Ramp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ramp2
Entrez Gene ID	54409
Full Name	receptor (calcitonin) activity modifying protein 2
General protein information	Preferred Names receptor (calcitonin) activity modifying protein 2 Names receptor activity-modifying protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 D\|11 64.47 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_019444.2	NP_062317.1	receptor activity-modifying protein 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RAMP2	XP_537636.1
	RAMP2	XP_001096145.2
Homo sapiens (human)	RAMP2	NP_005845.2
Gallus gallus (chicken)	RAMP2	NP_001099080.1
Pan troglodytes (chimpanzee)	RAMP2	XP_001160651.1
Bos taurus (cattle)	RAMP2	NP_001092330.1
Mus musculus (house mouse)	Ramp2	NP_062317.1
Rattus norvegicus (Norway rat)	Ramp2	NP_113834.1

Gene Ontology
Bibliography

Related articles in PubMed

Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.
Xian X, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Tanaka M, Koyama T, Kawate H, Yang L, Liu T, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T
Endocrinology158(5)1359-1372(2017 May)

Cardiovascular effects of exogenous adrenomedullin and CGRP in Ramp and Calcrl deficient mice.
Pawlak JB, Wetzel-Strong SE, Dunn MK, Caron KM
Peptides881-7(2017 Feb)

Loss of receptor activity-modifying protein 2 in mice causes placental dysfunction and alters PTH1R regulation.
Kadmiel M, Matson BC, Espenschied ST, Lenhart PM, Caron KM
PloS one12(7)e0181597(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The AM-RAMP2 system exerts crucial vasoprotective effects after vascular injury and could be a therapeutic target for the treatment of vascular diseases.
Title: Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.

the cardiovascular response of Ramp1(-/-), Ramp2(+/-), Ramp3(-/-), Ramp1(-/-)/Ramp3(-/-) double-knockout (dKO), and Calcrl(+/-) mice to AM and CGRP were compared to wildtype mice.These results suggest that the hypotensive effect of AM is primarily mediated through the CLR/RAMP1 heterodimer, but that AM signaling via CLR/RAMP2 and CLR/RAMP3 also contributes to some hypotensive action.
Title: Cardiovascular effects of exogenous adrenomedullin and CGRP in Ramp and Calcrl deficient mice.

Results provide in vivo evidence of a role for RAMP2 in placental development distinct from the RAMP2-calcitonin receptor-like receptor/adrenomedullin signaling paradigm; decreases Pthr1 expression and causes a blunted response to systemic parathyroid hormone; and identify additional pathways underlying the endocrine and fertility defects of the previously characterized Ramp2 heterozygous adult females.
Title: Loss of receptor activity-modifying protein 2 in mice causes placental dysfunction and alters PTH1R regulation.

the Adrenomedullin-RAMP2 system regulates vascular integrity, whereas RAMP2 deletion promotes vascular permeability
Title: The endothelial adrenomedullin-RAMP2 system regulates vascular integrity and suppresses tumour metastasis.

RAMP2 is essential for early vascular development and knockout-mice die in utero.
Title: Functional differentiation of RAMP2 and RAMP3 in their regulation of the vascular system.

The following Ramp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ramp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10937	NM_019444.2 Latest version!	Mus musculus receptor (calcitonin) activity modifying protein 2 (Ramp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ramp2 ( NM_019444.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062317.1 Mus musculus receptor (calcitonin) activity modifying protein 2 (Ramp2), mRNA.

Price & Availability↑

CloneID	OMu10937
Clone ID Related Accession (Same CDS sequence)	NM_019444.2
Accession Version	NM_019444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Mus musculus(house mouse)
Product	receptor activity-modifying protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY312723.1, AK003810.1 and BC051201.1. On Jul 28, 2005 this sequence version replaced NM_019444.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF146523.1, BY704069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCGC TCCGGGTAGA GCGCGCCCCG GGTGGATCTC GGCTTGGTGT GACCCGCGCC 
CAGCGGCCGA CAGCGTTGTG CCTCCCTCCG CTGTTGCTGC TGCTGCTGCT GCTGCTGGGC 
GCTGTCTCAG CCTCTCCGGA GTCCCTGAAC CAATCTCTTC CGGAGTCCCA GAATCAATCT 
CATCCCACTG AGGACAGCCT TGTGTCAAAA GGGAAGATGG AAGACTACGA AACACATGTC 
CTACCTTGCT GGTATGAGTA CAAGAGTTGC ATGGACTCTG TCAAGGACTG GTGCAACTGG 
ACTTTGATTA GCAGGCATTA CAGCGACCTG CAGAACTGCT TGGAATACAA TGCAGACAAG 
TTTGGGCTGG GCTTCCCAAA TCCCTTGGCA GAAAACATCA TCCTTGAGGC TCACCTGATA 
CACTTTGCGA ACTGCTCCCT GGTGCAGCCC ACCTTCTCTG ATCCCCCAGA GGATGTGCTC 
CTGGCCATGA TCATAGCCCC CATCTGCCTC ATCCCGTTCC TTGTTACTCT TGTGGTGTGG 
AGGAGTAAAG ACAGCGATGC CCAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062317.1
CDS	119..688
Translation	MAPLRVERAPGGSRLGVTRAQRPTALCLPPLLLLLLLLLGAVSASPESLNQSLPESQNQSHPTEDSLVSKGKMEDYETHVLPCWYEYKSCMDSVKDWCNWTLISRHYSDLQNCLEYNADKFGLGFPNPLAENIILEAHLIHFANCSLVQPTFSDPPEDVLLAMIIAPICLIPFLVTLVVWRSKDSDAQA

Target ORF information:

RefSeq Version	NM_019444.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus receptor (calcitonin) activity modifying protein 2 (Ramp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019444.2

ORF Insert Sequence:

ATGGCCCCGC TCCGGGTAGA GCGCGCCCCG GGTGGATCTC GGCTTGGTGT GACCCGCGCC 
CAGCGGCCGA CAGCGTTGTG CCTCCCTCCG CTGTTGCTGC TGCTGCTGCT GCTGCTGGGC 
GCTGTCTCAG CCTCTCCGGA GTCCCTGAAC CAATCTCTTC CGGAGTCCCA GAATCAATCT 
CATCCCACTG AGGACAGCCT TGTGTCAAAA GGGAAGATGG AAGACTACGA AACACATGTC 
CTACCTTGCT GGTATGAGTA CAAGAGTTGC ATGGACTCTG TCAAGGACTG GTGCAACTGG 
ACTTTGATTA GCAGGCATTA CAGCGACCTG CAGAACTGCT TGGAATACAA TGCAGACAAG 
TTTGGGCTGG GCTTCCCAAA TCCCTTGGCA GAAAACATCA TCCTTGAGGC TCACCTGATA 
CACTTTGCGA ACTGCTCCCT GGTGCAGCCC ACCTTCTCTG ATCCCCCAGA GGATGTGCTC 
CTGGCCATGA TCATAGCCCC CATCTGCCTC ATCCCGTTCC TTGTTACTCT TGTGGTGTGG 
AGGAGTAAAG ACAGCGATGC CCAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.
Endocrinology158(5)1359-1372(2017 May)
Xian X,Sakurai T,Kamiyoshi A,Ichikawa-Shindo Y,Tanaka M,Koyama T,Kawate H,Yang L,Liu T,Imai A,Zhai L,Hirabayashi K,Dai K,Tanimura K,Liu T,Cui N,Igarashi K,Yamauchi A,Shindo T

Cardiovascular effects of exogenous adrenomedullin and CGRP in Ramp and Calcrl deficient mice.
Peptides881-7(2017 Feb)
Pawlak JB,Wetzel-Strong SE,Dunn MK,Caron KM

Loss of receptor activity-modifying protein 2 in mice causes placental dysfunction and alters PTH1R regulation.
PloS one12(7)e0181597(2017)
Kadmiel M,Matson BC,Espenschied ST,Lenhart PM,Caron KM