Cyp7b1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp7b1
Entrez Gene ID	13123
Full Name	cytochrome P450, family 7, subfamily b, polypeptide 1
Synonyms	AW261589,D3Ertd552e,hct-1
General protein information	Preferred Names cytochrome P450, family 7, subfamily b, polypeptide 1 Names 25-hydroxycholesterol 7-alpha-hydroxylase cytochrome P450 7B1 cytochrome P450, 7b1 hippocampal transcript 1 protein oxysterol 7-alpha-hydroxylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 A1\|3 4.98 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007825.4	NP_031851.3	cytochrome P450 7B1
XM_006535383.3	XP_006535446.1	25-hydroxycholesterol 7-alpha-hydroxylase isoform X1
XM_006535384.2	XP_006535447.1	25-hydroxycholesterol 7-alpha-hydroxylase isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC570455	XP_699028.2
Canis lupus familiaris (dog)	CYP7B1	XP_005638074.1
Bos taurus (cattle)	CYP7B1	XP_002692741.1
Gallus gallus (chicken)	CYP7B1	XP_418276.4
Rattus norvegicus (Norway rat)	Cyp7b1	NP_062011.1
Homo sapiens (human)	CYP7B1	NP_004811.1
	LOC710188	XP_001098766.2
Xenopus tropicalis (tropical clawed frog)	cyp7b1	NP_001120511.1
Pan troglodytes (chimpanzee)	CYP7B1	XP_001159731.1
Mus musculus (house mouse)	Cyp7b1	NP_031851.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Ferrell JM, Boehme S, Li F, Chiang JY
Journal of lipid research57(7)1144-54(2016 07)

EBI2 augments Tfh cell fate by promoting interaction with IL-2-quenching dendritic cells.
Li J, Lu E, Yi T, Cyster JG
Nature533(7601)110-4(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings indicate that CYP7B1 and HSD3B7, as well as CH25H, have essential roles in controlling oxysterol production in lymphoid tissues.
Title: Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.

Increasing 27-hydroxycholesterol concentrations by genetic means leads to decrease bone mineral density.
Title: The endogenous selective estrogen receptor modulator 27-hydroxycholesterol is a negative regulator of bone homeostasis.

CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.
Title: CYP7B1-mediated metabolism of 5alpha-androstane-3alpha,17beta-diol (3alpha-Adiol): a novel pathway for potential regulation of the cellular levels of androgens and neurosteroids.

Data show that ten genes (Fsp27, Aqp4, Cd36, Ly6d, Scd1, Hsd3b5, Syt1, Cyp7b1, and Tff3) showed significant associations among their expressions and the degree of hepatic steatosis.
Title: Microarray analysis of hepatic gene expression identifies new genes involved in steatotic liver.

Identification of Cyp7b1 as a novel RORalpha (NR1F1) target gene and a functional cross-talk between RORalpha and liver X receptor (NR1H3).
Title: Identification of oxysterol 7alpha-hydroxylase (Cyp7b1) as a novel retinoid-related orphan receptor alpha (RORalpha) (NR1F1) target gene and a functional cross-talk between RORalpha and liver X receptor (NR1H3).

The following Cyp7b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp7b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13007	NM_007825.4 Latest version!	Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OMu13007	XM_006535383.3 Latest version!	Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OMu31310	XM_006535384.2 Latest version!	Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp7b1 ( NM_007825.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031851.3 Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), mRNA.

Price & Availability↑

CloneID	OMu13007
Clone ID Related Accession (Same CDS sequence)	NM_007825.4 , XM_006535383.3
Accession Version	NM_007825.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Mus musculus(house mouse)
Product	cytochrome P450 7B1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ065666.1, AK013034.1 and AK140100.1. On Nov 20, 2007 this sequence version replaced NM_007825.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK172152.1, U36993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGAG CCACGACCCT AGATGCCGCC TCGCCAGGGC CTCTCGCCCT CCTAGGCCTT 
CTCTTTGCCG CCACCTTACT GCTCTCGGCC CTGTTCCTCC TTACCCGGCG CACCAGGCGC 
CCTCGTGAAC CACCCTTGAT AAAAGGTTGG CTTCCTTATC TTGGCATGGC CCTGAAATTC 
TTTAAGGATC CGTTAACTTT CTTGAAAACT CTTCAAAGGC AACATGGTGA CACTTTCACT 
GTCTTCCTTG TGGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ACGAAAAACC CAAAACAATT AAGCTTTCAG AAGTTCAGCA GCCGATTATC AGCGAAAGCC 
TTCTCTGTAA AGAAGCTGCT TACTGATGAC GACCTTAATG AAGACGTTCA CAGAGCCTAT 
CTACTTCTAC AAGGCAAACC TTTGGATGCT CTTCTGGAAA CTATGATCCA AGAAGTAAAA 
GAATTATTTG AGTCCCAACT GCTAAAAATC ACAGATTGGA ACACAGAAAG AATATTTGCA 
TTCTGTGGCT CACTGGTATT TGAGATCACA TTTGCGACTC TATATGGAAA AATTCTTGCT 
GGTAACAAGA AACAAATTAT CAGTGAGCTA AGGGATGATT TTTTTAAATT TGATGACATG 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGAAGA ATCTATGCAG 
AAGAAAATTA TAAAATGCCT CACATCAGAA AAAGTAGCTC AGATGCAAGG ACAGTCAAAA 
ATTGTTCAGG AAAGGCAAGA TCTGCTGAAA AGATACTATA GGCATGACGA TCCTGAAATA 
GGAGCACATC ATCTTGGCTT TCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATATTCT TCGGCATCCT GAAGCTATGG AAGCCCTGCG TGACGAAATT 
GACAGTTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCAGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GAAAGCACTA TTCTTGAGGT TCTGAGGCTG 
TGCTCATACT CCAGCATCAT CCGAGAAGTG CAGGAGGATA TGAATCTCAG CTTAGAGAGT 
AAGAGTTTCT CTCTGCGGAA AGGAGATTTT GTAGCCCTCT TTCCTCCACT CATACACAAT 
GACCCGGAAA TCTTCGATGC TCCAAAGGAA TTTAGGTTCG ATCGCTTCAT AGAAGATGGT 
AAGAAGAAAA GCACGTTTTT CAAAGGAGGG AAGAAGCTGA AGACTTACGT TATGCCTTTT 
GGACTCGGAA CAAGCAAATG TCCAGGGAGA TATTTTGCAG TGAACGAAAT GAAGTTACTG 
CTGATTATGC TTTTAACTTA TTTTGATTTA GAAATTATCG ACAGGAAGCC TATAGGGCTA 
AATCACAGTC GGATGTTTTT AGGTATTCAG CACCCCGATT CTGCCGTCTC CTTTAGGTAC 
AAAGCAAAAT CTTGGAGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031851.3
CDS	136..1659
Translation	MQGATTLDAASPGPLALLGLLFAATLLLSALFLLTRRTRRPREPPLIKGWLPYLGMALKFFKDPLTFLKTLQRQHGDTFTVFLVGKYITFVLNPFQYQYVTKNPKQLSFQKFSSRLSAKAFSVKKLLTDDDLNEDVHRAYLLLQGKPLDALLETMIQEVKELFESQLLKITDWNTERIFAFCGSLVFEITFATLYGKILAGNKKQIISELRDDFFKFDDMFPYLVSDIPIQLLRNEESMQKKIIKCLTSEKVAQMQGQSKIVQERQDLLKRYYRHDDPEIGAHHLGFLWASLANTIPAMFWAMYYILRHPEAMEALRDEIDSFLQSTGQKKGPGISVHFTREQLDSLVCLESTILEVLRLCSYSSIIREVQEDMNLSLESKSFSLRKGDFVALFPPLIHNDPEIFDAPKEFRFDRFIEDGKKKSTFFKGGKKLKTYVMPFGLGTSKCPGRYFAVNEMKLLLIMLLTYFDLEIIDRKPIGLNHSRMFLGIQHPDSAVSFRYKAKSWRS

Target ORF information:

RefSeq Version	NM_007825.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007825.4

ORF Insert Sequence:

ATGCAGGGAG CCACGACCCT AGATGCCGCC TCGCCAGGGC CTCTCGCCCT CCTAGGCCTT 
CTCTTTGCCG CCACCTTACT GCTCTCGGCC CTGTTCCTCC TTACCCGGCG CACCAGGCGC 
CCTCGTGAAC CACCCTTGAT AAAAGGTTGG CTTCCTTATC TTGGCATGGC CCTGAAATTC 
TTTAAGGATC CGTTAACTTT CTTGAAAACT CTTCAAAGGC AACATGGTGA CACTTTCACT 
GTCTTCCTTG TGGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ACGAAAAACC CAAAACAATT AAGCTTTCAG AAGTTCAGCA GCCGATTATC AGCGAAAGCC 
TTCTCTGTAA AGAAGCTGCT TACTGATGAC GACCTTAATG AAGACGTTCA CAGAGCCTAT 
CTACTTCTAC AAGGCAAACC TTTGGATGCT CTTCTGGAAA CTATGATCCA AGAAGTAAAA 
GAATTATTTG AGTCCCAACT GCTAAAAATC ACAGATTGGA ACACAGAAAG AATATTTGCA 
TTCTGTGGCT CACTGGTATT TGAGATCACA TTTGCGACTC TATATGGAAA AATTCTTGCT 
GGTAACAAGA AACAAATTAT CAGTGAGCTA AGGGATGATT TTTTTAAATT TGATGACATG 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGAAGA ATCTATGCAG 
AAGAAAATTA TAAAATGCCT CACATCAGAA AAAGTAGCTC AGATGCAAGG ACAGTCAAAA 
ATTGTTCAGG AAAGGCAAGA TCTGCTGAAA AGATACTATA GGCATGACGA TCCTGAAATA 
GGAGCACATC ATCTTGGCTT TCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATATTCT TCGGCATCCT GAAGCTATGG AAGCCCTGCG TGACGAAATT 
GACAGTTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCAGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GAAAGCACTA TTCTTGAGGT TCTGAGGCTG 
TGCTCATACT CCAGCATCAT CCGAGAAGTG CAGGAGGATA TGAATCTCAG CTTAGAGAGT 
AAGAGTTTCT CTCTGCGGAA AGGAGATTTT GTAGCCCTCT TTCCTCCACT CATACACAAT 
GACCCGGAAA TCTTCGATGC TCCAAAGGAA TTTAGGTTCG ATCGCTTCAT AGAAGATGGT 
AAGAAGAAAA GCACGTTTTT CAAAGGAGGG AAGAAGCTGA AGACTTACGT TATGCCTTTT 
GGACTCGGAA CAAGCAAATG TCCAGGGAGA TATTTTGCAG TGAACGAAAT GAAGTTACTG 
CTGATTATGC TTTTAACTTA TTTTGATTTA GAAATTATCG ACAGGAAGCC TATAGGGCTA 
AATCACAGTC GGATGTTTTT AGGTATTCAG CACCCCGATT CTGCCGTCTC CTTTAGGTAC 
AAAGCAAAAT CTTGGAGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp7b1 ( XM_006535383.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535446.1 Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13007
Clone ID Related Accession (Same CDS sequence)	NM_007825.4 , XM_006535383.3
Accession Version	XM_006535383.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	25-hydroxycholesterol 7-alpha-hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535383.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGGAG CCACGACCCT AGATGCCGCC TCGCCAGGGC CTCTCGCCCT CCTAGGCCTT 
CTCTTTGCCG CCACCTTACT GCTCTCGGCC CTGTTCCTCC TTACCCGGCG CACCAGGCGC 
CCTCGTGAAC CACCCTTGAT AAAAGGTTGG CTTCCTTATC TTGGCATGGC CCTGAAATTC 
TTTAAGGATC CGTTAACTTT CTTGAAAACT CTTCAAAGGC AACATGGTGA CACTTTCACT 
GTCTTCCTTG TGGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ACGAAAAACC CAAAACAATT AAGCTTTCAG AAGTTCAGCA GCCGATTATC AGCGAAAGCC 
TTCTCTGTAA AGAAGCTGCT TACTGATGAC GACCTTAATG AAGACGTTCA CAGAGCCTAT 
CTACTTCTAC AAGGCAAACC TTTGGATGCT CTTCTGGAAA CTATGATCCA AGAAGTAAAA 
GAATTATTTG AGTCCCAACT GCTAAAAATC ACAGATTGGA ACACAGAAAG AATATTTGCA 
TTCTGTGGCT CACTGGTATT TGAGATCACA TTTGCGACTC TATATGGAAA AATTCTTGCT 
GGTAACAAGA AACAAATTAT CAGTGAGCTA AGGGATGATT TTTTTAAATT TGATGACATG 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGAAGA ATCTATGCAG 
AAGAAAATTA TAAAATGCCT CACATCAGAA AAAGTAGCTC AGATGCAAGG ACAGTCAAAA 
ATTGTTCAGG AAAGGCAAGA TCTGCTGAAA AGATACTATA GGCATGACGA TCCTGAAATA 
GGAGCACATC ATCTTGGCTT TCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATATTCT TCGGCATCCT GAAGCTATGG AAGCCCTGCG TGACGAAATT 
GACAGTTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCAGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GAAAGCACTA TTCTTGAGGT TCTGAGGCTG 
TGCTCATACT CCAGCATCAT CCGAGAAGTG CAGGAGGATA TGAATCTCAG CTTAGAGAGT 
AAGAGTTTCT CTCTGCGGAA AGGAGATTTT GTAGCCCTCT TTCCTCCACT CATACACAAT 
GACCCGGAAA TCTTCGATGC TCCAAAGGAA TTTAGGTTCG ATCGCTTCAT AGAAGATGGT 
AAGAAGAAAA GCACGTTTTT CAAAGGAGGG AAGAAGCTGA AGACTTACGT TATGCCTTTT 
GGACTCGGAA CAAGCAAATG TCCAGGGAGA TATTTTGCAG TGAACGAAAT GAAGTTACTG 
CTGATTATGC TTTTAACTTA TTTTGATTTA GAAATTATCG ACAGGAAGCC TATAGGGCTA 
AATCACAGTC GGATGTTTTT AGGTATTCAG CACCCCGATT CTGCCGTCTC CTTTAGGTAC 
AAAGCAAAAT CTTGGAGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535446.1
CDS	401..1924
Translation	MQGATTLDAASPGPLALLGLLFAATLLLSALFLLTRRTRRPREPPLIKGWLPYLGMALKFFKDPLTFLKTLQRQHGDTFTVFLVGKYITFVLNPFQYQYVTKNPKQLSFQKFSSRLSAKAFSVKKLLTDDDLNEDVHRAYLLLQGKPLDALLETMIQEVKELFESQLLKITDWNTERIFAFCGSLVFEITFATLYGKILAGNKKQIISELRDDFFKFDDMFPYLVSDIPIQLLRNEESMQKKIIKCLTSEKVAQMQGQSKIVQERQDLLKRYYRHDDPEIGAHHLGFLWASLANTIPAMFWAMYYILRHPEAMEALRDEIDSFLQSTGQKKGPGISVHFTREQLDSLVCLESTILEVLRLCSYSSIIREVQEDMNLSLESKSFSLRKGDFVALFPPLIHNDPEIFDAPKEFRFDRFIEDGKKKSTFFKGGKKLKTYVMPFGLGTSKCPGRYFAVNEMKLLLIMLLTYFDLEIIDRKPIGLNHSRMFLGIQHPDSAVSFRYKAKSWRS

Target ORF information:

RefSeq Version	XM_006535383.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535383.3

ORF Insert Sequence:

ATGCAGGGAG CCACGACCCT AGATGCCGCC TCGCCAGGGC CTCTCGCCCT CCTAGGCCTT 
CTCTTTGCCG CCACCTTACT GCTCTCGGCC CTGTTCCTCC TTACCCGGCG CACCAGGCGC 
CCTCGTGAAC CACCCTTGAT AAAAGGTTGG CTTCCTTATC TTGGCATGGC CCTGAAATTC 
TTTAAGGATC CGTTAACTTT CTTGAAAACT CTTCAAAGGC AACATGGTGA CACTTTCACT 
GTCTTCCTTG TGGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ACGAAAAACC CAAAACAATT AAGCTTTCAG AAGTTCAGCA GCCGATTATC AGCGAAAGCC 
TTCTCTGTAA AGAAGCTGCT TACTGATGAC GACCTTAATG AAGACGTTCA CAGAGCCTAT 
CTACTTCTAC AAGGCAAACC TTTGGATGCT CTTCTGGAAA CTATGATCCA AGAAGTAAAA 
GAATTATTTG AGTCCCAACT GCTAAAAATC ACAGATTGGA ACACAGAAAG AATATTTGCA 
TTCTGTGGCT CACTGGTATT TGAGATCACA TTTGCGACTC TATATGGAAA AATTCTTGCT 
GGTAACAAGA AACAAATTAT CAGTGAGCTA AGGGATGATT TTTTTAAATT TGATGACATG 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGAAGA ATCTATGCAG 
AAGAAAATTA TAAAATGCCT CACATCAGAA AAAGTAGCTC AGATGCAAGG ACAGTCAAAA 
ATTGTTCAGG AAAGGCAAGA TCTGCTGAAA AGATACTATA GGCATGACGA TCCTGAAATA 
GGAGCACATC ATCTTGGCTT TCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATATTCT TCGGCATCCT GAAGCTATGG AAGCCCTGCG TGACGAAATT 
GACAGTTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCAGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GAAAGCACTA TTCTTGAGGT TCTGAGGCTG 
TGCTCATACT CCAGCATCAT CCGAGAAGTG CAGGAGGATA TGAATCTCAG CTTAGAGAGT 
AAGAGTTTCT CTCTGCGGAA AGGAGATTTT GTAGCCCTCT TTCCTCCACT CATACACAAT 
GACCCGGAAA TCTTCGATGC TCCAAAGGAA TTTAGGTTCG ATCGCTTCAT AGAAGATGGT 
AAGAAGAAAA GCACGTTTTT CAAAGGAGGG AAGAAGCTGA AGACTTACGT TATGCCTTTT 
GGACTCGGAA CAAGCAAATG TCCAGGGAGA TATTTTGCAG TGAACGAAAT GAAGTTACTG 
CTGATTATGC TTTTAACTTA TTTTGATTTA GAAATTATCG ACAGGAAGCC TATAGGGCTA 
AATCACAGTC GGATGTTTTT AGGTATTCAG CACCCCGATT CTGCCGTCTC CTTTAGGTAC 
AAAGCAAAAT CTTGGAGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp7b1 ( XM_006535384.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535447.1 Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31310
Clone ID Related Accession (Same CDS sequence)	XM_006535384.2
Accession Version	XM_006535384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	25-hydroxycholesterol 7-alpha-hydroxylase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGGAG CCACGACCCT AGATGCCGCC TCGCCAGGGC CTCTCGCCCT CCTAGGCCTT 
CTCTTTGCCG CCACCTTACT GCTCTCGGCC CTGTTCCTCC TTACCCGGCG CACCAGGCGC 
CCTCGTGAAC CACCCTTGAT AAAAGGTTGG CTTCCTTATC TTGGCATGGC CCTGAAATTC 
TTTAAGGATC CGTTAACTTT CTTGAAAACT CTTCAAAGGC AACATGGTGA CACTTTCACT 
GTCTTCCTTG TGGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ACGAAAAACC CAAAACAATT AAGCTTTCAG AAGTTCAGCA GCCGATTATC AGCGAAAGCC 
TTCTCTGTAA AGAAGCTGCT TACTGATGAC GACCTTAATG AAGACGTTCA CAGAGCCTAT 
CTACTTCTAC AAGGCAAACC TTTGGATGCT CTTCTGGAAA CTATGATCCA AGAAGTAAAA 
GAATTATTTG AGTCCCAACT GCTAAAAATC ACAGATTGGA ACACAGAAAG AATATTTGCA 
TTCTGTGGCT CACTGGTATT TGAGATCACA TTTGCGACTC TATATGGAAA AATTCTTGCT 
GGTAACAAGA AACAAATTAT CAGTGAGCTA AGGGATGATT TTTTTAAATT TGATGACATG 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGAAGA ATCTATGCAG 
AAGAAAATTA TAAAATGCCT CACATCAGAA AAAGTAGCTC AGATGCAAGG ACAGTCAAAA 
ATTGTTCAGG AAAGGCAAGA TCTGCTGAAA AGATACTATA GGCATGACGA TCCTGAAATA 
GGAGCACATC ATCTTGGCTT TCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATATTCT TCGGCATCCT GAAGCTATGG AAGCCCTGCG TGACGAAATT 
GACAGTTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCAGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GGAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535447.1
CDS	401..1459
Translation	MQGATTLDAASPGPLALLGLLFAATLLLSALFLLTRRTRRPREPPLIKGWLPYLGMALKFFKDPLTFLKTLQRQHGDTFTVFLVGKYITFVLNPFQYQYVTKNPKQLSFQKFSSRLSAKAFSVKKLLTDDDLNEDVHRAYLLLQGKPLDALLETMIQEVKELFESQLLKITDWNTERIFAFCGSLVFEITFATLYGKILAGNKKQIISELRDDFFKFDDMFPYLVSDIPIQLLRNEESMQKKIIKCLTSEKVAQMQGQSKIVQERQDLLKRYYRHDDPEIGAHHLGFLWASLANTIPAMFWAMYYILRHPEAMEALRDEIDSFLQSTGQKKGPGISVHFTREQLDSLVCLGI

Target ORF information:

RefSeq Version	XM_006535384.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535384.2

ORF Insert Sequence:

ATGCAGGGAG CCACGACCCT AGATGCCGCC TCGCCAGGGC CTCTCGCCCT CCTAGGCCTT 
CTCTTTGCCG CCACCTTACT GCTCTCGGCC CTGTTCCTCC TTACCCGGCG CACCAGGCGC 
CCTCGTGAAC CACCCTTGAT AAAAGGTTGG CTTCCTTATC TTGGCATGGC CCTGAAATTC 
TTTAAGGATC CGTTAACTTT CTTGAAAACT CTTCAAAGGC AACATGGTGA CACTTTCACT 
GTCTTCCTTG TGGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ACGAAAAACC CAAAACAATT AAGCTTTCAG AAGTTCAGCA GCCGATTATC AGCGAAAGCC 
TTCTCTGTAA AGAAGCTGCT TACTGATGAC GACCTTAATG AAGACGTTCA CAGAGCCTAT 
CTACTTCTAC AAGGCAAACC TTTGGATGCT CTTCTGGAAA CTATGATCCA AGAAGTAAAA 
GAATTATTTG AGTCCCAACT GCTAAAAATC ACAGATTGGA ACACAGAAAG AATATTTGCA 
TTCTGTGGCT CACTGGTATT TGAGATCACA TTTGCGACTC TATATGGAAA AATTCTTGCT 
GGTAACAAGA AACAAATTAT CAGTGAGCTA AGGGATGATT TTTTTAAATT TGATGACATG 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGAAGA ATCTATGCAG 
AAGAAAATTA TAAAATGCCT CACATCAGAA AAAGTAGCTC AGATGCAAGG ACAGTCAAAA 
ATTGTTCAGG AAAGGCAAGA TCTGCTGAAA AGATACTATA GGCATGACGA TCCTGAAATA 
GGAGCACATC ATCTTGGCTT TCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATATTCT TCGGCATCCT GAAGCTATGG AAGCCCTGCG TGACGAAATT 
GACAGTTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCAGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GGAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Journal of lipid research57(7)1144-54(2016 07)
Ferrell JM,Boehme S,Li F,Chiang JY

EBI2 augments Tfh cell fate by promoting interaction with IL-2-quenching dendritic cells.
Nature533(7601)110-4(2016 May)
Li J,Lu E,Yi T,Cyster JG