Lim2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lim2
Entrez Gene ID	233187
Full Name	lens intrinsic membrane protein 2
Synonyms	19kDa,4833403J20,MP19,To3
General protein information	Preferred Names lens intrinsic membrane protein 2 Names lens fiber membrane intrinsic protein MP17 MP18 MP20 total opacity 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B3\|7 28.25 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_177693.4	NP_808361.1	lens fiber membrane intrinsic protein
NM_177693.3	NP_808361.1	lens fiber membrane intrinsic protein

Pathways from BioSystems

Homologies

	LIM2	XP_001116349.1
Mus musculus (house mouse)	Lim2	NP_808361.1
Danio rerio (zebrafish)	lim2.5	NP_001013540.1
Homo sapiens (human)	LIM2	NP_085915.2
Pan troglodytes (chimpanzee)	LIM2	XP_001174186.1
Xenopus tropicalis (tropical clawed frog)	lim2	NP_001096249.1
Rattus norvegicus (Norway rat)	Lim2	NP_446223.1
Canis lupus familiaris (dog)	LIM2	XP_003638853.1
Bos taurus (cattle)	LIM2	NP_776527.1

Gene Ontology
Bibliography

Related articles in PubMed

Further analysis of the lens phenotype in Lim2-deficient mice.
Shi Y, De Maria AB, Wang H, Mathias RT, FitzGerald PG, Bassnett S
Investigative ophthalmology & visual science52(10)7332-9(2011 Sep)

Microphakia and congenital cataract formation in a novel Lim2(C51R) mutant mouse.
Puk O, Ahmad N, Wagner S, de Angelis MH, Graw J
Molecular vision171164-71(2011)

The lineage contribution and role of Gbx2 in spinal cord development.
Luu B, Ellisor D, Zervas M
PloS one6(6)e20940(2011)

The stratified syncytium of the vertebrate lens.
Shi Y, Barton K, De Maria A, Petrash JM, Shiels A, Bassnett S
Journal of cell science122(Pt 10)1607-15(2009 May)

The oncoprotein LMO3 interacts with calcium- and integrin-binding protein CIB.
Hui L, Ji C, Hui B, Lv T, Ha X, Yang J, Cai W
Brain research126524-9(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel missense mutation in the Lim2 gene affects lens development in a semidominant manner. Since homozygous mutants develop congenital lens opacities, this mouse line can be used as a model for inherited cataract formation in humans.
Title: Microphakia and congenital cataract formation in a novel Lim2(C51R) mutant mouse.

Data show that the calcium- and integrin-binding protein CIB as an LMO3-binding protein, which binds via the second LIM domain (LIM2) of LMO3.
Title: The oncoprotein LMO3 interacts with calcium- and integrin-binding protein CIB.

The following Lim2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lim2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06659	NM_177693.4 Latest version!	Mus musculus lens intrinsic membrane protein 2 (Lim2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu06659	NM_177693.3	Mus musculus lens intrinsic membrane protein 2 (Lim2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lim2 ( NM_177693.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808361.1 Mus musculus lens intrinsic membrane protein 2 (Lim2), mRNA.

Price & Availability↑

CloneID	OMu06659
Clone ID Related Accession (Same CDS sequence)	NM_177693.4 , NM_177693.3
Accession Version	NM_177693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	lens fiber membrane intrinsic protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK780500.1, AK029354.1 and CX734633.1. On Jun 9, 2018 this sequence version replaced NM_177693.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092386.1, AK029354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCATGGGTGG CGGCCTCTTC TGTGCCTGGG TGGGGACCAT CCTGTTGGTG 
GTAGCCACAG CAACTGACCA CTGGATGCAG TACCGGCTGT CGGGGTCCTT CGCACACCAG 
GGCCTGTGGC GTTACTGCCT GGGCAACAAG TGCTTCCTGC AGACCGAGAG CATCGCATAT 
TGGAATGCCA CCCGGGCTTT CATGATCCTG TCTGCCCTGT GTGCCACCTC GGGCATCATC 
ATGGGTGTCC TAGCCTTTGC GCAGCAATCC ACCTTCACCC GTCTCTCCAG GCCCTTCTCT 
GCCGGCATCA TGTTTTTTGC CTCCACCCTT TTTGTCCTGT TGGCCTTGGC TATTTACACT 
GGAGTCACCG TCAGTTTCCT CGGCCGCCGC TTTGGAGACT GGCGCTTTTC ATGGTCTTAC 
ATCCTGGGCT GGGTGGCCCT GCTCATGACC TTCTTTGCAG GAATTTTCTA CATGTGTGCC 
TACCGGATGC ATGAGTGCCG GCGCCTAGCC ACCCCACGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808361.1
CDS	65..586
Translation	MYSFMGGGLFCAWVGTILLVVATATDHWMQYRLSGSFAHQGLWRYCLGNKCFLQTESIAYWNATRAFMILSALCATSGIIMGVLAFAQQSTFTRLSRPFSAGIMFFASTLFVLLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVALLMTFFAGIFYMCAYRMHECRRLATPR

Target ORF information:

RefSeq Version	NM_177693.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lens intrinsic membrane protein 2 (Lim2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177693.4

ORF Insert Sequence:

ATGTACAGCT TCATGGGTGG CGGCCTCTTC TGTGCCTGGG TGGGGACCAT CCTGTTGGTG 
GTAGCCACAG CAACTGACCA CTGGATGCAG TACCGGCTGT CGGGGTCCTT CGCACACCAG 
GGCCTGTGGC GTTACTGCCT GGGCAACAAG TGCTTCCTGC AGACCGAGAG CATCGCATAT 
TGGAATGCCA CCCGGGCTTT CATGATCCTG TCTGCCCTGT GTGCCACCTC GGGCATCATC 
ATGGGTGTCC TAGCCTTTGC GCAGCAATCC ACCTTCACCC GTCTCTCCAG GCCCTTCTCT 
GCCGGCATCA TGTTTTTTGC CTCCACCCTT TTTGTCCTGT TGGCCTTGGC TATTTACACT 
GGAGTCACCG TCAGTTTCCT CGGCCGCCGC TTTGGAGACT GGCGCTTTTC ATGGTCTTAC 
ATCCTGGGCT GGGTGGCCCT GCTCATGACC TTCTTTGCAG GAATTTTCTA CATGTGTGCC 
TACCGGATGC ATGAGTGCCG GCGCCTAGCC ACCCCACGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lim2 ( NM_177693.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808361.1 Mus musculus lens intrinsic membrane protein 2 (Lim2), mRNA.

Price & Availability↑

CloneID	OMu06659
Clone ID Related Accession (Same CDS sequence)	NM_177693.4 , NM_177693.3
Accession Version	NM_177693.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Mus musculus(house mouse)
Product	lens fiber membrane intrinsic protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK029354.1. On Apr 6, 2007 this sequence version replaced NM_177693.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092386.1, AK029354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164141 [ECO:0000348] ##Evidence-Data-END##

ATGTACAGCT TCATGGGTGG CGGCCTCTTC TGTGCCTGGG TGGGGACCAT CCTGTTGGTG 
GTAGCCACAG CAACTGACCA CTGGATGCAG TACCGGCTGT CGGGGTCCTT CGCACACCAG 
GGCCTGTGGC GTTACTGCCT GGGCAACAAG TGCTTCCTGC AGACCGAGAG CATCGCATAT 
TGGAATGCCA CCCGGGCTTT CATGATCCTG TCTGCCCTGT GTGCCACCTC GGGCATCATC 
ATGGGTGTCC TAGCCTTTGC GCAGCAATCC ACCTTCACCC GTCTCTCCAG GCCCTTCTCT 
GCCGGCATCA TGTTTTTTGC CTCCACCCTT TTTGTCCTGT TGGCCTTGGC TATTTACACT 
GGAGTCACCG TCAGTTTCCT CGGCCGCCGC TTTGGAGACT GGCGCTTTTC ATGGTCTTAC 
ATCCTGGGCT GGGTGGCCCT GCTCATGACC TTCTTTGCAG GAATTTTCTA CATGTGTGCC 
TACCGGATGC ATGAGTGCCG GCGCCTAGCC ACCCCACGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808361.1
CDS	63..584
Translation	MYSFMGGGLFCAWVGTILLVVATATDHWMQYRLSGSFAHQGLWRYCLGNKCFLQTESIAYWNATRAFMILSALCATSGIIMGVLAFAQQSTFTRLSRPFSAGIMFFASTLFVLLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVALLMTFFAGIFYMCAYRMHECRRLATPR

Target ORF information:

RefSeq Version	NM_177693.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lens intrinsic membrane protein 2 (Lim2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177693.3

ORF Insert Sequence:

ATGTACAGCT TCATGGGTGG CGGCCTCTTC TGTGCCTGGG TGGGGACCAT CCTGTTGGTG 
GTAGCCACAG CAACTGACCA CTGGATGCAG TACCGGCTGT CGGGGTCCTT CGCACACCAG 
GGCCTGTGGC GTTACTGCCT GGGCAACAAG TGCTTCCTGC AGACCGAGAG CATCGCATAT 
TGGAATGCCA CCCGGGCTTT CATGATCCTG TCTGCCCTGT GTGCCACCTC GGGCATCATC 
ATGGGTGTCC TAGCCTTTGC GCAGCAATCC ACCTTCACCC GTCTCTCCAG GCCCTTCTCT 
GCCGGCATCA TGTTTTTTGC CTCCACCCTT TTTGTCCTGT TGGCCTTGGC TATTTACACT 
GGAGTCACCG TCAGTTTCCT CGGCCGCCGC TTTGGAGACT GGCGCTTTTC ATGGTCTTAC 
ATCCTGGGCT GGGTGGCCCT GCTCATGACC TTCTTTGCAG GAATTTTCTA CATGTGTGCC 
TACCGGATGC ATGAGTGCCG GCGCCTAGCC ACCCCACGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Further analysis of the lens phenotype in Lim2-deficient mice.
Investigative ophthalmology & visual science52(10)7332-9(2011 Sep)
Shi Y,De Maria AB,Wang H,Mathias RT,FitzGerald PG,Bassnett S

Microphakia and congenital cataract formation in a novel Lim2(C51R) mutant mouse.
Molecular vision171164-71(2011)
Puk O,Ahmad N,Wagner S,de Angelis MH,Graw J

The lineage contribution and role of Gbx2 in spinal cord development.
PloS one6(6)e20940(2011)
Luu B,Ellisor D,Zervas M

The stratified syncytium of the vertebrate lens.
Journal of cell science122(Pt 10)1607-15(2009 May)
Shi Y,Barton K,De Maria A,Petrash JM,Shiels A,Bassnett S

The oncoprotein LMO3 interacts with calcium- and integrin-binding protein CIB.
Brain research126524-9(2009 Apr)
Hui L,Ji C,Hui B,Lv T,Ha X,Yang J,Cai W