Ang2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ang2
Entrez Gene ID	11731
Full Name	angiogenin, ribonuclease A family, member 2
Synonyms	Angrp,Raa3,Rnase5b
General protein information	Preferred Names angiogenin, ribonuclease A family, member 2 Names angiogenin-2 angiogenin related protein ribonuclease A a3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_007449.2	NP_031475.2	angiogenin-2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages.
Peterson TE, Kirkpatrick ND, Huang Y, Farrar CT, Marijt KA, Kloepper J, Datta M, Amoozgar Z, Seano G, Jung K, Kamoun WS, Vardam T, Snuderl M, Goveia J, Chatterjee S, Batista A, Muzikansky A, Leow CC, Xu L, Batchelor TT, Duda DG, Fukumura D, Jain RK
Proceedings of the National Academy of Sciences of the United States of America113(16)4470-5(2016 Apr)

Synergistic actions of blocking angiopoietin-2 and tumor necrosis factor-? in suppressing remodeling of blood vessels and lymphatics in airway inflammation.
Le CT, Laidlaw G, Morehouse CA, Naiman B, Brohawn P, Mustelin T, Connor JR, McDonald DM
The American journal of pathology185(11)2949-68(2015 Nov)

Heterozygous expression of the oncogenic Pik3ca(H1047R) mutation during murine development results in fatal embryonic and extraembryonic defects.
Hare LM, Schwarz Q, Wiszniak S, Gurung R, Montgomery KG, Mitchell CA, Phillips WA
Developmental biology404(1)14-26(2015 Aug)

Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2.
Choi HJ, Zhang H, Park H, Choi KS, Lee HW, Agrawal V, Kim YM, Kwon YG
Nature communications66943(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dual inhibition of VEGFR/Ang-2 prolongs survival in preclinical GBM models by reducing tumor burden, improving normalization, and altering TAMs
Title: Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages.

Ang2 and TNF are essential and synergistic drivers of remodeling of blood vessels and lymphatics during the initial stage of inflammation after infection. Inhibition of Ang2 and TNF together results in widespread suppression of the inflammatory response.
Title: Synergistic actions of blocking angiopoietin-2 and tumor necrosis factor-? in suppressing remodeling of blood vessels and lymphatics in airway inflammation.

These findings implicate YAP as a critical regulator in angiogenesis and provide new insights into the mechanism coordinating junctional stability and angiogenic activation of endothelial cells.
Title: Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2.

Sonic hedgehog secreted by neurons regulates ang-1 and ang-2 expression in neighboring fibroblasts.
Title: Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts.

Ang2 is secreted from endothelial cells via exosomes and that this process is inhibited by the PI3K/Akt1/endothelial nitric oxide synthase (eNOS) signaling pathway.
Title: Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways.

The following Ang2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ang2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18017	NM_007449.2 Latest version!	Mus musculus angiogenin, ribonuclease A family, member 2 (Ang2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ang2 ( NM_007449.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031475.2 Mus musculus angiogenin, ribonuclease A family, member 2 (Ang2), mRNA.

Price & Availability↑

CloneID	OMu18017
Clone ID Related Accession (Same CDS sequence)	NM_007449.2
Accession Version	NM_007449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	angiogenin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163664.3. On Aug 21, 2006 this sequence version replaced NM_007449.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCGATGA GCCCAGGTCC TTTGTTCTTG GTCTTCCTGT TGGGTCTGGT TGTGATCCCT 
CCCACTCTGT CTCAGGATGA CTCCAGGTAC ACAAAATTCC TGACTCAGCA CTATGATGCC 
AAGCCAAAAG GCCGGGACGA CAGATACTGC GAAAGTATGA TGGTGAAAAG AAAGCTAACC 
TCTTTCTGCA AAGATGTCAA CACCTTTATC CATGACACCA AGAACAACAT CAAGGCCATC 
TGTGGAAAGA AAGGAAGCCC TTATGGACGA AATTTAAGAA TAAGCAAGTC TCACTTCCAG 
GTCACCACTT GCACACACAA AGGAAGGTCT CCCCGGCCTC CATGCAGGTA CCGAGCCTCT 
AAAGGGTTCA GATATATTAT TATTGGCTGT GAGAATGGCT GGCCTGTCCA CTTTGATGAG 
TCTTTTATCA GTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031475.2
CDS	1..438
Translation	MAMSPGPLFLVFLLGLVVIPPTLSQDDSRYTKFLTQHYDAKPKGRDDRYCESMMVKRKLTSFCKDVNTFIHDTKNNIKAICGKKGSPYGRNLRISKSHFQVTTCTHKGRSPRPPCRYRASKGFRYIIIGCENGWPVHFDESFISP

Target ORF information:

RefSeq Version	NM_007449.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus angiogenin, ribonuclease A family, member 2 (Ang2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007449.2

ORF Insert Sequence:

ATGGCGATGA GCCCAGGTCC TTTGTTCTTG GTCTTCCTGT TGGGTCTGGT TGTGATCCCT 
CCCACTCTGT CTCAGGATGA CTCCAGGTAC ACAAAATTCC TGACTCAGCA CTATGATGCC 
AAGCCAAAAG GCCGGGACGA CAGATACTGC GAAAGTATGA TGGTGAAAAG AAAGCTAACC 
TCTTTCTGCA AAGATGTCAA CACCTTTATC CATGACACCA AGAACAACAT CAAGGCCATC 
TGTGGAAAGA AAGGAAGCCC TTATGGACGA AATTTAAGAA TAAGCAAGTC TCACTTCCAG 
GTCACCACTT GCACACACAA AGGAAGGTCT CCCCGGCCTC CATGCAGGTA CCGAGCCTCT 
AAAGGGTTCA GATATATTAT TATTGGCTGT GAGAATGGCT GGCCTGTCCA CTTTGATGAG 
TCTTTTATCA GTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages.
Proceedings of the National Academy of Sciences of the United States of America113(16)4470-5(2016 Apr)
Peterson TE,Kirkpatrick ND,Huang Y,Farrar CT,Marijt KA,Kloepper J,Datta M,Amoozgar Z,Seano G,Jung K,Kamoun WS,Vardam T,Snuderl M,Goveia J,Chatterjee S,Batista A,Muzikansky A,Leow CC,Xu L,Batchelor TT,Duda DG,Fukumura D,Jain RK

Synergistic actions of blocking angiopoietin-2 and tumor necrosis factor-? in suppressing remodeling of blood vessels and lymphatics in airway inflammation.
The American journal of pathology185(11)2949-68(2015 Nov)
Le CT,Laidlaw G,Morehouse CA,Naiman B,Brohawn P,Mustelin T,Connor JR,McDonald DM

Heterozygous expression of the oncogenic Pik3ca(H1047R) mutation during murine development results in fatal embryonic and extraembryonic defects.
Developmental biology404(1)14-26(2015 Aug)
Hare LM,Schwarz Q,Wiszniak S,Gurung R,Montgomery KG,Mitchell CA,Phillips WA

Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2.
Nature communications66943(2015 May)
Choi HJ,Zhang H,Park H,Choi KS,Lee HW,Agrawal V,Kim YM,Kwon YG