Spire2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spire2
Entrez Gene ID	234857
Full Name	spire type actin nucleation factor 2
Synonyms	BC026502,Spir-2,Spir2
General protein information	Preferred Names spire type actin nucleation factor 2 Names protein spire homolog 2 spire homolog 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_172287.2	NP_758491.1	protein spire homolog 2

Pathways from BioSystems

Homologies

	SPIRE2	XP_002802641.1
Rattus norvegicus (Norway rat)	Spire2	NP_001121010.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008101	XP_317355.4
Danio rerio (zebrafish)	spire2	NP_001007343.1
Xenopus tropicalis (tropical clawed frog)	spire2	XP_002933730.2
Homo sapiens (human)	SPIRE2	NP_115827.1
Canis lupus familiaris (dog)	SPIRE2	XP_003639110.2
Gallus gallus (chicken)	SPIRE2	XP_004944379.1
Pan troglodytes (chimpanzee)	SPIRE2	XP_511239.4
Mus musculus (house mouse)	Spire2	NP_758491.1

Gene Ontology
Bibliography

Related articles in PubMed

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.
Pylypenko O, Welz T, Tittel J, Kollmar M, Chardon F, Malherbe G, Weiss S, Michel CI, Samol-Wolf A, Grasskamp AT, Hume A, Goud B, Baron B, England P, Titus MA, Schwille P, Weidemann T, Houdusse A, Kerkhoff E
eLife5(2016 09)

Enhanced fear expression in Spir-1 actin organizer mutant mice.
Pleiser S, Banchaabouchi MA, Samol-Wolf A, Farley D, Welz T, Wellbourne-Wood J, Gehring I, Linkner J, Faix J, Riemenschneider MJ, Dietrich S, Kerkhoff E
European journal of cell biology93(5-6)225-37(2014 May-Jun)

Spir2; a novel QTL on chromosome 4 contributes to susceptibility to pneumococcal infection in mice.
Wisby L, Fernandes VE, Neill DR, Kadioglu A, Andrew PW, Denny P
BMC genomics14242(2013 Apr)

An actin-dependent mechanism for long-range vesicle transport.
Schuh M
Nature cell biology13(12)1431-6(2011 Oct)

Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.
Pfender S, Kuznetsov V, Pleiser S, Kerkhoff E, Schuh M
Current biology : CB21(11)955-60(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors determined crystal structures of MyoVa-globular tail domain bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form.
Title: Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.

We describe a novel locus, called Streptococcus pneumoniae infection resistance 2 (Spir2) on Chr4, which influences time to morbidity and the development of bacteraemia post-infection.
Title: Spir2; a novel QTL on chromosome 4 contributes to susceptibility to pneumococcal infection in mice.

Spire1 and Spire2 drive two critical steps of asymmetric oocyte division
Title: Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.

Spir-2 is found to be expressed as a single mRNA in the developing nervous system and intestine. In adult mouse it is expressed in epithelial cells of the digestive tract, in neuronal cells of the nervous system, and in testical spermatocytes.
Title: Expression patterns of the mouse Spir-2 actin nucleator.

The following Spire2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spire2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14567	NM_172287.2 Latest version!	Mus musculus spire type actin nucleation factor 2 (Spire2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$419.30 $599.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spire2 ( NM_172287.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_758491.1 Mus musculus spire type actin nucleation factor 2 (Spire2), mRNA.

Price & Availability↑

CloneID	OMu14567
Clone ID Related Accession (Same CDS sequence)	NM_172287.2
Accession Version	NM_172287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Mus musculus(house mouse)
Product	protein spire homolog 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049152.1. On Jun 19, 2003 this sequence version replaced NM_172287.1. ##Evidence-Data-START## Transcript exon combination :: BC049152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCGGG CGGGCGGCGG CGGCGCAGCA GCTCCCGAAC GCGCGGGGGG CGCGGCGCGG 
CCCGAGCCGT GGGAGCTGTC CCTGGAGGAA GTGCTGAAGG TGTACGAGCA GCCCATCAAC 
GAGGAGCAGG CCTGGGCGGT GTGCTTCCAG GGCTGCCGCG GGCTGCGGGG CGAGCCGGGC 
GGCGTGCGGC GCATCCGGGA CACGGCAGAC ATCCTCCTGC GTAGGGACGG TTCGGTTGGC 
GCGCGGCTGG AGCCCGAGCC TACAACCATG GTGGTACCAC CAGCCAGCTC AGAAGCCCAG 
ATGGTGCAGT CACTGGGCTT TGCCATTTAC CGTGCGCTGG ACTGGGGGTT AGATGAGAAC 
GAGGAGAGAG AGCTCAGCCC GCAGCTGGAA AGGCTCATCG ACCTCATGGC CAACAGCGAC 
TGCGAAGACA GCAGCTGCGG GGCAGCGGAT GAGGGCTACG TGGGTCCAGA AGAGGAGGAG 
GAGGCTGAGG GCGGCCCCCG AGCTGTGCGC ACCTTTGCCC AGGCTATGCG GCTGTGTGCT 
TTGCGCCTGA CCGACCCCCA CGGCGCGCAG GCCCACTACC AGGCAGTGTG CCGAGCTCTC 
TTTGTGGAGA CACTGGAGCT TCGTGCCTTC CTGGCCCGGG TGAGGGAGGC CAAAGAGATG 
CTGAAGAAAC TGGGGGAGGA GGAGCCACGG GAGAAGCCAC TGGCCGAGCT CGACCACCTG 
GGACACACAG ACTGGGCCCG GCTGTGGGTC CAGCTCATGC GGGAGCTCCG TCACGGGGTA 
AAGCTGAAGA AGGTGCAGGA GAAGGAGTTC AACCCACTGC CCACCGAGTT CCAGCTCACC 
CCCTTCGAGA TGCTGATGCA GGATATCCGT GCCAGGAACT ATAAGCTGCG CAAGGTCATG 
GTCGATGGGG ACATCCCTCC CAGGGTGAAG AAGGACGCAC ATGAACTTAT CCTGGACTTC 
ATTCGTTCTC GGCCTCCACT GAAGCAGGTC TCAGAGAGAC AACTTCGCCC TGTACCACAG 
AAGCAAAGAA CACTGCACGA GAAGATCCTG GAGGAAATCA AGCAGGAGCG GAGGCTTCGT 
CCAGTCGGGG CCCAGCACTT GGGAGGCCGT GGGTTTGGTT CCCTGCCCTG CATCCTCAAT 
GCCTGCTCTG GGGACATCAA GTCCACTTCC TGCATCAACC TGTCTGTCAC CGATACTGGG 
AGCGGGAGTC AGCGCCCACG GCCCCGGGTC CTGCTCAAAG CCCCCACCCT TGCAGAGATG 
GAAGAAATGA ACACGTCTGA GGAAGAAGAG TCCCCATGTG GGGAGGTGGC ACTGAAGCGG 
GACCGCTCTT TTTCGGAACA CGACCTGGCC CAGCTTCGGA GCGAGATGGC ATCTGGCCTT 
CAGTCAGCCG CTCAGCCCCC AGGAGGGACG GAGCCACCTC GGGCCAGAGC AGGTAGCATG 
CACTCCTGGA GGCCCAGCAG CCGAGACCAG GGTTTCTGTC CTGTGAGCGG CCAGTCCCAG 
CCTCTCCCCA GCTCTGCCTT GCCCAGTAGC TTGAGCTCAG TGGATGGACC TGAGGCGGCT 
TCACCAGATA CCAGACACCT GTGGCTGGAG TTCAGCCACC CTGTGGAGAG CCTGGCTCTG 
ACTGTGGAAG AGGTGGTGGA CGTGCGCAGG GTGCTGGTGA AGGCGGAGAT GGAGAGGTTT 
CTGCAGGACA AGGAGCTCTT CAGCAGCCTG AAGAGGGGGA AGATTTGCTG TTGCTGCCGA 
GCCAAGTTTC CCCTGTTTTC CTGGCCACCC ACCTGTCTCT TCTGCAAGAG AGCTGTGTGC 
ACTTCCTGTA GCGTAAAGAT GAAGATGCCT TCCAAGAAGT ATGGACACAT CCCTGTCTAC 
ACACTTGGCT TTGAGAGTCT TCAGAGGGTG CCAACTACCA AAGCCACGCC AACACTGAGG 
AGAGATGCCT TCCAATCCCT GCAGGGACCA AAGTGGCGGA GCGTGGAGGA GGAGTTCCCC 
CACATCTATG CCCACGGCTG TGTCCTAAAG GATGTCTGCA GTGACTGCAC CAGCTTTGTG 
GCTGATGTCG TGTGCTCCAG CCGCAAGAGC GTGGATGTCC TCAATGCCAC TCCACGACGA 
AGCCGTCAGA CCCAGTCCCT CTATATCCCC AACACCAGGA CTCTCAACTT CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_758491.1
CDS	53..2209
Translation	MARAGGGGAAAPERAGGAARPEPWELSLEEVLKVYEQPINEEQAWAVCFQGCRGLRGEPGGVRRIRDTADILLRRDGSVGARLEPEPTTMVVPPASSEAQMVQSLGFAIYRALDWGLDENEERELSPQLERLIDLMANSDCEDSSCGAADEGYVGPEEEEEAEGGPRAVRTFAQAMRLCALRLTDPHGAQAHYQAVCRALFVETLELRAFLARVREAKEMLKKLGEEEPREKPLAELDHLGHTDWARLWVQLMRELRHGVKLKKVQEKEFNPLPTEFQLTPFEMLMQDIRARNYKLRKVMVDGDIPPRVKKDAHELILDFIRSRPPLKQVSERQLRPVPQKQRTLHEKILEEIKQERRLRPVGAQHLGGRGFGSLPCILNACSGDIKSTSCINLSVTDTGSGSQRPRPRVLLKAPTLAEMEEMNTSEEEESPCGEVALKRDRSFSEHDLAQLRSEMASGLQSAAQPPGGTEPPRARAGSMHSWRPSSRDQGFCPVSGQSQPLPSSALPSSLSSVDGPEAASPDTRHLWLEFSHPVESLALTVEEVVDVRRVLVKAEMERFLQDKELFSSLKRGKICCCCRAKFPLFSWPPTCLFCKRAVCTSCSVKMKMPSKKYGHIPVYTLGFESLQRVPTTKATPTLRRDAFQSLQGPKWRSVEEEFPHIYAHGCVLKDVCSDCTSFVADVVCSSRKSVDVLNATPRRSRQTQSLYIPNTRTLNFQ

Target ORF information:

RefSeq Version	NM_172287.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spire type actin nucleation factor 2 (Spire2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172287.2

ORF Insert Sequence:

ATGGCCCGGG CGGGCGGCGG CGGCGCAGCA GCTCCCGAAC GCGCGGGGGG CGCGGCGCGG 
CCCGAGCCGT GGGAGCTGTC CCTGGAGGAA GTGCTGAAGG TGTACGAGCA GCCCATCAAC 
GAGGAGCAGG CCTGGGCGGT GTGCTTCCAG GGCTGCCGCG GGCTGCGGGG CGAGCCGGGC 
GGCGTGCGGC GCATCCGGGA CACGGCAGAC ATCCTCCTGC GTAGGGACGG TTCGGTTGGC 
GCGCGGCTGG AGCCCGAGCC TACAACCATG GTGGTACCAC CAGCCAGCTC AGAAGCCCAG 
ATGGTGCAGT CACTGGGCTT TGCCATTTAC CGTGCGCTGG ACTGGGGGTT AGATGAGAAC 
GAGGAGAGAG AGCTCAGCCC GCAGCTGGAA AGGCTCATCG ACCTCATGGC CAACAGCGAC 
TGCGAAGACA GCAGCTGCGG GGCAGCGGAT GAGGGCTACG TGGGTCCAGA AGAGGAGGAG 
GAGGCTGAGG GCGGCCCCCG AGCTGTGCGC ACCTTTGCCC AGGCTATGCG GCTGTGTGCT 
TTGCGCCTGA CCGACCCCCA CGGCGCGCAG GCCCACTACC AGGCAGTGTG CCGAGCTCTC 
TTTGTGGAGA CACTGGAGCT TCGTGCCTTC CTGGCCCGGG TGAGGGAGGC CAAAGAGATG 
CTGAAGAAAC TGGGGGAGGA GGAGCCACGG GAGAAGCCAC TGGCCGAGCT CGACCACCTG 
GGACACACAG ACTGGGCCCG GCTGTGGGTC CAGCTCATGC GGGAGCTCCG TCACGGGGTA 
AAGCTGAAGA AGGTGCAGGA GAAGGAGTTC AACCCACTGC CCACCGAGTT CCAGCTCACC 
CCCTTCGAGA TGCTGATGCA GGATATCCGT GCCAGGAACT ATAAGCTGCG CAAGGTCATG 
GTCGATGGGG ACATCCCTCC CAGGGTGAAG AAGGACGCAC ATGAACTTAT CCTGGACTTC 
ATTCGTTCTC GGCCTCCACT GAAGCAGGTC TCAGAGAGAC AACTTCGCCC TGTACCACAG 
AAGCAAAGAA CACTGCACGA GAAGATCCTG GAGGAAATCA AGCAGGAGCG GAGGCTTCGT 
CCAGTCGGGG CCCAGCACTT GGGAGGCCGT GGGTTTGGTT CCCTGCCCTG CATCCTCAAT 
GCCTGCTCTG GGGACATCAA GTCCACTTCC TGCATCAACC TGTCTGTCAC CGATACTGGG 
AGCGGGAGTC AGCGCCCACG GCCCCGGGTC CTGCTCAAAG CCCCCACCCT TGCAGAGATG 
GAAGAAATGA ACACGTCTGA GGAAGAAGAG TCCCCATGTG GGGAGGTGGC ACTGAAGCGG 
GACCGCTCTT TTTCGGAACA CGACCTGGCC CAGCTTCGGA GCGAGATGGC ATCTGGCCTT 
CAGTCAGCCG CTCAGCCCCC AGGAGGGACG GAGCCACCTC GGGCCAGAGC AGGTAGCATG 
CACTCCTGGA GGCCCAGCAG CCGAGACCAG GGTTTCTGTC CTGTGAGCGG CCAGTCCCAG 
CCTCTCCCCA GCTCTGCCTT GCCCAGTAGC TTGAGCTCAG TGGATGGACC TGAGGCGGCT 
TCACCAGATA CCAGACACCT GTGGCTGGAG TTCAGCCACC CTGTGGAGAG CCTGGCTCTG 
ACTGTGGAAG AGGTGGTGGA CGTGCGCAGG GTGCTGGTGA AGGCGGAGAT GGAGAGGTTT 
CTGCAGGACA AGGAGCTCTT CAGCAGCCTG AAGAGGGGGA AGATTTGCTG TTGCTGCCGA 
GCCAAGTTTC CCCTGTTTTC CTGGCCACCC ACCTGTCTCT TCTGCAAGAG AGCTGTGTGC 
ACTTCCTGTA GCGTAAAGAT GAAGATGCCT TCCAAGAAGT ATGGACACAT CCCTGTCTAC 
ACACTTGGCT TTGAGAGTCT TCAGAGGGTG CCAACTACCA AAGCCACGCC AACACTGAGG 
AGAGATGCCT TCCAATCCCT GCAGGGACCA AAGTGGCGGA GCGTGGAGGA GGAGTTCCCC 
CACATCTATG CCCACGGCTG TGTCCTAAAG GATGTCTGCA GTGACTGCAC CAGCTTTGTG 
GCTGATGTCG TGTGCTCCAG CCGCAAGAGC GTGGATGTCC TCAATGCCAC TCCACGACGA 
AGCCGTCAGA CCCAGTCCCT CTATATCCCC AACACCAGGA CTCTCAACTT CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.
eLife5(2016 09)
Pylypenko O,Welz T,Tittel J,Kollmar M,Chardon F,Malherbe G,Weiss S,Michel CI,Samol-Wolf A,Grasskamp AT,Hume A,Goud B,Baron B,England P,Titus MA,Schwille P,Weidemann T,Houdusse A,Kerkhoff E

Enhanced fear expression in Spir-1 actin organizer mutant mice.
European journal of cell biology93(5-6)225-37(2014 May-Jun)
Pleiser S,Banchaabouchi MA,Samol-Wolf A,Farley D,Welz T,Wellbourne-Wood J,Gehring I,Linkner J,Faix J,Riemenschneider MJ,Dietrich S,Kerkhoff E

Spir2; a novel QTL on chromosome 4 contributes to susceptibility to pneumococcal infection in mice.
BMC genomics14242(2013 Apr)
Wisby L,Fernandes VE,Neill DR,Kadioglu A,Andrew PW,Denny P

An actin-dependent mechanism for long-range vesicle transport.
Nature cell biology13(12)1431-6(2011 Oct)
Schuh M

Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.
Current biology : CB21(11)955-60(2011 Jun)
Pfender S,Kuznetsov V,Pleiser S,Kerkhoff E,Schuh M