Zwilch cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zwilch
Entrez Gene ID	68014
Full Name	zwilch kinetochore protein
Synonyms	2310031L18Rik
General protein information	Preferred Names zwilch kinetochore protein Names protein zwilch homolog Zwilch, kinetochore associated, homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_026507.4	NP_080783.3	protein zwilch homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZWILCH	NP_060445.3
Bos taurus (cattle)	ZWILCH	NP_001095649.1
Danio rerio (zebrafish)	zwilch	NP_001186664.1
Mus musculus (house mouse)	Zwilch	NP_080783.3
Gallus gallus (chicken)	ZWILCH	XP_004943968.1
Xenopus tropicalis (tropical clawed frog)	zwilch	NP_001016216.1
	ZWILCH	XP_001110821.2
Pan troglodytes (chimpanzee)	ZWILCH	XP_510495.1
Canis lupus familiaris (dog)	ZWILCH	XP_535523.3
Rattus norvegicus (Norway rat)	Zwilch	XP_006243310.1

Gene Ontology
Bibliography

Related articles in PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

A global genomic transcriptional code associated with CNS-expressed genes.
Bailey PJ, Klos JM, Andersson E, Karl?n M, K?llstr?m M, Ponjavic J, Muhr J, Lenhard B, Sandelin A, Ericson J
Experimental cell research312(16)3108-19(2006 Oct)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Zwilch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zwilch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02460	NM_026507.4 Latest version!	Mus musculus zwilch kinetochore protein (Zwilch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zwilch ( NM_026507.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080783.3 Mus musculus zwilch kinetochore protein (Zwilch), mRNA.

Price & Availability↑

CloneID	OMu02460
Clone ID Related Accession (Same CDS sequence)	NM_026507.4
Accession Version	NM_026507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	protein zwilch homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB839968.1, AK019825.1, BM211747.2 and AC114674.10. On Sep 3, 2009 this sequence version replaced NM_026507.3. CCDS Note: The coding region has been updated to extend the N-terminus. The use of an alternative upstream start codon, which appears to be rodent-specific, would result in a protein that is 7 aa longer. This uAUG also has a strong Kozak signal and has a good chance of being used in these species. ##Evidence-Data-START## Transcript exon combination :: BC027435.1, AK050917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGGAG GCCACGGCGG TATGTGGTCG CGAATGAACC GCGCGGCGGA GGAGTTTTAT 
GCTCGTCTCC GGCAGGAATT TAATGAAGAG AAGAAAGGAG CCTCTAAAGA CCCATTCATC 
TATGAGGCTG ATGTACAAGT GCAGTTGATC AGCAAAGGCC AACCAAGTCT TCTGAAAACT 
ATTCTAAATG AAAATGACTC AGTGTTTCTC GTGGAAAAAG TGGTTTTGGA GAAGGAAGAA 
ACAAGTCAAG TTGAAGAACT GCAGTCTGAA GAAACTGCTA TTTCTGATCT CTCTGCTGGT 
GAAAATATTA GGCCACTGGC CTTACCTGTT GGGAGAGCAA GGCAGTTAAT TGGGCTCTAT 
ACCATGGCTC ACAATCCTAA TATGACTCAT CTGAAGATTA AGCAGCCAGT GACAGCCCTT 
CCTCCTCTTT GGGTGAGATG TGACGGTTCA GATCCTGAAG GTACCTGTTG GCTAGGAGCT 
GAGCTTATCA CAACAAATGA CATCATTGCA GGCGTTATCC TGTATGTGCT CACTTGTAAA 
GCTGATAAAA ATTACTCTGA AGATCTGGAA AATTTAAAAA CTTCACACAA GAAAAGACAT 
CACGTGTCTG CTGTGACAGC CAGAGGCTTT GCCCAGTATG AGCTCTTTAA GTCTGACGAC 
TTGGATGACA CTGTTGCCCC ATCACAAACT ACAGTCACGC TGGACCTTTC CTGGAGCCCT 
GTGGATGAGA TGCTCCAAAC CCCTCCCCTG TCTTCAACTG CAGCTCTGAA TATCAGAGTA 
CAATCAGGAG AGTCCAGAGG TTGTCTGAGT CATCTTCACA GAGAACTGAA ATTTCTCCTT 
GTTTTAGCTG ATGGCATCAG GACTGGTGTA ACTGAATGGC TTGAGCCTCT GGAAACAAAA 
TCTGCTCTTG AATTTGTTCA GGAATTTCTG AATGACTTAA ATAAACTGGA TGAATTTGAT 
GATTCTACAA AAAAAGATAA ACAAAAAGAG GCAGTGAACC ATGACGCAGC TGCAGTTGTC 
AGGTCCATGC TTCTCACCGT GCGGGGGGAT CTCGATTTTG CTGAACAGCT TTGGTGTAGA 
ATGAGCAGCA GTGTGGTTTC CTATCAAGAC TTGGTGAAGT GTTTCACACT CATCCTGCAG 
AGTCTTCAGC GTGGTGATAT ACAGCCATGG TTGCACAGTG GAAGTAACAG TTTACTAAGT 
AAACTCATTC ATCAGTCTTA CCATGGAGCT ATGGACAGTG TCCCTCTCTC TGGAACTACC 
CCACTGCAGA TGCTTTTGGA AATTGGTTTG GACAAACTGA AGAAAGATTA TATCAGCTTT 
TTTGTCAGTC AGGAACTTGC ATCTTTGAAT CATTTGGAAT ACTTCATTTC TCCCTCAGTA 
TCGACACAGG AACAGGTTTG CCGTGTTCAG AAACTCCATC ATATTCTTGA AATCTTAGTC 
ATCTGCATGC TTTTCATTAA ACCTCAGCAT GAGCTTCTCT TCTCTTTAAC ACAATCCTGC 
ATAAAATATT ATAAACAAAA TCCTCTTGAT GAGCAACACA TTTTTCAGTT GCCAGTCAGA 
CCAGCTGCTG TCAAGAATTT GTATCAAAGT GAGAAGCCGC AGAAATGGAG AGTGGAGCTC 
AGCAATAGTC AGAAGAGGGT GAAAACAGTG TGGCAACTGA GTGACAGCTC CCCTGTAGAC 
CATTCCAGTT TCCACAGGCC TGAGTTTCCT GAGCTGACAC TGAATGGTAG TCTGGAAGAA 
AGGACAGCCT TCGTTAACAT GCTTACCTGC AGCCAGGTGC ACTTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080783.3
CDS	41..1831
Translation	MDGGHGGMWSRMNRAAEEFYARLRQEFNEEKKGASKDPFIYEADVQVQLISKGQPSLLKTILNENDSVFLVEKVVLEKEETSQVEELQSEETAISDLSAGENIRPLALPVGRARQLIGLYTMAHNPNMTHLKIKQPVTALPPLWVRCDGSDPEGTCWLGAELITTNDIIAGVILYVLTCKADKNYSEDLENLKTSHKKRHHVSAVTARGFAQYELFKSDDLDDTVAPSQTTVTLDLSWSPVDEMLQTPPLSSTAALNIRVQSGESRGCLSHLHRELKFLLVLADGIRTGVTEWLEPLETKSALEFVQEFLNDLNKLDEFDDSTKKDKQKEAVNHDAAAVVRSMLLTVRGDLDFAEQLWCRMSSSVVSYQDLVKCFTLILQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGAMDSVPLSGTTPLQMLLEIGLDKLKKDYISFFVSQELASLNHLEYFISPSVSTQEQVCRVQKLHHILEILVICMLFIKPQHELLFSLTQSCIKYYKQNPLDEQHIFQLPVRPAAVKNLYQSEKPQKWRVELSNSQKRVKTVWQLSDSSPVDHSSFHRPEFPELTLNGSLEERTAFVNMLTCSQVHFK

Target ORF information:

RefSeq Version	NM_026507.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus zwilch kinetochore protein (Zwilch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026507.4

ORF Insert Sequence:

ATGGACGGAG GCCACGGCGG TATGTGGTCG CGAATGAACC GCGCGGCGGA GGAGTTTTAT 
GCTCGTCTCC GGCAGGAATT TAATGAAGAG AAGAAAGGAG CCTCTAAAGA CCCATTCATC 
TATGAGGCTG ATGTACAAGT GCAGTTGATC AGCAAAGGCC AACCAAGTCT TCTGAAAACT 
ATTCTAAATG AAAATGACTC AGTGTTTCTC GTGGAAAAAG TGGTTTTGGA GAAGGAAGAA 
ACAAGTCAAG TTGAAGAACT GCAGTCTGAA GAAACTGCTA TTTCTGATCT CTCTGCTGGT 
GAAAATATTA GGCCACTGGC CTTACCTGTT GGGAGAGCAA GGCAGTTAAT TGGGCTCTAT 
ACCATGGCTC ACAATCCTAA TATGACTCAT CTGAAGATTA AGCAGCCAGT GACAGCCCTT 
CCTCCTCTTT GGGTGAGATG TGACGGTTCA GATCCTGAAG GTACCTGTTG GCTAGGAGCT 
GAGCTTATCA CAACAAATGA CATCATTGCA GGCGTTATCC TGTATGTGCT CACTTGTAAA 
GCTGATAAAA ATTACTCTGA AGATCTGGAA AATTTAAAAA CTTCACACAA GAAAAGACAT 
CACGTGTCTG CTGTGACAGC CAGAGGCTTT GCCCAGTATG AGCTCTTTAA GTCTGACGAC 
TTGGATGACA CTGTTGCCCC ATCACAAACT ACAGTCACGC TGGACCTTTC CTGGAGCCCT 
GTGGATGAGA TGCTCCAAAC CCCTCCCCTG TCTTCAACTG CAGCTCTGAA TATCAGAGTA 
CAATCAGGAG AGTCCAGAGG TTGTCTGAGT CATCTTCACA GAGAACTGAA ATTTCTCCTT 
GTTTTAGCTG ATGGCATCAG GACTGGTGTA ACTGAATGGC TTGAGCCTCT GGAAACAAAA 
TCTGCTCTTG AATTTGTTCA GGAATTTCTG AATGACTTAA ATAAACTGGA TGAATTTGAT 
GATTCTACAA AAAAAGATAA ACAAAAAGAG GCAGTGAACC ATGACGCAGC TGCAGTTGTC 
AGGTCCATGC TTCTCACCGT GCGGGGGGAT CTCGATTTTG CTGAACAGCT TTGGTGTAGA 
ATGAGCAGCA GTGTGGTTTC CTATCAAGAC TTGGTGAAGT GTTTCACACT CATCCTGCAG 
AGTCTTCAGC GTGGTGATAT ACAGCCATGG TTGCACAGTG GAAGTAACAG TTTACTAAGT 
AAACTCATTC ATCAGTCTTA CCATGGAGCT ATGGACAGTG TCCCTCTCTC TGGAACTACC 
CCACTGCAGA TGCTTTTGGA AATTGGTTTG GACAAACTGA AGAAAGATTA TATCAGCTTT 
TTTGTCAGTC AGGAACTTGC ATCTTTGAAT CATTTGGAAT ACTTCATTTC TCCCTCAGTA 
TCGACACAGG AACAGGTTTG CCGTGTTCAG AAACTCCATC ATATTCTTGA AATCTTAGTC 
ATCTGCATGC TTTTCATTAA ACCTCAGCAT GAGCTTCTCT TCTCTTTAAC ACAATCCTGC 
ATAAAATATT ATAAACAAAA TCCTCTTGAT GAGCAACACA TTTTTCAGTT GCCAGTCAGA 
CCAGCTGCTG TCAAGAATTT GTATCAAAGT GAGAAGCCGC AGAAATGGAG AGTGGAGCTC 
AGCAATAGTC AGAAGAGGGT GAAAACAGTG TGGCAACTGA GTGACAGCTC CCCTGTAGAC 
CATTCCAGTT TCCACAGGCC TGAGTTTCCT GAGCTGACAC TGAATGGTAG TCTGGAAGAA 
AGGACAGCCT TCGTTAACAT GCTTACCTGC AGCCAGGTGC ACTTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

A global genomic transcriptional code associated with CNS-expressed genes.
Experimental cell research312(16)3108-19(2006 Oct)
Bailey PJ,Klos JM,Andersson E,Karl?n M,K?llstr?m M,Ponjavic J,Muhr J,Lenhard B,Sandelin A,Ericson J

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS