Vars2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vars2
Entrez Gene ID	68915
Full Name	valyl-tRNA synthetase 2, mitochondrial
Synonyms	1190004I24Rik,Vars2l,mKIAA1885
General protein information	Preferred Names valyl-tRNA synthetase 2, mitochondrial Names valine--tRNA ligase, mitochondrial valRS valyl-tRNA synthetase 2, mitochondrial (putative) valyl-tRNA synthetase, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030250036.1	XP_030105896.1	valine--tRNA ligase, mitochondrial isoform X2
XM_011246589.2	XP_011244891.1	valine--tRNA ligase, mitochondrial isoform X1
XM_011246589.3	XP_011244891.1	valine--tRNA ligase, mitochondrial isoform X1
NM_175137.4	NP_780346.3	valine--tRNA ligase, mitochondrial precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VARS2	NP_001243473.1
Mus musculus (house mouse)	Vars2	NP_780346.3
Rattus norvegicus (Norway rat)	Vars2	NP_998728.1
Danio rerio (zebrafish)	vars2	NP_001124133.1
Xenopus tropicalis (tropical clawed frog)	LOC100493727	XP_004917090.1
Homo sapiens (human)	VARS2	NP_001161206.1
Canis lupus familiaris (dog)	VARS2	XP_005627180.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of early nephron patterning reveals a role for distal RV proliferation in fusion to the ureteric tip via a cap mesenchyme-derived connecting segment.
Georgas K, Rumballe B, Valerius MT, Chiu HS, Thiagarajan RD, Lesieur E, Aronow BJ, Brunskill EW, Combes AN, Tang D, Taylor D, Grimmond SM, Potter SS, McMahon AP, Little MH
Developmental biology332(2)273-86(2009 Aug)

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53980	XM_030250036.1 Latest version!	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu70354	XM_011246589.3 Latest version!	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu21126	NM_175137.4 Latest version!	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$622.30 $889.00
View Old Accession Versions >>
OMu70354	XM_011246589.2	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vars2 ( XM_011246589.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244891.1 Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70354
Clone ID Related Accession (Same CDS sequence)	XM_011246589.2 , XM_011246589.3
Accession Version	XM_011246589.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG 
GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC 
AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC 
CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG 
CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT 
GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG 
CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG 
GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC 
GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA 
TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC 
CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC 
CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT 
GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT 
CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT 
GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT 
CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC 
CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC 
ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG 
GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC 
TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA 
GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA 
TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC 
ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC 
ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA 
GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG 
GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244891.1
CDS	788..3550
Translation	MCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWIPGSDHAGIATQAMVEKQLWKEQRVRRHELSREDFLRAVWQWKHEKGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYNSGLLYRNRQLVNWSCTLRSAISDIEVESRPLPGRTVLQLPGCPTPVSFGLLASVAFPVDGEPDTEIVVGTTRPETLPGDVAVAVHPDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRGLQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAEARAVAAKRTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPRETPDLAHFYPLTLLETGSDLLMFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDPGELAVAAAAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFVLRALGDNFVPQPAEEVTPSSPMDAWILSRLAFAASECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPVLSSVPRPPGPPQVLFSCADVGLRLLAPLMPFLAEELWQRLPPRPGGPLAPSICVAPYPSTRSLEFWRQPELERCFSRVQEVVQALRALRATYQLTKARPQVLLQCSDPGEQGLVQPFLEPLGILSHCGAVGFLSPGAAAPSGWALTPLGDTMKIYMELQGLVDPQSQLPRLTARRQKLQKQLDDLLNRTMSEGLAERQQRISSLHLELSKLDQAASYLQQLMDEAPSAREL

Target ORF information:

RefSeq Version	XM_011246589.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246589.2

ORF Insert Sequence:

ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG 
GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC 
AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC 
CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG 
CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT 
GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG 
CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG 
GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC 
GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA 
TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC 
CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC 
CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT 
GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT 
CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT 
GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT 
CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC 
CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC 
ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG 
GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC 
TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA 
GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA 
TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC 
ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC 
ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA 
GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG 
GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vars2 ( XM_030250036.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105896.1 Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu53980
Clone ID Related Accession (Same CDS sequence)	XM_030250036.1
Accession Version	XM_030250036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT 
GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG 
GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG 
CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGCTGT 
GGTGGGGCCA TCAGATTCCA GCCTACCGGG TCATCGGGGA GAATGCAGAG GATGACAGGA 
AGGAATGTTG GGTGGTCGGA CGGTCAGAGG CTGAGGCCAG AGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105896.1
CDS	367..2034
Translation	MPHLPLASFRPPLWGLRPSWGLSRPQALCTQPEPHGSPVSRRNREAKQKRLREKQAALEAGLAEKSKIPAVPTKAWSHKEVVLYEIPTGPGEKKDVSGPLPPAYSPQYVEAAWYQWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWIPGSDHAGIATQAMVEKQLWKEQRVRRHELSREDFLRAVWQWKHEKGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYNSGLLYRNRQLVNWSCTLRSAISDIEVESRPLPGRTVLQLPGCPTPVSFGLLASVAFPVDGEPDTEIVVGTTRPETLPGDVAVAVHPDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRGLQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGCGGAIRFQPTGSSGRMQRMTGRNVGWSDGQRLRPER

Target ORF information:

RefSeq Version	XM_030250036.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250036.1

ORF Insert Sequence:

ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT 
GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG 
GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG 
CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGCTGT 
GGTGGGGCCA TCAGATTCCA GCCTACCGGG TCATCGGGGA GAATGCAGAG GATGACAGGA 
AGGAATGTTG GGTGGTCGGA CGGTCAGAGG CTGAGGCCAG AGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vars2 ( XM_011246589.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244891.1 Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.

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CloneID	OMu70354
Clone ID Related Accession (Same CDS sequence)	XM_011246589.2 , XM_011246589.3
Accession Version	XM_011246589.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246589.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG 
GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC 
AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC 
CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG 
CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT 
GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG 
CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG 
GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC 
GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA 
TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC 
CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC 
CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT 
GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT 
CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT 
GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT 
CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC 
CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC 
ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG 
GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC 
TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA 
GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA 
TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC 
ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC 
ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA 
GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG 
GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244891.1
CDS	794..3556
Translation	MCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWIPGSDHAGIATQAMVEKQLWKEQRVRRHELSREDFLRAVWQWKHEKGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYNSGLLYRNRQLVNWSCTLRSAISDIEVESRPLPGRTVLQLPGCPTPVSFGLLASVAFPVDGEPDTEIVVGTTRPETLPGDVAVAVHPDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRGLQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAEARAVAAKRTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPRETPDLAHFYPLTLLETGSDLLMFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDPGELAVAAAAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFVLRALGDNFVPQPAEEVTPSSPMDAWILSRLAFAASECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPVLSSVPRPPGPPQVLFSCADVGLRLLAPLMPFLAEELWQRLPPRPGGPLAPSICVAPYPSTRSLEFWRQPELERCFSRVQEVVQALRALRATYQLTKARPQVLLQCSDPGEQGLVQPFLEPLGILSHCGAVGFLSPGAAAPSGWALTPLGDTMKIYMELQGLVDPQSQLPRLTARRQKLQKQLDDLLNRTMSEGLAERQQRISSLHLELSKLDQAASYLQQLMDEAPSAREL

Target ORF information:

RefSeq Version	XM_011246589.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246589.3

ORF Insert Sequence:

ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG 
GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC 
AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC 
CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG 
CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT 
GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG 
CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG 
GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC 
GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA 
TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC 
CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC 
CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT 
GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT 
CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT 
GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT 
CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC 
CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC 
ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG 
GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC 
TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA 
GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA 
TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC 
ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC 
ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA 
GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG 
GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vars2 ( NM_175137.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780346.3 Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), mRNA.

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CloneID	OMu21126
Clone ID Related Accession (Same CDS sequence)	NM_175137.4
Accession Version	NM_175137.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK129008.1, CA327239.1, CD355876.1, CX563092.1, AK038117.1, AK004481.1 and BC057036.1. On Nov 15, 2007 this sequence version replaced NM_175137.3. ##Evidence-Data-START## Transcript exon combination :: AK129008.1, AK155386.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT 
GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG 
GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG 
CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG 
GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC 
AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC 
CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG 
CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT 
GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG 
CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG 
GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC 
GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA 
TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC 
CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC 
CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT 
GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT 
CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT 
GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT 
CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC 
CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC 
ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG 
GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC 
TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA 
GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA 
TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC 
ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC 
ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA 
GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG 
GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780346.3
CDS	320..3502
Translation	MPHLPLASFRPPLWGLRPSWGLSRPQALCTQPEPHGSPVSRRNREAKQKRLREKQAALEAGLAEKSKIPAVPTKAWSHKEVVLYEIPTGPGEKKDVSGPLPPAYSPQYVEAAWYQWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWIPGSDHAGIATQAMVEKQLWKEQRVRRHELSREDFLRAVWQWKHEKGGEIYEQLCALGASLDWDRECFTMDAGSSAAVTEAFVRLYNSGLLYRNRQLVNWSCTLRSAISDIEVESRPLPGRTVLQLPGCPTPVSFGLLASVAFPVDGEPDTEIVVGTTRPETLPGDVAVAVHPDDPRYTHLHGRQLRHPLTGQLLPLITDTTVQPHVGTGAVKVTPAHSPIDAEIGTRHGLTPLSVIAEDGTMTSLCGDWLQGLHRFVAREKIMCTLREQGLFRGLQEHPMVLPICSRSGDVVEYLLKSQWFVRCQEMGDLAAKAVESGALELWPSFHQKSWQHWFAHIGDWCVSRQLWWGHQIPAYRVIGENAEDDRKECWVVGRSEAEARAVAAKRTGRPEAELTLERDPDVLDTWFSSALFPFSALGWPRETPDLAHFYPLTLLETGSDLLMFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGQELQVLQAKLRDGNLDPGELAVAAAAQKKDFPYGIPECGTDALRFALCSHGILGGDLHLSVSEVLNYRHFCNKLWNALRFVLRALGDNFVPQPAEEVTPSSPMDAWILSRLAFAASECERGFLSRELSLVTHTLYHFWLHNLCDVYLEAVKPVLSSVPRPPGPPQVLFSCADVGLRLLAPLMPFLAEELWQRLPPRPGGPLAPSICVAPYPSTRSLEFWRQPELERCFSRVQEVVQALRALRATYQLTKARPQVLLQCSDPGEQGLVQPFLEPLGILSHCGAVGFLSPGAAAPSGWALTPLGDTMKIYMELQGLVDPQSQLPRLTARRQKLQKQLDDLLNRTMSEGLAERQQRISSLHLELSKLDQAASYLQQLMDEAPSAREL

Target ORF information:

RefSeq Version	NM_175137.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175137.4

ORF Insert Sequence:

ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT 
GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG 
GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG 
CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC 
CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG 
GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG 
TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG 
GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT 
TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA 
TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC 
ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT 
GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG 
ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA 
CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC 
ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA 
GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG 
ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA 
AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG 
GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG 
GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC 
AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC 
CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG 
CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT 
GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG 
CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG 
GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC 
GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA 
TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC 
CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC 
CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT 
GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT 
CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT 
GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT 
CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC 
CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC 
ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG 
GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC 
TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA 
GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA 
TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC 
ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC 
ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA 
GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG 
GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of early nephron patterning reveals a role for distal RV proliferation in fusion to the ureteric tip via a cap mesenchyme-derived connecting segment.
Developmental biology332(2)273-86(2009 Aug)
Georgas K,Rumballe B,Valerius MT,Chiu HS,Thiagarajan RD,Lesieur E,Aronow BJ,Brunskill EW,Combes AN,Tang D,Taylor D,Grimmond SM,Potter SS,McMahon AP,Little MH

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)
Okazaki N,F-Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Seino S,Nishimura M,Kaisho T,Hoshino K,Kitamura H,Nagase T,Ohara O,Koga H

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Vars2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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