Nt5e cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nt5e
Entrez Gene ID	23959
Full Name	5' nucleotidase, ecto
Synonyms	2210401F01Rik,5'-NT,AI447961,CD73,NT,Nt5,eNT
General protein information	Preferred Names 5' nucleotidase, ecto Names 5'-nucleotidase ecto-5'-nucleotidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.1
Summary	This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011851.4	NP_035981.1	5'-nucleotidase preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NT5E	XP_518619.3
Gallus gallus (chicken)	NT5E	XP_004940454.1
Danio rerio (zebrafish)	nt5e	NP_957226.1
Drosophila melanogaster (fruit fly)	NT5E-2	NP_611218.1
Xenopus tropicalis (tropical clawed frog)	nt5e	XP_002933973.1
Canis lupus familiaris (dog)	NT5E	XP_532221.2
Rattus norvegicus (Norway rat)	Nt5e	NP_067587.2
Drosophila melanogaster (fruit fly)	veil	NP_611217.1
Homo sapiens (human)	NT5E	NP_002517.1
	NT5E	XP_001086989.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003629	XP_313394.4
Mus musculus (house mouse)	Nt5e	NP_035981.1
Magnaporthe oryzae (rice blast fungus)	MGG_12474	XP_003715781.1
Neurospora crassa	NCU09659	XP_958725.1

Gene Ontology
Bibliography

Related articles in PubMed

Endogenous adenosine produced during hypoxia attenuates neutrophil accumulation: coordination by extracellular nucleotide metabolism.
Eltzschig HK, Thompson LF, Karhausen J, Cotta RJ, Ibla JC, Robson SC, Colgan SP
Blood104(13)3986-92(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CD73 expression identifies a subset of IgM(+) antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependent
Title: CD73 expression identifies a subset of IgM

findings demonstrate the critical role of CD73 in the regulation of infection by the enteric pathogen Salmonella and implicate adenosine as an important host-derived factor that mediates host-microbe interaction
Title: Intestinal Epithelial Ecto-5'-Nucleotidase (CD73) Regulates Intestinal Colonization and Infection by Nontyphoidal Salmonella.

CD73 on T cells plays an important anti-inflammatory role in transverse aortic constriction-induced heart failure
Title: Adenosine Formed by CD73 on T Cells Inhibits Cardiac Inflammation and Fibrosis and Preserves Contractile Function in Transverse Aortic Constriction-Induced Heart Failure.

Deletion of CD39 and CD73 or both caused an inhibition of the microglia ramified phenotype in the brain with a reduction in the length of processes, branching frequency and number of intersections with Sholl spheres. In vitro, unlike wild-type microglia, cd39-/- and cd73-/- microglial cells were less complex and did not respond to ATP with the transformation into a more ramified phenotype.
Title: The adenosine generating enzymes CD39/CD73 control microglial processes ramification in the mouse brain.

Data show that AMP deaminase 3 (Ampd3)-/-/5'-nucleotidase (CD73)-/- knockout mice are more sensitive to AMP-induced hypometabolism than mice with a single enzyme deficiency, which are more sensitive than wild type.
Title: New insights on the regulation of the adenine nucleotide pool of erythrocytes in mouse models.

The following Nt5e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nt5e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20410	NM_011851.4 Latest version!	Mus musculus 5' nucleotidase, ecto (Nt5e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$335.30 $479.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nt5e ( NM_011851.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035981.1 Mus musculus 5' nucleotidase, ecto (Nt5e), mRNA.

Price & Availability↑

CloneID	OMu20410
Clone ID Related Accession (Same CDS sequence)	NM_011851.4
Accession Version	NM_011851.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	5'-nucleotidase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB850214.1 and AK029979.1. On Mar 13, 2010 this sequence version replaced NM_011851.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029979.1, AK154614.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTCCCG CGGCCGCTAA GGTACCCAAG TGGCTGCTTC TCGCACTGAG CGCTCTACTA 
CCACAGTGGC CCGCAGCCAG TGCCTGGGAG CTCACGATCC TGCACACAAA CGACGTGCAC 
AGCCGGCTAG AGCAGACCAG CGATGACTCC ACCAAGTGCC TCAACGCCAG CCTGTGTGTG 
GGCGGCGTGG CCCGGCTCTT TACCAAGGTG CAGCAGATCC GCAAGGAAGA ACCCAACGTG 
CTGTTTTTGG ATGCCGGAGA CCAGTACCAG GGCACCATCT GGTTCACCGT TTACAAAGGC 
CTTGAAGTGG CACACTTCAT GAACATCCTG GGCTACGATG CTATGGCACT GGGAAATCAT 
GAATTTGATA ACGGTGTGGA AGGACTGATT GATCCCCTCC TCAGAAACGT TAAATTTCCA 
ATTCTGAGCG CAAACATTAA GGCACGGGGG CCTCTAGCAC ATCAGATATC TGGACTTTTT 
CTGCCATCTA AAGTTCTCTC TGTTGGCGGT GAGGTTGTGG GGATTGTTGG ATATACTTCA 
AAGGAAACCC CTTTCCTCTC AAATCCAGGG ACAAATTTAG TCTTTGAAGA TGAAATCTCT 
GCATTGCAGC CTGAAGTAGA TAAACTAAAG ACGCTAAATG TGAATAAGAT CATCGCCCTG 
GGGCACTCTG GTTTTGAGAT GGACAAACTT ATCGCTCAGA AAGTTCGAGG TGTGGACATC 
GTGGTGGGAG GACACTCCAA CACCTTTCTC TACACAGGAA ATCCACCTTC CAAAGAAGTG 
CCTGCGGGGA AGTACCCATT CATAGTCACC GCAGATGATG GACGGCAGGT GCCTGTGGTC 
CAGGCCTATG CCTTTGGCAA ATACCTGGGC TACCTGAAGG TTGAGTTTGA TGATAAAGGC 
AATGTTATCA CTTCCTATGG AAATCCCATT CTTCTCAACA GCAGCATTCC TGAAGATGCG 
ACCATCAAAG CAGACATTAA CCAATGGAGG ATAAAATTAG ATAATTATTC TACCCAGGAA 
CTCGGGAGAA CGATCGTCTA CCTGGATGGC TCCACTCAGA CGTGCCGCTT CAGGGAATGC 
AACATGGGAA ACCTGATCTG TGATGCCATG ATTAACAACA ACCTCAGACA CCCAGATGAA 
ATGTTTTGGA ACCACGTGTC CATGTGCATT GTAAACGGAG GTGGCATCCG GTCCCCCATT 
GATGAGAAGA ACAATGGTAC CATCACCTGG GAGAACCTGG CTGCTGTGCT GCCCTTTGGA 
GGGACATTTG ACCTCGTCCA ATTAAAAGGG TCCACCCTGA AGAAGGCTTT TGAGCACAGC 
GTGCATCGCT ATGGCCAGTC CACCGGAGAG TTCCTGCAAG TGGGTGGAAT CCATGTGGTG 
TACGATATTA ACCGAAAGCC CTGGAACAGA GTGGTCCAAT TAGAAGTTCT CTGCACCAAG 
TGTCGAGTGC CCATCTATGA GCCTCTTGAA ATGGATAAAG TGTATAAAGT GACCCTCCCA 
AGCTATCTGG CCAACGGTGG AGATGGATTC CAGATGATAA AAGATGAATT ACTAAAGCAT 
GACTCTGGTG ATCAAGATAT CAGCGTGGTT TCTGAATACA TCTCAAAAAT GAAAGTAGTT 
TACCCAGCCG TTGAAGGGCG GATCAAGTTC TCTGCAGCAA GTCATTACCA GGGAAGCTTT 
CCTTTAGTAA TTCTTTCATT TTGGGCAATG ATCCTTATTT TGTACCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035981.1
CDS	78..1808
Translation	MRPAAAKVPKWLLLALSALLPQWPAASAWELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKFSAASHYQGSFPLVILSFWAMILILYQ

Target ORF information:

RefSeq Version	NM_011851.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5' nucleotidase, ecto (Nt5e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011851.4

ORF Insert Sequence:

ATGCGTCCCG CGGCCGCTAA GGTACCCAAG TGGCTGCTTC TCGCACTGAG CGCTCTACTA 
CCACAGTGGC CCGCAGCCAG TGCCTGGGAG CTCACGATCC TGCACACAAA CGACGTGCAC 
AGCCGGCTAG AGCAGACCAG CGATGACTCC ACCAAGTGCC TCAACGCCAG CCTGTGTGTG 
GGCGGCGTGG CCCGGCTCTT TACCAAGGTG CAGCAGATCC GCAAGGAAGA ACCCAACGTG 
CTGTTTTTGG ATGCCGGAGA CCAGTACCAG GGCACCATCT GGTTCACCGT TTACAAAGGC 
CTTGAAGTGG CACACTTCAT GAACATCCTG GGCTACGATG CTATGGCACT GGGAAATCAT 
GAATTTGATA ACGGTGTGGA AGGACTGATT GATCCCCTCC TCAGAAACGT TAAATTTCCA 
ATTCTGAGCG CAAACATTAA GGCACGGGGG CCTCTAGCAC ATCAGATATC TGGACTTTTT 
CTGCCATCTA AAGTTCTCTC TGTTGGCGGT GAGGTTGTGG GGATTGTTGG ATATACTTCA 
AAGGAAACCC CTTTCCTCTC AAATCCAGGG ACAAATTTAG TCTTTGAAGA TGAAATCTCT 
GCATTGCAGC CTGAAGTAGA TAAACTAAAG ACGCTAAATG TGAATAAGAT CATCGCCCTG 
GGGCACTCTG GTTTTGAGAT GGACAAACTT ATCGCTCAGA AAGTTCGAGG TGTGGACATC 
GTGGTGGGAG GACACTCCAA CACCTTTCTC TACACAGGAA ATCCACCTTC CAAAGAAGTG 
CCTGCGGGGA AGTACCCATT CATAGTCACC GCAGATGATG GACGGCAGGT GCCTGTGGTC 
CAGGCCTATG CCTTTGGCAA ATACCTGGGC TACCTGAAGG TTGAGTTTGA TGATAAAGGC 
AATGTTATCA CTTCCTATGG AAATCCCATT CTTCTCAACA GCAGCATTCC TGAAGATGCG 
ACCATCAAAG CAGACATTAA CCAATGGAGG ATAAAATTAG ATAATTATTC TACCCAGGAA 
CTCGGGAGAA CGATCGTCTA CCTGGATGGC TCCACTCAGA CGTGCCGCTT CAGGGAATGC 
AACATGGGAA ACCTGATCTG TGATGCCATG ATTAACAACA ACCTCAGACA CCCAGATGAA 
ATGTTTTGGA ACCACGTGTC CATGTGCATT GTAAACGGAG GTGGCATCCG GTCCCCCATT 
GATGAGAAGA ACAATGGTAC CATCACCTGG GAGAACCTGG CTGCTGTGCT GCCCTTTGGA 
GGGACATTTG ACCTCGTCCA ATTAAAAGGG TCCACCCTGA AGAAGGCTTT TGAGCACAGC 
GTGCATCGCT ATGGCCAGTC CACCGGAGAG TTCCTGCAAG TGGGTGGAAT CCATGTGGTG 
TACGATATTA ACCGAAAGCC CTGGAACAGA GTGGTCCAAT TAGAAGTTCT CTGCACCAAG 
TGTCGAGTGC CCATCTATGA GCCTCTTGAA ATGGATAAAG TGTATAAAGT GACCCTCCCA 
AGCTATCTGG CCAACGGTGG AGATGGATTC CAGATGATAA AAGATGAATT ACTAAAGCAT 
GACTCTGGTG ATCAAGATAT CAGCGTGGTT TCTGAATACA TCTCAAAAAT GAAAGTAGTT 
TACCCAGCCG TTGAAGGGCG GATCAAGTTC TCTGCAGCAA GTCATTACCA GGGAAGCTTT 
CCTTTAGTAA TTCTTTCATT TTGGGCAATG ATCCTTATTT TGTACCAATA A