Bckdk cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bckdk
Entrez Gene ID	110297841
Full Name	branched chain ketoacid dehydrogenase kinase
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021166875.1	XP_021022534.1	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following Bckdk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bckdk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMv04508	XM_021166875.1 Latest version!	Mus caroli branched chain keto acid dehydrogenase kinase (Bckdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bckdk ( XM_021166875.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021022534.1 Mus caroli branched chain keto acid dehydrogenase kinase (Bckdk), mRNA.

Price & Availability↑

CloneID	OMv04508
Clone ID Related Accession (Same CDS sequence)	XM_021166875.1
Accession Version	XM_021166875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACTGA CTTCAGTGCT CGGCAGCAGC CCTCGGAGCT GGTCTTCACT TTGGCCCCTC 
TTGGGGTCCT CACTGTCACT CCGGGCTCGC TCAACATCAG CCACCGATAC ACACCATGTA 
GAGCTGGCCA GGGAACGCTC CAAGACTGTC ACCTCCTTTT ACAACCAGTC AGCCATAGAT 
GTGGCAGCAG AGAAGCCCTC AGTTCGCCTC ACTCCCACCA TGATGCTCTA TTCCGGTCGC 
TCACAGGATG GCAGCCACCT TCTGAAAAGT GGCCGCTACT TGCAGCAAGA GTTACCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGT AGCCTTCCTT TCATCATTGG TTGCAACCCT 
ACCATACTGC ATGTGCACGA GCTATACATC CGAGCCTTCC AGAAACTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCAGA CGAGGCCCAG TACTGCCAGC TGGTGAGACA GCTGTTAGAT 
GACCACAAGG ACGTGGTGAC CCTGTTAGCT GAGGGTCTTC GTGAGAGCCG GAAACACATC 
CAGGATGAAA AGCTAGTCCG GTACTTCTTG GATAAAACAC TGACGTCAAG ACTTGGGATC 
CGAATGCTGG CTACTCACCA CTTGGCGCTA CATGAAGACA AGCCTGATTT TGTTGGCATC 
ATCTGTACTC GTCTGTCGCC CAAGAAGATT ATCGAGAAGT GGGTGGATTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCCCCT CGAGTCCGCA TCAACGGGCA TGTGGCTGCC 
CGGTTCCCCT TCATTCCCAT GCCGCTGGAC TATATCCTGC CTGAGCTGCT CAAGAACGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ATAATGATAT TGATCTCATC ATCAGGATCT CAGACCGGGG TGGAGGAATC 
GCTCATAAGG ACCTGGATCG GGTCATGGAC TACCACTTCA CCACAGCTGA GGCCAGCACC 
CAGGACCCCC GAATCAGCCC CCTCTTCGGC CACCTGGATA TGCACAGTGG TGGCCAGTCA 
GGACCTATGC ATGGCTTTGG CTTCGGGTTG CCCACGTCCA GGGCCTATGC GGAGTATCTT 
GGTGGCTCCC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CAGATGTCTA CCTACGGCTC 
CGCCACATTG ATGGTCGGGA GGAAAGCTTC AGAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021022534.1
CDS	274..1512
Translation	MILTSVLGSSPRSWSSLWPLLGSSLSLRARSTSATDTHHVELARERSKTVTSFYNQSAIDVAAEKPSVRLTPTMMLYSGRSQDGSHLLKSGRYLQQELPVRIAHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIQDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDIDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGGQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

Target ORF information:

RefSeq Version	XM_021166875.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli branched chain keto acid dehydrogenase kinase (Bckdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166875.1

ORF Insert Sequence:

ATGATACTGA CTTCAGTGCT CGGCAGCAGC CCTCGGAGCT GGTCTTCACT TTGGCCCCTC 
TTGGGGTCCT CACTGTCACT CCGGGCTCGC TCAACATCAG CCACCGATAC ACACCATGTA 
GAGCTGGCCA GGGAACGCTC CAAGACTGTC ACCTCCTTTT ACAACCAGTC AGCCATAGAT 
GTGGCAGCAG AGAAGCCCTC AGTTCGCCTC ACTCCCACCA TGATGCTCTA TTCCGGTCGC 
TCACAGGATG GCAGCCACCT TCTGAAAAGT GGCCGCTACT TGCAGCAAGA GTTACCAGTG 
AGGATTGCTC ACCGCATCAA GGGCTTCCGT AGCCTTCCTT TCATCATTGG TTGCAACCCT 
ACCATACTGC ATGTGCACGA GCTATACATC CGAGCCTTCC AGAAACTGAC AGACTTCCCT 
CCGATCAAGG ACCAGGCAGA CGAGGCCCAG TACTGCCAGC TGGTGAGACA GCTGTTAGAT 
GACCACAAGG ACGTGGTGAC CCTGTTAGCT GAGGGTCTTC GTGAGAGCCG GAAACACATC 
CAGGATGAAA AGCTAGTCCG GTACTTCTTG GATAAAACAC TGACGTCAAG ACTTGGGATC 
CGAATGCTGG CTACTCACCA CTTGGCGCTA CATGAAGACA AGCCTGATTT TGTTGGCATC 
ATCTGTACTC GTCTGTCGCC CAAGAAGATT ATCGAGAAGT GGGTGGATTT TGCCAGACGC 
CTGTGTGAGC ACAAGTATGG CAATGCCCCT CGAGTCCGCA TCAACGGGCA TGTGGCTGCC 
CGGTTCCCCT TCATTCCCAT GCCGCTGGAC TATATCCTGC CTGAGCTGCT CAAGAACGCC 
ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACTCCCTACA ATGTCCCAGA TGTGGTCATC 
ACCATCGCCA ATAATGATAT TGATCTCATC ATCAGGATCT CAGACCGGGG TGGAGGAATC 
GCTCATAAGG ACCTGGATCG GGTCATGGAC TACCACTTCA CCACAGCTGA GGCCAGCACC 
CAGGACCCCC GAATCAGCCC CCTCTTCGGC CACCTGGATA TGCACAGTGG TGGCCAGTCA 
GGACCTATGC ATGGCTTTGG CTTCGGGTTG CCCACGTCCA GGGCCTATGC GGAGTATCTT 
GGTGGCTCCC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CAGATGTCTA CCTACGGCTC 
CGCCACATTG ATGGTCGGGA GGAAAGCTTC AGAATCTGA