Gzmf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gzmf
Entrez Gene ID	14943
Full Name	granzyme F
Synonyms	CCP4,Ctla-7,Ctla7,MCSP-3
General protein information	Preferred Names granzyme F Names granzyme F AKA granzyme G C134 CTL serine protease 3 MCSP3 cytotoxic cell protease 4 cytotoxic serine protease 3 granzyme G
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C3\|14 28.19 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010374.3	NP_034504.1	granzyme F precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GZMH	NP_219491.1
Pan troglodytes (chimpanzee)	GZMH	XP_522811.2
Mus musculus (house mouse)	Gzmd	NP_034502.2
Mus musculus (house mouse)	Gzme	NP_034503.2
Mus musculus (house mouse)	Gzmg	NP_034505.1
Rattus norvegicus (Norway rat)	Gzmf	NP_703196.1
Mus musculus (house mouse)	Gzmf	NP_034504.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of the enzymatic and functional properties of three cytosolic carboxypeptidase family members.
Wu HY, Rong Y, Correia K, Min J, Morgan JI
The Journal of biological chemistry290(2)1222-32(2015 Jan)

Granzyme F induces a novel death pathway characterized by Bid-independent cytochrome c release without caspase activation.
Shi L, Wu L, Wang S, Fan Z
Cell death and differentiation16(12)1694-706(2009 Dec)

Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice.
Szabo R, Hobson JP, Christoph K, Kosa P, List K, Bugge TH
Development (Cambridge, England)136(15)2653-63(2009 Aug)

Csf2 null mutation alters placental gene expression and trophoblast glycogen cell and giant cell abundance in mice.
Sferruzzi-Perri AN, Macpherson AM, Roberts CT, Robertson SA
Biology of reproduction81(1)207-21(2009 Jul)

Expansion of the mast cell chymase locus over the past 200 million years of mammalian evolution.
Gallwitz M, Reimer JM, Hellman L
Immunogenetics58(8)655-69(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nna1 and CCP4 remove the C-terminal glutamate from substrates with two or more glutamates, whereas CCP6 requires four or more glutamates. CCP4 behaves as a promiscuous glutamase with little preference for chain length or neighboring amino acid composition
Title: Comparison of the enzymatic and functional properties of three cytosolic carboxypeptidase family members.

showed that GzmF impairs mitochondrial electron transport to abolish ATP generation; ATP decline may contribute to a failure of apoptosome formation, leading to caspase inactivation.
Title: Granzyme F induces a novel death pathway characterized by Bid-independent cytochrome c release without caspase activation.

The following Gzmf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gzmf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06068	NM_010374.3 Latest version!	Mus musculus granzyme F (Gzmf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gzmf ( NM_010374.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034504.1 Mus musculus granzyme F (Gzmf), mRNA.

Price & Availability↑

CloneID	OMu06068
Clone ID Related Accession (Same CDS sequence)	NM_010374.3
Accession Version	NM_010374.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	granzyme F precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC100311.1. On Oct 21, 2006 this sequence version replaced NM_010374.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100311.1, J03257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGCCACCAA TCCTGATTCT CCTGACCCTT CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGGG GCCATGAGGT CAAGCCCCAC TCCCGCCCTT ACATGGCACG TGTGAGGTTT 
GTGAAAGATA ATGGAAAAAG ACATTCCTGT GGAGGCTTCC TGGTTCAAGA CTACTTTGTG 
CTGACGGCTG CTCACTGCAC TGGAAGCTCA ATGAGAGTCA TACTGGGGGC CCACAACATC 
AGGGCTAAGG AAGAGACACA GCAGATCATC CCTGTGGCAA AAGCCATTCC CCACCCAGCT 
TATGATGATA AGGACAACAC CAGTGACATC ATGCTATTAA AGCTGGAGAG TAAGGCCAAG 
AGAACTAAAG CTGTGAGGCC CCTCAAGTTG CCCAGACCCA ATGCCCGGGT GAAGCCAGGG 
CATGTTTGCA GTGTGGCTGG CTGGGGGAGA ACATCCATCA ATGCAACACA AAGATCTTCC 
TGCCTACGAG AGGCTCAACT GATCATCCAG AAGGATAAGG AATGCAAAAA ATACTTCTAT 
AAGTATTTCA AGACCATGCA GATTTGTGCT GGAGACCCAA AGAAAATACA GTCTACTTAC 
AGTGGTGACT CCGGGGGACC CCTCGTGTGT AACAACAAAG CTTATGGAGT TTTAACCTAT 
GGGCTAAACA GGACAATCGG TCCAGGAGTC TTCACTAAGG TTGTGCACTA CCTGCCGTGG 
ATAAGCAGGA ACATGAAGCT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034504.1
CDS	51..797
Translation	MPPILILLTLLLPLRAGAEEIIGGHEVKPHSRPYMARVRFVKDNGKRHSCGGFLVQDYFVLTAAHCTGSSMRVILGAHNIRAKEETQQIIPVAKAIPHPAYDDKDNTSDIMLLKLESKAKRTKAVRPLKLPRPNARVKPGHVCSVAGWGRTSINATQRSSCLREAQLIIQKDKECKKYFYKYFKTMQICAGDPKKIQSTYSGDSGGPLVCNNKAYGVLTYGLNRTIGPGVFTKVVHYLPWISRNMKLL

Target ORF information:

RefSeq Version	NM_010374.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus granzyme F (Gzmf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010374.3

ORF Insert Sequence:

ATGCCACCAA TCCTGATTCT CCTGACCCTT CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGGG GCCATGAGGT CAAGCCCCAC TCCCGCCCTT ACATGGCACG TGTGAGGTTT 
GTGAAAGATA ATGGAAAAAG ACATTCCTGT GGAGGCTTCC TGGTTCAAGA CTACTTTGTG 
CTGACGGCTG CTCACTGCAC TGGAAGCTCA ATGAGAGTCA TACTGGGGGC CCACAACATC 
AGGGCTAAGG AAGAGACACA GCAGATCATC CCTGTGGCAA AAGCCATTCC CCACCCAGCT 
TATGATGATA AGGACAACAC CAGTGACATC ATGCTATTAA AGCTGGAGAG TAAGGCCAAG 
AGAACTAAAG CTGTGAGGCC CCTCAAGTTG CCCAGACCCA ATGCCCGGGT GAAGCCAGGG 
CATGTTTGCA GTGTGGCTGG CTGGGGGAGA ACATCCATCA ATGCAACACA AAGATCTTCC 
TGCCTACGAG AGGCTCAACT GATCATCCAG AAGGATAAGG AATGCAAAAA ATACTTCTAT 
AAGTATTTCA AGACCATGCA GATTTGTGCT GGAGACCCAA AGAAAATACA GTCTACTTAC 
AGTGGTGACT CCGGGGGACC CCTCGTGTGT AACAACAAAG CTTATGGAGT TTTAACCTAT 
GGGCTAAACA GGACAATCGG TCCAGGAGTC TTCACTAAGG TTGTGCACTA CCTGCCGTGG 
ATAAGCAGGA ACATGAAGCT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of the enzymatic and functional properties of three cytosolic carboxypeptidase family members.
The Journal of biological chemistry290(2)1222-32(2015 Jan)
Wu HY,Rong Y,Correia K,Min J,Morgan JI

Granzyme F induces a novel death pathway characterized by Bid-independent cytochrome c release without caspase activation.
Cell death and differentiation16(12)1694-706(2009 Dec)
Shi L,Wu L,Wang S,Fan Z

Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice.
Development (Cambridge, England)136(15)2653-63(2009 Aug)
Szabo R,Hobson JP,Christoph K,Kosa P,List K,Bugge TH

Csf2 null mutation alters placental gene expression and trophoblast glycogen cell and giant cell abundance in mice.
Biology of reproduction81(1)207-21(2009 Jul)
Sferruzzi-Perri AN,Macpherson AM,Roberts CT,Robertson SA

Expansion of the mast cell chymase locus over the past 200 million years of mammalian evolution.
Immunogenetics58(8)655-69(2006 Aug)
Gallwitz M,Reimer JM,Hellman L