Chkb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chkb
Entrez Gene ID	12651
Full Name	choline kinase beta
Synonyms	Chetk,Chkl,Ck,Ck/Ek,Ck/Ek-beta,Ckb,Ckekb,Ek
General protein information	Preferred Names choline kinase beta Names choline/ethanolamine kinase choline/ethanolamine kinase-b choline/ethanolaminekinase ethanolamine kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_007692.6	NP_031718.1	choline/ethanolamine kinase

Pathways from BioSystems

Homologies

	LOC716377	XP_002808018.1
Canis lupus familiaris (dog)	CHKB	XP_848257.1
Drosophila melanogaster (fruit fly)	CG2201	NP_724349.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007957	XP_317515.4
Oryza sativa (rice)	Os05g0535400	NP_001174502.1
Xenopus tropicalis (tropical clawed frog)	chkb	NP_001120148.1
Pan troglodytes (chimpanzee)	CHKB	XP_001144556.1
Bos taurus (cattle)	CHKB	NP_001193023.1
Caenorhabditis elegans (roundworm)	cka-1	NP_001129849.1
Rattus norvegicus (Norway rat)	Chkb	NP_058873.1
Danio rerio (zebrafish)	chkb	NP_001093482.1
Homo sapiens (human)	CHKB	NP_005189.2
Mus musculus (house mouse)	Chkb	NP_031718.1
Arabidopsis thaliana (thale cress)	AT1G74320	NP_177572.2
Oryza sativa (rice)	Os01g0717000	NP_001044073.1

Gene Ontology
Bibliography

Related articles in PubMed

Choline kinase ? mutant mice exhibit reduced phosphocholine, elevated osteoclast activity, and low bone mass.
Kular J, Tickner JC, Pavlos NJ, Viola HM, Abel T, Lim BS, Yang X, Chen H, Cook R, Hool LC, Zheng MH, Xu J
The Journal of biological chemistry290(3)1729-42(2015 Jan)

Choline kinase beta is required for normal endochondral bone formation.
Li Z, Wu G, Sher RB, Khavandgar Z, Hermansson M, Cox GA, Doschak MR, Murshed M, Beier F, Vance DE
Biochimica et biophysica acta1840(7)2112-22(2014 Jul)

Muscle choline kinase beta defect causes mitochondrial dysfunction and increased mitophagy.
Mitsuhashi S, Hatakeyama H, Karahashi M, Koumura T, Nonaka I, Hayashi YK, Noguchi S, Sher RB, Nakagawa Y, Manfredi G, Goto Y, Cox GA, Nishino I
Human molecular genetics20(19)3841-51(2011 Oct)

A congenital muscular dystrophy with mitochondrial structural abnormalities caused by defective de novo phosphatidylcholine biosynthesis.
Mitsuhashi S, Ohkuma A, Talim B, Karahashi M, Koumura T, Aoyama C, Kurihara M, Quinlivan R, Sewry C, Mitsuhashi H, Goto K, Koksal B, Kale G, Ikeda K, Taguchi R, Noguchi S, Hayashi YK, Nonaka I, Sher RB, Sugimoto H, Nakagawa Y, Cox GA, Topaloglu H, Nishino I
American journal of human genetics88(6)845-851(2011 Jun)

The gene encoding muscle-type carnitine palmitoyltransferase I: comparison of the 5'-upstream region of human and rodent genes.
Yamazaki N, Yamanaka Y, Hashimoto Y, Hiramatsu T, Shinohara Y, Terada H
Journal of biochemistry133(4)523-32(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CHKB is a new modulator of bone homeostasis
Title: Choline kinase ? mutant mice exhibit reduced phosphocholine, elevated osteoclast activity, and low bone mass.

Our data indicate that choline kinase beta plays an important role in endochondral bone formation by modulating growth plate physiology.
Title: Choline kinase beta is required for normal endochondral bone formation.

Loss of choline kinase beta in skeletal muscle causes mitochondrial dysfunction and increased mitophagy.
Title: Muscle choline kinase beta defect causes mitochondrial dysfunction and increased mitophagy.

The hindlimb muscular dystrophy in Chkb -/- mice is due to attenuated phosphatidylcholine biosynthesis and enhanced catabolism of phosphatidylcholine.
Title: Understanding the muscular dystrophy caused by deletion of choline kinase beta in mice.

Mutation of the corresponding D276 of CK-beta to A276 did not show any effect on either its homo- or hetero-dimerization but it caused a strong inhibition of CK activity in either case.
Title: Deletion and alanine mutation analyses for the formation of active homo- or hetero-dimer complexes of mouse choline kinase-alpha and -beta.

The following Chkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00619	NM_007692.6 Latest version!	Mus musculus choline kinase beta (Chkb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chkb ( NM_007692.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031718.1 Mus musculus choline kinase beta (Chkb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00619
Clone ID Related Accession (Same CDS sequence)	NM_007692.6
Accession Version	NM_007692.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	choline/ethanolamine kinase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK078101.1, AC137513.3 and AI853330.1. On Nov 4, 2010 this sequence version replaced NM_007692.5. Transcript Variant: This variant (1) represents the shortest transcript but encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK078101.1, AB011000.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG ACGGGACAGG GGTAGTCGGA GGAGGGGCTG TCGGCGGCGG CCTGCCCAAG 
GACGGTTTGC AGGATGCTAA GTGCCCAGAG CCAATCCCCA ATCGGCGGCG CGCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGGGCC 
TGGCGCCGAG CGCGGCCGGA GGAGCTGAGC GTTTGTCCCG TGAGCGGAGG CCTCAGCAAC 
CTGCTCTTCC GATGCTCACT ACCGAACCAC GTACCCAGTG TGGGCGGGGA GCCCCGGGAG 
GTGCTGCTAC GACTCTACGG GGCCATCCTG CAGGGTGTAG ACTCCTTGGT ATTAGAAAGC 
GTGATGTTCG CCATTCTCGC AGAGCGGTCT CTAGGGCCCC AGCTTTACGG AGTGTTTCCA 
GAGGGCCGCT TGGAACAGTA CCTCCCAAGC CGGCCGTTGA AAACTCAAGA GCTCCGGGAC 
CCAGTGTTGT CGGGAGCCAT TGCAACGAGG ATGGCCCGTT TCCATGGTAT GGAGATGCCC 
TTCACTAAGG AGCCCCGATG GTTGTTTGGG ACCATGGAGC GGTACCTGAA GCAGATCCAG 
GACTTGCCTT CCACCAGCCT TCCCCAGATG AACCTGGTAG AGATGTACAG CCTCAAGGAC 
GAGATGAACA GCCTCAGGAA GTTACTAGAC GATACCCCGT CACCAGTGGT CTTCTGCCAC 
AATGACATCC AGGAAGGAAA CATCTTGTTG CTTTCAGAGC CAGACAGTGA TGATAACCTC 
ATGTTGGTTG ATTTTGAGTA CAGTAGTTAT AACTACAGGG GCTTTGACAT TGGGAATCAT 
TTTTGTGAGT GGGTTTATGA TTATACTTAT GAAGAATGGC CTTTCTACAA AGCAAGACCC 
ACGGACTACC CCACTAGAGA ACAGCAGCTC CATTTTATTC GCCATTATCT GGCAGAGGTT 
CAGAAGGGTG AGATCCTCTC CGAAGAGGAA CAGAAGAAAC GGGAAGAAGA ATTGCTGCTA 
GAGATCAGTC GGTACTCCCT GGCATCTCAC TTTTTCTGGG GTCTGTGGTC CACCCTGCAG 
GCTTCGATGT CCACTATAGA GTTCGGCTAC TTGGAGTATG CCCAATCTCG GTTCCAGTTC 
TACTTCCAGC AGAAGGGCCA GCTGACGAGT TCCCCATCAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031718.1
CDS	328..1512
Translation	MAADGTGVVGGGAVGGGLPKDGLQDAKCPEPIPNRRRASSLSRDAQRRAYQWCREYLGGAWRRARPEELSVCPVSGGLSNLLFRCSLPNHVPSVGGEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYLPSRPLKTQELRDPVLSGAIATRMARFHGMEMPFTKEPRWLFGTMERYLKQIQDLPSTSLPQMNLVEMYSLKDEMNSLRKLLDDTPSPVVFCHNDIQEGNILLLSEPDSDDNLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTYEEWPFYKARPTDYPTREQQLHFIRHYLAEVQKGEILSEEEQKKREEELLLEISRYSLASHFFWGLWSTLQASMSTIEFGYLEYAQSRFQFYFQQKGQLTSSPSS

Target ORF information:

RefSeq Version	NM_007692.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus choline kinase beta (Chkb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007692.6

ORF Insert Sequence:

ATGGCGGCAG ACGGGACAGG GGTAGTCGGA GGAGGGGCTG TCGGCGGCGG CCTGCCCAAG 
GACGGTTTGC AGGATGCTAA GTGCCCAGAG CCAATCCCCA ATCGGCGGCG CGCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGGGCC 
TGGCGCCGAG CGCGGCCGGA GGAGCTGAGC GTTTGTCCCG TGAGCGGAGG CCTCAGCAAC 
CTGCTCTTCC GATGCTCACT ACCGAACCAC GTACCCAGTG TGGGCGGGGA GCCCCGGGAG 
GTGCTGCTAC GACTCTACGG GGCCATCCTG CAGGGTGTAG ACTCCTTGGT ATTAGAAAGC 
GTGATGTTCG CCATTCTCGC AGAGCGGTCT CTAGGGCCCC AGCTTTACGG AGTGTTTCCA 
GAGGGCCGCT TGGAACAGTA CCTCCCAAGC CGGCCGTTGA AAACTCAAGA GCTCCGGGAC 
CCAGTGTTGT CGGGAGCCAT TGCAACGAGG ATGGCCCGTT TCCATGGTAT GGAGATGCCC 
TTCACTAAGG AGCCCCGATG GTTGTTTGGG ACCATGGAGC GGTACCTGAA GCAGATCCAG 
GACTTGCCTT CCACCAGCCT TCCCCAGATG AACCTGGTAG AGATGTACAG CCTCAAGGAC 
GAGATGAACA GCCTCAGGAA GTTACTAGAC GATACCCCGT CACCAGTGGT CTTCTGCCAC 
AATGACATCC AGGAAGGAAA CATCTTGTTG CTTTCAGAGC CAGACAGTGA TGATAACCTC 
ATGTTGGTTG ATTTTGAGTA CAGTAGTTAT AACTACAGGG GCTTTGACAT TGGGAATCAT 
TTTTGTGAGT GGGTTTATGA TTATACTTAT GAAGAATGGC CTTTCTACAA AGCAAGACCC 
ACGGACTACC CCACTAGAGA ACAGCAGCTC CATTTTATTC GCCATTATCT GGCAGAGGTT 
CAGAAGGGTG AGATCCTCTC CGAAGAGGAA CAGAAGAAAC GGGAAGAAGA ATTGCTGCTA 
GAGATCAGTC GGTACTCCCT GGCATCTCAC TTTTTCTGGG GTCTGTGGTC CACCCTGCAG 
GCTTCGATGT CCACTATAGA GTTCGGCTAC TTGGAGTATG CCCAATCTCG GTTCCAGTTC 
TACTTCCAGC AGAAGGGCCA GCTGACGAGT TCCCCATCAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Choline kinase ? mutant mice exhibit reduced phosphocholine, elevated osteoclast activity, and low bone mass.
The Journal of biological chemistry290(3)1729-42(2015 Jan)
Kular J,Tickner JC,Pavlos NJ,Viola HM,Abel T,Lim BS,Yang X,Chen H,Cook R,Hool LC,Zheng MH,Xu J

Choline kinase beta is required for normal endochondral bone formation.
Biochimica et biophysica acta1840(7)2112-22(2014 Jul)
Li Z,Wu G,Sher RB,Khavandgar Z,Hermansson M,Cox GA,Doschak MR,Murshed M,Beier F,Vance DE

Muscle choline kinase beta defect causes mitochondrial dysfunction and increased mitophagy.
Human molecular genetics20(19)3841-51(2011 Oct)
Mitsuhashi S,Hatakeyama H,Karahashi M,Koumura T,Nonaka I,Hayashi YK,Noguchi S,Sher RB,Nakagawa Y,Manfredi G,Goto Y,Cox GA,Nishino I

A congenital muscular dystrophy with mitochondrial structural abnormalities caused by defective de novo phosphatidylcholine biosynthesis.
American journal of human genetics88(6)845-851(2011 Jun)
Mitsuhashi S,Ohkuma A,Talim B,Karahashi M,Koumura T,Aoyama C,Kurihara M,Quinlivan R,Sewry C,Mitsuhashi H,Goto K,Koksal B,Kale G,Ikeda K,Taguchi R,Noguchi S,Hayashi YK,Nonaka I,Sher RB,Sugimoto H,Nakagawa Y,Cox GA,Topaloglu H,Nishino I

The gene encoding muscle-type carnitine palmitoyltransferase I: comparison of the 5'-upstream region of human and rodent genes.
Journal of biochemistry133(4)523-32(2003 Apr)
Yamazaki N,Yamanaka Y,Hashimoto Y,Hiramatsu T,Shinohara Y,Terada H