Kcnj12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnj12
Entrez Gene ID	16515
Full Name	potassium inwardly-rectifying channel, subfamily J, member 12
Synonyms	IRK-2,IRK2,Kir2.2,MB-IRK2
General protein information	Preferred Names potassium inwardly-rectifying channel, subfamily J, member 12 Names ATP-sensitive inward rectifier potassium channel 12 inward rectifier K(+) channel Kir2.2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B2\|11 37.96 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030245606.1	XP_030101466.1	ATP-sensitive inward rectifier potassium channel 12 isoform X1
XM_006532318.3	XP_006532381.1	ATP-sensitive inward rectifier potassium channel 12 isoform X1
NM_001267593.1	NP_001254522.1	ATP-sensitive inward rectifier potassium channel 12 isoform 2
XM_006532318.4	XP_006532381.1	ATP-sensitive inward rectifier potassium channel 12 isoform X2
NM_010603.6	NP_034733.4	ATP-sensitive inward rectifier potassium channel 12 isoform 1

Pathways from BioSystems

Homologies

	KCNJ12	XP_002800376.1
Danio rerio (zebrafish)	LOC569146	XP_005162964.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001281	XP_001689370.2
Pan troglodytes (chimpanzee)	KCNJ12	XP_001154349.1
Caenorhabditis elegans (roundworm)	irk-2	NP_001256977.1
Xenopus tropicalis (tropical clawed frog)	LOC100496092	XP_004917900.1
Homo sapiens (human)	KCNJ12	NP_066292.2
Canis lupus familiaris (dog)	KCNJ12	NP_001229643.1
Rattus norvegicus (Norway rat)	Kcnj12	NP_446433.2
Bos taurus (cattle)	KCNJ12	NP_001019861.2
Mus musculus (house mouse)	Kcnj12	NP_034733.4
Gallus gallus (chicken)	KCNJ12	XP_004945226.1
Drosophila melanogaster (fruit fly)	Irk2	NP_001163700.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001283	XP_003435798.1

Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

PITX2 insufficiency leads to atrial electrical and structural remodeling linked to arrhythmogenesis.
Chinchilla A, Daimi H, Lozano-Velasco E, Dominguez JN, Caballero R, Delp?n E, Tamargo J, Cinca J, Hove-Madsen L, Aranega AE, Franco D
Circulation. Cardiovascular genetics4(3)269-79(2011 Jun)

Functional characterization of inward rectifier potassium ion channel in murine fetal ventricular cardiomyocytes.
Liu A, Tang M, Xi J, Gao L, Zheng Y, Luo H, Hu X, Zhao F, Reppel M, Hescheler J, Liang H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology26(3)413-20(2010)

Low-affinity spermine block mediating outward currents through Kir2.1 and Kir2.2 inward rectifier potassium channels.
Ishihara K, Yan DH
The Journal of physiology583(Pt 3)891-908(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kir2 channels exist in two states having different susceptibilities to internal cationic blockers
Title: Low-affinity spermine block mediating outward currents through Kir2.1 and Kir2.2 inward rectifier potassium channels.

Computer simulations grounded in biophysical measurements of prelimbic and infralimbic cortex slices suggest a dynamic interaction among Kir2, Kleak, and HCN channel currents in shaping membrane potential and temporal integration of synaptic potentials.
Title: Dendritic excitability of mouse frontal cortex pyramidal neurons is shaped by the interaction among HCN, Kir2, and Kleak channels.

Kir2.2 in the brainstem plays a transient role in hypercapnic ventilatory response , possibly through central ventilatory chemosensitivity, during postnatal development.
Title: Role of Kir2.2 in hypercapnic ventilatory response during postnatal development of mouse.

The following Kcnj12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnj12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06438	NM_010603.6 Latest version!	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu06637	NM_001267593.1 Latest version!	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu06637	XM_006532318.4 Latest version!	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu06438	XM_030245606.1 Latest version!	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu06637	XM_006532318.3	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnj12 ( NM_010603.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034733.4 Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06438
Clone ID Related Accession (Same CDS sequence)	XM_030245606.1 , NM_010603.6
Accession Version	NM_010603.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	ATP-sensitive inward rectifier potassium channel 12 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK017299.1 and AL646093.9. On Jun 5, 2012 this sequence version replaced NM_010603.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK017299.1, BY717111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGACCCGCT GGACGTCCCT CAAGGCCTCT AGGCCACACA AGCCTGGCTC CTGGGATCTG 
GCAGCTTCCC CCCGGCGTCT GAGTACCCCA ACCTTGGCCA CCTTCCCCTG GAAGGCTCAG 
GAACCCGGGG CTGAGATGTC TTCTCAGAAT GCTCCCCGGG TTCCCCAGCC CCGTCAACCC 
TCTGCCTCCT GGCCTAGACA GAAACAGCAT CCACCATTAC AGCCCCAAGC CTCAGCTAAA 
CAAGCCCATG AAGTGCCCCC GCCTGGAGTC AGCCTGGGGG CAGGCCAGGG TCCACCAGAC 
CCCGGGATGA CCGCAGCCAG TCGGGCCAAC CCCTACAGCA TCGTATCATC AGAGGAGGAC 
GGGCTGCACC TGGTTACCAT GTCAGGCGCC AACGGTTTTG GCAATGGCAA GGTGCATACA 
CGGCGCCGGT GCCGCAACCG CTTCGTCAAG AAGAACGGTC AGTGCAACAT TGAATTCGCC 
AACATGGACG AGAAGTCACA ACGCTACCTG GCTGACATGT TTACCACGTG TGTGGACATC 
CGCTGGCGCT ACATGCTGCT CATCTTCTCT CTGGCCTTTC TTGCCTCCTG GTTGTTGTTT 
GGCATCATCT TCTGGGTCAT TGCTGTCGCC CACGGGGACC TGGAGCCAGC CGAGGGCCGT 
GGCCGTACAC CCTGTGTGCT GCAGGTCCAC GGCTTCATGG CAGCCTTTCT CTTCTCCATT 
GAGACACAGA CCACCATTGG CTACGGGCTA CGCTGTGTGA CTGAAGAGTG CCCGGTGGCT 
GTCTTCATGG TGGTGGCGCA GTCCATTGTG GGCTGCATCA TTGACTCCTT CATGATTGGT 
GCCATCATGG CCAAGATGGC ACGGCCCAAG AAGCGCGCAC AGACTCTGCT TTTCAGCCAT 
AATGCCGTGG TGGCTCTGCG TGACGGCAAG CTCTGCCTCA TGTGGCGCGT GGGCAACCTG 
CGTAAGAGTC ACATCGTGGA GGCCCATGTG CGGGCCCAGC TCATCAAGCC CAGGGTCACA 
GAGGAGGGTG AGTACATCCC ACTGGACCAG ATTGACATCG ATGTCGGCTT TGACAAGGGC 
CTAGACCGTA TCTTCCTGGT ATCACCCATC ACCATCTTGC ACGAGATTGA TGAGGCCAGC 
CCACTGTTTG GCATTAGCCG TCAGGACCTT GAGACAGACG ACTTTGAGAT TGTGGTCATC 
CTGGAGGGCA TGGTAGAGGC CACAGCCATG ACCACACAGG CTCGCAGTTC CTACCTGGCT 
AACGAGATCC TGTGGGGCCA CCGCTTTGAG CCAGTGCTCT TCGAAGAGAA GAACCAGTAC 
AAGATTGACT ATTCACACTT CCACAAGACC TACGAGGTGC CATCTACACC CCGCTGCAGC 
GCCAAGGACC TGGTGGAGAA CAAGTTCCTC CTGCCCAGCG CCAACTCTTT CTGCTATGAG 
AACGAGCTGG CCTTCCTGAG CAGAGATGAG GAGGACGAGG TGGCTACCGA CCGGGATGGC 
CGCAGCCCTC AGCCCGAGCA TGACTTTGAC AGACTGCAGG CCAGCAGCGC TGCCCTTGAA 
CGGCCCTACA GACGGGAGTC GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034733.4
CDS	210..1799
Translation	MTRWTSLKASRPHKPGSWDLAASPRRLSTPTLATFPWKAQEPGAEMSSQNAPRVPQPRQPSASWPRQKQHPPLQPQASAKQAHEVPPPGVSLGAGQGPPDPGMTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVLQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEVATDRDGRSPQPEHDFDRLQASSAALERPYRRESEI

Target ORF information:

RefSeq Version	NM_010603.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010603.6

ORF Insert Sequence:

ATGACCCGCT GGACGTCCCT CAAGGCCTCT AGGCCACACA AGCCTGGCTC CTGGGATCTG 
GCAGCTTCCC CCCGGCGTCT GAGTACCCCA ACCTTGGCCA CCTTCCCCTG GAAGGCTCAG 
GAACCCGGGG CTGAGATGTC TTCTCAGAAT GCTCCCCGGG TTCCCCAGCC CCGTCAACCC 
TCTGCCTCCT GGCCTAGACA GAAACAGCAT CCACCATTAC AGCCCCAAGC CTCAGCTAAA 
CAAGCCCATG AAGTGCCCCC GCCTGGAGTC AGCCTGGGGG CAGGCCAGGG TCCACCAGAC 
CCCGGGATGA CCGCAGCCAG TCGGGCCAAC CCCTACAGCA TCGTATCATC AGAGGAGGAC 
GGGCTGCACC TGGTTACCAT GTCAGGCGCC AACGGTTTTG GCAATGGCAA GGTGCATACA 
CGGCGCCGGT GCCGCAACCG CTTCGTCAAG AAGAACGGTC AGTGCAACAT TGAATTCGCC 
AACATGGACG AGAAGTCACA ACGCTACCTG GCTGACATGT TTACCACGTG TGTGGACATC 
CGCTGGCGCT ACATGCTGCT CATCTTCTCT CTGGCCTTTC TTGCCTCCTG GTTGTTGTTT 
GGCATCATCT TCTGGGTCAT TGCTGTCGCC CACGGGGACC TGGAGCCAGC CGAGGGCCGT 
GGCCGTACAC CCTGTGTGCT GCAGGTCCAC GGCTTCATGG CAGCCTTTCT CTTCTCCATT 
GAGACACAGA CCACCATTGG CTACGGGCTA CGCTGTGTGA CTGAAGAGTG CCCGGTGGCT 
GTCTTCATGG TGGTGGCGCA GTCCATTGTG GGCTGCATCA TTGACTCCTT CATGATTGGT 
GCCATCATGG CCAAGATGGC ACGGCCCAAG AAGCGCGCAC AGACTCTGCT TTTCAGCCAT 
AATGCCGTGG TGGCTCTGCG TGACGGCAAG CTCTGCCTCA TGTGGCGCGT GGGCAACCTG 
CGTAAGAGTC ACATCGTGGA GGCCCATGTG CGGGCCCAGC TCATCAAGCC CAGGGTCACA 
GAGGAGGGTG AGTACATCCC ACTGGACCAG ATTGACATCG ATGTCGGCTT TGACAAGGGC 
CTAGACCGTA TCTTCCTGGT ATCACCCATC ACCATCTTGC ACGAGATTGA TGAGGCCAGC 
CCACTGTTTG GCATTAGCCG TCAGGACCTT GAGACAGACG ACTTTGAGAT TGTGGTCATC 
CTGGAGGGCA TGGTAGAGGC CACAGCCATG ACCACACAGG CTCGCAGTTC CTACCTGGCT 
AACGAGATCC TGTGGGGCCA CCGCTTTGAG CCAGTGCTCT TCGAAGAGAA GAACCAGTAC 
AAGATTGACT ATTCACACTT CCACAAGACC TACGAGGTGC CATCTACACC CCGCTGCAGC 
GCCAAGGACC TGGTGGAGAA CAAGTTCCTC CTGCCCAGCG CCAACTCTTT CTGCTATGAG 
AACGAGCTGG CCTTCCTGAG CAGAGATGAG GAGGACGAGG TGGCTACCGA CCGGGATGGC 
CGCAGCCCTC AGCCCGAGCA TGACTTTGAC AGACTGCAGG CCAGCAGCGC TGCCCTTGAA 
CGGCCCTACA GACGGGAGTC GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj12 ( NM_001267593.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001254522.1 Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06637
Clone ID Related Accession (Same CDS sequence)	XM_006532318.3 , NM_001267593.1 , XM_006532318.4
Accession Version	NM_001267593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ATP-sensitive inward rectifier potassium channel 12 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK032271.1 and AL646093.9. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK032271.1, BB622596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACCGCAG CCAGTCGGGC CAACCCCTAC AGCATCGTAT CATCAGAGGA GGACGGGCTG 
CACCTGGTTA CCATGTCAGG CGCCAACGGT TTTGGCAATG GCAAGGTGCA TACACGGCGC 
CGGTGCCGCA ACCGCTTCGT CAAGAAGAAC GGTCAGTGCA ACATTGAATT CGCCAACATG 
GACGAGAAGT CACAACGCTA CCTGGCTGAC ATGTTTACCA CGTGTGTGGA CATCCGCTGG 
CGCTACATGC TGCTCATCTT CTCTCTGGCC TTTCTTGCCT CCTGGTTGTT GTTTGGCATC 
ATCTTCTGGG TCATTGCTGT CGCCCACGGG GACCTGGAGC CAGCCGAGGG CCGTGGCCGT 
ACACCCTGTG TGCTGCAGGT CCACGGCTTC ATGGCAGCCT TTCTCTTCTC CATTGAGACA 
CAGACCACCA TTGGCTACGG GCTACGCTGT GTGACTGAAG AGTGCCCGGT GGCTGTCTTC 
ATGGTGGTGG CGCAGTCCAT TGTGGGCTGC ATCATTGACT CCTTCATGAT TGGTGCCATC 
ATGGCCAAGA TGGCACGGCC CAAGAAGCGC GCACAGACTC TGCTTTTCAG CCATAATGCC 
GTGGTGGCTC TGCGTGACGG CAAGCTCTGC CTCATGTGGC GCGTGGGCAA CCTGCGTAAG 
AGTCACATCG TGGAGGCCCA TGTGCGGGCC CAGCTCATCA AGCCCAGGGT CACAGAGGAG 
GGTGAGTACA TCCCACTGGA CCAGATTGAC ATCGATGTCG GCTTTGACAA GGGCCTAGAC 
CGTATCTTCC TGGTATCACC CATCACCATC TTGCACGAGA TTGATGAGGC CAGCCCACTG 
TTTGGCATTA GCCGTCAGGA CCTTGAGACA GACGACTTTG AGATTGTGGT CATCCTGGAG 
GGCATGGTAG AGGCCACAGC CATGACCACA CAGGCTCGCA GTTCCTACCT GGCTAACGAG 
ATCCTGTGGG GCCACCGCTT TGAGCCAGTG CTCTTCGAAG AGAAGAACCA GTACAAGATT 
GACTATTCAC ACTTCCACAA GACCTACGAG GTGCCATCTA CACCCCGCTG CAGCGCCAAG 
GACCTGGTGG AGAACAAGTT CCTCCTGCCC AGCGCCAACT CTTTCTGCTA TGAGAACGAG 
CTGGCCTTCC TGAGCAGAGA TGAGGAGGAC GAGGTGGCTA CCGACCGGGA TGGCCGCAGC 
CCTCAGCCCG AGCATGACTT TGACAGACTG CAGGCCAGCA GCGCTGCCCT TGAACGGCCC 
TACAGACGGG AGTCGGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254522.1
CDS	344..1627
Translation	MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVLQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEVATDRDGRSPQPEHDFDRLQASSAALERPYRRESEI

Target ORF information:

RefSeq Version	NM_001267593.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267593.1

ORF Insert Sequence:

ATGACCGCAG CCAGTCGGGC CAACCCCTAC AGCATCGTAT CATCAGAGGA GGACGGGCTG 
CACCTGGTTA CCATGTCAGG CGCCAACGGT TTTGGCAATG GCAAGGTGCA TACACGGCGC 
CGGTGCCGCA ACCGCTTCGT CAAGAAGAAC GGTCAGTGCA ACATTGAATT CGCCAACATG 
GACGAGAAGT CACAACGCTA CCTGGCTGAC ATGTTTACCA CGTGTGTGGA CATCCGCTGG 
CGCTACATGC TGCTCATCTT CTCTCTGGCC TTTCTTGCCT CCTGGTTGTT GTTTGGCATC 
ATCTTCTGGG TCATTGCTGT CGCCCACGGG GACCTGGAGC CAGCCGAGGG CCGTGGCCGT 
ACACCCTGTG TGCTGCAGGT CCACGGCTTC ATGGCAGCCT TTCTCTTCTC CATTGAGACA 
CAGACCACCA TTGGCTACGG GCTACGCTGT GTGACTGAAG AGTGCCCGGT GGCTGTCTTC 
ATGGTGGTGG CGCAGTCCAT TGTGGGCTGC ATCATTGACT CCTTCATGAT TGGTGCCATC 
ATGGCCAAGA TGGCACGGCC CAAGAAGCGC GCACAGACTC TGCTTTTCAG CCATAATGCC 
GTGGTGGCTC TGCGTGACGG CAAGCTCTGC CTCATGTGGC GCGTGGGCAA CCTGCGTAAG 
AGTCACATCG TGGAGGCCCA TGTGCGGGCC CAGCTCATCA AGCCCAGGGT CACAGAGGAG 
GGTGAGTACA TCCCACTGGA CCAGATTGAC ATCGATGTCG GCTTTGACAA GGGCCTAGAC 
CGTATCTTCC TGGTATCACC CATCACCATC TTGCACGAGA TTGATGAGGC CAGCCCACTG 
TTTGGCATTA GCCGTCAGGA CCTTGAGACA GACGACTTTG AGATTGTGGT CATCCTGGAG 
GGCATGGTAG AGGCCACAGC CATGACCACA CAGGCTCGCA GTTCCTACCT GGCTAACGAG 
ATCCTGTGGG GCCACCGCTT TGAGCCAGTG CTCTTCGAAG AGAAGAACCA GTACAAGATT 
GACTATTCAC ACTTCCACAA GACCTACGAG GTGCCATCTA CACCCCGCTG CAGCGCCAAG 
GACCTGGTGG AGAACAAGTT CCTCCTGCCC AGCGCCAACT CTTTCTGCTA TGAGAACGAG 
CTGGCCTTCC TGAGCAGAGA TGAGGAGGAC GAGGTGGCTA CCGACCGGGA TGGCCGCAGC 
CCTCAGCCCG AGCATGACTT TGACAGACTG CAGGCCAGCA GCGCTGCCCT TGAACGGCCC 
TACAGACGGG AGTCGGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj12 ( XM_006532318.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532381.1 Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu06637
Clone ID Related Accession (Same CDS sequence)	XM_006532318.3 , NM_001267593.1 , XM_006532318.4
Accession Version	XM_006532318.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ATP-sensitive inward rectifier potassium channel 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCAG CCAGTCGGGC CAACCCCTAC AGCATCGTAT CATCAGAGGA GGACGGGCTG 
CACCTGGTTA CCATGTCAGG CGCCAACGGT TTTGGCAATG GCAAGGTGCA TACACGGCGC 
CGGTGCCGCA ACCGCTTCGT CAAGAAGAAC GGTCAGTGCA ACATTGAATT CGCCAACATG 
GACGAGAAGT CACAACGCTA CCTGGCTGAC ATGTTTACCA CGTGTGTGGA CATCCGCTGG 
CGCTACATGC TGCTCATCTT CTCTCTGGCC TTTCTTGCCT CCTGGTTGTT GTTTGGCATC 
ATCTTCTGGG TCATTGCTGT CGCCCACGGG GACCTGGAGC CAGCCGAGGG CCGTGGCCGT 
ACACCCTGTG TGCTGCAGGT CCACGGCTTC ATGGCAGCCT TTCTCTTCTC CATTGAGACA 
CAGACCACCA TTGGCTACGG GCTACGCTGT GTGACTGAAG AGTGCCCGGT GGCTGTCTTC 
ATGGTGGTGG CGCAGTCCAT TGTGGGCTGC ATCATTGACT CCTTCATGAT TGGTGCCATC 
ATGGCCAAGA TGGCACGGCC CAAGAAGCGC GCACAGACTC TGCTTTTCAG CCATAATGCC 
GTGGTGGCTC TGCGTGACGG CAAGCTCTGC CTCATGTGGC GCGTGGGCAA CCTGCGTAAG 
AGTCACATCG TGGAGGCCCA TGTGCGGGCC CAGCTCATCA AGCCCAGGGT CACAGAGGAG 
GGTGAGTACA TCCCACTGGA CCAGATTGAC ATCGATGTCG GCTTTGACAA GGGCCTAGAC 
CGTATCTTCC TGGTATCACC CATCACCATC TTGCACGAGA TTGATGAGGC CAGCCCACTG 
TTTGGCATTA GCCGTCAGGA CCTTGAGACA GACGACTTTG AGATTGTGGT CATCCTGGAG 
GGCATGGTAG AGGCCACAGC CATGACCACA CAGGCTCGCA GTTCCTACCT GGCTAACGAG 
ATCCTGTGGG GCCACCGCTT TGAGCCAGTG CTCTTCGAAG AGAAGAACCA GTACAAGATT 
GACTATTCAC ACTTCCACAA GACCTACGAG GTGCCATCTA CACCCCGCTG CAGCGCCAAG 
GACCTGGTGG AGAACAAGTT CCTCCTGCCC AGCGCCAACT CTTTCTGCTA TGAGAACGAG 
CTGGCCTTCC TGAGCAGAGA TGAGGAGGAC GAGGTGGCTA CCGACCGGGA TGGCCGCAGC 
CCTCAGCCCG AGCATGACTT TGACAGACTG CAGGCCAGCA GCGCTGCCCT TGAACGGCCC 
TACAGACGGG AGTCGGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532381.1
CDS	77..1360
Translation	MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVLQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEVATDRDGRSPQPEHDFDRLQASSAALERPYRRESEI

Target ORF information:

RefSeq Version	XM_006532318.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532318.3

ORF Insert Sequence:

ATGACCGCAG CCAGTCGGGC CAACCCCTAC AGCATCGTAT CATCAGAGGA GGACGGGCTG 
CACCTGGTTA CCATGTCAGG CGCCAACGGT TTTGGCAATG GCAAGGTGCA TACACGGCGC 
CGGTGCCGCA ACCGCTTCGT CAAGAAGAAC GGTCAGTGCA ACATTGAATT CGCCAACATG 
GACGAGAAGT CACAACGCTA CCTGGCTGAC ATGTTTACCA CGTGTGTGGA CATCCGCTGG 
CGCTACATGC TGCTCATCTT CTCTCTGGCC TTTCTTGCCT CCTGGTTGTT GTTTGGCATC 
ATCTTCTGGG TCATTGCTGT CGCCCACGGG GACCTGGAGC CAGCCGAGGG CCGTGGCCGT 
ACACCCTGTG TGCTGCAGGT CCACGGCTTC ATGGCAGCCT TTCTCTTCTC CATTGAGACA 
CAGACCACCA TTGGCTACGG GCTACGCTGT GTGACTGAAG AGTGCCCGGT GGCTGTCTTC 
ATGGTGGTGG CGCAGTCCAT TGTGGGCTGC ATCATTGACT CCTTCATGAT TGGTGCCATC 
ATGGCCAAGA TGGCACGGCC CAAGAAGCGC GCACAGACTC TGCTTTTCAG CCATAATGCC 
GTGGTGGCTC TGCGTGACGG CAAGCTCTGC CTCATGTGGC GCGTGGGCAA CCTGCGTAAG 
AGTCACATCG TGGAGGCCCA TGTGCGGGCC CAGCTCATCA AGCCCAGGGT CACAGAGGAG 
GGTGAGTACA TCCCACTGGA CCAGATTGAC ATCGATGTCG GCTTTGACAA GGGCCTAGAC 
CGTATCTTCC TGGTATCACC CATCACCATC TTGCACGAGA TTGATGAGGC CAGCCCACTG 
TTTGGCATTA GCCGTCAGGA CCTTGAGACA GACGACTTTG AGATTGTGGT CATCCTGGAG 
GGCATGGTAG AGGCCACAGC CATGACCACA CAGGCTCGCA GTTCCTACCT GGCTAACGAG 
ATCCTGTGGG GCCACCGCTT TGAGCCAGTG CTCTTCGAAG AGAAGAACCA GTACAAGATT 
GACTATTCAC ACTTCCACAA GACCTACGAG GTGCCATCTA CACCCCGCTG CAGCGCCAAG 
GACCTGGTGG AGAACAAGTT CCTCCTGCCC AGCGCCAACT CTTTCTGCTA TGAGAACGAG 
CTGGCCTTCC TGAGCAGAGA TGAGGAGGAC GAGGTGGCTA CCGACCGGGA TGGCCGCAGC 
CCTCAGCCCG AGCATGACTT TGACAGACTG CAGGCCAGCA GCGCTGCCCT TGAACGGCCC 
TACAGACGGG AGTCGGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj12 ( XM_006532318.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532381.1 Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X2, mRNA.

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CloneID	OMu06637
Clone ID Related Accession (Same CDS sequence)	XM_006532318.3 , NM_001267593.1 , XM_006532318.4
Accession Version	XM_006532318.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-sensitive inward rectifier potassium channel 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532318.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCAG CCAGTCGGGC CAACCCCTAC AGCATCGTAT CATCAGAGGA GGACGGGCTG 
CACCTGGTTA CCATGTCAGG CGCCAACGGT TTTGGCAATG GCAAGGTGCA TACACGGCGC 
CGGTGCCGCA ACCGCTTCGT CAAGAAGAAC GGTCAGTGCA ACATTGAATT CGCCAACATG 
GACGAGAAGT CACAACGCTA CCTGGCTGAC ATGTTTACCA CGTGTGTGGA CATCCGCTGG 
CGCTACATGC TGCTCATCTT CTCTCTGGCC TTTCTTGCCT CCTGGTTGTT GTTTGGCATC 
ATCTTCTGGG TCATTGCTGT CGCCCACGGG GACCTGGAGC CAGCCGAGGG CCGTGGCCGT 
ACACCCTGTG TGCTGCAGGT CCACGGCTTC ATGGCAGCCT TTCTCTTCTC CATTGAGACA 
CAGACCACCA TTGGCTACGG GCTACGCTGT GTGACTGAAG AGTGCCCGGT GGCTGTCTTC 
ATGGTGGTGG CGCAGTCCAT TGTGGGCTGC ATCATTGACT CCTTCATGAT TGGTGCCATC 
ATGGCCAAGA TGGCACGGCC CAAGAAGCGC GCACAGACTC TGCTTTTCAG CCATAATGCC 
GTGGTGGCTC TGCGTGACGG CAAGCTCTGC CTCATGTGGC GCGTGGGCAA CCTGCGTAAG 
AGTCACATCG TGGAGGCCCA TGTGCGGGCC CAGCTCATCA AGCCCAGGGT CACAGAGGAG 
GGTGAGTACA TCCCACTGGA CCAGATTGAC ATCGATGTCG GCTTTGACAA GGGCCTAGAC 
CGTATCTTCC TGGTATCACC CATCACCATC TTGCACGAGA TTGATGAGGC CAGCCCACTG 
TTTGGCATTA GCCGTCAGGA CCTTGAGACA GACGACTTTG AGATTGTGGT CATCCTGGAG 
GGCATGGTAG AGGCCACAGC CATGACCACA CAGGCTCGCA GTTCCTACCT GGCTAACGAG 
ATCCTGTGGG GCCACCGCTT TGAGCCAGTG CTCTTCGAAG AGAAGAACCA GTACAAGATT 
GACTATTCAC ACTTCCACAA GACCTACGAG GTGCCATCTA CACCCCGCTG CAGCGCCAAG 
GACCTGGTGG AGAACAAGTT CCTCCTGCCC AGCGCCAACT CTTTCTGCTA TGAGAACGAG 
CTGGCCTTCC TGAGCAGAGA TGAGGAGGAC GAGGTGGCTA CCGACCGGGA TGGCCGCAGC 
CCTCAGCCCG AGCATGACTT TGACAGACTG CAGGCCAGCA GCGCTGCCCT TGAACGGCCC 
TACAGACGGG AGTCGGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532381.1
CDS	78..1361
Translation	MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVLQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEVATDRDGRSPQPEHDFDRLQASSAALERPYRRESEI

Target ORF information:

RefSeq Version	XM_006532318.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532318.4

ORF Insert Sequence:

ATGACCGCAG CCAGTCGGGC CAACCCCTAC AGCATCGTAT CATCAGAGGA GGACGGGCTG 
CACCTGGTTA CCATGTCAGG CGCCAACGGT TTTGGCAATG GCAAGGTGCA TACACGGCGC 
CGGTGCCGCA ACCGCTTCGT CAAGAAGAAC GGTCAGTGCA ACATTGAATT CGCCAACATG 
GACGAGAAGT CACAACGCTA CCTGGCTGAC ATGTTTACCA CGTGTGTGGA CATCCGCTGG 
CGCTACATGC TGCTCATCTT CTCTCTGGCC TTTCTTGCCT CCTGGTTGTT GTTTGGCATC 
ATCTTCTGGG TCATTGCTGT CGCCCACGGG GACCTGGAGC CAGCCGAGGG CCGTGGCCGT 
ACACCCTGTG TGCTGCAGGT CCACGGCTTC ATGGCAGCCT TTCTCTTCTC CATTGAGACA 
CAGACCACCA TTGGCTACGG GCTACGCTGT GTGACTGAAG AGTGCCCGGT GGCTGTCTTC 
ATGGTGGTGG CGCAGTCCAT TGTGGGCTGC ATCATTGACT CCTTCATGAT TGGTGCCATC 
ATGGCCAAGA TGGCACGGCC CAAGAAGCGC GCACAGACTC TGCTTTTCAG CCATAATGCC 
GTGGTGGCTC TGCGTGACGG CAAGCTCTGC CTCATGTGGC GCGTGGGCAA CCTGCGTAAG 
AGTCACATCG TGGAGGCCCA TGTGCGGGCC CAGCTCATCA AGCCCAGGGT CACAGAGGAG 
GGTGAGTACA TCCCACTGGA CCAGATTGAC ATCGATGTCG GCTTTGACAA GGGCCTAGAC 
CGTATCTTCC TGGTATCACC CATCACCATC TTGCACGAGA TTGATGAGGC CAGCCCACTG 
TTTGGCATTA GCCGTCAGGA CCTTGAGACA GACGACTTTG AGATTGTGGT CATCCTGGAG 
GGCATGGTAG AGGCCACAGC CATGACCACA CAGGCTCGCA GTTCCTACCT GGCTAACGAG 
ATCCTGTGGG GCCACCGCTT TGAGCCAGTG CTCTTCGAAG AGAAGAACCA GTACAAGATT 
GACTATTCAC ACTTCCACAA GACCTACGAG GTGCCATCTA CACCCCGCTG CAGCGCCAAG 
GACCTGGTGG AGAACAAGTT CCTCCTGCCC AGCGCCAACT CTTTCTGCTA TGAGAACGAG 
CTGGCCTTCC TGAGCAGAGA TGAGGAGGAC GAGGTGGCTA CCGACCGGGA TGGCCGCAGC 
CCTCAGCCCG AGCATGACTT TGACAGACTG CAGGCCAGCA GCGCTGCCCT TGAACGGCCC 
TACAGACGGG AGTCGGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj12 ( XM_030245606.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101466.1 Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu06438
Clone ID Related Accession (Same CDS sequence)	XM_030245606.1 , NM_010603.6
Accession Version	XM_030245606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-sensitive inward rectifier potassium channel 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGCT GGACGTCCCT CAAGGCCTCT AGGCCACACA AGCCTGGCTC CTGGGATCTG 
GCAGCTTCCC CCCGGCGTCT GAGTACCCCA ACCTTGGCCA CCTTCCCCTG GAAGGCTCAG 
GAACCCGGGG CTGAGATGTC TTCTCAGAAT GCTCCCCGGG TTCCCCAGCC CCGTCAACCC 
TCTGCCTCCT GGCCTAGACA GAAACAGCAT CCACCATTAC AGCCCCAAGC CTCAGCTAAA 
CAAGCCCATG AAGTGCCCCC GCCTGGAGTC AGCCTGGGGG CAGGCCAGGG TCCACCAGAC 
CCCGGGATGA CCGCAGCCAG TCGGGCCAAC CCCTACAGCA TCGTATCATC AGAGGAGGAC 
GGGCTGCACC TGGTTACCAT GTCAGGCGCC AACGGTTTTG GCAATGGCAA GGTGCATACA 
CGGCGCCGGT GCCGCAACCG CTTCGTCAAG AAGAACGGTC AGTGCAACAT TGAATTCGCC 
AACATGGACG AGAAGTCACA ACGCTACCTG GCTGACATGT TTACCACGTG TGTGGACATC 
CGCTGGCGCT ACATGCTGCT CATCTTCTCT CTGGCCTTTC TTGCCTCCTG GTTGTTGTTT 
GGCATCATCT TCTGGGTCAT TGCTGTCGCC CACGGGGACC TGGAGCCAGC CGAGGGCCGT 
GGCCGTACAC CCTGTGTGCT GCAGGTCCAC GGCTTCATGG CAGCCTTTCT CTTCTCCATT 
GAGACACAGA CCACCATTGG CTACGGGCTA CGCTGTGTGA CTGAAGAGTG CCCGGTGGCT 
GTCTTCATGG TGGTGGCGCA GTCCATTGTG GGCTGCATCA TTGACTCCTT CATGATTGGT 
GCCATCATGG CCAAGATGGC ACGGCCCAAG AAGCGCGCAC AGACTCTGCT TTTCAGCCAT 
AATGCCGTGG TGGCTCTGCG TGACGGCAAG CTCTGCCTCA TGTGGCGCGT GGGCAACCTG 
CGTAAGAGTC ACATCGTGGA GGCCCATGTG CGGGCCCAGC TCATCAAGCC CAGGGTCACA 
GAGGAGGGTG AGTACATCCC ACTGGACCAG ATTGACATCG ATGTCGGCTT TGACAAGGGC 
CTAGACCGTA TCTTCCTGGT ATCACCCATC ACCATCTTGC ACGAGATTGA TGAGGCCAGC 
CCACTGTTTG GCATTAGCCG TCAGGACCTT GAGACAGACG ACTTTGAGAT TGTGGTCATC 
CTGGAGGGCA TGGTAGAGGC CACAGCCATG ACCACACAGG CTCGCAGTTC CTACCTGGCT 
AACGAGATCC TGTGGGGCCA CCGCTTTGAG CCAGTGCTCT TCGAAGAGAA GAACCAGTAC 
AAGATTGACT ATTCACACTT CCACAAGACC TACGAGGTGC CATCTACACC CCGCTGCAGC 
GCCAAGGACC TGGTGGAGAA CAAGTTCCTC CTGCCCAGCG CCAACTCTTT CTGCTATGAG 
AACGAGCTGG CCTTCCTGAG CAGAGATGAG GAGGACGAGG TGGCTACCGA CCGGGATGGC 
CGCAGCCCTC AGCCCGAGCA TGACTTTGAC AGACTGCAGG CCAGCAGCGC TGCCCTTGAA 
CGGCCCTACA GACGGGAGTC GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101466.1
CDS	3948..5537
Translation	MTRWTSLKASRPHKPGSWDLAASPRRLSTPTLATFPWKAQEPGAEMSSQNAPRVPQPRQPSASWPRQKQHPPLQPQASAKQAHEVPPPGVSLGAGQGPPDPGMTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVLQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEVATDRDGRSPQPEHDFDRLQASSAALERPYRRESEI

Target ORF information:

RefSeq Version	XM_030245606.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 12 (Kcnj12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245606.1

ORF Insert Sequence:

ATGACCCGCT GGACGTCCCT CAAGGCCTCT AGGCCACACA AGCCTGGCTC CTGGGATCTG 
GCAGCTTCCC CCCGGCGTCT GAGTACCCCA ACCTTGGCCA CCTTCCCCTG GAAGGCTCAG 
GAACCCGGGG CTGAGATGTC TTCTCAGAAT GCTCCCCGGG TTCCCCAGCC CCGTCAACCC 
TCTGCCTCCT GGCCTAGACA GAAACAGCAT CCACCATTAC AGCCCCAAGC CTCAGCTAAA 
CAAGCCCATG AAGTGCCCCC GCCTGGAGTC AGCCTGGGGG CAGGCCAGGG TCCACCAGAC 
CCCGGGATGA CCGCAGCCAG TCGGGCCAAC CCCTACAGCA TCGTATCATC AGAGGAGGAC 
GGGCTGCACC TGGTTACCAT GTCAGGCGCC AACGGTTTTG GCAATGGCAA GGTGCATACA 
CGGCGCCGGT GCCGCAACCG CTTCGTCAAG AAGAACGGTC AGTGCAACAT TGAATTCGCC 
AACATGGACG AGAAGTCACA ACGCTACCTG GCTGACATGT TTACCACGTG TGTGGACATC 
CGCTGGCGCT ACATGCTGCT CATCTTCTCT CTGGCCTTTC TTGCCTCCTG GTTGTTGTTT 
GGCATCATCT TCTGGGTCAT TGCTGTCGCC CACGGGGACC TGGAGCCAGC CGAGGGCCGT 
GGCCGTACAC CCTGTGTGCT GCAGGTCCAC GGCTTCATGG CAGCCTTTCT CTTCTCCATT 
GAGACACAGA CCACCATTGG CTACGGGCTA CGCTGTGTGA CTGAAGAGTG CCCGGTGGCT 
GTCTTCATGG TGGTGGCGCA GTCCATTGTG GGCTGCATCA TTGACTCCTT CATGATTGGT 
GCCATCATGG CCAAGATGGC ACGGCCCAAG AAGCGCGCAC AGACTCTGCT TTTCAGCCAT 
AATGCCGTGG TGGCTCTGCG TGACGGCAAG CTCTGCCTCA TGTGGCGCGT GGGCAACCTG 
CGTAAGAGTC ACATCGTGGA GGCCCATGTG CGGGCCCAGC TCATCAAGCC CAGGGTCACA 
GAGGAGGGTG AGTACATCCC ACTGGACCAG ATTGACATCG ATGTCGGCTT TGACAAGGGC 
CTAGACCGTA TCTTCCTGGT ATCACCCATC ACCATCTTGC ACGAGATTGA TGAGGCCAGC 
CCACTGTTTG GCATTAGCCG TCAGGACCTT GAGACAGACG ACTTTGAGAT TGTGGTCATC 
CTGGAGGGCA TGGTAGAGGC CACAGCCATG ACCACACAGG CTCGCAGTTC CTACCTGGCT 
AACGAGATCC TGTGGGGCCA CCGCTTTGAG CCAGTGCTCT TCGAAGAGAA GAACCAGTAC 
AAGATTGACT ATTCACACTT CCACAAGACC TACGAGGTGC CATCTACACC CCGCTGCAGC 
GCCAAGGACC TGGTGGAGAA CAAGTTCCTC CTGCCCAGCG CCAACTCTTT CTGCTATGAG 
AACGAGCTGG CCTTCCTGAG CAGAGATGAG GAGGACGAGG TGGCTACCGA CCGGGATGGC 
CGCAGCCCTC AGCCCGAGCA TGACTTTGAC AGACTGCAGG CCAGCAGCGC TGCCCTTGAA 
CGGCCCTACA GACGGGAGTC GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

PITX2 insufficiency leads to atrial electrical and structural remodeling linked to arrhythmogenesis.
Circulation. Cardiovascular genetics4(3)269-79(2011 Jun)
Chinchilla A,Daimi H,Lozano-Velasco E,Dominguez JN,Caballero R,Delp?n E,Tamargo J,Cinca J,Hove-Madsen L,Aranega AE,Franco D

Functional characterization of inward rectifier potassium ion channel in murine fetal ventricular cardiomyocytes.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology26(3)413-20(2010)
Liu A,Tang M,Xi J,Gao L,Zheng Y,Luo H,Hu X,Zhao F,Reppel M,Hescheler J,Liang H

Low-affinity spermine block mediating outward currents through Kir2.1 and Kir2.2 inward rectifier potassium channels.
The Journal of physiology583(Pt 3)891-908(2007 Sep)
Ishihara K,Yan DH

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Kcnj12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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