Rab18 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab18
Entrez Gene ID	19330
Full Name	RAB18, member RAS oncogene family
Synonyms	AA959686
General protein information	Preferred Names RAB18, member RAS oncogene family Names ras-related protein Rab-18 GTP-binding protein Rab18
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 A1\|18 4.53 cM
Summary	This gene encodes a member of the Ras-related small GTPases, which regulate membrane trafficking in organelles and transport vesicles. This protein is expressed predominantly in lipid droplets, organelles that store neutral lipids, and is proposed to play a role in lipolysis and lipogenesis. In humans mutations in this gene are associated with Warburg micro syndrome type 3. A pseudogene of this gene is located on chromosome X. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_181070.6	NP_851415.1	ras-related protein Rab-18 isoform 2 precursor
NM_001278447.1	NP_001265376.1	ras-related protein Rab-18 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RAB18	XP_005617019.1
Bos taurus (cattle)	RAB18	NP_001068967.1
Rattus norvegicus (Norway rat)	Rab18	NP_001012486.1
Arabidopsis thaliana (thale cress)	RAB18	NP_175056.1
Drosophila melanogaster (fruit fly)	Rab18	NP_524744.2
Mus musculus (house mouse)	Rab18	NP_001265376.1
Gallus gallus (chicken)	RAB18	NP_001006355.1
Danio rerio (zebrafish)	rab18b	NP_001003449.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010875	XP_309827.4
Caenorhabditis elegans (roundworm)	rab-18	NP_741092.1
Arabidopsis thaliana (thale cress)	RABC2b	NP_187602.1
Xenopus tropicalis (tropical clawed frog)	rab18	NP_001017281.1
Homo sapiens (human)	RAB18	NP_001243339.1
Pan troglodytes (chimpanzee)	RAB18	XP_507716.2
	RAB18	XP_001104913.1
Arabidopsis thaliana (thale cress)	RABC2A	NP_568121.1

Gene Ontology
Bibliography

Related articles in PubMed

RAB18, a protein associated with Warburg Micro syndrome, controls neuronal migration in the developing cerebral cortex.
Wu Q, Sun X, Yue W, Lu T, Ruan Y, Chen T, Zhang D
Molecular brain919(2016 Feb)

Regulated localization of Rab18 to lipid droplets: effects of lipolytic stimulation and inhibition of lipid droplet catabolism.
Martin S, Driessen K, Nixon SJ, Zerial M, Parton RG
The Journal of biological chemistry280(51)42325-35(2005 Dec)

Rab18 localizes to lipid droplets and induces their close apposition to the endoplasmic reticulum-derived membrane.
Ozeki S, Cheng J, Tauchi-Sato K, Hatano N, Taniguchi H, Fujimoto T
Journal of cell science118(Pt 12)2601-11(2005 Jun)

Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes.
Brasaemle DL, Dolios G, Shapiro L, Wang R
The Journal of biological chemistry279(45)46835-42(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RAB18 regulates neuronal migration and morphogenesis during development.
Title: RAB18, a protein associated with Warburg Micro syndrome, controls neuronal migration in the developing cerebral cortex.

Retinoid and cholesterol metabolism are linked in stellate cells by the LD-associated protein Rab18 and may have significant therapeutic benefit in liver fibrosis.
Title: Liver X receptors balance lipid stores in hepatic stellate cells through Rab18, a retinoid responsive lipid droplet protein.

The results of this study indicated that the sensory nervous system is more vulnerable to Rab18 deficiency and WARBM is not only a neurodevelopmental but also neurodegenerative disease.
Title: ENU mutagenesis identifies mice modeling Warburg Micro Syndrome with sensory axon degeneration caused by a deletion in Rab18.

apparent similarities between the WARBM phenotype and the phenotype that we describe here indicate that the Rab18-mutant mouse provides an important platform for investigation of the disease pathogenesis and therapeutic interventions
Title: A novel mouse model of Warburg Micro syndrome reveals roles for RAB18 in eye development and organisation of the neuronal cytoskeleton.

Data suggest that overexpression of Rab18 in its GDP-bound state favors peroxisome proliferation in hepatocytes; Rab18 (as well as Rab6, Rab10, and Rab14) localizes to the peroxisomal compartment.
Title: Localization of Rab proteins to peroxisomes: a proteomics and immunofluorescence study.

The following Rab18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17662	NM_181070.6 Latest version!	Mus musculus RAB18, member RAS oncogene family (Rab18), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu17631	NM_001278447.1 Latest version!	Mus musculus RAB18, member RAS oncogene family (Rab18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab18 ( NM_181070.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_851415.1 Mus musculus RAB18, member RAS oncogene family (Rab18), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17662
Clone ID Related Accession (Same CDS sequence)	NM_181070.6
Accession Version	NM_181070.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-18 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC131796.2, BC056351.1 and AK034242.1. On Jun 7, 2013 this sequence version replaced NM_181070.5. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AW107089.1, SRR1660815.159668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGAGG ACGTGCTGAC CACTCTGAAG ATACTCATCA TCGGCGAGAG TGGGGTGGGC 
AAGTCCAGCC TGCTCCTGAG GTTCACAGAT GATACCTTTG ATCCAGAACT TGCAGCAACA 
ATAGGTGTTG ACTTTAAGGT GAAAACGATT TCAGTGGATG GAAATAAGGC TAAACTTGCA 
ATATGGGATA CAGCTGGTCA AGAGAGGTTC AGAACATTAA CTCCCAGCTA TTATAGAGGT 
GCACAGGGAG TTATATTAGT CTATGATGTC ACAAGAAGAG ACACCTTTGT TAAACTGGAT 
AACTGGTTAA ATGAATTGGA AACATACTGT ACAAGAAATG ACATAGTAAA CATGCTAGTT 
GGAAATAAAA TTGATAAGGA AAACCGTGAA GTCGATAGAA ATGAAGGCTT GAAATTTGCA 
CGCAAGCATT CTATGTTGTT CATAGAGGCA AGTGCAAAAA CCTGTGATGG TGTACAGTGT 
GCCTTTGAGG AGCTTGTTGA GAAGATCATT CAGACACCTG GCCTGTGGGA AAGTGAGAAC 
CAGAACAAAG GAGTCAAGCT GTCACACAGG GAAGAGAGCC GCGGAGGAGG CGCCTGCGGC 
GGTTACTGCT CTGTGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851415.1
CDS	99..719
Translation	MDEDVLTTLKILIIGESGVGKSSLLLRFTDDTFDPELAATIGVDFKVKTISVDGNKAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTRRDTFVKLDNWLNELETYCTRNDIVNMLVGNKIDKENREVDRNEGLKFARKHSMLFIEASAKTCDGVQCAFEELVEKIIQTPGLWESENQNKGVKLSHREESRGGGACGGYCSVL

Target ORF information:

RefSeq Version	NM_181070.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB18, member RAS oncogene family (Rab18), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181070.6

ORF Insert Sequence:

ATGGACGAGG ACGTGCTGAC CACTCTGAAG ATACTCATCA TCGGCGAGAG TGGGGTGGGC 
AAGTCCAGCC TGCTCCTGAG GTTCACAGAT GATACCTTTG ATCCAGAACT TGCAGCAACA 
ATAGGTGTTG ACTTTAAGGT GAAAACGATT TCAGTGGATG GAAATAAGGC TAAACTTGCA 
ATATGGGATA CAGCTGGTCA AGAGAGGTTC AGAACATTAA CTCCCAGCTA TTATAGAGGT 
GCACAGGGAG TTATATTAGT CTATGATGTC ACAAGAAGAG ACACCTTTGT TAAACTGGAT 
AACTGGTTAA ATGAATTGGA AACATACTGT ACAAGAAATG ACATAGTAAA CATGCTAGTT 
GGAAATAAAA TTGATAAGGA AAACCGTGAA GTCGATAGAA ATGAAGGCTT GAAATTTGCA 
CGCAAGCATT CTATGTTGTT CATAGAGGCA AGTGCAAAAA CCTGTGATGG TGTACAGTGT 
GCCTTTGAGG AGCTTGTTGA GAAGATCATT CAGACACCTG GCCTGTGGGA AAGTGAGAAC 
CAGAACAAAG GAGTCAAGCT GTCACACAGG GAAGAGAGCC GCGGAGGAGG CGCCTGCGGC 
GGTTACTGCT CTGTGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab18 ( NM_001278447.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001265376.1 Mus musculus RAB18, member RAS oncogene family (Rab18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17631
Clone ID Related Accession (Same CDS sequence)	NM_001278447.1
Accession Version	NM_001278447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-18 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC131796.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI646977.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGAGG ACGTGCTGAC CACTCTGAAG ATACTCATCA TCGGCGAGAG TGGGGTGGGC 
AAGTCCAGCC TGCTCCTGAG GTTCACAGAT GATACCTTTG ATCCAGAACT TGCAGCAACA 
ATAGAATTTT CATTTCTTCA AGGTGTTGAC TTTAAGGTGA AAACGATTTC AGTGGATGGA 
AATAAGGCTA AACTTGCAAT ATGGGATACA GCTGGTCAAG AGAGGTTCAG AACATTAACT 
CCCAGCTATT ATAGAGGTGC ACAGGGAGTT ATATTAGTCT ATGATGTCAC AAGAAGAGAC 
ACCTTTGTTA AACTGGATAA CTGGTTAAAT GAATTGGAAA CATACTGTAC AAGAAATGAC 
ATAGTAAACA TGCTAGTTGG AAATAAAATT GATAAGGAAA ACCGTGAAGT CGATAGAAAT 
GAAGGCTTGA AATTTGCACG CAAGCATTCT ATGTTGTTCA TAGAGGCAAG TGCAAAAACC 
TGTGATGGTG TACAGTGTGC CTTTGAGGAG CTTGTTGAGA AGATCATTCA GACACCTGGC 
CTGTGGGAAA GTGAGAACCA GAACAAAGGA GTCAAGCTGT CACACAGGGA AGAGAGCCGC 
GGAGGAGGCG CCTGCGGCGG TTACTGCTCT GTGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265376.1
CDS	99..737
Translation	MDEDVLTTLKILIIGESGVGKSSLLLRFTDDTFDPELAATIEFSFLQGVDFKVKTISVDGNKAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTRRDTFVKLDNWLNELETYCTRNDIVNMLVGNKIDKENREVDRNEGLKFARKHSMLFIEASAKTCDGVQCAFEELVEKIIQTPGLWESENQNKGVKLSHREESRGGGACGGYCSVL

Target ORF information:

RefSeq Version	NM_001278447.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB18, member RAS oncogene family (Rab18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278447.1

ORF Insert Sequence:

ATGGACGAGG ACGTGCTGAC CACTCTGAAG ATACTCATCA TCGGCGAGAG TGGGGTGGGC 
AAGTCCAGCC TGCTCCTGAG GTTCACAGAT GATACCTTTG ATCCAGAACT TGCAGCAACA 
ATAGAATTTT CATTTCTTCA AGGTGTTGAC TTTAAGGTGA AAACGATTTC AGTGGATGGA 
AATAAGGCTA AACTTGCAAT ATGGGATACA GCTGGTCAAG AGAGGTTCAG AACATTAACT 
CCCAGCTATT ATAGAGGTGC ACAGGGAGTT ATATTAGTCT ATGATGTCAC AAGAAGAGAC 
ACCTTTGTTA AACTGGATAA CTGGTTAAAT GAATTGGAAA CATACTGTAC AAGAAATGAC 
ATAGTAAACA TGCTAGTTGG AAATAAAATT GATAAGGAAA ACCGTGAAGT CGATAGAAAT 
GAAGGCTTGA AATTTGCACG CAAGCATTCT ATGTTGTTCA TAGAGGCAAG TGCAAAAACC 
TGTGATGGTG TACAGTGTGC CTTTGAGGAG CTTGTTGAGA AGATCATTCA GACACCTGGC 
CTGTGGGAAA GTGAGAACCA GAACAAAGGA GTCAAGCTGT CACACAGGGA AGAGAGCCGC 
GGAGGAGGCG CCTGCGGCGG TTACTGCTCT GTGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RAB18, a protein associated with Warburg Micro syndrome, controls neuronal migration in the developing cerebral cortex.
Molecular brain919(2016 Feb)
Wu Q,Sun X,Yue W,Lu T,Ruan Y,Chen T,Zhang D

Regulated localization of Rab18 to lipid droplets: effects of lipolytic stimulation and inhibition of lipid droplet catabolism.
The Journal of biological chemistry280(51)42325-35(2005 Dec)
Martin S,Driessen K,Nixon SJ,Zerial M,Parton RG

Rab18 localizes to lipid droplets and induces their close apposition to the endoplasmic reticulum-derived membrane.
Journal of cell science118(Pt 12)2601-11(2005 Jun)
Ozeki S,Cheng J,Tauchi-Sato K,Hatano N,Taniguchi H,Fujimoto T

Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes.
The Journal of biological chemistry279(45)46835-42(2004 Nov)
Brasaemle DL,Dolios G,Shapiro L,Wang R