Gzmd cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gzmd
Entrez Gene ID	14941
Full Name	granzyme D
Synonyms	CCP5,Ctla-5,Ctla5
General protein information	Preferred Names granzyme D Names granzyme D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C3\|14 28.19 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010372.2	NP_034502.2	granzyme D precursor
XM_006518569.4	XP_006518632.2	granzyme D isoform X1
XM_006518569.3	XP_006518632.2	granzyme D isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GZMH	NP_219491.1
Pan troglodytes (chimpanzee)	GZMH	XP_522811.2
Mus musculus (house mouse)	Gzmd	NP_034502.2
Mus musculus (house mouse)	Gzme	NP_034503.2
Mus musculus (house mouse)	Gzmg	NP_034505.1
Rattus norvegicus (Norway rat)	Gzmf	NP_703196.1
Mus musculus (house mouse)	Gzmf	NP_034504.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytosolic carboxypeptidase 5 removes ?- and ?-linked glutamates from tubulin.
Berezniuk I, Lyons PJ, Sironi JJ, Xiao H, Setou M, Angeletti RH, Ikegami K, Fricker LD
The Journal of biological chemistry288(42)30445-53(2013 Oct)

Granzyme D is a novel murine mast cell protease that is highly induced by multiple pathways of mast cell activation.
R?nnberg E, Calounova G, Guss B, Lundequist A, Pejler G
Infection and immunity81(6)2085-94(2013 Jun)

Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs).
Kimura Y, Kurabe N, Ikegami K, Tsutsumi K, Konishi Y, Kaplan OI, Kunitomo H, Iino Y, Blacque OE, Setou M
The Journal of biological chemistry285(30)22936-41(2010 Jul)

Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice.
Szabo R, Hobson JP, Christoph K, Kosa P, List K, Bugge TH
Development (Cambridge, England)136(15)2653-63(2009 Aug)

Csf2 null mutation alters placental gene expression and trophoblast glycogen cell and giant cell abundance in mice.
Sferruzzi-Perri AN, Macpherson AM, Roberts CT, Robertson SA
Biology of reproduction81(1)207-21(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings identify granzyme D as a novel murine mast cell protease and implicate granzyme D in settings where mast cells are activated, such as bacterial infection and allergy.
Title: Granzyme D is a novel murine mast cell protease that is highly induced by multiple pathways of mast cell activation.

The following Gzmd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gzmd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18227	NM_010372.2 Latest version!	Mus musculus granzyme D (Gzmd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu67897	XM_006518569.4 Latest version!	Mus musculus granzyme D (Gzmd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu67897	XM_006518569.3	Mus musculus granzyme D (Gzmd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gzmd ( NM_010372.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034502.2 Mus musculus granzyme D (Gzmd), mRNA.

Price & Availability↑

CloneID	OMu18227
Clone ID Related Accession (Same CDS sequence)	NM_010372.2
Accession Version	NM_010372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	granzyme D precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK131671.1. On Jul 25, 2007 this sequence version replaced NM_010372.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131671.1, J03255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGCCACCAA TCCTGATTCT CCTGACCCTA CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGCG GCCATGTGGT GAAGCCACAC TCCCGCCCCT ACATGGCATT CGTTATGTCT 
GTGGATATTA AGGGGAACAG GATATACTGT GGAGGCTTCC TGATTCAAGA TGACTTTGTG 
CTGACGGCTG CTCACTGCAA AAACAGCTCT GTCCAAAGCT CAATGACAGT CACACTGGGG 
GCCCACAACA TCACGGCTAA GGAGGAGACA CAGCAGATCA TCCCTGTGGC AAAAGACATT 
CCCCATCCAG ATTATAATGC TACTATCTTC TACAGTGACA TCATGCTGTT AAAGCTGGAG 
AGTAAGGCCA AGAGAACTAA AGCTGTGAGA CCCCTCAAGT TGCCCAGATC CAATGCCCGG 
GTGAAGCCAG GGGATGTGTG CAGTGTGGCT GGCTGGGGGT CAAGGTCCAT CAATGACACT 
AAAGCATCTG CCCGCCTGCG AGAGGTTCAA CTGGTCATCC AGGAGGACGA GGAATGCAAA 
AAACGTTTCC GATACTACAC TGAGACCACA GAGATTTGTG CTGGAGACTT GAAGAAAATA 
AAGACTCCTT TCAAGGGTGA CTCGGGGGGA CCCCTTGTGT GTGACAACCA AGCATATGGA 
CTTTTCGCCT ATGCAAAAAA CGGAACAATC TCTTCAGGAA TCTTCACTAA GGTTGTGCAC 
TTCCTGCCGT GGATAAGCTG GAACATGAAG TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034502.2
CDS	72..830
Translation	MPPILILLTLLLPLRAGAEEIIGGHVVKPHSRPYMAFVMSVDIKGNRIYCGGFLIQDDFVLTAAHCKNSSVQSSMTVTLGAHNITAKEETQQIIPVAKDIPHPDYNATIFYSDIMLLKLESKAKRTKAVRPLKLPRSNARVKPGDVCSVAGWGSRSINDTKASARLREVQLVIQEDEECKKRFRYYTETTEICAGDLKKIKTPFKGDSGGPLVCDNQAYGLFAYAKNGTISSGIFTKVVHFLPWISWNMKLL

Target ORF information:

RefSeq Version	NM_010372.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus granzyme D (Gzmd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010372.2

ORF Insert Sequence:

ATGCCACCAA TCCTGATTCT CCTGACCCTA CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGCG GCCATGTGGT GAAGCCACAC TCCCGCCCCT ACATGGCATT CGTTATGTCT 
GTGGATATTA AGGGGAACAG GATATACTGT GGAGGCTTCC TGATTCAAGA TGACTTTGTG 
CTGACGGCTG CTCACTGCAA AAACAGCTCT GTCCAAAGCT CAATGACAGT CACACTGGGG 
GCCCACAACA TCACGGCTAA GGAGGAGACA CAGCAGATCA TCCCTGTGGC AAAAGACATT 
CCCCATCCAG ATTATAATGC TACTATCTTC TACAGTGACA TCATGCTGTT AAAGCTGGAG 
AGTAAGGCCA AGAGAACTAA AGCTGTGAGA CCCCTCAAGT TGCCCAGATC CAATGCCCGG 
GTGAAGCCAG GGGATGTGTG CAGTGTGGCT GGCTGGGGGT CAAGGTCCAT CAATGACACT 
AAAGCATCTG CCCGCCTGCG AGAGGTTCAA CTGGTCATCC AGGAGGACGA GGAATGCAAA 
AAACGTTTCC GATACTACAC TGAGACCACA GAGATTTGTG CTGGAGACTT GAAGAAAATA 
AAGACTCCTT TCAAGGGTGA CTCGGGGGGA CCCCTTGTGT GTGACAACCA AGCATATGGA 
CTTTTCGCCT ATGCAAAAAA CGGAACAATC TCTTCAGGAA TCTTCACTAA GGTTGTGCAC 
TTCCTGCCGT GGATAAGCTG GAACATGAAG TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gzmd ( XM_006518569.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518632.2 Mus musculus granzyme D (Gzmd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67897
Clone ID Related Accession (Same CDS sequence)	XM_006518569.4 , XM_006518569.3
Accession Version	XM_006518569.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	granzyme D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAA TCCTGATTCT CCTGACCCTA CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGCG GCCATGTGGT GAAGCCACAC TCCCGCCCCT ACATGGCATT CGTTATGTCT 
GTGGATATTA AGGGGAACAG GATATACTGT GGAGGCTTCC TGATTCAAGA TGACTTTGTG 
CTGACGGCTG CTCACTGCAA AAACAGCTCA ATGACAGTCA CACTGGGGGC CCACAACATC 
ACGGCTAAGG AGGAGACACA GCAGATCATC CCTGTGGCAA AAGACATTCC CCATCCAGAT 
TATAATGCTA CTATCTTCTA CAGTGACATC ATGCTGTTAA AGCTGGAGAG TAAGGCCAAG 
AGAACTAAAG CTGTGAGACC CCTCAAGTTG CCCAGATCCA ATGCCCGGGT GAAGCCAGGG 
GATGTGTGCA GTGTGGCTGG CTGGGGGTCA AGGTCCATCA ATGACACTAA AGCATCTGCC 
CGCCTGCGAG AGGTTCAACT GGTCATCCAG GAGGACGAGG AATGCAAAAA ACGTTTCCGA 
TACTACACTG AGACCACAGA GATTTGTGCT GGAGACTTGA AGAAAATAAA GACTCCTTTC 
AAGGGTGACT CGGGGGGACC CCTTGTGTGT GACAACCAAG CATATGGACT TTTCGCCTAT 
GCAAAAAACG GAACAATCTC TTCAGGAATC TTCACTAAGG TTGTGCACTT CCTGCCGTGG 
ATAAGCTGGA ACATGAAGTT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518632.2
CDS	86..832
Translation	MPPILILLTLLLPLRAGAEEIIGGHVVKPHSRPYMAFVMSVDIKGNRIYCGGFLIQDDFVLTAAHCKNSSMTVTLGAHNITAKEETQQIIPVAKDIPHPDYNATIFYSDIMLLKLESKAKRTKAVRPLKLPRSNARVKPGDVCSVAGWGSRSINDTKASARLREVQLVIQEDEECKKRFRYYTETTEICAGDLKKIKTPFKGDSGGPLVCDNQAYGLFAYAKNGTISSGIFTKVVHFLPWISWNMKLL

Target ORF information:

RefSeq Version	XM_006518569.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus granzyme D (Gzmd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518569.3

ORF Insert Sequence:

ATGCCACCAA TCCTGATTCT CCTGACCCTA CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGCG GCCATGTGGT GAAGCCACAC TCCCGCCCCT ACATGGCATT CGTTATGTCT 
GTGGATATTA AGGGGAACAG GATATACTGT GGAGGCTTCC TGATTCAAGA TGACTTTGTG 
CTGACGGCTG CTCACTGCAA AAACAGCTCA ATGACAGTCA CACTGGGGGC CCACAACATC 
ACGGCTAAGG AGGAGACACA GCAGATCATC CCTGTGGCAA AAGACATTCC CCATCCAGAT 
TATAATGCTA CTATCTTCTA CAGTGACATC ATGCTGTTAA AGCTGGAGAG TAAGGCCAAG 
AGAACTAAAG CTGTGAGACC CCTCAAGTTG CCCAGATCCA ATGCCCGGGT GAAGCCAGGG 
GATGTGTGCA GTGTGGCTGG CTGGGGGTCA AGGTCCATCA ATGACACTAA AGCATCTGCC 
CGCCTGCGAG AGGTTCAACT GGTCATCCAG GAGGACGAGG AATGCAAAAA ACGTTTCCGA 
TACTACACTG AGACCACAGA GATTTGTGCT GGAGACTTGA AGAAAATAAA GACTCCTTTC 
AAGGGTGACT CGGGGGGACC CCTTGTGTGT GACAACCAAG CATATGGACT TTTCGCCTAT 
GCAAAAAACG GAACAATCTC TTCAGGAATC TTCACTAAGG TTGTGCACTT CCTGCCGTGG 
ATAAGCTGGA ACATGAAGTT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gzmd ( XM_006518569.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518632.2 Mus musculus granzyme D (Gzmd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67897
Clone ID Related Accession (Same CDS sequence)	XM_006518569.4 , XM_006518569.3
Accession Version	XM_006518569.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	granzyme D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518569.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAA TCCTGATTCT CCTGACCCTA CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGCG GCCATGTGGT GAAGCCACAC TCCCGCCCCT ACATGGCATT CGTTATGTCT 
GTGGATATTA AGGGGAACAG GATATACTGT GGAGGCTTCC TGATTCAAGA TGACTTTGTG 
CTGACGGCTG CTCACTGCAA AAACAGCTCA ATGACAGTCA CACTGGGGGC CCACAACATC 
ACGGCTAAGG AGGAGACACA GCAGATCATC CCTGTGGCAA AAGACATTCC CCATCCAGAT 
TATAATGCTA CTATCTTCTA CAGTGACATC ATGCTGTTAA AGCTGGAGAG TAAGGCCAAG 
AGAACTAAAG CTGTGAGACC CCTCAAGTTG CCCAGATCCA ATGCCCGGGT GAAGCCAGGG 
GATGTGTGCA GTGTGGCTGG CTGGGGGTCA AGGTCCATCA ATGACACTAA AGCATCTGCC 
CGCCTGCGAG AGGTTCAACT GGTCATCCAG GAGGACGAGG AATGCAAAAA ACGTTTCCGA 
TACTACACTG AGACCACAGA GATTTGTGCT GGAGACTTGA AGAAAATAAA GACTCCTTTC 
AAGGGTGACT CGGGGGGACC CCTTGTGTGT GACAACCAAG CATATGGACT TTTCGCCTAT 
GCAAAAAACG GAACAATCTC TTCAGGAATC TTCACTAAGG TTGTGCACTT CCTGCCGTGG 
ATAAGCTGGA ACATGAAGTT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518632.2
CDS	82..828
Translation	MPPILILLTLLLPLRAGAEEIIGGHVVKPHSRPYMAFVMSVDIKGNRIYCGGFLIQDDFVLTAAHCKNSSMTVTLGAHNITAKEETQQIIPVAKDIPHPDYNATIFYSDIMLLKLESKAKRTKAVRPLKLPRSNARVKPGDVCSVAGWGSRSINDTKASARLREVQLVIQEDEECKKRFRYYTETTEICAGDLKKIKTPFKGDSGGPLVCDNQAYGLFAYAKNGTISSGIFTKVVHFLPWISWNMKLL

Target ORF information:

RefSeq Version	XM_006518569.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus granzyme D (Gzmd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518569.4

ORF Insert Sequence:

ATGCCACCAA TCCTGATTCT CCTGACCCTA CTTCTGCCTC TCAGAGCTGG AGCAGAGGAG 
ATCATCGGCG GCCATGTGGT GAAGCCACAC TCCCGCCCCT ACATGGCATT CGTTATGTCT 
GTGGATATTA AGGGGAACAG GATATACTGT GGAGGCTTCC TGATTCAAGA TGACTTTGTG 
CTGACGGCTG CTCACTGCAA AAACAGCTCA ATGACAGTCA CACTGGGGGC CCACAACATC 
ACGGCTAAGG AGGAGACACA GCAGATCATC CCTGTGGCAA AAGACATTCC CCATCCAGAT 
TATAATGCTA CTATCTTCTA CAGTGACATC ATGCTGTTAA AGCTGGAGAG TAAGGCCAAG 
AGAACTAAAG CTGTGAGACC CCTCAAGTTG CCCAGATCCA ATGCCCGGGT GAAGCCAGGG 
GATGTGTGCA GTGTGGCTGG CTGGGGGTCA AGGTCCATCA ATGACACTAA AGCATCTGCC 
CGCCTGCGAG AGGTTCAACT GGTCATCCAG GAGGACGAGG AATGCAAAAA ACGTTTCCGA 
TACTACACTG AGACCACAGA GATTTGTGCT GGAGACTTGA AGAAAATAAA GACTCCTTTC 
AAGGGTGACT CGGGGGGACC CCTTGTGTGT GACAACCAAG CATATGGACT TTTCGCCTAT 
GCAAAAAACG GAACAATCTC TTCAGGAATC TTCACTAAGG TTGTGCACTT CCTGCCGTGG 
ATAAGCTGGA ACATGAAGTT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytosolic carboxypeptidase 5 removes ?- and ?-linked glutamates from tubulin.
The Journal of biological chemistry288(42)30445-53(2013 Oct)
Berezniuk I,Lyons PJ,Sironi JJ,Xiao H,Setou M,Angeletti RH,Ikegami K,Fricker LD

Granzyme D is a novel murine mast cell protease that is highly induced by multiple pathways of mast cell activation.
Infection and immunity81(6)2085-94(2013 Jun)
R?nnberg E,Calounova G,Guss B,Lundequist A,Pejler G

Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs).
The Journal of biological chemistry285(30)22936-41(2010 Jul)
Kimura Y,Kurabe N,Ikegami K,Tsutsumi K,Konishi Y,Kaplan OI,Kunitomo H,Iino Y,Blacque OE,Setou M

Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice.
Development (Cambridge, England)136(15)2653-63(2009 Aug)
Szabo R,Hobson JP,Christoph K,Kosa P,List K,Bugge TH

Csf2 null mutation alters placental gene expression and trophoblast glycogen cell and giant cell abundance in mice.
Biology of reproduction81(1)207-21(2009 Jul)
Sferruzzi-Perri AN,Macpherson AM,Roberts CT,Robertson SA