Trim60 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim60
Entrez Gene ID	234329
Full Name	tripartite motif-containing 60
Synonyms	2czf45,Rnf33
General protein information	Preferred Names tripartite motif-containing 60 Names tripartite motif-containing protein 60 ring finger protein 33
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_153097.2	NP_694737.1	tripartite motif-containing protein 60

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRIM61	XP_005642517.1
Mus musculus (house mouse)	Trim60	NP_694737.1
Homo sapiens (human)	TRIM60	NP_689833.1
Pan troglodytes (chimpanzee)	TRIM60	XP_526717.1
	TRIM60	XP_001100317.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of the testis-specific TRIM/RBCC protein RNF33/TRIM60 with the cytoplasmic motor proteins KIF3A and KIF3B.
Huang CJ, Huang CC, Chang CC
Molecular and cellular biochemistry360(1-2)121-31(2012 Jan)

Different modes of regulation of transcription and pre-mRNA processing of the structurally juxtaposed homologs, Rnf33 and Rnf35, in eggs and in pre-implantation embryos.
Choo KB, Chen HH, Liu TY, Chang CP
Nucleic acids research30(22)4836-44(2002 Nov)

Use of a common promoter by two juxtaposed and intronless mouse early embryonic genes, Rnf33 and Rnf35: implications in zygotic gene expression.
Chen HH, Liu TY, Li H, Choo KB
Genomics80(2)140-3(2002 Aug)

In silico mining of EST databases for novel pre-implantation embryo-specific zinc finger protein genes.
Choo KB, Chen HH, Cheng WT, Chang HS, Wang M
Molecular reproduction and development59(3)249-55(2001 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the first demonstration of a TRIM protein, RNF33, interacting with kinesin molecular motors, possibly contributing to kinesin-dependent mobilization along the microtubule in the testis of the mouse
Title: Association of the testis-specific TRIM/RBCC protein RNF33/TRIM60 with the cytoplasmic motor proteins KIF3A and KIF3B.

Regulation of transcription and pre-mRNA processing of Rnf33 in eggs and in pre-implantation embryos
Title: Different modes of regulation of transcription and pre-mRNA processing of the structurally juxtaposed homologs, Rnf33 and Rnf35, in eggs and in pre-implantation embryos.

Rnf33 and Rnf35 mRNAs are apparently transcribed from the same putative promoter and may be products of alternative splicing.
Title: Use of a common promoter by two juxtaposed and intronless mouse early embryonic genes, Rnf33 and Rnf35: implications in zygotic gene expression.

The following Trim60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02274	NM_153097.2 Latest version!	Mus musculus tripartite motif-containing 60 (Trim60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim60 ( NM_153097.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694737.1 Mus musculus tripartite motif-containing 60 (Trim60), mRNA.

Price & Availability↑

CloneID	OMu02274
Clone ID Related Accession (Same CDS sequence)	NM_153097.2
Accession Version	NM_153097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	tripartite motif-containing protein 60
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA561473.1, AK135841.1 and AC164589.6. On Feb 5, 2010 this sequence version replaced NM_153097.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK135841.1, AF290197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCCA CAGCCCTAAA AATCTTGCAG GACAAATGTA TCTGTTACAT TTGTTCGGAT 
TTCATGGAAG ACCCAGTGAC CAGTAGGTGT GGGCACAACT TCTGTTTTGC CTGCCTCCGT 
TTGCTCTGGG ATGATCTACA AGGCAACATC TTCTGTCCCG TCTGTCAAAC CCCCTTTCCA 
CCGAAAAGCT TCAGCAGGAA CTACCAGTTC CGTAACATGA CTGAAACCAT TAGGCTCCTG 
CAAAAGAGAC AGAGCAAGAG GAAGAGGCAG GAAGAACACA CAGTATGTCC GAAGCATGAT 
CAGCCTCTGG TCTTGTTCTG TGTGAGGGAT CGGGATGTCT TATGTACCCA GTGTAGCCTC 
TCTGTCGAAC ACCAGGGCCA TTACACCTGC CCCATTAAGA AAGCCAGCTC TTATCACAGG 
AAAGTCCTAG AGAGCGCCAT TGCAACGTTA AAGTTCGGAG TGAAACAAGT TGAAGAAAAG 
CTAGCTGTGC AGCACAGAAG GGTTCTTGGA CTGAGGGAGG AGGCTCAATA TCAAAAAATA 
GAGATACGTT ATGAAATTGG GCAAATCAAA CTGTTCCTAC AAAGTGAGTA CGAAGCGCAT 
CTAAATGAGA GCCACATGGA GGAGCTGCGG AGTTTCTCAG AACTGAATGG ATATCTTGAG 
ACCCTGTTAG ACCATGTTTC CACAGCAAAA GATCTGTTGA AGGAAGTGGA GGCCATACAT 
GAAAGGTCAG ATGTCACACT CCTGAGAGCC TACCACAAGT TGCAAAACCT TAAATCCCCC 
AAACCGTGGT TATTTAGGAC AAAGCAATAT GGCCTCAGTC TCCCAGCGCA GTATTCTGGC 
TTGAGCAGAA TCATCAAGCA ATTTCAAGCA GATGTGACTT TTGATCGTGA CACAGCACAC 
CCTCAGCTGG TTATTTCTGA GGATAGAAAA TCTGTGTTTT ATAAAGAAGC ATGGCCATGT 
GTGTGTGCTA GCCCTCAGAA ATTTCACCTC TGGCCCGCTC TCCTGGGATG TAAAGGATTC 
GATTCTGGTC GACAGTACTG GGAAGTCAAA GTGGGAGACA AGCCCAGATG GACACTGGGT 
GTGTGTCAAG CCCACTTTTC TGGGGACTGG AGCAATCAGT CCTCAGGTTT CTGGGCAATT 
GGGCGGTATG CTGAGAATAG TTATGTCACC TATGGACCTC TAAGAACGGA GTTTCTGCCA 
GTGGTGCGAC CCAGCAAGGT CGGCATATTT TTGGATTATG AATTGGGTGA GCTCTCCTTT 
TATAATATGA ATGACAGGTC TCTTCTCTAC ACTTTCAGGA ATTCTTTTAC CAGCACCCTT 
TGGCCTTATT TCTATATAGG AACAGATTCT GAATCTCTTG AAATCTTAAC CCACCCCACC 
CCAGACACAG GGTCTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694737.1
CDS	486..1886
Translation	MDSTALKILQDKCICYICSDFMEDPVTSRCGHNFCFACLRLLWDDLQGNIFCPVCQTPFPPKSFSRNYQFRNMTETIRLLQKRQSKRKRQEEHTVCPKHDQPLVLFCVRDRDVLCTQCSLSVEHQGHYTCPIKKASSYHRKVLESAIATLKFGVKQVEEKLAVQHRRVLGLREEAQYQKIEIRYEIGQIKLFLQSEYEAHLNESHMEELRSFSELNGYLETLLDHVSTAKDLLKEVEAIHERSDVTLLRAYHKLQNLKSPKPWLFRTKQYGLSLPAQYSGLSRIIKQFQADVTFDRDTAHPQLVISEDRKSVFYKEAWPCVCASPQKFHLWPALLGCKGFDSGRQYWEVKVGDKPRWTLGVCQAHFSGDWSNQSSGFWAIGRYAENSYVTYGPLRTEFLPVVRPSKVGIFLDYELGELSFYNMNDRSLLYTFRNSFTSTLWPYFYIGTDSESLEILTHPTPDTGSY

Target ORF information:

RefSeq Version	NM_153097.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 60 (Trim60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153097.2

ORF Insert Sequence:

ATGGACTCCA CAGCCCTAAA AATCTTGCAG GACAAATGTA TCTGTTACAT TTGTTCGGAT 
TTCATGGAAG ACCCAGTGAC CAGTAGGTGT GGGCACAACT TCTGTTTTGC CTGCCTCCGT 
TTGCTCTGGG ATGATCTACA AGGCAACATC TTCTGTCCCG TCTGTCAAAC CCCCTTTCCA 
CCGAAAAGCT TCAGCAGGAA CTACCAGTTC CGTAACATGA CTGAAACCAT TAGGCTCCTG 
CAAAAGAGAC AGAGCAAGAG GAAGAGGCAG GAAGAACACA CAGTATGTCC GAAGCATGAT 
CAGCCTCTGG TCTTGTTCTG TGTGAGGGAT CGGGATGTCT TATGTACCCA GTGTAGCCTC 
TCTGTCGAAC ACCAGGGCCA TTACACCTGC CCCATTAAGA AAGCCAGCTC TTATCACAGG 
AAAGTCCTAG AGAGCGCCAT TGCAACGTTA AAGTTCGGAG TGAAACAAGT TGAAGAAAAG 
CTAGCTGTGC AGCACAGAAG GGTTCTTGGA CTGAGGGAGG AGGCTCAATA TCAAAAAATA 
GAGATACGTT ATGAAATTGG GCAAATCAAA CTGTTCCTAC AAAGTGAGTA CGAAGCGCAT 
CTAAATGAGA GCCACATGGA GGAGCTGCGG AGTTTCTCAG AACTGAATGG ATATCTTGAG 
ACCCTGTTAG ACCATGTTTC CACAGCAAAA GATCTGTTGA AGGAAGTGGA GGCCATACAT 
GAAAGGTCAG ATGTCACACT CCTGAGAGCC TACCACAAGT TGCAAAACCT TAAATCCCCC 
AAACCGTGGT TATTTAGGAC AAAGCAATAT GGCCTCAGTC TCCCAGCGCA GTATTCTGGC 
TTGAGCAGAA TCATCAAGCA ATTTCAAGCA GATGTGACTT TTGATCGTGA CACAGCACAC 
CCTCAGCTGG TTATTTCTGA GGATAGAAAA TCTGTGTTTT ATAAAGAAGC ATGGCCATGT 
GTGTGTGCTA GCCCTCAGAA ATTTCACCTC TGGCCCGCTC TCCTGGGATG TAAAGGATTC 
GATTCTGGTC GACAGTACTG GGAAGTCAAA GTGGGAGACA AGCCCAGATG GACACTGGGT 
GTGTGTCAAG CCCACTTTTC TGGGGACTGG AGCAATCAGT CCTCAGGTTT CTGGGCAATT 
GGGCGGTATG CTGAGAATAG TTATGTCACC TATGGACCTC TAAGAACGGA GTTTCTGCCA 
GTGGTGCGAC CCAGCAAGGT CGGCATATTT TTGGATTATG AATTGGGTGA GCTCTCCTTT 
TATAATATGA ATGACAGGTC TCTTCTCTAC ACTTTCAGGA ATTCTTTTAC CAGCACCCTT 
TGGCCTTATT TCTATATAGG AACAGATTCT GAATCTCTTG AAATCTTAAC CCACCCCACC 
CCAGACACAG GGTCTTACTA A