Sh3rf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sh3rf1
Entrez Gene ID	59009
Full Name	SH3 domain containing ring finger 1
Synonyms	2200003J05Rik,Posh,R75531,Sh3md2
General protein information	Preferred Names SH3 domain containing ring finger 1 Names E3 ubiquitin-protein ligase SH3RF1 RING-type E3 ubiquitin transferase SH3RF1 SH3 domain-containing RING finger protein 1 SH3 multiple domains 2 SH3 multiple domains protein 2 plenty of SH3 domains plenty of SH3s putative E3 ubiquitin-protein ligase SH3RF1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_021506.2	NP_067481.2	E3 ubiquitin-protein ligase SH3RF1
XM_011242231.1	XP_011240533.1	E3 ubiquitin-protein ligase SH3RF1 isoform X1
XM_011242231.2	XP_011240533.1	E3 ubiquitin-protein ligase SH3RF1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SH3RF1	NP_065921.2
Xenopus tropicalis (tropical clawed frog)	sh3rf1	NP_001015973.1
Mus musculus (house mouse)	Sh3rf1	NP_067481.2
Rattus norvegicus (Norway rat)	Sh3rf1	NP_942059.1
	SH3RF1	XP_001082524.1
Gallus gallus (chicken)	SH3RF1	XP_420402.2
Danio rerio (zebrafish)	sh3rf1	NP_001038952.2
Pan troglodytes (chimpanzee)	SH3RF1	XP_517530.2
Canis lupus familiaris (dog)	SH3RF1	XP_855736.2
Bos taurus (cattle)	SH3RF1	NP_001091534.1

Gene Ontology
Bibliography

Related articles in PubMed

POSH Regulates CD4+ T Cell Differentiation and Survival.
Cunningham CA, Cardwell LN, Guan Y, Teixeiro E, Daniels MA
Journal of immunology (Baltimore, Md. : 1950)196(10)4003-13(2016 05)

A Novel c-Jun N-terminal Kinase (JNK) Signaling Complex Involved in Neuronal Migration during Brain Development.
Zhang F, Yu J, Yang T, Xu D, Chi Z, Xia Y, Xu Z
The Journal of biological chemistry291(22)11466-75(2016 May)

The POSH/JIP-1 scaffold network regulates TCR-mediated JNK1 signals and effector function in CD8(+) T cells.
Cunningham CA, Knudson KM, Peng BJ, Teixeiro E, Daniels MA
European journal of immunology43(12)3361-71(2013 Dec)

POSH localizes activated Rac1 to control the formation of cytoplasmic dilation of the leading process and neuronal migration.
Yang T, Sun Y, Zhang F, Zhu Y, Shi L, Li H, Xu Z
Cell reports2(3)640-51(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that POSH is essential for the integration of cell-type-specific signals that regulate the differentiation, survival, and function of T cells
Title: POSH Regulates CD4+ T Cell Differentiation and Survival.

identify plenty of SH3 (POSH) and JNK-interacting protein 1 (JIP-1) as a multiprotein scaffold network for TCR-mediated JNK1 activation in CD8(+) T cells.
Title: The POSH/JIP-1 scaffold network regulates TCR-mediated JNK1 signals and effector function in CD8(+) T cells.

a mechanism by which POSH regulates the localization of activated Rac1 and F-actin assembly to control PCDLP formation and subsequent somal translocation of migratory neurons.
Title: POSH localizes activated Rac1 to control the formation of cytoplasmic dilation of the leading process and neuronal migration.

An intracellular signaling pathway for process outgrowth inhibition by Nogo66 implicates the POSH complex as a therapeutic target to enhance axon outgrowth and plasticity in the injured central nervous system.
Title: POSH is an intracellular signal transducer for the axon outgrowth inhibitor Nogo66.

Results suggest that the molecular scaffold protein POSH assembles an inhibitory complex that links to the actin-myosin network to regulate neuronal process outgrowth.
Title: The scaffold protein POSH regulates axon outgrowth.

The following Sh3rf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sh3rf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15042	NM_021506.2 Latest version!	Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu69809	XM_011242231.2 Latest version!	Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu69809	XM_011242231.1	Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sh3rf1 ( NM_021506.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067481.2 Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), mRNA.

Price & Availability↑

CloneID	OMu15042
Clone ID Related Accession (Same CDS sequence)	NM_021506.2
Accession Version	NM_021506.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SH3RF1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134339.6, BY741307.1, AC117196.3, AK045470.1 and CO040875.1. On Sep 7, 2006 this sequence version replaced NM_021506.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173185.1, AF030131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCCTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAT ACCTTTTGCA AACGCTGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTC 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCGGG ACCACCTGCA CAAACACATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCGAAA GATCTGCAGA GCTCCCAGTG TGGACAGCAG 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTCTGC GCCGACAGGT GGATGAGAAT TGGTACCACG GGGAAGTCAG CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT CGTGCAGATC ATCAAACCTT TACCTCAGCC CCCGCCTCAG 
TGCAAAGCAC TTTACGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTCGCAAAGG ACGACGTACT GACCGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG AATATTTCCA ATTTCATACG TGGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCC GTGCCAGGAG TGGACACGGC AGAATGCCCC 
TCAGCGACGG CGCAGAGCAC CTCTGCCTCA AAGCACCCCG ACACCAAGAA GAACACCAGG 
AAGCGACACT CCTTCACCTC CCTCACCATG GCCAACAAGT CTTCCCAGGG GTCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCTCCTGTG CTCATCAGTT CCAGCAACCC CACAGCCGCA 
GCCCGCATCA GCGAACTGTC CGGGCTCTCC TGCAGCGCCC CGTCTCAGGT CCATATAAGC 
ACCACTGGGT TAATTGTGAC CCCACCCCCT AGCAGCCCGG TGACAACTGG CCCTGCGTTC 
ACGTTCCCTT CAGATGTCCC CTACCAAGCT GCCCTTGGAA GTATGAATCC TCCACTTCCC 
CCACCCCCTC TCCTGGCGGC CACCGTACTC GCCTCCACCC CGTCAGGCGC TACTGCTGCT 
GTTGCTGCTG CTGCTGCCGC CGCCGCTGCT GGAATGGGAC CCAGGCCTGT GATGGGGTCC 
TCTGAACAGA TTGCACATTT ACGGCCTCAG ACTCGTCCCA GTGTATATGT TGCTATATAT 
CCGTACACTC CCCGGAAGGA AGACGAACTG GAGCTGAGGA AAGGGGAGAT GTTTTTGGTG 
TTTGAGCGTT GCCAGGACGG CTGGTACAAA GGGACATCGA TGCATACCAG CAAGATAGGC 
GTTTTCCCTG GCAACTATGT GGCGCCCGTC ACAAGGGCGG TGACGAATGC CTCCCAAGCT 
AAAGTCTCTA TGTCTACTGC GGGTCAAGCA AGTCGCGGGG TGACCATGGT CAGCCCTTCC 
ACTGCAGGAG GACCTACACA GAAGCCCCAA GGAAACGGCG TGGCCGGAAA TCCCAGCGTC 
GTCCCCACGG CTGTGGTGTC AGCAGCTCAT ATCCAGACAA GTCCTCAGGC TAAGGTCCTG 
CTGCACATGT CTGGGCAGAT GACAGTCAAT CAGGCCCGCA ATGCTGTGAG GACAGTTGCA 
GCACATAGCC AGGAACGCCC CACGGCAGCA GTGACTCCCA TCCAGGTCCA GAATGCCGCC 
TGCCTCGGTC CTGCATCCGT GGGCCTGCCC CATCATTCTC TGGCCTCCCA ACCTCTGCCT 
CCAATGGCGG GTCCTGCTGC CCACGGTGCT GCCGTCAGCA TCAGTCGAAC CAATGCCCCC 
ATGGCCTGCG CTGCAGGGGC TTCTCTGGCC TCCCCAAATA TGACCAGTGC CGTGTTGGAG 
ACAGAGCCCA GTGGTCGCAC AGTGACCATC CTCCCTGGAC TCCCCACATC TCCAGAGAGT 
GCTGCATCAG CGTGTGGGAA CAGTTCAGCT GGGAAACCAG ACAAGGACAG TAAGAAAGAA 
AAAAAGGGCC TACTGAAGCT GCTTTCTGGT GCCTCCACCA AACGCAAGCC CCGAGTCTCC 
CCTCCAGCAT CACCTACCCT GGATGTGGAG CTGGGTGCTG GGGAGGCTCC CTTGCAGGGA 
GCAGTAGGTC CTGAGCTGCC GCTAGGGGGC AGCCACGGCA GAGTGGGGTC ATGCCCCACA 
GATGGTGATG GTCCAGTGGC CGCTGGAACA GCAGCCCTAG CCCAGGATGC CTTCCACCGC 
AAGACAAGCT CCCTGGACTC CGCAGTGCCC ATTGCTCCAC CACCTCGCCA GGCCTGCTCC 
TCCCTGGGCC CAGTCATGAA TGAGGCCCGG CCTGTTGTTT GTGAAAGGCA CAGGGTGGTG 
GTTTCCTACC CTCCTCAGAG TGAGGCCGAA CTTGAACTCA AGGAAGGAGA TATTGTGTTT 
GTTCATAAGA AACGAGAGGA CGGCTGGTTC AAAGGCACGT TACAGAGGAA TGGGAAGACT 
GGCCTTTTCC CAGGGAGCTT TGTGGAAAAC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067481.2
CDS	271..2946
Translation	MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPSNILLVRLLDGIKQRPWKPGPGGGGGTTCTNTLRAQGSTVVNCGSKDLQSSQCGQQPRVQAWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYHGEVSGVHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEGMLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDTAECPSATAQSTSASKHPDTKKNTRKRHSFTSLTMANKSSQGSQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPAFTFPSDVPYQAALGSMNPPLPPPPLLAATVLASTPSGATAAVAAAAAAAAAGMGPRPVMGSSEQIAHLRPQTRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRAVTNASQAKVSMSTAGQASRGVTMVSPSTAGGPTQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKVLLHMSGQMTVNQARNAVRTVAAHSQERPTAAVTPIQVQNAACLGPASVGLPHHSLASQPLPPMAGPAAHGAAVSISRTNAPMACAAGASLASPNMTSAVLETEPSGRTVTILPGLPTSPESAASACGNSSAGKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLDVELGAGEAPLQGAVGPELPLGGSHGRVGSCPTDGDGPVAAGTAALAQDAFHRKTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

Target ORF information:

RefSeq Version	NM_021506.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021506.2

ORF Insert Sequence:

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCCTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAT ACCTTTTGCA AACGCTGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTC 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCGGG ACCACCTGCA CAAACACATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCGAAA GATCTGCAGA GCTCCCAGTG TGGACAGCAG 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTCTGC GCCGACAGGT GGATGAGAAT TGGTACCACG GGGAAGTCAG CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT CGTGCAGATC ATCAAACCTT TACCTCAGCC CCCGCCTCAG 
TGCAAAGCAC TTTACGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTCGCAAAGG ACGACGTACT GACCGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG AATATTTCCA ATTTCATACG TGGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCC GTGCCAGGAG TGGACACGGC AGAATGCCCC 
TCAGCGACGG CGCAGAGCAC CTCTGCCTCA AAGCACCCCG ACACCAAGAA GAACACCAGG 
AAGCGACACT CCTTCACCTC CCTCACCATG GCCAACAAGT CTTCCCAGGG GTCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCTCCTGTG CTCATCAGTT CCAGCAACCC CACAGCCGCA 
GCCCGCATCA GCGAACTGTC CGGGCTCTCC TGCAGCGCCC CGTCTCAGGT CCATATAAGC 
ACCACTGGGT TAATTGTGAC CCCACCCCCT AGCAGCCCGG TGACAACTGG CCCTGCGTTC 
ACGTTCCCTT CAGATGTCCC CTACCAAGCT GCCCTTGGAA GTATGAATCC TCCACTTCCC 
CCACCCCCTC TCCTGGCGGC CACCGTACTC GCCTCCACCC CGTCAGGCGC TACTGCTGCT 
GTTGCTGCTG CTGCTGCCGC CGCCGCTGCT GGAATGGGAC CCAGGCCTGT GATGGGGTCC 
TCTGAACAGA TTGCACATTT ACGGCCTCAG ACTCGTCCCA GTGTATATGT TGCTATATAT 
CCGTACACTC CCCGGAAGGA AGACGAACTG GAGCTGAGGA AAGGGGAGAT GTTTTTGGTG 
TTTGAGCGTT GCCAGGACGG CTGGTACAAA GGGACATCGA TGCATACCAG CAAGATAGGC 
GTTTTCCCTG GCAACTATGT GGCGCCCGTC ACAAGGGCGG TGACGAATGC CTCCCAAGCT 
AAAGTCTCTA TGTCTACTGC GGGTCAAGCA AGTCGCGGGG TGACCATGGT CAGCCCTTCC 
ACTGCAGGAG GACCTACACA GAAGCCCCAA GGAAACGGCG TGGCCGGAAA TCCCAGCGTC 
GTCCCCACGG CTGTGGTGTC AGCAGCTCAT ATCCAGACAA GTCCTCAGGC TAAGGTCCTG 
CTGCACATGT CTGGGCAGAT GACAGTCAAT CAGGCCCGCA ATGCTGTGAG GACAGTTGCA 
GCACATAGCC AGGAACGCCC CACGGCAGCA GTGACTCCCA TCCAGGTCCA GAATGCCGCC 
TGCCTCGGTC CTGCATCCGT GGGCCTGCCC CATCATTCTC TGGCCTCCCA ACCTCTGCCT 
CCAATGGCGG GTCCTGCTGC CCACGGTGCT GCCGTCAGCA TCAGTCGAAC CAATGCCCCC 
ATGGCCTGCG CTGCAGGGGC TTCTCTGGCC TCCCCAAATA TGACCAGTGC CGTGTTGGAG 
ACAGAGCCCA GTGGTCGCAC AGTGACCATC CTCCCTGGAC TCCCCACATC TCCAGAGAGT 
GCTGCATCAG CGTGTGGGAA CAGTTCAGCT GGGAAACCAG ACAAGGACAG TAAGAAAGAA 
AAAAAGGGCC TACTGAAGCT GCTTTCTGGT GCCTCCACCA AACGCAAGCC CCGAGTCTCC 
CCTCCAGCAT CACCTACCCT GGATGTGGAG CTGGGTGCTG GGGAGGCTCC CTTGCAGGGA 
GCAGTAGGTC CTGAGCTGCC GCTAGGGGGC AGCCACGGCA GAGTGGGGTC ATGCCCCACA 
GATGGTGATG GTCCAGTGGC CGCTGGAACA GCAGCCCTAG CCCAGGATGC CTTCCACCGC 
AAGACAAGCT CCCTGGACTC CGCAGTGCCC ATTGCTCCAC CACCTCGCCA GGCCTGCTCC 
TCCCTGGGCC CAGTCATGAA TGAGGCCCGG CCTGTTGTTT GTGAAAGGCA CAGGGTGGTG 
GTTTCCTACC CTCCTCAGAG TGAGGCCGAA CTTGAACTCA AGGAAGGAGA TATTGTGTTT 
GTTCATAAGA AACGAGAGGA CGGCTGGTTC AAAGGCACGT TACAGAGGAA TGGGAAGACT 
GGCCTTTTCC CAGGGAGCTT TGTGGAAAAC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3rf1 ( XM_011242231.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240533.1 Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69809
Clone ID Related Accession (Same CDS sequence)	XM_011242231.1 , XM_011242231.2
Accession Version	XM_011242231.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SH3RF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCCTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAT ACCTTTTGCA AACGCTGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTC 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCGGG ACCACCTGCA CAAACACATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCGAAA GATCTGCAGA GCTCCCAGTG TGGACAGCAG 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTCTGC GCCGACAGGT GGATGAGAAT TGGTACCACG GGGAAGTCAG CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT CGTGCAGATC ATCAAACCTT TACCTCAGCC CCCGCCTCAG 
TGCAAAGCAC TTTACGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTCGCAAAGG ACGACGTACT GACCGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG AATATTTCCA ATTTCATACG TGGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCC GTGCCAGGAG TGGACACGGC AGAATGCCCC 
TCAGCGACGG CGCAGAGCAC CTCTGCCTCA AAGCACCCCG ACACCAAGAA GAACACCAGG 
AAGCGACACT CCTTCACCTC CCTCACCATG GCCAACAAGT CTTCCCAGGG GTCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCTCCTGTG CTCATCAGTT CCAGCAACCC CACAGCCGCA 
GCCCGCATCA GCGAACTGTC CGGGCTCTCC TGCAGCGCCC CGTCTCAGGT CCATATAAGC 
ACCACTGGGT TAATTGTGAC CCCACCCCCT AGCAGCCCGG TGACAACTGG CCCTGCGTTC 
ACGTTCCCTT CAGATGTCCC CTACCAAGCT GCCCTTGGAA GTATGAATCC TCCACTTCCC 
CCACCCCCTC TCCTGGCGGC CACCGTACTC GCCTCCACCC CGTCAGGCGC TACTGCTGCT 
GTTGCTGCTG CTGCTGCCGC CGCCGCTGCT GGAATGGGAC CCAGGCCTGT GATGGGGTCC 
TCTGAACAGA TTGCACATTT ACGGCCTCAG ACTCGTCCCA GTGTATATGT TGCTATATAT 
CCGTACACTC CCCGGAAGGA AGACGAACTG GAGCTGAGGA AAGGGGAGAT GTTTTTGGTG 
TTTGAGCGTT GCCAGGACGG CTGGTACAAA GGGACATCGA TGCATACCAG CAAGATAGGC 
GTTTTCCCTG GCAACTATGT GGCGCCCGTC ACAAGGGCGG TGACGAATGC CTCCCAAGCT 
AAAGTCTCTA TGTCTACTGC GGGTCAAGCA AGTCGCGGGG TGACCATGGT CAGCCCTTCC 
ACTGCAGGAG GACCTACACA GAAGCCCCAA GGAAACGGCG TGGCCGGAAA TCCCAGCGTC 
GTCCCCACGG CTGTGGTGTC AGCAGCTCAT ATCCAGACAA GTCCTCAGGC TAAGGTCCTG 
CTGCACATGT CTGGGCAGAT GACAGTCAAT CAGGCCCGCA ATGCTGTGAG GACAGTTGCA 
GCACATAGCC AGGAACGCCC CACGGCAGCA GTGACTCCCA TCCAGGTCCA GAATGCCGCC 
TGCCTCGGTC CTGCATCCGT GGGCCTGCCC CATCATTCTC TGGCCTCCCA ACCTCTGCCT 
CCAATGGCGG GTCCTGCTGC CCACGGTGCT GCCGTCAGCA TCAGTCGAAC CAATGCCCCC 
ATGGCCTGCG CTGCAGGGGC TTCTCTGGCC TCCCCAAATA TGACCAGTGC CGTGTTGGAG 
ACAGAGCCCA GTGGTCGCAC AGTGACCATC CTCCCTGGAC TCCCCACATC TCCAGAGAGT 
GCTGCATCAG CGTGTGGGAA CAGTTCAGCT GGGAAACCAG ACAAGGACAG TAAGAAAGAA 
AAAAAGGGCC TACTGAAGCT GCTTTCTGGT GCCTCCACCA AACGCAAGCC CCGAGTCTCC 
CCTCCAGCAT CACCTACCCT GGATGTGGAG CTGGGTGCTG GGGAGGCTCC CTTGCAGGGA 
GCAGTAGGTC CTGAGCTGCC GCTAGGGGGC AGCCACGGCA GAGTGGGGTC ATGCCCCACA 
GATGGTGATG GTCCAGTGGC CGCTGGAACA GCAGCCCTAG CCCAGGATGC CTTCCACCGC 
AAGACAAGCT CCCTGGACTC CGCAGTGCCC ATTGCTCCAC CACCTCGCCA GGCCTGCTCC 
TCCCTGGGCC CAGTCATGAA TGAGGCCCGG CCTGTTGTTT GTGAAAGGAC CACAGCTGAT 
GGTCTGCTGT TGCCCACATT CAGAAATGTC TGCCATCCTC AGTTAAACCT CTCTGGCAGC 
ACCCTCCCAG GCATGCCCAG AGGTGTCTTC TACCTGACTC TAGATCCAGT CAAGATGCCA 
ATGTGGGTTA ACCGTCATTT CATGGTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240533.1
CDS	198..2870
Translation	MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPSNILLVRLLDGIKQRPWKPGPGGGGGTTCTNTLRAQGSTVVNCGSKDLQSSQCGQQPRVQAWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYHGEVSGVHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEGMLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDTAECPSATAQSTSASKHPDTKKNTRKRHSFTSLTMANKSSQGSQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPAFTFPSDVPYQAALGSMNPPLPPPPLLAATVLASTPSGATAAVAAAAAAAAAGMGPRPVMGSSEQIAHLRPQTRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRAVTNASQAKVSMSTAGQASRGVTMVSPSTAGGPTQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKVLLHMSGQMTVNQARNAVRTVAAHSQERPTAAVTPIQVQNAACLGPASVGLPHHSLASQPLPPMAGPAAHGAAVSISRTNAPMACAAGASLASPNMTSAVLETEPSGRTVTILPGLPTSPESAASACGNSSAGKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLDVELGAGEAPLQGAVGPELPLGGSHGRVGSCPTDGDGPVAAGTAALAQDAFHRKTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVCERTTADGLLLPTFRNVCHPQLNLSGSTLPGMPRGVFYLTLDPVKMPMWVNRHFMVL

Target ORF information:

RefSeq Version	XM_011242231.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242231.1

ORF Insert Sequence:

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCCTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAT ACCTTTTGCA AACGCTGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTC 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCGGG ACCACCTGCA CAAACACATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCGAAA GATCTGCAGA GCTCCCAGTG TGGACAGCAG 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTCTGC GCCGACAGGT GGATGAGAAT TGGTACCACG GGGAAGTCAG CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT CGTGCAGATC ATCAAACCTT TACCTCAGCC CCCGCCTCAG 
TGCAAAGCAC TTTACGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTCGCAAAGG ACGACGTACT GACCGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG AATATTTCCA ATTTCATACG TGGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCC GTGCCAGGAG TGGACACGGC AGAATGCCCC 
TCAGCGACGG CGCAGAGCAC CTCTGCCTCA AAGCACCCCG ACACCAAGAA GAACACCAGG 
AAGCGACACT CCTTCACCTC CCTCACCATG GCCAACAAGT CTTCCCAGGG GTCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCTCCTGTG CTCATCAGTT CCAGCAACCC CACAGCCGCA 
GCCCGCATCA GCGAACTGTC CGGGCTCTCC TGCAGCGCCC CGTCTCAGGT CCATATAAGC 
ACCACTGGGT TAATTGTGAC CCCACCCCCT AGCAGCCCGG TGACAACTGG CCCTGCGTTC 
ACGTTCCCTT CAGATGTCCC CTACCAAGCT GCCCTTGGAA GTATGAATCC TCCACTTCCC 
CCACCCCCTC TCCTGGCGGC CACCGTACTC GCCTCCACCC CGTCAGGCGC TACTGCTGCT 
GTTGCTGCTG CTGCTGCCGC CGCCGCTGCT GGAATGGGAC CCAGGCCTGT GATGGGGTCC 
TCTGAACAGA TTGCACATTT ACGGCCTCAG ACTCGTCCCA GTGTATATGT TGCTATATAT 
CCGTACACTC CCCGGAAGGA AGACGAACTG GAGCTGAGGA AAGGGGAGAT GTTTTTGGTG 
TTTGAGCGTT GCCAGGACGG CTGGTACAAA GGGACATCGA TGCATACCAG CAAGATAGGC 
GTTTTCCCTG GCAACTATGT GGCGCCCGTC ACAAGGGCGG TGACGAATGC CTCCCAAGCT 
AAAGTCTCTA TGTCTACTGC GGGTCAAGCA AGTCGCGGGG TGACCATGGT CAGCCCTTCC 
ACTGCAGGAG GACCTACACA GAAGCCCCAA GGAAACGGCG TGGCCGGAAA TCCCAGCGTC 
GTCCCCACGG CTGTGGTGTC AGCAGCTCAT ATCCAGACAA GTCCTCAGGC TAAGGTCCTG 
CTGCACATGT CTGGGCAGAT GACAGTCAAT CAGGCCCGCA ATGCTGTGAG GACAGTTGCA 
GCACATAGCC AGGAACGCCC CACGGCAGCA GTGACTCCCA TCCAGGTCCA GAATGCCGCC 
TGCCTCGGTC CTGCATCCGT GGGCCTGCCC CATCATTCTC TGGCCTCCCA ACCTCTGCCT 
CCAATGGCGG GTCCTGCTGC CCACGGTGCT GCCGTCAGCA TCAGTCGAAC CAATGCCCCC 
ATGGCCTGCG CTGCAGGGGC TTCTCTGGCC TCCCCAAATA TGACCAGTGC CGTGTTGGAG 
ACAGAGCCCA GTGGTCGCAC AGTGACCATC CTCCCTGGAC TCCCCACATC TCCAGAGAGT 
GCTGCATCAG CGTGTGGGAA CAGTTCAGCT GGGAAACCAG ACAAGGACAG TAAGAAAGAA 
AAAAAGGGCC TACTGAAGCT GCTTTCTGGT GCCTCCACCA AACGCAAGCC CCGAGTCTCC 
CCTCCAGCAT CACCTACCCT GGATGTGGAG CTGGGTGCTG GGGAGGCTCC CTTGCAGGGA 
GCAGTAGGTC CTGAGCTGCC GCTAGGGGGC AGCCACGGCA GAGTGGGGTC ATGCCCCACA 
GATGGTGATG GTCCAGTGGC CGCTGGAACA GCAGCCCTAG CCCAGGATGC CTTCCACCGC 
AAGACAAGCT CCCTGGACTC CGCAGTGCCC ATTGCTCCAC CACCTCGCCA GGCCTGCTCC 
TCCCTGGGCC CAGTCATGAA TGAGGCCCGG CCTGTTGTTT GTGAAAGGAC CACAGCTGAT 
GGTCTGCTGT TGCCCACATT CAGAAATGTC TGCCATCCTC AGTTAAACCT CTCTGGCAGC 
ACCCTCCCAG GCATGCCCAG AGGTGTCTTC TACCTGACTC TAGATCCAGT CAAGATGCCA 
ATGTGGGTTA ACCGTCATTT CATGGTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3rf1 ( XM_011242231.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240533.1 Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.

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CloneID	OMu69809
Clone ID Related Accession (Same CDS sequence)	XM_011242231.1 , XM_011242231.2
Accession Version	XM_011242231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SH3RF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCCTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAT ACCTTTTGCA AACGCTGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTC 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCGGG ACCACCTGCA CAAACACATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCGAAA GATCTGCAGA GCTCCCAGTG TGGACAGCAG 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTCTGC GCCGACAGGT GGATGAGAAT TGGTACCACG GGGAAGTCAG CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT CGTGCAGATC ATCAAACCTT TACCTCAGCC CCCGCCTCAG 
TGCAAAGCAC TTTACGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTCGCAAAGG ACGACGTACT GACCGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG AATATTTCCA ATTTCATACG TGGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCC GTGCCAGGAG TGGACACGGC AGAATGCCCC 
TCAGCGACGG CGCAGAGCAC CTCTGCCTCA AAGCACCCCG ACACCAAGAA GAACACCAGG 
AAGCGACACT CCTTCACCTC CCTCACCATG GCCAACAAGT CTTCCCAGGG GTCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCTCCTGTG CTCATCAGTT CCAGCAACCC CACAGCCGCA 
GCCCGCATCA GCGAACTGTC CGGGCTCTCC TGCAGCGCCC CGTCTCAGGT CCATATAAGC 
ACCACTGGGT TAATTGTGAC CCCACCCCCT AGCAGCCCGG TGACAACTGG CCCTGCGTTC 
ACGTTCCCTT CAGATGTCCC CTACCAAGCT GCCCTTGGAA GTATGAATCC TCCACTTCCC 
CCACCCCCTC TCCTGGCGGC CACCGTACTC GCCTCCACCC CGTCAGGCGC TACTGCTGCT 
GTTGCTGCTG CTGCTGCCGC CGCCGCTGCT GGAATGGGAC CCAGGCCTGT GATGGGGTCC 
TCTGAACAGA TTGCACATTT ACGGCCTCAG ACTCGTCCCA GTGTATATGT TGCTATATAT 
CCGTACACTC CCCGGAAGGA AGACGAACTG GAGCTGAGGA AAGGGGAGAT GTTTTTGGTG 
TTTGAGCGTT GCCAGGACGG CTGGTACAAA GGGACATCGA TGCATACCAG CAAGATAGGC 
GTTTTCCCTG GCAACTATGT GGCGCCCGTC ACAAGGGCGG TGACGAATGC CTCCCAAGCT 
AAAGTCTCTA TGTCTACTGC GGGTCAAGCA AGTCGCGGGG TGACCATGGT CAGCCCTTCC 
ACTGCAGGAG GACCTACACA GAAGCCCCAA GGAAACGGCG TGGCCGGAAA TCCCAGCGTC 
GTCCCCACGG CTGTGGTGTC AGCAGCTCAT ATCCAGACAA GTCCTCAGGC TAAGGTCCTG 
CTGCACATGT CTGGGCAGAT GACAGTCAAT CAGGCCCGCA ATGCTGTGAG GACAGTTGCA 
GCACATAGCC AGGAACGCCC CACGGCAGCA GTGACTCCCA TCCAGGTCCA GAATGCCGCC 
TGCCTCGGTC CTGCATCCGT GGGCCTGCCC CATCATTCTC TGGCCTCCCA ACCTCTGCCT 
CCAATGGCGG GTCCTGCTGC CCACGGTGCT GCCGTCAGCA TCAGTCGAAC CAATGCCCCC 
ATGGCCTGCG CTGCAGGGGC TTCTCTGGCC TCCCCAAATA TGACCAGTGC CGTGTTGGAG 
ACAGAGCCCA GTGGTCGCAC AGTGACCATC CTCCCTGGAC TCCCCACATC TCCAGAGAGT 
GCTGCATCAG CGTGTGGGAA CAGTTCAGCT GGGAAACCAG ACAAGGACAG TAAGAAAGAA 
AAAAAGGGCC TACTGAAGCT GCTTTCTGGT GCCTCCACCA AACGCAAGCC CCGAGTCTCC 
CCTCCAGCAT CACCTACCCT GGATGTGGAG CTGGGTGCTG GGGAGGCTCC CTTGCAGGGA 
GCAGTAGGTC CTGAGCTGCC GCTAGGGGGC AGCCACGGCA GAGTGGGGTC ATGCCCCACA 
GATGGTGATG GTCCAGTGGC CGCTGGAACA GCAGCCCTAG CCCAGGATGC CTTCCACCGC 
AAGACAAGCT CCCTGGACTC CGCAGTGCCC ATTGCTCCAC CACCTCGCCA GGCCTGCTCC 
TCCCTGGGCC CAGTCATGAA TGAGGCCCGG CCTGTTGTTT GTGAAAGGAC CACAGCTGAT 
GGTCTGCTGT TGCCCACATT CAGAAATGTC TGCCATCCTC AGTTAAACCT CTCTGGCAGC 
ACCCTCCCAG GCATGCCCAG AGGTGTCTTC TACCTGACTC TAGATCCAGT CAAGATGCCA 
ATGTGGGTTA ACCGTCATTT CATGGTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240533.1
CDS	359..3031
Translation	MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPSNILLVRLLDGIKQRPWKPGPGGGGGTTCTNTLRAQGSTVVNCGSKDLQSSQCGQQPRVQAWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYHGEVSGVHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEGMLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDTAECPSATAQSTSASKHPDTKKNTRKRHSFTSLTMANKSSQGSQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPAFTFPSDVPYQAALGSMNPPLPPPPLLAATVLASTPSGATAAVAAAAAAAAAGMGPRPVMGSSEQIAHLRPQTRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRAVTNASQAKVSMSTAGQASRGVTMVSPSTAGGPTQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKVLLHMSGQMTVNQARNAVRTVAAHSQERPTAAVTPIQVQNAACLGPASVGLPHHSLASQPLPPMAGPAAHGAAVSISRTNAPMACAAGASLASPNMTSAVLETEPSGRTVTILPGLPTSPESAASACGNSSAGKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLDVELGAGEAPLQGAVGPELPLGGSHGRVGSCPTDGDGPVAAGTAALAQDAFHRKTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVCERTTADGLLLPTFRNVCHPQLNLSGSTLPGMPRGVFYLTLDPVKMPMWVNRHFMVL

Target ORF information:

RefSeq Version	XM_011242231.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242231.2

ORF Insert Sequence:

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCCTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAT ACCTTTTGCA AACGCTGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTC 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCGGG ACCACCTGCA CAAACACATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCGAAA GATCTGCAGA GCTCCCAGTG TGGACAGCAG 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTCTGC GCCGACAGGT GGATGAGAAT TGGTACCACG GGGAAGTCAG CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT CGTGCAGATC ATCAAACCTT TACCTCAGCC CCCGCCTCAG 
TGCAAAGCAC TTTACGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTCGCAAAGG ACGACGTACT GACCGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG AATATTTCCA ATTTCATACG TGGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCC GTGCCAGGAG TGGACACGGC AGAATGCCCC 
TCAGCGACGG CGCAGAGCAC CTCTGCCTCA AAGCACCCCG ACACCAAGAA GAACACCAGG 
AAGCGACACT CCTTCACCTC CCTCACCATG GCCAACAAGT CTTCCCAGGG GTCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCTCCTGTG CTCATCAGTT CCAGCAACCC CACAGCCGCA 
GCCCGCATCA GCGAACTGTC CGGGCTCTCC TGCAGCGCCC CGTCTCAGGT CCATATAAGC 
ACCACTGGGT TAATTGTGAC CCCACCCCCT AGCAGCCCGG TGACAACTGG CCCTGCGTTC 
ACGTTCCCTT CAGATGTCCC CTACCAAGCT GCCCTTGGAA GTATGAATCC TCCACTTCCC 
CCACCCCCTC TCCTGGCGGC CACCGTACTC GCCTCCACCC CGTCAGGCGC TACTGCTGCT 
GTTGCTGCTG CTGCTGCCGC CGCCGCTGCT GGAATGGGAC CCAGGCCTGT GATGGGGTCC 
TCTGAACAGA TTGCACATTT ACGGCCTCAG ACTCGTCCCA GTGTATATGT TGCTATATAT 
CCGTACACTC CCCGGAAGGA AGACGAACTG GAGCTGAGGA AAGGGGAGAT GTTTTTGGTG 
TTTGAGCGTT GCCAGGACGG CTGGTACAAA GGGACATCGA TGCATACCAG CAAGATAGGC 
GTTTTCCCTG GCAACTATGT GGCGCCCGTC ACAAGGGCGG TGACGAATGC CTCCCAAGCT 
AAAGTCTCTA TGTCTACTGC GGGTCAAGCA AGTCGCGGGG TGACCATGGT CAGCCCTTCC 
ACTGCAGGAG GACCTACACA GAAGCCCCAA GGAAACGGCG TGGCCGGAAA TCCCAGCGTC 
GTCCCCACGG CTGTGGTGTC AGCAGCTCAT ATCCAGACAA GTCCTCAGGC TAAGGTCCTG 
CTGCACATGT CTGGGCAGAT GACAGTCAAT CAGGCCCGCA ATGCTGTGAG GACAGTTGCA 
GCACATAGCC AGGAACGCCC CACGGCAGCA GTGACTCCCA TCCAGGTCCA GAATGCCGCC 
TGCCTCGGTC CTGCATCCGT GGGCCTGCCC CATCATTCTC TGGCCTCCCA ACCTCTGCCT 
CCAATGGCGG GTCCTGCTGC CCACGGTGCT GCCGTCAGCA TCAGTCGAAC CAATGCCCCC 
ATGGCCTGCG CTGCAGGGGC TTCTCTGGCC TCCCCAAATA TGACCAGTGC CGTGTTGGAG 
ACAGAGCCCA GTGGTCGCAC AGTGACCATC CTCCCTGGAC TCCCCACATC TCCAGAGAGT 
GCTGCATCAG CGTGTGGGAA CAGTTCAGCT GGGAAACCAG ACAAGGACAG TAAGAAAGAA 
AAAAAGGGCC TACTGAAGCT GCTTTCTGGT GCCTCCACCA AACGCAAGCC CCGAGTCTCC 
CCTCCAGCAT CACCTACCCT GGATGTGGAG CTGGGTGCTG GGGAGGCTCC CTTGCAGGGA 
GCAGTAGGTC CTGAGCTGCC GCTAGGGGGC AGCCACGGCA GAGTGGGGTC ATGCCCCACA 
GATGGTGATG GTCCAGTGGC CGCTGGAACA GCAGCCCTAG CCCAGGATGC CTTCCACCGC 
AAGACAAGCT CCCTGGACTC CGCAGTGCCC ATTGCTCCAC CACCTCGCCA GGCCTGCTCC 
TCCCTGGGCC CAGTCATGAA TGAGGCCCGG CCTGTTGTTT GTGAAAGGAC CACAGCTGAT 
GGTCTGCTGT TGCCCACATT CAGAAATGTC TGCCATCCTC AGTTAAACCT CTCTGGCAGC 
ACCCTCCCAG GCATGCCCAG AGGTGTCTTC TACCTGACTC TAGATCCAGT CAAGATGCCA 
ATGTGGGTTA ACCGTCATTT CATGGTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

POSH Regulates CD4+ T Cell Differentiation and Survival.
Journal of immunology (Baltimore, Md. : 1950)196(10)4003-13(2016 05)
Cunningham CA,Cardwell LN,Guan Y,Teixeiro E,Daniels MA

A Novel c-Jun N-terminal Kinase (JNK) Signaling Complex Involved in Neuronal Migration during Brain Development.
The Journal of biological chemistry291(22)11466-75(2016 May)
Zhang F,Yu J,Yang T,Xu D,Chi Z,Xia Y,Xu Z

The POSH/JIP-1 scaffold network regulates TCR-mediated JNK1 signals and effector function in CD8(+) T cells.
European journal of immunology43(12)3361-71(2013 Dec)
Cunningham CA,Knudson KM,Peng BJ,Teixeiro E,Daniels MA

POSH localizes activated Rac1 to control the formation of cytoplasmic dilation of the leading process and neuronal migration.
Cell reports2(3)640-51(2012 Sep)
Yang T,Sun Y,Zhang F,Zhu Y,Shi L,Li H,Xu Z