Figla cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Figla
Entrez Gene ID	26910
Full Name	folliculogenesis specific basic helix-loop-helix
Synonyms	FIGalpha,bHLHc8
General protein information	Preferred Names folliculogenesis specific basic helix-loop-helix Names factor in the germline alpha FIG alpha transcription factor FIGa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321595.1	XP_017177084.1	factor in the germline alpha isoform X1
NM_012013.2	NP_036143.1	factor in the germline alpha

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FIGLA	XP_001138053.1
Bos taurus (cattle)	FIGLA	XP_002691278.2
	LOC710606	XP_001099273.1
Mus musculus (house mouse)	Figla	NP_036143.1
Homo sapiens (human)	FIGLA	NP_001004311.2
Canis lupus familiaris (dog)	FIGLA	XP_005626316.1
Rattus norvegicus (Norway rat)	Figla	XP_575589.1

Gene Ontology
Bibliography

Related articles in PubMed

Associations among Sebox and other MEGs and its effects on early embryogenesis.
Park MW, Kim KH, Kim EY, Lee SY, Ko JJ, Lee KA
PloS one10(2)e0115050(2015)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Simultaneous gene deletion of gata4 and gata6 leads to early disruption of follicular development and germ cell loss in the murine ovary.
Padua MB, Fox SC, Jiang T, Morse DA, Tevosian SG
Biology of reproduction91(1)24(2014 Jul)

Notch signaling regulates ovarian follicle formation and coordinates follicular growth.
Vanorny DA, Prasasya RD, Chalpe AJ, Kilen SM, Mayo KE
Molecular endocrinology (Baltimore, Md.)28(4)499-511(2014 Apr)

Figla-Cre transgenic mice expressing myristoylated EGFP in germ cells provide a model for investigating perinatal oocyte dynamics.
Lin RS, Jimenez-Movilla M, Dean J
PloS one9(1)e84477(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GFP positive cells showed the expression of germ cell markers Vasa, meiotic specific gene Stra8, Scp3, oocyte markers Gdf9, Zp3 and Figla, indicating that this method could be used for the purification and selection of female germ cells
Title: Characterization of female germ-like cells derived from mouse embryonic stem cells through expression of GFP under the control of Figla promoter.

Data suggest that a dynamic DNA CpG methylation in Sohlh2 and Figlalpha promoters, but not in the intron and exon sequences, is linked to regulation of gene expression, even though CpG islands are also present in their introns or exons.
Title: DNA methylation of germ-cell-specific basic helix-loop-helix (HLH) transcription factors, Sohlh2 and Figl? during gametogenesis.

physiological expression of Figla plays a critical dual role in activation of oocyte-associated genes and repression of sperm-associated genes during normal postnatal oogenesis
Title: FIGLA, a basic helix-loop-helix transcription factor, balances sexually dimorphic gene expression in postnatal oocytes.

FIGLA is a central regulator of oocyte-specific genes that play roles in folliculogenesis, fertilization and early development.
Title: Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor.

The following Figla gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Figla cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu76894	NM_012013.2 Latest version!	Mus musculus folliculogenesis specific basic helix-loop-helix (Figla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu81739	XM_017321595.1 Latest version!	Mus musculus folliculogenesis specific basic helix-loop-helix (Figla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Figla ( NM_012013.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036143.1 Mus musculus folliculogenesis specific basic helix-loop-helix (Figla), mRNA.

Price & Availability↑

CloneID	OMu76894
Clone ID Related Accession (Same CDS sequence)	NM_012013.2
Accession Version	NM_012013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	factor in the germline alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158679.8 and DV657415.1. On Nov 21, 2015 this sequence version replaced NM_012013.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U91840.1, DV657415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGATACAG CACCTGCATC CCCAGAGCCC TTCCTGGTCA CTCCGCAGGC TGAGGTGCTG 
GAGGAGCTGA TTCAGGCCCA GATGGGGCCC CTGCCCCGGC TCGCCGCCAT CTGTAGGCTC 
AAGCGCCTGC CCTCAGGCGG CTACTCCACC ACGGATGACC TGCATCTGGT GTTGGAGCGC 
AGGCGTGTGG CCAACGCCAA AGAGCGTGAA CGGATAAAAA ATCTCAACCG TGGCTTTGCC 
AAGCTGAAGG CTCTGGTGCC GTTTCTACCA CAGAGCAGGA AGCCCAGTAA AGTTGATATC 
CTTAAAGGTG CCACAGAATA CATACAGATA CTCGGCTGTG TTCTGGAAGA AGCGAAGGTC 
TCAGAGAAAC AAAGCCCTGA GGAGCAGACC CACAGTGGCA GACCCTCTGA CCCTCATGTG 
TCCTCGACTA GAGAGCTCTT GGGAAATGCT ACCCAACCTA CCAGCTGTGC GAGTGGCTTG 
AAGAAAGAAG AGGAGGGGCC TTGGGCGTAT GCTGGCCATA GTGAGCCATT GTACAGCTAC 
CATCAGAGCA CAGTACCTGA GACTAGGAGT TACTTCACTC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036143.1
CDS	55..639
Translation	MDTAPASPEPFLVTPQAEVLEELIQAQMGPLPRLAAICRLKRLPSGGYSTTDDLHLVLERRRVANAKERERIKNLNRGFAKLKALVPFLPQSRKPSKVDILKGATEYIQILGCVLEEAKVSEKQSPEEQTHSGRPSDPHVSSTRELLGNATQPTSCASGLKKEEEGPWAYAGHSEPLYSYHQSTVPETRSYFTH

Target ORF information:

RefSeq Version	NM_012013.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus folliculogenesis specific basic helix-loop-helix (Figla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012013.2

ORF Insert Sequence:

ATGGATACAG CACCTGCATC CCCAGAGCCC TTCCTGGTCA CTCCGCAGGC TGAGGTGCTG 
GAGGAGCTGA TTCAGGCCCA GATGGGGCCC CTGCCCCGGC TCGCCGCCAT CTGTAGGCTC 
AAGCGCCTGC CCTCAGGCGG CTACTCCACC ACGGATGACC TGCATCTGGT GTTGGAGCGC 
AGGCGTGTGG CCAACGCCAA AGAGCGTGAA CGGATAAAAA ATCTCAACCG TGGCTTTGCC 
AAGCTGAAGG CTCTGGTGCC GTTTCTACCA CAGAGCAGGA AGCCCAGTAA AGTTGATATC 
CTTAAAGGTG CCACAGAATA CATACAGATA CTCGGCTGTG TTCTGGAAGA AGCGAAGGTC 
TCAGAGAAAC AAAGCCCTGA GGAGCAGACC CACAGTGGCA GACCCTCTGA CCCTCATGTG 
TCCTCGACTA GAGAGCTCTT GGGAAATGCT ACCCAACCTA CCAGCTGTGC GAGTGGCTTG 
AAGAAAGAAG AGGAGGGGCC TTGGGCGTAT GCTGGCCATA GTGAGCCATT GTACAGCTAC 
CATCAGAGCA CAGTACCTGA GACTAGGAGT TACTTCACTC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Figla ( XM_017321595.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177084.1 Mus musculus folliculogenesis specific basic helix-loop-helix (Figla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81739
Clone ID Related Accession (Same CDS sequence)	XM_017321595.1
Accession Version	XM_017321595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	factor in the germline alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCCC TGCCCCGGCT CGCCGCCATC TGTAGGCTCA AGCGCCTGCC CTCAGGCGGC 
TACTCCACCA CGGATGACCT GCATCTGGTG TTGGAGCGCA GGCGTGTGGC CAACGCCAAA 
GAGCGTGAAC GGATAAAAAA TCTCAACCGT GGCTTTGCCA AGCTGAAGGC TCTGGTGCCG 
TTTCTACCAC AGAGCAGGAA GCCCAGTAAA GTTGATATCC TTAAAGGTGC CACAGAATAC 
ATACAGATAC TCGGCTGTGT TCTGGAAGAA GCGAAGGTCT CAGAGAAACA AAGCCCTGAG 
GAGCAGACCC ACAGTGGCAG ACCCTCTGAC CCTCATGTGT CCTCGACTAG AGAGCTCTTG 
GGAAATGCTA CCCAACCTAC CAGCTGTGCG AGTGGCTTGA AGAAAGAAGA GGAGGGGCCT 
TGGGCGTATG CTGGCCATAG TGAGCCATTG TACAGCTACC ATCAGAGCAC AGTACCTGAG 
ACTAGGAGTT ACTTCACTCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177084.1
CDS	561..1064
Translation	MGPLPRLAAICRLKRLPSGGYSTTDDLHLVLERRRVANAKERERIKNLNRGFAKLKALVPFLPQSRKPSKVDILKGATEYIQILGCVLEEAKVSEKQSPEEQTHSGRPSDPHVSSTRELLGNATQPTSCASGLKKEEEGPWAYAGHSEPLYSYHQSTVPETRSYFTH

Target ORF information:

RefSeq Version	XM_017321595.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus folliculogenesis specific basic helix-loop-helix (Figla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321595.1

ORF Insert Sequence:

ATGGGGCCCC TGCCCCGGCT CGCCGCCATC TGTAGGCTCA AGCGCCTGCC CTCAGGCGGC 
TACTCCACCA CGGATGACCT GCATCTGGTG TTGGAGCGCA GGCGTGTGGC CAACGCCAAA 
GAGCGTGAAC GGATAAAAAA TCTCAACCGT GGCTTTGCCA AGCTGAAGGC TCTGGTGCCG 
TTTCTACCAC AGAGCAGGAA GCCCAGTAAA GTTGATATCC TTAAAGGTGC CACAGAATAC 
ATACAGATAC TCGGCTGTGT TCTGGAAGAA GCGAAGGTCT CAGAGAAACA AAGCCCTGAG 
GAGCAGACCC ACAGTGGCAG ACCCTCTGAC CCTCATGTGT CCTCGACTAG AGAGCTCTTG 
GGAAATGCTA CCCAACCTAC CAGCTGTGCG AGTGGCTTGA AGAAAGAAGA GGAGGGGCCT 
TGGGCGTATG CTGGCCATAG TGAGCCATTG TACAGCTACC ATCAGAGCAC AGTACCTGAG 
ACTAGGAGTT ACTTCACTCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Associations among Sebox and other MEGs and its effects on early embryogenesis.
PloS one10(2)e0115050(2015)
Park MW,Kim KH,Kim EY,Lee SY,Ko JJ,Lee KA

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Simultaneous gene deletion of gata4 and gata6 leads to early disruption of follicular development and germ cell loss in the murine ovary.
Biology of reproduction91(1)24(2014 Jul)
Padua MB,Fox SC,Jiang T,Morse DA,Tevosian SG

Notch signaling regulates ovarian follicle formation and coordinates follicular growth.
Molecular endocrinology (Baltimore, Md.)28(4)499-511(2014 Apr)
Vanorny DA,Prasasya RD,Chalpe AJ,Kilen SM,Mayo KE

Figla-Cre transgenic mice expressing myristoylated EGFP in germ cells provide a model for investigating perinatal oocyte dynamics.
PloS one9(1)e84477(2014)
Lin RS,Jimenez-Movilla M,Dean J