Sptssa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptssa
Entrez Gene ID	104725
Full Name	serine palmitoyltransferase, small subunit A
Synonyms	1110002B05Rik,AA407909,AA409588,AU041967,Ssspta
General protein information	Preferred Names serine palmitoyltransferase, small subunit A Names serine palmitoyltransferase small subunit A small subunit of serine palmitoyltransferase A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134054.2	NP_598815.2	serine palmitoyltransferase small subunit A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPTSSA	NP_001181971.1
Danio rerio (zebrafish)	sptssa	NP_001087196.1
Xenopus tropicalis (tropical clawed frog)	sptssa	NP_001005001.1
Homo sapiens (human)	SPTSSA	NP_612145.2
	SPTSSA	XP_001115197.1
Mus musculus (house mouse)	Sptssa	NP_598815.2
Rattus norvegicus (Norway rat)	Sptssa	NP_001041208.1
Gallus gallus (chicken)	SPTSSA	XP_421234.2
Pan troglodytes (chimpanzee)	SPTSSA	XP_003314342.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

ORMDL/serine palmitoyltransferase stoichiometry determines effects of ORMDL3 expression on sphingolipid biosynthesis.
Siow D, Sunkara M, Dunn TM, Morris AJ, Wattenberg B
Journal of lipid research56(4)898-908(2015 Apr)

Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil.
Lau CG, Marikawa Y
Molecular reproduction and development81(11)994-1008(2014 Nov)

A hybrid photoreceptor expressing both rod and cone genes in a mouse model of enhanced S-cone syndrome.
Corbo JC, Cepko CL
PLoS genetics1(2)e11(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sptssa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptssa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10486	NM_134054.2 Latest version!	Mus musculus serine palmitoyltransferase, small subunit A (Sptssa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptssa ( NM_134054.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598815.2 Mus musculus serine palmitoyltransferase, small subunit A (Sptssa), mRNA.

Price & Availability↑

CloneID	OMu10486
Clone ID Related Accession (Same CDS sequence)	NM_134054.2
Accession Version	NM_134054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	serine palmitoyltransferase small subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY072251.1, AK003171.1 and BG091867.1. On Sep 7, 2006 this sequence version replaced NM_134054.1. ##Evidence-Data-START## Transcript exon combination :: BY093376.1, AK137543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGCA TGGCGCTGGC GCGAGCATGG AAGCAGATGT CCTGGTTCTA CTACCAGTAC 
CTGCTGGTCA CTGCGCTCTA CATGCTGGAG CCCTGGGAGC GAACCGTGTT CAATTCGATG 
CTGGTTTCCG TGGTGGGGAT GGCCCTGTAC ACTGGCTACG TCTTCATGCC CCAGCACATC 
ATGGCTATTC TGCATTACTT TGAAATTGTA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598815.2
CDS	90..305
Translation	MAGMALARAWKQMSWFYYQYLLVTALYMLEPWERTVFNSMLVSVVGMALYTGYVFMPQHIMAILHYFEIVQ

Target ORF information:

RefSeq Version	NM_134054.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine palmitoyltransferase, small subunit A (Sptssa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134054.2

ORF Insert Sequence:

ATGGCGGGCA TGGCGCTGGC GCGAGCATGG AAGCAGATGT CCTGGTTCTA CTACCAGTAC 
CTGCTGGTCA CTGCGCTCTA CATGCTGGAG CCCTGGGAGC GAACCGTGTT CAATTCGATG 
CTGGTTTCCG TGGTGGGGAT GGCCCTGTAC ACTGGCTACG TCTTCATGCC CCAGCACATC 
ATGGCTATTC TGCATTACTT TGAAATTGTA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

ORMDL/serine palmitoyltransferase stoichiometry determines effects of ORMDL3 expression on sphingolipid biosynthesis.
Journal of lipid research56(4)898-908(2015 Apr)
Siow D,Sunkara M,Dunn TM,Morris AJ,Wattenberg B

Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil.
Molecular reproduction and development81(11)994-1008(2014 Nov)
Lau CG,Marikawa Y

A hybrid photoreceptor expressing both rod and cone genes in a mouse model of enhanced S-cone syndrome.
PLoS genetics1(2)e11(2005 Aug)
Corbo JC,Cepko CL