LOC105661921 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105661921
Entrez Gene ID	105661921
Full Name	periodic tryptophan protein 1 homolog
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012280672.1	XP_012136062.1	periodic tryptophan protein 1 homolog

Pathways from BioSystems
Gene Ontology

The following LOC105661921 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105661921 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe100596	XM_012280672.1 Latest version!	Megachile rotundata periodic tryptophan protein 1 homolog (LOC105661921), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105661921 ( XM_012280672.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136062.1 Megachile rotundata periodic tryptophan protein 1 homolog (LOC105661921), mRNA.

Price & Availability↑

CloneID	OMe100596
Clone ID Related Accession (Same CDS sequence)	XM_012280672.1
Accession Version	XM_012280672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	periodic tryptophan protein 1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797113.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTAA TACCGTGTGC AACTTGGGTG AGAAGAGGTG TTGCGTCTAC GAATCCTCAG 
AAGGTTGAAC TGACACCTAA AGAATTAGAA CAGATTATAA AACAAACACA GTCCGAATTA 
CAAGATGCTG AAAGTGATGA AGAACAGGAC AATGTTGTGG AACAAAAAAT GGACACAAAA 
TCTGAACTTG ATACATGCGA AACTGATGAA TATAACTTTG ACAAATATGA TGAAGAATCA 
AGTAATATAC ATTGTAATAT TAAAAATATT GCAAGTTTCG GAGAAGATGG TCAAGATCCC 
TTTGTGACAG GGGATGATGA TGATTCAGAA AAGGAAGATG ATGCTATAAA AAGTGATGAC 
AATCTTGTAT TAGTTGGACA TGTTGATGGA GATGCTAGTA TTTTAGAAGT ATTTGTTTAT 
AATGAAGCTG AAGGTTCATT TTATTGTCAT CATGACATAC TGTTACCATC GTTCCCACTG 
TGTATGGAAT GGCTCAATTT TGATCCCACA GATTCAAAAC CAAGTAATTT ATGTGCAATT 
GGTAATATGA CTCCTATAAT AGATGTATGG GATTTGGATT TAATAGACTG TTTAGAACCA 
GCTTATAAAC TTGGTCGCAA GGCAAATAAG AAAAAAAATC AAAAACGTGT TGGACACAAA 
GATGCTGTAT TAGATTTGGC ATGGAATGAA AATTATGTAC ATGTACTTGC TAGTGGATCA 
GCTGATCAAA CAGTTCTTTT GTGGGATTTA GAAAATGGAA CACCTGTCAA TAAATTTGAT 
TCTTTTGATG AAAAAGTTCA ATCATTACAA TGGCATCCAA CAGAAACACA TCAGTTATTA 
ACAGGATGTG CAGATAAGAT CGTGAGATTA TTTGACTGTA GATACGAATC ACTTATGAAA 
TATTGGGAAG CAGCAGGAGA AATAGAGAGG GTGTTATGGA ATCGTTTTGA TTCTAATTAT 
TTAATAGCAA GTACCGACAA TGGATACATA CACTATATTG ATATAAGAGA AGATAAACCA 
ATCTGGAGTA TACAAGCTCA TACAAAAGAA GTTACAGGCT TATCTCTTAG CTCGTCGTGT 
TGTGGTCTTC TTGTTACGTC GGCAAATGAC GGTATAATAA AGGTATGGGA CATTATTAGT 
AACACAGAAC CAGAACTTAT TTTTGAGAAA AAAAGTAATC TTGGTGCTCT ACTATGTCTA 
ACATCAAATC CGGATAATCC TTTTGTATTT GCTGCTGGGG GAGATAACAA GTCATGCAAT 
TTTAAAGTAT TTGACTTCTT GGATATTTCG GAAGTACGCG AAAGATTTCA AACTAGAATA 
AATGTACAAA ACGGAAATAA CAGTACTGCG ATAAATCGAC AAAATGACAC GGGAGAAATG 
GTTGATGTAA CTGAATATAT GGATTCAATG ATTCTTAACG CGCACGGATC TGAACATAAG 
AAATACAATA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136062.1
CDS	147..1601
Translation	MNVIPCATWVRRGVASTNPQKVELTPKELEQIIKQTQSELQDAESDEEQDNVVEQKMDTKSELDTCETDEYNFDKYDEESSNIHCNIKNIASFGEDGQDPFVTGDDDDSEKEDDAIKSDDNLVLVGHVDGDASILEVFVYNEAEGSFYCHHDILLPSFPLCMEWLNFDPTDSKPSNLCAIGNMTPIIDVWDLDLIDCLEPAYKLGRKANKKKNQKRVGHKDAVLDLAWNENYVHVLASGSADQTVLLWDLENGTPVNKFDSFDEKVQSLQWHPTETHQLLTGCADKIVRLFDCRYESLMKYWEAAGEIERVLWNRFDSNYLIASTDNGYIHYIDIREDKPIWSIQAHTKEVTGLSLSSSCCGLLVTSANDGIIKVWDIISNTEPELIFEKKSNLGALLCLTSNPDNPFVFAAGGDNKSCNFKVFDFLDISEVRERFQTRINVQNGNNSTAINRQNDTGEMVDVTEYMDSMILNAHGSEHKKYNK

Target ORF information:

RefSeq Version	XM_012280672.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata periodic tryptophan protein 1 homolog (LOC105661921), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012280672.1

ORF Insert Sequence:

ATGAATGTAA TACCGTGTGC AACTTGGGTG AGAAGAGGTG TTGCGTCTAC GAATCCTCAG 
AAGGTTGAAC TGACACCTAA AGAATTAGAA CAGATTATAA AACAAACACA GTCCGAATTA 
CAAGATGCTG AAAGTGATGA AGAACAGGAC AATGTTGTGG AACAAAAAAT GGACACAAAA 
TCTGAACTTG ATACATGCGA AACTGATGAA TATAACTTTG ACAAATATGA TGAAGAATCA 
AGTAATATAC ATTGTAATAT TAAAAATATT GCAAGTTTCG GAGAAGATGG TCAAGATCCC 
TTTGTGACAG GGGATGATGA TGATTCAGAA AAGGAAGATG ATGCTATAAA AAGTGATGAC 
AATCTTGTAT TAGTTGGACA TGTTGATGGA GATGCTAGTA TTTTAGAAGT ATTTGTTTAT 
AATGAAGCTG AAGGTTCATT TTATTGTCAT CATGACATAC TGTTACCATC GTTCCCACTG 
TGTATGGAAT GGCTCAATTT TGATCCCACA GATTCAAAAC CAAGTAATTT ATGTGCAATT 
GGTAATATGA CTCCTATAAT AGATGTATGG GATTTGGATT TAATAGACTG TTTAGAACCA 
GCTTATAAAC TTGGTCGCAA GGCAAATAAG AAAAAAAATC AAAAACGTGT TGGACACAAA 
GATGCTGTAT TAGATTTGGC ATGGAATGAA AATTATGTAC ATGTACTTGC TAGTGGATCA 
GCTGATCAAA CAGTTCTTTT GTGGGATTTA GAAAATGGAA CACCTGTCAA TAAATTTGAT 
TCTTTTGATG AAAAAGTTCA ATCATTACAA TGGCATCCAA CAGAAACACA TCAGTTATTA 
ACAGGATGTG CAGATAAGAT CGTGAGATTA TTTGACTGTA GATACGAATC ACTTATGAAA 
TATTGGGAAG CAGCAGGAGA AATAGAGAGG GTGTTATGGA ATCGTTTTGA TTCTAATTAT 
TTAATAGCAA GTACCGACAA TGGATACATA CACTATATTG ATATAAGAGA AGATAAACCA 
ATCTGGAGTA TACAAGCTCA TACAAAAGAA GTTACAGGCT TATCTCTTAG CTCGTCGTGT 
TGTGGTCTTC TTGTTACGTC GGCAAATGAC GGTATAATAA AGGTATGGGA CATTATTAGT 
AACACAGAAC CAGAACTTAT TTTTGAGAAA AAAAGTAATC TTGGTGCTCT ACTATGTCTA 
ACATCAAATC CGGATAATCC TTTTGTATTT GCTGCTGGGG GAGATAACAA GTCATGCAAT 
TTTAAAGTAT TTGACTTCTT GGATATTTCG GAAGTACGCG AAAGATTTCA AACTAGAATA 
AATGTACAAA ACGGAAATAA CAGTACTGCG ATAAATCGAC AAAATGACAC GGGAGAAATG 
GTTGATGTAA CTGAATATAT GGATTCAATG ATTCTTAACG CGCACGGATC TGAACATAAG 
AAATACAATA AATAA