Atp12a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp12a
Entrez Gene ID	192113
Full Name	ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Synonyms	Atp1al1,HKalpha2,cHKA
General protein information	Preferred Names ATPase, H+/K+ transporting, nongastric, alpha polypeptide Names potassium-transporting ATPase alpha chain 2 ATPase H+K+-transporting, alpha 2 non-gastric H(+)/K(+) ATPase subunit alpha proton pump
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030247692.1	XP_030103552.1	potassium-transporting ATPase alpha chain 2 isoform X1
XM_030247693.1	XP_030103553.1	potassium-transporting ATPase alpha chain 2 isoform X1
NM_138652.2	NP_619593.2	potassium-transporting ATPase alpha chain 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ATP12A	NP_001026080.1
Xenopus tropicalis (tropical clawed frog)	atp12a	NP_001025550.1
Canis lupus familiaris (dog)	ATP12A	XP_848278.2
Mus musculus (house mouse)	Atp12a	NP_619593.2
Bos taurus (cattle)	ATP12A	XP_002691916.2
Rattus norvegicus (Norway rat)	Atp12a	NP_598201.1
Homo sapiens (human)	ATP12A	NP_001172014.1
Pan troglodytes (chimpanzee)	ATP12A	XP_003314125.1
	LOC721488	XP_001117656.1

Gene Ontology
Bibliography

Related articles in PubMed

H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction.
Walter C, Tanfous MB, Igoudjil K, Salhi A, Escher G, Crambert G
Pflugers Archiv : European journal of physiology468(10)1673-83(2016 Oct)

A link between fertility and K+ homeostasis: role of the renal H,K-ATPase type 2.
Salhi A, Lamouroux C, Pestov NB, Modyanov NN, Doucet A, Crambert G
Pflugers Archiv : European journal of physiology465(8)1149-58(2013 Aug)

Circadian expression of H,K-ATPase type 2 contributes to the stability of plasma K? levels.
Salhi A, Centeno G, Firsov D, Crambert G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(7)2859-67(2012 Jul)

Mineralocorticoids stimulate the activity and expression of renal H+,K+-ATPases.
Greenlee MM, Lynch IJ, Gumz ML, Cain BD, Wingo CS
Journal of the American Society of Nephrology : JASN22(1)49-58(2011 Jan)

Sp1 trans-activates the murine H(+)-K(+)-ATPase alpha(2)-subunit gene.
Yu Z, Li M, Zhang D, Xu W, Kone BC
American journal of physiology. Renal physiology297(1)F63-70(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

After 4 days of K+ restriction HKA2 KO mice exhibit hypokalemia and hypotension.
Title: H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction.

HKA2-null mice did not accumulate K(+) in their extracellular compartment.
Title: A link between fertility and K+ homeostasis: role of the renal H,K-ATPase type 2.

HKA2-null mice fed a normal diet have 2-fold higher K(+) renal excretion during rest. Under K(+) restriction, their urinary K(+) loss is 40% higher during the activity period.
Title: Circadian expression of H,K-ATPase type 2 contributes to the stability of plasma K? levels.

Sp1 plays an important and positive role in controlling basal HKalpha2 gene expression in murine inner medullary collecting duct cells in vivo and in vitro.
Title: Sp1 trans-activates the murine H(+)-K(+)-ATPase alpha(2)-subunit gene.

Both HKalpha(1) and HKalpha(2) contribute to proton secretion in both A-type and B-type intercalated cells in animals fed a normal diet.
Title: Impaired acid secretion in cortical collecting duct intercalated cells from H-K-ATPase-deficient mice: role of HKalpha isoforms.

The following Atp12a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp12a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19509	NM_138652.2 Latest version!	Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$608.30 $869.00
OMu19509	XM_030247693.1 Latest version!	Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu19509	XM_030247692.1 Latest version!	Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp12a ( NM_138652.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_619593.2 Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), mRNA.

Price & Availability↑

CloneID	OMu19509
Clone ID Related Accession (Same CDS sequence)	XM_030247692.1 , XM_030247693.1 , NM_138652.2
Accession Version	NM_138652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	potassium-transporting ATPase alpha chain 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC109011.1 and BY663985.1. On Sep 13, 2007 this sequence version replaced NM_138652.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109011.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGCGCCGGA AAACAGAAAT CTATTCTGTA GAGCTCAATG GAACTAAAGA CGTCGAGCTG 
GCAGACCAGA AAGATGACAA AAAGTTCAAG GGCGGGAAGA ACAAAGACTC AGAACCCAAC 
AAGAGTCAGG AGGAGGAGCT GAAGAAAGAA CTTGATCTGG ATGACCACAG ACTCAGTAAT 
ACAGATTTGG AACAGAAATA TGGTACAAAC ATCATCCAGG GTCTCTCCAG TATCAGAGCT 
GCAGAGCTCC TGGCTCGGGA TGGGCCCAAC GCCCTCACCC CTCCCAAGCA AACTCCAGAG 
ATCATCAAGT TCCTCAAACA GATGGTGGGG GGCTTTTCCA TCCTTCTGTG GATAGGTGCA 
GCCTTGTGCT GGATCGCATA TGTGATCCAG TATGTCAGCA GTACTGCATC CCTGGACAAT 
GTCTATTTGG GCGCCATACT TGTCCTGGTC GTTATTTTAA CGGGGATTTT TGCTTATTAC 
CAAGAAGCCA AAAGTACCAA CATTATGGCA AGCTTTAGTA AGATGATCCC CCAGCAAGCT 
CTTGTCATCC GAGATGCAGA AAAGAAGATC ATTCCAGCAG AGCAGCTGGT GGTGGGGGAC 
GTTGTGGAGA TTAAAGGAGG GGACCAGATC CCTGCAGACA TCAGACTGGT GTTTTCCCAA 
GGGTGCAAAG TAGATAACTC CTCTCTCACT GGGGAGTCTG AGCCCCAGGC TCGGTCAACT 
GAGTTTACCC ATGAAAACCC CTTGGAAACT AAGAACATAG GCTTCTATTC TACAACGTGC 
CTGGAAGGTA CGGCAACTGG CATTGTCATC AACACGGGTG ACCGCACCAT CATCGGGCGC 
ATAGCCTCCT TGGCTTCAGG CGTTGGGAGT GAGAAGACAC CCATAGCCAT TGAGATTGAG 
CATTTTGTCC ATATTGTGGC AGCAGTGGCT GTGTCCGTTG GCGTCATTTT CTTCATCACC 
GCCGTGTGCA TGAAGTACTA CGTCCTGGAT GCCATCATCT TCCTCATTAG CATCATTGTG 
GCCAATGTGC CAGAGGGCCT CCTGGCTACT GTCACTGTTA CCCTGTCACT GACGGCAAAG 
CGGATGGCTA AGAAGAACTG CTTGGTAAAG AACCTGGAGG CCGTGGAGAC GCTTGGCTCC 
ACCTCCATCA TCTGCTCAGA TAAGACAGGG ACCCTCACCC AGAACAGGAT GACTGTGGCC 
CACTTGTGGT TTGACAACCA GATCTTTGTG GCTGATACAA GTGAAAACCA GACAAAACAA 
GCCTTTGACC AAAGCTCTGG AACCTGGGCT TCCTTGTCCA AGATAATAAC ACTGTGTAAC 
CGAGCAGAGT TCAGGCCAGG CCAGGAGAGT GTCCCCATCA TGAAGAGAGT TGTGGTTGGA 
GATGCCTCAG AAACTGCTCT GCTAAAGTTC TCAGAGGTTA TTTTGGGTGA TGTGATGGAC 
ATTAGGAAAA GAAACCACAA AGTAGCTGAG ATTCCTTTTA ATTCTACCAA CAAATTTCAG 
CTCTCCATAC ATGAGACCGA GGACCCGAAT GACAAGCGCT TCCTCATGGT GATGAAAGGG 
GCCCCTGAGA GGATCTTAGA GAAGTGCAGC ACCATCATGA TCAACGGCCA GGAGCAGCCA 
CTGGACAAGA GCTCTGCCGA TGCCTTCCAC ACGGCCTACA TGGAGCTGGG TGGTCTGGGA 
GAGCGTGTGT TGGGTTTCTG TCACCTCTAC TTGCCAGCAG ATAAGTTTCC CCAAAGCTAC 
ACATTTGATG TAGACTCCAT AAACTTTCCC ACTTCCAATC TCTGCTTTGT GGGGCTTCTG 
TCCATGATTG ACCCTCCTCG GTCTACCGTG CCAGATGCGG TCTCCAAATG TCGGAGTGCG 
GGGATCAAGG TTATCATGGT CACAGGTGAT CACCCTATCA CAGCCAAAGC TATTGCCAAG 
AGTGTAGGAA TCATATCAGC CAACAATGAG ACGGTGGAAG ACATCGCAAA ACGCCGCAAC 
ATTGCTGTGG AGCAAGTCAA CAAAAGAGAA GCGAAAGCCG CTGTGGTGAC TGGCATGGAA 
CTGAAGGACA TGACCCCAGA ACAACTGGAT GAGCTTCTAA TCAACTACCA AGAAATTGTG 
TTTGCCCGGA CATCACCCCA GCAGAAGTTG ATCATTGTAG AAGGCTGTCA GAGGCAGGAT 
GCAGTTGTTG CTGTGACGGG TGATGGAGTA AACGACTCTC CAGCATTAAA GAAGGCAGAC 
ATCGGGATTG CTATGGGGAT AGCAGGTTCT GATGCTGCTA AGAATGCAGC TGACATGGTC 
TTGCTCGATG ACAACTTTGC ATCTATAGTC ACAGGGGTGG AGGAAGGTCG CCTGATCTTC 
GACAACCTGA AGAAGACCAT TGCTTACACC CTGACAAAGA ACATCGCTGA GCTCTGCCCT 
TTTTTGATCT ACATTGTTGC TGGGCTCCCC CTGCCTATTG GCACCATCAC TATCCTATTC 
ATCGACCTGG GCACAGACAT TATCCCCTCC ATTGCCTTGG CCTATGAGAA AGCGGAAAGT 
GACATTATGA ATAGGAAACC TCGCCACAAG AAGAAAGACA GACTGGTGAA CAAGCAGCTG 
GCCATCTACT CTTACCTTCA CATTGGTCTC ATGCAAGCCC TGGGAGGTTT CCTCGTCTAT 
TTCACTGTCT ATGCACAGCA GGGCTTTTGG CCCACCTCTC TCATCAACCT GAGGGTATCA 
TGGGAAACAG ATGACATAAA TGACTTGGAA GATAGCTACG GGCAGGAATG GACGAGGTAT 
CAGAGAAAAT ACCTGGAATG GACAGGCTCA ACGGCTTTCT TTGTTGCCAT CATGGTCCAA 
CAAATTGCAG ATCTGATCAT CAGGAAAACC CGGAGGAATT CCATCTTCCA GCAGGGGCTC 
TTCAGAAACA AAGTCATCTG GGTGGGGATC ATCTCGCAGA TCATCGTTGC GTTGGTCCTC 
TCCTACGGTC TCGGGAGTGT CACAGCTCTG AGCTTTACCA TGCTCAGGGC TCAGTACTGG 
TTTGTGGCTG TCCCGCATGC CATTTTGATT TGGGTGTATG ACGAGATGCG GAAGTTGTTC 
ATCAGGCTCT ACCCTGGAAG CTGGTGGGAC AAGAACATGT ATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619593.2
CDS	214..3321
Translation	MRRKTEIYSVELNGTKDVELADQKDDKKFKGGKNKDSEPNKSQEEELKKELDLDDHRLSNTDLEQKYGTNIIQGLSSIRAAELLARDGPNALTPPKQTPEIIKFLKQMVGGFSILLWIGAALCWIAYVIQYVSSTASLDNVYLGAILVLVVILTGIFAYYQEAKSTNIMASFSKMIPQQALVIRDAEKKIIPAEQLVVGDVVEIKGGDQIPADIRLVFSQGCKVDNSSLTGESEPQARSTEFTHENPLETKNIGFYSTTCLEGTATGIVINTGDRTIIGRIASLASGVGSEKTPIAIEIEHFVHIVAAVAVSVGVIFFITAVCMKYYVLDAIIFLISIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQESVPIMKRVVVGDASETALLKFSEVILGDVMDIRKRNHKVAEIPFNSTNKFQLSIHETEDPNDKRFLMVMKGAPERILEKCSTIMINGQEQPLDKSSADAFHTAYMELGGLGERVLGFCHLYLPADKFPQSYTFDVDSINFPTSNLCFVGLLSMIDPPRSTVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVNKREAKAAVVTGMELKDMTPEQLDELLINYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAELCPFLIYIVAGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKDRLVNKQLAIYSYLHIGLMQALGGFLVYFTVYAQQGFWPTSLINLRVSWETDDINDLEDSYGQEWTRYQRKYLEWTGSTAFFVAIMVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGIISQIIVALVLSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEMRKLFIRLYPGSWWDKNMYY

Target ORF information:

RefSeq Version	NM_138652.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138652.2

ORF Insert Sequence:

ATGCGCCGGA AAACAGAAAT CTATTCTGTA GAGCTCAATG GAACTAAAGA CGTCGAGCTG 
GCAGACCAGA AAGATGACAA AAAGTTCAAG GGCGGGAAGA ACAAAGACTC AGAACCCAAC 
AAGAGTCAGG AGGAGGAGCT GAAGAAAGAA CTTGATCTGG ATGACCACAG ACTCAGTAAT 
ACAGATTTGG AACAGAAATA TGGTACAAAC ATCATCCAGG GTCTCTCCAG TATCAGAGCT 
GCAGAGCTCC TGGCTCGGGA TGGGCCCAAC GCCCTCACCC CTCCCAAGCA AACTCCAGAG 
ATCATCAAGT TCCTCAAACA GATGGTGGGG GGCTTTTCCA TCCTTCTGTG GATAGGTGCA 
GCCTTGTGCT GGATCGCATA TGTGATCCAG TATGTCAGCA GTACTGCATC CCTGGACAAT 
GTCTATTTGG GCGCCATACT TGTCCTGGTC GTTATTTTAA CGGGGATTTT TGCTTATTAC 
CAAGAAGCCA AAAGTACCAA CATTATGGCA AGCTTTAGTA AGATGATCCC CCAGCAAGCT 
CTTGTCATCC GAGATGCAGA AAAGAAGATC ATTCCAGCAG AGCAGCTGGT GGTGGGGGAC 
GTTGTGGAGA TTAAAGGAGG GGACCAGATC CCTGCAGACA TCAGACTGGT GTTTTCCCAA 
GGGTGCAAAG TAGATAACTC CTCTCTCACT GGGGAGTCTG AGCCCCAGGC TCGGTCAACT 
GAGTTTACCC ATGAAAACCC CTTGGAAACT AAGAACATAG GCTTCTATTC TACAACGTGC 
CTGGAAGGTA CGGCAACTGG CATTGTCATC AACACGGGTG ACCGCACCAT CATCGGGCGC 
ATAGCCTCCT TGGCTTCAGG CGTTGGGAGT GAGAAGACAC CCATAGCCAT TGAGATTGAG 
CATTTTGTCC ATATTGTGGC AGCAGTGGCT GTGTCCGTTG GCGTCATTTT CTTCATCACC 
GCCGTGTGCA TGAAGTACTA CGTCCTGGAT GCCATCATCT TCCTCATTAG CATCATTGTG 
GCCAATGTGC CAGAGGGCCT CCTGGCTACT GTCACTGTTA CCCTGTCACT GACGGCAAAG 
CGGATGGCTA AGAAGAACTG CTTGGTAAAG AACCTGGAGG CCGTGGAGAC GCTTGGCTCC 
ACCTCCATCA TCTGCTCAGA TAAGACAGGG ACCCTCACCC AGAACAGGAT GACTGTGGCC 
CACTTGTGGT TTGACAACCA GATCTTTGTG GCTGATACAA GTGAAAACCA GACAAAACAA 
GCCTTTGACC AAAGCTCTGG AACCTGGGCT TCCTTGTCCA AGATAATAAC ACTGTGTAAC 
CGAGCAGAGT TCAGGCCAGG CCAGGAGAGT GTCCCCATCA TGAAGAGAGT TGTGGTTGGA 
GATGCCTCAG AAACTGCTCT GCTAAAGTTC TCAGAGGTTA TTTTGGGTGA TGTGATGGAC 
ATTAGGAAAA GAAACCACAA AGTAGCTGAG ATTCCTTTTA ATTCTACCAA CAAATTTCAG 
CTCTCCATAC ATGAGACCGA GGACCCGAAT GACAAGCGCT TCCTCATGGT GATGAAAGGG 
GCCCCTGAGA GGATCTTAGA GAAGTGCAGC ACCATCATGA TCAACGGCCA GGAGCAGCCA 
CTGGACAAGA GCTCTGCCGA TGCCTTCCAC ACGGCCTACA TGGAGCTGGG TGGTCTGGGA 
GAGCGTGTGT TGGGTTTCTG TCACCTCTAC TTGCCAGCAG ATAAGTTTCC CCAAAGCTAC 
ACATTTGATG TAGACTCCAT AAACTTTCCC ACTTCCAATC TCTGCTTTGT GGGGCTTCTG 
TCCATGATTG ACCCTCCTCG GTCTACCGTG CCAGATGCGG TCTCCAAATG TCGGAGTGCG 
GGGATCAAGG TTATCATGGT CACAGGTGAT CACCCTATCA CAGCCAAAGC TATTGCCAAG 
AGTGTAGGAA TCATATCAGC CAACAATGAG ACGGTGGAAG ACATCGCAAA ACGCCGCAAC 
ATTGCTGTGG AGCAAGTCAA CAAAAGAGAA GCGAAAGCCG CTGTGGTGAC TGGCATGGAA 
CTGAAGGACA TGACCCCAGA ACAACTGGAT GAGCTTCTAA TCAACTACCA AGAAATTGTG 
TTTGCCCGGA CATCACCCCA GCAGAAGTTG ATCATTGTAG AAGGCTGTCA GAGGCAGGAT 
GCAGTTGTTG CTGTGACGGG TGATGGAGTA AACGACTCTC CAGCATTAAA GAAGGCAGAC 
ATCGGGATTG CTATGGGGAT AGCAGGTTCT GATGCTGCTA AGAATGCAGC TGACATGGTC 
TTGCTCGATG ACAACTTTGC ATCTATAGTC ACAGGGGTGG AGGAAGGTCG CCTGATCTTC 
GACAACCTGA AGAAGACCAT TGCTTACACC CTGACAAAGA ACATCGCTGA GCTCTGCCCT 
TTTTTGATCT ACATTGTTGC TGGGCTCCCC CTGCCTATTG GCACCATCAC TATCCTATTC 
ATCGACCTGG GCACAGACAT TATCCCCTCC ATTGCCTTGG CCTATGAGAA AGCGGAAAGT 
GACATTATGA ATAGGAAACC TCGCCACAAG AAGAAAGACA GACTGGTGAA CAAGCAGCTG 
GCCATCTACT CTTACCTTCA CATTGGTCTC ATGCAAGCCC TGGGAGGTTT CCTCGTCTAT 
TTCACTGTCT ATGCACAGCA GGGCTTTTGG CCCACCTCTC TCATCAACCT GAGGGTATCA 
TGGGAAACAG ATGACATAAA TGACTTGGAA GATAGCTACG GGCAGGAATG GACGAGGTAT 
CAGAGAAAAT ACCTGGAATG GACAGGCTCA ACGGCTTTCT TTGTTGCCAT CATGGTCCAA 
CAAATTGCAG ATCTGATCAT CAGGAAAACC CGGAGGAATT CCATCTTCCA GCAGGGGCTC 
TTCAGAAACA AAGTCATCTG GGTGGGGATC ATCTCGCAGA TCATCGTTGC GTTGGTCCTC 
TCCTACGGTC TCGGGAGTGT CACAGCTCTG AGCTTTACCA TGCTCAGGGC TCAGTACTGG 
TTTGTGGCTG TCCCGCATGC CATTTTGATT TGGGTGTATG ACGAGATGCG GAAGTTGTTC 
ATCAGGCTCT ACCCTGGAAG CTGGTGGGAC AAGAACATGT ATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp12a ( XM_030247692.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103552.1 Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19509
Clone ID Related Accession (Same CDS sequence)	XM_030247692.1 , XM_030247693.1 , NM_138652.2
Accession Version	XM_030247692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium-transporting ATPase alpha chain 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGGA AAACAGAAAT CTATTCTGTA GAGCTCAATG GAACTAAAGA CGTCGAGCTG 
GCAGACCAGA AAGATGACAA AAAGTTCAAG GGCGGGAAGA ACAAAGACTC AGAACCCAAC 
AAGAGTCAGG AGGAGGAGCT GAAGAAAGAA CTTGATCTGG ATGACCACAG ACTCAGTAAT 
ACAGATTTGG AACAGAAATA TGGTACAAAC ATCATCCAGG GTCTCTCCAG TATCAGAGCT 
GCAGAGCTCC TGGCTCGGGA TGGGCCCAAC GCCCTCACCC CTCCCAAGCA AACTCCAGAG 
ATCATCAAGT TCCTCAAACA GATGGTGGGG GGCTTTTCCA TCCTTCTGTG GATAGGTGCA 
GCCTTGTGCT GGATCGCATA TGTGATCCAG TATGTCAGCA GTACTGCATC CCTGGACAAT 
GTCTATTTGG GCGCCATACT TGTCCTGGTC GTTATTTTAA CGGGGATTTT TGCTTATTAC 
CAAGAAGCCA AAAGTACCAA CATTATGGCA AGCTTTAGTA AGATGATCCC CCAGCAAGCT 
CTTGTCATCC GAGATGCAGA AAAGAAGATC ATTCCAGCAG AGCAGCTGGT GGTGGGGGAC 
GTTGTGGAGA TTAAAGGAGG GGACCAGATC CCTGCAGACA TCAGACTGGT GTTTTCCCAA 
GGGTGCAAAG TAGATAACTC CTCTCTCACT GGGGAGTCTG AGCCCCAGGC TCGGTCAACT 
GAGTTTACCC ATGAAAACCC CTTGGAAACT AAGAACATAG GCTTCTATTC TACAACGTGC 
CTGGAAGGTA CGGCAACTGG CATTGTCATC AACACGGGTG ACCGCACCAT CATCGGGCGC 
ATAGCCTCCT TGGCTTCAGG CGTTGGGAGT GAGAAGACAC CCATAGCCAT TGAGATTGAG 
CATTTTGTCC ATATTGTGGC AGCAGTGGCT GTGTCCGTTG GCGTCATTTT CTTCATCACC 
GCCGTGTGCA TGAAGTACTA CGTCCTGGAT GCCATCATCT TCCTCATTAG CATCATTGTG 
GCCAATGTGC CAGAGGGCCT CCTGGCTACT GTCACTGTTA CCCTGTCACT GACGGCAAAG 
CGGATGGCTA AGAAGAACTG CTTGGTAAAG AACCTGGAGG CCGTGGAGAC GCTTGGCTCC 
ACCTCCATCA TCTGCTCAGA TAAGACAGGG ACCCTCACCC AGAACAGGAT GACTGTGGCC 
CACTTGTGGT TTGACAACCA GATCTTTGTG GCTGATACAA GTGAAAACCA GACAAAACAA 
GCCTTTGACC AAAGCTCTGG AACCTGGGCT TCCTTGTCCA AGATAATAAC ACTGTGTAAC 
CGAGCAGAGT TCAGGCCAGG CCAGGAGAGT GTCCCCATCA TGAAGAGAGT TGTGGTTGGA 
GATGCCTCAG AAACTGCTCT GCTAAAGTTC TCAGAGGTTA TTTTGGGTGA TGTGATGGAC 
ATTAGGAAAA GAAACCACAA AGTAGCTGAG ATTCCTTTTA ATTCTACCAA CAAATTTCAG 
CTCTCCATAC ATGAGACCGA GGACCCGAAT GACAAGCGCT TCCTCATGGT GATGAAAGGG 
GCCCCTGAGA GGATCTTAGA GAAGTGCAGC ACCATCATGA TCAACGGCCA GGAGCAGCCA 
CTGGACAAGA GCTCTGCCGA TGCCTTCCAC ACGGCCTACA TGGAGCTGGG TGGTCTGGGA 
GAGCGTGTGT TGGGTTTCTG TCACCTCTAC TTGCCAGCAG ATAAGTTTCC CCAAAGCTAC 
ACATTTGATG TAGACTCCAT AAACTTTCCC ACTTCCAATC TCTGCTTTGT GGGGCTTCTG 
TCCATGATTG ACCCTCCTCG GTCTACCGTG CCAGATGCGG TCTCCAAATG TCGGAGTGCG 
GGGATCAAGG TTATCATGGT CACAGGTGAT CACCCTATCA CAGCCAAAGC TATTGCCAAG 
AGTGTAGGAA TCATATCAGC CAACAATGAG ACGGTGGAAG ACATCGCAAA ACGCCGCAAC 
ATTGCTGTGG AGCAAGTCAA CAAAAGAGAA GCGAAAGCCG CTGTGGTGAC TGGCATGGAA 
CTGAAGGACA TGACCCCAGA ACAACTGGAT GAGCTTCTAA TCAACTACCA AGAAATTGTG 
TTTGCCCGGA CATCACCCCA GCAGAAGTTG ATCATTGTAG AAGGCTGTCA GAGGCAGGAT 
GCAGTTGTTG CTGTGACGGG TGATGGAGTA AACGACTCTC CAGCATTAAA GAAGGCAGAC 
ATCGGGATTG CTATGGGGAT AGCAGGTTCT GATGCTGCTA AGAATGCAGC TGACATGGTC 
TTGCTCGATG ACAACTTTGC ATCTATAGTC ACAGGGGTGG AGGAAGGTCG CCTGATCTTC 
GACAACCTGA AGAAGACCAT TGCTTACACC CTGACAAAGA ACATCGCTGA GCTCTGCCCT 
TTTTTGATCT ACATTGTTGC TGGGCTCCCC CTGCCTATTG GCACCATCAC TATCCTATTC 
ATCGACCTGG GCACAGACAT TATCCCCTCC ATTGCCTTGG CCTATGAGAA AGCGGAAAGT 
GACATTATGA ATAGGAAACC TCGCCACAAG AAGAAAGACA GACTGGTGAA CAAGCAGCTG 
GCCATCTACT CTTACCTTCA CATTGGTCTC ATGCAAGCCC TGGGAGGTTT CCTCGTCTAT 
TTCACTGTCT ATGCACAGCA GGGCTTTTGG CCCACCTCTC TCATCAACCT GAGGGTATCA 
TGGGAAACAG ATGACATAAA TGACTTGGAA GATAGCTACG GGCAGGAATG GACGAGGTAT 
CAGAGAAAAT ACCTGGAATG GACAGGCTCA ACGGCTTTCT TTGTTGCCAT CATGGTCCAA 
CAAATTGCAG ATCTGATCAT CAGGAAAACC CGGAGGAATT CCATCTTCCA GCAGGGGCTC 
TTCAGAAACA AAGTCATCTG GGTGGGGATC ATCTCGCAGA TCATCGTTGC GTTGGTCCTC 
TCCTACGGTC TCGGGAGTGT CACAGCTCTG AGCTTTACCA TGCTCAGGGC TCAGTACTGG 
TTTGTGGCTG TCCCGCATGC CATTTTGATT TGGGTGTATG ACGAGATGCG GAAGTTGTTC 
ATCAGGCTCT ACCCTGGAAG CTGGTGGGAC AAGAACATGT ATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103552.1
CDS	293..3400
Translation	MRRKTEIYSVELNGTKDVELADQKDDKKFKGGKNKDSEPNKSQEEELKKELDLDDHRLSNTDLEQKYGTNIIQGLSSIRAAELLARDGPNALTPPKQTPEIIKFLKQMVGGFSILLWIGAALCWIAYVIQYVSSTASLDNVYLGAILVLVVILTGIFAYYQEAKSTNIMASFSKMIPQQALVIRDAEKKIIPAEQLVVGDVVEIKGGDQIPADIRLVFSQGCKVDNSSLTGESEPQARSTEFTHENPLETKNIGFYSTTCLEGTATGIVINTGDRTIIGRIASLASGVGSEKTPIAIEIEHFVHIVAAVAVSVGVIFFITAVCMKYYVLDAIIFLISIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQESVPIMKRVVVGDASETALLKFSEVILGDVMDIRKRNHKVAEIPFNSTNKFQLSIHETEDPNDKRFLMVMKGAPERILEKCSTIMINGQEQPLDKSSADAFHTAYMELGGLGERVLGFCHLYLPADKFPQSYTFDVDSINFPTSNLCFVGLLSMIDPPRSTVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVNKREAKAAVVTGMELKDMTPEQLDELLINYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAELCPFLIYIVAGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKDRLVNKQLAIYSYLHIGLMQALGGFLVYFTVYAQQGFWPTSLINLRVSWETDDINDLEDSYGQEWTRYQRKYLEWTGSTAFFVAIMVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGIISQIIVALVLSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEMRKLFIRLYPGSWWDKNMYY

Target ORF information:

RefSeq Version	XM_030247692.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247692.1

ORF Insert Sequence:

ATGCGCCGGA AAACAGAAAT CTATTCTGTA GAGCTCAATG GAACTAAAGA CGTCGAGCTG 
GCAGACCAGA AAGATGACAA AAAGTTCAAG GGCGGGAAGA ACAAAGACTC AGAACCCAAC 
AAGAGTCAGG AGGAGGAGCT GAAGAAAGAA CTTGATCTGG ATGACCACAG ACTCAGTAAT 
ACAGATTTGG AACAGAAATA TGGTACAAAC ATCATCCAGG GTCTCTCCAG TATCAGAGCT 
GCAGAGCTCC TGGCTCGGGA TGGGCCCAAC GCCCTCACCC CTCCCAAGCA AACTCCAGAG 
ATCATCAAGT TCCTCAAACA GATGGTGGGG GGCTTTTCCA TCCTTCTGTG GATAGGTGCA 
GCCTTGTGCT GGATCGCATA TGTGATCCAG TATGTCAGCA GTACTGCATC CCTGGACAAT 
GTCTATTTGG GCGCCATACT TGTCCTGGTC GTTATTTTAA CGGGGATTTT TGCTTATTAC 
CAAGAAGCCA AAAGTACCAA CATTATGGCA AGCTTTAGTA AGATGATCCC CCAGCAAGCT 
CTTGTCATCC GAGATGCAGA AAAGAAGATC ATTCCAGCAG AGCAGCTGGT GGTGGGGGAC 
GTTGTGGAGA TTAAAGGAGG GGACCAGATC CCTGCAGACA TCAGACTGGT GTTTTCCCAA 
GGGTGCAAAG TAGATAACTC CTCTCTCACT GGGGAGTCTG AGCCCCAGGC TCGGTCAACT 
GAGTTTACCC ATGAAAACCC CTTGGAAACT AAGAACATAG GCTTCTATTC TACAACGTGC 
CTGGAAGGTA CGGCAACTGG CATTGTCATC AACACGGGTG ACCGCACCAT CATCGGGCGC 
ATAGCCTCCT TGGCTTCAGG CGTTGGGAGT GAGAAGACAC CCATAGCCAT TGAGATTGAG 
CATTTTGTCC ATATTGTGGC AGCAGTGGCT GTGTCCGTTG GCGTCATTTT CTTCATCACC 
GCCGTGTGCA TGAAGTACTA CGTCCTGGAT GCCATCATCT TCCTCATTAG CATCATTGTG 
GCCAATGTGC CAGAGGGCCT CCTGGCTACT GTCACTGTTA CCCTGTCACT GACGGCAAAG 
CGGATGGCTA AGAAGAACTG CTTGGTAAAG AACCTGGAGG CCGTGGAGAC GCTTGGCTCC 
ACCTCCATCA TCTGCTCAGA TAAGACAGGG ACCCTCACCC AGAACAGGAT GACTGTGGCC 
CACTTGTGGT TTGACAACCA GATCTTTGTG GCTGATACAA GTGAAAACCA GACAAAACAA 
GCCTTTGACC AAAGCTCTGG AACCTGGGCT TCCTTGTCCA AGATAATAAC ACTGTGTAAC 
CGAGCAGAGT TCAGGCCAGG CCAGGAGAGT GTCCCCATCA TGAAGAGAGT TGTGGTTGGA 
GATGCCTCAG AAACTGCTCT GCTAAAGTTC TCAGAGGTTA TTTTGGGTGA TGTGATGGAC 
ATTAGGAAAA GAAACCACAA AGTAGCTGAG ATTCCTTTTA ATTCTACCAA CAAATTTCAG 
CTCTCCATAC ATGAGACCGA GGACCCGAAT GACAAGCGCT TCCTCATGGT GATGAAAGGG 
GCCCCTGAGA GGATCTTAGA GAAGTGCAGC ACCATCATGA TCAACGGCCA GGAGCAGCCA 
CTGGACAAGA GCTCTGCCGA TGCCTTCCAC ACGGCCTACA TGGAGCTGGG TGGTCTGGGA 
GAGCGTGTGT TGGGTTTCTG TCACCTCTAC TTGCCAGCAG ATAAGTTTCC CCAAAGCTAC 
ACATTTGATG TAGACTCCAT AAACTTTCCC ACTTCCAATC TCTGCTTTGT GGGGCTTCTG 
TCCATGATTG ACCCTCCTCG GTCTACCGTG CCAGATGCGG TCTCCAAATG TCGGAGTGCG 
GGGATCAAGG TTATCATGGT CACAGGTGAT CACCCTATCA CAGCCAAAGC TATTGCCAAG 
AGTGTAGGAA TCATATCAGC CAACAATGAG ACGGTGGAAG ACATCGCAAA ACGCCGCAAC 
ATTGCTGTGG AGCAAGTCAA CAAAAGAGAA GCGAAAGCCG CTGTGGTGAC TGGCATGGAA 
CTGAAGGACA TGACCCCAGA ACAACTGGAT GAGCTTCTAA TCAACTACCA AGAAATTGTG 
TTTGCCCGGA CATCACCCCA GCAGAAGTTG ATCATTGTAG AAGGCTGTCA GAGGCAGGAT 
GCAGTTGTTG CTGTGACGGG TGATGGAGTA AACGACTCTC CAGCATTAAA GAAGGCAGAC 
ATCGGGATTG CTATGGGGAT AGCAGGTTCT GATGCTGCTA AGAATGCAGC TGACATGGTC 
TTGCTCGATG ACAACTTTGC ATCTATAGTC ACAGGGGTGG AGGAAGGTCG CCTGATCTTC 
GACAACCTGA AGAAGACCAT TGCTTACACC CTGACAAAGA ACATCGCTGA GCTCTGCCCT 
TTTTTGATCT ACATTGTTGC TGGGCTCCCC CTGCCTATTG GCACCATCAC TATCCTATTC 
ATCGACCTGG GCACAGACAT TATCCCCTCC ATTGCCTTGG CCTATGAGAA AGCGGAAAGT 
GACATTATGA ATAGGAAACC TCGCCACAAG AAGAAAGACA GACTGGTGAA CAAGCAGCTG 
GCCATCTACT CTTACCTTCA CATTGGTCTC ATGCAAGCCC TGGGAGGTTT CCTCGTCTAT 
TTCACTGTCT ATGCACAGCA GGGCTTTTGG CCCACCTCTC TCATCAACCT GAGGGTATCA 
TGGGAAACAG ATGACATAAA TGACTTGGAA GATAGCTACG GGCAGGAATG GACGAGGTAT 
CAGAGAAAAT ACCTGGAATG GACAGGCTCA ACGGCTTTCT TTGTTGCCAT CATGGTCCAA 
CAAATTGCAG ATCTGATCAT CAGGAAAACC CGGAGGAATT CCATCTTCCA GCAGGGGCTC 
TTCAGAAACA AAGTCATCTG GGTGGGGATC ATCTCGCAGA TCATCGTTGC GTTGGTCCTC 
TCCTACGGTC TCGGGAGTGT CACAGCTCTG AGCTTTACCA TGCTCAGGGC TCAGTACTGG 
TTTGTGGCTG TCCCGCATGC CATTTTGATT TGGGTGTATG ACGAGATGCG GAAGTTGTTC 
ATCAGGCTCT ACCCTGGAAG CTGGTGGGAC AAGAACATGT ATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp12a ( XM_030247693.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103553.1 Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant X2, mRNA.

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CloneID	OMu19509
Clone ID Related Accession (Same CDS sequence)	XM_030247692.1 , XM_030247693.1 , NM_138652.2
Accession Version	XM_030247693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium-transporting ATPase alpha chain 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGGA AAACAGAAAT CTATTCTGTA GAGCTCAATG GAACTAAAGA CGTCGAGCTG 
GCAGACCAGA AAGATGACAA AAAGTTCAAG GGCGGGAAGA ACAAAGACTC AGAACCCAAC 
AAGAGTCAGG AGGAGGAGCT GAAGAAAGAA CTTGATCTGG ATGACCACAG ACTCAGTAAT 
ACAGATTTGG AACAGAAATA TGGTACAAAC ATCATCCAGG GTCTCTCCAG TATCAGAGCT 
GCAGAGCTCC TGGCTCGGGA TGGGCCCAAC GCCCTCACCC CTCCCAAGCA AACTCCAGAG 
ATCATCAAGT TCCTCAAACA GATGGTGGGG GGCTTTTCCA TCCTTCTGTG GATAGGTGCA 
GCCTTGTGCT GGATCGCATA TGTGATCCAG TATGTCAGCA GTACTGCATC CCTGGACAAT 
GTCTATTTGG GCGCCATACT TGTCCTGGTC GTTATTTTAA CGGGGATTTT TGCTTATTAC 
CAAGAAGCCA AAAGTACCAA CATTATGGCA AGCTTTAGTA AGATGATCCC CCAGCAAGCT 
CTTGTCATCC GAGATGCAGA AAAGAAGATC ATTCCAGCAG AGCAGCTGGT GGTGGGGGAC 
GTTGTGGAGA TTAAAGGAGG GGACCAGATC CCTGCAGACA TCAGACTGGT GTTTTCCCAA 
GGGTGCAAAG TAGATAACTC CTCTCTCACT GGGGAGTCTG AGCCCCAGGC TCGGTCAACT 
GAGTTTACCC ATGAAAACCC CTTGGAAACT AAGAACATAG GCTTCTATTC TACAACGTGC 
CTGGAAGGTA CGGCAACTGG CATTGTCATC AACACGGGTG ACCGCACCAT CATCGGGCGC 
ATAGCCTCCT TGGCTTCAGG CGTTGGGAGT GAGAAGACAC CCATAGCCAT TGAGATTGAG 
CATTTTGTCC ATATTGTGGC AGCAGTGGCT GTGTCCGTTG GCGTCATTTT CTTCATCACC 
GCCGTGTGCA TGAAGTACTA CGTCCTGGAT GCCATCATCT TCCTCATTAG CATCATTGTG 
GCCAATGTGC CAGAGGGCCT CCTGGCTACT GTCACTGTTA CCCTGTCACT GACGGCAAAG 
CGGATGGCTA AGAAGAACTG CTTGGTAAAG AACCTGGAGG CCGTGGAGAC GCTTGGCTCC 
ACCTCCATCA TCTGCTCAGA TAAGACAGGG ACCCTCACCC AGAACAGGAT GACTGTGGCC 
CACTTGTGGT TTGACAACCA GATCTTTGTG GCTGATACAA GTGAAAACCA GACAAAACAA 
GCCTTTGACC AAAGCTCTGG AACCTGGGCT TCCTTGTCCA AGATAATAAC ACTGTGTAAC 
CGAGCAGAGT TCAGGCCAGG CCAGGAGAGT GTCCCCATCA TGAAGAGAGT TGTGGTTGGA 
GATGCCTCAG AAACTGCTCT GCTAAAGTTC TCAGAGGTTA TTTTGGGTGA TGTGATGGAC 
ATTAGGAAAA GAAACCACAA AGTAGCTGAG ATTCCTTTTA ATTCTACCAA CAAATTTCAG 
CTCTCCATAC ATGAGACCGA GGACCCGAAT GACAAGCGCT TCCTCATGGT GATGAAAGGG 
GCCCCTGAGA GGATCTTAGA GAAGTGCAGC ACCATCATGA TCAACGGCCA GGAGCAGCCA 
CTGGACAAGA GCTCTGCCGA TGCCTTCCAC ACGGCCTACA TGGAGCTGGG TGGTCTGGGA 
GAGCGTGTGT TGGGTTTCTG TCACCTCTAC TTGCCAGCAG ATAAGTTTCC CCAAAGCTAC 
ACATTTGATG TAGACTCCAT AAACTTTCCC ACTTCCAATC TCTGCTTTGT GGGGCTTCTG 
TCCATGATTG ACCCTCCTCG GTCTACCGTG CCAGATGCGG TCTCCAAATG TCGGAGTGCG 
GGGATCAAGG TTATCATGGT CACAGGTGAT CACCCTATCA CAGCCAAAGC TATTGCCAAG 
AGTGTAGGAA TCATATCAGC CAACAATGAG ACGGTGGAAG ACATCGCAAA ACGCCGCAAC 
ATTGCTGTGG AGCAAGTCAA CAAAAGAGAA GCGAAAGCCG CTGTGGTGAC TGGCATGGAA 
CTGAAGGACA TGACCCCAGA ACAACTGGAT GAGCTTCTAA TCAACTACCA AGAAATTGTG 
TTTGCCCGGA CATCACCCCA GCAGAAGTTG ATCATTGTAG AAGGCTGTCA GAGGCAGGAT 
GCAGTTGTTG CTGTGACGGG TGATGGAGTA AACGACTCTC CAGCATTAAA GAAGGCAGAC 
ATCGGGATTG CTATGGGGAT AGCAGGTTCT GATGCTGCTA AGAATGCAGC TGACATGGTC 
TTGCTCGATG ACAACTTTGC ATCTATAGTC ACAGGGGTGG AGGAAGGTCG CCTGATCTTC 
GACAACCTGA AGAAGACCAT TGCTTACACC CTGACAAAGA ACATCGCTGA GCTCTGCCCT 
TTTTTGATCT ACATTGTTGC TGGGCTCCCC CTGCCTATTG GCACCATCAC TATCCTATTC 
ATCGACCTGG GCACAGACAT TATCCCCTCC ATTGCCTTGG CCTATGAGAA AGCGGAAAGT 
GACATTATGA ATAGGAAACC TCGCCACAAG AAGAAAGACA GACTGGTGAA CAAGCAGCTG 
GCCATCTACT CTTACCTTCA CATTGGTCTC ATGCAAGCCC TGGGAGGTTT CCTCGTCTAT 
TTCACTGTCT ATGCACAGCA GGGCTTTTGG CCCACCTCTC TCATCAACCT GAGGGTATCA 
TGGGAAACAG ATGACATAAA TGACTTGGAA GATAGCTACG GGCAGGAATG GACGAGGTAT 
CAGAGAAAAT ACCTGGAATG GACAGGCTCA ACGGCTTTCT TTGTTGCCAT CATGGTCCAA 
CAAATTGCAG ATCTGATCAT CAGGAAAACC CGGAGGAATT CCATCTTCCA GCAGGGGCTC 
TTCAGAAACA AAGTCATCTG GGTGGGGATC ATCTCGCAGA TCATCGTTGC GTTGGTCCTC 
TCCTACGGTC TCGGGAGTGT CACAGCTCTG AGCTTTACCA TGCTCAGGGC TCAGTACTGG 
TTTGTGGCTG TCCCGCATGC CATTTTGATT TGGGTGTATG ACGAGATGCG GAAGTTGTTC 
ATCAGGCTCT ACCCTGGAAG CTGGTGGGAC AAGAACATGT ATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103553.1
CDS	344..3451
Translation	MRRKTEIYSVELNGTKDVELADQKDDKKFKGGKNKDSEPNKSQEEELKKELDLDDHRLSNTDLEQKYGTNIIQGLSSIRAAELLARDGPNALTPPKQTPEIIKFLKQMVGGFSILLWIGAALCWIAYVIQYVSSTASLDNVYLGAILVLVVILTGIFAYYQEAKSTNIMASFSKMIPQQALVIRDAEKKIIPAEQLVVGDVVEIKGGDQIPADIRLVFSQGCKVDNSSLTGESEPQARSTEFTHENPLETKNIGFYSTTCLEGTATGIVINTGDRTIIGRIASLASGVGSEKTPIAIEIEHFVHIVAAVAVSVGVIFFITAVCMKYYVLDAIIFLISIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQESVPIMKRVVVGDASETALLKFSEVILGDVMDIRKRNHKVAEIPFNSTNKFQLSIHETEDPNDKRFLMVMKGAPERILEKCSTIMINGQEQPLDKSSADAFHTAYMELGGLGERVLGFCHLYLPADKFPQSYTFDVDSINFPTSNLCFVGLLSMIDPPRSTVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVNKREAKAAVVTGMELKDMTPEQLDELLINYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAELCPFLIYIVAGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKDRLVNKQLAIYSYLHIGLMQALGGFLVYFTVYAQQGFWPTSLINLRVSWETDDINDLEDSYGQEWTRYQRKYLEWTGSTAFFVAIMVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGIISQIIVALVLSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEMRKLFIRLYPGSWWDKNMYY

Target ORF information:

RefSeq Version	XM_030247693.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247693.1

ORF Insert Sequence:

ATGCGCCGGA AAACAGAAAT CTATTCTGTA GAGCTCAATG GAACTAAAGA CGTCGAGCTG 
GCAGACCAGA AAGATGACAA AAAGTTCAAG GGCGGGAAGA ACAAAGACTC AGAACCCAAC 
AAGAGTCAGG AGGAGGAGCT GAAGAAAGAA CTTGATCTGG ATGACCACAG ACTCAGTAAT 
ACAGATTTGG AACAGAAATA TGGTACAAAC ATCATCCAGG GTCTCTCCAG TATCAGAGCT 
GCAGAGCTCC TGGCTCGGGA TGGGCCCAAC GCCCTCACCC CTCCCAAGCA AACTCCAGAG 
ATCATCAAGT TCCTCAAACA GATGGTGGGG GGCTTTTCCA TCCTTCTGTG GATAGGTGCA 
GCCTTGTGCT GGATCGCATA TGTGATCCAG TATGTCAGCA GTACTGCATC CCTGGACAAT 
GTCTATTTGG GCGCCATACT TGTCCTGGTC GTTATTTTAA CGGGGATTTT TGCTTATTAC 
CAAGAAGCCA AAAGTACCAA CATTATGGCA AGCTTTAGTA AGATGATCCC CCAGCAAGCT 
CTTGTCATCC GAGATGCAGA AAAGAAGATC ATTCCAGCAG AGCAGCTGGT GGTGGGGGAC 
GTTGTGGAGA TTAAAGGAGG GGACCAGATC CCTGCAGACA TCAGACTGGT GTTTTCCCAA 
GGGTGCAAAG TAGATAACTC CTCTCTCACT GGGGAGTCTG AGCCCCAGGC TCGGTCAACT 
GAGTTTACCC ATGAAAACCC CTTGGAAACT AAGAACATAG GCTTCTATTC TACAACGTGC 
CTGGAAGGTA CGGCAACTGG CATTGTCATC AACACGGGTG ACCGCACCAT CATCGGGCGC 
ATAGCCTCCT TGGCTTCAGG CGTTGGGAGT GAGAAGACAC CCATAGCCAT TGAGATTGAG 
CATTTTGTCC ATATTGTGGC AGCAGTGGCT GTGTCCGTTG GCGTCATTTT CTTCATCACC 
GCCGTGTGCA TGAAGTACTA CGTCCTGGAT GCCATCATCT TCCTCATTAG CATCATTGTG 
GCCAATGTGC CAGAGGGCCT CCTGGCTACT GTCACTGTTA CCCTGTCACT GACGGCAAAG 
CGGATGGCTA AGAAGAACTG CTTGGTAAAG AACCTGGAGG CCGTGGAGAC GCTTGGCTCC 
ACCTCCATCA TCTGCTCAGA TAAGACAGGG ACCCTCACCC AGAACAGGAT GACTGTGGCC 
CACTTGTGGT TTGACAACCA GATCTTTGTG GCTGATACAA GTGAAAACCA GACAAAACAA 
GCCTTTGACC AAAGCTCTGG AACCTGGGCT TCCTTGTCCA AGATAATAAC ACTGTGTAAC 
CGAGCAGAGT TCAGGCCAGG CCAGGAGAGT GTCCCCATCA TGAAGAGAGT TGTGGTTGGA 
GATGCCTCAG AAACTGCTCT GCTAAAGTTC TCAGAGGTTA TTTTGGGTGA TGTGATGGAC 
ATTAGGAAAA GAAACCACAA AGTAGCTGAG ATTCCTTTTA ATTCTACCAA CAAATTTCAG 
CTCTCCATAC ATGAGACCGA GGACCCGAAT GACAAGCGCT TCCTCATGGT GATGAAAGGG 
GCCCCTGAGA GGATCTTAGA GAAGTGCAGC ACCATCATGA TCAACGGCCA GGAGCAGCCA 
CTGGACAAGA GCTCTGCCGA TGCCTTCCAC ACGGCCTACA TGGAGCTGGG TGGTCTGGGA 
GAGCGTGTGT TGGGTTTCTG TCACCTCTAC TTGCCAGCAG ATAAGTTTCC CCAAAGCTAC 
ACATTTGATG TAGACTCCAT AAACTTTCCC ACTTCCAATC TCTGCTTTGT GGGGCTTCTG 
TCCATGATTG ACCCTCCTCG GTCTACCGTG CCAGATGCGG TCTCCAAATG TCGGAGTGCG 
GGGATCAAGG TTATCATGGT CACAGGTGAT CACCCTATCA CAGCCAAAGC TATTGCCAAG 
AGTGTAGGAA TCATATCAGC CAACAATGAG ACGGTGGAAG ACATCGCAAA ACGCCGCAAC 
ATTGCTGTGG AGCAAGTCAA CAAAAGAGAA GCGAAAGCCG CTGTGGTGAC TGGCATGGAA 
CTGAAGGACA TGACCCCAGA ACAACTGGAT GAGCTTCTAA TCAACTACCA AGAAATTGTG 
TTTGCCCGGA CATCACCCCA GCAGAAGTTG ATCATTGTAG AAGGCTGTCA GAGGCAGGAT 
GCAGTTGTTG CTGTGACGGG TGATGGAGTA AACGACTCTC CAGCATTAAA GAAGGCAGAC 
ATCGGGATTG CTATGGGGAT AGCAGGTTCT GATGCTGCTA AGAATGCAGC TGACATGGTC 
TTGCTCGATG ACAACTTTGC ATCTATAGTC ACAGGGGTGG AGGAAGGTCG CCTGATCTTC 
GACAACCTGA AGAAGACCAT TGCTTACACC CTGACAAAGA ACATCGCTGA GCTCTGCCCT 
TTTTTGATCT ACATTGTTGC TGGGCTCCCC CTGCCTATTG GCACCATCAC TATCCTATTC 
ATCGACCTGG GCACAGACAT TATCCCCTCC ATTGCCTTGG CCTATGAGAA AGCGGAAAGT 
GACATTATGA ATAGGAAACC TCGCCACAAG AAGAAAGACA GACTGGTGAA CAAGCAGCTG 
GCCATCTACT CTTACCTTCA CATTGGTCTC ATGCAAGCCC TGGGAGGTTT CCTCGTCTAT 
TTCACTGTCT ATGCACAGCA GGGCTTTTGG CCCACCTCTC TCATCAACCT GAGGGTATCA 
TGGGAAACAG ATGACATAAA TGACTTGGAA GATAGCTACG GGCAGGAATG GACGAGGTAT 
CAGAGAAAAT ACCTGGAATG GACAGGCTCA ACGGCTTTCT TTGTTGCCAT CATGGTCCAA 
CAAATTGCAG ATCTGATCAT CAGGAAAACC CGGAGGAATT CCATCTTCCA GCAGGGGCTC 
TTCAGAAACA AAGTCATCTG GGTGGGGATC ATCTCGCAGA TCATCGTTGC GTTGGTCCTC 
TCCTACGGTC TCGGGAGTGT CACAGCTCTG AGCTTTACCA TGCTCAGGGC TCAGTACTGG 
TTTGTGGCTG TCCCGCATGC CATTTTGATT TGGGTGTATG ACGAGATGCG GAAGTTGTTC 
ATCAGGCTCT ACCCTGGAAG CTGGTGGGAC AAGAACATGT ATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction.
Pflugers Archiv : European journal of physiology468(10)1673-83(2016 Oct)
Walter C,Tanfous MB,Igoudjil K,Salhi A,Escher G,Crambert G

A link between fertility and K+ homeostasis: role of the renal H,K-ATPase type 2.
Pflugers Archiv : European journal of physiology465(8)1149-58(2013 Aug)
Salhi A,Lamouroux C,Pestov NB,Modyanov NN,Doucet A,Crambert G

Circadian expression of H,K-ATPase type 2 contributes to the stability of plasma K? levels.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(7)2859-67(2012 Jul)
Salhi A,Centeno G,Firsov D,Crambert G

Mineralocorticoids stimulate the activity and expression of renal H+,K+-ATPases.
Journal of the American Society of Nephrology : JASN22(1)49-58(2011 Jan)
Greenlee MM,Lynch IJ,Gumz ML,Cain BD,Wingo CS

Sp1 trans-activates the murine H(+)-K(+)-ATPase alpha(2)-subunit gene.
American journal of physiology. Renal physiology297(1)F63-70(2009 Jul)
Yu Z,Li M,Zhang D,Xu W,Kone BC

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Atp12a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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