Tjp2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017318137.1	XP_017173626.1	tight junction protein ZO-2 isoform X6
XM_006526909.1	XP_006526972.1	tight junction protein ZO-2 isoform X5
NM_001360392.1	NP_001347321.1	tight junction protein ZO-2 isoform 4
NM_011597.4	NP_035727.2	tight junction protein ZO-2 isoform 2
XM_011247219.2	XP_011245521.1	tight junction protein ZO-2 isoform X4
XM_030250861.1	XP_030106721.1	tight junction protein ZO-2 isoform X5
NM_011597.5	NP_035727.2	tight junction protein ZO-2 isoform 2
XM_006526905.2	XP_006526968.1	tight junction protein ZO-2 isoform X2
NM_001360391.1	NP_001347320.1	tight junction protein ZO-2 isoform 3
XM_006526904.4	XP_006526967.1	tight junction protein ZO-2 isoform X1
XM_006526909.2	XP_006526972.1	tight junction protein ZO-2 isoform X4
NM_001376368.1	NP_001363297.1	tight junction protein ZO-2 isoform 2
NM_001198985.1	NP_001185914.1	tight junction protein ZO-2 isoform 1
XM_006526904.3	XP_006526967.1	tight junction protein ZO-2 isoform X1
XM_017318136.1	XP_017173625.1	tight junction protein ZO-2 isoform X6
NM_001198985.2	NP_001185914.1	tight junction protein ZO-2 isoform 1
XM_006526907.2	XP_006526970.1	tight junction protein ZO-2 isoform X3
XM_006526908.3	XP_006526971.1	tight junction protein ZO-2 isoform X4
XM_006526907.3	XP_006526970.1	tight junction protein ZO-2 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15700	NM_001376368.1 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu55411	XM_006526904.4 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu55414	XM_006526907.3 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu55415	XM_006526909.2 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87540	XM_030250861.1 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu15570	NM_001198985.2 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu15700	NM_011597.5 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu55412	NM_001360391.1 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu79931	NM_001360392.1 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu15700	XM_011247219.2 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu15700	XM_006526908.3 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu55412	XM_006526905.2 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu79931	XM_017318137.1 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu79931	XM_017318136.1 Latest version!	Mus musculus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu15700	NM_011597.4	Mus musculus tight junction protein 2 (Tjp2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu55415	XM_006526909.1	Mus musculus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu55411	XM_006526904.3	Mus musculus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu15570	NM_001198985.1	Mus musculus tight junction protein 2 (Tjp2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu55414	XM_006526907.2	Mus musculus tight junction protein 2 (Tjp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

Tjp2 ( NM_001376368.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001363297.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu15700
Clone ID Related Accession (Same CDS sequence)	NM_011597.4 , XM_011247219.2 , NM_011597.5 , NM_001376368.1 , XM_006526908.3
Accession Version	NM_001376368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-13
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149224.7 and AC107685.7. On Nov 14, 2019 this sequence version replaced XM_006526908.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR6116558.172167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363297.1
CDS	307..3810
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_001376368.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376368.1

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526904.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526967.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55411
Clone ID Related Accession (Same CDS sequence)	XM_006526904.4 , XM_006526904.3
Accession Version	XM_006526904.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526904.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGA ACATAAGGAA ATCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT 
AGCAGAGACC AACTTAGGGA TGCTAGCCCG CCCCCAGCCT TCAAGCCAGA GCCGCCCAAG 
GCAAGAAGCC AGAACAGAGA AGACTCCTTC GACTACTCCA AGTCAAACCT CCCCGCCACA 
GCCGGCAGTG AAATCCCGGG GGGATCCACC AAAGGCTATC CTCCCCCTAT TGCAGCGAAG 
CCTGCCTTTG GGCGGCCCAT CCTGAAGCCT TCCACTCCAG TCCCTATGCC TGAGAGCGAG 
GAGGTTGGAG AGAGCACCGA GGAGCAGGAA GATGCTCCCA GATCAGTCCT GGGCAGAGTG 
AAAATATTCG AGAAGATGGA CCACAAGGCA AAATTACAGA GGATGCAGGA GCTCCAGGAG 
GCCCAGAATG CGAGGATCGA AATAGCTCAG AAGCACCCTG ACATCTATGC GGTTCCAATC 
AAGGCCCCCA AGCCAGATGC TGGCCTCCCC CCGCACATGA GTTCTAGACC CCCAGAGCCC 
CAGAAAGCTC CTTCCAGACT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGACCCCGAG 
GAGGAGGAGT ACCGCCAGCA GTTGGCAGCA CACTCGAAGC GTGGTTACTA CAGCCAGCCC 
TCCCGGTACC GAGACACGGA GTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526967.1
CDS	197..3823
Translation	MTRGRRKGKEEKMPVFRRSTNSLSSGPGKKQALRTTGKGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526904.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526904.4

ORF Insert Sequence:

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGA ACATAAGGAA ATCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT 
AGCAGAGACC AACTTAGGGA TGCTAGCCCG CCCCCAGCCT TCAAGCCAGA GCCGCCCAAG 
GCAAGAAGCC AGAACAGAGA AGACTCCTTC GACTACTCCA AGTCAAACCT CCCCGCCACA 
GCCGGCAGTG AAATCCCGGG GGGATCCACC AAAGGCTATC CTCCCCCTAT TGCAGCGAAG 
CCTGCCTTTG GGCGGCCCAT CCTGAAGCCT TCCACTCCAG TCCCTATGCC TGAGAGCGAG 
GAGGTTGGAG AGAGCACCGA GGAGCAGGAA GATGCTCCCA GATCAGTCCT GGGCAGAGTG 
AAAATATTCG AGAAGATGGA CCACAAGGCA AAATTACAGA GGATGCAGGA GCTCCAGGAG 
GCCCAGAATG CGAGGATCGA AATAGCTCAG AAGCACCCTG ACATCTATGC GGTTCCAATC 
AAGGCCCCCA AGCCAGATGC TGGCCTCCCC CCGCACATGA GTTCTAGACC CCCAGAGCCC 
CAGAAAGCTC CTTCCAGACT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGACCCCGAG 
GAGGAGGAGT ACCGCCAGCA GTTGGCAGCA CACTCGAAGC GTGGTTACTA CAGCCAGCCC 
TCCCGGTACC GAGACACGGA GTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526907.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526970.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.

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CloneID	OMu55414
Clone ID Related Accession (Same CDS sequence)	XM_006526907.2 , XM_006526907.3
Accession Version	XM_006526907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGG CAAGAAGCCA GAACAGAGAA GACTCCTTCG ACTACTCCAA GTCAAACCTC 
CCCGCCACAG CCGGCAGTGA AATCCCGGGG GGATCCACCA AAGGCTATCC TCCCCCTATT 
GCAGCGAAGC CTGCCTTTGG GCGGCCCATC CTGAAGCCTT CCACTCCAGT CCCTATGCCT 
GAGAGCGAGG AGGTTGGAGA GAGCACCGAG GAGCAGGAAG ATGCTCCCAG ATCAGTCCTG 
GGCAGAGTGA AAATATTCGA GAAGATGGAC CACAAGGCAA AATTACAGAG GATGCAGGAG 
CTCCAGGAGG CCCAGAATGC GAGGATCGAA ATAGCTCAGA AGCACCCTGA CATCTATGCG 
GTTCCAATCA AGGCCCCCAA GCCAGATGCT GGCCTCCCCC CGCACATGAG TTCTAGACCC 
CCAGAGCCCC AGAAAGCTCC TTCCAGACTT TACCAGGACA CCAGAGGAAG CTACGGCAGT 
GACCCCGAGG AGGAGGAGTA CCGCCAGCAG TTGGCAGCAC ACTCGAAGCG TGGTTACTAC 
AGCCAGCCCT CCCGGTACCG AGACACGGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526970.1
CDS	14..3529
Translation	MTRGRRKGKEEKMPVFRRSTNSLSSGPGKKQALRTTGKGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526907.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526907.3

ORF Insert Sequence:

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGG CAAGAAGCCA GAACAGAGAA GACTCCTTCG ACTACTCCAA GTCAAACCTC 
CCCGCCACAG CCGGCAGTGA AATCCCGGGG GGATCCACCA AAGGCTATCC TCCCCCTATT 
GCAGCGAAGC CTGCCTTTGG GCGGCCCATC CTGAAGCCTT CCACTCCAGT CCCTATGCCT 
GAGAGCGAGG AGGTTGGAGA GAGCACCGAG GAGCAGGAAG ATGCTCCCAG ATCAGTCCTG 
GGCAGAGTGA AAATATTCGA GAAGATGGAC CACAAGGCAA AATTACAGAG GATGCAGGAG 
CTCCAGGAGG CCCAGAATGC GAGGATCGAA ATAGCTCAGA AGCACCCTGA CATCTATGCG 
GTTCCAATCA AGGCCCCCAA GCCAGATGCT GGCCTCCCCC CGCACATGAG TTCTAGACCC 
CCAGAGCCCC AGAAAGCTCC TTCCAGACTT TACCAGGACA CCAGAGGAAG CTACGGCAGT 
GACCCCGAGG AGGAGGAGTA CCGCCAGCAG TTGGCAGCAC ACTCGAAGCG TGGTTACTAC 
AGCCAGCCCT CCCGGTACCG AGACACGGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526909.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526972.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.

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CloneID	OMu55415
Clone ID Related Accession (Same CDS sequence)	XM_006526909.1 , XM_006526909.2
Accession Version	XM_006526909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGGCA AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC 
TACTCCAAGT CAAACCTCCC CGCCACAGCC GGCAGTGAAA TCCCGGGGGG ATCCACCAAA 
GGCTATCCTC CCCCTATTGC AGCGAAGCCT GCCTTTGGGC GGCCCATCCT GAAGCCTTCC 
ACTCCAGTCC CTATGCCTGA GAGCGAGGAG GTTGGAGAGA GCACCGAGGA GCAGGAAGAT 
GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA ATATTCGAGA AGATGGACCA CAAGGCAAAA 
TTACAGAGGA TGCAGGAGCT CCAGGAGGCC CAGAATGCGA GGATCGAAAT AGCTCAGAAG 
CACCCTGACA TCTATGCGGT TCCAATCAAG GCCCCCAAGC CAGATGCTGG CCTCCCCCCG 
CACATGAGTT CTAGACCCCC AGAGCCCCAG AAAGCTCCTT CCAGACTTTA CCAGGACACC 
AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG GAGGAGTACC GCCAGCAGTT GGCAGCACAC 
TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526972.1
CDS	3912..7385
Translation	MRASQVLQRMWIQAVKKLERWKGHGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526909.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526909.2

ORF Insert Sequence:

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGGCA AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC 
TACTCCAAGT CAAACCTCCC CGCCACAGCC GGCAGTGAAA TCCCGGGGGG ATCCACCAAA 
GGCTATCCTC CCCCTATTGC AGCGAAGCCT GCCTTTGGGC GGCCCATCCT GAAGCCTTCC 
ACTCCAGTCC CTATGCCTGA GAGCGAGGAG GTTGGAGAGA GCACCGAGGA GCAGGAAGAT 
GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA ATATTCGAGA AGATGGACCA CAAGGCAAAA 
TTACAGAGGA TGCAGGAGCT CCAGGAGGCC CAGAATGCGA GGATCGAAAT AGCTCAGAAG 
CACCCTGACA TCTATGCGGT TCCAATCAAG GCCCCCAAGC CAGATGCTGG CCTCCCCCCG 
CACATGAGTT CTAGACCCCC AGAGCCCCAG AAAGCTCCTT CCAGACTTTA CCAGGACACC 
AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG GAGGAGTACC GCCAGCAGTT GGCAGCACAC 
TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_030250861.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106721.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu87540
Clone ID Related Accession (Same CDS sequence)	XM_030250861.1
Accession Version	XM_030250861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CCGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGTCCAGGCA TGGAGGAGGT GATATGGGAG CAGTACACCG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATTGC CGTGTCGGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATCTC AGATGTGCTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AAGATGTGCT CCATTCGTTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATAGCAGCCA TCGTGGTCAA GAGACCCCGG 
AAGGTTCAGG TGGCCCCACT GCAAGGCAGC CCCCCTCTCA GTCATGACGA CCGAGGTTTT 
GAAGTGATTG AAGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGTCGG 
CACAGCAGCC ACGACATGCT CAGCCACAGC TGGGAGGGCA ATAGGGAGAG GGGGCGACCC 
CATCAGCGCA CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCAGGAGCCT GGAACGGGGC 
CTGGACCAGG AAGACTATGG GCGCAGCCGT GAGCGCAGCC GTGGCCGGAG CCTGGAGCGC 
GGGCTGGACC GCGACTTTGT GTCCAGGGAC CACAGCCGTG GCCGTAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC GAAGCTTATG AACCCGACTA CGGAGGCGGC 
TACAGCCCAT CATATGACCG TAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGTTCCCG AAGCCCCAGC CCCGAGTCTA GGTCACGGCA TGAACACAAG 
GGCCAACATG ATCCCGACAG GCCCATCGGG GTCCTTCTAA CCAAAAGCAA AGCAAATGAA 
GAGTATGGGC TCCGGCTGGG GAGTCAGATC TTCATCAAAG AAATGACCAG AACAGGACTG 
GCCACCAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA CGGCACTGTT 
ACTGAGAACA TGTCTCTAAC GGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTTGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCAGC CCTAAACGAC 
AGCGACTCTG AAGTGGAGGA TATCTCGGAA ATAGAGTCCA ACCGGTCTTT CTCTCCAGAG 
GAGAGACGCC AGCAGTATTC TGATCAGGAT TACCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCGAGCT CAAGAGAGGA GACCTCAGGC AGACTATCCA GGATGGGTGC CACTCCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCGGGGGTCA CAGAGGCCAG CAGGGAGCCC 
AGGTACCAGG AAGAAGGCCC AGTTCCTCAA CCCAGAACAG CTCCAAGAGT TTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAG GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAACGACG TTGGGATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCCGC AGAGCAGGAG GGCCTGCAAG AAGGAGACCA GATTCTGAAG 
GTGAACACAC AGGATTTCAG AGGGCTAGTC CGGGAAGATG CGGTCCTCTA CCTGCTAGAA 
ATCCCCAAAG GTGAAACTGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTACAGAGAT 
ATCCTGGCTT GTGGCAGGGG AGATTCGTTT TTCATAAGAA GCCACTTTGA ATGTGAGAAG 
GAAACTCCCC AGAGCCTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AGCTGGGCCA CTGGCTGGCC GTGAGGATCG GGAATGAGCT GGAGAAAGGC 
TTGATCCCTA ACAAAAGCAG AGCCGAGCAA ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AACGCCGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCAG CGGCGGCGTC 
AAGAAGAACC TCCGCAAGAG CCGGGAAGAC CTGGCGGCTG CTGTATCGGT TAGCACCAAG 
TTCCCTGCCT ACGAGAAGGT TCTGCTTCGG GAAGCTGGCT TCAAGAGACC CGTGGTTCTG 
TTTGGCCCCA TAGCAGATAT AGCAATGGAA AGGTTGGCAA CTGAGTTACC AGACCTGTTT 
CAAACTGCAA AAACAGAACC CAAAGATGCC GGATCTGAGA AATCCAGTGG AGTGGTTCGG 
TTGAATACCG TGAGGCAAAT TATTGAGCAG GACAAGCACG CCCTGCTCGA CGTTACCCCC 
AAAGCTGTGG ACCTGCTCAA CTACACTCAG TGGTTCCCTA TCGTGATTTT CTTCAACCCG 
GATTCCAGAC AAGGTGTTAA AACCATAAGG CAGAGGTTGA GTCCAACATC CAATAAAAGT 
TCTCGCAAGT TATTTGATCA AGCCAACAAG CTCAAAAAGA CCTGTTCTCA TCTTTTTACA 
GCCACAATCA ACGTGAATTC AGCCAACGAT GGCTGGTTTG GCAGCTTGAA GGACAGCATT 
CAGCAGCAGC AAAACGAAGC AGTCTGGGTC TCTGAAGGAA AGATGGAAGG GATGGATGAT 
GATGCTGAAG ACCGCATGTC CTACTTAACC GCCATGGGCG CGGACTATCT GAGTTGTGAC 
AGCCGTCTCA TCAGTGACTT TGAAGACACC GACGGCGAGG GAGGCGCCTA CACTGACAAT 
GAGCTAGAGG AGCCGGCGGA GGAGCCGCTG GTGTCTTCCA TCACCCGCTC CTCGGAGCCG 
GTGCAGCACG AGGAGGCAAG AAGCCAGAAC AGAGAAGACT CCTTCGACTA CTCCAAGTCA 
AACCTCCCCG CCACAGCCGG CAGTGAAATC CCGGGGGGAT CCACCAAAGG CTATCCTCCC 
CCTATTGCAG CGAAGCCTGC CTTTGGGCGG CCCATCCTGA AGCCTTCCAC TCCAGTCCCT 
ATGCCTGAGA GCGAGGAGGT TGGAGAGAGC ACCGAGGAGC AGGAAGATGC TCCCAGATCA 
GTCCTGGGCA GAGTGAAAAT ATTCGAGAAG ATGGACCACA AGGCAAAATT ACAGAGGATG 
CAGGAGCTCC AGGAGGCCCA GAATGCGAGG ATCGAAATAG CTCAGAAGCA CCCTGACATC 
TATGCGGTTC CAATCAAGGC CCCCAAGCCA GATGCTGGCC TCCCCCCGCA CATGAGTTCT 
AGACCCCCAG AGCCCCAGAA AGCTCCTTCC AGACTTTACC AGGACACCAG AGGAAGCTAC 
GGCAGTGACC CCGAGGAGGA GGAGTACCGC CAGCAGTTGG CAGCACACTC GAAGCGTGGT 
TACTACAGCC AGCCCTCCCG GTACCGAGAC ACGGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106721.1
CDS	315..3776
Translation	MPVRGDRGFPPRRELSGWQRGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_030250861.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250861.1

ORF Insert Sequence:

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CCGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGTCCAGGCA TGGAGGAGGT GATATGGGAG CAGTACACCG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATTGC CGTGTCGGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATCTC AGATGTGCTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AAGATGTGCT CCATTCGTTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATAGCAGCCA TCGTGGTCAA GAGACCCCGG 
AAGGTTCAGG TGGCCCCACT GCAAGGCAGC CCCCCTCTCA GTCATGACGA CCGAGGTTTT 
GAAGTGATTG AAGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGTCGG 
CACAGCAGCC ACGACATGCT CAGCCACAGC TGGGAGGGCA ATAGGGAGAG GGGGCGACCC 
CATCAGCGCA CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCAGGAGCCT GGAACGGGGC 
CTGGACCAGG AAGACTATGG GCGCAGCCGT GAGCGCAGCC GTGGCCGGAG CCTGGAGCGC 
GGGCTGGACC GCGACTTTGT GTCCAGGGAC CACAGCCGTG GCCGTAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC GAAGCTTATG AACCCGACTA CGGAGGCGGC 
TACAGCCCAT CATATGACCG TAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGTTCCCG AAGCCCCAGC CCCGAGTCTA GGTCACGGCA TGAACACAAG 
GGCCAACATG ATCCCGACAG GCCCATCGGG GTCCTTCTAA CCAAAAGCAA AGCAAATGAA 
GAGTATGGGC TCCGGCTGGG GAGTCAGATC TTCATCAAAG AAATGACCAG AACAGGACTG 
GCCACCAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA CGGCACTGTT 
ACTGAGAACA TGTCTCTAAC GGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTTGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCAGC CCTAAACGAC 
AGCGACTCTG AAGTGGAGGA TATCTCGGAA ATAGAGTCCA ACCGGTCTTT CTCTCCAGAG 
GAGAGACGCC AGCAGTATTC TGATCAGGAT TACCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCGAGCT CAAGAGAGGA GACCTCAGGC AGACTATCCA GGATGGGTGC CACTCCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCGGGGGTCA CAGAGGCCAG CAGGGAGCCC 
AGGTACCAGG AAGAAGGCCC AGTTCCTCAA CCCAGAACAG CTCCAAGAGT TTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAG GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAACGACG TTGGGATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCCGC AGAGCAGGAG GGCCTGCAAG AAGGAGACCA GATTCTGAAG 
GTGAACACAC AGGATTTCAG AGGGCTAGTC CGGGAAGATG CGGTCCTCTA CCTGCTAGAA 
ATCCCCAAAG GTGAAACTGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTACAGAGAT 
ATCCTGGCTT GTGGCAGGGG AGATTCGTTT TTCATAAGAA GCCACTTTGA ATGTGAGAAG 
GAAACTCCCC AGAGCCTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AGCTGGGCCA CTGGCTGGCC GTGAGGATCG GGAATGAGCT GGAGAAAGGC 
TTGATCCCTA ACAAAAGCAG AGCCGAGCAA ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AACGCCGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCAG CGGCGGCGTC 
AAGAAGAACC TCCGCAAGAG CCGGGAAGAC CTGGCGGCTG CTGTATCGGT TAGCACCAAG 
TTCCCTGCCT ACGAGAAGGT TCTGCTTCGG GAAGCTGGCT TCAAGAGACC CGTGGTTCTG 
TTTGGCCCCA TAGCAGATAT AGCAATGGAA AGGTTGGCAA CTGAGTTACC AGACCTGTTT 
CAAACTGCAA AAACAGAACC CAAAGATGCC GGATCTGAGA AATCCAGTGG AGTGGTTCGG 
TTGAATACCG TGAGGCAAAT TATTGAGCAG GACAAGCACG CCCTGCTCGA CGTTACCCCC 
AAAGCTGTGG ACCTGCTCAA CTACACTCAG TGGTTCCCTA TCGTGATTTT CTTCAACCCG 
GATTCCAGAC AAGGTGTTAA AACCATAAGG CAGAGGTTGA GTCCAACATC CAATAAAAGT 
TCTCGCAAGT TATTTGATCA AGCCAACAAG CTCAAAAAGA CCTGTTCTCA TCTTTTTACA 
GCCACAATCA ACGTGAATTC AGCCAACGAT GGCTGGTTTG GCAGCTTGAA GGACAGCATT 
CAGCAGCAGC AAAACGAAGC AGTCTGGGTC TCTGAAGGAA AGATGGAAGG GATGGATGAT 
GATGCTGAAG ACCGCATGTC CTACTTAACC GCCATGGGCG CGGACTATCT GAGTTGTGAC 
AGCCGTCTCA TCAGTGACTT TGAAGACACC GACGGCGAGG GAGGCGCCTA CACTGACAAT 
GAGCTAGAGG AGCCGGCGGA GGAGCCGCTG GTGTCTTCCA TCACCCGCTC CTCGGAGCCG 
GTGCAGCACG AGGAGGCAAG AAGCCAGAAC AGAGAAGACT CCTTCGACTA CTCCAAGTCA 
AACCTCCCCG CCACAGCCGG CAGTGAAATC CCGGGGGGAT CCACCAAAGG CTATCCTCCC 
CCTATTGCAG CGAAGCCTGC CTTTGGGCGG CCCATCCTGA AGCCTTCCAC TCCAGTCCCT 
ATGCCTGAGA GCGAGGAGGT TGGAGAGAGC ACCGAGGAGC AGGAAGATGC TCCCAGATCA 
GTCCTGGGCA GAGTGAAAAT ATTCGAGAAG ATGGACCACA AGGCAAAATT ACAGAGGATG 
CAGGAGCTCC AGGAGGCCCA GAATGCGAGG ATCGAAATAG CTCAGAAGCA CCCTGACATC 
TATGCGGTTC CAATCAAGGC CCCCAAGCCA GATGCTGGCC TCCCCCCGCA CATGAGTTCT 
AGACCCCCAG AGCCCCAGAA AGCTCCTTCC AGACTTTACC AGGACACCAG AGGAAGCTAC 
GGCAGTGACC CCGAGGAGGA GGAGTACCGC CAGCAGTTGG CAGCACACTC GAAGCGTGGT 
TACTACAGCC AGCCCTCCCG GTACCGAGAC ACGGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( NM_001198985.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185914.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant 1, mRNA.

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CloneID	OMu15570
Clone ID Related Accession (Same CDS sequence)	NM_001198985.1 , NM_001198985.2
Accession Version	NM_001198985.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB724174.1, AK028494.1, CN527767.1, CN529621.1, CF736959.1, BM224980.2, BP767126.1 and BQ176958.1. Transcript Variant: Thsi variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF113005.1, SRR1660811.22433.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CCGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGTCCAGGCA TGGAGGAGGT GATATGGGAG CAGTACACCG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATTGC CGTGTCGGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATCTC AGATGTGCTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AAGATGTGCT CCATTCGTTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATAGCAGCCA TCGTGGTCAA GAGACCCCGG 
AAGGTTCAGG TGGCCCCACT GCAAGGCAGC CCCCCTCTCA GTCATGACGA CCGAGGTTTT 
GAAGTGATTG AAGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGTCGG 
CACAGCAGCC ACGACATGCT CAGCCACAGC TGGGAGGGCA ATAGGGAGAG GGGGCGACCC 
CATCAGCGCA CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCAGGAGCCT GGAACGGGGC 
CTGGACCAGG AAGACTATGG GCGCAGCCGT GAGCGCAGCC GTGGCCGGAG CCTGGAGCGC 
GGGCTGGACC GCGACTTTGT GTCCAGGGAC CACAGCCGTG GCCGTAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC GAAGCTTATG AACCCGACTA CGGAGGCGGC 
TACAGCCCAT CATATGACCG TAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGTTCCCG AAGCCCCAGC CCCGAGTCTA GGTCACGGCA TGAACACAAG 
GGCCAACATG ATCCCGACAG GCCCATCGGG GTCCTTCTAA CCAAAAGCAA AGCAAATGAA 
GAGTATGGGC TCCGGCTGGG GAGTCAGATC TTCATCAAAG AAATGACCAG AACAGGACTG 
GCCACCAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA CGGCACTGTT 
ACTGAGAACA TGTCTCTAAC GGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTTGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCAGC CCTAAACGAC 
AGCGACTCTG AAGTGGAGGA TATCTCGGAA ATAGAGTCCA ACCGGTCTTT CTCTCCAGAG 
GAGAGACGCC AGCAGTATTC TGATCAGGAT TACCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCGAGCT CAAGAGAGGA GACCTCAGGC AGACTATCCA GGATGGGTGC CACTCCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCGGGGGTCA CAGAGGCCAG CAGGGAGCCC 
AGGTACCAGG AAGAAGGCCC AGTTCCTCAA CCCAGAACAG CTCCAAGAGT TTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAG GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAACGACG TTGGGATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCCGC AGAGCAGGAG GGCCTGCAAG AAGGAGACCA GATTCTGAAG 
GTGAACACAC AGGATTTCAG AGGGCTAGTC CGGGAAGATG CGGTCCTCTA CCTGCTAGAA 
ATCCCCAAAG GTGAAACTGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTACAGAGAT 
ATCCTGGCTT GTGGCAGGGG AGATTCGTTT TTCATAAGAA GCCACTTTGA ATGTGAGAAG 
GAAACTCCCC AGAGCCTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AGCTGGGCCA CTGGCTGGCC GTGAGGATCG GGAATGAGCT GGAGAAAGGC 
TTGATCCCTA ACAAAAGCAG AGCCGAGCAA ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AACGCCGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCAG CGGCGGCGTC 
AAGAAGAACC TCCGCAAGAG CCGGGAAGAC CTGGCGGCTG CTGTATCGGT TAGCACCAAG 
TTCCCTGCCT ACGAGAAGGT TCTGCTTCGG GAAGCTGGCT TCAAGAGACC CGTGGTTCTG 
TTTGGCCCCA TAGCAGATAT AGCAATGGAA AGGTTGGCAA CTGAGTTACC AGACCTGTTT 
CAAACTGCAA AAACAGAACC CAAAGATGCC GGATCTGAGA AATCCAGTGG AGTGGTTCGG 
TTGAATACCG TGAGGCAAAT TATTGAGCAG GACAAGCACG CCCTGCTCGA CGTTACCCCC 
AAAGCTGTGG ACCTGCTCAA CTACACTCAG TGGTTCCCTA TCGTGATTTT CTTCAACCCG 
GATTCCAGAC AAGGTGTTAA AACCATAAGG CAGAGGTTGA GTCCAACATC CAATAAAAGT 
TCTCGCAAGT TATTTGATCA AGCCAACAAG CTCAAAAAGA CCTGTTCTCA TCTTTTTACA 
GCCACAATCA ACGTGAATTC AGCCAACGAT GGCTGGTTTG GCAGCTTGAA GGACAGCATT 
CAGCAGCAGC AAAACGAAGC AGTCTGGGTC TCTGAAGGAA AGATGGAAGG GATGGATGAT 
GATGCTGAAG ACCGCATGTC CTACTTAACC GCCATGGGCG CGGACTATCT GAGTTGTGAC 
AGCCGTCTCA TCAGTGACTT TGAAGACACC GACGGCGAGG GAGGCGCCTA CACTGACAAT 
GAGCTAGAGG AGCCGGCGGA GGAGCCGCTG GTGTCTTCCA TCACCCGCTC CTCGGAGCCG 
GTGCAGCACG AGGAGAACAT AAGGAAATCC AGCCCAGAGC CACGAGCTCA GATGAGGAGG 
GCTGCTAGCA GAGACCAACT TAGGGATGCT AGCCCGCCCC CAGCCTTCAA GCCAGAGCCG 
CCCAAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185914.1
CDS	127..3699
Translation	MPVRGDRGFPPRRELSGWQRGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_001198985.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198985.1

ORF Insert Sequence:

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CCGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGTCCAGGCA TGGAGGAGGT GATATGGGAG CAGTACACCG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATTGC CGTGTCGGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATCTC AGATGTGCTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AAGATGTGCT CCATTCGTTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATAGCAGCCA TCGTGGTCAA GAGACCCCGG 
AAGGTTCAGG TGGCCCCACT GCAAGGCAGC CCCCCTCTCA GTCATGACGA CCGAGGTTTT 
GAAGTGATTG AAGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGTCGG 
CACAGCAGCC ACGACATGCT CAGCCACAGC TGGGAGGGCA ATAGGGAGAG GGGGCGACCC 
CATCAGCGCA CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCAGGAGCCT GGAACGGGGC 
CTGGACCAGG AAGACTATGG GCGCAGCCGT GAGCGCAGCC GTGGCCGGAG CCTGGAGCGC 
GGGCTGGACC GCGACTTTGT GTCCAGGGAC CACAGCCGTG GCCGTAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC GAAGCTTATG AACCCGACTA CGGAGGCGGC 
TACAGCCCAT CATATGACCG TAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGTTCCCG AAGCCCCAGC CCCGAGTCTA GGTCACGGCA TGAACACAAG 
GGCCAACATG ATCCCGACAG GCCCATCGGG GTCCTTCTAA CCAAAAGCAA AGCAAATGAA 
GAGTATGGGC TCCGGCTGGG GAGTCAGATC TTCATCAAAG AAATGACCAG AACAGGACTG 
GCCACCAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA CGGCACTGTT 
ACTGAGAACA TGTCTCTAAC GGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTTGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCAGC CCTAAACGAC 
AGCGACTCTG AAGTGGAGGA TATCTCGGAA ATAGAGTCCA ACCGGTCTTT CTCTCCAGAG 
GAGAGACGCC AGCAGTATTC TGATCAGGAT TACCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCGAGCT CAAGAGAGGA GACCTCAGGC AGACTATCCA GGATGGGTGC CACTCCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCGGGGGTCA CAGAGGCCAG CAGGGAGCCC 
AGGTACCAGG AAGAAGGCCC AGTTCCTCAA CCCAGAACAG CTCCAAGAGT TTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAG GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAACGACG TTGGGATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCCGC AGAGCAGGAG GGCCTGCAAG AAGGAGACCA GATTCTGAAG 
GTGAACACAC AGGATTTCAG AGGGCTAGTC CGGGAAGATG CGGTCCTCTA CCTGCTAGAA 
ATCCCCAAAG GTGAAACTGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTACAGAGAT 
ATCCTGGCTT GTGGCAGGGG AGATTCGTTT TTCATAAGAA GCCACTTTGA ATGTGAGAAG 
GAAACTCCCC AGAGCCTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AGCTGGGCCA CTGGCTGGCC GTGAGGATCG GGAATGAGCT GGAGAAAGGC 
TTGATCCCTA ACAAAAGCAG AGCCGAGCAA ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AACGCCGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCAG CGGCGGCGTC 
AAGAAGAACC TCCGCAAGAG CCGGGAAGAC CTGGCGGCTG CTGTATCGGT TAGCACCAAG 
TTCCCTGCCT ACGAGAAGGT TCTGCTTCGG GAAGCTGGCT TCAAGAGACC CGTGGTTCTG 
TTTGGCCCCA TAGCAGATAT AGCAATGGAA AGGTTGGCAA CTGAGTTACC AGACCTGTTT 
CAAACTGCAA AAACAGAACC CAAAGATGCC GGATCTGAGA AATCCAGTGG AGTGGTTCGG 
TTGAATACCG TGAGGCAAAT TATTGAGCAG GACAAGCACG CCCTGCTCGA CGTTACCCCC 
AAAGCTGTGG ACCTGCTCAA CTACACTCAG TGGTTCCCTA TCGTGATTTT CTTCAACCCG 
GATTCCAGAC AAGGTGTTAA AACCATAAGG CAGAGGTTGA GTCCAACATC CAATAAAAGT 
TCTCGCAAGT TATTTGATCA AGCCAACAAG CTCAAAAAGA CCTGTTCTCA TCTTTTTACA 
GCCACAATCA ACGTGAATTC AGCCAACGAT GGCTGGTTTG GCAGCTTGAA GGACAGCATT 
CAGCAGCAGC AAAACGAAGC AGTCTGGGTC TCTGAAGGAA AGATGGAAGG GATGGATGAT 
GATGCTGAAG ACCGCATGTC CTACTTAACC GCCATGGGCG CGGACTATCT GAGTTGTGAC 
AGCCGTCTCA TCAGTGACTT TGAAGACACC GACGGCGAGG GAGGCGCCTA CACTGACAAT 
GAGCTAGAGG AGCCGGCGGA GGAGCCGCTG GTGTCTTCCA TCACCCGCTC CTCGGAGCCG 
GTGCAGCACG AGGAGAACAT AAGGAAATCC AGCCCAGAGC CACGAGCTCA GATGAGGAGG 
GCTGCTAGCA GAGACCAACT TAGGGATGCT AGCCCGCCCC CAGCCTTCAA GCCAGAGCCG 
CCCAAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( NM_001198985.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185914.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15570
Clone ID Related Accession (Same CDS sequence)	NM_001198985.1 , NM_001198985.2
Accession Version	NM_001198985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149224.7, CB724174.1, AK028494.1, CN527767.1, CN529621.1, CF736959.1, BM224980.2, BP767126.1 and BQ176958.1. On Feb 3, 2018 this sequence version replaced NM_001198985.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF113005.1, SRR1660811.22433.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CCGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGTCCAGGCA TGGAGGAGGT GATATGGGAG CAGTACACCG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATTGC CGTGTCGGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATCTC AGATGTGCTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AAGATGTGCT CCATTCGTTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATAGCAGCCA TCGTGGTCAA GAGACCCCGG 
AAGGTTCAGG TGGCCCCACT GCAAGGCAGC CCCCCTCTCA GTCATGACGA CCGAGGTTTT 
GAAGTGATTG AAGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGTCGG 
CACAGCAGCC ACGACATGCT CAGCCACAGC TGGGAGGGCA ATAGGGAGAG GGGGCGACCC 
CATCAGCGCA CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCAGGAGCCT GGAACGGGGC 
CTGGACCAGG AAGACTATGG GCGCAGCCGT GAGCGCAGCC GTGGCCGGAG CCTGGAGCGC 
GGGCTGGACC GCGACTTTGT GTCCAGGGAC CACAGCCGTG GCCGTAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC GAAGCTTATG AACCCGACTA CGGAGGCGGC 
TACAGCCCAT CATATGACCG TAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGTTCCCG AAGCCCCAGC CCCGAGTCTA GGTCACGGCA TGAACACAAG 
GGCCAACATG ATCCCGACAG GCCCATCGGG GTCCTTCTAA CCAAAAGCAA AGCAAATGAA 
GAGTATGGGC TCCGGCTGGG GAGTCAGATC TTCATCAAAG AAATGACCAG AACAGGACTG 
GCCACCAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA CGGCACTGTT 
ACTGAGAACA TGTCTCTAAC GGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTTGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCAGC CCTAAACGAC 
AGCGACTCTG AAGTGGAGGA TATCTCGGAA ATAGAGTCCA ACCGGTCTTT CTCTCCAGAG 
GAGAGACGCC AGCAGTATTC TGATCAGGAT TACCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCGAGCT CAAGAGAGGA GACCTCAGGC AGACTATCCA GGATGGGTGC CACTCCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCGGGGGTCA CAGAGGCCAG CAGGGAGCCC 
AGGTACCAGG AAGAAGGCCC AGTTCCTCAA CCCAGAACAG CTCCAAGAGT TTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAG GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAACGACG TTGGGATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCCGC AGAGCAGGAG GGCCTGCAAG AAGGAGACCA GATTCTGAAG 
GTGAACACAC AGGATTTCAG AGGGCTAGTC CGGGAAGATG CGGTCCTCTA CCTGCTAGAA 
ATCCCCAAAG GTGAAACTGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTACAGAGAT 
ATCCTGGCTT GTGGCAGGGG AGATTCGTTT TTCATAAGAA GCCACTTTGA ATGTGAGAAG 
GAAACTCCCC AGAGCCTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AGCTGGGCCA CTGGCTGGCC GTGAGGATCG GGAATGAGCT GGAGAAAGGC 
TTGATCCCTA ACAAAAGCAG AGCCGAGCAA ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AACGCCGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCAG CGGCGGCGTC 
AAGAAGAACC TCCGCAAGAG CCGGGAAGAC CTGGCGGCTG CTGTATCGGT TAGCACCAAG 
TTCCCTGCCT ACGAGAAGGT TCTGCTTCGG GAAGCTGGCT TCAAGAGACC CGTGGTTCTG 
TTTGGCCCCA TAGCAGATAT AGCAATGGAA AGGTTGGCAA CTGAGTTACC AGACCTGTTT 
CAAACTGCAA AAACAGAACC CAAAGATGCC GGATCTGAGA AATCCAGTGG AGTGGTTCGG 
TTGAATACCG TGAGGCAAAT TATTGAGCAG GACAAGCACG CCCTGCTCGA CGTTACCCCC 
AAAGCTGTGG ACCTGCTCAA CTACACTCAG TGGTTCCCTA TCGTGATTTT CTTCAACCCG 
GATTCCAGAC AAGGTGTTAA AACCATAAGG CAGAGGTTGA GTCCAACATC CAATAAAAGT 
TCTCGCAAGT TATTTGATCA AGCCAACAAG CTCAAAAAGA CCTGTTCTCA TCTTTTTACA 
GCCACAATCA ACGTGAATTC AGCCAACGAT GGCTGGTTTG GCAGCTTGAA GGACAGCATT 
CAGCAGCAGC AAAACGAAGC AGTCTGGGTC TCTGAAGGAA AGATGGAAGG GATGGATGAT 
GATGCTGAAG ACCGCATGTC CTACTTAACC GCCATGGGCG CGGACTATCT GAGTTGTGAC 
AGCCGTCTCA TCAGTGACTT TGAAGACACC GACGGCGAGG GAGGCGCCTA CACTGACAAT 
GAGCTAGAGG AGCCGGCGGA GGAGCCGCTG GTGTCTTCCA TCACCCGCTC CTCGGAGCCG 
GTGCAGCACG AGGAGAACAT AAGGAAATCC AGCCCAGAGC CACGAGCTCA GATGAGGAGG 
GCTGCTAGCA GAGACCAACT TAGGGATGCT AGCCCGCCCC CAGCCTTCAA GCCAGAGCCG 
CCCAAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185914.1
CDS	176..3748
Translation	MPVRGDRGFPPRRELSGWQRGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_001198985.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198985.2

ORF Insert Sequence:

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CCGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGTCCAGGCA TGGAGGAGGT GATATGGGAG CAGTACACCG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATTGC CGTGTCGGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATCTC AGATGTGCTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AAGATGTGCT CCATTCGTTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATAGCAGCCA TCGTGGTCAA GAGACCCCGG 
AAGGTTCAGG TGGCCCCACT GCAAGGCAGC CCCCCTCTCA GTCATGACGA CCGAGGTTTT 
GAAGTGATTG AAGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGTCGG 
CACAGCAGCC ACGACATGCT CAGCCACAGC TGGGAGGGCA ATAGGGAGAG GGGGCGACCC 
CATCAGCGCA CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCAGGAGCCT GGAACGGGGC 
CTGGACCAGG AAGACTATGG GCGCAGCCGT GAGCGCAGCC GTGGCCGGAG CCTGGAGCGC 
GGGCTGGACC GCGACTTTGT GTCCAGGGAC CACAGCCGTG GCCGTAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC GAAGCTTATG AACCCGACTA CGGAGGCGGC 
TACAGCCCAT CATATGACCG TAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGTTCCCG AAGCCCCAGC CCCGAGTCTA GGTCACGGCA TGAACACAAG 
GGCCAACATG ATCCCGACAG GCCCATCGGG GTCCTTCTAA CCAAAAGCAA AGCAAATGAA 
GAGTATGGGC TCCGGCTGGG GAGTCAGATC TTCATCAAAG AAATGACCAG AACAGGACTG 
GCCACCAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA CGGCACTGTT 
ACTGAGAACA TGTCTCTAAC GGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTTGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCAGC CCTAAACGAC 
AGCGACTCTG AAGTGGAGGA TATCTCGGAA ATAGAGTCCA ACCGGTCTTT CTCTCCAGAG 
GAGAGACGCC AGCAGTATTC TGATCAGGAT TACCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCGAGCT CAAGAGAGGA GACCTCAGGC AGACTATCCA GGATGGGTGC CACTCCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCGGGGGTCA CAGAGGCCAG CAGGGAGCCC 
AGGTACCAGG AAGAAGGCCC AGTTCCTCAA CCCAGAACAG CTCCAAGAGT TTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAG GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAACGACG TTGGGATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCCGC AGAGCAGGAG GGCCTGCAAG AAGGAGACCA GATTCTGAAG 
GTGAACACAC AGGATTTCAG AGGGCTAGTC CGGGAAGATG CGGTCCTCTA CCTGCTAGAA 
ATCCCCAAAG GTGAAACTGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTACAGAGAT 
ATCCTGGCTT GTGGCAGGGG AGATTCGTTT TTCATAAGAA GCCACTTTGA ATGTGAGAAG 
GAAACTCCCC AGAGCCTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AGCTGGGCCA CTGGCTGGCC GTGAGGATCG GGAATGAGCT GGAGAAAGGC 
TTGATCCCTA ACAAAAGCAG AGCCGAGCAA ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AACGCCGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCAG CGGCGGCGTC 
AAGAAGAACC TCCGCAAGAG CCGGGAAGAC CTGGCGGCTG CTGTATCGGT TAGCACCAAG 
TTCCCTGCCT ACGAGAAGGT TCTGCTTCGG GAAGCTGGCT TCAAGAGACC CGTGGTTCTG 
TTTGGCCCCA TAGCAGATAT AGCAATGGAA AGGTTGGCAA CTGAGTTACC AGACCTGTTT 
CAAACTGCAA AAACAGAACC CAAAGATGCC GGATCTGAGA AATCCAGTGG AGTGGTTCGG 
TTGAATACCG TGAGGCAAAT TATTGAGCAG GACAAGCACG CCCTGCTCGA CGTTACCCCC 
AAAGCTGTGG ACCTGCTCAA CTACACTCAG TGGTTCCCTA TCGTGATTTT CTTCAACCCG 
GATTCCAGAC AAGGTGTTAA AACCATAAGG CAGAGGTTGA GTCCAACATC CAATAAAAGT 
TCTCGCAAGT TATTTGATCA AGCCAACAAG CTCAAAAAGA CCTGTTCTCA TCTTTTTACA 
GCCACAATCA ACGTGAATTC AGCCAACGAT GGCTGGTTTG GCAGCTTGAA GGACAGCATT 
CAGCAGCAGC AAAACGAAGC AGTCTGGGTC TCTGAAGGAA AGATGGAAGG GATGGATGAT 
GATGCTGAAG ACCGCATGTC CTACTTAACC GCCATGGGCG CGGACTATCT GAGTTGTGAC 
AGCCGTCTCA TCAGTGACTT TGAAGACACC GACGGCGAGG GAGGCGCCTA CACTGACAAT 
GAGCTAGAGG AGCCGGCGGA GGAGCCGCTG GTGTCTTCCA TCACCCGCTC CTCGGAGCCG 
GTGCAGCACG AGGAGAACAT AAGGAAATCC AGCCCAGAGC CACGAGCTCA GATGAGGAGG 
GCTGCTAGCA GAGACCAACT TAGGGATGCT AGCCCGCCCC CAGCCTTCAA GCCAGAGCCG 
CCCAAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( NM_011597.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035727.2 Mus musculus tight junction protein 2 (Tjp2), transcript variant 2, mRNA.

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CloneID	OMu15700
Clone ID Related Accession (Same CDS sequence)	NM_011597.4 , XM_011247219.2 , NM_011597.5 , NM_001376368.1 , XM_006526908.3
Accession Version	NM_011597.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY032291.1, CB724174.1, AK028494.1, CN527767.1, CN529621.1, CF736959.1, BM224980.2, BP767126.1 and BQ176958.1. On Nov 17, 2010 this sequence version replaced NM_011597.3. Transcript Variant: This variant (2) has a different 5' terminal exon which results in the use of a downstream translation initiation site, compared to variant 1. Variant 2 encodes an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF113005.1, BC042695.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035727.2
CDS	186..3689
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_011597.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011597.4

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( NM_011597.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035727.2 Mus musculus tight junction protein 2 (Tjp2), transcript variant 2, mRNA.

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CloneID	OMu15700
Clone ID Related Accession (Same CDS sequence)	NM_011597.4 , XM_011247219.2 , NM_011597.5 , NM_001376368.1 , XM_006526908.3
Accession Version	NM_011597.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-10
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149224.7, BY032291.1, CB724174.1, AK028494.1, CN527767.1, CN529621.1, CF736959.1, BM224980.2, BP767126.1 and BQ176958.1. On Feb 3, 2018 this sequence version replaced NM_011597.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035727.2
CDS	288..3791
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_011597.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011597.5

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( NM_001360391.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347320.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant 3, mRNA.

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CloneID	OMu55412
Clone ID Related Accession (Same CDS sequence)	XM_006526905.2 , NM_001360391.1
Accession Version	NM_001360391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149224.7, AC107685.7 and BQ176958.1. On Feb 3, 2018 this sequence version replaced XM_006526905.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660819.61132.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGAAC ATAAGGAAAT CCAGCCCAGA GCCACGAGCT 
CAGATGAGGA GGGCTGCTAG CAGAGACCAA CTTAGGGATG CTAGCCCGCC CCCAGCCTTC 
AAGCCAGAGC CGCCCAAGGC AAGAAGCCAG AACAGAGAAG ACTCCTTCGA CTACTCCAAG 
TCAAACCTCC CCGCCACAGC CGGCAGTGAA ATCCCGGGGG GATCCACCAA AGGCTATCCT 
CCCCCTATTG CAGCGAAGCC TGCCTTTGGG CGGCCCATCC TGAAGCCTTC CACTCCAGTC 
CCTATGCCTG AGAGCGAGGA GGTTGGAGAG AGCACCGAGG AGCAGGAAGA TGCTCCCAGA 
TCAGTCCTGG GCAGAGTGAA AATATTCGAG AAGATGGACC ACAAGGCAAA ATTACAGAGG 
ATGCAGGAGC TCCAGGAGGC CCAGAATGCG AGGATCGAAA TAGCTCAGAA GCACCCTGAC 
ATCTATGCGG TTCCAATCAA GGCCCCCAAG CCAGATGCTG GCCTCCCCCC GCACATGAGT 
TCTAGACCCC CAGAGCCCCA GAAAGCTCCT TCCAGACTTT ACCAGGACAC CAGAGGAAGC 
TACGGCAGTG ACCCCGAGGA GGAGGAGTAC CGCCAGCAGT TGGCAGCACA CTCGAAGCGT 
GGTTACTACA GCCAGCCCTC CCGGTACCGA GACACGGAGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347320.1
CDS	229..3813
Translation	MRASQVLQRMWIQAVKKLERWKGHGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_001360391.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360391.1

ORF Insert Sequence:

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGAAC ATAAGGAAAT CCAGCCCAGA GCCACGAGCT 
CAGATGAGGA GGGCTGCTAG CAGAGACCAA CTTAGGGATG CTAGCCCGCC CCCAGCCTTC 
AAGCCAGAGC CGCCCAAGGC AAGAAGCCAG AACAGAGAAG ACTCCTTCGA CTACTCCAAG 
TCAAACCTCC CCGCCACAGC CGGCAGTGAA ATCCCGGGGG GATCCACCAA AGGCTATCCT 
CCCCCTATTG CAGCGAAGCC TGCCTTTGGG CGGCCCATCC TGAAGCCTTC CACTCCAGTC 
CCTATGCCTG AGAGCGAGGA GGTTGGAGAG AGCACCGAGG AGCAGGAAGA TGCTCCCAGA 
TCAGTCCTGG GCAGAGTGAA AATATTCGAG AAGATGGACC ACAAGGCAAA ATTACAGAGG 
ATGCAGGAGC TCCAGGAGGC CCAGAATGCG AGGATCGAAA TAGCTCAGAA GCACCCTGAC 
ATCTATGCGG TTCCAATCAA GGCCCCCAAG CCAGATGCTG GCCTCCCCCC GCACATGAGT 
TCTAGACCCC CAGAGCCCCA GAAAGCTCCT TCCAGACTTT ACCAGGACAC CAGAGGAAGC 
TACGGCAGTG ACCCCGAGGA GGAGGAGTAC CGCCAGCAGT TGGCAGCACA CTCGAAGCGT 
GGTTACTACA GCCAGCCCTC CCGGTACCGA GACACGGAGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( NM_001360392.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347321.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant 4, mRNA.

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CloneID	OMu79931
Clone ID Related Accession (Same CDS sequence)	XM_017318137.1 , NM_001360392.1 , XM_017318136.1
Accession Version	NM_001360392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149224.7, BY073294.1, AC107685.7 and BQ176958.1. On Feb 3, 2018 this sequence version replaced XM_017318137.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.137328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347321.1
CDS	409..3801
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_001360392.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360392.1

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_011247219.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245521.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.

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CloneID	OMu15700
Clone ID Related Accession (Same CDS sequence)	NM_011597.4 , XM_011247219.2 , NM_011597.5 , NM_001376368.1 , XM_006526908.3
Accession Version	XM_011247219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245521.1
CDS	177..3680
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_011247219.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247219.2

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526908.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526971.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu15700
Clone ID Related Accession (Same CDS sequence)	NM_011597.4 , XM_011247219.2 , NM_011597.5 , NM_001376368.1 , XM_006526908.3
Accession Version	XM_006526908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526971.1
CDS	410..3913
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526908.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526908.3

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGAACA TAAGGAAATC CAGCCCAGAG CCACGAGCTC AGATGAGGAG GGCTGCTAGC 
AGAGACCAAC TTAGGGATGC TAGCCCGCCC CCAGCCTTCA AGCCAGAGCC GCCCAAGGCA 
AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC TACTCCAAGT CAAACCTCCC CGCCACAGCC 
GGCAGTGAAA TCCCGGGGGG ATCCACCAAA GGCTATCCTC CCCCTATTGC AGCGAAGCCT 
GCCTTTGGGC GGCCCATCCT GAAGCCTTCC ACTCCAGTCC CTATGCCTGA GAGCGAGGAG 
GTTGGAGAGA GCACCGAGGA GCAGGAAGAT GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA 
ATATTCGAGA AGATGGACCA CAAGGCAAAA TTACAGAGGA TGCAGGAGCT CCAGGAGGCC 
CAGAATGCGA GGATCGAAAT AGCTCAGAAG CACCCTGACA TCTATGCGGT TCCAATCAAG 
GCCCCCAAGC CAGATGCTGG CCTCCCCCCG CACATGAGTT CTAGACCCCC AGAGCCCCAG 
AAAGCTCCTT CCAGACTTTA CCAGGACACC AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG 
GAGGAGTACC GCCAGCAGTT GGCAGCACAC TCGAAGCGTG GTTACTACAG CCAGCCCTCC 
CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526904.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526967.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.

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CloneID	OMu55411
Clone ID Related Accession (Same CDS sequence)	XM_006526904.4 , XM_006526904.3
Accession Version	XM_006526904.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGA ACATAAGGAA ATCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT 
AGCAGAGACC AACTTAGGGA TGCTAGCCCG CCCCCAGCCT TCAAGCCAGA GCCGCCCAAG 
GCAAGAAGCC AGAACAGAGA AGACTCCTTC GACTACTCCA AGTCAAACCT CCCCGCCACA 
GCCGGCAGTG AAATCCCGGG GGGATCCACC AAAGGCTATC CTCCCCCTAT TGCAGCGAAG 
CCTGCCTTTG GGCGGCCCAT CCTGAAGCCT TCCACTCCAG TCCCTATGCC TGAGAGCGAG 
GAGGTTGGAG AGAGCACCGA GGAGCAGGAA GATGCTCCCA GATCAGTCCT GGGCAGAGTG 
AAAATATTCG AGAAGATGGA CCACAAGGCA AAATTACAGA GGATGCAGGA GCTCCAGGAG 
GCCCAGAATG CGAGGATCGA AATAGCTCAG AAGCACCCTG ACATCTATGC GGTTCCAATC 
AAGGCCCCCA AGCCAGATGC TGGCCTCCCC CCGCACATGA GTTCTAGACC CCCAGAGCCC 
CAGAAAGCTC CTTCCAGACT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGACCCCGAG 
GAGGAGGAGT ACCGCCAGCA GTTGGCAGCA CACTCGAAGC GTGGTTACTA CAGCCAGCCC 
TCCCGGTACC GAGACACGGA GTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526967.1
CDS	207..3833
Translation	MTRGRRKGKEEKMPVFRRSTNSLSSGPGKKQALRTTGKGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526904.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526904.3

ORF Insert Sequence:

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGA ACATAAGGAA ATCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT 
AGCAGAGACC AACTTAGGGA TGCTAGCCCG CCCCCAGCCT TCAAGCCAGA GCCGCCCAAG 
GCAAGAAGCC AGAACAGAGA AGACTCCTTC GACTACTCCA AGTCAAACCT CCCCGCCACA 
GCCGGCAGTG AAATCCCGGG GGGATCCACC AAAGGCTATC CTCCCCCTAT TGCAGCGAAG 
CCTGCCTTTG GGCGGCCCAT CCTGAAGCCT TCCACTCCAG TCCCTATGCC TGAGAGCGAG 
GAGGTTGGAG AGAGCACCGA GGAGCAGGAA GATGCTCCCA GATCAGTCCT GGGCAGAGTG 
AAAATATTCG AGAAGATGGA CCACAAGGCA AAATTACAGA GGATGCAGGA GCTCCAGGAG 
GCCCAGAATG CGAGGATCGA AATAGCTCAG AAGCACCCTG ACATCTATGC GGTTCCAATC 
AAGGCCCCCA AGCCAGATGC TGGCCTCCCC CCGCACATGA GTTCTAGACC CCCAGAGCCC 
CAGAAAGCTC CTTCCAGACT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGACCCCGAG 
GAGGAGGAGT ACCGCCAGCA GTTGGCAGCA CACTCGAAGC GTGGTTACTA CAGCCAGCCC 
TCCCGGTACC GAGACACGGA GTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526905.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526968.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.

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CloneID	OMu55412
Clone ID Related Accession (Same CDS sequence)	XM_006526905.2 , NM_001360391.1
Accession Version	XM_006526905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGAAC ATAAGGAAAT CCAGCCCAGA GCCACGAGCT 
CAGATGAGGA GGGCTGCTAG CAGAGACCAA CTTAGGGATG CTAGCCCGCC CCCAGCCTTC 
AAGCCAGAGC CGCCCAAGGC AAGAAGCCAG AACAGAGAAG ACTCCTTCGA CTACTCCAAG 
TCAAACCTCC CCGCCACAGC CGGCAGTGAA ATCCCGGGGG GATCCACCAA AGGCTATCCT 
CCCCCTATTG CAGCGAAGCC TGCCTTTGGG CGGCCCATCC TGAAGCCTTC CACTCCAGTC 
CCTATGCCTG AGAGCGAGGA GGTTGGAGAG AGCACCGAGG AGCAGGAAGA TGCTCCCAGA 
TCAGTCCTGG GCAGAGTGAA AATATTCGAG AAGATGGACC ACAAGGCAAA ATTACAGAGG 
ATGCAGGAGC TCCAGGAGGC CCAGAATGCG AGGATCGAAA TAGCTCAGAA GCACCCTGAC 
ATCTATGCGG TTCCAATCAA GGCCCCCAAG CCAGATGCTG GCCTCCCCCC GCACATGAGT 
TCTAGACCCC CAGAGCCCCA GAAAGCTCCT TCCAGACTTT ACCAGGACAC CAGAGGAAGC 
TACGGCAGTG ACCCCGAGGA GGAGGAGTAC CGCCAGCAGT TGGCAGCACA CTCGAAGCGT 
GGTTACTACA GCCAGCCCTC CCGGTACCGA GACACGGAGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526968.1
CDS	224..3808
Translation	MRASQVLQRMWIQAVKKLERWKGHGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRRAASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526905.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526905.2

ORF Insert Sequence:

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGAAC ATAAGGAAAT CCAGCCCAGA GCCACGAGCT 
CAGATGAGGA GGGCTGCTAG CAGAGACCAA CTTAGGGATG CTAGCCCGCC CCCAGCCTTC 
AAGCCAGAGC CGCCCAAGGC AAGAAGCCAG AACAGAGAAG ACTCCTTCGA CTACTCCAAG 
TCAAACCTCC CCGCCACAGC CGGCAGTGAA ATCCCGGGGG GATCCACCAA AGGCTATCCT 
CCCCCTATTG CAGCGAAGCC TGCCTTTGGG CGGCCCATCC TGAAGCCTTC CACTCCAGTC 
CCTATGCCTG AGAGCGAGGA GGTTGGAGAG AGCACCGAGG AGCAGGAAGA TGCTCCCAGA 
TCAGTCCTGG GCAGAGTGAA AATATTCGAG AAGATGGACC ACAAGGCAAA ATTACAGAGG 
ATGCAGGAGC TCCAGGAGGC CCAGAATGCG AGGATCGAAA TAGCTCAGAA GCACCCTGAC 
ATCTATGCGG TTCCAATCAA GGCCCCCAAG CCAGATGCTG GCCTCCCCCC GCACATGAGT 
TCTAGACCCC CAGAGCCCCA GAAAGCTCCT TCCAGACTTT ACCAGGACAC CAGAGGAAGC 
TACGGCAGTG ACCCCGAGGA GGAGGAGTAC CGCCAGCAGT TGGCAGCACA CTCGAAGCGT 
GGTTACTACA GCCAGCCCTC CCGGTACCGA GACACGGAGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526907.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526970.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X3, mRNA.

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CloneID	OMu55414
Clone ID Related Accession (Same CDS sequence)	XM_006526907.2 , XM_006526907.3
Accession Version	XM_006526907.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006526907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGG CAAGAAGCCA GAACAGAGAA GACTCCTTCG ACTACTCCAA GTCAAACCTC 
CCCGCCACAG CCGGCAGTGA AATCCCGGGG GGATCCACCA AAGGCTATCC TCCCCCTATT 
GCAGCGAAGC CTGCCTTTGG GCGGCCCATC CTGAAGCCTT CCACTCCAGT CCCTATGCCT 
GAGAGCGAGG AGGTTGGAGA GAGCACCGAG GAGCAGGAAG ATGCTCCCAG ATCAGTCCTG 
GGCAGAGTGA AAATATTCGA GAAGATGGAC CACAAGGCAA AATTACAGAG GATGCAGGAG 
CTCCAGGAGG CCCAGAATGC GAGGATCGAA ATAGCTCAGA AGCACCCTGA CATCTATGCG 
GTTCCAATCA AGGCCCCCAA GCCAGATGCT GGCCTCCCCC CGCACATGAG TTCTAGACCC 
CCAGAGCCCC AGAAAGCTCC TTCCAGACTT TACCAGGACA CCAGAGGAAG CTACGGCAGT 
GACCCCGAGG AGGAGGAGTA CCGCCAGCAG TTGGCAGCAC ACTCGAAGCG TGGTTACTAC 
AGCCAGCCCT CCCGGTACCG AGACACGGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526970.1
CDS	139..3654
Translation	MTRGRRKGKEEKMPVFRRSTNSLSSGPGKKQALRTTGKGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526907.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526907.2

ORF Insert Sequence:

ATGACCAGAG GAAGAAGGAA GGGAAAAGAA GAGAAGATGC CTGTGTTCCG CCGCAGCACC 
AACTCGCTCA GCTCAGGCCC AGGAAAAAAG CAGGCTCTGA GGACCACTGG GAAGGGTCCA 
GGCATGGAGG AGGTGATATG GGAGCAGTAC ACCGTGACCC TGCAGAAGGA TTCCAAAAGA 
GGATTTGGAA TTGCCGTGTC GGGAGGCAGA GACAACCCCC ACTTTGAAAA TGGGGAGACG 
TCAATCGTCA TCTCAGATGT GCTCCCAGGT GGACCCGCTG ATGGCTTGCT TCAAGAAAAT 
GACAGGGTGG TCATGGTCAA TGGCACCCCC ATGGAAGATG TGCTCCATTC GTTTGCCGTT 
CAGCAGCTTA GGAAAAGTGG GAAGATAGCA GCCATCGTGG TCAAGAGACC CCGGAAGGTT 
CAGGTGGCCC CACTGCAAGG CAGCCCCCCT CTCAGTCATG ACGACCGAGG TTTTGAAGTG 
ATTGAAGAAT TTGATGGCAG AAGTTTCCGC AGCGGCTACA GTGAGAGGAG TCGGCACAGC 
AGCCACGACA TGCTCAGCCA CAGCTGGGAG GGCAATAGGG AGAGGGGGCG ACCCCATCAG 
CGCACACAGA GCCGGGAGCG GGAGCGCAGC CGTGGCAGGA GCCTGGAACG GGGCCTGGAC 
CAGGAAGACT ATGGGCGCAG CCGTGAGCGC AGCCGTGGCC GGAGCCTGGA GCGCGGGCTG 
GACCGCGACT TTGTGTCCAG GGACCACAGC CGTGGCCGTA GCATCGACAG GGACTACGAC 
CGAGACTATG AGCGCTCCTA TCACGAAGCT TATGAACCCG ACTACGGAGG CGGCTACAGC 
CCATCATATG ACCGTAGAGC CCATCCAGAG ACCCGCTATG AACGGAGCCG CAGCCGAGAA 
CACCTACGTT CCCGAAGCCC CAGCCCCGAG TCTAGGTCAC GGCATGAACA CAAGGGCCAA 
CATGATCCCG ACAGGCCCAT CGGGGTCCTT CTAACCAAAA GCAAAGCAAA TGAAGAGTAT 
GGGCTCCGGC TGGGGAGTCA GATCTTCATC AAAGAAATGA CCAGAACAGG ACTGGCCACC 
AAAGATGGCA ACCTTCATGA GGGGGACATA ATTCTCAAGA TCAACGGCAC TGTTACTGAG 
AACATGTCTC TAACGGATGC TCGGAAGTTA ATAGAAAAGT CTCGAGGAAA ACTGCAGCTT 
GTGGTGTTGA GAGACAGCAA GCAGACCCTC ATCAACATCC CAGCCCTAAA CGACAGCGAC 
TCTGAAGTGG AGGATATCTC GGAAATAGAG TCCAACCGGT CTTTCTCTCC AGAGGAGAGA 
CGCCAGCAGT ATTCTGATCA GGATTACCAT TCCTCCACTG AGAAGCTGAA GGAGAGGCCG 
AGCTCAAGAG AGGAGACCTC AGGCAGACTA TCCAGGATGG GTGCCACTCC CACGCCGTTC 
AAGTCCACGG GGGACATCAC AGCTGCGGGG GTCACAGAGG CCAGCAGGGA GCCCAGGTAC 
CAGGAAGAAG GCCCAGTTCC TCAACCCAGA ACAGCTCCAA GAGTTTTTCT TCGTCCTAGT 
CCCGAAGATG AAGCAATATA TGGCCCTAAC ACCAAAATGG TGAGGTTCAA GAAGGGAGAC 
AGCGTGGGCC TCCGGTTGGC TGGTGGAAAC GACGTTGGGA TATTTGTGGC TGGCATTCAG 
GAGGGCACCT CCGCAGAGCA GGAGGGCCTG CAAGAAGGAG ACCAGATTCT GAAGGTGAAC 
ACACAGGATT TCAGAGGGCT AGTCCGGGAA GATGCGGTCC TCTACCTGCT AGAAATCCCC 
AAAGGTGAAA CTGTGACCAT TTTGGCTCAG AGCCGAGCAG ACGTGTACAG AGATATCCTG 
GCTTGTGGCA GGGGAGATTC GTTTTTCATA AGAAGCCACT TTGAATGTGA GAAGGAAACT 
CCCCAGAGCC TGGCCTTCAC CAGGGGAGAG GTCTTCCGAG TGGTAGACAC GCTGTACGAT 
GGCAAGCTGG GCCACTGGCT GGCCGTGAGG ATCGGGAATG AGCTGGAGAA AGGCTTGATC 
CCTAACAAAA GCAGAGCCGA GCAAATGGCC AGTGTCCAGA ATGCCCAGCG AGAGAACGCC 
GGGGACAGAG CAGACTTCTG GCGGATGCGT GGCCAGAGAT CCAGCGGCGG CGTCAAGAAG 
AACCTCCGCA AGAGCCGGGA AGACCTGGCG GCTGCTGTAT CGGTTAGCAC CAAGTTCCCT 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGTTG GCAACTGAGT TACCAGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCCGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACCGTGAGGC AAATTATTGA GCAGGACAAG CACGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAACTACAC TCAGTGGTTC CCTATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGTG TTAAAACCAT AAGGCAGAGG TTGAGTCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTTG ATCAAGCCAA CAAGCTCAAA AAGACCTGTT CTCATCTTTT TACAGCCACA 
ATCAACGTGA ATTCAGCCAA CGATGGCTGG TTTGGCAGCT TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA CACCGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTA 
GAGGAGCCGG CGGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG 
CACGAGGAGG CAAGAAGCCA GAACAGAGAA GACTCCTTCG ACTACTCCAA GTCAAACCTC 
CCCGCCACAG CCGGCAGTGA AATCCCGGGG GGATCCACCA AAGGCTATCC TCCCCCTATT 
GCAGCGAAGC CTGCCTTTGG GCGGCCCATC CTGAAGCCTT CCACTCCAGT CCCTATGCCT 
GAGAGCGAGG AGGTTGGAGA GAGCACCGAG GAGCAGGAAG ATGCTCCCAG ATCAGTCCTG 
GGCAGAGTGA AAATATTCGA GAAGATGGAC CACAAGGCAA AATTACAGAG GATGCAGGAG 
CTCCAGGAGG CCCAGAATGC GAGGATCGAA ATAGCTCAGA AGCACCCTGA CATCTATGCG 
GTTCCAATCA AGGCCCCCAA GCCAGATGCT GGCCTCCCCC CGCACATGAG TTCTAGACCC 
CCAGAGCCCC AGAAAGCTCC TTCCAGACTT TACCAGGACA CCAGAGGAAG CTACGGCAGT 
GACCCCGAGG AGGAGGAGTA CCGCCAGCAG TTGGCAGCAC ACTCGAAGCG TGGTTACTAC 
AGCCAGCCCT CCCGGTACCG AGACACGGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006526909.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526972.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.

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CloneID	OMu55415
Clone ID Related Accession (Same CDS sequence)	XM_006526909.1 , XM_006526909.2
Accession Version	XM_006526909.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGGCA AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC 
TACTCCAAGT CAAACCTCCC CGCCACAGCC GGCAGTGAAA TCCCGGGGGG ATCCACCAAA 
GGCTATCCTC CCCCTATTGC AGCGAAGCCT GCCTTTGGGC GGCCCATCCT GAAGCCTTCC 
ACTCCAGTCC CTATGCCTGA GAGCGAGGAG GTTGGAGAGA GCACCGAGGA GCAGGAAGAT 
GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA ATATTCGAGA AGATGGACCA CAAGGCAAAA 
TTACAGAGGA TGCAGGAGCT CCAGGAGGCC CAGAATGCGA GGATCGAAAT AGCTCAGAAG 
CACCCTGACA TCTATGCGGT TCCAATCAAG GCCCCCAAGC CAGATGCTGG CCTCCCCCCG 
CACATGAGTT CTAGACCCCC AGAGCCCCAG AAAGCTCCTT CCAGACTTTA CCAGGACACC 
AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG GAGGAGTACC GCCAGCAGTT GGCAGCACAC 
TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526972.1
CDS	224..3697
Translation	MRASQVLQRMWIQAVKKLERWKGHGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006526909.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526909.1

ORF Insert Sequence:

ATGAGGGCCT CTCAGGTCCT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGTCCAGG CATGGAGGAG GTGATATGGG AGCAGTACAC CGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATT GCCGTGTCGG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATC TCAGATGTGC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAAGATGTG 
CTCCATTCGT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATAGCAGC CATCGTGGTC 
AAGAGACCCC GGAAGGTTCA GGTGGCCCCA CTGCAAGGCA GCCCCCCTCT CAGTCATGAC 
GACCGAGGTT TTGAAGTGAT TGAAGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGTC GGCACAGCAG CCACGACATG CTCAGCCACA GCTGGGAGGG CAATAGGGAG 
AGGGGGCGAC CCCATCAGCG CACACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCAGGAGC 
CTGGAACGGG GCCTGGACCA GGAAGACTAT GGGCGCAGCC GTGAGCGCAG CCGTGGCCGG 
AGCCTGGAGC GCGGGCTGGA CCGCGACTTT GTGTCCAGGG ACCACAGCCG TGGCCGTAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACGAAGCTTA TGAACCCGAC 
TACGGAGGCG GCTACAGCCC ATCATATGAC CGTAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGTTCC CGAAGCCCCA GCCCCGAGTC TAGGTCACGG 
CATGAACACA AGGGCCAACA TGATCCCGAC AGGCCCATCG GGGTCCTTCT AACCAAAAGC 
AAAGCAAATG AAGAGTATGG GCTCCGGCTG GGGAGTCAGA TCTTCATCAA AGAAATGACC 
AGAACAGGAC TGGCCACCAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AACGGCACTG TTACTGAGAA CATGTCTCTA ACGGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTTGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
GCCCTAAACG ACAGCGACTC TGAAGTGGAG GATATCTCGG AAATAGAGTC CAACCGGTCT 
TTCTCTCCAG AGGAGAGACG CCAGCAGTAT TCTGATCAGG ATTACCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCGAG CTCAAGAGAG GAGACCTCAG GCAGACTATC CAGGATGGGT 
GCCACTCCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCGGGGGT CACAGAGGCC 
AGCAGGGAGC CCAGGTACCA GGAAGAAGGC CCAGTTCCTC AACCCAGAAC AGCTCCAAGA 
GTTTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAACGA CGTTGGGATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCC GCAGAGCAGG AGGGCCTGCA AGAAGGAGAC 
CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGGCTAG TCCGGGAAGA TGCGGTCCTC 
TACCTGCTAG AAATCCCCAA AGGTGAAACT GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTACAGAG ATATCCTGGC TTGTGGCAGG GGAGATTCGT TTTTCATAAG AAGCCACTTT 
GAATGTGAGA AGGAAACTCC CCAGAGCCTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAGCTGGGC CACTGGCTGG CCGTGAGGAT CGGGAATGAG 
CTGGAGAAAG GCTTGATCCC TAACAAAAGC AGAGCCGAGC AAATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAACGCCGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
AGCGGCGGCG TCAAGAAGAA CCTCCGCAAG AGCCGGGAAG ACCTGGCGGC TGCTGTATCG 
GTTAGCACCA AGTTCCCTGC CTACGAGAAG GTTCTGCTTC GGGAAGCTGG CTTCAAGAGA 
CCCGTGGTTC TGTTTGGCCC CATAGCAGAT ATAGCAATGG AAAGGTTGGC AACTGAGTTA 
CCAGACCTGT TTCAAACTGC AAAAACAGAA CCCAAAGATG CCGGATCTGA GAAATCCAGT 
GGAGTGGTTC GGTTGAATAC CGTGAGGCAA ATTATTGAGC AGGACAAGCA CGCCCTGCTC 
GACGTTACCC CCAAAGCTGT GGACCTGCTC AACTACACTC AGTGGTTCCC TATCGTGATT 
TTCTTCAACC CGGATTCCAG ACAAGGTGTT AAAACCATAA GGCAGAGGTT GAGTCCAACA 
TCCAATAAAA GTTCTCGCAA GTTATTTGAT CAAGCCAACA AGCTCAAAAA GACCTGTTCT 
CATCTTTTTA CAGCCACAAT CAACGTGAAT TCAGCCAACG ATGGCTGGTT TGGCAGCTTG 
AAGGACAGCA TTCAGCAGCA GCAAAACGAA GCAGTCTGGG TCTCTGAAGG AAAGATGGAA 
GGGATGGATG ATGATGCTGA AGACCGCATG TCCTACTTAA CCGCCATGGG CGCGGACTAT 
CTGAGTTGTG ACAGCCGTCT CATCAGTGAC TTTGAAGACA CCGACGGCGA GGGAGGCGCC 
TACACTGACA ATGAGCTAGA GGAGCCGGCG GAGGAGCCGC TGGTGTCTTC CATCACCCGC 
TCCTCGGAGC CGGTGCAGCA CGAGGAGGCA AGAAGCCAGA ACAGAGAAGA CTCCTTCGAC 
TACTCCAAGT CAAACCTCCC CGCCACAGCC GGCAGTGAAA TCCCGGGGGG ATCCACCAAA 
GGCTATCCTC CCCCTATTGC AGCGAAGCCT GCCTTTGGGC GGCCCATCCT GAAGCCTTCC 
ACTCCAGTCC CTATGCCTGA GAGCGAGGAG GTTGGAGAGA GCACCGAGGA GCAGGAAGAT 
GCTCCCAGAT CAGTCCTGGG CAGAGTGAAA ATATTCGAGA AGATGGACCA CAAGGCAAAA 
TTACAGAGGA TGCAGGAGCT CCAGGAGGCC CAGAATGCGA GGATCGAAAT AGCTCAGAAG 
CACCCTGACA TCTATGCGGT TCCAATCAAG GCCCCCAAGC CAGATGCTGG CCTCCCCCCG 
CACATGAGTT CTAGACCCCC AGAGCCCCAG AAAGCTCCTT CCAGACTTTA CCAGGACACC 
AGAGGAAGCT ACGGCAGTGA CCCCGAGGAG GAGGAGTACC GCCAGCAGTT GGCAGCACAC 
TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACGGAGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_017318137.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173626.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu79931
Clone ID Related Accession (Same CDS sequence)	XM_017318137.1 , NM_001360392.1 , XM_017318136.1
Accession Version	XM_017318137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173626.1
CDS	411..3803
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_017318137.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318137.1

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_017318136.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173625.1 Mus musculus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.

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CloneID	OMu79931
Clone ID Related Accession (Same CDS sequence)	XM_017318137.1 , NM_001360392.1 , XM_017318136.1
Accession Version	XM_017318136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173625.1
CDS	177..3569
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSRERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRSREHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPEERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQPRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGVKKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEEARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPILKPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_017318136.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318136.1

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA GCAGTACACC GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATTG CCGTGTCGGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATCT CAGATGTGCT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAAGATGTGC TCCATTCGTT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATAGCAGCC ATCGTGGTCA AGAGACCCCG GAAGGTTCAG 
GTGGCCCCAC TGCAAGGCAG CCCCCCTCTC AGTCATGACG ACCGAGGTTT TGAAGTGATT 
GAAGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGTCG GCACAGCAGC 
CACGACATGC TCAGCCACAG CTGGGAGGGC AATAGGGAGA GGGGGCGACC CCATCAGCGC 
ACACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCAGGAGCC TGGAACGGGG CCTGGACCAG 
GAAGACTATG GGCGCAGCCG TGAGCGCAGC CGTGGCCGGA GCCTGGAGCG CGGGCTGGAC 
CGCGACTTTG TGTCCAGGGA CCACAGCCGT GGCCGTAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CGAAGCTTAT GAACCCGACT ACGGAGGCGG CTACAGCCCA 
TCATATGACC GTAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGTTCCC GAAGCCCCAG CCCCGAGTCT AGGTCACGGC ATGAACACAA GGGCCAACAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTA ACCAAAAGCA AAGCAAATGA AGAGTATGGG 
CTCCGGCTGG GGAGTCAGAT CTTCATCAAA GAAATGACCA GAACAGGACT GGCCACCAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ACGGCACTGT TACTGAGAAC 
ATGTCTCTAA CGGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTTGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAG CCCTAAACGA CAGCGACTCT 
GAAGTGGAGG ATATCTCGGA AATAGAGTCC AACCGGTCTT TCTCTCCAGA GGAGAGACGC 
CAGCAGTATT CTGATCAGGA TTACCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCGAGC 
TCAAGAGAGG AGACCTCAGG CAGACTATCC AGGATGGGTG CCACTCCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCGGGGGTC ACAGAGGCCA GCAGGGAGCC CAGGTACCAG 
GAAGAAGGCC CAGTTCCTCA ACCCAGAACA GCTCCAAGAG TTTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA GGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAACGAC GTTGGGATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCCG CAGAGCAGGA GGGCCTGCAA GAAGGAGACC AGATTCTGAA GGTGAACACA 
CAGGATTTCA GAGGGCTAGT CCGGGAAGAT GCGGTCCTCT ACCTGCTAGA AATCCCCAAA 
GGTGAAACTG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTACAGAGA TATCCTGGCT 
TGTGGCAGGG GAGATTCGTT TTTCATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCC 
CAGAGCCTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAGCTGGGCC ACTGGCTGGC CGTGAGGATC GGGAATGAGC TGGAGAAAGG CTTGATCCCT 
AACAAAAGCA GAGCCGAGCA AATGGCCAGT GTCCAGAATG CCCAGCGAGA GAACGCCGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCA GCGGCGGCGT CAAGAAGAAC 
CTCCGCAAGA GCCGGGAAGA CCTGGCGGCT GCTGTATCGG TTAGCACCAA GTTCCCTGCC 
TACGAGAAGG TTCTGCTTCG GGAAGCTGGC TTCAAGAGAC CCGTGGTTCT GTTTGGCCCC 
ATAGCAGATA TAGCAATGGA AAGGTTGGCA ACTGAGTTAC CAGACCTGTT TCAAACTGCA 
AAAACAGAAC CCAAAGATGC CGGATCTGAG AAATCCAGTG GAGTGGTTCG GTTGAATACC 
GTGAGGCAAA TTATTGAGCA GGACAAGCAC GCCCTGCTCG ACGTTACCCC CAAAGCTGTG 
GACCTGCTCA ACTACACTCA GTGGTTCCCT ATCGTGATTT TCTTCAACCC GGATTCCAGA 
CAAGGTGTTA AAACCATAAG GCAGAGGTTG AGTCCAACAT CCAATAAAAG TTCTCGCAAG 
TTATTTGATC AAGCCAACAA GCTCAAAAAG ACCTGTTCTC ATCTTTTTAC AGCCACAATC 
AACGTGAATT CAGCCAACGA TGGCTGGTTT GGCAGCTTGA AGGACAGCAT TCAGCAGCAG 
CAAAACGAAG CAGTCTGGGT CTCTGAAGGA AAGATGGAAG GGATGGATGA TGATGCTGAA 
GACCGCATGT CCTACTTAAC CGCCATGGGC GCGGACTATC TGAGTTGTGA CAGCCGTCTC 
ATCAGTGACT TTGAAGACAC CGACGGCGAG GGAGGCGCCT ACACTGACAA TGAGCTAGAG 
GAGCCGGCGG AGGAGCCGCT GGTGTCTTCC ATCACCCGCT CCTCGGAGCC GGTGCAGCAC 
GAGGAGGCAA GAAGCCAGAA CAGAGAAGAC TCCTTCGACT ACTCCAAGTC AAACCTCCCC 
GCCACAGCCG GCAGTGAAAT CCCGGGGGGA TCCACCAAAG GCTATCCTCC CCCTATTGCA 
GCGAAGCCTG CCTTTGGGCG GCCCATCCTG AAGCCTTCCA CTCCAGTCCC TATGCCTGAG 
AGCGAGGAGG TTGGAGAGAG CACCGAGGAG CAGGAAGATG CTCCCAGATC AGTCCTGGGC 
AGAGTGAAAA TATTCGAGAA GATGGACCAC AAGGCAAAAT TACAGAGGAT GCAGGAGCTC 
CAGGAGGCCC AGAATGCGAG GATCGAAATA GCTCAGAAGC ACCCTGACAT CTATGCGGTT 
CCAATCAAGG CCCCCAAGCC AGATGCTGGC CTCCCCCCGC ACATGAGTTC TAGACCCCCA 
GAGCCCCAGA AAGCTCCTTC CAGACTTTAC CAGGACACCA GAGGAAGCTA CGGCAGTGAC 
CCCGAGGAGG AGGAGTACCG CCAGCAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC 
CAGCCCTCCC GGTACCGAGA CACGGAGTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Tjp2
Entrez Gene ID	21873
Full Name	tight junction protein 2
Synonyms	ZO-2
General protein information	Preferred Names tight junction protein 2 Names tight junction protein ZO-2 Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2) zona occludens protein 2 zonula occludens protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 B

Pan troglodytes (chimpanzee)	TJP2	XP_003312175.1
Danio rerio (zebrafish)	tjp2a	NP_001188499.1
Xenopus tropicalis (tropical clawed frog)	tjp2	XP_004910833.1
	LOC699586	XP_002808149.1
Canis lupus familiaris (dog)	TJP2	NP_001003204.1
Mus musculus (house mouse)	Tjp2	NP_001185914.1
Gallus gallus (chicken)	TJP2	NP_990249.1
Homo sapiens (human)	TJP2	NP_001163887.1
Rattus norvegicus (Norway rat)	Tjp2	NP_446225.1

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Tjp2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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