Otop1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Otop1
Entrez Gene ID	21906
Full Name	otopetrin 1
Synonyms	A530025J20Rik,Otp1,tlt
General protein information	Preferred Names otopetrin 1 Names otopetrin-1 tilted
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 B3\|5 20.35 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_172709.3	NP_766297.2	proton channel OTOP1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	OTOP1	XP_545943.3
Mus musculus (house mouse)	Otop1	NP_766297.2
Rattus norvegicus (Norway rat)	Otop1	NP_852098.1
Gallus gallus (chicken)	OTOP1	XP_420790.3
Pan troglodytes (chimpanzee)	OTOP1	XP_526509.2
Bos taurus (cattle)	OTOP1	NP_001193713.1
Danio rerio (zebrafish)	otop1	NP_942098.1
Homo sapiens (human)	OTOP1	NP_819056.1
	OTOP1	XP_001097009.1
Xenopus tropicalis (tropical clawed frog)	otop1	XP_002939096.2

Gene Ontology
Bibliography

Related articles in PubMed

Otolith dysfunction alters exploratory movement in mice.
Blankenship PA, Cherep LA, Donaldson TN, Brockman SN, Trainer AD, Yoder RM, Wallace DG
Behavioural brain research325(Pt A)1-11(2017 05)

Spatiotemporal differences in otoconial gene expression.
Xu Y, Zhang Y, Lundberg YW
Genesis (New York, N.Y. : 2000)54(12)613-625(2016 Dec)

Otopetrin 1 protects mice from obesity-associated metabolic dysfunction through attenuating adipose tissue inflammation.
Wang GX, Cho KW, Uhm M, Hu CR, Li S, Cozacov Z, Xu AE, Cheng JX, Saltiel AR, Lumeng CN, Lin JD
Diabetes63(4)1340-52(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Otop1 defines a unique target of cytokine signaling that attenuates obesity-induced adipose tissue inflammation and plays an adaptive role in maintaining metabolic homeostasis in obesity.
Title: Otopetrin 1 protects mice from obesity-associated metabolic dysfunction through attenuating adipose tissue inflammation.

Data show that tlt and mlh mutations uncouple Otopetrin 1 from inhibition of P2Y receptor function.
Title: Missense mutations in Otopetrin 1 affect subcellular localization and inhibition of purinergic signaling in vestibular supporting cells.

OTOP1 is expressed at the apex of supporting cells. It increases cytosolic Ca in response to purinergic agonists by blocking mobilization of Ca from intracellular stores in an extracellular Ca-dependent manner & by mediating extracellular Ca influx.
Title: Regulation of cellular calcium in vestibular supporting cells by otopetrin 1.

a non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice is caused by mutations in otopetrin 1
Title: Non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice caused by mutations in otopetrin 1.

The following Otop1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Otop1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15027	NM_172709.3 Latest version!	Mus musculus otopetrin 1 (Otop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Otop1 ( NM_172709.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766297.2 Mus musculus otopetrin 1 (Otop1), mRNA.

Price & Availability↑

CloneID	OMu15027
Clone ID Related Accession (Same CDS sequence)	NM_172709.3
Accession Version	NM_172709.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	proton channel OTOP1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF548337.1, AK040792.1 and EH101457.1. On Apr 13, 2007 this sequence version replaced NM_172709.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF548337.1, AK040792.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGGG GCCCGGGCGC GCCCTCTTCT CCCGCAGCAT CCTCAGGCTC CTCGCGTGCC 
GCGCCGTCGG GGATCGCGGC ATGCCCTCTG TCCCCACCTC CCCTAGCCCG AGGGTCTCCA 
CAAGCTTCCG GTCCCCGGCG CGGCGCCAGC GTCCCGCAGA AGCTGGCGGA GACTCTGAGC 
AGCCAGTATG GACTGAACGT GTTCGTGGCG GGGCTGCTGT TCCTGCTGGC CTGGGCGGTG 
CACGCGACGG GTGTGGGCAA GAGCGACCTG CTGTGCGTTC TAACCGCGCT TATGCTGCTG 
CAGCTGCTCT GGATGCTGTG GTACGTGGGT CGCAGCTACA TGCAGCGCCG CCTCATCCGC 
CCCAAGGACA CGCACGCGGG TGCGCGCTGG CTTCGCGGAA GCATCACGTT GTTCGCGTTT 
ATCACTGTCG TCCTGGGATG CTTGAAAGTC GCATACTTCA TTGGATTCTC GGAGTGCTTG 
TCAGCCACCG AGGGAGTTTT CCCAGTCACC CATGCAGTGC ATACCCTATT GCAGGTGTAT 
TTCCTCTGGG GCCATGCTAA GGATATCATC ATGTCTTTCA AAACACTGGA AAGGTTTGGG 
GTGATCCATT CAGTGTTCAC GAACCTCCTA CTGTGGGCCA ACAGCGTCCT GAATGAATCA 
AAGCACCAGC TGAATGAGCA CAAGGAACGG CTGATCACTC TGGGCTTTGG CAACATCACC 
ATCGTTTTGG ATGACCACAC ACCACAGTGT AACTGCACAC CACCCGCCCT CTGCTCTGCC 
CTCTCCCATG GGATTTACTA TCTGTACCCC TTCAACATTG AGTACCAGAT CCTGGCCTCG 
ACCATGCTCT ACGTGCTGTG GAAGAACATC GGGCGCAGAG TGGACAGCTC CCAGCACCAG 
AAGATGCAGT GCAGATTCGA CGGGGTCCTA GTGGGCTCCG TGCTGGGCTT GACAGTGCTG 
GCTGCCACCA TCGCCGTGGT TGTGGTGTAC ATGATCCACA TCGGGCGCTC CAAATCCAAG 
AGCGAGTCGG CTCTCATCAT GTTCTATTTG TACGCTATCA CGGTGCTGCT GCTTATGGGG 
GCCGCAGGGC TAGTCGGAAG CTGGATTTAC AGGGTGGATG AGAAGTCTCT GGATGAGTCA 
AAGAACCCAG CGCGCAAGCT GGATGTTGAC CTGTTGGTGG CCACCGGCTC CGGCTCCTGG 
CTCCTCTCCT GGGGCTCCAT TCTGGCCATC GCCTGTGCTG AGACTCGCCC ACCGTACACC 
TGGTACAACC TGCCCTACTC GGTCCTGGTG ATCGTGGAGA AGTATGTCCA AAACATTTTC 
ATCATCGAGT CCGTGCACCT CGAGCCTGAG GGGGTCCCGG AGGATGTGCG CACTCTGCGT 
GTGGTCACCG TCTGCAGCAG CGAGGCTGCC GCACTGGCTG CATCCACTCT CGGGAGCCAG 
GGGATGGCCC AGGATGGGTC ACCTGCTGTC AATGGAAATC TGTGTCTGCA GCAGAGGTGT 
GGGAAAGAGG ACCAGGAGTC TGGCTGGGAA GGAGCTACGG GGACAACCCG ATGTCTGGAC 
TTCCTTCAGG GCGGCATGAA GAGGAGGCTT CTCAGAAACA TCACGGCCTT TCTGTTTCTT 
TGCAACATCT CGCTTTGGAT TCCCCCTGCC TTTGGCTGCC GTCCCGAGTA TGACAACGGA 
TTGGAGGAAA TTGTCTTTGG CTTTGAACCC TGGATAATTG TGGTCAACCT GGCCATGCCC 
TTTTCCATTT TCTACCGGAT GCACGCAGCT GCTGCCCTCT TTGAGGTCTA TTGTAAGATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766297.2
CDS	25..1827
Translation	MPGGPGAPSSPAASSGSSRAAPSGIAACPLSPPPLARGSPQASGPRRGASVPQKLAETLSSQYGLNVFVAGLLFLLAWAVHATGVGKSDLLCVLTALMLLQLLWMLWYVGRSYMQRRLIRPKDTHAGARWLRGSITLFAFITVVLGCLKVAYFIGFSECLSATEGVFPVTHAVHTLLQVYFLWGHAKDIIMSFKTLERFGVIHSVFTNLLLWANSVLNESKHQLNEHKERLITLGFGNITIVLDDHTPQCNCTPPALCSALSHGIYYLYPFNIEYQILASTMLYVLWKNIGRRVDSSQHQKMQCRFDGVLVGSVLGLTVLAATIAVVVVYMIHIGRSKSKSESALIMFYLYAITVLLLMGAAGLVGSWIYRVDEKSLDESKNPARKLDVDLLVATGSGSWLLSWGSILAIACAETRPPYTWYNLPYSVLVIVEKYVQNIFIIESVHLEPEGVPEDVRTLRVVTVCSSEAAALAASTLGSQGMAQDGSPAVNGNLCLQQRCGKEDQESGWEGATGTTRCLDFLQGGMKRRLLRNITAFLFLCNISLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHAAAALFEVYCKI

Target ORF information:

RefSeq Version	NM_172709.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus otopetrin 1 (Otop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172709.3

ORF Insert Sequence:

ATGCCTGGGG GCCCGGGCGC GCCCTCTTCT CCCGCAGCAT CCTCAGGCTC CTCGCGTGCC 
GCGCCGTCGG GGATCGCGGC ATGCCCTCTG TCCCCACCTC CCCTAGCCCG AGGGTCTCCA 
CAAGCTTCCG GTCCCCGGCG CGGCGCCAGC GTCCCGCAGA AGCTGGCGGA GACTCTGAGC 
AGCCAGTATG GACTGAACGT GTTCGTGGCG GGGCTGCTGT TCCTGCTGGC CTGGGCGGTG 
CACGCGACGG GTGTGGGCAA GAGCGACCTG CTGTGCGTTC TAACCGCGCT TATGCTGCTG 
CAGCTGCTCT GGATGCTGTG GTACGTGGGT CGCAGCTACA TGCAGCGCCG CCTCATCCGC 
CCCAAGGACA CGCACGCGGG TGCGCGCTGG CTTCGCGGAA GCATCACGTT GTTCGCGTTT 
ATCACTGTCG TCCTGGGATG CTTGAAAGTC GCATACTTCA TTGGATTCTC GGAGTGCTTG 
TCAGCCACCG AGGGAGTTTT CCCAGTCACC CATGCAGTGC ATACCCTATT GCAGGTGTAT 
TTCCTCTGGG GCCATGCTAA GGATATCATC ATGTCTTTCA AAACACTGGA AAGGTTTGGG 
GTGATCCATT CAGTGTTCAC GAACCTCCTA CTGTGGGCCA ACAGCGTCCT GAATGAATCA 
AAGCACCAGC TGAATGAGCA CAAGGAACGG CTGATCACTC TGGGCTTTGG CAACATCACC 
ATCGTTTTGG ATGACCACAC ACCACAGTGT AACTGCACAC CACCCGCCCT CTGCTCTGCC 
CTCTCCCATG GGATTTACTA TCTGTACCCC TTCAACATTG AGTACCAGAT CCTGGCCTCG 
ACCATGCTCT ACGTGCTGTG GAAGAACATC GGGCGCAGAG TGGACAGCTC CCAGCACCAG 
AAGATGCAGT GCAGATTCGA CGGGGTCCTA GTGGGCTCCG TGCTGGGCTT GACAGTGCTG 
GCTGCCACCA TCGCCGTGGT TGTGGTGTAC ATGATCCACA TCGGGCGCTC CAAATCCAAG 
AGCGAGTCGG CTCTCATCAT GTTCTATTTG TACGCTATCA CGGTGCTGCT GCTTATGGGG 
GCCGCAGGGC TAGTCGGAAG CTGGATTTAC AGGGTGGATG AGAAGTCTCT GGATGAGTCA 
AAGAACCCAG CGCGCAAGCT GGATGTTGAC CTGTTGGTGG CCACCGGCTC CGGCTCCTGG 
CTCCTCTCCT GGGGCTCCAT TCTGGCCATC GCCTGTGCTG AGACTCGCCC ACCGTACACC 
TGGTACAACC TGCCCTACTC GGTCCTGGTG ATCGTGGAGA AGTATGTCCA AAACATTTTC 
ATCATCGAGT CCGTGCACCT CGAGCCTGAG GGGGTCCCGG AGGATGTGCG CACTCTGCGT 
GTGGTCACCG TCTGCAGCAG CGAGGCTGCC GCACTGGCTG CATCCACTCT CGGGAGCCAG 
GGGATGGCCC AGGATGGGTC ACCTGCTGTC AATGGAAATC TGTGTCTGCA GCAGAGGTGT 
GGGAAAGAGG ACCAGGAGTC TGGCTGGGAA GGAGCTACGG GGACAACCCG ATGTCTGGAC 
TTCCTTCAGG GCGGCATGAA GAGGAGGCTT CTCAGAAACA TCACGGCCTT TCTGTTTCTT 
TGCAACATCT CGCTTTGGAT TCCCCCTGCC TTTGGCTGCC GTCCCGAGTA TGACAACGGA 
TTGGAGGAAA TTGTCTTTGG CTTTGAACCC TGGATAATTG TGGTCAACCT GGCCATGCCC 
TTTTCCATTT TCTACCGGAT GCACGCAGCT GCTGCCCTCT TTGAGGTCTA TTGTAAGATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Otolith dysfunction alters exploratory movement in mice.
Behavioural brain research325(Pt A)1-11(2017 05)
Blankenship PA,Cherep LA,Donaldson TN,Brockman SN,Trainer AD,Yoder RM,Wallace DG

Spatiotemporal differences in otoconial gene expression.
Genesis (New York, N.Y. : 2000)54(12)613-625(2016 Dec)
Xu Y,Zhang Y,Lundberg YW

Otopetrin 1 protects mice from obesity-associated metabolic dysfunction through attenuating adipose tissue inflammation.
Diabetes63(4)1340-52(2014 Apr)
Wang GX,Cho KW,Uhm M,Hu CR,Li S,Cozacov Z,Xu AE,Cheng JX,Saltiel AR,Lumeng CN,Lin JD