Marchf11 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Marchf11
Entrez Gene ID	211147
Full Name	membrane-associated ring finger (C3HC4) 11
Synonyms	9630025C22
General protein information	Preferred Names membrane-associated ring finger (C3HC4) 11 Names E3 ubiquitin-protein ligase MARCH11 MARCH-XI RING-type E3 ubiquitin transferase MARCH11 membrane-associated RING finger protein 11 membrane-associated RING-CH protein XI
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_177597.6	NP_808265.2	E3 ubiquitin-protein ligase MARCH11 isoform 1
NM_001310774.1	NP_001297703.1	E3 ubiquitin-protein ligase MARCH11 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MARCH11	XP_536522.2
Bos taurus (cattle)	MARCH11	NP_001192349.1
Mus musculus (house mouse)	March11	NP_808265.2
Rattus norvegicus (Norway rat)	March11	NP_001095298.1
Pan troglodytes (chimpanzee)	MARCH11	XP_003310679.1
Xenopus tropicalis (tropical clawed frog)	march11	XP_002933170.1
Homo sapiens (human)	MARCH11	NP_001096032.1
Danio rerio (zebrafish)	march11	XP_001338632.1
	LOC717920	XP_001109958.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)

Identification of SAMT family proteins as substrates of MARCH11 in mouse spermatids.
Yogo K, Tojima H, Ohno JY, Ogawa T, Nakamura N, Hirose S, Takeya T, Kohsaka T
Histochemistry and cell biology137(1)53-65(2012 Jan)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that SAMT1 proteins are physiological substrates of MARCH11 and are delivered to lysosomes through the TGN-MVB pathway by a ubiquitin-dependent sorting system in mouse spermatids
Title: Identification of SAMT family proteins as substrates of MARCH11 in mouse spermatids.

The following Marchf11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Marchf11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06504	NM_177597.6 Latest version!	Mus musculus membrane associated ring-CH-type finger 11 (Marchf11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu72749	NM_001310774.1 Latest version!	Mus musculus membrane associated ring-CH-type finger 11 (Marchf11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Marchf11 ( NM_177597.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808265.2 Mus musculus membrane associated ring-CH-type finger 11 (Marchf11), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06504
Clone ID Related Accession (Same CDS sequence)	NM_177597.6
Accession Version	NM_177597.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MARCH11 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK035990.1, BU961611.1, AK162044.1 and BF467885.2. On Jul 17, 2015 this sequence version replaced NM_177597.5. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BU961611.1, CA465889.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGACG AGGGCAAAAA ACGGTCTAGC CGGGCGGACA GCCTCGAAGC TGAGCCACCT 
CTGCCCCCGC CGCCCCCACC CCCGCCTCCG GGAGAGTCGG TCCTGGTCCC CACCTCCCCA 
CGCTACCGGC CGCCGCTGCC CGCGCCTCTG GAGCGCATCG TAGGGTCGGG CGAGCCGCCG 
GTAGAACTGG CCCCTCGGCG CAAGGGCGCA GGAGAGCCTC TACCGCCGCT GCCGCCGTCT 
CTGTTGCCCG GCGACCAGGA GGTGACAGCG GCGGGCGACT CCTGCGAAGG TCCTAGGCGC 
CTTCCAGAGG TGAAGCTCCC CGAGGCTGCG ACGGGGAAAG GCAGCCCGGG GGAGCCCGAG 
GCCGGCGCGT GCCGGGAGGG CGAGCGGCGA GGCACCGGAG ACCAGCCCGA AACACGCTCC 
GTATGTAGCA GCCGCAGCAG CAGTAGTGGC GGCGGTAGCG ACCAGCGCTC GGGGCACCAG 
CACCAGCACC ATCAGCCCAT TTGCAAGATC TGCTTCCAGG GCGCAGAGCA GGGTGAGTTG 
TTAAATCCTT GCCGATGTGA TGGGTCTGTT CGATACACAC ATCAGCTGTG TCTTCTAAAG 
TGGATCAGTG AGAGAGGCTC CTGGACCTGC GAGCTGTGCT GTTATAGATA CCACGTCACA 
GCCATTAAAA TGAAGCAACC TTGCCAGTGG CAGAGCATTT CTATAACACT GGTTGAGAAA 
GTTCAGATGA TCGCTGTAAT CCTAGGATCC CTGTTCTTAA TAGCGAGTGT GACTTGGCTC 
CTCTGGTCAG CCTTCAGCCC CTATGCAGTG TGGCAGAGGA AGGACATCCT TTTTCAAATC 
TGCTATGGAA TGTATGGTTT TATGGATCTA GTGTGCATAG GCCTCATTGT GCATGAAGGA 
GCTGCCGTTT ACAGAGTGTT TAAGCGATGG CGAGCTGTTA ATTTGCACTG GGATGTGTTA 
AATTATGACA AAGCCACAGA CATTGAAGAA AGCAGCCGAG GAGAGTCTTC CACAAGTAGG 
ACTTTGTGGC TACCATTGTC AGCTCTGAGG AACAGGAACT TGGTCCACCC AACGCAACTA 
ACCTCACCAC GGTTTCAGTG TGGCTATGTG CTATTACATC TATTCAATCG GATGAGGGCC 
CATGAAGATG TATCGGAAGA TAACGGCTCT GGGGAGGTAG TGATGAGAGT GACGTCTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808265.2
CDS	215..1417
Translation	MSDEGKKRSSRADSLEAEPPLPPPPPPPPPGESVLVPTSPRYRPPLPAPLERIVGSGEPPVELAPRRKGAGEPLPPLPPSLLPGDQEVTAAGDSCEGPRRLPEVKLPEAATGKGSPGEPEAGACREGERRGTGDQPETRSVCSSRSSSSGGGSDQRSGHQHQHHQPICKICFQGAEQGELLNPCRCDGSVRYTHQLCLLKWISERGSWTCELCCYRYHVTAIKMKQPCQWQSISITLVEKVQMIAVILGSLFLIASVTWLLWSAFSPYAVWQRKDILFQICYGMYGFMDLVCIGLIVHEGAAVYRVFKRWRAVNLHWDVLNYDKATDIEESSRGESSTSRTLWLPLSALRNRNLVHPTQLTSPRFQCGYVLLHLFNRMRAHEDVSEDNGSGEVVMRVTSV

Target ORF information:

RefSeq Version	NM_177597.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane associated ring-CH-type finger 11 (Marchf11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177597.6

ORF Insert Sequence:

ATGAGCGACG AGGGCAAAAA ACGGTCTAGC CGGGCGGACA GCCTCGAAGC TGAGCCACCT 
CTGCCCCCGC CGCCCCCACC CCCGCCTCCG GGAGAGTCGG TCCTGGTCCC CACCTCCCCA 
CGCTACCGGC CGCCGCTGCC CGCGCCTCTG GAGCGCATCG TAGGGTCGGG CGAGCCGCCG 
GTAGAACTGG CCCCTCGGCG CAAGGGCGCA GGAGAGCCTC TACCGCCGCT GCCGCCGTCT 
CTGTTGCCCG GCGACCAGGA GGTGACAGCG GCGGGCGACT CCTGCGAAGG TCCTAGGCGC 
CTTCCAGAGG TGAAGCTCCC CGAGGCTGCG ACGGGGAAAG GCAGCCCGGG GGAGCCCGAG 
GCCGGCGCGT GCCGGGAGGG CGAGCGGCGA GGCACCGGAG ACCAGCCCGA AACACGCTCC 
GTATGTAGCA GCCGCAGCAG CAGTAGTGGC GGCGGTAGCG ACCAGCGCTC GGGGCACCAG 
CACCAGCACC ATCAGCCCAT TTGCAAGATC TGCTTCCAGG GCGCAGAGCA GGGTGAGTTG 
TTAAATCCTT GCCGATGTGA TGGGTCTGTT CGATACACAC ATCAGCTGTG TCTTCTAAAG 
TGGATCAGTG AGAGAGGCTC CTGGACCTGC GAGCTGTGCT GTTATAGATA CCACGTCACA 
GCCATTAAAA TGAAGCAACC TTGCCAGTGG CAGAGCATTT CTATAACACT GGTTGAGAAA 
GTTCAGATGA TCGCTGTAAT CCTAGGATCC CTGTTCTTAA TAGCGAGTGT GACTTGGCTC 
CTCTGGTCAG CCTTCAGCCC CTATGCAGTG TGGCAGAGGA AGGACATCCT TTTTCAAATC 
TGCTATGGAA TGTATGGTTT TATGGATCTA GTGTGCATAG GCCTCATTGT GCATGAAGGA 
GCTGCCGTTT ACAGAGTGTT TAAGCGATGG CGAGCTGTTA ATTTGCACTG GGATGTGTTA 
AATTATGACA AAGCCACAGA CATTGAAGAA AGCAGCCGAG GAGAGTCTTC CACAAGTAGG 
ACTTTGTGGC TACCATTGTC AGCTCTGAGG AACAGGAACT TGGTCCACCC AACGCAACTA 
ACCTCACCAC GGTTTCAGTG TGGCTATGTG CTATTACATC TATTCAATCG GATGAGGGCC 
CATGAAGATG TATCGGAAGA TAACGGCTCT GGGGAGGTAG TGATGAGAGT GACGTCTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Marchf11 ( NM_001310774.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297703.1 Mus musculus membrane associated ring-CH-type finger 11 (Marchf11), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu72749
Clone ID Related Accession (Same CDS sequence)	NM_001310774.1
Accession Version	NM_001310774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MARCH11 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK035990.1, AK162044.1 and BF467885.2. On Jul 17, 2015 this sequence version replaced XM_006520045.2. Transcript Variant: This variant (2) lacks an exon, which results in a frameshift compared to variant 1. The resulting isoform (2) is shorter with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK162044.1, SRR1660819.384588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGACG AGGGCAAAAA ACGGTCTAGC CGGGCGGACA GCCTCGAAGC TGAGCCACCT 
CTGCCCCCGC CGCCCCCACC CCCGCCTCCG GGAGAGTCGG TCCTGGTCCC CACCTCCCCA 
CGCTACCGGC CGCCGCTGCC CGCGCCTCTG GAGCGCATCG TAGGGTCGGG CGAGCCGCCG 
GTAGAACTGG CCCCTCGGCG CAAGGGCGCA GGAGAGCCTC TACCGCCGCT GCCGCCGTCT 
CTGTTGCCCG GCGACCAGGA GGTGACAGCG GCGGGCGACT CCTGCGAAGG TCCTAGGCGC 
CTTCCAGAGG TGAAGCTCCC CGAGGCTGCG ACGGGGAAAG GCAGCCCGGG GGAGCCCGAG 
GCCGGCGCGT GCCGGGAGGG CGAGCGGCGA GGCACCGGAG ACCAGCCCGA AACACGCTCC 
GTATGTAGCA GCCGCAGCAG CAGTAGTGGC GGCGGTAGCG ACCAGCGCTC GGGGCACCAG 
CACCAGCACC ATCAGCCCAT TTGCAAGATC TGCTTCCAGG GCGCAGAGCA GGGTGAGTTG 
TTAAATCCTT GCCGATGTGA TGGGTCTGTT CGATACACAC ATCAGCTGTG TCTTCTAAAG 
TGGATCAGTG AGAGAGGCTC CTGGACCTGC GAGCTGTGCT GTTATAGATA CCACGTCACA 
GCCATTAAAA TGAAGCAACC TTGCCAGGCC TCATTGTGCA TGAAGGAGCT GCCGTTTACA 
GAGTGTTTAA GCGATGGCGA GCTGTTAATT TGCACTGGGA TGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297703.1
CDS	215..982
Translation	MSDEGKKRSSRADSLEAEPPLPPPPPPPPPGESVLVPTSPRYRPPLPAPLERIVGSGEPPVELAPRRKGAGEPLPPLPPSLLPGDQEVTAAGDSCEGPRRLPEVKLPEAATGKGSPGEPEAGACREGERRGTGDQPETRSVCSSRSSSSGGGSDQRSGHQHQHHQPICKICFQGAEQGELLNPCRCDGSVRYTHQLCLLKWISERGSWTCELCCYRYHVTAIKMKQPCQASLCMKELPFTECLSDGELLICTGMC

Target ORF information:

RefSeq Version	NM_001310774.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane associated ring-CH-type finger 11 (Marchf11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310774.1

ORF Insert Sequence:

ATGAGCGACG AGGGCAAAAA ACGGTCTAGC CGGGCGGACA GCCTCGAAGC TGAGCCACCT 
CTGCCCCCGC CGCCCCCACC CCCGCCTCCG GGAGAGTCGG TCCTGGTCCC CACCTCCCCA 
CGCTACCGGC CGCCGCTGCC CGCGCCTCTG GAGCGCATCG TAGGGTCGGG CGAGCCGCCG 
GTAGAACTGG CCCCTCGGCG CAAGGGCGCA GGAGAGCCTC TACCGCCGCT GCCGCCGTCT 
CTGTTGCCCG GCGACCAGGA GGTGACAGCG GCGGGCGACT CCTGCGAAGG TCCTAGGCGC 
CTTCCAGAGG TGAAGCTCCC CGAGGCTGCG ACGGGGAAAG GCAGCCCGGG GGAGCCCGAG 
GCCGGCGCGT GCCGGGAGGG CGAGCGGCGA GGCACCGGAG ACCAGCCCGA AACACGCTCC 
GTATGTAGCA GCCGCAGCAG CAGTAGTGGC GGCGGTAGCG ACCAGCGCTC GGGGCACCAG 
CACCAGCACC ATCAGCCCAT TTGCAAGATC TGCTTCCAGG GCGCAGAGCA GGGTGAGTTG 
TTAAATCCTT GCCGATGTGA TGGGTCTGTT CGATACACAC ATCAGCTGTG TCTTCTAAAG 
TGGATCAGTG AGAGAGGCTC CTGGACCTGC GAGCTGTGCT GTTATAGATA CCACGTCACA 
GCCATTAAAA TGAAGCAACC TTGCCAGGCC TCATTGTGCA TGAAGGAGCT GCCGTTTACA 
GAGTGTTTAA GCGATGGCGA GCTGTTAATT TGCACTGGGA TGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)
Miyata H,Castaneda JM,Fujihara Y,Yu Z,Archambeault DR,Isotani A,Kiyozumi D,Kriseman ML,Mashiko D,Matsumura T,Matzuk RM,Mori M,Noda T,Oji A,Okabe M,Prunskaite-Hyyrylainen R,Ramirez-Solis R,Satouh Y,Zhang Q,Ikawa M,Matzuk MM

Identification of SAMT family proteins as substrates of MARCH11 in mouse spermatids.
Histochemistry and cell biology137(1)53-65(2012 Jan)
Yogo K,Tojima H,Ohno JY,Ogawa T,Nakamura N,Hirose S,Takeya T,Kohsaka T

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Marchf11 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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