Cyp21a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp21a1
Entrez Gene ID	13079
Full Name	cytochrome P450, family 21, subfamily a, polypeptide 1
Synonyms	21-OH,21OH,21OHA,21OHB,CYP21OH-A,Cyp21,Cyp21-ps1,Cyp21B,Cyp21a2-ps,Cyp21a2ps,Oh21-1,Oh21-2
General protein information	Preferred Names cytochrome P450, family 21, subfamily a, polypeptide 1 Names steroid 21-hydroxylase 21-OHase 21-hydroxylase cytochrome P-450 cytochrome P-450c21 cytochrome P450 21 cytochrome P450 XXI cytochrome P450 hydroxylase A cytochrome P450, 21, pseudogene cytochrome P450, 21, steroid 21 hydroxylase cytochrome P450, family 21, subfamily a, polypeptide 2, pseudogene cytochrome P450-C21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.36 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009995.2	NP_034125.2	steroid 21-hydroxylase precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CYP21A2	NP_000491.4
Xenopus tropicalis (tropical clawed frog)	LOC100489813	XP_002941314.2
Pan troglodytes (chimpanzee)	LOC745345	XP_003311237.1
Gallus gallus (chicken)	CYP21A2	NP_001092828.1
Mus musculus (house mouse)	Cyp21a1	NP_034125.2
Danio rerio (zebrafish)	LOC793249	XP_005157984.1
	CYP21A2	XP_001114240.1
Canis lupus familiaris (dog)	CYP21A2	NP_001003335.1
Rattus norvegicus (Norway rat)	Cyp21a1	NP_476442.2
Bos taurus (cattle)	CYP21	NP_001013614.1

Gene Ontology
Bibliography

Related articles in PubMed

Biology of the Adrenal Gland Cortex Obviates Effective Use of Adeno-associated Virus Vectors to Treat Hereditary Adrenal Disorders.
Markmann S, De BP, Reid J, Jose C, Rosenberg JB, Leopold P, Kaminsky SM, Sondhi D, Pagovich OE, Crystal RG
Human gene therapy(2018 Jan)

Age-dependent effects of Armc5 haploinsufficiency on adrenocortical function.
Berthon A, Faucz FR, Espiard S, Drougat L, Bertherat J, Stratakis CA
Human molecular genetics26(18)3495-3507(2017 Sep)

Dynamic modulation of Cyp21a1 (21-hydroxylase) expression sites in the mouse developing lung.
Gilbert C, Provost PR, Tremblay Y
The Journal of steroid biochemistry and molecular biology168102-109(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cyp21a1 remains expressed in the most distal structure of the developing lung even though these structures are changing, but its expression is not restricted to these areas.
Title: Dynamic modulation of Cyp21a1 (21-hydroxylase) expression sites in the mouse developing lung.

Extra-adrenal induction of Cyp21a1 ameliorates steroid metabolism in 21-OHD mice. This study suggests a novel therapeutic strategy for congenital adrenal hyperplasia, which warrants further investigations.
Title: Extra-adrenal induction of Cyp21a1 ameliorates systemic steroid metabolism in a mouse model of congenital adrenal hyperplasia.

Ureteral obstruction leads to the alteration of renal vitamin D metabolic enzyme expression and calcium transporter abundance, which may secondarily induce the abnormality of vitamin D endocrine system and bone health.
Title: Changes of renal vitamin D metabolic enzyme expression and calcium transporter abundance in obstructive nephropathy.

Allelic variability for 30-kb deletion in patients with 21-hydroxylase deficiency indicates that a founder effect may be improbable for most monomodular alleles carrying CYP21A1P/A2 chimeric genes in Brazil
Title: Novel deletion alleles carrying CYP21A1P/A2 chimeric genes in Brazilian patients with 21-hydroxylase deficiency.

The mutations are: I, no or minor decrease in enzyme activity: R238Q, P465L, R361K, A362V, P458L; II, loss of enzyme activity caused by inefficient electron flux: R346H, R400C; III, loss of activity due to deficient substrate binding: I462F, L464F
Title: Congenital adrenal hyperplasia: the molecular basis of 21-hydroxylase deficiency in H-2(aw18) mice.

The following Cyp21a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp21a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22001	NM_009995.2 Latest version!	Mus musculus cytochrome P450, family 21, subfamily a, polypeptide 1 (Cyp21a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$286.30 $409.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp21a1 ( NM_009995.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034125.2 Mus musculus cytochrome P450, family 21, subfamily a, polypeptide 1 (Cyp21a1), mRNA.

Price & Availability↑

CloneID	OMu22001
Clone ID Related Accession (Same CDS sequence)	NM_009995.2
Accession Version	NM_009995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	steroid 21-hydroxylase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK146565.1, BC127167.1, AI323066.1 and BQ033552.1. On Nov 22, 2007 this sequence version replaced NM_009995.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146565.1, BC127167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTAC CTGGGCTGCT GCTGCTGTTG CTGCTGCTAG CTGGCACCCG CTGGCTGTGG 
GGCCAATGGA AGCTCCGGAA GCTGCACCTC CCGCCTCTGG CCCCGGGTTT TCTGCACTTC 
CTACAGCCTA ACCTTCCCAT CTACCTGCTT GGCCTCACTC AGAAACTCGG GCCCATCTAC 
AGGATCCGCT TGGGGATGCA AGATGTGGTG GTGCTAAATT CTAACAGAAC CATTGAGGAG 
GCCTTGATCC AAAAGTGGGT GGACTTTGCT GGCCGACCCC ATATGCTAAA TGGAAAGATG 
GACTTGGACC TGTCCCTGGG GGATTACTCT CTCATGTGGA AGGCCCACAA GAAACTCTCT 
CGCTCAGCCC TGATGCTGGG CATGCGAGAC TCCATGGAGC CTCTGATAGA GCAGCTGACC 
CAGGAGTTCT GTGAGCGCAT GCGAGCCCAG GCTGGCACCC CCGTGGCCAT CCATAAGGAA 
TTCTCCTTCC TCACTTGTAG TATCATCTCC TGCCTCACTT TTGGAGACAA GGACAGCACG 
TTGGTACAGA CCCTTCACGA CTGTGTCCAG GACTTGTTGC AAGCCTGGAA CCACTGGTCC 
ATCCAAATCT TGACGATAAT TCCCCTTCTC AGGTTCCTCC CCAACCCAGG CCTCCAGAAG 
CTGAAGCAGA TCCAAGAGAG TCGGGACCAT ATTGTCAAGC AGCAGCTGAA GCGGCACAAG 
GACAGCCTGG TTGCAGGCCA ATGGAAAGAC ATGATTGACT ACATGCTCCA GGGAGTGGAG 
AAGCAAAGGG ATGGCAAAGA CGAAGAGCGG CTCCACGAGG GGCACGTGCA CATGTCGGTG 
GTGGACCTGT TCATCGGCGG CACCGAGACC ACGGCTACCA CGCTCTCCTG GGCTGTGGCT 
TTCCTGCTTC ACCACCCTGA GATCCAGAAG CGACTGCAGG AAGAGTTAGA CCTCAAGCTG 
GGCCCAGGCT CCCAGCTCCT GTACAGGAAC CGAATGCAGC TGCCTCTGCT CATGGCCACC 
ATTGCCGAGG TGCTGCGTTT GCGGCCTGTG GTGCCCTTGG CCTTGCCCCA TCGTGCAACT 
AGGGCTAGCA GCATCTCCGG CTATGACATC CCTAAGGATA TGGTCATCAT CCCCAACATC 
CAAGGCGCCA ACCTGGATGA GATGGTTTGG GAACTGCCCA GCAAGTTCTG GCCAGATCGC 
TTCCTGGAAC CTGGGAAGAA TCCCAGAACA CCATCCTTTG GCTGTGGGGC ACGCGTGTGC 
CTGGGAGAGC CTCTGGCACG GCTGGAGCTC TTTGTGGTGC TGGCTCGTCT GCTGCAGGCC 
TTCACTCTGC TGCCTCCACC AGATGGAACC CTGCCTTCCC TGCAGCCCCA GCCTTATGCT 
GGCATCAATC TCCCGATTCC TCCTTTCCAG GTGCGGCTGC AGCCCAGAAA CCTGGCGCCC 
CAAGACCAGG GTGAGCGTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034125.2
CDS	28..1491
Translation	MLLPGLLLLLLLLAGTRWLWGQWKLRKLHLPPLAPGFLHFLQPNLPIYLLGLTQKLGPIYRIRLGMQDVVVLNSNRTIEEALIQKWVDFAGRPHMLNGKMDLDLSLGDYSLMWKAHKKLSRSALMLGMRDSMEPLIEQLTQEFCERMRAQAGTPVAIHKEFSFLTCSIISCLTFGDKDSTLVQTLHDCVQDLLQAWNHWSIQILTIIPLLRFLPNPGLQKLKQIQESRDHIVKQQLKRHKDSLVAGQWKDMIDYMLQGVEKQRDGKDEERLHEGHVHMSVVDLFIGGTETTATTLSWAVAFLLHHPEIQKRLQEELDLKLGPGSQLLYRNRMQLPLLMATIAEVLRLRPVVPLALPHRATRASSISGYDIPKDMVIIPNIQGANLDEMVWELPSKFWPDRFLEPGKNPRTPSFGCGARVCLGEPLARLELFVVLARLLQAFTLLPPPDGTLPSLQPQPYAGINLPIPPFQVRLQPRNLAPQDQGERP

Target ORF information:

RefSeq Version	NM_009995.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 21, subfamily a, polypeptide 1 (Cyp21a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009995.2

ORF Insert Sequence:

ATGCTGCTAC CTGGGCTGCT GCTGCTGTTG CTGCTGCTAG CTGGCACCCG CTGGCTGTGG 
GGCCAATGGA AGCTCCGGAA GCTGCACCTC CCGCCTCTGG CCCCGGGTTT TCTGCACTTC 
CTACAGCCTA ACCTTCCCAT CTACCTGCTT GGCCTCACTC AGAAACTCGG GCCCATCTAC 
AGGATCCGCT TGGGGATGCA AGATGTGGTG GTGCTAAATT CTAACAGAAC CATTGAGGAG 
GCCTTGATCC AAAAGTGGGT GGACTTTGCT GGCCGACCCC ATATGCTAAA TGGAAAGATG 
GACTTGGACC TGTCCCTGGG GGATTACTCT CTCATGTGGA AGGCCCACAA GAAACTCTCT 
CGCTCAGCCC TGATGCTGGG CATGCGAGAC TCCATGGAGC CTCTGATAGA GCAGCTGACC 
CAGGAGTTCT GTGAGCGCAT GCGAGCCCAG GCTGGCACCC CCGTGGCCAT CCATAAGGAA 
TTCTCCTTCC TCACTTGTAG TATCATCTCC TGCCTCACTT TTGGAGACAA GGACAGCACG 
TTGGTACAGA CCCTTCACGA CTGTGTCCAG GACTTGTTGC AAGCCTGGAA CCACTGGTCC 
ATCCAAATCT TGACGATAAT TCCCCTTCTC AGGTTCCTCC CCAACCCAGG CCTCCAGAAG 
CTGAAGCAGA TCCAAGAGAG TCGGGACCAT ATTGTCAAGC AGCAGCTGAA GCGGCACAAG 
GACAGCCTGG TTGCAGGCCA ATGGAAAGAC ATGATTGACT ACATGCTCCA GGGAGTGGAG 
AAGCAAAGGG ATGGCAAAGA CGAAGAGCGG CTCCACGAGG GGCACGTGCA CATGTCGGTG 
GTGGACCTGT TCATCGGCGG CACCGAGACC ACGGCTACCA CGCTCTCCTG GGCTGTGGCT 
TTCCTGCTTC ACCACCCTGA GATCCAGAAG CGACTGCAGG AAGAGTTAGA CCTCAAGCTG 
GGCCCAGGCT CCCAGCTCCT GTACAGGAAC CGAATGCAGC TGCCTCTGCT CATGGCCACC 
ATTGCCGAGG TGCTGCGTTT GCGGCCTGTG GTGCCCTTGG CCTTGCCCCA TCGTGCAACT 
AGGGCTAGCA GCATCTCCGG CTATGACATC CCTAAGGATA TGGTCATCAT CCCCAACATC 
CAAGGCGCCA ACCTGGATGA GATGGTTTGG GAACTGCCCA GCAAGTTCTG GCCAGATCGC 
TTCCTGGAAC CTGGGAAGAA TCCCAGAACA CCATCCTTTG GCTGTGGGGC ACGCGTGTGC 
CTGGGAGAGC CTCTGGCACG GCTGGAGCTC TTTGTGGTGC TGGCTCGTCT GCTGCAGGCC 
TTCACTCTGC TGCCTCCACC AGATGGAACC CTGCCTTCCC TGCAGCCCCA GCCTTATGCT 
GGCATCAATC TCCCGATTCC TCCTTTCCAG GTGCGGCTGC AGCCCAGAAA CCTGGCGCCC 
CAAGACCAGG GTGAGCGTCC TTGA