Ostm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ostm1
Entrez Gene ID	14628
Full Name	osteopetrosis associated transmembrane protein 1
Synonyms	1200002H13Rik,HSPC019,gl
General protein information	Preferred Names osteopetrosis associated transmembrane protein 1 Names osteopetrosis-associated transmembrane protein 1 chloride channel 7 beta subunit grey lethal osteopetrosis
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B2\|10 22.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_172416.3	NP_766004.1	osteopetrosis-associated transmembrane protein 1 precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ostm1	NP_001072582.1
Pan troglodytes (chimpanzee)	OSTM1	XP_001148469.1
	OSTM1	XP_001094902.1
Canis lupus familiaris (dog)	OSTM1	XP_854990.3
Gallus gallus (chicken)	OSTM1	NP_001026248.1
Homo sapiens (human)	OSTM1	NP_054747.2
Mus musculus (house mouse)	Ostm1	NP_766004.1
Rattus norvegicus (Norway rat)	Ostm1	NP_001025096.1
Bos taurus (cattle)	OSTM1	NP_001069244.1
Danio rerio (zebrafish)	ostm1	XP_002665788.2

Gene Ontology
Bibliography

Related articles in PubMed

Ostm1 bifunctional roles in osteoclast maturation: insights from a mouse model mimicking a human OSTM1 mutation.
Pata M, Vacher J
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research(2018 Jan)

Role of Ostm1 Cytosolic Complex with Kinesin 5B in Intracellular Dispersion and Trafficking.
Pandruvada SN, Beauregard J, Benjannet S, Pata M, Lazure C, Seidah NG, Vacher J
Molecular and cellular biology36(3)507-21(2015 Nov)

Secretion of a truncated osteopetrosis-associated transmembrane protein 1 (OSTM1) mutant inhibits osteoclastogenesis through down-regulation of the B lymphocyte-induced maturation protein 1 (BLIMP1)-nuclear factor of activated T cells c1 (NFATc1) axis.
Shin B, Yu J, Park ES, Choi S, Yu J, Hwang JM, Yun H, Chung YH, Hong KS, Choi JS, Takami M, Rho J
The Journal of biological chemistry289(52)35868-81(2014 Dec)

Transport activity and presence of ClC-7/Ostm1 complex account for different cellular functions.
Weinert S, Jabs S, Hohensee S, Chan WL, Kornak U, Jentsch TJ
EMBO reports15(7)784-91(2014 Jul)

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Brommage R, Liu J, Hansen GM, Kirkpatrick LL, Potter DG, Sands AT, Zambrowicz B, Powell DR, Vogel P
Bone research214034(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KIF5B is essential for Ostm1 intracellular dispersion.
Title: Role of Ostm1 Cytosolic Complex with Kinesin 5B in Intracellular Dispersion and Trafficking.

Truncated OSTM1 significantly inhibited the expression of osteoclast marker genes through the down-regulation of the BLIMP1-NFATc1 axis.
Title: Secretion of a truncated osteopetrosis-associated transmembrane protein 1 (OSTM1) mutant inhibits osteoclastogenesis through down-regulation of the B lymphocyte-induced maturation protein 1 (BLIMP1)-nuclear factor of activated T cells c1 (NFATc1) axis.

Our in vivo structure-function analysis of ClC-7 reveals that both protein-protein interactions and ion transport must be considered in the pathogenesis of ClC-7-related diseases.
Title: Transport activity and presence of ClC-7/Ostm1 complex account for different cellular functions.

Ostm1 has a primary and autonomous role in neuronal homeostasis
Title: Severe neurodegeneration with impaired autophagy mechanism triggered by ostm1 deficiency.

Ostm1 is a bona fide target of miR-140, which is significantly decreased during adipogenic differentiation.
Title: MicroRNA-140 promotes adipocyte lineage commitment of C3H10T1/2 pluripotent stem cells via targeting osteopetrosis-associated transmembrane protein 1.

The following Ostm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ostm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15662	NM_172416.3 Latest version!	Mus musculus osteopetrosis associated transmembrane protein 1 (Ostm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ostm1 ( NM_172416.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766004.1 Mus musculus osteopetrosis associated transmembrane protein 1 (Ostm1), mRNA.

Price & Availability↑

CloneID	OMu15662
Clone ID Related Accession (Same CDS sequence)	NM_172416.3
Accession Version	NM_172416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	osteopetrosis-associated transmembrane protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK044897.1, AF533890.1 and BB286500.1. On Mar 1, 2011 this sequence version replaced NM_172416.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK044897.1, AF533890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGGG ACGCGGAGCT GGCGCGCAGT AGCGGGTGGC CGTGGCGGTG GCTGCCGGCG 
CTGCTGCTGC TGCAGCTGCT GCGGTGGAGG TGCGCCCTGT GCGCGCTCCC CTTCACCAGC 
AGTCGGCACC CAGGCTTTGC GGACCTGCTG TCGGAGCAGC AGCTGTTGGA GGTGCAGGAC 
TTGACCCTGT CTTTGCTGCA GGGCGGAGGT CTAGGGCCGC TGTCACTGCT ACCTCCGGAC 
CTGCCGGATC TGGAGCCTGA GTGCCGGGAG CTGCTGATGG ACTTCGCCAA TAGCAGCGCC 
GAGCTGACCG CCTGTATGGT GCGCAGCGCT CGGCCCGTGC GCCTCTGCCA GACCTGCTAC 
CCGCTCTTCC AACAGGTCGC AATCAAGATG GACAACATCA GCCGAAACAT CGGGAATACC 
TCCGAGGGCC CGCGCTGTGG CGGAAGTCTC CTGACGGCAG ACAGAATGCA GATAGTTCTC 
ATGGTCTCTG AGTTTTTCAA CAGCACGTGG CAGGAGGCGA ACTGCGCAAA TTGCCTAACA 
AACAATGGTG AGGATTTGTC AAACAACACA GAGGACTTCC TCAGTCTGTT TAACAAGACT 
TTGGCCTGCT TTGAGCATAA CCTGCAGGGG CACACATACA GTCTCCTCCC ACCAAAAAAT 
TACTCCGAAG TGTGCAGAAA CTGTAAAGAG GCATATAAAA ACCTGAGCCT CCTGTACAGT 
CAAATGCAGA AACTGAACGG GCTTGAGAAC AAGGCTGAGC CTGAGACGCA CTTGTGCATC 
GATGTGGAGG ATGCAATGAA CATTACTCGG AAGCTTTGGA GTCGAACCTT CAACTGTTCG 
GTCACCTGCA GCGACACGGT GTCCGTGGTT GCTGTGTCTG TGTTCATTCT CTTCCTGCCT 
GTCGTCTTCT ACCTCAGTAG CTTCCTTCAC TCAGAGCAAA AGAAACGCAA ACTCATTCTA 
CCCAAACGTC TCAAGTCGAG CACCAGTTTT GCCAACATTC AAGAAAATGC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766004.1
CDS	69..1085
Translation	MARDAELARSSGWPWRWLPALLLLQLLRWRCALCALPFTSSRHPGFADLLSEQQLLEVQDLTLSLLQGGGLGPLSLLPPDLPDLEPECRELLMDFANSSAELTACMVRSARPVRLCQTCYPLFQQVAIKMDNISRNIGNTSEGPRCGGSLLTADRMQIVLMVSEFFNSTWQEANCANCLTNNGEDLSNNTEDFLSLFNKTLACFEHNLQGHTYSLLPPKNYSEVCRNCKEAYKNLSLLYSQMQKLNGLENKAEPETHLCIDVEDAMNITRKLWSRTFNCSVTCSDTVSVVAVSVFILFLPVVFYLSSFLHSEQKKRKLILPKRLKSSTSFANIQENAT

Target ORF information:

RefSeq Version	NM_172416.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus osteopetrosis associated transmembrane protein 1 (Ostm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172416.3

ORF Insert Sequence:

ATGGCTCGGG ACGCGGAGCT GGCGCGCAGT AGCGGGTGGC CGTGGCGGTG GCTGCCGGCG 
CTGCTGCTGC TGCAGCTGCT GCGGTGGAGG TGCGCCCTGT GCGCGCTCCC CTTCACCAGC 
AGTCGGCACC CAGGCTTTGC GGACCTGCTG TCGGAGCAGC AGCTGTTGGA GGTGCAGGAC 
TTGACCCTGT CTTTGCTGCA GGGCGGAGGT CTAGGGCCGC TGTCACTGCT ACCTCCGGAC 
CTGCCGGATC TGGAGCCTGA GTGCCGGGAG CTGCTGATGG ACTTCGCCAA TAGCAGCGCC 
GAGCTGACCG CCTGTATGGT GCGCAGCGCT CGGCCCGTGC GCCTCTGCCA GACCTGCTAC 
CCGCTCTTCC AACAGGTCGC AATCAAGATG GACAACATCA GCCGAAACAT CGGGAATACC 
TCCGAGGGCC CGCGCTGTGG CGGAAGTCTC CTGACGGCAG ACAGAATGCA GATAGTTCTC 
ATGGTCTCTG AGTTTTTCAA CAGCACGTGG CAGGAGGCGA ACTGCGCAAA TTGCCTAACA 
AACAATGGTG AGGATTTGTC AAACAACACA GAGGACTTCC TCAGTCTGTT TAACAAGACT 
TTGGCCTGCT TTGAGCATAA CCTGCAGGGG CACACATACA GTCTCCTCCC ACCAAAAAAT 
TACTCCGAAG TGTGCAGAAA CTGTAAAGAG GCATATAAAA ACCTGAGCCT CCTGTACAGT 
CAAATGCAGA AACTGAACGG GCTTGAGAAC AAGGCTGAGC CTGAGACGCA CTTGTGCATC 
GATGTGGAGG ATGCAATGAA CATTACTCGG AAGCTTTGGA GTCGAACCTT CAACTGTTCG 
GTCACCTGCA GCGACACGGT GTCCGTGGTT GCTGTGTCTG TGTTCATTCT CTTCCTGCCT 
GTCGTCTTCT ACCTCAGTAG CTTCCTTCAC TCAGAGCAAA AGAAACGCAA ACTCATTCTA 
CCCAAACGTC TCAAGTCGAG CACCAGTTTT GCCAACATTC AAGAAAATGC CACCTGA