Gnai2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnai2
Entrez Gene ID	14678
Full Name	guanine nucleotide binding protein (G protein), alpha inhibiting 2
Synonyms	C76432,Galphai2,Gia,Gnai-2
General protein information	Preferred Names guanine nucleotide binding protein (G protein), alpha inhibiting 2 Names guanine nucleotide-binding protein G(i) subunit alpha-2 adenylate cyclase-inhibiting G alpha protein guanine nucleotide binding protein, alpha inhibiting 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F1\|9 58.43 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008138.5	NP_032164.2	guanine nucleotide-binding protein G(i) subunit alpha-2
XM_006511637.3	XP_006511700.1	guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1
XM_006511637.2	XP_006511700.1	guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1
NM_008138.4	NP_032164.2	guanine nucleotide-binding protein G(i) subunit alpha-2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GNAI2	NP_990733.1
Canis lupus familiaris (dog)	GNAI2	NP_001003364.1
Mus musculus (house mouse)	Gnai2	NP_032164.2
Danio rerio (zebrafish)	gnai2b	NP_956136.1
Saccharomyces cerevisiae (baker's yeast)	GPA1	NP_011868.1
Xenopus tropicalis (tropical clawed frog)	gnai2	NP_989250.1
Homo sapiens (human)	GNAI2	NP_002061.1
Pan troglodytes (chimpanzee)	GNAI2	XP_001167942.1
	GNAI2	XP_001103122.1
Bos taurus (cattle)	GNAI2	NP_001094510.1
Magnaporthe oryzae (rice blast fungus)	MGG_00365	XP_003718715.1
Rattus norvegicus (Norway rat)	Gnai2	NP_112297.1
Kluyveromyces lactis	KLLA0F25916g	XP_456234.1
Eremothecium gossypii	AGOS_ADR153C	NP_984250.1
Neurospora crassa	NCU06493	XP_957133.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential effects of inhibitory G-protein isoforms on G-protein gated inwardly rectifying K
Nobles M, Montaigne D, Sebastian S, Birnbaumer L, Tinker A
American journal of physiology. Cell physiology(2018 Jan)

Loss of G?
Hwang IY, Harrison K, Park C, Kehrl JH
Scientific reports7(1)4156(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reveals how RGS proteins modulate Galphai2 signaling to facilitate thymocyte egress and T cell trafficking.
Title: Normal Thymocyte Egress, T Cell Trafficking, and CD4

In Galphai2-knockdown mice, they showed defects in social interaction, novelty recognition and active avoidance learning.
Title: Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability.

this study shows that Galphai2 controls arrest in response to chemokine stimulation of neutrophils
Title: G?i2 and G?i3 Differentially Regulate Arrest from Flow and Chemotaxis in Mouse Neutrophils.

Data show that inhibitory (Galphai2) and stimulatory (GalphasL) G-protein subunits produced minor atrophic and hypertrophic changes in muscle mass, and Galphai2 over-expression prevented AAV:beta2-adrenoceptor (beta2-AR) mediated hypertrophy.
Title: Using AAV vectors expressing the ?2-adrenoceptor or associated G? proteins to modulate skeletal muscle mass and muscle fibre size.

Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing.
Title: The GPSM2/LGN GoLoco motifs are essential for hearing.

The following Gnai2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnai2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15664	NM_008138.5 Latest version!	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu15664	XM_006511637.3 Latest version!	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu15664	XM_006511637.2	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu15664	NM_008138.4	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnai2 ( NM_008138.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032164.2 Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), mRNA.

Price & Availability↑

CloneID	OMu15664
Clone ID Related Accession (Same CDS sequence)	NM_008138.5 , XM_006511637.3 , XM_006511637.2 , NM_008138.4
Accession Version	NM_008138.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	guanine nucleotide-binding protein G(i) subunit alpha-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ134826.1, AK167388.1 and CF951009.1. On Jun 16, 2018 this sequence version replaced NM_008138.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159222.1, AK167388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032164.2
CDS	127..1194
Translation	MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRADDARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITQSSLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	NM_008138.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008138.5

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnai2 ( NM_008138.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032164.2 Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), mRNA.

Price & Availability↑

CloneID	OMu15664
Clone ID Related Accession (Same CDS sequence)	NM_008138.5 , XM_006511637.3 , XM_006511637.2 , NM_008138.4
Accession Version	NM_008138.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-09
Organism	Mus musculus(house mouse)
Product	guanine nucleotide-binding protein G(i) subunit alpha-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK167388.1. On Apr 6, 2007 this sequence version replaced NM_008138.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK167388.1, AK159222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032164.2
CDS	126..1193
Translation	MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRADDARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITQSSLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	NM_008138.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008138.4

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnai2 ( XM_006511637.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511700.1 Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15664
Clone ID Related Accession (Same CDS sequence)	NM_008138.5 , XM_006511637.3 , XM_006511637.2 , NM_008138.4
Accession Version	XM_006511637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511700.1
CDS	175..1242
Translation	MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRADDARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITQSSLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_006511637.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511637.3

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnai2 ( XM_006511637.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511700.1 Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15664
Clone ID Related Accession (Same CDS sequence)	NM_008138.5 , XM_006511637.3 , XM_006511637.2 , NM_008138.4
Accession Version	XM_006511637.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006511637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511700.1
CDS	175..1242
Translation	MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRADDARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITQSSLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_006511637.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 2 (Gnai2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511637.2

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA AATGATCGAC 
AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT TCTGTTAGGT 
GCTGGAGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA TGAAGATGGC 
TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC CATCCAGTCT 
ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA TCCCCAGCGT 
GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA AGGGATGCTT 
CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT GCAAGCCTGC 
TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT GAATGATCTG 
GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCAGG ATGTGCTGCG GACCCGTGTG 
AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT CAAGATGTTT 
GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACG 
GCCATCATCT TCTGTGTCGC CTTGAGCGCT TATGACTTGG TGCTGGCTGA GGATGAGGAG 
ATGAACCGCA TGCATGAGAG CATGAAGCTG TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACCT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAAGA AAAGATCACA 
CAGAGCTCCC TGACCATCTG TTTCCCTGAG TACACGGGGG CCAACAAGTA CGACGAGGCA 
GCCAGCTACA TCCAGAGCAA GTTTGAGGAT CTAAATAAGC GCAAAGACAC CAAGGAGATC 
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTTGTGTT CGATGCCGTC 
ACTGACGTCA TCATCAAGAA CAACCTGAAG GACTGTGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential effects of inhibitory G-protein isoforms on G-protein gated inwardly rectifying K
American journal of physiology. Cell physiology(2018 Jan)
Nobles M,Montaigne D,Sebastian S,Birnbaumer L,Tinker A

Loss of G?
Scientific reports7(1)4156(2017 Jun)
Hwang IY,Harrison K,Park C,Kehrl JH

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Gnai2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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