Anapc4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Anapc4
Entrez Gene ID	52206
Full Name	anaphase promoting complex subunit 4
Synonyms	2610306D21Rik,APC4,D5Ertd249e
General protein information	Preferred Names anaphase promoting complex subunit 4 Names anaphase-promoting complex subunit 4 cyclosome subunit 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 C1\|5 28.81 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_024213.2	NP_077175.1	anaphase-promoting complex subunit 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ANAPC4	XP_001165393.1
Homo sapiens (human)	ANAPC4	NP_001273685.1
Drosophila melanogaster (fruit fly)	APC4	NP_572522.2
	ANAPC4	XP_001082716.2
Canis lupus familiaris (dog)	ANAPC4	XP_536270.3
Bos taurus (cattle)	ANAPC4	NP_001179307.1
Mus musculus (house mouse)	Anapc4	NP_077175.1
Gallus gallus (chicken)	ANAPC4	XP_004936201.1
Danio rerio (zebrafish)	anapc4	NP_001008589.1
Rattus norvegicus (Norway rat)	Anapc4	NP_001100690.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002345	XP_312619.4
Xenopus tropicalis (tropical clawed frog)	anapc4	NP_001135602.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Extensive molecular differences between anterior- and posterior-half-sclerotomes underlie somite polarity and spinal nerve segmentation.
Hughes DS, Keynes RJ, Tannahill D
BMC developmental biology930(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Anapc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Anapc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02342	NM_024213.2 Latest version!	Mus musculus anaphase promoting complex subunit 4 (Anapc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Anapc4 ( NM_024213.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077175.1 Mus musculus anaphase promoting complex subunit 4 (Anapc4), mRNA.

Price & Availability↑

CloneID	OMu02342
Clone ID Related Accession (Same CDS sequence)	NM_024213.2
Accession Version	NM_024213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	anaphase-promoting complex subunit 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC102766.5. On Mar 21, 2007 this sequence version replaced NM_024213.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC024870.1, AK148625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCGCT TTCCGACCTG TTTCCCATCC TTTCGGGTGG TGGGAGAGAA GCAGCTGCCA 
CAGGAGATTA TTTTCTTGGC CTGGTCCCCC AAGCGAGACC TCATTGCTTT GGCCAACACT 
ACAGGCGAGG TTTTGCTGCA TCGCCTTGCA AGTTTTCATC GAGTTTGGAG TTTCCCACCA 
AATGAAAGTA CAGGAAAAGA AGTGACCTGC TTGGCCTGGA GACCAGACGG CAAACTTTTG 
GCCTTTGCTC TTGCGGATAC CAAGAAAATT ATTTTGTGTG ATGTAGAAAA GCCTGAAAGC 
TTACACTCCT TCTCTGTGGA GGCTCCGGTC TCTTGTATGC ATTGGACAGA AGTGACTGTG 
GAAAGCAGTG TTTTAACATC GTTTTATAAT GCTGAGGATG AGTCCAATCT TCTCTTGCCT 
AAGCTGCCCA CATTGCCAAA AAATTACAAC AGCACCTCAA AAATATTTAG TGAAGAAAAT 
TCTGATGAAA TTATCAAGCT CTTGGGAGAT GTCAGGCTGA ACATCCTTGT CCTTGGAGGA 
AGCTCTGGAT TTATTGAGCT TTATGCTTAT GGGATGTTCA AAATTGCCCG AGTAACAGGG 
ATTGCCGGAA CTTGTATTGC TCTGTGTTTA TCAAGTGATT TGAAGTCATT ATCAGTGGTC 
ACAGAGGTTT CCAGCGGTGG CGAATCAGAA GTTTCATATT TTCAGCTTGA AACCAATCTG 
CTGTATTCCT TCTTACCTGA AGTAACTCGT ATGGCCAGGA AGTTTACTCA CATTTCAGCT 
CTCTTACAGT ATATAAATTT GTCACTGACA TGTATGTGTG AAGCATGGGA AGAAATTCTG 
ATGCAGATGG ACTCCCGTCT TACCAAGTTT GTACAGGAAA AACCTACAAC CACGTCAGTG 
CAGGATGAGT TCATGCACTT GCTGCTGTGG GGGAAAGCCA GTGCTGAACT TCAGACTCTC 
CTGATGAACC AGTTAACAGT GAAGGGCTTA AAAAAGCTTG GCCAGTCTAT AGAGTCATCA 
TATTCCAGTA TACAAAAACT GGTTATAAGT CACTTACAGA GTGGCTCTGA GTCTTTACTG 
TATCATTTGA GTGAACTTAA AGGAATGGCT TCATGGAAAC AAAAATACGA ACCTCTAGGA 
CTGGATGCTG CAGGCATTGA AGATGCTATT ACTGCTGTGG GTTCTTTCAT CCTCAAAGCA 
AACGAACTTC TCCAAGTTAT AGACAGTAGT ATGAAAAACT TCAAGGCATT TTTTCGGTGG 
CTGTATGTAG CAATGCTGAG AATGACAGAG GACCATGTGC TTCCTGAGCT GAACAAGATG 
ACTCAGAAGG ATATCACATT TGTTGCTGAA TTCCTCACTG AACATTTCAA TGAGGCTCCA 
GACCTTTATA ATAGGAAAGG AAAATACTTC AATGTTGAAA GAGTTGGTCA GTATTTGAAA 
GATGAAGACG ACGACCTTGT GTCACCCCCT AACACAGAAG GAAACCAGTG GTATGACTTC 
CTCCAAAACA GCACCCACCT GAAAGAAAGT CCTTTGCTGT TTCCTTATTA CCCTCGAAAA 
TCATTGCATT TTGTGAAAAG GCGGATGGAG AACGTTATTG ATCAGTGTTT GCAAAAGCCA 
GCAGATGTAA TTGGAAGATC GATGAATCAA GCAATTTGCA TTCCATTATA TAAAGATGCT 
AGGAGTATGG ACTGTGCACG AAGATTGTTA AAATTCCCAT TTCTGTGGAA TAATAAAACT 
TCGAATCTTC ATTATCTTCT TTTTACTATT TTAGAAGATT CGGTTTATAA AATGTGCATC 
TTAAGGAGAC ATACTGATAT TTCCCAATCT GTAAGTAATG GACTAATTGG TATTAAATTT 
GGGAGCTTCA CATCTGCCTC AGCTGACAAG GTCAGAAGAA GCTCTTACAG TTGTCTAGAC 
GCCCAGTTCT ATGATGATGA GACTGTCACG GTCATCCTGA AGGACTCCAT GGGACGTGAA 
GGGAGAGACA GGATTTTGGT TCAGTTGTCA CTGTCCTTGG TGTATAACAG TGAAGATTCT 
GACGAATATG AGTTCACTGG GAGTTACTCG ACAAGGCTTG ACGAGCAGGG CAGCATTATC 
CCCACACGCA CCATGCACTT TGAGAAACAC TGGAGGCTGC TAGAAAGCAT GAGAGCGCAG 
TATGTTGCTG GGAACGGCCT TCGAAAAGTG TCCTGTGTGT TAAGTTCAAA CCTCCGGCAT 
GTGAGGGTGT TTGAAATGGA CATCGATGAT GAGTGGGAGA TTGACGAGTC TTCAGACGAT 
GAGGAGGAGG CTGGCGGGAA GCCTGTGAAG ATCAAGGAGG AAGTGTTGTC AGAGTCAGAG 
ACAGAGGCTC ACCAAGACGC TGCTGCCCTA GACCCGGACG TAGTCATCAA AGTGGAGAAA 
CTTGACCCTG AGTTGGACTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077175.1
CDS	130..2553
Translation	MLRFPTCFPSFRVVGEKQLPQEIIFLAWSPKRDLIALANTTGEVLLHRLASFHRVWSFPPNESTGKEVTCLAWRPDGKLLAFALADTKKIILCDVEKPESLHSFSVEAPVSCMHWTEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYNSTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCIALCLSSDLKSLSVVTEVSSGGESEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKPTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEDAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSTHLKESPLLFPYYPRKSLHFVKRRMENVIDQCLQKPADVIGRSMNQAICIPLYKDARSMDCARRLLKFPFLWNNKTSNLHYLLFTILEDSVYKMCILRRHTDISQSVSNGLIGIKFGSFTSASADKVRRSSYSCLDAQFYDDETVTVILKDSMGREGRDRILVQLSLSLVYNSEDSDEYEFTGSYSTRLDEQGSIIPTRTMHFEKHWRLLESMRAQYVAGNGLRKVSCVLSSNLRHVRVFEMDIDDEWEIDESSDDEEEAGGKPVKIKEEVLSESETEAHQDAAALDPDVVIKVEKLDPELDS

Target ORF information:

RefSeq Version	NM_024213.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anaphase promoting complex subunit 4 (Anapc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024213.2

ORF Insert Sequence:

ATGCTGCGCT TTCCGACCTG TTTCCCATCC TTTCGGGTGG TGGGAGAGAA GCAGCTGCCA 
CAGGAGATTA TTTTCTTGGC CTGGTCCCCC AAGCGAGACC TCATTGCTTT GGCCAACACT 
ACAGGCGAGG TTTTGCTGCA TCGCCTTGCA AGTTTTCATC GAGTTTGGAG TTTCCCACCA 
AATGAAAGTA CAGGAAAAGA AGTGACCTGC TTGGCCTGGA GACCAGACGG CAAACTTTTG 
GCCTTTGCTC TTGCGGATAC CAAGAAAATT ATTTTGTGTG ATGTAGAAAA GCCTGAAAGC 
TTACACTCCT TCTCTGTGGA GGCTCCGGTC TCTTGTATGC ATTGGACAGA AGTGACTGTG 
GAAAGCAGTG TTTTAACATC GTTTTATAAT GCTGAGGATG AGTCCAATCT TCTCTTGCCT 
AAGCTGCCCA CATTGCCAAA AAATTACAAC AGCACCTCAA AAATATTTAG TGAAGAAAAT 
TCTGATGAAA TTATCAAGCT CTTGGGAGAT GTCAGGCTGA ACATCCTTGT CCTTGGAGGA 
AGCTCTGGAT TTATTGAGCT TTATGCTTAT GGGATGTTCA AAATTGCCCG AGTAACAGGG 
ATTGCCGGAA CTTGTATTGC TCTGTGTTTA TCAAGTGATT TGAAGTCATT ATCAGTGGTC 
ACAGAGGTTT CCAGCGGTGG CGAATCAGAA GTTTCATATT TTCAGCTTGA AACCAATCTG 
CTGTATTCCT TCTTACCTGA AGTAACTCGT ATGGCCAGGA AGTTTACTCA CATTTCAGCT 
CTCTTACAGT ATATAAATTT GTCACTGACA TGTATGTGTG AAGCATGGGA AGAAATTCTG 
ATGCAGATGG ACTCCCGTCT TACCAAGTTT GTACAGGAAA AACCTACAAC CACGTCAGTG 
CAGGATGAGT TCATGCACTT GCTGCTGTGG GGGAAAGCCA GTGCTGAACT TCAGACTCTC 
CTGATGAACC AGTTAACAGT GAAGGGCTTA AAAAAGCTTG GCCAGTCTAT AGAGTCATCA 
TATTCCAGTA TACAAAAACT GGTTATAAGT CACTTACAGA GTGGCTCTGA GTCTTTACTG 
TATCATTTGA GTGAACTTAA AGGAATGGCT TCATGGAAAC AAAAATACGA ACCTCTAGGA 
CTGGATGCTG CAGGCATTGA AGATGCTATT ACTGCTGTGG GTTCTTTCAT CCTCAAAGCA 
AACGAACTTC TCCAAGTTAT AGACAGTAGT ATGAAAAACT TCAAGGCATT TTTTCGGTGG 
CTGTATGTAG CAATGCTGAG AATGACAGAG GACCATGTGC TTCCTGAGCT GAACAAGATG 
ACTCAGAAGG ATATCACATT TGTTGCTGAA TTCCTCACTG AACATTTCAA TGAGGCTCCA 
GACCTTTATA ATAGGAAAGG AAAATACTTC AATGTTGAAA GAGTTGGTCA GTATTTGAAA 
GATGAAGACG ACGACCTTGT GTCACCCCCT AACACAGAAG GAAACCAGTG GTATGACTTC 
CTCCAAAACA GCACCCACCT GAAAGAAAGT CCTTTGCTGT TTCCTTATTA CCCTCGAAAA 
TCATTGCATT TTGTGAAAAG GCGGATGGAG AACGTTATTG ATCAGTGTTT GCAAAAGCCA 
GCAGATGTAA TTGGAAGATC GATGAATCAA GCAATTTGCA TTCCATTATA TAAAGATGCT 
AGGAGTATGG ACTGTGCACG AAGATTGTTA AAATTCCCAT TTCTGTGGAA TAATAAAACT 
TCGAATCTTC ATTATCTTCT TTTTACTATT TTAGAAGATT CGGTTTATAA AATGTGCATC 
TTAAGGAGAC ATACTGATAT TTCCCAATCT GTAAGTAATG GACTAATTGG TATTAAATTT 
GGGAGCTTCA CATCTGCCTC AGCTGACAAG GTCAGAAGAA GCTCTTACAG TTGTCTAGAC 
GCCCAGTTCT ATGATGATGA GACTGTCACG GTCATCCTGA AGGACTCCAT GGGACGTGAA 
GGGAGAGACA GGATTTTGGT TCAGTTGTCA CTGTCCTTGG TGTATAACAG TGAAGATTCT 
GACGAATATG AGTTCACTGG GAGTTACTCG ACAAGGCTTG ACGAGCAGGG CAGCATTATC 
CCCACACGCA CCATGCACTT TGAGAAACAC TGGAGGCTGC TAGAAAGCAT GAGAGCGCAG 
TATGTTGCTG GGAACGGCCT TCGAAAAGTG TCCTGTGTGT TAAGTTCAAA CCTCCGGCAT 
GTGAGGGTGT TTGAAATGGA CATCGATGAT GAGTGGGAGA TTGACGAGTC TTCAGACGAT 
GAGGAGGAGG CTGGCGGGAA GCCTGTGAAG ATCAAGGAGG AAGTGTTGTC AGAGTCAGAG 
ACAGAGGCTC ACCAAGACGC TGCTGCCCTA GACCCGGACG TAGTCATCAA AGTGGAGAAA 
CTTGACCCTG AGTTGGACTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Extensive molecular differences between anterior- and posterior-half-sclerotomes underlie somite polarity and spinal nerve segmentation.
BMC developmental biology930(2009 May)
Hughes DS,Keynes RJ,Tannahill D