Gde1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gde1
Entrez Gene ID	56209
Full Name	glycerophosphodiester phosphodiesterase 1
Synonyms	1200003M13Rik,Mir16,RGS16
General protein information	Preferred Names glycerophosphodiester phosphodiesterase 1 Names glycerophosphodiester phosphodiesterase 1 membrane interacting protein of RGS16
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_019580.4	NP_062526.1	glycerophosphodiester phosphodiesterase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GDE1	NP_057725.1
Canis lupus familiaris (dog)	GDE1	XP_536954.2
Gallus gallus (chicken)	GDE1	XP_414914.4
Xenopus tropicalis (tropical clawed frog)	gde1	NP_001017173.1
Pan troglodytes (chimpanzee)	GDE1	XP_510858.2
Danio rerio (zebrafish)	gde1	NP_956537.1
Drosophila melanogaster (fruit fly)	CG14883	NP_650547.1
Caenorhabditis elegans (roundworm)	T12B3.3	NP_501176.1
Caenorhabditis elegans (roundworm)	ZC155.4	NP_498106.1
	GDE1	XP_001084051.1
Bos taurus (cattle)	GDE1	NP_001029858.1
Mus musculus (house mouse)	Gde1	NP_062526.1
Rattus norvegicus (Norway rat)	Gde1	NP_116004.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001253	XP_321897.3
Caenorhabditis elegans (roundworm)	T22G5.1	NP_506439.1
Caenorhabditis elegans (roundworm)	T09B9.3	NP_509638.2

Gene Ontology
Bibliography

Related articles in PubMed

The genetic architecture of NAFLD among inbred strains of mice.
Hui ST, Parks BW, Org E, Norheim F, Che N, Pan C, Castellani LW, Charugundla S, Dirks DL, Psychogios N, Neuhaus I, Gerszten RE, Kirchgessner T, Gargalovic PS, Lusis AJ
eLife4e05607(2015 Jun)

Glycerophosphodiesterase GDE4 as a novel lysophospholipase D: a possible involvement in bioactive N-acylethanolamine biosynthesis.
Tsuboi K, Okamoto Y, Rahman IA, Uyama T, Inoue T, Tokumura A, Ueda N
Biochimica et biophysica acta1851(5)537-48(2015 May)

Metabolism of endocannabinoids and related N-acylethanolamines: canonical and alternative pathways.
Ueda N, Tsuboi K, Uyama T
The FEBS journal280(9)1874-94(2013 May)

Inhibition of miR-15 protects against cardiac ischemic injury.
Hullinger TG, Montgomery RL, Seto AG, Dickinson BA, Semus HM, Lynch JM, Dalby CM, Robinson K, Stack C, Latimer PA, Hare JM, Olson EN, van Rooij E
Circulation research110(1)71-81(2012 Jan)

The glycerophospho metabolome and its influence on amino acid homeostasis revealed by brain metabolomics of GDE1(-/-) mice.
Kopp F, Komatsu T, Nomura DK, Trauger SA, Thomas JR, Siuzdak G, Simon GM, Cravatt BF
Chemistry & biology17(8)831-40(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors confirmed the role of Gde1 in NAFLD by in vivo hepatic over-expression and shRNA knockdown studies.
Title: The genetic architecture of NAFLD among inbred strains of mice.

Characterization of mice lacking N-acyl ethanolamine biosynthetic enzymes.
Title: Characterization of mice lacking candidate N-acyl ethanolamine biosynthetic enzymes provides evidence for multiple pathways that contribute to endocannabinoid production in vivo.

findings designate GroPSer as a previously unappreciated reservoir for free serine in the nervous system and suggest that GDE1, through recycling serine from GroPSer, may impact D-serine-dependent neural signaling processes in vivo
Title: The glycerophospho metabolome and its influence on amino acid homeostasis revealed by brain metabolomics of GDE1(-/-) mice.

a multistep pathway for the production of anandamide in the nervous system by the sequential actions of Abh4 and GDE1.
Title: Anandamide biosynthesis catalyzed by the phosphodiesterase GDE1 and detection of glycerophospho-N-acyl ethanolamine precursors in mouse brain.

regulation by by stimulation of G protein-coupled receptors
Title: GDE1/MIR16 is a glycerophosphoinositol phosphodiesterase regulated by stimulation of G protein-coupled receptors.

The following Gde1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gde1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11206	NM_019580.4 Latest version!	Mus musculus glycerophosphodiester phosphodiesterase 1 (Gde1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gde1 ( NM_019580.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062526.1 Mus musculus glycerophosphodiester phosphodiesterase 1 (Gde1), mRNA.

Price & Availability↑

CloneID	OMu11206
Clone ID Related Accession (Same CDS sequence)	NM_019580.4
Accession Version	NM_019580.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	glycerophosphodiester phosphodiesterase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK005361.1. On Apr 5, 2007 this sequence version replaced NM_019580.3. ##Evidence-Data-START## Transcript exon combination :: AK005361.1, AK150807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCTGT GGGAGGACCA GGGCGGCCTC CTGGGCCCCT TCTCCTTCGT GCTGGTGCTG 
CTGCTGGTGG TGACACGCAG CCCCTTCAAC GCCTGCGTGC TCACCGGCAG CCTCTACATC 
CTGCTGCGGT TCTTCAGCTT CGAGCCGGTG CCGTCCCGCC GGGCCCTGCA GGTGCTCAAG 
CCCCGTGACC GCGTGTCCGC CATCGCCCAT CGCGGCGGCA GCCACGACGC GCCCGAGAAC 
ACGCTGGCGG CCATCCGGCA GGCAGCTAAG AATGGAGCAA CAGGTGTGGA GCTGGACATC 
GAGTTTACTT CTGATGGAGT TCCTGTCTTG ATGCACGATA ACACAGTAGA TAGGACAACA 
GACGGGTCTG GCCGACTATG TGATCTGACA TTTGAACAAG TTAGGAAACT TAACCCTGCA 
GCAAATCACA GACTCAGAAA CGAGTTCCCT GATGAAAGGA TCCCTACCCT GAAGGAAGCT 
GTCACAGAGT GCCTCCGCCA TAACCTCACC ATCTTCTTTG ATGTCAAAGG CCATGCAGAT 
ATGGCTTCTG CTGCTCTAAA GAATATATAC ACGGAATTTC CACAGCTGTA CAATAACAGT 
ATGGTCTGCT CATTCTTGCC GGAAGTCATC TATAAAATGA GACAAACGGA TCAGAAGGTA 
ATCACAGCCC TAACTCACAG GCCTTGGAGC CTGAGCCACA CTGGAGATGG GAAGCCTCGC 
TACAGCGTCT TTTGGAAGCA GTCTGTGTTT GTGGTGTTGG ACATCTTGCT GGACTGGAGC 
ATGCACAATG TCTTGTGGTA CCTGTGTGGG ATTTCAGCCT TCCTCATGCA GAAGGATTTT 
GTCTCCCCGG ACTATTTGAA GAAGTGGTCA GCTAAAGGAA TCCAGGTTGT GAGTTGGACT 
GTCAATACCT TTGATGAAAA GAATTACTAC GAGTCCCACC TTGGGTCCAG CTACATCACT 
GACAGCATGC TAGAAGACTG TGCACCTCAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062526.1
CDS	89..1084
Translation	MWLWEDQGGLLGPFSFVLVLLLVVTRSPFNACVLTGSLYILLRFFSFEPVPSRRALQVLKPRDRVSAIAHRGGSHDAPENTLAAIRQAAKNGATGVELDIEFTSDGVPVLMHDNTVDRTTDGSGRLCDLTFEQVRKLNPAANHRLRNEFPDERIPTLKEAVTECLRHNLTIFFDVKGHADMASAALKNIYTEFPQLYNNSMVCSFLPEVIYKMRQTDQKVITALTHRPWSLSHTGDGKPRYSVFWKQSVFVVLDILLDWSMHNVLWYLCGISAFLMQKDFVSPDYLKKWSAKGIQVVSWTVNTFDEKNYYESHLGSSYITDSMLEDCAPHF

Target ORF information:

RefSeq Version	NM_019580.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glycerophosphodiester phosphodiesterase 1 (Gde1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019580.4

ORF Insert Sequence:

ATGTGGCTGT GGGAGGACCA GGGCGGCCTC CTGGGCCCCT TCTCCTTCGT GCTGGTGCTG 
CTGCTGGTGG TGACACGCAG CCCCTTCAAC GCCTGCGTGC TCACCGGCAG CCTCTACATC 
CTGCTGCGGT TCTTCAGCTT CGAGCCGGTG CCGTCCCGCC GGGCCCTGCA GGTGCTCAAG 
CCCCGTGACC GCGTGTCCGC CATCGCCCAT CGCGGCGGCA GCCACGACGC GCCCGAGAAC 
ACGCTGGCGG CCATCCGGCA GGCAGCTAAG AATGGAGCAA CAGGTGTGGA GCTGGACATC 
GAGTTTACTT CTGATGGAGT TCCTGTCTTG ATGCACGATA ACACAGTAGA TAGGACAACA 
GACGGGTCTG GCCGACTATG TGATCTGACA TTTGAACAAG TTAGGAAACT TAACCCTGCA 
GCAAATCACA GACTCAGAAA CGAGTTCCCT GATGAAAGGA TCCCTACCCT GAAGGAAGCT 
GTCACAGAGT GCCTCCGCCA TAACCTCACC ATCTTCTTTG ATGTCAAAGG CCATGCAGAT 
ATGGCTTCTG CTGCTCTAAA GAATATATAC ACGGAATTTC CACAGCTGTA CAATAACAGT 
ATGGTCTGCT CATTCTTGCC GGAAGTCATC TATAAAATGA GACAAACGGA TCAGAAGGTA 
ATCACAGCCC TAACTCACAG GCCTTGGAGC CTGAGCCACA CTGGAGATGG GAAGCCTCGC 
TACAGCGTCT TTTGGAAGCA GTCTGTGTTT GTGGTGTTGG ACATCTTGCT GGACTGGAGC 
ATGCACAATG TCTTGTGGTA CCTGTGTGGG ATTTCAGCCT TCCTCATGCA GAAGGATTTT 
GTCTCCCCGG ACTATTTGAA GAAGTGGTCA GCTAAAGGAA TCCAGGTTGT GAGTTGGACT 
GTCAATACCT TTGATGAAAA GAATTACTAC GAGTCCCACC TTGGGTCCAG CTACATCACT 
GACAGCATGC TAGAAGACTG TGCACCTCAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genetic architecture of NAFLD among inbred strains of mice.
eLife4e05607(2015 Jun)
Hui ST,Parks BW,Org E,Norheim F,Che N,Pan C,Castellani LW,Charugundla S,Dirks DL,Psychogios N,Neuhaus I,Gerszten RE,Kirchgessner T,Gargalovic PS,Lusis AJ

Glycerophosphodiesterase GDE4 as a novel lysophospholipase D: a possible involvement in bioactive N-acylethanolamine biosynthesis.
Biochimica et biophysica acta1851(5)537-48(2015 May)
Tsuboi K,Okamoto Y,Rahman IA,Uyama T,Inoue T,Tokumura A,Ueda N

Metabolism of endocannabinoids and related N-acylethanolamines: canonical and alternative pathways.
The FEBS journal280(9)1874-94(2013 May)
Ueda N,Tsuboi K,Uyama T

Inhibition of miR-15 protects against cardiac ischemic injury.
Circulation research110(1)71-81(2012 Jan)
Hullinger TG,Montgomery RL,Seto AG,Dickinson BA,Semus HM,Lynch JM,Dalby CM,Robinson K,Stack C,Latimer PA,Hare JM,Olson EN,van Rooij E

The glycerophospho metabolome and its influence on amino acid homeostasis revealed by brain metabolomics of GDE1(-/-) mice.
Chemistry & biology17(8)831-40(2010 Aug)
Kopp F,Komatsu T,Nomura DK,Trauger SA,Thomas JR,Siuzdak G,Simon GM,Cravatt BF