGGGTTGAGC GTCCACACTC ACATCGTCAG CACCAGCAAC 
TTCAGTGAGA CCTCGGCCTT CATGCCCGTC CTCAAGGTTG TCCTCAGTCA GGCCAGCAAG 
CTGGGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249561.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247863.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X16, mRNA.

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CloneID	OMu70393
Clone ID Related Accession (Same CDS sequence)	XM_011249561.2 , XM_011249561.3
Accession Version	XM_011249561.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCGTCC TCAGAATGGT TGGAATGATC CTCCAGCTCT GAACAGAGTG 
CCGAAGAAGA AGAAGATGCC TGAAAACTTC ATGCCTCCTG TTCCCATTAC GTCACCAATC 
ATGAACCCTT CGGGTGATCC CCAGTCACAG GGGCTGCAGC AGCAGCCTTC AACTCCAGGG 
CCGCTGTCAA GCCACGCCTC CTTCCCACAG CAGCACCTAG CGGGCGGCCA GCCCTTCCAT 
GGTGTACAGC AGCCTCTTGC TCAGACAGGC ATGCCGCCGT CCTTCTCAAA GCCCAACACT 
GAAGGGGCTC CAGGAGCTCC CATTGGAAAC ACCATCCAGC ACGTGCAGGC TCTGCCAACA 
GAAAAGATTA CTAAGAAACC TATTCCGGAG GAGCACCTCA TCCTAAAGAC GACATTTGAG 
GACCTTATCC AGCGCTGCCT CTCCTCCGCT ACAGACCCCC AAACCAAGAG GAAGCTGGAC 
GACGCCAGCA AGCGCCTGGA GTTCCTCTAC GACAAGCTTA GGGAACAGAC ACTCTCCCCG 
ACCATCATCA ACGGTCTCCA CAGCATCGCC AGGAGCATTG AGACGAGGAA CTACTCGGAA 
GGGTTGAGCG TCCACACTCA CATCGTCAGC ACCAGCAACT TCAGTGAGAC CTCGGCCTTC 
ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247863.1
CDS	152..3562
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHVRIAPTVTTWSNKTPTALPSHPPAASPSDTQGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQRPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249561.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249561.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG TTAGAATTGC CCCTACTGTC 
ACTACCTGGA GTAACAAAAC TCCCACTGCC CTCCCCAGCC ATCCACCTGC AGCCTCTCCC 
TCTGACACAC AGGGAGAAAA TCCCCCACCT CCAGGTTTCA TAATGCAGGG CAATGTCATT 
CCAAACCCTG CTGCCCCGCT GCCCACGGCT CCAGGCCACA TGCCCAGCCA GCTGCCACCT 
TACCCACAGC CGCAGCGTCC TCAGAATGGT TGGAATGATC CTCCAGCTCT GAACAGAGTG 
CCGAAGAAGA AGAAGATGCC TGAAAACTTC ATGCCTCCTG TTCCCATTAC GTCACCAATC 
ATGAACCCTT CGGGTGATCC CCAGTCACAG GGGCTGCAGC AGCAGCCTTC AACTCCAGGG 
CCGCTGTCAA GCCACGCCTC CTTCCCACAG CAGCACCTAG CGGGCGGCCA GCCCTTCCAT 
GGTGTACAGC AGCCTCTTGC TCAGACAGGC ATGCCGCCGT CCTTCTCAAA GCCCAACACT 
GAAGGGGCTC CAGGAGCTCC CATTGGAAAC ACCATCCAGC ACGTGCAGGC TCTGCCAACA 
GAAAAGATTA CTAAGAAACC TATTCCGGAG GAGCACCTCA TCCTAAAGAC GACATTTGAG 
GACCTTATCC AGCGCTGCCT CTCCTCCGCT ACAGACCCCC AAACCAAGAG GAAGCTGGAC 
GACGCCAGCA AGCGCCTGGA GTTCCTCTAC GACAAGCTTA GGGAACAGAC ACTCTCCCCG 
ACCATCATCA ACGGTCTCCA CAGCATCGCC AGGAGCATTG AGACGAGGAA CTACTCGGAA 
GGGTTGAGCG TCCACACTCA CATCGTCAGC ACCAGCAACT TCAGTGAGAC CTCGGCCTTC 
ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249562.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247864.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X17, mRNA.

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CloneID	OMu70394
Clone ID Related Accession (Same CDS sequence)	XM_011249562.2 , XM_011249562.3
Accession Version	XM_011249562.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCGTCCTCA GAATGGTTGG 
AATGATCCTC CAGCTCTGAA CAGAGTGCCG AAGAAGAAGA AGATGCCTGA AAACTTCATG 
CCTCCTGTTC CCATTACGTC ACCAATCATG AACCCTTCGG GTGATCCCCA GTCACAGGGG 
CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG CTGTCAAGCC ACGCCTCCTT CCCACAGCAG 
CACCTAGCGG GCGGCCAGCC CTTCCATGGT GTACAGCAGC CTCTTGCTCA GACAGGCATG 
CCGCCGTCCT TCTCAAAGCC CAACACTGAA GGGGCTCCAG GAGCTCCCAT TGGAAACACC 
ATCCAGCACG TGCAGGCTCT GCCAACAGAA AAGATTACTA AGAAACCTAT TCCGGAGGAG 
CACCTCATCC TAAAGACGAC ATTTGAGGAC CTTATCCAGC GCTGCCTCTC CTCCGCTACA 
GACCCCCAAA CCAAGAGGAA GCTGGACGAC GCCAGCAAGC GCCTGGAGTT CCTCTACGAC 
AAGCTTAGGG AACAGACACT CTCCCCGACC ATCATCAACG GTCTCCACAG CATCGCCAGG 
AGCATTGAGA CGAGGAACTA CTCGGAAGGG TTGAGCGTCC ACACTCACAT CGTCAGCACC 
AGCAACTTCA GTGAGACCTC GGCCTTCATG CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC 
AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247864.1
CDS	151..3468
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKIALALNKVDGPDVALKDSDQVAQSDGEESPAAEEQLLGERIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQRPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249562.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249562.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGATTG CTTTGGCCTT GAACAAAGTG GACGGACCCG ATGTGGCTCT TAAGGACTCT 
GACCAAGTAG CGCAGAGTGA TGGGGAGGAG AGTCCTGCTG CTGAAGAGCA GCTCTTGGGA 
GAGCGCATTA AAGAGGAAAA ACAGGAATGT GACTTTCTGC CCTCGGCGGG AGGGACGTTT 
AACATCTCTG TCAGTGGGGA TATTGACGGT CTAATTACCC GGGCCTTGCT GACAGGCAAT 
TTTGAGAGTG CAGTTGACCT TTGCTTACAT GATAACCGAA TGGCTGATGC CATTATATTA 
GCCATAGCAG GTGGACAGGA ACTCTTGGCT CAAACACAAA AGAAATACTT CGCAAAATCC 
CAAAGCAAAA TTACCAGGCT TATCACTGCA GTGGTGATGA AGAACTGGAG GGAGATTGTT 
GAATCCTGTG ATCTTAAGAA TTGGAGAGAG GCTTTAGCTG CAGTCCTCAC TTACGCTAAA 
CCAGACGAGT TCTCGGCCCT GTGCGATCTC CTGGGAACCC GTCTGGAAAG AGAAGGAGAC 
AGCCTCCTGC GGACTCAGGC CTGCCTCTGC TACATCTGTG CAGGGAATGT AGAGAGATTG 
GTTGCTTGCT GGACAAAAGC TCAGGACGGC AGCAGCCCCT TGTCTCTCCA GGATCTGATT 
GAGAAAGTTG TCATCCTCCG CAAAGCTGTA CAGCTCACCC AAGCCTTGGA CACCAATACT 
GTCGGGGCTC TTCTGGCTGA GAAGATGAGT CAGTATGCCA GCTTGTTGGC AGCCCAGGGC 
AGCATTGCCG CAGCCTTGGC TTTTCTGCCT GACAACACCA ACCAGCCAAA TATTGTGCAG 
CTGCGTGACA GACTCTGTAA AGCTCAAGGC AAGCCTGTGT CGGGACAGGA ATCTTCTCAG 
AGCCCATATG AGAGGCAGCC ACTGTCCAAG GGCAGGCCTG GACCAGTTGC TGGCCACTCG 
CAGATGCCCA GAGTTCAAAC TCAGCAATAT TATCCCCATG GAGAAAATCC CCCACCTCCA 
GGTTTCATAA TGCAGGGCAA TGTCATTCCA AACCCTGCTG CCCCGCTGCC CACGGCTCCA 
GGCCACATGC CCAGCCAGCT GCCACCTTAC CCACAGCCGC AGCGTCCTCA GAATGGTTGG 
AATGATCCTC CAGCTCTGAA CAGAGTGCCG AAGAAGAAGA AGATGCCTGA AAACTTCATG 
CCTCCTGTTC CCATTACGTC ACCAATCATG AACCCTTCGG GTGATCCCCA GTCACAGGGG 
CTGCAGCAGC AGCCTTCAAC TCCAGGGCCG CTGTCAAGCC ACGCCTCCTT CCCACAGCAG 
CACCTAGCGG GCGGCCAGCC CTTCCATGGT GTACAGCAGC CTCTTGCTCA GACAGGCATG 
CCGCCGTCCT TCTCAAAGCC CAACACTGAA GGGGCTCCAG GAGCTCCCAT TGGAAACACC 
ATCCAGCACG TGCAGGCTCT GCCAACAGAA AAGATTACTA AGAAACCTAT TCCGGAGGAG 
CACCTCATCC TAAAGACGAC ATTTGAGGAC CTTATCCAGC GCTGCCTCTC CTCCGCTACA 
GACCCCCAAA CCAAGAGGAA GCTGGACGAC GCCAGCAAGC GCCTGGAGTT CCTCTACGAC 
AAGCTTAGGG AACAGACACT CTCCCCGACC ATCATCAACG GTCTCCACAG CATCGCCAGG 
AGCATTGAGA CGAGGAACTA CTCGGAAGGG TTGAGCGTCC ACACTCACAT CGTCAGCACC 
AGCAACTTCA GTGAGACCTC GGCCTTCATG CCCGTCCTCA AGGTTGTCCT CAGTCAGGCC 
AGCAAGCTGG GTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31a ( XM_011249563.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247865.1 Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu70395
Clone ID Related Accession (Same CDS sequence)	XM_011249563.2 , XM_011249563.3
Accession Version	XM_011249563.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein transport protein Sec31A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247865.1
CDS	151..3351
Translation	MKLKEIDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPHKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQKDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAVAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSIDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQSAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFESVAVPLQQGAEQQRRQPVFISQVVTEKDFLNRSAQLQHAVQSQGFIGYCQKKIEASQTEFEKNVWSFLKVNFEEDSRGKYLELLGYRKEDLGQKRIKEEKQECDFLPSAGGTFNISVSGDIDGLITRALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLAQTQKKYFAKSQSKITRLITAVVMKNWREIVESCDLKNWREALAAVLTYAKPDEFSALCDLLGTRLEREGDSLLRTQACLCYICAGNVERLVACWTKAQDGSSPLSLQDLIEKVVILRKAVQLTQALDTNTVGALLAEKMSQYASLLAAQGSIAAALAFLPDNTNQPNIVQLRDRLCKAQGKPVSGQESSQSPYERQPLSKGRPGPVAGHSQMPRVQTQQYYPHGENPPPPGFIMQGNVIPNPAAPLPTAPGHMPSQLPPYPQPQRPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPSGDPQSQGLQQQPSTPGPLSSHASFPQQHLAGGQPFHGVQQPLAQTGMPPSFSKPNTEGAPGAPIGNTIQHVQALPTEKITKKPIPEEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTIINGLHSIARSIETRNYSEGLSVHTHIVSTSNFSETSAFMPVLKVVLSQASKLGV

Target ORF information:

RefSeq Version	XM_011249563.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sec31 homolog A (S. cerevisiae) (Sec31a), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249563.2

ORF Insert Sequence:

ATGAAGTTAA AGGAGATAGA TCGTACAGCT ATGCAGGCAT GGAGCCCTGC CCAGAACCAC 
CCCATTTACC TAGCCACAGG AACATCCGCT CAGCAGTTGG ATGCGACGTT TAGTACCAAT 
GCTTCCCTCG AGATATTCGA GCTGGACCTT TCGGACCCAT CCTTGGATAT GAAGTCTTGT 
GCTACGTTCT CCTCTTCTCA CAGGTACCAC AAGTTGATCT GGGGACCTCA TAAGATGGAT 
TCCAAAGGAG ATGTTTCTGG AGTTCTGATT GCAGGTGGAG AAAATGGAAA CATTATTCTT 
TATGATCCTT CTAAAATTAT AGCTGGAGAC AAGGAAGTTG TGATTGCCCA AAAGGACAAG 
CATACTGGGC CAGTGAGAGC CTTGGATGTG AACATCTTCC AGACCAATTT GGTGGCCTCT 
GGTGCTAATG AATCTGAAAT CTACATTTGG GATCTAAACA ATTTTGCAAC TCCAATGACG 
CCGGGAGCTA AAACACAGCC ACCAGAAGAT ATCAGCTGCA TAGCATGGAA CAGACAAGTT 
CAGCATATCT TAGCATCAGC CAGTCCCAGT GGCCGGGCAA CTGTCTGGGA TCTCAGGAAA 
AACGAGCCTA TCATCAAAGT CAGTGACCAC AGTAACAGGA TGCATTGCTC TGGGCTGGCG 
TGGCACCCTG ATGTGGCTAC TCAGATGGTC CTCGCCTCTG AGGATGATCG ATTACCAGTG 
ATCCAGATGT GGGACCTTCG ATTTGCCTCC TCTCCTCTCC GGGTTCTGGA AAATCATGCC 
AGGGGGATTT TGGCAGTTGC TTGGAGCATG GCAGATCCTG AGTTGTTGTT AAGCTGTGGA 
AAAGATGCTA AGATTCTCTG CTCTAACCCT AACACAGGAG AGGTATTATA TGAACTTCCC 
ACCAACACAC AGTGGTGCTT TGATATCCAG TGGTGTCCTC GAAATCCTGC TGTCCTGTCC 
GCTGCTTCCT TTGATGGACG GATCAGTGTC TATTCCATCA TGGGAGGGAG CATAGACGGC 
TTACGGCAGA AACAAGTTGA CAAGCTTTCA TCATCTTTTG GGAATCTTGA TCCTTTCGGC 
ACAGGACAGC CTCTCCCTCC ATTACAAATC CCACAGCAGA GCGCTCAGCA CAGCATAGTG 
CTGCCCCTGA AGAAGCCTCC CAAGTGGATC CGGAGGCCTG TGGGCGCTTC CTTCTCGTTT 
GGAGGGAAGT TGGTGACTTT TGAGAGCGTT GCAGTGCCGC TGCAGCAGGG AGCTGAGCAG 
CAGCGACGGC AGCCTGTGTT CATCAGTCAG GTGGTGACGG AGAAGGACTT TCTCAACCGG 
TCGGCGCAGC TGCAGCATGC TGTGCAGTCT CAGGGCTTCA TCGGTTACTG CCAGAAAAAG 
ATCGAGGCTT CCCAGACCGA GTTTGAGAAA AATGTGTGGT CCTTCTTGAA GGTAAACTTT 
GAGGAGGATT CTCGTGGAAA ATACCTTGAG CTTCTGGGAT ACAGAAAGGA AGATCTGGGG 
CAGAAGCGCA TTAAAGAGGA AAAACAGGAA TGTGACTTTC TGCCCTCGGC GGGAGGGACG 
TTTAACATCT CTGTCAGTGG GGATATTGAC GGTCTAATTA CCCGGGCCTT GCTGACAGGC 
AATTTTGAGA GTGCAGTTGA CCTTTGCTTA CATGATAACC GAATGGCTGA TGCCATTATA 
TTAGCCATAG CAGGTGGACA GGAACTCTTG GCTCAAACAC AAAAGAAATA CTTCGCAAAA 
TCCCAAAGCA AAATTACCAG GCTTATCACT GCAGTGGTGA TGAAGAACTG GAGGGAGATT 
GTTGAATCCT GTGATCTTAA GAATTGGAGA GAGGCTTTAG CTGCAGTCCT CACTTACGCT 
AAACCAGACG AGTTCTCGGC CCTGTGCGAT CTCCTGGGAA CCCGTCTGGA AAGAGAAGGA 
GACAGCCTCC TGCGGACTCA GGCCTGCCTC TGCTACATCT GTGCAGGGAA TGTAGAGAGA 
TTGGTTGCTT GCTGGACAAA AGCTCAGGAC GGCAGCAGCC CCTTGTCTCT CCAGGATCTG 
ATTGAGAAAG TTGTCATCCT CCGCAAAGCT GTACAGCTCA CCCAAGCCTT GGACACCAAT 
ACTGTCGGGG CTCTTCTGGC TGAGAAGATG AGTCAGTATG CCAGCTTGTT GGCAGCCCAG 
GGCAGCATTG CCGCAGCCTT GGCTTTTCTG CCTGACAACA CCAACCAGCC AAATATTGTG 
CAGCTGCGTG ACAGACTCTG TAAAGCTCAA GGCAAGCCTG TGTCGGGACA GGAATCTTCT 
CAGAGCCCAT ATGAGAGGCA GCCACTGTCC AAGGGCAGGC CTGGACCAGT TGCTGGCCAC 
TCGCAGATGC CCAGAGTTCA AACTCAGCAA TATTATCCCC ATGGAGAAAA TCCCCCACCT 
CCAGGTTTCA TAATGCAGGG CAATGTCATT CCAAACCCTG CTGCCCCGCT GCCCACGGCT 
CCAGGCCACA TGCCCAGCCA GCTGCCACCT TACCCACAGC CGCAGCGTCC TCAGAATGGT 
TGGAATGATC CTCCAGCTCT GAACAGAGTG CCGAAGAAGA AGAAGATGCC TGAAAACTTC 
ATGCCTCCTG TTCCCATTAC GTCACCAATC ATGAACCCTT CGGGTGATCC CCAGTCACAG 
GGGCTGCAGC AGCAGCCTTC AACTCCAGGG CCGCTGTCAA GCCACGCCTC CTTCCCACAG 
CAGCACCTAG CGGGCGGCCA GCCCTTCCAT GGTGTACAGC AGCCTCTTGC TCAGACAGGC 
ATGCCGCCGT CCTTCTCAAA GCCCAACACT GAAGGGGCTC CAGGAGCTCC CATTGGAAAC 
ACCATCCAGC ACGTGCAGGC TCTGCCAACA GAAAAGATTA CTAAGAAACC TATTCCGGAG 
GAGCACCTCA TCCTAAAGAC GACATTTGAG GACCTTATCC AGCGCTGCCT CTCCTCCGCT 
ACAGACCCCC AAACCAAGAG GAAGCTGGAC GACGCCAGCA AGCGCCTGGA GTTCCTCTAC 
GACAAGCTTA GGGAACAGAC ACTCTCCCCG ACCATCATCA ACGGTCTCCA CAGCATCGCC 
AGGAGCATTG AGACGAGGAA CTACTCGGAA GGGTTGAGCG TCCACACTCA CATCGTCAGC 
ACCAGCAACT TCAGTGAGAC CTCGGCCTTC ATGCCCGTCC TCAAGGTTGT CCTCAGTCAG 
GCCAGCAAGC TGGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FGF signalling regulates bone growth through autophagy.
Nature528(7581)272-5(2015 Dec)
Cinque L,Forrester A,Bartolomeo R,Svelto M,Venditti R,Montefusco S,Polishchuk E,Nusco E,Rossi A,Medina DL,Polishchuk R,De Matteis MA,Settembre C

Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to allow ER-Golgi export.
The EMBO journal33(20)2314-31(2014 Oct)
Cho HJ,Yu J,Xie C,Rudrabhatla P,Chen X,Wu J,Parisiadou L,Liu G,Sun L,Ma B,Ding J,Liu Z,Cai H

Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans.
American journal of human genetics93(6)1001-14(2013 Dec)
Liegel RP,Handley MT,Ronchetti A,Brown S,Langemeyer L,Linford A,Chang B,Morris-Rosendahl DJ,Carpanini S,Posmyk R,Harthill V,Sheridan E,Abdel-Salam GM,Terhal PA,Faravelli F,Accorsi P,Giordano L,Pinelli L,Hartmann B,Ebert AD,Barr FA,Aligianis IA,Sidjanin DJ

The E3-ubiquitin ligase TRIM50 interacts with HDAC6 and p62, and promotes the sequestration and clearance of ubiquitinated proteins into the aggresome.
PloS one7(7)e40440(2012)
Fusco C,Micale L,Egorov M,Monti M,D'Addetta EV,Augello B,Cozzolino F,Calcagn? A,Fontana A,Polishchuk RS,Didelot G,Reymond A,Pucci P,Merla G

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Sec31a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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