Grpr cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grpr
Entrez Gene ID	14829
Full Name	gastrin releasing peptide receptor
Synonyms	GRP-R
General protein information	Preferred Names gastrin releasing peptide receptor Names gastrin-releasing peptide receptor GRP-preferring bombesin receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F4\|X 75.63 cM
Summary	This gene encodes a multipass membrane protein that functions as a receptor for gastrin releasing peptide. Signalling through this receptor is important in a variety of physiological processes, including responses to itching, satiety, and fear. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008177.3	NP_032203.1	gastrin-releasing peptide receptor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRPR	NP_005305.1
	GRPR	XP_001102545.1
Bos taurus (cattle)	GRPR	NP_001179668.1
Pan troglodytes (chimpanzee)	GRPR	XP_520952.2
Xenopus tropicalis (tropical clawed frog)	grpr	XP_002938295.1
Mus musculus (house mouse)	Grpr	NP_032203.1
Gallus gallus (chicken)	GRPR	NP_989738.1
Danio rerio (zebrafish)	grpr	XP_695673.2
Canis lupus familiaris (dog)	GRPR	XP_548874.2
Rattus norvegicus (Norway rat)	Grpr	NP_036838.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular organization of the mouse gastrin-releasing peptide receptor gene and its promoter.
Weber HC, Jensen RT, Battey JF
Gene244(1-2)137-49(2000 Feb)

Molecular cloning and characterization of receptors for the mammalian bombesin-like peptides.
Giladi E, Nagalla SR, Spindel ER
Journal of molecular neuroscience : MN4(1)41-54(1993)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ablating GRP receptor-expressing neurons eliminated basal and hypoxia-induced sighing, but left breathing otherwise intact initially
Title: The peptidergic control circuit for sighing.

Together, we define the respective function of NMBR and GRPR in itch transmission, and reveal an unexpected relationship not only between the two receptors but also between the two populations of interneurons in itch signaling.
Title: Cross-inhibition of NMBR and GRPR signaling maintains normal histaminergic itch transmission.

BNP-NPRA signaling is involved in both itch and pain and does not function upstream of the GRP-GRPR dedicated neuronal pathway.
Title: B-type natriuretic peptide is neither itch-specific nor functions upstream of the GRP-GRPR signaling pathway.

The present results suggest that BB2 receptor-expressing spinal neurons transmit herpes-associated itch by BB2 receptor-independent signaling.
Title: BB2 bombesin receptor-expressing spinal neurons transmit herpes-associated itch by BB2 receptor-independent signaling.

spinal GRPr and NMBr independently drive itch neurotransmission in mice.
Title: Physiological function of gastrin-releasing peptide and neuromedin B receptors in regulating itch scratching behavior in the spinal cord of mice.

The following Grpr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grpr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14220	NM_008177.3 Latest version!	Mus musculus gastrin releasing peptide receptor (Grpr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grpr ( NM_008177.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032203.1 Mus musculus gastrin releasing peptide receptor (Grpr), mRNA.

Price & Availability↑

CloneID	OMu14220
Clone ID Related Accession (Same CDS sequence)	NM_008177.3
Accession Version	NM_008177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	gastrin-releasing peptide receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL670292.9 and AK033473.1. On May 8, 2015 this sequence version replaced NM_008177.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK033473.1, BC113145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGGCTCCAA ATAATTGTTC CCACCTGAAC TTGGACGTGG ACCCTTTCCT GTCCTGCAAC 
GACACCTTCA ATCAAAGTCT GAGTCCCCCC AAGATGGACA ACTGGTTTCA CCCGGGCTTC 
ATCTATGTCA TCCCTGCAGT TTATGGGCTT ATCATCGTGA TAGGTCTTAT TGGCAACATC 
ACGCTCATCA AGATCTTCTG CACGGTCAAG TCCATGCGAA ACGTGCCAAA CCTGTTCATC 
TCTAGCCTGG CTTTGGGAGA CCTGCTGCTG CTGGTGACAT GCGCCCCTGT GGATGCCAGC 
AAGTACCTGG CTGACAGGTG GCTATTTGGC AGAATTGGCT GCAAACTGAT CCCCTTTATA 
CAACTTACTT CAGTGGGGGT GTCTGTCTTC ACACTTACGG CACTGTCAGC TGACAGGTAC 
AAAGCCATTG TACGGCCAAT GGATATCCAG GCATCCCATG CCCTGATGAA GATCTGTCTC 
AAAGCTGCTT TGATCTGGAT TGTCTCTATG TTGTTGGCCA TCCCAGAGGC TGTGTTTTCT 
GACCTCCACC CCTTCCATGT GAAAGATACC AACCAAACCT TCATTAGTTG TGCCCCCTAC 
CCACACTCCA ATGAGCTACA CCCTAAAATC CATTCCATGG CTTCCTTTCT GGTTTTCTAC 
GTTATCCCAC TGGCGATCAT CTCTGTCTAC TACTACTTCA TTGCCCGAAA TCTGATTCAG 
AGTGCCTACA ATCTTCCCGT GGAAGGCAAT ATACATGTCA AGAAGCAGAT CGAATCCCGG 
AAGCGGCTTG CCAAGACAGT ACTGGTGTTT GTGGGCCTCT TTGCCTTCTG CTGGCTCCCC 
AACCATGTCA TCTACCTGTA CCGTTCCTAC CACTACTCTG AAGTGGACAC CTCCATGCTC 
CACTTTGTCA CCAGCATCTG TGCCCGCCTC CTGGCCTTCA CCAACTCCTG TGTGAACCCC 
TTTGCTCTTT ATCTGCTGAG CAAGAGCTTC AGGAAGCAGT TCAACACTCA ACTTCTCTGC 
TGCCAGCCTG GCCTGATGAA CAGGTCCCAC AGCACAGGCA GAAGTACCAC CTGCATGACC 
TCCTTCAAGA GCACTAACCC CTCGGCTACC TTTAGCCTCA TCAACAGAAA TATCTGTCAT 
GAGGGGTATG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032203.1
CDS	440..1594
Translation	MAPNNCSHLNLDVDPFLSCNDTFNQSLSPPKMDNWFHPGFIYVIPAVYGLIIVIGLIGNITLIKIFCTVKSMRNVPNLFISSLALGDLLLLVTCAPVDASKYLADRWLFGRIGCKLIPFIQLTSVGVSVFTLTALSADRYKAIVRPMDIQASHALMKICLKAALIWIVSMLLAIPEAVFSDLHPFHVKDTNQTFISCAPYPHSNELHPKIHSMASFLVFYVIPLAIISVYYYFIARNLIQSAYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFCWLPNHVIYLYRSYHYSEVDTSMLHFVTSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQLLCCQPGLMNRSHSTGRSTTCMTSFKSTNPSATFSLINRNICHEGYV

Target ORF information:

RefSeq Version	NM_008177.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gastrin releasing peptide receptor (Grpr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008177.3

ORF Insert Sequence:

ATGGCTCCAA ATAATTGTTC CCACCTGAAC TTGGACGTGG ACCCTTTCCT GTCCTGCAAC 
GACACCTTCA ATCAAAGTCT GAGTCCCCCC AAGATGGACA ACTGGTTTCA CCCGGGCTTC 
ATCTATGTCA TCCCTGCAGT TTATGGGCTT ATCATCGTGA TAGGTCTTAT TGGCAACATC 
ACGCTCATCA AGATCTTCTG CACGGTCAAG TCCATGCGAA ACGTGCCAAA CCTGTTCATC 
TCTAGCCTGG CTTTGGGAGA CCTGCTGCTG CTGGTGACAT GCGCCCCTGT GGATGCCAGC 
AAGTACCTGG CTGACAGGTG GCTATTTGGC AGAATTGGCT GCAAACTGAT CCCCTTTATA 
CAACTTACTT CAGTGGGGGT GTCTGTCTTC ACACTTACGG CACTGTCAGC TGACAGGTAC 
AAAGCCATTG TACGGCCAAT GGATATCCAG GCATCCCATG CCCTGATGAA GATCTGTCTC 
AAAGCTGCTT TGATCTGGAT TGTCTCTATG TTGTTGGCCA TCCCAGAGGC TGTGTTTTCT 
GACCTCCACC CCTTCCATGT GAAAGATACC AACCAAACCT TCATTAGTTG TGCCCCCTAC 
CCACACTCCA ATGAGCTACA CCCTAAAATC CATTCCATGG CTTCCTTTCT GGTTTTCTAC 
GTTATCCCAC TGGCGATCAT CTCTGTCTAC TACTACTTCA TTGCCCGAAA TCTGATTCAG 
AGTGCCTACA ATCTTCCCGT GGAAGGCAAT ATACATGTCA AGAAGCAGAT CGAATCCCGG 
AAGCGGCTTG CCAAGACAGT ACTGGTGTTT GTGGGCCTCT TTGCCTTCTG CTGGCTCCCC 
AACCATGTCA TCTACCTGTA CCGTTCCTAC CACTACTCTG AAGTGGACAC CTCCATGCTC 
CACTTTGTCA CCAGCATCTG TGCCCGCCTC CTGGCCTTCA CCAACTCCTG TGTGAACCCC 
TTTGCTCTTT ATCTGCTGAG CAAGAGCTTC AGGAAGCAGT TCAACACTCA ACTTCTCTGC 
TGCCAGCCTG GCCTGATGAA CAGGTCCCAC AGCACAGGCA GAAGTACCAC CTGCATGACC 
TCCTTCAAGA GCACTAACCC CTCGGCTACC TTTAGCCTCA TCAACAGAAA TATCTGTCAT 
GAGGGGTATG TCTAG