Noa1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Noa1
Entrez Gene ID	56412
Full Name	nitric oxide associated 1
Synonyms	2610024G14Rik,AU022345,AtNOS1,mAtNOS1
General protein information	Preferred Names nitric oxide associated 1 Names nitric oxide-associated protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006535153.4	XP_006535216.1	nitric oxide-associated protein 1 isoform X1
XM_011249546.3	XP_011247848.1	nitric oxide-associated protein 1 isoform X2
XM_011249546.2	XP_011247848.1	nitric oxide-associated protein 1 isoform X2
XM_006535153.3	XP_006535216.1	nitric oxide-associated protein 1 isoform X1
NM_019836.3	NP_062810.1	nitric oxide-associated protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NOA1	XP_854207.1
Bos taurus (cattle)	NOA1	NP_001033277.1
Mus musculus (house mouse)	Noa1	NP_062810.1
Danio rerio (zebrafish)	LOC101883563	XP_005174712.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010271	XP_319462.4
Caenorhabditis elegans (roundworm)	Y48E1B.2	NP_496845.1
Danio rerio (zebrafish)	noa1	NP_001107900.1
Homo sapiens (human)	NOA1	NP_115689.1
Gallus gallus (chicken)	NOA1	XP_420579.4
Drosophila melanogaster (fruit fly)	CG10914	NP_611297.3
Xenopus tropicalis (tropical clawed frog)	noa1	XP_002937599.2
Pan troglodytes (chimpanzee)	NOA1	XP_001136575.2
	C4orf14	XP_001082521.1
Rattus norvegicus (Norway rat)	Noa1	NP_001006960.1

Gene Ontology
Bibliography

Related articles in PubMed

NOA1, a novel ClpXP substrate, takes an unexpected nuclear detour prior to mitochondrial import.
Al-Furoukh N, Kardon JR, Kr?ger M, Szibor M, Baker TA, Braun T
PloS one9(7)e103141(2014)

Binding to G-quadruplex RNA activates the mitochondrial GTPase NOA1.
Al-Furoukh N, Goffart S, Szibor M, Wanrooij S, Braun T
Biochimica et biophysica acta1833(12)2933-2942(2013 Dec)

Nitric oxide-associated protein 1 (NOA1) is necessary for oxygen-dependent regulation of mitochondrial respiratory complexes.
Heidler J, Al-Furoukh N, Kukat C, Salwig I, Ingelmann ME, Seibel P, Kr?ger M, Holtz J, Wittig I, Braun T, Szibor M
The Journal of biological chemistry286(37)32086-93(2011 Sep)

NOA1 is an essential GTPase required for mitochondrial protein synthesis.
Kolanczyk M, Pech M, Zemojtel T, Yamamoto H, Mikula I, Calvaruso MA, van den Brand M, Richter R, Fischer B, Ritz A, Kossler N, Thurisch B, Spoerle R, Smeitink J, Kornak U, Chan D, Vingron M, Martasek P, Lightowlers RN, Nijtmans L, Schuelke M, Nierhaus KH, Mundlos S
Molecular biology of the cell22(1)1-11(2011 Jan)

mAtNOS1 induces apoptosis of human mammary adenocarcinoma cells.
Parihar A, Parihar MS, Chen Z, Ghafourifar P
Life sciences82(21-22)1077-82(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results uncovered an unexpected, mandatory detour of NOA1 through the nucleolus before uptake into mitochondria.
Title: NOA1, a novel ClpXP substrate, takes an unexpected nuclear detour prior to mitochondrial import.

NOA1 binds specifically to oligonucleotides that fold into guanine tetrads (G-quadruplexes).
Title: Binding to G-quadruplex RNA activates the mitochondrial GTPase NOA1.

data show that NOA1 is required for mitochondrial protein synthesis, likely due to its yet unidentified role in mitoribosomal biogenesis; NOA1 is required for such basal mitochondrial functions as adenosine triphosphate (ATP) synthesis and apoptosis
Title: NOA1 is an essential GTPase required for mitochondrial protein synthesis.

The following Noa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Noa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu35620	XM_006535153.4 Latest version!	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu69650	XM_011249546.3 Latest version!	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu13630	NM_019836.3 Latest version!	Mus musculus nitric oxide associated 1 (Noa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$405.30 $579.00
View Old Accession Versions >>
OMu69650	XM_011249546.2	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu35620	XM_006535153.3	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Noa1 ( XM_006535153.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535216.1 Mus musculus nitric oxide associated 1 (Noa1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35620
Clone ID Related Accession (Same CDS sequence)	XM_006535153.4 , XM_006535153.3
Accession Version	XM_006535153.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nitric oxide-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535153.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTGG AAATCAGTCA GCCTGGTTCA CAGTTGTGGC TTCCAACTTC 
CTTCCTGTTC ATATTACTTC CTTGGACAAG GCAGATGCTC TGTATGAGAA ACATGCAGGT 
CACGAGCTAC TGCTGGTTCC AATGGGTGGA AAAGAACGAA TGGCCCAGTT TCCCCCTCTT 
GTTGCTGAAG ACATTACATT AAAGGGAGGT GGAAAATTTG AAGCAGTAGC TGATATCAAG 
TTCTCCTCTG CAGGTTGGGT TGCAGTAACA CCTTATTCTG AGGGCACGCT GCATCTCCGA 
GGTCACACAC CTGAAGGGAC TGCTCTGACT GTCCACCCCC CTGTCTTGCC ATATATCGTT 
AATGTCAAAG GACAGCGCAT GAAGAAAAGT GTGGCCTATA AAACCAAGAA GCCTCCGTCC 
CTGGTGCACA ACCTGAAGAA GCACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535216.1
CDS	358..2307
Translation	MLPARLACGLLCGLRRGPAPAAACYGPARWLLEGKCEVPIRQRASSLGRRVPPSSTATEDYAEGPDTEERFLFPEYVPERTPEEQVRELQELRELQQLQQEKERERLQQREERLQQKLRAGFRTLPVPEFPDASVPPSGIYCSGCGAELHCQHPGLPGYLPEEKFRDAAQAEGGPARTVCQRCWLLVHHGRALRLQVSRDQYLELVSAALRRPGPALVLYMVNLLDLPDALLPDLPKLVGPKQLIVLGNKVDLLPQDAPGYLKRLRKRLWDDCIRAGLVVAPGHQGPQYPAGDEPLEEIKNQNPSSRSRTVVKDVRLISAKTGYGVEEMISALQRSWRYRGDVYLVGTTNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRQRRLQEDATKAEEDLSEEEQSQLNQLKKHGYIVGRVGRTFSYSREQDEVPFEFDADSLAFDMGSEPVVSVCKSTKQIELTPEDVKDAHWFYDTPGITKESCGNQSAWFTVVASNFLPVHITSLDKADALYEKHAGHELLLVPMGGKERMAQFPPLVAEDITLKGGGKFEAVADIKFSSAGWVAVTPYSEGTLHLRGHTPEGTALTVHPPVLPYIVNVKGQRMKKSVAYKTKKPPSLVHNLKKHR

Target ORF information:

RefSeq Version	XM_006535153.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535153.4

ORF Insert Sequence:

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTGG AAATCAGTCA GCCTGGTTCA CAGTTGTGGC TTCCAACTTC 
CTTCCTGTTC ATATTACTTC CTTGGACAAG GCAGATGCTC TGTATGAGAA ACATGCAGGT 
CACGAGCTAC TGCTGGTTCC AATGGGTGGA AAAGAACGAA TGGCCCAGTT TCCCCCTCTT 
GTTGCTGAAG ACATTACATT AAAGGGAGGT GGAAAATTTG AAGCAGTAGC TGATATCAAG 
TTCTCCTCTG CAGGTTGGGT TGCAGTAACA CCTTATTCTG AGGGCACGCT GCATCTCCGA 
GGTCACACAC CTGAAGGGAC TGCTCTGACT GTCCACCCCC CTGTCTTGCC ATATATCGTT 
AATGTCAAAG GACAGCGCAT GAAGAAAAGT GTGGCCTATA AAACCAAGAA GCCTCCGTCC 
CTGGTGCACA ACCTGAAGAA GCACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Noa1 ( XM_011249546.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247848.1 Mus musculus nitric oxide associated 1 (Noa1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69650
Clone ID Related Accession (Same CDS sequence)	XM_011249546.3 , XM_011249546.2
Accession Version	XM_011249546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nitric oxide-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTAT TTTAAATCTT CTAACAGAAA AAGAAATAAA TACTGTTTTG 
CCGACACATT CTATTATCCC AAGAACCTTT GTCCTCAAGC CAGGAATGGT TTTATTTTTG 
GGTGGGATAG CCCGAATAGA TTTCCTACAG GTGGGGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247848.1
CDS	107..1768
Translation	MLPARLACGLLCGLRRGPAPAAACYGPARWLLEGKCEVPIRQRASSLGRRVPPSSTATEDYAEGPDTEERFLFPEYVPERTPEEQVRELQELRELQQLQQEKERERLQQREERLQQKLRAGFRTLPVPEFPDASVPPSGIYCSGCGAELHCQHPGLPGYLPEEKFRDAAQAEGGPARTVCQRCWLLVHHGRALRLQVSRDQYLELVSAALRRPGPALVLYMVNLLDLPDALLPDLPKLVGPKQLIVLGNKVDLLPQDAPGYLKRLRKRLWDDCIRAGLVVAPGHQGPQYPAGDEPLEEIKNQNPSSRSRTVVKDVRLISAKTGYGVEEMISALQRSWRYRGDVYLVGTTNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRQRRLQEDATKAEEDLSEEEQSQLNQLKKHGYIVGRVGRTFSYSREQDEVPFEFDADSLAFDMGSEPVVSVCKSTKQIELTPEDVKDAHWFYDTPGITKESCILNLLTEKEINTVLPTHSIIPRTFVLKPGMVLFLGGIARIDFLQVGL

Target ORF information:

RefSeq Version	XM_011249546.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249546.3

ORF Insert Sequence:

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTAT TTTAAATCTT CTAACAGAAA AAGAAATAAA TACTGTTTTG 
CCGACACATT CTATTATCCC AAGAACCTTT GTCCTCAAGC CAGGAATGGT TTTATTTTTG 
GGTGGGATAG CCCGAATAGA TTTCCTACAG GTGGGGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Noa1 ( NM_019836.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062810.1 Mus musculus nitric oxide associated 1 (Noa1), mRNA.

Price & Availability↑

CloneID	OMu13630
Clone ID Related Accession (Same CDS sequence)	NM_019836.3
Accession Version	NM_019836.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nitric oxide-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC141469.4, AK017793.1 and AA681543.1. On Jul 21, 2009 this sequence version replaced NM_019836.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK157246.1, AK011533.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTAT TTTAAATCTT CTAACAGAAA AAGAAATAAA TACTGTTTTG 
CCGACACATT CTATTATCCC AAGAACCTTT GTCCTCAAGC CAGGAATGGT TTTATTTTTG 
GGTGGGATAG CCCGAATAGA TTTCCTACAG GGAAATCAGT CAGCCTGGTT CACAGTTGTG 
GCTTCCAACT TCCTTCCTGT TCATATTACT TCCTTGGACA AGGCAGATGC TCTGTATGAG 
AAACATGCAG GTCACGAGCT ACTGCTGGTT CCAATGGGTG GAAAAGAACG AATGGCCCAG 
TTTCCCCCTC TTGTTGCTGA AGACATTACA TTAAAGGGAG GTGGAAAATT TGAAGCAGTA 
GCTGATATCA AGTTCTCCTC TGCAGGTTGG GTTGCAGTAA CACCTTATTC TGAGGGCACG 
CTGCATCTCC GAGGTCACAC ACCTGAAGGG ACTGCTCTGA CTGTCCACCC CCCTGTCTTG 
CCATATATCG TTAATGTCAA AGGACAGCGC ATGAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCGT CCCTGGTGCA CAACCTGAAG AAGCACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062810.1
CDS	31..2112
Translation	MLPARLACGLLCGLRRGPAPAAACYGPARWLLEGKCEVPIRQRASSLGRRVPPSSTATEDYAEGPDTEERFLFPEYVPERTPEEQVRELQELRELQQLQQEKERERLQQREERLQQKLRAGFRTLPVPEFPDASVPPSGIYCSGCGAELHCQHPGLPGYLPEEKFRDAAQAEGGPARTVCQRCWLLVHHGRALRLQVSRDQYLELVSAALRRPGPALVLYMVNLLDLPDALLPDLPKLVGPKQLIVLGNKVDLLPQDAPGYLKRLRKRLWDDCIRAGLVVAPGHQGPQYPAGDEPLEEIKNQNPSSRSRTVVKDVRLISAKTGYGVEEMISALQRSWRYRGDVYLVGTTNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRQRRLQEDATKAEEDLSEEEQSQLNQLKKHGYIVGRVGRTFSYSREQDEVPFEFDADSLAFDMGSEPVVSVCKSTKQIELTPEDVKDAHWFYDTPGITKESCILNLLTEKEINTVLPTHSIIPRTFVLKPGMVLFLGGIARIDFLQGNQSAWFTVVASNFLPVHITSLDKADALYEKHAGHELLLVPMGGKERMAQFPPLVAEDITLKGGGKFEAVADIKFSSAGWVAVTPYSEGTLHLRGHTPEGTALTVHPPVLPYIVNVKGQRMKKSVAYKTKKPPSLVHNLKKHR

Target ORF information:

RefSeq Version	NM_019836.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide associated 1 (Noa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019836.3

ORF Insert Sequence:

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTAT TTTAAATCTT CTAACAGAAA AAGAAATAAA TACTGTTTTG 
CCGACACATT CTATTATCCC AAGAACCTTT GTCCTCAAGC CAGGAATGGT TTTATTTTTG 
GGTGGGATAG CCCGAATAGA TTTCCTACAG GGAAATCAGT CAGCCTGGTT CACAGTTGTG 
GCTTCCAACT TCCTTCCTGT TCATATTACT TCCTTGGACA AGGCAGATGC TCTGTATGAG 
AAACATGCAG GTCACGAGCT ACTGCTGGTT CCAATGGGTG GAAAAGAACG AATGGCCCAG 
TTTCCCCCTC TTGTTGCTGA AGACATTACA TTAAAGGGAG GTGGAAAATT TGAAGCAGTA 
GCTGATATCA AGTTCTCCTC TGCAGGTTGG GTTGCAGTAA CACCTTATTC TGAGGGCACG 
CTGCATCTCC GAGGTCACAC ACCTGAAGGG ACTGCTCTGA CTGTCCACCC CCCTGTCTTG 
CCATATATCG TTAATGTCAA AGGACAGCGC ATGAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCGT CCCTGGTGCA CAACCTGAAG AAGCACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Noa1 ( XM_006535153.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535216.1 Mus musculus nitric oxide associated 1 (Noa1), transcript variant X1, mRNA.

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CloneID	OMu35620
Clone ID Related Accession (Same CDS sequence)	XM_006535153.4 , XM_006535153.3
Accession Version	XM_006535153.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nitric oxide-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535153.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTGG AAATCAGTCA GCCTGGTTCA CAGTTGTGGC TTCCAACTTC 
CTTCCTGTTC ATATTACTTC CTTGGACAAG GCAGATGCTC TGTATGAGAA ACATGCAGGT 
CACGAGCTAC TGCTGGTTCC AATGGGTGGA AAAGAACGAA TGGCCCAGTT TCCCCCTCTT 
GTTGCTGAAG ACATTACATT AAAGGGAGGT GGAAAATTTG AAGCAGTAGC TGATATCAAG 
TTCTCCTCTG CAGGTTGGGT TGCAGTAACA CCTTATTCTG AGGGCACGCT GCATCTCCGA 
GGTCACACAC CTGAAGGGAC TGCTCTGACT GTCCACCCCC CTGTCTTGCC ATATATCGTT 
AATGTCAAAG GACAGCGCAT GAAGAAAAGT GTGGCCTATA AAACCAAGAA GCCTCCGTCC 
CTGGTGCACA ACCTGAAGAA GCACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535216.1
CDS	238..2187
Translation	MLPARLACGLLCGLRRGPAPAAACYGPARWLLEGKCEVPIRQRASSLGRRVPPSSTATEDYAEGPDTEERFLFPEYVPERTPEEQVRELQELRELQQLQQEKERERLQQREERLQQKLRAGFRTLPVPEFPDASVPPSGIYCSGCGAELHCQHPGLPGYLPEEKFRDAAQAEGGPARTVCQRCWLLVHHGRALRLQVSRDQYLELVSAALRRPGPALVLYMVNLLDLPDALLPDLPKLVGPKQLIVLGNKVDLLPQDAPGYLKRLRKRLWDDCIRAGLVVAPGHQGPQYPAGDEPLEEIKNQNPSSRSRTVVKDVRLISAKTGYGVEEMISALQRSWRYRGDVYLVGTTNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRQRRLQEDATKAEEDLSEEEQSQLNQLKKHGYIVGRVGRTFSYSREQDEVPFEFDADSLAFDMGSEPVVSVCKSTKQIELTPEDVKDAHWFYDTPGITKESCGNQSAWFTVVASNFLPVHITSLDKADALYEKHAGHELLLVPMGGKERMAQFPPLVAEDITLKGGGKFEAVADIKFSSAGWVAVTPYSEGTLHLRGHTPEGTALTVHPPVLPYIVNVKGQRMKKSVAYKTKKPPSLVHNLKKHR

Target ORF information:

RefSeq Version	XM_006535153.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535153.3

ORF Insert Sequence:

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTGG AAATCAGTCA GCCTGGTTCA CAGTTGTGGC TTCCAACTTC 
CTTCCTGTTC ATATTACTTC CTTGGACAAG GCAGATGCTC TGTATGAGAA ACATGCAGGT 
CACGAGCTAC TGCTGGTTCC AATGGGTGGA AAAGAACGAA TGGCCCAGTT TCCCCCTCTT 
GTTGCTGAAG ACATTACATT AAAGGGAGGT GGAAAATTTG AAGCAGTAGC TGATATCAAG 
TTCTCCTCTG CAGGTTGGGT TGCAGTAACA CCTTATTCTG AGGGCACGCT GCATCTCCGA 
GGTCACACAC CTGAAGGGAC TGCTCTGACT GTCCACCCCC CTGTCTTGCC ATATATCGTT 
AATGTCAAAG GACAGCGCAT GAAGAAAAGT GTGGCCTATA AAACCAAGAA GCCTCCGTCC 
CTGGTGCACA ACCTGAAGAA GCACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Noa1 ( XM_011249546.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247848.1 Mus musculus nitric oxide associated 1 (Noa1), transcript variant X2, mRNA.

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CloneID	OMu69650
Clone ID Related Accession (Same CDS sequence)	XM_011249546.3 , XM_011249546.2
Accession Version	XM_011249546.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nitric oxide-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTAT TTTAAATCTT CTAACAGAAA AAGAAATAAA TACTGTTTTG 
CCGACACATT CTATTATCCC AAGAACCTTT GTCCTCAAGC CAGGAATGGT TTTATTTTTG 
GGTGGGATAG CCCGAATAGA TTTCCTACAG GTGGGGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247848.1
CDS	238..1899
Translation	MLPARLACGLLCGLRRGPAPAAACYGPARWLLEGKCEVPIRQRASSLGRRVPPSSTATEDYAEGPDTEERFLFPEYVPERTPEEQVRELQELRELQQLQQEKERERLQQREERLQQKLRAGFRTLPVPEFPDASVPPSGIYCSGCGAELHCQHPGLPGYLPEEKFRDAAQAEGGPARTVCQRCWLLVHHGRALRLQVSRDQYLELVSAALRRPGPALVLYMVNLLDLPDALLPDLPKLVGPKQLIVLGNKVDLLPQDAPGYLKRLRKRLWDDCIRAGLVVAPGHQGPQYPAGDEPLEEIKNQNPSSRSRTVVKDVRLISAKTGYGVEEMISALQRSWRYRGDVYLVGTTNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRQRRLQEDATKAEEDLSEEEQSQLNQLKKHGYIVGRVGRTFSYSREQDEVPFEFDADSLAFDMGSEPVVSVCKSTKQIELTPEDVKDAHWFYDTPGITKESCILNLLTEKEINTVLPTHSIIPRTFVLKPGMVLFLGGIARIDFLQVGL

Target ORF information:

RefSeq Version	XM_011249546.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide associated 1 (Noa1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249546.2

ORF Insert Sequence:

ATGCTGCCCG CGCGCCTGGC TTGCGGGCTG CTCTGCGGGC TCCGGCGGGG CCCCGCGCCG 
GCCGCTGCGT GCTACGGCCC AGCACGGTGG CTTCTGGAGG GGAAGTGTGA GGTCCCCATC 
CGCCAGCGTG CATCATCCTT GGGCCGTCGG GTTCCCCCGA GCTCCACGGC GACCGAGGAC 
TATGCAGAAG GCCCGGACAC GGAGGAACGC TTTCTGTTCC CGGAGTACGT CCCGGAGCGG 
ACCCCGGAGG AACAAGTGCG GGAGTTGCAG GAGCTGCGGG AGTTGCAGCA GCTGCAGCAG 
GAGAAGGAAC GAGAGAGATT GCAGCAGCGG GAGGAGCGGC TTCAGCAGAA GCTGCGGGCG 
GGCTTCCGGA CGCTGCCTGT CCCGGAGTTT CCAGACGCCT CGGTGCCGCC CAGCGGCATC 
TACTGCTCGG GCTGCGGAGC CGAGCTGCAC TGCCAGCATC CCGGCCTGCC GGGCTACCTG 
CCGGAGGAGA AGTTCCGCGA CGCTGCCCAG GCGGAAGGCG GCCCGGCGCG GACCGTGTGC 
CAACGCTGCT GGCTGCTGGT GCACCACGGA CGAGCCCTGC GCCTGCAGGT AAGCCGCGAT 
CAGTACCTGG AGCTTGTGAG CGCAGCGCTG CGGCGTCCCG GGCCGGCTCT GGTGCTCTAT 
ATGGTGAATC TTCTAGACCT GCCGGACGCC CTGCTGCCCG ACCTGCCCAA GCTGGTGGGC 
CCCAAGCAAC TCATCGTGCT GGGGAACAAA GTGGACCTGC TGCCCCAGGA CGCTCCCGGC 
TACTTGAAGC GGCTGCGGAA GCGGCTGTGG GACGATTGCA TCCGCGCCGG GCTCGTGGTG 
GCTCCTGGTC ATCAAGGACC ACAGTACCCT GCCGGGGATG AGCCACTGGA GGAGATAAAG 
AATCAGAATC CGTCCTCCAG GTCCCGCACG GTAGTCAAGG ACGTGCGACT GATCAGCGCC 
AAGACGGGCT ATGGAGTTGA AGAAATGATC TCTGCTCTTC AGCGCTCCTG GCGCTACCGT 
GGTGATGTCT ACCTGGTGGG CACCACTAAT GCTGGCAAGT CCACTCTCTT TAACACACTC 
CTGGAGTCTG ATTACTGCAC CGCCAAGGGC TCCGAAGCCA TCGACCGGGC CACCATCTCC 
CCCTGGCCAG GCACCACATT AAACCTCCTG AAGTTTCCTA TTTGCAACCC GACTCCTTAC 
AGAATGTTCA AAAGACAAAG AAGGCTTCAA GAAGATGCGA CCAAAGCTGA GGAAGATCTT 
AGTGAGGAAG AACAAAGTCA GCTCAATCAA CTGAAAAAGC ACGGCTACAT AGTAGGAAGA 
GTTGGAAGAA CATTCTCGTA CTCCAGAGAA CAGGACGAAG TTCCCTTTGA GTTTGATGCC 
GACTCCCTGG CCTTTGACAT GGGAAGTGAA CCAGTTGTGT CTGTGTGCAA AAGCACCAAG 
CAAATAGAGC TAACCCCAGA GGACGTGAAA GACGCCCACT GGTTTTATGA CACCCCTGGA 
ATTACAAAAG AAAGCTGTAT TTTAAATCTT CTAACAGAAA AAGAAATAAA TACTGTTTTG 
CCGACACATT CTATTATCCC AAGAACCTTT GTCCTCAAGC CAGGAATGGT TTTATTTTTG 
GGTGGGATAG CCCGAATAGA TTTCCTACAG GTGGGGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NOA1, a novel ClpXP substrate, takes an unexpected nuclear detour prior to mitochondrial import.
PloS one9(7)e103141(2014)
Al-Furoukh N,Kardon JR,Kr?ger M,Szibor M,Baker TA,Braun T

Binding to G-quadruplex RNA activates the mitochondrial GTPase NOA1.
Biochimica et biophysica acta1833(12)2933-2942(2013 Dec)
Al-Furoukh N,Goffart S,Szibor M,Wanrooij S,Braun T

Nitric oxide-associated protein 1 (NOA1) is necessary for oxygen-dependent regulation of mitochondrial respiratory complexes.
The Journal of biological chemistry286(37)32086-93(2011 Sep)
Heidler J,Al-Furoukh N,Kukat C,Salwig I,Ingelmann ME,Seibel P,Kr?ger M,Holtz J,Wittig I,Braun T,Szibor M

NOA1 is an essential GTPase required for mitochondrial protein synthesis.
Molecular biology of the cell22(1)1-11(2011 Jan)
Kolanczyk M,Pech M,Zemojtel T,Yamamoto H,Mikula I,Calvaruso MA,van den Brand M,Richter R,Fischer B,Ritz A,Kossler N,Thurisch B,Spoerle R,Smeitink J,Kornak U,Chan D,Vingron M,Martasek P,Lightowlers RN,Nijtmans L,Schuelke M,Nierhaus KH,Mundlos S

mAtNOS1 induces apoptosis of human mammary adenocarcinoma cells.
Life sciences82(21-22)1077-82(2008 May)
Parihar A,Parihar MS,Chen Z,Ghafourifar P

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Noa1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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