Bsnd cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bsnd
Entrez Gene ID	140475
Full Name	barttin CLCNK type accessory beta subunit
General protein information	Preferred Names barttin CLCNK type accessory beta subunit Names barttin Bartter syndrome, infantile, with sensorineural deafness (Barttin)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_080458.2	NP_536706.2	barttin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BSND	XP_003434700.1
Xenopus tropicalis (tropical clawed frog)	bsnd	XP_002931511.1
Pan troglodytes (chimpanzee)	BSND	XP_001143070.2
Homo sapiens (human)	BSND	NP_476517.1
Bos taurus (cattle)	BSND	NP_001180013.1
Rattus norvegicus (Norway rat)	Bsnd	NP_620435.1
	BSND	XP_001114228.1
Mus musculus (house mouse)	Bsnd	NP_536706.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of ClC-K and barttin in low-potassium induced sodium-chloride cotransporter activation and hypertension in mouse kidney.
Nomura N, Shoda W, Wang Y, Mandai S, Furusho T, Takahashi D, Zeniya M, Sohara E, Rai T, Uchida S
Bioscience reports(2018 Jan)

ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development.
Rohacek AM, Bebee TW, Tilton RK, Radens CM, McDermott-Roe C, Peart N, Kaur M, Zaykaner M, Cieply B, Musunuru K, Barash Y, Germiller JA, Krantz ID, Carstens RP, Epstein DJ
Developmental cell43(3)318-331.e5(2017 11)

Molecular architecture underlying fluid absorption by the developing inner ear.
Honda K, Kim SH, Kelly MC, Burns JC, Constance L, Li X, Zhou F, Hoa M, Kelley MW, Wangemann P, Morell RJ, Griffith AJ
eLife6(2017 Oct)

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Street VA, Kujawa SG, Manichaikul A, Broman KW, Kallman JC, Shilling DJ, Iwata AJ, Robinson LC, Robbins CA, Li J, Liberman MC, Tempel BL
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)

Canonical Wnt signaling regulates the proliferative expansion and differentiation of fibrocytes in the murine inner ear.
Bohnenpoll T, Trowe MO, Wojahn I, Taketo MM, Petry M, Kispert A
Developmental biology391(1)54-65(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

induction of SGK1, CLC-K1 and barttin by high osmolarity and change in intracellular volume in distal renal tubular cells in vivo and in vitro
Title: High osmolality induces the kidney-specific chloride channel CLC-K1 by a serum and glucocorticoid-inducible kinase 1 MAPK pathway.

Bsnd(-/-) mice thus demonstrate a novel function of Cl(-) channels in generating the endocochlear potential and reveal the mechanism leading to deafness in human Bartter syndrome IV.
Title: Endocochlear potential depends on Cl- channels: mechanism underlying deafness in Bartter syndrome IV.

The following Bsnd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bsnd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09675	NM_080458.2 Latest version!	Mus musculus barttin CLCNK type accessory beta subunit (Bsnd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bsnd ( NM_080458.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_536706.2 Mus musculus barttin CLCNK type accessory beta subunit (Bsnd), mRNA.

Price & Availability↑

CloneID	OMu09675
Clone ID Related Accession (Same CDS sequence)	NM_080458.2
Accession Version	NM_080458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	barttin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052587.1 and AL954352.10. On Apr 8, 2006 this sequence version replaced NM_080458.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038287.1, AK052587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGATG AGAAGACCTT CCGAATCGGC TTCATCGTGC TGGGCCTCTT CCTGCTATCC 
CTGGGTACAT TCCTTATGAG CCACGATCGA CCCCAGGTCT ACGGCACCTT CTATGCCATG 
GGCAGCGTCA TGGTGATCGG GGGTGTCATC TGGAGCATGT GCCAATGCTA TCCTAAGATC 
ACCTTTGTGC CAGCAGACTC TGACTTCCAA GGCATCTTGT CCCCAAAGGC CCTGAGCCTG 
CTGGAGACCG GGCTTTCGGA GGTAAAGAGC CCCCAGCCCC CCTACGTCAG GCTGTGGGAA 
GAAGCCGCCT ATGACCAGAG CCTCCCAGAC TTCACTCACA TCCAAATGAA GGTCATGGGC 
TACAGTGAGG ACCCCAGGCC ACTGCTGGCC CCCGAGCTGA AGACAGGAGC CAGCAGTGTC 
AGGGAAGGCG AGCCTCGTAC TGCTCAGGCC TGGATGGAGG CTCCGGTGGT CGTCCATAGA 
GGGTCAGATG AGAACGAGGG AGAGAAATCC CATTCTCAGA GCAGCCCTTC AGTGGGTCCC 
CAAGGCTCTG CACCCTTGGC CTCATTCCAT GACGACCTGG ACGTGGGCTC CAGCGAAGGC 
AGCAGCCTGC AACCATCCCC CAACAGGGAT GAGCCACACC GGCAGGTGCC CTGGGCCAGC 
AGGGGTCCAC TGGATCGCTT CAGTGACTTT GCTCTGATTG ACGACACCCC TACATCTGAG 
GACACGGTCC TGGACGGGCA GGCACGGGAG GCAGCGCTAC CCAGAAAGCA GCAATGGTCC 
CTGAGGATGA AGGGGGAGAC TGTCCAGGCA CGCGCAGAAG AACCGGAGCA GGAGGAGGAA 
GATCTGTACT ACGGGCTGCC AGACAGCCCT GGGAACCCCC TACCCGACAA AGAGTTGGGC 
TTCGAGCCTG ACATCCAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536706.2
CDS	203..1126
Translation	MADEKTFRIGFIVLGLFLLSLGTFLMSHDRPQVYGTFYAMGSVMVIGGVIWSMCQCYPKITFVPADSDFQGILSPKALSLLETGLSEVKSPQPPYVRLWEEAAYDQSLPDFTHIQMKVMGYSEDPRPLLAPELKTGASSVREGEPRTAQAWMEAPVVVHRGSDENEGEKSHSQSSPSVGPQGSAPLASFHDDLDVGSSEGSSLQPSPNRDEPHRQVPWASRGPLDRFSDFALIDDTPTSEDTVLDGQAREAALPRKQQWSLRMKGETVQARAEEPEQEEEDLYYGLPDSPGNPLPDKELGFEPDIQG

Target ORF information:

RefSeq Version	NM_080458.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus barttin CLCNK type accessory beta subunit (Bsnd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080458.2

ORF Insert Sequence:

ATGGCTGATG AGAAGACCTT CCGAATCGGC TTCATCGTGC TGGGCCTCTT CCTGCTATCC 
CTGGGTACAT TCCTTATGAG CCACGATCGA CCCCAGGTCT ACGGCACCTT CTATGCCATG 
GGCAGCGTCA TGGTGATCGG GGGTGTCATC TGGAGCATGT GCCAATGCTA TCCTAAGATC 
ACCTTTGTGC CAGCAGACTC TGACTTCCAA GGCATCTTGT CCCCAAAGGC CCTGAGCCTG 
CTGGAGACCG GGCTTTCGGA GGTAAAGAGC CCCCAGCCCC CCTACGTCAG GCTGTGGGAA 
GAAGCCGCCT ATGACCAGAG CCTCCCAGAC TTCACTCACA TCCAAATGAA GGTCATGGGC 
TACAGTGAGG ACCCCAGGCC ACTGCTGGCC CCCGAGCTGA AGACAGGAGC CAGCAGTGTC 
AGGGAAGGCG AGCCTCGTAC TGCTCAGGCC TGGATGGAGG CTCCGGTGGT CGTCCATAGA 
GGGTCAGATG AGAACGAGGG AGAGAAATCC CATTCTCAGA GCAGCCCTTC AGTGGGTCCC 
CAAGGCTCTG CACCCTTGGC CTCATTCCAT GACGACCTGG ACGTGGGCTC CAGCGAAGGC 
AGCAGCCTGC AACCATCCCC CAACAGGGAT GAGCCACACC GGCAGGTGCC CTGGGCCAGC 
AGGGGTCCAC TGGATCGCTT CAGTGACTTT GCTCTGATTG ACGACACCCC TACATCTGAG 
GACACGGTCC TGGACGGGCA GGCACGGGAG GCAGCGCTAC CCAGAAAGCA GCAATGGTCC 
CTGAGGATGA AGGGGGAGAC TGTCCAGGCA CGCGCAGAAG AACCGGAGCA GGAGGAGGAA 
GATCTGTACT ACGGGCTGCC AGACAGCCCT GGGAACCCCC TACCCGACAA AGAGTTGGGC 
TTCGAGCCTG ACATCCAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of ClC-K and barttin in low-potassium induced sodium-chloride cotransporter activation and hypertension in mouse kidney.
Bioscience reports(2018 Jan)
Nomura N,Shoda W,Wang Y,Mandai S,Furusho T,Takahashi D,Zeniya M,Sohara E,Rai T,Uchida S

ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development.
Developmental cell43(3)318-331.e5(2017 11)
Rohacek AM,Bebee TW,Tilton RK,Radens CM,McDermott-Roe C,Peart N,Kaur M,Zaykaner M,Cieply B,Musunuru K,Barash Y,Germiller JA,Krantz ID,Carstens RP,Epstein DJ

Molecular architecture underlying fluid absorption by the developing inner ear.
eLife6(2017 Oct)
Honda K,Kim SH,Kelly MC,Burns JC,Constance L,Li X,Zhou F,Hoa M,Kelley MW,Wangemann P,Morell RJ,Griffith AJ

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)
Street VA,Kujawa SG,Manichaikul A,Broman KW,Kallman JC,Shilling DJ,Iwata AJ,Robinson LC,Robbins CA,Li J,Liberman MC,Tempel BL

Canonical Wnt signaling regulates the proliferative expansion and differentiation of fibrocytes in the murine inner ear.
Developmental biology391(1)54-65(2014 Jul)
Bohnenpoll T,Trowe MO,Wojahn I,Taketo MM,Petry M,Kispert A