Sec31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec31
Entrez Gene ID	35877
Full Name	Secretory 31
Synonyms	CG8266,Dmel\CG8266,SEC31,dSec31p,sec31
General protein information	Preferred Names Secretory 31 Names CG8266 gene product from transcript CG8266-RD CG8266-PA CG8266-PB CG8266-PC CG8266-PD Sec31 ortholog Sec31-PA Sec31-PB Sec31-PC Sec31-PD sec31 secretory 31
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44F3-44F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165630.3	NP_724716.1	secretory 31, isoform B
NM_136570.4	NP_610414.1	secretory 31, isoform A
NM_001299285.1	NP_001286214.1	secretory 31, isoform C
NM_001299286.1	NP_001286215.1	secretory 31, isoform D

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sec31a	NP_955824.1
Canis lupus familiaris (dog)	SEC31A	XP_005639183.1
Mus musculus (house mouse)	Sec31a	NP_081245.1
Gallus gallus (chicken)	SEC31A	NP_001026312.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011551	XP_309957.4
Rattus norvegicus (Norway rat)	Sec31a	NP_148981.1
Homo sapiens (human)	SEC31A	NP_001070676.1
Drosophila melanogaster (fruit fly)	sec31	NP_724716.1
Pan troglodytes (chimpanzee)	SEC31A	XP_001140439.1
	SEC31A	XP_001085944.1
Bos taurus (cattle)	SEC31A	NP_001178203.2
Xenopus tropicalis (tropical clawed frog)	sec31a	NP_001011158.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sec31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12810	NM_165630.3 Latest version!	Drosophila melanogaster secretory 31, transcript variant B (Sec31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa12811	NM_136570.4 Latest version!	Drosophila melanogaster secretory 31, transcript variant A (Sec31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21721	NM_001299286.1 Latest version!	Drosophila melanogaster secretory 31, transcript variant D (Sec31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21722	NM_001299285.1 Latest version!	Drosophila melanogaster secretory 31, transcript variant C (Sec31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec31 ( NM_165630.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724716.1 Drosophila melanogaster secretory 31, transcript variant B (Sec31), mRNA.

Price & Availability↑

CloneID	OFa12810
Clone ID Related Accession (Same CDS sequence)	NM_165630.3
Accession Version	NM_165630.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 31, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165630.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATCA GACGCAAGAT AACAAGTTTG ATGGCAATGC AATCGACTCA 
CAAAATCACA ACGACTTTGC CACTCCTCCG AGGCCGCAAT TCAAGGTTCC CAGCGGAGAC 
CACTCGGATA GCCTGATTGC CGAGGCAATA CTCACTGGCA ATGTGGACGC TGCCGTGGAG 
CTCTGTCTGG AAGCACAGCG CATTCCTGAA GCCCTGATTA TCGCCTCAAC GGCTGGCATT 
GAGACTCTGA ACCGCACGCA AACGCGCTAC CTGCTGCAGC AGAAGAACGA GCTATCGCAC 
GTTATCTCCG CTTTGGTATC GCGCGACTGG TTGGACTTCG TCAACAGATG CACTGTGGAC 
TCGTGGAAGG AGGCCCTGGT GGCCGCGCTC AAGCACAGCG AGCGCAAGGT GGTGGACATC 
TGCGAGCGAC TAGGCGATCG CCTCTTAAGT GAGTGCGCCT CAAGCGCCGA GTTTACGCGC 
AACGCCATGC TCTGCTACAT TTGCGCCGGC AGCATCGACA AACTGGTCAC CGCGTGGTAC 
CAGTTAAAGC GGCTGGAGCA GCAGAACCCC GGCTACAAGC CCAACACCAC AGAGCTGCAG 
GACTTGGCTG AGATGGTGAT GCTGATGTGC AAGTCGTTGG AGCAGCAAGG CATATCCGTG 
GACTTGGCTG GTCGCTTTGC CGGCTTTCTG ACTGAGTACG GCGGTCTTCT TGCGTCCCAG 
GGTGCTCTGA CTGCAGCCCT GCAGTACATT ACCACCTTGG GCGGAGGAGC TGCCGCCAGC 
GAAAGCGATG TTCTGCAGAG CCTACGAGAC CGGATAAATA ACGCTGTCAA CCTGGACTAC 
ACCCAGCCAC AGGCAGCAGT CGCTGCTGCG CCACACGGCT ACCAACCGAC TCGTGGTCGT 
GGATCGTTCT CGCAGCCACA GCCTTCGGCT CCCATGCCCT ACGGCCAGCC GCCCAATCAG 
TATGCACAGC CCAGCTGGCC AGCGCAAGTG CCTCCACTAC AAACAAGCTA CCCAGGAGCC 
CAGCAGCCTC AGGCTCCGCC GCAGATATTC AATCCGCAGC CTGTGAAACC AGAGCCCCTG 
GCCCAACCAC CGCGCCCACT CAGCAATGCC AGCTCTTCAG GTGGTCCGCC GCCAGCAGGC 
GGCGCATCAT CCTCCGGCGG ATCGGGTCTT TCTGGCCGCT CCAAATACGT TTTGGACCCA 
TCTGTAGCAG CTCCAACCAG TTCCTACGGA ATGCCATACA ATCCAGTGCC GGCCCCCGTT 
CCATCAGCGG TTCCATTTGG CGGCGGAGGA GTGCCTGGGC CCGCACCCCA GCCAGCTAGC 
GTGCCAACGT ACAACAGCAA CGCGTTCAAC ACGAATCCTA TTGCGGCTGC CCAACCTTAC 
AACCCTTCGC CACTCATGCC AGCTCAGCAG AATCAGTTCG TGCCCGGTGT GAATCCCATC 
GAGACAATGC CACCGCCGGC TGTGCCACCA GTCATCCAGA ATGTGCAACG CAATCCCACA 
CCACCACCTG GCTGGAACGA TCCTCCTGCA CTGAAATCTT CGCGAGCGCC TAAGCCGAAG 
CCGGTGGTTG AGCCAAGTGC TGCTATCTTC CATCCCCTGT TCCCAGTCGA CCCGAATCAG 
AACCAGAACG GTTATGTGGA CCCCTCTCAA TATCAGCACG CAGCACCACT TGGAGGAGTG 
CCCAATACGT ACTACAACCC GGCTGCCTTC AACAACAATC CCCAGCAGCA GCAGCAGCAT 
CAGCAATCAT ATCTCCAGCC TCAGCAGCCA GCCCCTGGGA TTCAGCAGCC AAACTGGCAG 
GGACAACAGC AAACCATTGG CTATACCGAG CAGCCAGCGC CCATCCAGCA ACAGCGCCAA 
CCGGCGCCGC CCAAGGAGAA GCCACCACTC CCCGAGGAGT ACATCTACTT GCAGACTGTG 
CTGGAGGAGC TGAAGAACCG CTGCCTGGGC GCAACCAATG ATCCGCGCAC AAAACGAAAG 
TTTGTCGATG TTGTCAAGAG GCTCGAAAAT CTCTACGACT GCCTGAGAGA TGGAAAGCTC 
ACTCCCACCA CGGTGCAATA TCTGAATCAA ATAGTTCAGT ACATTCAAAT ATCCGACTAT 
GCCAATGCTA TGGAAATCCA CACGCAAATC GCCTTCGGAA CGGACTTTGC GCAGTGCGCG 
GGCTTTATGC AGGGACTGAA GGTCCTACTG CAATCCGCAT CCGAGCTACA GTTGGTTCTG 
CGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724716.1
CDS	145..3870
Translation	MKIKELQKTVNIAWSPAQQQQILLAAGTAAQQFDSNANSTLELYSPNFSDATYDLELRASVSSQYKFQKLIWSPVGPHPSGLIVGGCEAGQINIYSADKLLAGKEEPLLKRQDKHTGAVSGLDFNPFQNNLLASCASESEILIWDLNNPETSMSPGTKTQPLEDVKNVAWNRQVQHILASVFSTRCVIWDLRKSQQIIKLSDSQSRVRWHAIEWHPEAATQVWLASEDDQAPVVQLWDLRYATAPAKTYQIHERGVLGMSWCLQDNDLMVSCGKDNRIYCWNPNTKIPEGEILSEVATTASWYSDVQFCPRNPALIASASLEGAVSIYSLHGGTHHLVQTANKIADSFPGMDQFAQEPIPQQATQVVYHDLAHAPKWMKRPCGVAFGFGGKLVSFNGTSKTVKVQQLTTESALVDRANALERSLVDANYSDYCRQRADETPDQHGRYIWYFIKANFELNPKEEMLNLLGYNKDDIDSKFNKFIKETDGNSHSDVDTLTTRISTLTHQTQDNKFDGNAIDSQNHNDFATPPRPQFKVPSGDHSDSLIAEAILTGNVDAAVELCLEAQRIPEALIIASTAGIETLNRTQTRYLLQQKNELSHVISALVSRDWLDFVNRCTVDSWKEALVAALKHSERKVVDICERLGDRLLSECASSAEFTRNAMLCYICAGSIDKLVTAWYQLKRLEQQNPGYKPNTTELQDLAEMVMLMCKSLEQQGISVDLAGRFAGFLTEYGGLLASQGALTAALQYITTLGGGAAASESDVLQSLRDRINNAVNLDYTQPQAAVAAAPHGYQPTRGRGSFSQPQPSAPMPYGQPPNQYAQPSWPAQVPPLQTSYPGAQQPQAPPQIFNPQPVKPEPLAQPPRPLSNASSSGGPPPAGGASSSGGSGLSGRSKYVLDPSVAAPTSSYGMPYNPVPAPVPSAVPFGGGGVPGPAPQPASVPTYNSNAFNTNPIAAAQPYNPSPLMPAQQNQFVPGVNPIETMPPPAVPPVIQNVQRNPTPPPGWNDPPALKSSRAPKPKPVVEPSAAIFHPLFPVDPNQNQNGYVDPSQYQHAAPLGGVPNTYYNPAAFNNNPQQQQQHQQSYLQPQQPAPGIQQPNWQGQQQTIGYTEQPAPIQQQRQPAPPKEKPPLPEEYIYLQTVLEELKNRCLGATNDPRTKRKFVDVVKRLENLYDCLRDGKLTPTTVQYLNQIVQYIQISDYANAMEIHTQIAFGTDFAQCAGFMQGLKVLLQSASELQLVLR

Target ORF information:

RefSeq Version	NM_165630.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 31, transcript variant B (Sec31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165630.3

ORF Insert Sequence:

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATCA GACGCAAGAT AACAAGTTTG ATGGCAATGC AATCGACTCA 
CAAAATCACA ACGACTTTGC CACTCCTCCG AGGCCGCAAT TCAAGGTTCC CAGCGGAGAC 
CACTCGGATA GCCTGATTGC CGAGGCAATA CTCACTGGCA ATGTGGACGC TGCCGTGGAG 
CTCTGTCTGG AAGCACAGCG CATTCCTGAA GCCCTGATTA TCGCCTCAAC GGCTGGCATT 
GAGACTCTGA ACCGCACGCA AACGCGCTAC CTGCTGCAGC AGAAGAACGA GCTATCGCAC 
GTTATCTCCG CTTTGGTATC GCGCGACTGG TTGGACTTCG TCAACAGATG CACTGTGGAC 
TCGTGGAAGG AGGCCCTGGT GGCCGCGCTC AAGCACAGCG AGCGCAAGGT GGTGGACATC 
TGCGAGCGAC TAGGCGATCG CCTCTTAAGT GAGTGCGCCT CAAGCGCCGA GTTTACGCGC 
AACGCCATGC TCTGCTACAT TTGCGCCGGC AGCATCGACA AACTGGTCAC CGCGTGGTAC 
CAGTTAAAGC GGCTGGAGCA GCAGAACCCC GGCTACAAGC CCAACACCAC AGAGCTGCAG 
GACTTGGCTG AGATGGTGAT GCTGATGTGC AAGTCGTTGG AGCAGCAAGG CATATCCGTG 
GACTTGGCTG GTCGCTTTGC CGGCTTTCTG ACTGAGTACG GCGGTCTTCT TGCGTCCCAG 
GGTGCTCTGA CTGCAGCCCT GCAGTACATT ACCACCTTGG GCGGAGGAGC TGCCGCCAGC 
GAAAGCGATG TTCTGCAGAG CCTACGAGAC CGGATAAATA ACGCTGTCAA CCTGGACTAC 
ACCCAGCCAC AGGCAGCAGT CGCTGCTGCG CCACACGGCT ACCAACCGAC TCGTGGTCGT 
GGATCGTTCT CGCAGCCACA GCCTTCGGCT CCCATGCCCT ACGGCCAGCC GCCCAATCAG 
TATGCACAGC CCAGCTGGCC AGCGCAAGTG CCTCCACTAC AAACAAGCTA CCCAGGAGCC 
CAGCAGCCTC AGGCTCCGCC GCAGATATTC AATCCGCAGC CTGTGAAACC AGAGCCCCTG 
GCCCAACCAC CGCGCCCACT CAGCAATGCC AGCTCTTCAG GTGGTCCGCC GCCAGCAGGC 
GGCGCATCAT CCTCCGGCGG ATCGGGTCTT TCTGGCCGCT CCAAATACGT TTTGGACCCA 
TCTGTAGCAG CTCCAACCAG TTCCTACGGA ATGCCATACA ATCCAGTGCC GGCCCCCGTT 
CCATCAGCGG TTCCATTTGG CGGCGGAGGA GTGCCTGGGC CCGCACCCCA GCCAGCTAGC 
GTGCCAACGT ACAACAGCAA CGCGTTCAAC ACGAATCCTA TTGCGGCTGC CCAACCTTAC 
AACCCTTCGC CACTCATGCC AGCTCAGCAG AATCAGTTCG TGCCCGGTGT GAATCCCATC 
GAGACAATGC CACCGCCGGC TGTGCCACCA GTCATCCAGA ATGTGCAACG CAATCCCACA 
CCACCACCTG GCTGGAACGA TCCTCCTGCA CTGAAATCTT CGCGAGCGCC TAAGCCGAAG 
CCGGTGGTTG AGCCAAGTGC TGCTATCTTC CATCCCCTGT TCCCAGTCGA CCCGAATCAG 
AACCAGAACG GTTATGTGGA CCCCTCTCAA TATCAGCACG CAGCACCACT TGGAGGAGTG 
CCCAATACGT ACTACAACCC GGCTGCCTTC AACAACAATC CCCAGCAGCA GCAGCAGCAT 
CAGCAATCAT ATCTCCAGCC TCAGCAGCCA GCCCCTGGGA TTCAGCAGCC AAACTGGCAG 
GGACAACAGC AAACCATTGG CTATACCGAG CAGCCAGCGC CCATCCAGCA ACAGCGCCAA 
CCGGCGCCGC CCAAGGAGAA GCCACCACTC CCCGAGGAGT ACATCTACTT GCAGACTGTG 
CTGGAGGAGC TGAAGAACCG CTGCCTGGGC GCAACCAATG ATCCGCGCAC AAAACGAAAG 
TTTGTCGATG TTGTCAAGAG GCTCGAAAAT CTCTACGACT GCCTGAGAGA TGGAAAGCTC 
ACTCCCACCA CGGTGCAATA TCTGAATCAA ATAGTTCAGT ACATTCAAAT ATCCGACTAT 
GCCAATGCTA TGGAAATCCA CACGCAAATC GCCTTCGGAA CGGACTTTGC GCAGTGCGCG 
GGCTTTATGC AGGGACTGAA GGTCCTACTG CAATCCGCAT CCGAGCTACA GTTGGTTCTG 
CGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31 ( NM_136570.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610414.1 Drosophila melanogaster secretory 31, transcript variant A (Sec31), mRNA.

Price & Availability↑

CloneID	OFa12811
Clone ID Related Accession (Same CDS sequence)	NM_136570.4
Accession Version	NM_136570.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 31, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136570.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATAC GCAAGATAAC AAGTTTGATG GCAATGCAAT CGACTCACAA 
AATCACAACG ACTTTGCCAC TCCTCCGAGG CCGCAATTCA AGGTTCCCAG CGGAGACCAC 
TCGGATAGCC TGATTGCCGA GGCAATACTC ACTGGCAATG TGGACGCTGC CGTGGAGCTC 
TGTCTGGAAG CACAGCGCAT TCCTGAAGCC CTGATTATCG CCTCAACGGC TGGCATTGAG 
ACTCTGAACC GCACGCAAAC GCGCTACCTG CTGCAGCAGA AGAACGAGCT ATCGCACGTT 
ATCTCCGCTT TGGTATCGCG CGACTGGTTG GACTTCGTCA ACAGATGCAC TGTGGACTCG 
TGGAAGGAGG CCCTGGTGGC CGCGCTCAAG CACAGCGAGC GCAAGGTGGT GGACATCTGC 
GAGCGACTAG GCGATCGCCT CTTAAGTGAG TGCGCCTCAA GCGCCGAGTT TACGCGCAAC 
GCCATGCTCT GCTACATTTG CGCCGGCAGC ATCGACAAAC TGGTCACCGC GTGGTACCAG 
TTAAAGCGGC TGGAGCAGCA GAACCCCGGC TACAAGCCCA ACACCACAGA GCTGCAGGAC 
TTGGCTGAGA TGGTGATGCT GATGTGCAAG TCGTTGGAGC AGCAAGGCAT ATCCGTGGAC 
TTGGCTGGTC GCTTTGCCGG CTTTCTGACT GAGTACGGCG GTCTTCTTGC GTCCCAGGGT 
GCTCTGACTG CAGCCCTGCA GTACATTACC ACCTTGGGCG GAGGAGCTGC CGCCAGCGAA 
AGCGATGTTC TGCAGAGCCT ACGAGACCGG ATAAATAACG CTGTCAACCT GGACTACACC 
CAGCCACAGG CAGCAGTCGC TGCTGCGCCA CACGGCTACC AACCGACTCG TGGTCGTGGA 
TCGTTCTCGC AGCCACAGCC TTCGGCTCCC ATGCCCTACG GCCAGCCGCC CAATCAGTAT 
GCACAGCCCA GCTGGCCAGC GCAAGTGCCT CCACTACAAA CAAGCTACCC AGGAGCCCAG 
CAGCCTCAGG CTCCGCCGCA GATATTCAAT CCGCAGCCTG TGAAACCAGA GCCCCTGGCC 
CAACCACCGC GCCCACTCAG CAATGCCAGC TCTTCAGGTG GTCCGCCGCC AGCAGGCGGC 
GCATCATCCT CCGGCGGATC GGGTCTTTCT GGCCGCTCCA AATACGTTTT GGACCCATCT 
GTAGCAGCTC CAACCAGTTC CTACGGAATG CCATACAATC CAGTGCCGGC CCCCGTTCCA 
TCAGCGGTTC CATTTGGCGG CGGAGGAGTG CCTGGGCCCG CACCCCAGCC AGCTAGCGTG 
CCAACGTACA ACAGCAACGC GTTCAACACG AATCCTATTG CGGCTGCCCA ACCTTACAAC 
CCTTCGCCAC TCATGCCAGC TCAGCAGAAT CAGTTCGTGC CCGGTGTGAA TCCCATCGAG 
ACAATGCCAC CGCCGGCTGT GCCACCAGTC ATCCAGAATG TGCAACGCAA TCCCACACCA 
CCACCTGGCT GGAACGATCC TCCTGCACTG AAATCTTCGC GAGCGCCTAA GCCGAAGCCG 
GTGGTTGAGC CAAGTGCTGC TATCTTCCAT CCCCTGTTCC CAGTCGACCC GAATCAGAAC 
CAGAACGGTT ATGTGGACCC CTCTCAATAT CAGCACGCAG CACCACTTGG AGGAGTGCCC 
AATACGTACT ACAACCCGGC TGCCTTCAAC AACAATCCCC AGCAGCAGCA GCAGCATCAG 
CAATCATATC TCCAGCCTCA GCAGCCAGCC CCTGGGATTC AGCAGCCAAA CTGGCAGGGA 
CAACAGCAAA CCATTGGCTA TACCGAGCAG CCAGCGCCCA TCCAGCAACA GCGCCAACCG 
GCGCCGCCCA AGGAGAAGCC ACCACTCCCC GAGGAGTACA TCTACTTGCA GACTGTGCTG 
GAGGAGCTGA AGAACCGCTG CCTGGGCGCA ACCAATGATC CGCGCACAAA ACGAAAGTTT 
GTCGATGTTG TCAAGAGGCT CGAAAATCTC TACGACTGCC TGAGAGATGG AAAGCTCACT 
CCCACCACGG TGCAATATCT GAATCAAATA GTTCAGTACA TTCAAATATC CGACTATGCC 
AATGCTATGG AAATCCACAC GCAAATCGCC TTCGGAACGG ACTTTGCGCA GTGCGCGGGC 
TTTATGCAGG GACTGAAGGT CCTACTGCAA TCCGCATCCG AGCTACAGTT GGTTCTGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610414.1
CDS	145..3867
Translation	MKIKELQKTVNIAWSPAQQQQILLAAGTAAQQFDSNANSTLELYSPNFSDATYDLELRASVSSQYKFQKLIWSPVGPHPSGLIVGGCEAGQINIYSADKLLAGKEEPLLKRQDKHTGAVSGLDFNPFQNNLLASCASESEILIWDLNNPETSMSPGTKTQPLEDVKNVAWNRQVQHILASVFSTRCVIWDLRKSQQIIKLSDSQSRVRWHAIEWHPEAATQVWLASEDDQAPVVQLWDLRYATAPAKTYQIHERGVLGMSWCLQDNDLMVSCGKDNRIYCWNPNTKIPEGEILSEVATTASWYSDVQFCPRNPALIASASLEGAVSIYSLHGGTHHLVQTANKIADSFPGMDQFAQEPIPQQATQVVYHDLAHAPKWMKRPCGVAFGFGGKLVSFNGTSKTVKVQQLTTESALVDRANALERSLVDANYSDYCRQRADETPDQHGRYIWYFIKANFELNPKEEMLNLLGYNKDDIDSKFNKFIKETDGNSHSDVDTLTTRISTLTHTQDNKFDGNAIDSQNHNDFATPPRPQFKVPSGDHSDSLIAEAILTGNVDAAVELCLEAQRIPEALIIASTAGIETLNRTQTRYLLQQKNELSHVISALVSRDWLDFVNRCTVDSWKEALVAALKHSERKVVDICERLGDRLLSECASSAEFTRNAMLCYICAGSIDKLVTAWYQLKRLEQQNPGYKPNTTELQDLAEMVMLMCKSLEQQGISVDLAGRFAGFLTEYGGLLASQGALTAALQYITTLGGGAAASESDVLQSLRDRINNAVNLDYTQPQAAVAAAPHGYQPTRGRGSFSQPQPSAPMPYGQPPNQYAQPSWPAQVPPLQTSYPGAQQPQAPPQIFNPQPVKPEPLAQPPRPLSNASSSGGPPPAGGASSSGGSGLSGRSKYVLDPSVAAPTSSYGMPYNPVPAPVPSAVPFGGGGVPGPAPQPASVPTYNSNAFNTNPIAAAQPYNPSPLMPAQQNQFVPGVNPIETMPPPAVPPVIQNVQRNPTPPPGWNDPPALKSSRAPKPKPVVEPSAAIFHPLFPVDPNQNQNGYVDPSQYQHAAPLGGVPNTYYNPAAFNNNPQQQQQHQQSYLQPQQPAPGIQQPNWQGQQQTIGYTEQPAPIQQQRQPAPPKEKPPLPEEYIYLQTVLEELKNRCLGATNDPRTKRKFVDVVKRLENLYDCLRDGKLTPTTVQYLNQIVQYIQISDYANAMEIHTQIAFGTDFAQCAGFMQGLKVLLQSASELQLVLR

Target ORF information:

RefSeq Version	NM_136570.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 31, transcript variant A (Sec31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136570.4

ORF Insert Sequence:

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATAC GCAAGATAAC AAGTTTGATG GCAATGCAAT CGACTCACAA 
AATCACAACG ACTTTGCCAC TCCTCCGAGG CCGCAATTCA AGGTTCCCAG CGGAGACCAC 
TCGGATAGCC TGATTGCCGA GGCAATACTC ACTGGCAATG TGGACGCTGC CGTGGAGCTC 
TGTCTGGAAG CACAGCGCAT TCCTGAAGCC CTGATTATCG CCTCAACGGC TGGCATTGAG 
ACTCTGAACC GCACGCAAAC GCGCTACCTG CTGCAGCAGA AGAACGAGCT ATCGCACGTT 
ATCTCCGCTT TGGTATCGCG CGACTGGTTG GACTTCGTCA ACAGATGCAC TGTGGACTCG 
TGGAAGGAGG CCCTGGTGGC CGCGCTCAAG CACAGCGAGC GCAAGGTGGT GGACATCTGC 
GAGCGACTAG GCGATCGCCT CTTAAGTGAG TGCGCCTCAA GCGCCGAGTT TACGCGCAAC 
GCCATGCTCT GCTACATTTG CGCCGGCAGC ATCGACAAAC TGGTCACCGC GTGGTACCAG 
TTAAAGCGGC TGGAGCAGCA GAACCCCGGC TACAAGCCCA ACACCACAGA GCTGCAGGAC 
TTGGCTGAGA TGGTGATGCT GATGTGCAAG TCGTTGGAGC AGCAAGGCAT ATCCGTGGAC 
TTGGCTGGTC GCTTTGCCGG CTTTCTGACT GAGTACGGCG GTCTTCTTGC GTCCCAGGGT 
GCTCTGACTG CAGCCCTGCA GTACATTACC ACCTTGGGCG GAGGAGCTGC CGCCAGCGAA 
AGCGATGTTC TGCAGAGCCT ACGAGACCGG ATAAATAACG CTGTCAACCT GGACTACACC 
CAGCCACAGG CAGCAGTCGC TGCTGCGCCA CACGGCTACC AACCGACTCG TGGTCGTGGA 
TCGTTCTCGC AGCCACAGCC TTCGGCTCCC ATGCCCTACG GCCAGCCGCC CAATCAGTAT 
GCACAGCCCA GCTGGCCAGC GCAAGTGCCT CCACTACAAA CAAGCTACCC AGGAGCCCAG 
CAGCCTCAGG CTCCGCCGCA GATATTCAAT CCGCAGCCTG TGAAACCAGA GCCCCTGGCC 
CAACCACCGC GCCCACTCAG CAATGCCAGC TCTTCAGGTG GTCCGCCGCC AGCAGGCGGC 
GCATCATCCT CCGGCGGATC GGGTCTTTCT GGCCGCTCCA AATACGTTTT GGACCCATCT 
GTAGCAGCTC CAACCAGTTC CTACGGAATG CCATACAATC CAGTGCCGGC CCCCGTTCCA 
TCAGCGGTTC CATTTGGCGG CGGAGGAGTG CCTGGGCCCG CACCCCAGCC AGCTAGCGTG 
CCAACGTACA ACAGCAACGC GTTCAACACG AATCCTATTG CGGCTGCCCA ACCTTACAAC 
CCTTCGCCAC TCATGCCAGC TCAGCAGAAT CAGTTCGTGC CCGGTGTGAA TCCCATCGAG 
ACAATGCCAC CGCCGGCTGT GCCACCAGTC ATCCAGAATG TGCAACGCAA TCCCACACCA 
CCACCTGGCT GGAACGATCC TCCTGCACTG AAATCTTCGC GAGCGCCTAA GCCGAAGCCG 
GTGGTTGAGC CAAGTGCTGC TATCTTCCAT CCCCTGTTCC CAGTCGACCC GAATCAGAAC 
CAGAACGGTT ATGTGGACCC CTCTCAATAT CAGCACGCAG CACCACTTGG AGGAGTGCCC 
AATACGTACT ACAACCCGGC TGCCTTCAAC AACAATCCCC AGCAGCAGCA GCAGCATCAG 
CAATCATATC TCCAGCCTCA GCAGCCAGCC CCTGGGATTC AGCAGCCAAA CTGGCAGGGA 
CAACAGCAAA CCATTGGCTA TACCGAGCAG CCAGCGCCCA TCCAGCAACA GCGCCAACCG 
GCGCCGCCCA AGGAGAAGCC ACCACTCCCC GAGGAGTACA TCTACTTGCA GACTGTGCTG 
GAGGAGCTGA AGAACCGCTG CCTGGGCGCA ACCAATGATC CGCGCACAAA ACGAAAGTTT 
GTCGATGTTG TCAAGAGGCT CGAAAATCTC TACGACTGCC TGAGAGATGG AAAGCTCACT 
CCCACCACGG TGCAATATCT GAATCAAATA GTTCAGTACA TTCAAATATC CGACTATGCC 
AATGCTATGG AAATCCACAC GCAAATCGCC TTCGGAACGG ACTTTGCGCA GTGCGCGGGC 
TTTATGCAGG GACTGAAGGT CCTACTGCAA TCCGCATCCG AGCTACAGTT GGTTCTGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31 ( NM_001299286.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286215.1 Drosophila melanogaster secretory 31, transcript variant D (Sec31), mRNA.

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CloneID	OFa21721
Clone ID Related Accession (Same CDS sequence)	NM_001299286.1
Accession Version	NM_001299286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 31, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATAG CGACTCTTCT GAGGTCGAGT GTGATCAGAG TACTAACTAT 
AGCACCGATA ATTCGCAGAC GCAAGATAAC AAGTTTGATG GCAATGCAAT CGACTCACAA 
AATCACAACG ACTTTGCCAC TCCTCCGAGG CCGCAATTCA AGGTTCCCAG CGGAGACCAC 
TCGGATAGCC TGATTGCCGA GGCAATACTC ACTGGCAATG TGGACGCTGC CGTGGAGCTC 
TGTCTGGAAG CACAGCGCAT TCCTGAAGCC CTGATTATCG CCTCAACGGC TGGCATTGAG 
ACTCTGAACC GCACGCAAAC GCGCTACCTG CTGCAGCAGA AGAACGAGCT ATCGCACGTT 
ATCTCCGCTT TGGTATCGCG CGACTGGTTG GACTTCGTCA ACAGATGCAC TGTGGACTCG 
TGGAAGGAGG CCCTGGTGGC CGCGCTCAAG CACAGCGAGC GCAAGGTGGT GGACATCTGC 
GAGCGACTAG GCGATCGCCT CTTAAGTGAG TGCGCCTCAA GCGCCGAGTT TACGCGCAAC 
GCCATGCTCT GCTACATTTG CGCCGGCAGC ATCGACAAAC TGGTCACCGC GTGGTACCAG 
TTAAAGCGGC TGGAGCAGCA GAACCCCGGC TACAAGCCCA ACACCACAGA GCTGCAGGAC 
TTGGCTGAGA TGGTGATGCT GATGTGCAAG TCGTTGGAGC AGCAAGGCAT ATCCGTGGAC 
TTGGCTGGTC GCTTTGCCGG CTTTCTGACT GAGTACGGCG GTCTTCTTGC GTCCCAGGGT 
GCTCTGACTG CAGCCCTGCA GTACATTACC ACCTTGGGCG GAGGAGCTGC CGCCAGCGAA 
AGCGATGTTC TGCAGAGCCT ACGAGACCGG ATAAATAACG CTGTCAACCT GGACTACACC 
CAGCCACAGG CAGCAGTCGC TGCTGCGCCA CACGGCTACC AACCGACTCG TGGTCGTGGA 
TCGTTCTCGC AGCCACAGCC TTCGGCTCCC ATGCCCTACG GCCAGCCGCC CAATCAGTAT 
GCACAGCCCA GCTGGCCAGC GCAAGTGCCT CCACTACAAA CAAGCTACCC AGGAGCCCAG 
CAGCCTCAGG CTCCGCCGCA GATATTCAAT CCGCAGCCTG TGAAACCAGA GCCCCTGGCC 
CAACCACCGC GCCCACTCAG CAATGCCAGC TCTTCAGGTG GTCCGCCGCC AGCAGGCGGC 
GCATCATCCT CCGGCGGATC GGGTCTTTCT GGCCGCTCCA AATACGTTTT GGACCCATCT 
GTAGCAGCTC CAACCAGTTC CTACGGAATG CCATACAATC CAGTGCCGGC CCCCGTTCCA 
TCAGCGGTTC CATTTGGCGG CGGAGGAGTG CCTGGGCCCG CACCCCAGCC AGCTAGCGTG 
CCAACGTACA ACAGCAACGC GTTCAACACG AATCCTATTG CGGCTGCCCA ACCTTACAAC 
CCTTCGCCAC TCATGCCAGC TCAGCAGAAT CAGTTCGTGC CCGGTGTGAA TCCCATCGAG 
ACAATGCCAC CGCCGGCTGT GCCACCAGTC ATCCAGAATG TGCAACGCAA TCCCACACCA 
CCACCTGGCT GGAACGATCC TCCTGCACTG AAATCTTCGC GAGCGCCTAA GCCGAAGCCG 
GTGGTTGAGC CAAGTGCTGC TATCTTCCAT CCCCTGTTCC CAGTCGACCC GAATCAGAAC 
CAGAACGGTT ATGTGGACCC CTCTCAATAT CAGCACGCAG CACCACTTGG AGGAGTGCCC 
AATACGTACT ACAACCCGGC TGCCTTCAAC AACAATCCCC AGCAGCAGCA GCAGCATCAG 
CAATCATATC TCCAGCCTCA GCAGCCAGCC CCTGGGATTC AGCAGCCAAA CTGGCAGGGA 
CAACAGCAAA CCATTGGCTA TACCGAGCAG CCAGCGCCCA TCCAGCAACA GCGCCAACCG 
GCGCCGCCCA AGGAGAAGCC ACCACTCCCC GAGGAGTACA TCTACTTGCA GACTGTGCTG 
GAGGAGCTGA AGAACCGCTG CCTGGGCGCA ACCAATGATC CGCGCACAAA ACGAAAGTTT 
GTCGATGTTG TCAAGAGGCT CGAAAATCTC TACGACTGCC TGAGAGATGG AAAGCTCACT 
CCCACCACGG TGCAATATCT GAATCAAATA GTTCAGTACA TTCAAATATC CGACTATGCC 
AATGCTATGG AAATCCACAC GCAAATCGCC TTCGGAACGG ACTTTGCGCA GTGCGCGGGC 
TTTATGCAGG GACTGAAGGT CCTACTGCAA TCCGCATCCG AGCTACAGTT GGTTCTGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286215.1
CDS	145..3927
Translation	MKIKELQKTVNIAWSPAQQQQILLAAGTAAQQFDSNANSTLELYSPNFSDATYDLELRASVSSQYKFQKLIWSPVGPHPSGLIVGGCEAGQINIYSADKLLAGKEEPLLKRQDKHTGAVSGLDFNPFQNNLLASCASESEILIWDLNNPETSMSPGTKTQPLEDVKNVAWNRQVQHILASVFSTRCVIWDLRKSQQIIKLSDSQSRVRWHAIEWHPEAATQVWLASEDDQAPVVQLWDLRYATAPAKTYQIHERGVLGMSWCLQDNDLMVSCGKDNRIYCWNPNTKIPEGEILSEVATTASWYSDVQFCPRNPALIASASLEGAVSIYSLHGGTHHLVQTANKIADSFPGMDQFAQEPIPQQATQVVYHDLAHAPKWMKRPCGVAFGFGGKLVSFNGTSKTVKVQQLTTESALVDRANALERSLVDANYSDYCRQRADETPDQHGRYIWYFIKANFELNPKEEMLNLLGYNKDDIDSKFNKFIKETDGNSHSDVDTLTTRISTLTHSDSSEVECDQSTNYSTDNSQTQDNKFDGNAIDSQNHNDFATPPRPQFKVPSGDHSDSLIAEAILTGNVDAAVELCLEAQRIPEALIIASTAGIETLNRTQTRYLLQQKNELSHVISALVSRDWLDFVNRCTVDSWKEALVAALKHSERKVVDICERLGDRLLSECASSAEFTRNAMLCYICAGSIDKLVTAWYQLKRLEQQNPGYKPNTTELQDLAEMVMLMCKSLEQQGISVDLAGRFAGFLTEYGGLLASQGALTAALQYITTLGGGAAASESDVLQSLRDRINNAVNLDYTQPQAAVAAAPHGYQPTRGRGSFSQPQPSAPMPYGQPPNQYAQPSWPAQVPPLQTSYPGAQQPQAPPQIFNPQPVKPEPLAQPPRPLSNASSSGGPPPAGGASSSGGSGLSGRSKYVLDPSVAAPTSSYGMPYNPVPAPVPSAVPFGGGGVPGPAPQPASVPTYNSNAFNTNPIAAAQPYNPSPLMPAQQNQFVPGVNPIETMPPPAVPPVIQNVQRNPTPPPGWNDPPALKSSRAPKPKPVVEPSAAIFHPLFPVDPNQNQNGYVDPSQYQHAAPLGGVPNTYYNPAAFNNNPQQQQQHQQSYLQPQQPAPGIQQPNWQGQQQTIGYTEQPAPIQQQRQPAPPKEKPPLPEEYIYLQTVLEELKNRCLGATNDPRTKRKFVDVVKRLENLYDCLRDGKLTPTTVQYLNQIVQYIQISDYANAMEIHTQIAFGTDFAQCAGFMQGLKVLLQSASELQLVLR

Target ORF information:

RefSeq Version	NM_001299286.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 31, transcript variant D (Sec31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299286.1

ORF Insert Sequence:

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATAG CGACTCTTCT GAGGTCGAGT GTGATCAGAG TACTAACTAT 
AGCACCGATA ATTCGCAGAC GCAAGATAAC AAGTTTGATG GCAATGCAAT CGACTCACAA 
AATCACAACG ACTTTGCCAC TCCTCCGAGG CCGCAATTCA AGGTTCCCAG CGGAGACCAC 
TCGGATAGCC TGATTGCCGA GGCAATACTC ACTGGCAATG TGGACGCTGC CGTGGAGCTC 
TGTCTGGAAG CACAGCGCAT TCCTGAAGCC CTGATTATCG CCTCAACGGC TGGCATTGAG 
ACTCTGAACC GCACGCAAAC GCGCTACCTG CTGCAGCAGA AGAACGAGCT ATCGCACGTT 
ATCTCCGCTT TGGTATCGCG CGACTGGTTG GACTTCGTCA ACAGATGCAC TGTGGACTCG 
TGGAAGGAGG CCCTGGTGGC CGCGCTCAAG CACAGCGAGC GCAAGGTGGT GGACATCTGC 
GAGCGACTAG GCGATCGCCT CTTAAGTGAG TGCGCCTCAA GCGCCGAGTT TACGCGCAAC 
GCCATGCTCT GCTACATTTG CGCCGGCAGC ATCGACAAAC TGGTCACCGC GTGGTACCAG 
TTAAAGCGGC TGGAGCAGCA GAACCCCGGC TACAAGCCCA ACACCACAGA GCTGCAGGAC 
TTGGCTGAGA TGGTGATGCT GATGTGCAAG TCGTTGGAGC AGCAAGGCAT ATCCGTGGAC 
TTGGCTGGTC GCTTTGCCGG CTTTCTGACT GAGTACGGCG GTCTTCTTGC GTCCCAGGGT 
GCTCTGACTG CAGCCCTGCA GTACATTACC ACCTTGGGCG GAGGAGCTGC CGCCAGCGAA 
AGCGATGTTC TGCAGAGCCT ACGAGACCGG ATAAATAACG CTGTCAACCT GGACTACACC 
CAGCCACAGG CAGCAGTCGC TGCTGCGCCA CACGGCTACC AACCGACTCG TGGTCGTGGA 
TCGTTCTCGC AGCCACAGCC TTCGGCTCCC ATGCCCTACG GCCAGCCGCC CAATCAGTAT 
GCACAGCCCA GCTGGCCAGC GCAAGTGCCT CCACTACAAA CAAGCTACCC AGGAGCCCAG 
CAGCCTCAGG CTCCGCCGCA GATATTCAAT CCGCAGCCTG TGAAACCAGA GCCCCTGGCC 
CAACCACCGC GCCCACTCAG CAATGCCAGC TCTTCAGGTG GTCCGCCGCC AGCAGGCGGC 
GCATCATCCT CCGGCGGATC GGGTCTTTCT GGCCGCTCCA AATACGTTTT GGACCCATCT 
GTAGCAGCTC CAACCAGTTC CTACGGAATG CCATACAATC CAGTGCCGGC CCCCGTTCCA 
TCAGCGGTTC CATTTGGCGG CGGAGGAGTG CCTGGGCCCG CACCCCAGCC AGCTAGCGTG 
CCAACGTACA ACAGCAACGC GTTCAACACG AATCCTATTG CGGCTGCCCA ACCTTACAAC 
CCTTCGCCAC TCATGCCAGC TCAGCAGAAT CAGTTCGTGC CCGGTGTGAA TCCCATCGAG 
ACAATGCCAC CGCCGGCTGT GCCACCAGTC ATCCAGAATG TGCAACGCAA TCCCACACCA 
CCACCTGGCT GGAACGATCC TCCTGCACTG AAATCTTCGC GAGCGCCTAA GCCGAAGCCG 
GTGGTTGAGC CAAGTGCTGC TATCTTCCAT CCCCTGTTCC CAGTCGACCC GAATCAGAAC 
CAGAACGGTT ATGTGGACCC CTCTCAATAT CAGCACGCAG CACCACTTGG AGGAGTGCCC 
AATACGTACT ACAACCCGGC TGCCTTCAAC AACAATCCCC AGCAGCAGCA GCAGCATCAG 
CAATCATATC TCCAGCCTCA GCAGCCAGCC CCTGGGATTC AGCAGCCAAA CTGGCAGGGA 
CAACAGCAAA CCATTGGCTA TACCGAGCAG CCAGCGCCCA TCCAGCAACA GCGCCAACCG 
GCGCCGCCCA AGGAGAAGCC ACCACTCCCC GAGGAGTACA TCTACTTGCA GACTGTGCTG 
GAGGAGCTGA AGAACCGCTG CCTGGGCGCA ACCAATGATC CGCGCACAAA ACGAAAGTTT 
GTCGATGTTG TCAAGAGGCT CGAAAATCTC TACGACTGCC TGAGAGATGG AAAGCTCACT 
CCCACCACGG TGCAATATCT GAATCAAATA GTTCAGTACA TTCAAATATC CGACTATGCC 
AATGCTATGG AAATCCACAC GCAAATCGCC TTCGGAACGG ACTTTGCGCA GTGCGCGGGC 
TTTATGCAGG GACTGAAGGT CCTACTGCAA TCCGCATCCG AGCTACAGTT GGTTCTGCGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec31 ( NM_001299285.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286214.1 Drosophila melanogaster secretory 31, transcript variant C (Sec31), mRNA.

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CloneID	OFa21722
Clone ID Related Accession (Same CDS sequence)	NM_001299285.1
Accession Version	NM_001299285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 31, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATAG CGACTCTTCT GAGGTCGAGT GTGATCAGAG TACTAACTAT 
AGCACCGATA ATTCGACGCA AGATAACAAG TTTGATGGCA ATGCAATCGA CTCACAAAAT 
CACAACGACT TTGCCACTCC TCCGAGGCCG CAATTCAAGG TTCCCAGCGG AGACCACTCG 
GATAGCCTGA TTGCCGAGGC AATACTCACT GGCAATGTGG ACGCTGCCGT GGAGCTCTGT 
CTGGAAGCAC AGCGCATTCC TGAAGCCCTG ATTATCGCCT CAACGGCTGG CATTGAGACT 
CTGAACCGCA CGCAAACGCG CTACCTGCTG CAGCAGAAGA ACGAGCTATC GCACGTTATC 
TCCGCTTTGG TATCGCGCGA CTGGTTGGAC TTCGTCAACA GATGCACTGT GGACTCGTGG 
AAGGAGGCCC TGGTGGCCGC GCTCAAGCAC AGCGAGCGCA AGGTGGTGGA CATCTGCGAG 
CGACTAGGCG ATCGCCTCTT AAGTGAGTGC GCCTCAAGCG CCGAGTTTAC GCGCAACGCC 
ATGCTCTGCT ACATTTGCGC CGGCAGCATC GACAAACTGG TCACCGCGTG GTACCAGTTA 
AAGCGGCTGG AGCAGCAGAA CCCCGGCTAC AAGCCCAACA CCACAGAGCT GCAGGACTTG 
GCTGAGATGG TGATGCTGAT GTGCAAGTCG TTGGAGCAGC AAGGCATATC CGTGGACTTG 
GCTGGTCGCT TTGCCGGCTT TCTGACTGAG TACGGCGGTC TTCTTGCGTC CCAGGGTGCT 
CTGACTGCAG CCCTGCAGTA CATTACCACC TTGGGCGGAG GAGCTGCCGC CAGCGAAAGC 
GATGTTCTGC AGAGCCTACG AGACCGGATA AATAACGCTG TCAACCTGGA CTACACCCAG 
CCACAGGCAG CAGTCGCTGC TGCGCCACAC GGCTACCAAC CGACTCGTGG TCGTGGATCG 
TTCTCGCAGC CACAGCCTTC GGCTCCCATG CCCTACGGCC AGCCGCCCAA TCAGTATGCA 
CAGCCCAGCT GGCCAGCGCA AGTGCCTCCA CTACAAACAA GCTACCCAGG AGCCCAGCAG 
CCTCAGGCTC CGCCGCAGAT ATTCAATCCG CAGCCTGTGA AACCAGAGCC CCTGGCCCAA 
CCACCGCGCC CACTCAGCAA TGCCAGCTCT TCAGGTGGTC CGCCGCCAGC AGGCGGCGCA 
TCATCCTCCG GCGGATCGGG TCTTTCTGGC CGCTCCAAAT ACGTTTTGGA CCCATCTGTA 
GCAGCTCCAA CCAGTTCCTA CGGAATGCCA TACAATCCAG TGCCGGCCCC CGTTCCATCA 
GCGGTTCCAT TTGGCGGCGG AGGAGTGCCT GGGCCCGCAC CCCAGCCAGC TAGCGTGCCA 
ACGTACAACA GCAACGCGTT CAACACGAAT CCTATTGCGG CTGCCCAACC TTACAACCCT 
TCGCCACTCA TGCCAGCTCA GCAGAATCAG TTCGTGCCCG GTGTGAATCC CATCGAGACA 
ATGCCACCGC CGGCTGTGCC ACCAGTCATC CAGAATGTGC AACGCAATCC CACACCACCA 
CCTGGCTGGA ACGATCCTCC TGCACTGAAA TCTTCGCGAG CGCCTAAGCC GAAGCCGGTG 
GTTGAGCCAA GTGCTGCTAT CTTCCATCCC CTGTTCCCAG TCGACCCGAA TCAGAACCAG 
AACGGTTATG TGGACCCCTC TCAATATCAG CACGCAGCAC CACTTGGAGG AGTGCCCAAT 
ACGTACTACA ACCCGGCTGC CTTCAACAAC AATCCCCAGC AGCAGCAGCA GCATCAGCAA 
TCATATCTCC AGCCTCAGCA GCCAGCCCCT GGGATTCAGC AGCCAAACTG GCAGGGACAA 
CAGCAAACCA TTGGCTATAC CGAGCAGCCA GCGCCCATCC AGCAACAGCG CCAACCGGCG 
CCGCCCAAGG AGAAGCCACC ACTCCCCGAG GAGTACATCT ACTTGCAGAC TGTGCTGGAG 
GAGCTGAAGA ACCGCTGCCT GGGCGCAACC AATGATCCGC GCACAAAACG AAAGTTTGTC 
GATGTTGTCA AGAGGCTCGA AAATCTCTAC GACTGCCTGA GAGATGGAAA GCTCACTCCC 
ACCACGGTGC AATATCTGAA TCAAATAGTT CAGTACATTC AAATATCCGA CTATGCCAAT 
GCTATGGAAA TCCACACGCA AATCGCCTTC GGAACGGACT TTGCGCAGTG CGCGGGCTTT 
ATGCAGGGAC TGAAGGTCCT ACTGCAATCC GCATCCGAGC TACAGTTGGT TCTGCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286214.1
CDS	145..3924
Translation	MKIKELQKTVNIAWSPAQQQQILLAAGTAAQQFDSNANSTLELYSPNFSDATYDLELRASVSSQYKFQKLIWSPVGPHPSGLIVGGCEAGQINIYSADKLLAGKEEPLLKRQDKHTGAVSGLDFNPFQNNLLASCASESEILIWDLNNPETSMSPGTKTQPLEDVKNVAWNRQVQHILASVFSTRCVIWDLRKSQQIIKLSDSQSRVRWHAIEWHPEAATQVWLASEDDQAPVVQLWDLRYATAPAKTYQIHERGVLGMSWCLQDNDLMVSCGKDNRIYCWNPNTKIPEGEILSEVATTASWYSDVQFCPRNPALIASASLEGAVSIYSLHGGTHHLVQTANKIADSFPGMDQFAQEPIPQQATQVVYHDLAHAPKWMKRPCGVAFGFGGKLVSFNGTSKTVKVQQLTTESALVDRANALERSLVDANYSDYCRQRADETPDQHGRYIWYFIKANFELNPKEEMLNLLGYNKDDIDSKFNKFIKETDGNSHSDVDTLTTRISTLTHSDSSEVECDQSTNYSTDNSTQDNKFDGNAIDSQNHNDFATPPRPQFKVPSGDHSDSLIAEAILTGNVDAAVELCLEAQRIPEALIIASTAGIETLNRTQTRYLLQQKNELSHVISALVSRDWLDFVNRCTVDSWKEALVAALKHSERKVVDICERLGDRLLSECASSAEFTRNAMLCYICAGSIDKLVTAWYQLKRLEQQNPGYKPNTTELQDLAEMVMLMCKSLEQQGISVDLAGRFAGFLTEYGGLLASQGALTAALQYITTLGGGAAASESDVLQSLRDRINNAVNLDYTQPQAAVAAAPHGYQPTRGRGSFSQPQPSAPMPYGQPPNQYAQPSWPAQVPPLQTSYPGAQQPQAPPQIFNPQPVKPEPLAQPPRPLSNASSSGGPPPAGGASSSGGSGLSGRSKYVLDPSVAAPTSSYGMPYNPVPAPVPSAVPFGGGGVPGPAPQPASVPTYNSNAFNTNPIAAAQPYNPSPLMPAQQNQFVPGVNPIETMPPPAVPPVIQNVQRNPTPPPGWNDPPALKSSRAPKPKPVVEPSAAIFHPLFPVDPNQNQNGYVDPSQYQHAAPLGGVPNTYYNPAAFNNNPQQQQQHQQSYLQPQQPAPGIQQPNWQGQQQTIGYTEQPAPIQQQRQPAPPKEKPPLPEEYIYLQTVLEELKNRCLGATNDPRTKRKFVDVVKRLENLYDCLRDGKLTPTTVQYLNQIVQYIQISDYANAMEIHTQIAFGTDFAQCAGFMQGLKVLLQSASELQLVLR

Target ORF information:

RefSeq Version	NM_001299285.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 31, transcript variant C (Sec31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299285.1

ORF Insert Sequence:

ATGAAGATCA AGGAACTGCA AAAGACGGTG AACATCGCCT GGTCGCCGGC GCAGCAGCAG 
CAGATTCTCT TGGCCGCCGG CACCGCCGCC CAACAATTCG ATTCCAATGC CAATTCCACT 
CTGGAGTTGT ACTCACCGAA CTTCAGCGAT GCCACATACG ATCTGGAGCT GAGGGCCAGC 
GTTTCCAGCC AGTACAAGTT CCAAAAACTC ATCTGGTCCC CAGTGGGACC ACACCCCAGT 
GGCCTGATTG TGGGCGGCTG CGAGGCGGGG CAGATCAACA TATACTCAGC CGACAAGCTG 
CTTGCAGGCA AGGAGGAGCC GCTTCTGAAG CGCCAGGACA AGCACACAGG CGCCGTTAGC 
GGGTTGGACT TCAATCCCTT CCAGAACAAC CTGCTGGCCT CATGCGCCTC CGAATCGGAA 
ATCCTAATCT GGGACCTCAA CAACCCGGAA ACAAGCATGA GTCCCGGCAC CAAGACACAA 
CCACTGGAGG ACGTAAAGAA CGTAGCATGG AACAGGCAGG TGCAGCACAT TCTAGCCTCC 
GTGTTCAGCA CACGCTGCGT GATTTGGGAT CTGCGTAAGA GTCAGCAGAT CATCAAGCTC 
TCCGACTCGC AGTCGCGAGT GAGGTGGCAC GCTATTGAGT GGCATCCGGA GGCGGCGACG 
CAGGTGTGGC TGGCTTCCGA GGACGACCAG GCGCCAGTGG TGCAGCTGTG GGATCTGCGA 
TATGCCACGG CTCCAGCCAA AACATACCAG ATTCATGAGC GCGGAGTGCT GGGAATGTCG 
TGGTGCCTGC AGGACAACGA TCTAATGGTT TCGTGTGGCA AAGACAACCG CATCTATTGC 
TGGAACCCCA ATACCAAGAT TCCAGAGGGA GAAATCCTAT CTGAGGTTGC CACCACTGCC 
TCGTGGTACT CCGATGTGCA GTTTTGCCCA CGTAATCCAG CTCTGATAGC TAGCGCCAGC 
TTGGAGGGAG CAGTTTCCAT TTACTCTCTA CATGGAGGCA CTCACCACCT TGTGCAAACC 
GCCAACAAGA TTGCCGACTC GTTTCCGGGC ATGGATCAGT TTGCCCAGGA GCCAATTCCG 
CAGCAGGCCA CGCAAGTGGT TTACCATGAC CTGGCGCACG CTCCCAAATG GATGAAGCGG 
CCCTGTGGCG TTGCCTTTGG ATTCGGTGGC AAGTTGGTCA GTTTCAATGG AACCTCGAAA 
ACGGTCAAGG TCCAACAGCT AACCACCGAA TCCGCCCTTG TGGACCGGGC TAATGCCCTG 
GAACGCTCTC TGGTCGATGC CAATTATTCG GATTACTGTC GGCAGCGAGC AGACGAGACA 
CCGGATCAGC ATGGCCGCTA CATCTGGTAC TTTATTAAGG CGAACTTCGA GCTTAATCCC 
AAGGAGGAAA TGCTGAACCT TTTGGGCTAC AACAAAGACG ACATCGATAG CAAGTTCAAC 
AAGTTTATCA AGGAAACGGA TGGCAACTCG CATTCGGATG TGGATACTCT GACGACCCGC 
ATCTCGACAC TGACCCATAG CGACTCTTCT GAGGTCGAGT GTGATCAGAG TACTAACTAT 
AGCACCGATA ATTCGACGCA AGATAACAAG TTTGATGGCA ATGCAATCGA CTCACAAAAT 
CACAACGACT TTGCCACTCC TCCGAGGCCG CAATTCAAGG TTCCCAGCGG AGACCACTCG 
GATAGCCTGA TTGCCGAGGC AATACTCACT GGCAATGTGG ACGCTGCCGT GGAGCTCTGT 
CTGGAAGCAC AGCGCATTCC TGAAGCCCTG ATTATCGCCT CAACGGCTGG CATTGAGACT 
CTGAACCGCA CGCAAACGCG CTACCTGCTG CAGCAGAAGA ACGAGCTATC GCACGTTATC 
TCCGCTTTGG TATCGCGCGA CTGGTTGGAC TTCGTCAACA GATGCACTGT GGACTCGTGG 
AAGGAGGCCC TGGTGGCCGC GCTCAAGCAC AGCGAGCGCA AGGTGGTGGA CATCTGCGAG 
CGACTAGGCG ATCGCCTCTT AAGTGAGTGC GCCTCAAGCG CCGAGTTTAC GCGCAACGCC 
ATGCTCTGCT ACATTTGCGC CGGCAGCATC GACAAACTGG TCACCGCGTG GTACCAGTTA 
AAGCGGCTGG AGCAGCAGAA CCCCGGCTAC AAGCCCAACA CCACAGAGCT GCAGGACTTG 
GCTGAGATGG TGATGCTGAT GTGCAAGTCG TTGGAGCAGC AAGGCATATC CGTGGACTTG 
GCTGGTCGCT TTGCCGGCTT TCTGACTGAG TACGGCGGTC TTCTTGCGTC CCAGGGTGCT 
CTGACTGCAG CCCTGCAGTA CATTACCACC TTGGGCGGAG GAGCTGCCGC CAGCGAAAGC 
GATGTTCTGC AGAGCCTACG AGACCGGATA AATAACGCTG TCAACCTGGA CTACACCCAG 
CCACAGGCAG CAGTCGCTGC TGCGCCACAC GGCTACCAAC CGACTCGTGG TCGTGGATCG 
TTCTCGCAGC CACAGCCTTC GGCTCCCATG CCCTACGGCC AGCCGCCCAA TCAGTATGCA 
CAGCCCAGCT GGCCAGCGCA AGTGCCTCCA CTACAAACAA GCTACCCAGG AGCCCAGCAG 
CCTCAGGCTC CGCCGCAGAT ATTCAATCCG CAGCCTGTGA AACCAGAGCC CCTGGCCCAA 
CCACCGCGCC CACTCAGCAA TGCCAGCTCT TCAGGTGGTC CGCCGCCAGC AGGCGGCGCA 
TCATCCTCCG GCGGATCGGG TCTTTCTGGC CGCTCCAAAT ACGTTTTGGA CCCATCTGTA 
GCAGCTCCAA CCAGTTCCTA CGGAATGCCA TACAATCCAG TGCCGGCCCC CGTTCCATCA 
GCGGTTCCAT TTGGCGGCGG AGGAGTGCCT GGGCCCGCAC CCCAGCCAGC TAGCGTGCCA 
ACGTACAACA GCAACGCGTT CAACACGAAT CCTATTGCGG CTGCCCAACC TTACAACCCT 
TCGCCACTCA TGCCAGCTCA GCAGAATCAG TTCGTGCCCG GTGTGAATCC CATCGAGACA 
ATGCCACCGC CGGCTGTGCC ACCAGTCATC CAGAATGTGC AACGCAATCC CACACCACCA 
CCTGGCTGGA ACGATCCTCC TGCACTGAAA TCTTCGCGAG CGCCTAAGCC GAAGCCGGTG 
GTTGAGCCAA GTGCTGCTAT CTTCCATCCC CTGTTCCCAG TCGACCCGAA TCAGAACCAG 
AACGGTTATG TGGACCCCTC TCAATATCAG CACGCAGCAC CACTTGGAGG AGTGCCCAAT 
ACGTACTACA ACCCGGCTGC CTTCAACAAC AATCCCCAGC AGCAGCAGCA GCATCAGCAA 
TCATATCTCC AGCCTCAGCA GCCAGCCCCT GGGATTCAGC AGCCAAACTG GCAGGGACAA 
CAGCAAACCA TTGGCTATAC CGAGCAGCCA GCGCCCATCC AGCAACAGCG CCAACCGGCG 
CCGCCCAAGG AGAAGCCACC ACTCCCCGAG GAGTACATCT ACTTGCAGAC TGTGCTGGAG 
GAGCTGAAGA ACCGCTGCCT GGGCGCAACC AATGATCCGC GCACAAAACG AAAGTTTGTC 
GATGTTGTCA AGAGGCTCGA AAATCTCTAC GACTGCCTGA GAGATGGAAA GCTCACTCCC 
ACCACGGTGC AATATCTGAA TCAAATAGTT CAGTACATTC AAATATCCGA CTATGCCAAT 
GCTATGGAAA TCCACACGCA AATCGCCTTC GGAACGGACT TTGCGCAGTG CGCGGGCTTT 
ATGCAGGGAC TGAAGGTCCT ACTGCAATCC GCATCCGAGC TACAGTTGGT TCTGCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sec31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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