Rpl21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rpl21
Entrez Gene ID	19933
Full Name	ribosomal protein L21
Synonyms	8430440E03Rik,L21
General protein information	Preferred Names ribosomal protein L21 Names 60S ribosomal protein L21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320746.2	XP_017176235.1	60S ribosomal protein L21 isoform X1
XM_017320747.2	XP_017176236.1	60S ribosomal protein L21 isoform X1
NM_019647.6	NP_062621.2	60S ribosomal protein L21
XM_017320746.1	XP_017176235.1	60S ribosomal protein L21 isoform X1
XM_017320747.1	XP_017176236.1	60S ribosomal protein L21 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RPL21	NP_000973.2
Bos taurus (cattle)	RPL21	NP_001178341.1
Rattus norvegicus (Norway rat)	Rpl21	NP_445782.1
Caenorhabditis elegans (roundworm)	rpl-21	NP_498774.1
Saccharomyces cerevisiae (baker's yeast)	RPL21B	NP_015246.1
Magnaporthe oryzae (rice blast fungus)	MGG_02953	XP_003720754.1
	LOC703876	XP_001094881.1
Gallus gallus (chicken)	RPL21	XP_001233393.1
Schizosaccharomyces pombe (fission yeast)	rpl2102	NP_594175.1
Mus musculus (house mouse)	Rpl21	NP_062621.2
Drosophila melanogaster (fruit fly)	RpL21	NP_001260689.1
Kluyveromyces lactis	KLLA0E23651g	XP_455019.1
Neurospora crassa	NCU01948	XP_964232.1
Arabidopsis thaliana (thale cress)	AT1G09590	NP_563847.1
Arabidopsis thaliana (thale cress)	AT1G09690	NP_563849.1
Oryza sativa (rice)	Os10g0465800	NP_001064799.1
Xenopus tropicalis (tropical clawed frog)	rpl21	NP_001005026.1
Canis lupus familiaris (dog)	RPL21	NP_001238973.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012138	XP_320389.2
Arabidopsis thaliana (thale cress)	AT1G57660	NP_564724.1
Danio rerio (zebrafish)	rpl21	NP_001002155.1
Saccharomyces cerevisiae (baker's yeast)	RPL21A	NP_009750.1
Eremothecium gossypii	AGOS_AEL254W	NP_984607.1
Schizosaccharomyces pombe (fission yeast)	rpl2101	NP_596032.1
Arabidopsis thaliana (thale cress)	AT1G57860	NP_564726.1
Oryza sativa (rice)	Os03g0141000	NP_001173260.1

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of the sclerotome-enriched lncRNA PEAT augments ribosomal protein expression.
Stafford DA, Dichmann DS, Chang JK, Harland RM
Proceedings of the National Academy of Sciences of the United States of America114(1)101-106(2017 Jan)

Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication.
Salinas E, Byrum SD, Moreland LE, Mackintosh SG, Tackett AJ, Forrest JC
Journal of virology90(3)1397-413(2015 Nov)

Blastocyst implantation failure relates to impaired translational machinery gene expression.
Plaks V, Gershon E, Zeisel A, Jacob-Hirsch J, Neeman M, Winterhager E, Rechavi G, Domany E, Dekel N
Reproduction (Cambridge, England)148(1)87-98(2014 Jul)

Ribosome-mediated specificity in Hox mRNA translation and vertebrate tissue patterning.
Kondrashov N, Pusic A, Stumpf CR, Shimizu K, Hsieh AC, Ishijima J, Shiroishi T, Barna M
Cell145(3)383-397(2011 Apr)

Global analysis of gene expression in the developing brain of Gtf2ird1 knockout mice.
O'Leary J, Osborne LR
PloS one6(8)e23868(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast.
Title: In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar.

The following Rpl21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rpl21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11522	NM_019647.6 Latest version!	Mus musculus ribosomal protein L21 (Rpl21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu81283	XM_017320747.2 Latest version!	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu81283	XM_017320746.2 Latest version!	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu81283	XM_017320746.1	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu81283	XM_017320747.1	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rpl21 ( NM_019647.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062621.2 Mus musculus ribosomal protein L21 (Rpl21), mRNA.

Price & Availability↑

CloneID	OMu11522
Clone ID Related Accession (Same CDS sequence)	NM_019647.6
Accession Version	NM_019647.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	60S ribosomal protein L21
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY264537.1, AK008457.1, AK018480.1 and BQ031254.1. On Jul 31, 2009 this sequence version replaced NM_019647.5. ##Evidence-Data-START## Transcript exon combination :: AK018480.1, BF608887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGACGAACA CAAAGGGAAA GAGGAGAGGC ACCCGGTACA TGTTCTCTAG GCCTTTTAGG 
AAACATGGCG TTGTTCCTTT GGCCACATAC ATGCGAATCT ACAAGAAGGG TGATATTGTA 
GACATCAAGG GAATGGGCAC TGTTCAAAAA GGAATGCCCC ATAAGTGCTA CCACGGCAAA 
ACCGGAAGAG TCTACAATGT CACCCAGCAT GCCGTGGGCA TCATTGTCAA CAAGCAGGTT 
AAGGGCAAAA TTCTGGCCAA GAGGATCAAT GTGCGGATTG AGCACATCAA GCACTCAAAG 
AGCAGAGACA GCTTCCTGAA GCGGGTGAAG GAGAATGACC AGAAGAAAAA GGAAGCCAAA 
GAGAAGGGCA CCTGGGTGCA GCTGAAGCGC CAGCCTGCGC CACCCAGAGA AGCACACTTT 
GTGAGGACTA ATGGAAAAGA GCCTGAGCTG TTGGAGCCCA TTCCATACGA ATTCATGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062621.2
CDS	295..777
Translation	MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Target ORF information:

RefSeq Version	NM_019647.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribosomal protein L21 (Rpl21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019647.6

ORF Insert Sequence:

ATGACGAACA CAAAGGGAAA GAGGAGAGGC ACCCGGTACA TGTTCTCTAG GCCTTTTAGG 
AAACATGGCG TTGTTCCTTT GGCCACATAC ATGCGAATCT ACAAGAAGGG TGATATTGTA 
GACATCAAGG GAATGGGCAC TGTTCAAAAA GGAATGCCCC ATAAGTGCTA CCACGGCAAA 
ACCGGAAGAG TCTACAATGT CACCCAGCAT GCCGTGGGCA TCATTGTCAA CAAGCAGGTT 
AAGGGCAAAA TTCTGGCCAA GAGGATCAAT GTGCGGATTG AGCACATCAA GCACTCAAAG 
AGCAGAGACA GCTTCCTGAA GCGGGTGAAG GAGAATGACC AGAAGAAAAA GGAAGCCAAA 
GAGAAGGGCA CCTGGGTGCA GCTGAAGCGC CAGCCTGCGC CACCCAGAGA AGCACACTTT 
GTGAGGACTA ATGGAAAAGA GCCTGAGCTG TTGGAGCCCA TTCCATACGA ATTCATGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rpl21 ( XM_017320746.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176235.1 Mus musculus ribosomal protein L21 (Rpl21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81283
Clone ID Related Accession (Same CDS sequence)	XM_017320746.2 , XM_017320747.2 , XM_017320746.1 , XM_017320747.1
Accession Version	XM_017320746.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	60S ribosomal protein L21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176235.1
CDS	1178..1528
Translation	MGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Target ORF information:

RefSeq Version	XM_017320746.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320746.1

ORF Insert Sequence:

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rpl21 ( XM_017320747.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176236.1 Mus musculus ribosomal protein L21 (Rpl21), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81283
Clone ID Related Accession (Same CDS sequence)	XM_017320746.2 , XM_017320747.2 , XM_017320746.1 , XM_017320747.1
Accession Version	XM_017320747.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	60S ribosomal protein L21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176236.1
CDS	1004..1354
Translation	MGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Target ORF information:

RefSeq Version	XM_017320747.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320747.1

ORF Insert Sequence:

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rpl21 ( XM_017320746.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176235.1 Mus musculus ribosomal protein L21 (Rpl21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81283
Clone ID Related Accession (Same CDS sequence)	XM_017320746.2 , XM_017320747.2 , XM_017320746.1 , XM_017320747.1
Accession Version	XM_017320746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	60S ribosomal protein L21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176235.1
CDS	1192..1542
Translation	MGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Target ORF information:

RefSeq Version	XM_017320746.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320746.2

ORF Insert Sequence:

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rpl21 ( XM_017320747.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176236.1 Mus musculus ribosomal protein L21 (Rpl21), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81283
Clone ID Related Accession (Same CDS sequence)	XM_017320746.2 , XM_017320747.2 , XM_017320746.1 , XM_017320747.1
Accession Version	XM_017320747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	60S ribosomal protein L21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176236.1
CDS	1029..1379
Translation	MGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Target ORF information:

RefSeq Version	XM_017320747.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribosomal protein L21 (Rpl21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320747.2

ORF Insert Sequence:

ATGGGCACTG TTCAAAAAGG AATGCCCCAT AAGTGCTACC ACGGCAAAAC CGGAAGAGTC 
TACAATGTCA CCCAGCATGC CGTGGGCATC ATTGTCAACA AGCAGGTTAA GGGCAAAATT 
CTGGCCAAGA GGATCAATGT GCGGATTGAG CACATCAAGC ACTCAAAGAG CAGAGACAGC 
TTCCTGAAGC GGGTGAAGGA GAATGACCAG AAGAAAAAGG AAGCCAAAGA GAAGGGCACC 
TGGGTGCAGC TGAAGCGCCA GCCTGCGCCA CCCAGAGAAG CACACTTTGT GAGGACTAAT 
GGAAAAGAGC CTGAGCTGTT GGAGCCCATT CCATACGAAT TCATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of the sclerotome-enriched lncRNA PEAT augments ribosomal protein expression.
Proceedings of the National Academy of Sciences of the United States of America114(1)101-106(2017 Jan)
Stafford DA,Dichmann DS,Chang JK,Harland RM

Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication.
Journal of virology90(3)1397-413(2015 Nov)
Salinas E,Byrum SD,Moreland LE,Mackintosh SG,Tackett AJ,Forrest JC

Blastocyst implantation failure relates to impaired translational machinery gene expression.
Reproduction (Cambridge, England)148(1)87-98(2014 Jul)
Plaks V,Gershon E,Zeisel A,Jacob-Hirsch J,Neeman M,Winterhager E,Rechavi G,Domany E,Dekel N

Ribosome-mediated specificity in Hox mRNA translation and vertebrate tissue patterning.
Cell145(3)383-397(2011 Apr)
Kondrashov N,Pusic A,Stumpf CR,Shimizu K,Hsieh AC,Ishijima J,Shiroishi T,Barna M

Global analysis of gene expression in the developing brain of Gtf2ird1 knockout mice.
PloS one6(8)e23868(2011)
O'Leary J,Osborne LR

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Rpl21 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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