Zc3h12d cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zc3h12d
Entrez Gene ID	237256
Full Name	zinc finger CCCH type containing 12D
Synonyms	D730019B10Rik,TFL
General protein information	Preferred Names zinc finger CCCH type containing 12D Names probable ribonuclease ZC3H12D transformed follicular lymphoma homolog zc3h12d
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512723.3	XP_006512786.1	probable ribonuclease ZC3H12D isoform X1
XM_006512723.4	XP_006512786.1	probable ribonuclease ZC3H12D isoform X1
XM_006512724.2	XP_006512787.1	probable ribonuclease ZC3H12D isoform X1
XM_030245080.1	XP_030100940.1	probable ribonuclease ZC3H12D isoform X2
NM_172785.3	NP_766373.2	probable ribonuclease ZC3H12D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ZC3H12D	XP_541146.3
Rattus norvegicus (Norway rat)	Zc3h12d	NP_001100939.1
	ZC3H12D	XP_001087043.1
Homo sapiens (human)	ZC3H12D	NP_997243.2
Bos taurus (cattle)	ZC3H12D	XP_002690367.1
Pan troglodytes (chimpanzee)	ZC3H12D	XP_518794.2
Mus musculus (house mouse)	Zc3h12d	NP_766373.2

Gene Ontology
Bibliography

Related articles in PubMed

ZC3H12D attenuated inflammation responses by reducing mRNA stability of proinflammatory genes.
Zhang H, Wang WC, Chen JK, Zhou L, Wang M, Wang ZD, Yang B, Xia YM, Lei S, Fu EQ, Jiang T
Molecular immunology67(2 Pt B)206-12(2015 Oct)

Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.
Huang S, Liu S, Fu JJ, Tony Wang T, Yao X, Kumar A, Liu G, Fu M
The Journal of biological chemistry290(34)20782-92(2015 Aug)

Posttranscriptional modulation of cytokine production in T cells for the regulation of excessive inflammation by TFL.
Minagawa K, Wakahashi K, Kawano H, Nishikawa S, Fukui C, Kawano Y, Asada N, Sato M, Sada A, Katayama Y, Matsui T
Journal of immunology (Baltimore, Md. : 1950)192(4)1512-24(2014 Feb)

The putative tumor suppressor Zc3h12d modulates toll-like receptor signaling in macrophages.
Huang S, Qi D, Liang J, Miao R, Minagawa K, Quinn T, Matsui T, Fan D, Liu J, Fu M
Cellular signalling24(2)569-76(2012 Feb)

Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
Liang J, Song W, Tromp G, Kolattukudy PE, Fu M
PloS one3(8)e2880(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data indicated that ZC3H12D could suppress both the initial inflammation storm and chronic inflammation by targeting the mRNA of cytokines as well as NF-kappaB and c-fos.
Title: ZC3H12D attenuated inflammation responses by reducing mRNA stability of proinflammatory genes.

TFL (Zc3h12d) may play an important role in attenuating local inflammation by suppressing the infiltration of Th17 cells in the central nervous system during the resolution phase of experimental autoimmune encephalitis.
Title: Posttranscriptional modulation of cytokine production in T cells for the regulation of excessive inflammation by TFL.

Zc3h12d is a novel negative feedback regulator of TLR signaling and macrophage activation and thus may play a role in host immunity and inflammatory diseases.
Title: The putative tumor suppressor Zc3h12d modulates toll-like receptor signaling in macrophages.

MCPIP1, 2, 3, and 4, encoded by four genes, Zc3h12a, Zc3h12b, Zc3h12c, and Zc3h12d, respectively, regulates macrophage activation.
Title: A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages.

The following Zc3h12d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zc3h12d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18303	NM_172785.3 Latest version!	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu18303	XM_006512724.2 Latest version!	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu85445	XM_030245080.1 Latest version!	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu18303	XM_006512723.4 Latest version!	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu18303	XM_006512723.3	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zc3h12d ( NM_172785.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766373.2 Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), mRNA.

Price & Availability↑

CloneID	OMu18303
Clone ID Related Accession (Same CDS sequence)	XM_006512723.3 , XM_006512723.4 , XM_006512724.2 , NM_172785.3
Accession Version	NM_172785.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	probable ribonuclease ZC3H12D
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB661745.1, AK052806.1 and AC092856.26. On Apr 7, 2009 this sequence version replaced NM_172785.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK052806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766373.2
CDS	164..1765
Translation	MEHRSKMEFFQKLGYSQEDVVRVLGKLGDSALVNDVLQELIQTGSRPRAQEDPASGTGVVLIPRGCCGVQDSAQQGLGPGLEEAGGDPARFLRPIVIDGSNVAMSHGNKEAFSCRGIRLAVDWFRDRGHTYIKVFVPSWRKEPSRSDTPIREQHVLEELERQAVLVYTPSRKVNGKRVVCYDDRYIVKVAYEKDGIIVSNDNYRDLQNENPEWKWFIEQRLLMFSFVNDRFMPPDDPLGRRGPTLSNFLSKKPRPPEPSWQHCPYGKKCTYGVKCRFYHPERPHHGQLSVADELRAKTRAWLGGGAEEPRTPSARSRPTTARLLPQEPGEHDLPPAPQPAVLAALNRSFARLTFSDTAASGVVSQSRGPDWMPTGVPTSWAPPSLRAGSAATIGLPGMRSLRTPNNPLSPGDLGSPICPQARLSERHRSRDMHSDLPPQRRPLEDPWALLPSSYCYLNHSVWSESAWGEDIFRGPSESAQPVANGGGTRPVHCSFFPPDQDHPVMASGPPLSDMALLTLLQRSQKTGAPLGDP

Target ORF information:

RefSeq Version	NM_172785.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172785.3

ORF Insert Sequence:

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zc3h12d ( XM_006512723.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512786.1 Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18303
Clone ID Related Accession (Same CDS sequence)	XM_006512723.3 , XM_006512723.4 , XM_006512724.2 , NM_172785.3
Accession Version	XM_006512723.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ribonuclease ZC3H12D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512786.1
CDS	219..1820
Translation	MEHRSKMEFFQKLGYSQEDVVRVLGKLGDSALVNDVLQELIQTGSRPRAQEDPASGTGVVLIPRGCCGVQDSAQQGLGPGLEEAGGDPARFLRPIVIDGSNVAMSHGNKEAFSCRGIRLAVDWFRDRGHTYIKVFVPSWRKEPSRSDTPIREQHVLEELERQAVLVYTPSRKVNGKRVVCYDDRYIVKVAYEKDGIIVSNDNYRDLQNENPEWKWFIEQRLLMFSFVNDRFMPPDDPLGRRGPTLSNFLSKKPRPPEPSWQHCPYGKKCTYGVKCRFYHPERPHHGQLSVADELRAKTRAWLGGGAEEPRTPSARSRPTTARLLPQEPGEHDLPPAPQPAVLAALNRSFARLTFSDTAASGVVSQSRGPDWMPTGVPTSWAPPSLRAGSAATIGLPGMRSLRTPNNPLSPGDLGSPICPQARLSERHRSRDMHSDLPPQRRPLEDPWALLPSSYCYLNHSVWSESAWGEDIFRGPSESAQPVANGGGTRPVHCSFFPPDQDHPVMASGPPLSDMALLTLLQRSQKTGAPLGDP

Target ORF information:

RefSeq Version	XM_006512723.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512723.3

ORF Insert Sequence:

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zc3h12d ( XM_006512724.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512787.1 Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu18303
Clone ID Related Accession (Same CDS sequence)	XM_006512723.3 , XM_006512723.4 , XM_006512724.2 , NM_172785.3
Accession Version	XM_006512724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable ribonuclease ZC3H12D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006512724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512787.1
CDS	90..1691
Translation	MEHRSKMEFFQKLGYSQEDVVRVLGKLGDSALVNDVLQELIQTGSRPRAQEDPASGTGVVLIPRGCCGVQDSAQQGLGPGLEEAGGDPARFLRPIVIDGSNVAMSHGNKEAFSCRGIRLAVDWFRDRGHTYIKVFVPSWRKEPSRSDTPIREQHVLEELERQAVLVYTPSRKVNGKRVVCYDDRYIVKVAYEKDGIIVSNDNYRDLQNENPEWKWFIEQRLLMFSFVNDRFMPPDDPLGRRGPTLSNFLSKKPRPPEPSWQHCPYGKKCTYGVKCRFYHPERPHHGQLSVADELRAKTRAWLGGGAEEPRTPSARSRPTTARLLPQEPGEHDLPPAPQPAVLAALNRSFARLTFSDTAASGVVSQSRGPDWMPTGVPTSWAPPSLRAGSAATIGLPGMRSLRTPNNPLSPGDLGSPICPQARLSERHRSRDMHSDLPPQRRPLEDPWALLPSSYCYLNHSVWSESAWGEDIFRGPSESAQPVANGGGTRPVHCSFFPPDQDHPVMASGPPLSDMALLTLLQRSQKTGAPLGDP

Target ORF information:

RefSeq Version	XM_006512724.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512724.2

ORF Insert Sequence:

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zc3h12d ( XM_030245080.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100940.1 Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X3, mRNA.

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CloneID	OMu85445
Clone ID Related Accession (Same CDS sequence)	XM_030245080.1
Accession Version	XM_030245080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable ribonuclease ZC3H12D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCT TTGTAAATGA CAGGTTCATG CCTCCTGATG ACCCCCTGGG CCGCCGTGGA 
CCAACACTAA GCAACTTCCT AAGCAAGAAG CCAAGGCCCC CAGAGCCATC CTGGCAACAC 
TGTCCCTATG GCAAGAAATG CACCTACGGG GTCAAGTGCA GATTCTACCA CCCCGAGAGA 
CCACACCATG GGCAGCTGTC TGTGGCCGAC GAACTCCGTG CCAAGACGCG GGCCTGGCTG 
GGTGGTGGCG CCGAGGAGCC GCGGACACCG AGCGCCCGGA GCCGTCCTAC AACAGCCCGG 
CTGCTTCCCC AGGAGCCCGG CGAGCACGAC CTCCCGCCAG CCCCGCAGCC CGCGGTCCTG 
GCAGCTTTGA ATAGGAGCTT CGCTCGGCTC ACCTTCAGCG ACACCGCGGC CAGCGGCGTC 
GTGTCTCAGT CTCGTGGGCC GGACTGGATG CCCACGGGTG TCCCCACGTC CTGGGCCCCA 
CCATCCCTTC GCGCGGGCAG CGCTGCCACC ATAGGGTTGC CAGGTATGCG GAGCCTCCGG 
ACTCCGAACA ACCCGCTTTC TCCGGGCGAT CTCGGGTCTC CAATCTGCCC ACAGGCCCGG 
CTGTCGGAGA GACACCGTTC CAGGGACATG CACAGTGACC TGCCTCCCCA GCGCAGACCG 
CTGGAAGATC CGTGGGCTCT CCTGCCCAGC TCCTATTGTT ATCTGAACCA CTCGGTCTGG 
TCCGAGTCGG CTTGGGGTGA GGACATCTTT AGAGGACCTT CGGAGTCAGC ACAACCTGTC 
GCCAATGGTG GGGGAACACG CCCTGTGCAC TGCAGCTTCT TCCCACCTGA CCAAGACCAC 
CCTGTGATGG CCTCGGGTCC TCCGCTCTCC GACATGGCTC TGCTCACCCT CCTGCAGAGA 
TCCCAAAAGA CCGGTGCTCC GTTGGGGGAT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100940.1
CDS	485..1420
Translation	MFSFVNDRFMPPDDPLGRRGPTLSNFLSKKPRPPEPSWQHCPYGKKCTYGVKCRFYHPERPHHGQLSVADELRAKTRAWLGGGAEEPRTPSARSRPTTARLLPQEPGEHDLPPAPQPAVLAALNRSFARLTFSDTAASGVVSQSRGPDWMPTGVPTSWAPPSLRAGSAATIGLPGMRSLRTPNNPLSPGDLGSPICPQARLSERHRSRDMHSDLPPQRRPLEDPWALLPSSYCYLNHSVWSESAWGEDIFRGPSESAQPVANGGGTRPVHCSFFPPDQDHPVMASGPPLSDMALLTLLQRSQKTGAPLGDP

Target ORF information:

RefSeq Version	XM_030245080.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245080.1

ORF Insert Sequence:

ATGTTCTCCT TTGTAAATGA CAGGTTCATG CCTCCTGATG ACCCCCTGGG CCGCCGTGGA 
CCAACACTAA GCAACTTCCT AAGCAAGAAG CCAAGGCCCC CAGAGCCATC CTGGCAACAC 
TGTCCCTATG GCAAGAAATG CACCTACGGG GTCAAGTGCA GATTCTACCA CCCCGAGAGA 
CCACACCATG GGCAGCTGTC TGTGGCCGAC GAACTCCGTG CCAAGACGCG GGCCTGGCTG 
GGTGGTGGCG CCGAGGAGCC GCGGACACCG AGCGCCCGGA GCCGTCCTAC AACAGCCCGG 
CTGCTTCCCC AGGAGCCCGG CGAGCACGAC CTCCCGCCAG CCCCGCAGCC CGCGGTCCTG 
GCAGCTTTGA ATAGGAGCTT CGCTCGGCTC ACCTTCAGCG ACACCGCGGC CAGCGGCGTC 
GTGTCTCAGT CTCGTGGGCC GGACTGGATG CCCACGGGTG TCCCCACGTC CTGGGCCCCA 
CCATCCCTTC GCGCGGGCAG CGCTGCCACC ATAGGGTTGC CAGGTATGCG GAGCCTCCGG 
ACTCCGAACA ACCCGCTTTC TCCGGGCGAT CTCGGGTCTC CAATCTGCCC ACAGGCCCGG 
CTGTCGGAGA GACACCGTTC CAGGGACATG CACAGTGACC TGCCTCCCCA GCGCAGACCG 
CTGGAAGATC CGTGGGCTCT CCTGCCCAGC TCCTATTGTT ATCTGAACCA CTCGGTCTGG 
TCCGAGTCGG CTTGGGGTGA GGACATCTTT AGAGGACCTT CGGAGTCAGC ACAACCTGTC 
GCCAATGGTG GGGGAACACG CCCTGTGCAC TGCAGCTTCT TCCCACCTGA CCAAGACCAC 
CCTGTGATGG CCTCGGGTCC TCCGCTCTCC GACATGGCTC TGCTCACCCT CCTGCAGAGA 
TCCCAAAAGA CCGGTGCTCC GTTGGGGGAT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zc3h12d ( XM_006512723.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512786.1 Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X1, mRNA.

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CloneID	OMu18303
Clone ID Related Accession (Same CDS sequence)	XM_006512723.3 , XM_006512723.4 , XM_006512724.2 , NM_172785.3
Accession Version	XM_006512723.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable ribonuclease ZC3H12D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512723.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512786.1
CDS	214..1815
Translation	MEHRSKMEFFQKLGYSQEDVVRVLGKLGDSALVNDVLQELIQTGSRPRAQEDPASGTGVVLIPRGCCGVQDSAQQGLGPGLEEAGGDPARFLRPIVIDGSNVAMSHGNKEAFSCRGIRLAVDWFRDRGHTYIKVFVPSWRKEPSRSDTPIREQHVLEELERQAVLVYTPSRKVNGKRVVCYDDRYIVKVAYEKDGIIVSNDNYRDLQNENPEWKWFIEQRLLMFSFVNDRFMPPDDPLGRRGPTLSNFLSKKPRPPEPSWQHCPYGKKCTYGVKCRFYHPERPHHGQLSVADELRAKTRAWLGGGAEEPRTPSARSRPTTARLLPQEPGEHDLPPAPQPAVLAALNRSFARLTFSDTAASGVVSQSRGPDWMPTGVPTSWAPPSLRAGSAATIGLPGMRSLRTPNNPLSPGDLGSPICPQARLSERHRSRDMHSDLPPQRRPLEDPWALLPSSYCYLNHSVWSESAWGEDIFRGPSESAQPVANGGGTRPVHCSFFPPDQDHPVMASGPPLSDMALLTLLQRSQKTGAPLGDP

Target ORF information:

RefSeq Version	XM_006512723.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger CCCH type containing 12D (Zc3h12d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512723.4

ORF Insert Sequence:

ATGGAGCATC GGAGCAAGAT GGAATTTTTC CAGAAACTGG GCTACAGCCA GGAGGACGTG 
GTCAGGGTGC TGGGCAAACT GGGTGACAGT GCCTTGGTCA ATGACGTGCT TCAAGAGTTG 
ATCCAGACCG GCAGTCGCCC AAGGGCCCAG GAGGATCCAG CCAGTGGCAC CGGGGTAGTG 
CTGATTCCCC GGGGATGCTG CGGGGTCCAA GACTCTGCCC AGCAGGGCCT GGGACCAGGC 
CTAGAAGAGG CTGGCGGAGA TCCAGCCCGC TTCCTGCGGC CCATAGTGAT CGATGGCAGC 
AATGTGGCTA TGAGCCATGG AAATAAAGAA GCCTTCTCTT GCCGGGGAAT TCGTCTGGCT 
GTGGACTGGT TTAGAGACAG AGGACACACC TACATCAAGG TTTTTGTCCC ATCTTGGAGG 
AAAGAGCCAT CAAGGTCTGA TACTCCTATC AGAGAGCAAC ACGTGCTGGA GGAACTGGAG 
CGGCAAGCTG TGTTGGTGTA TACCCCATCC CGCAAGGTGA ACGGCAAGCG AGTGGTCTGC 
TACGATGACC GCTACATCGT GAAGGTGGCC TATGAGAAGG ATGGCATCAT TGTCTCCAAT 
GACAACTACC GAGACCTGCA GAACGAAAAC CCAGAGTGGA AATGGTTCAT CGAGCAGCGG 
CTGCTCATGT TCTCCTTTGT AAATGACAGG TTCATGCCTC CTGATGACCC CCTGGGCCGC 
CGTGGACCAA CACTAAGCAA CTTCCTAAGC AAGAAGCCAA GGCCCCCAGA GCCATCCTGG 
CAACACTGTC CCTATGGCAA GAAATGCACC TACGGGGTCA AGTGCAGATT CTACCACCCC 
GAGAGACCAC ACCATGGGCA GCTGTCTGTG GCCGACGAAC TCCGTGCCAA GACGCGGGCC 
TGGCTGGGTG GTGGCGCCGA GGAGCCGCGG ACACCGAGCG CCCGGAGCCG TCCTACAACA 
GCCCGGCTGC TTCCCCAGGA GCCCGGCGAG CACGACCTCC CGCCAGCCCC GCAGCCCGCG 
GTCCTGGCAG CTTTGAATAG GAGCTTCGCT CGGCTCACCT TCAGCGACAC CGCGGCCAGC 
GGCGTCGTGT CTCAGTCTCG TGGGCCGGAC TGGATGCCCA CGGGTGTCCC CACGTCCTGG 
GCCCCACCAT CCCTTCGCGC GGGCAGCGCT GCCACCATAG GGTTGCCAGG TATGCGGAGC 
CTCCGGACTC CGAACAACCC GCTTTCTCCG GGCGATCTCG GGTCTCCAAT CTGCCCACAG 
GCCCGGCTGT CGGAGAGACA CCGTTCCAGG GACATGCACA GTGACCTGCC TCCCCAGCGC 
AGACCGCTGG AAGATCCGTG GGCTCTCCTG CCCAGCTCCT ATTGTTATCT GAACCACTCG 
GTCTGGTCCG AGTCGGCTTG GGGTGAGGAC ATCTTTAGAG GACCTTCGGA GTCAGCACAA 
CCTGTCGCCA ATGGTGGGGG AACACGCCCT GTGCACTGCA GCTTCTTCCC ACCTGACCAA 
GACCACCCTG TGATGGCCTC GGGTCCTCCG CTCTCCGACA TGGCTCTGCT CACCCTCCTG 
CAGAGATCCC AAAAGACCGG TGCTCCGTTG GGGGATCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZC3H12D attenuated inflammation responses by reducing mRNA stability of proinflammatory genes.
Molecular immunology67(2 Pt B)206-12(2015 Oct)
Zhang H,Wang WC,Chen JK,Zhou L,Wang M,Wang ZD,Yang B,Xia YM,Lei S,Fu EQ,Jiang T

Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.
The Journal of biological chemistry290(34)20782-92(2015 Aug)
Huang S,Liu S,Fu JJ,Tony Wang T,Yao X,Kumar A,Liu G,Fu M

Posttranscriptional modulation of cytokine production in T cells for the regulation of excessive inflammation by TFL.
Journal of immunology (Baltimore, Md. : 1950)192(4)1512-24(2014 Feb)
Minagawa K,Wakahashi K,Kawano H,Nishikawa S,Fukui C,Kawano Y,Asada N,Sato M,Sada A,Katayama Y,Matsui T

The putative tumor suppressor Zc3h12d modulates toll-like receptor signaling in macrophages.
Cellular signalling24(2)569-76(2012 Feb)
Huang S,Qi D,Liang J,Miao R,Minagawa K,Quinn T,Matsui T,Fan D,Liu J,Fu M

Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
PloS one3(8)e2880(2008 Aug)
Liang J,Song W,Tromp G,Kolattukudy PE,Fu M

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Zc3h12d cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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