Rad1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rad1
Entrez Gene ID	19355
Full Name	RAD1 checkpoint DNA exonuclease
Synonyms	mRAD1
General protein information	Preferred Names RAD1 checkpoint DNA exonuclease Names cell cycle checkpoint protein RAD1 DNA repair exonuclease rad1 homolog RAD1 homolog rad1-like DNA damage checkpoint protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_011232.3	NP_035362.2	cell cycle checkpoint protein RAD1 isoform 1
NM_001289447.1	NP_001276376.1	cell cycle checkpoint protein RAD1 isoform 1
NM_001289448.1	NP_001276377.1	cell cycle checkpoint protein RAD1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAD1	NP_002844.1
Gallus gallus (chicken)	RAD1	XP_004937237.1
Magnaporthe oryzae (rice blast fungus)	MGG_17052	XP_003716208.1
Pan troglodytes (chimpanzee)	RAD1	XP_517813.2
Drosophila melanogaster (fruit fly)	Rad1	NP_477440.1
Xenopus tropicalis (tropical clawed frog)	rad1	NP_001016045.1
Canis lupus familiaris (dog)	RAD1	XP_005619459.1
Mus musculus (house mouse)	Rad1	NP_035362.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002255	XP_307928.5
Neurospora crassa	NCU00942	XP_964768.2
	RAD1	XP_001090119.1
Rattus norvegicus (Norway rat)	Rad1	NP_001099889.1
Danio rerio (zebrafish)	rad1	NP_957192.1
Schizosaccharomyces pombe (fission yeast)	rad1	NP_594809.1
Bos taurus (cattle)	RAD1	NP_001179419.1
Caenorhabditis elegans (roundworm)	mrt-2	NP_499521.1

Gene Ontology
Bibliography

Related articles in PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance.
Lyndaker AM, Lim PX, Mleczko JM, Diggins CE, Holloway JK, Holmes RJ, Kan R, Schlafer DH, Freire R, Cohen PE, Weiss RS
PLoS genetics9(2)e1003320(2013)

The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates.
Refolio E, Cavero S, Marcon E, Freire R, San-Segundo PA
Journal of cell science124(Pt 14)2488-500(2011 Jul)

Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses.
Zhang C, Liu Y, Hu Z, An L, He Y, Hang H
Protein & cell2(5)410-22(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HUS1 acts as a component of the canonical 9-1-1 complex during meiotic prophase I to promote DSB repair and further propose that RAD1 and TOPBP1 respond to unsynapsed chromatin through an alternative mechanism that does not require RAD9 or HUS1.
Title: Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance.

The rad1 protein is required for repairing DNA lesions induced by UV-light, HU and gamma rays, and for mediating G(2)/M and S/M checkpoint controls.
Title: Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses.

data indicate that Rad1(K185) is not a functional counterpart of PCNA(K164).
Title: Lysine residue 185 of Rad1 is a topological but not a functional counterpart of lysine residue 164 of PCNA.

data suggest that Mrad1 is important for preventing tumor development.
Title: Mouse Rad1 deletion enhances susceptibility for skin tumor development.

The following Rad1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rad1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17230	NM_001289448.1 Latest version!	Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu50667	NM_011232.3 Latest version!	Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu50667	NM_001289447.1 Latest version!	Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rad1 ( NM_001289448.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276377.1 Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu17230
Clone ID Related Accession (Same CDS sequence)	NM_001289448.1
Accession Version	NM_001289448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	cell cycle checkpoint protein RAD1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102101.7 and AK168588.1. On Jan 22, 2014 this sequence version replaced XM_006520040.1. Transcript Variant: This variant (3) differs in the 3' UTR and 3' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCTCC TAACCCAGTA CAATGAAGAG GAGTACGAAC AGTACTGCTT AGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTGTC TTGAAAGCCA TTCATTTCAG AGAACACGCC 
ACGTGTTTTG CTACCAAAAA CGGAATCAAG GTTACAGTGG AGAATGCAAA GTGTGTGCAA 
GCAAATGCCT TTATTCAGGC TGACGTGTTT CAGGAATTTG TCATTCAGGA AGAATCTGTT 
ACTTTTCGAA TTAACTTAAC TATCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ACACCAGGGA CTTTGACTGC GCTTCGGATG TGTTACCAAG GTTATGGTCA CCCACTGATG 
CTATTTCTAG AAGAAGGAGG AGTGGTGACG GTCTGCAAAA TTACCACTCA GGAGCCTGAG 
GAGACACTGG ATTTTGATTT CTGCAGCACC AATGTTATGA ATAAAATTAT CCTGCAGTCA 
GAGGGGCTCC GGGAAGCCTT TTCTGAGCTG GACATGACAG GTGATGTCCT ACAGATCACT 
GTGTCTCCTG ACAAGCCCTA TTTCAGGTTG TCTACTTTTG GAAATGCAGG AAACTCCCAT 
CTTGACTATC CCAAAGATTC CGACTTGGTG GAAGCCTTTC ACTGTGATAA GACCCAGGTC 
AACAGAATTT CGGATGTAAA GTATTCCTGT AAAACATGGT TGCAGCTACC TGAAGGGGTA 
GGCTATGATC CTCTCAGCGG TTTGGAGTGG ACTCTTTCAG AACGCGATGG AGAGCAGGAG 
GTGGCTCAGT GGGTAAACGT GGTCCCTGTG CAAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276377.1
CDS	314..1132
Translation	MPLLTQYNEEEYEQYCLVASLDNVRNLSTVLKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQADVFQEFVIQEESVTFRINLTILLDCLSIFGSSPTPGTLTALRMCYQGYGHPLMLFLEEGGVVTVCKITTQEPEETLDFDFCSTNVMNKIILQSEGLREAFSELDMTGDVLQITVSPDKPYFRLSTFGNAGNSHLDYPKDSDLVEAFHCDKTQVNRISDVKYSCKTWLQLPEGVGYDPLSGLEWTLSERDGEQEVAQWVNVVPVQA

Target ORF information:

RefSeq Version	NM_001289448.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289448.1

ORF Insert Sequence:

ATGCCTCTCC TAACCCAGTA CAATGAAGAG GAGTACGAAC AGTACTGCTT AGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTGTC TTGAAAGCCA TTCATTTCAG AGAACACGCC 
ACGTGTTTTG CTACCAAAAA CGGAATCAAG GTTACAGTGG AGAATGCAAA GTGTGTGCAA 
GCAAATGCCT TTATTCAGGC TGACGTGTTT CAGGAATTTG TCATTCAGGA AGAATCTGTT 
ACTTTTCGAA TTAACTTAAC TATCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ACACCAGGGA CTTTGACTGC GCTTCGGATG TGTTACCAAG GTTATGGTCA CCCACTGATG 
CTATTTCTAG AAGAAGGAGG AGTGGTGACG GTCTGCAAAA TTACCACTCA GGAGCCTGAG 
GAGACACTGG ATTTTGATTT CTGCAGCACC AATGTTATGA ATAAAATTAT CCTGCAGTCA 
GAGGGGCTCC GGGAAGCCTT TTCTGAGCTG GACATGACAG GTGATGTCCT ACAGATCACT 
GTGTCTCCTG ACAAGCCCTA TTTCAGGTTG TCTACTTTTG GAAATGCAGG AAACTCCCAT 
CTTGACTATC CCAAAGATTC CGACTTGGTG GAAGCCTTTC ACTGTGATAA GACCCAGGTC 
AACAGAATTT CGGATGTAAA GTATTCCTGT AAAACATGGT TGCAGCTACC TGAAGGGGTA 
GGCTATGATC CTCTCAGCGG TTTGGAGTGG ACTCTTTCAG AACGCGATGG AGAGCAGGAG 
GTGGCTCAGT GGGTAAACGT GGTCCCTGTG CAAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad1 ( NM_011232.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035362.2 Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu50667
Clone ID Related Accession (Same CDS sequence)	NM_011232.3 , NM_001289447.1
Accession Version	NM_011232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	cell cycle checkpoint protein RAD1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102101.7 and AK150466.1. On Jan 22, 2014 this sequence version replaced NM_011232.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 2 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146533.1, AK148098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCTCC TAACCCAGTA CAATGAAGAG GAGTACGAAC AGTACTGCTT AGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTGTC TTGAAAGCCA TTCATTTCAG AGAACACGCC 
ACGTGTTTTG CTACCAAAAA CGGAATCAAG GTTACAGTGG AGAATGCAAA GTGTGTGCAA 
GCAAATGCCT TTATTCAGGC TGACGTGTTT CAGGAATTTG TCATTCAGGA AGAATCTGTT 
ACTTTTCGAA TTAACTTAAC TATCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ACACCAGGGA CTTTGACTGC GCTTCGGATG TGTTACCAAG GTTATGGTCA CCCACTGATG 
CTATTTCTAG AAGAAGGAGG AGTGGTGACG GTCTGCAAAA TTACCACTCA GGAGCCTGAG 
GAGACACTGG ATTTTGATTT CTGCAGCACC AATGTTATGA ATAAAATTAT CCTGCAGTCA 
GAGGGGCTCC GGGAAGCCTT TTCTGAGCTG GACATGACAG GTGATGTCCT ACAGATCACT 
GTGTCTCCTG ACAAGCCCTA TTTCAGGTTG TCTACTTTTG GAAATGCAGG AAACTCCCAT 
CTTGACTATC CCAAAGATTC CGACTTGGTG GAAGCCTTTC ACTGTGATAA GACCCAGGTC 
AACAGATACA AGCTGTCGCT ACTGAAGCCC TCTACAAAGG CACTAGCTTT ATCCTGTAAA 
GTGTCTATCC GGACAGATAA CCGAGGCTTC CTCTCCTTAC AGTACATGAT TAGAAATGAA 
GATGGGCAGA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035362.2
CDS	314..1156
Translation	MPLLTQYNEEEYEQYCLVASLDNVRNLSTVLKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQADVFQEFVIQEESVTFRINLTILLDCLSIFGSSPTPGTLTALRMCYQGYGHPLMLFLEEGGVVTVCKITTQEPEETLDFDFCSTNVMNKIILQSEGLREAFSELDMTGDVLQITVSPDKPYFRLSTFGNAGNSHLDYPKDSDLVEAFHCDKTQVNRYKLSLLKPSTKALALSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPES

Target ORF information:

RefSeq Version	NM_011232.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011232.3

ORF Insert Sequence:

ATGCCTCTCC TAACCCAGTA CAATGAAGAG GAGTACGAAC AGTACTGCTT AGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTGTC TTGAAAGCCA TTCATTTCAG AGAACACGCC 
ACGTGTTTTG CTACCAAAAA CGGAATCAAG GTTACAGTGG AGAATGCAAA GTGTGTGCAA 
GCAAATGCCT TTATTCAGGC TGACGTGTTT CAGGAATTTG TCATTCAGGA AGAATCTGTT 
ACTTTTCGAA TTAACTTAAC TATCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ACACCAGGGA CTTTGACTGC GCTTCGGATG TGTTACCAAG GTTATGGTCA CCCACTGATG 
CTATTTCTAG AAGAAGGAGG AGTGGTGACG GTCTGCAAAA TTACCACTCA GGAGCCTGAG 
GAGACACTGG ATTTTGATTT CTGCAGCACC AATGTTATGA ATAAAATTAT CCTGCAGTCA 
GAGGGGCTCC GGGAAGCCTT TTCTGAGCTG GACATGACAG GTGATGTCCT ACAGATCACT 
GTGTCTCCTG ACAAGCCCTA TTTCAGGTTG TCTACTTTTG GAAATGCAGG AAACTCCCAT 
CTTGACTATC CCAAAGATTC CGACTTGGTG GAAGCCTTTC ACTGTGATAA GACCCAGGTC 
AACAGATACA AGCTGTCGCT ACTGAAGCCC TCTACAAAGG CACTAGCTTT ATCCTGTAAA 
GTGTCTATCC GGACAGATAA CCGAGGCTTC CTCTCCTTAC AGTACATGAT TAGAAATGAA 
GATGGGCAGA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad1 ( NM_001289447.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276376.1 Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu50667
Clone ID Related Accession (Same CDS sequence)	NM_011232.3 , NM_001289447.1
Accession Version	NM_001289447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	cell cycle checkpoint protein RAD1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102101.7. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048693.1, BU513151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCTCC TAACCCAGTA CAATGAAGAG GAGTACGAAC AGTACTGCTT AGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTGTC TTGAAAGCCA TTCATTTCAG AGAACACGCC 
ACGTGTTTTG CTACCAAAAA CGGAATCAAG GTTACAGTGG AGAATGCAAA GTGTGTGCAA 
GCAAATGCCT TTATTCAGGC TGACGTGTTT CAGGAATTTG TCATTCAGGA AGAATCTGTT 
ACTTTTCGAA TTAACTTAAC TATCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ACACCAGGGA CTTTGACTGC GCTTCGGATG TGTTACCAAG GTTATGGTCA CCCACTGATG 
CTATTTCTAG AAGAAGGAGG AGTGGTGACG GTCTGCAAAA TTACCACTCA GGAGCCTGAG 
GAGACACTGG ATTTTGATTT CTGCAGCACC AATGTTATGA ATAAAATTAT CCTGCAGTCA 
GAGGGGCTCC GGGAAGCCTT TTCTGAGCTG GACATGACAG GTGATGTCCT ACAGATCACT 
GTGTCTCCTG ACAAGCCCTA TTTCAGGTTG TCTACTTTTG GAAATGCAGG AAACTCCCAT 
CTTGACTATC CCAAAGATTC CGACTTGGTG GAAGCCTTTC ACTGTGATAA GACCCAGGTC 
AACAGATACA AGCTGTCGCT ACTGAAGCCC TCTACAAAGG CACTAGCTTT ATCCTGTAAA 
GTGTCTATCC GGACAGATAA CCGAGGCTTC CTCTCCTTAC AGTACATGAT TAGAAATGAA 
GATGGGCAGA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276376.1
CDS	102..944
Translation	MPLLTQYNEEEYEQYCLVASLDNVRNLSTVLKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQADVFQEFVIQEESVTFRINLTILLDCLSIFGSSPTPGTLTALRMCYQGYGHPLMLFLEEGGVVTVCKITTQEPEETLDFDFCSTNVMNKIILQSEGLREAFSELDMTGDVLQITVSPDKPYFRLSTFGNAGNSHLDYPKDSDLVEAFHCDKTQVNRYKLSLLKPSTKALALSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPES

Target ORF information:

RefSeq Version	NM_001289447.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD1 checkpoint DNA exonuclease (Rad1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289447.1

ORF Insert Sequence:

ATGCCTCTCC TAACCCAGTA CAATGAAGAG GAGTACGAAC AGTACTGCTT AGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTGTC TTGAAAGCCA TTCATTTCAG AGAACACGCC 
ACGTGTTTTG CTACCAAAAA CGGAATCAAG GTTACAGTGG AGAATGCAAA GTGTGTGCAA 
GCAAATGCCT TTATTCAGGC TGACGTGTTT CAGGAATTTG TCATTCAGGA AGAATCTGTT 
ACTTTTCGAA TTAACTTAAC TATCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ACACCAGGGA CTTTGACTGC GCTTCGGATG TGTTACCAAG GTTATGGTCA CCCACTGATG 
CTATTTCTAG AAGAAGGAGG AGTGGTGACG GTCTGCAAAA TTACCACTCA GGAGCCTGAG 
GAGACACTGG ATTTTGATTT CTGCAGCACC AATGTTATGA ATAAAATTAT CCTGCAGTCA 
GAGGGGCTCC GGGAAGCCTT TTCTGAGCTG GACATGACAG GTGATGTCCT ACAGATCACT 
GTGTCTCCTG ACAAGCCCTA TTTCAGGTTG TCTACTTTTG GAAATGCAGG AAACTCCCAT 
CTTGACTATC CCAAAGATTC CGACTTGGTG GAAGCCTTTC ACTGTGATAA GACCCAGGTC 
AACAGATACA AGCTGTCGCT ACTGAAGCCC TCTACAAAGG CACTAGCTTT ATCCTGTAAA 
GTGTCTATCC GGACAGATAA CCGAGGCTTC CTCTCCTTAC AGTACATGAT TAGAAATGAA 
GATGGGCAGA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance.
PLoS genetics9(2)e1003320(2013)
Lyndaker AM,Lim PX,Mleczko JM,Diggins CE,Holloway JK,Holmes RJ,Kan R,Schlafer DH,Freire R,Cohen PE,Weiss RS

The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates.
Journal of cell science124(Pt 14)2488-500(2011 Jul)
Refolio E,Cavero S,Marcon E,Freire R,San-Segundo PA

Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses.
Protein & cell2(5)410-22(2011 May)
Zhang C,Liu Y,Hu Z,An L,He Y,Hang H