LOC100883154 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100883154
Entrez Gene ID	100883154
Full Name	F-box only protein 9
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012293139.1	XP_012148529.1	F-box only protein 9

Pathways from BioSystems
Gene Ontology

The following LOC100883154 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100883154 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe111559	XM_012293139.1 Latest version!	Megachile rotundata F-box only protein 9 (LOC100883154), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100883154 ( XM_012293139.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012148529.1 Megachile rotundata F-box only protein 9 (LOC100883154), mRNA.

Price & Availability↑

CloneID	OMe111559
Clone ID Related Accession (Same CDS sequence)	XM_012293139.1
Accession Version	XM_012293139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	F-box only protein 9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797463.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCACT ACTGTGAATC TGGTGAATCT GACGATGGTG GAGATGACCA GGAAAGTTCT 
TCTTACTCGG GGACAAATAT CGAAGATGCT TTAACATCTT TCAGAGAGCA ATGGCAGCGG 
GAGTTAGAAA TATCTCCGAA ACGAGAAAGA TCAAAGACTC GTTCCACAAA ACTAGTTGAC 
TTTGATGTTT CCAATGGAGA AGAGTCCTTG GATACCATTG AAAATAAAGC AAAAAATCTG 
TTTTTGAAAG GAATTGAATA TGAAGAACGT AGGAAGTTTT ATGAAGCTAT TCAATTTTAC 
AAACGCGCTG TACTGTTAGT TCCTGATATT GAATTACGTT TATACGAATC GACCAAACTC 
AAATCAAATG ACGAGGTCGG TGAACAACTA GAGGACGACG TAAATAGTAT TGAAGATAAT 
ACTGAAAATC ATAATGAAGA AGAAGAAGAA GAAACTGATT TATTTGTTAA ATTGTGTAAA 
ATTGTAAATC ATAACAAATG CGTTTGTTTT CCTAAATTTC AGCAAGATGC AACTCATATT 
TCTGCTTTGC CTATGGAAAT AATGCTTTAT ATATTAAGAT GGGTTGTGTC TTCCGAATTA 
GATTTGAGAT CCCTTGAAAT GTTCTCCAGA GTGTGTCGTG GATTTTACAT ATCTGCAAGA 
GACACAGAGA TCTGGAGACT TGCCTGTATT AGAGTATGGG GTGTAAATTG CGGTACCTAT 
GCTCCCAAGT ATCGGTCATG GAGAGATATG TATTTACAAC GGCCTAGATT AAGATACAAT 
GGGTGTTATA TTAGTAAAAC CAGTTATATT CGCGATGGTG AAAACAGTTT CCAAGATCGA 
TTTTACAGAC CTTGGCATTT GGTGGAATAC TTCAGGTACC TGAGATTTTT TCCAGAAGGC 
AGAGTTTTAA TGCTAACCTC GACAGATGAA GCACAAAATT GTGTAAACTC TTTAAAAAAC 
CGTGCTCCAC GAAATTCATC GATTCTTGTT GGCCATTACA GGTTGCACGA TAATTATGTT 
AATTTAGTGC TCAAGAAACA AGAAACGAAA CCTGTGAATA CATACAGAAA GAAGAAAAGA 
GAACCTATAC ATGATAGTGG GGAACAAACT TTTCATCTTG AATTTGAAAT TCAGGACCAC 
CATAGACGGT TAAACTCACA ATTAAAATGG CTCAGTTATA CTATATTTAC AAAGTATAGA 
AACGGACACG AAGTAAAAAT GTGCTTGAAG GAGCCATCTG TCAGAGAATG GAGAGTGTCG 
GCAATCGGCG GACGATACCC GCCTCTTAAA TTTAGTAGAG TTAAGAGTTA TACTCAAGAA 
AGCGAAGCTC CTTTGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012148529.1
CDS	273..1613
Translation	MSHYCESGESDDGGDDQESSSYSGTNIEDALTSFREQWQRELEISPKRERSKTRSTKLVDFDVSNGEESLDTIENKAKNLFLKGIEYEERRKFYEAIQFYKRAVLLVPDIELRLYESTKLKSNDEVGEQLEDDVNSIEDNTENHNEEEEEETDLFVKLCKIVNHNKCVCFPKFQQDATHISALPMEIMLYILRWVVSSELDLRSLEMFSRVCRGFYISARDTEIWRLACIRVWGVNCGTYAPKYRSWRDMYLQRPRLRYNGCYISKTSYIRDGENSFQDRFYRPWHLVEYFRYLRFFPEGRVLMLTSTDEAQNCVNSLKNRAPRNSSILVGHYRLHDNYVNLVLKKQETKPVNTYRKKKREPIHDSGEQTFHLEFEIQDHHRRLNSQLKWLSYTIFTKYRNGHEVKMCLKEPSVREWRVSAIGGRYPPLKFSRVKSYTQESEAPLQ

Target ORF information:

RefSeq Version	XM_012293139.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata F-box only protein 9 (LOC100883154), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012293139.1

ORF Insert Sequence:

ATGAGTCACT ACTGTGAATC TGGTGAATCT GACGATGGTG GAGATGACCA GGAAAGTTCT 
TCTTACTCGG GGACAAATAT CGAAGATGCT TTAACATCTT TCAGAGAGCA ATGGCAGCGG 
GAGTTAGAAA TATCTCCGAA ACGAGAAAGA TCAAAGACTC GTTCCACAAA ACTAGTTGAC 
TTTGATGTTT CCAATGGAGA AGAGTCCTTG GATACCATTG AAAATAAAGC AAAAAATCTG 
TTTTTGAAAG GAATTGAATA TGAAGAACGT AGGAAGTTTT ATGAAGCTAT TCAATTTTAC 
AAACGCGCTG TACTGTTAGT TCCTGATATT GAATTACGTT TATACGAATC GACCAAACTC 
AAATCAAATG ACGAGGTCGG TGAACAACTA GAGGACGACG TAAATAGTAT TGAAGATAAT 
ACTGAAAATC ATAATGAAGA AGAAGAAGAA GAAACTGATT TATTTGTTAA ATTGTGTAAA 
ATTGTAAATC ATAACAAATG CGTTTGTTTT CCTAAATTTC AGCAAGATGC AACTCATATT 
TCTGCTTTGC CTATGGAAAT AATGCTTTAT ATATTAAGAT GGGTTGTGTC TTCCGAATTA 
GATTTGAGAT CCCTTGAAAT GTTCTCCAGA GTGTGTCGTG GATTTTACAT ATCTGCAAGA 
GACACAGAGA TCTGGAGACT TGCCTGTATT AGAGTATGGG GTGTAAATTG CGGTACCTAT 
GCTCCCAAGT ATCGGTCATG GAGAGATATG TATTTACAAC GGCCTAGATT AAGATACAAT 
GGGTGTTATA TTAGTAAAAC CAGTTATATT CGCGATGGTG AAAACAGTTT CCAAGATCGA 
TTTTACAGAC CTTGGCATTT GGTGGAATAC TTCAGGTACC TGAGATTTTT TCCAGAAGGC 
AGAGTTTTAA TGCTAACCTC GACAGATGAA GCACAAAATT GTGTAAACTC TTTAAAAAAC 
CGTGCTCCAC GAAATTCATC GATTCTTGTT GGCCATTACA GGTTGCACGA TAATTATGTT 
AATTTAGTGC TCAAGAAACA AGAAACGAAA CCTGTGAATA CATACAGAAA GAAGAAAAGA 
GAACCTATAC ATGATAGTGG GGAACAAACT TTTCATCTTG AATTTGAAAT TCAGGACCAC 
CATAGACGGT TAAACTCACA ATTAAAATGG CTCAGTTATA CTATATTTAC AAAGTATAGA 
AACGGACACG AAGTAAAAAT GTGCTTGAAG GAGCCATCTG TCAGAGAATG GAGAGTGTCG 
GCAATCGGCG GACGATACCC GCCTCTTAAA TTTAGTAGAG TTAAGAGTTA TACTCAAGAA 
AGCGAAGCTC CTTTGCAATA A