LOC100882911 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100882911
Entrez Gene ID	100882911
Full Name	pro-interleukin-16-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012281125.1	XP_012136515.1	pro-interleukin-16-like isoform X3
XM_012281123.1	XP_012136513.1	pro-interleukin-16-like isoform X3
XM_012281126.1	XP_012136516.1	pro-interleukin-16-like isoform X3
XM_012281124.1	XP_012136514.1	pro-interleukin-16-like isoform X3
XM_012281121.1	XP_012136511.1	pro-interleukin-16-like isoform X1
XM_012281129.1	XP_012136519.1	pro-interleukin-16-like isoform X3
XM_012281122.1	XP_012136512.1	pro-interleukin-16-like isoform X2
XM_012281128.1	XP_012136518.1	pro-interleukin-16-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC100882911 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100882911 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe101006	XM_012281126.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101006	XM_012281129.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101006	XM_012281123.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101006	XM_012281124.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101006	XM_012281128.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101006	XM_012281125.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101004	XM_012281121.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OMe101005	XM_012281122.1 Latest version!	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100882911 ( XM_012281125.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136515.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMe101006
Clone ID Related Accession (Same CDS sequence)	XM_012281125.1 , XM_012281123.1 , XM_012281126.1 , XM_012281124.1 , XM_012281129.1 , XM_012281128.1
Accession Version	XM_012281125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136515.1
CDS	117..4382
Translation	MDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281125.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281125.1

ORF Insert Sequence:

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882911 ( XM_012281123.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136513.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe101006
Clone ID Related Accession (Same CDS sequence)	XM_012281125.1 , XM_012281123.1 , XM_012281126.1 , XM_012281124.1 , XM_012281129.1 , XM_012281128.1
Accession Version	XM_012281123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136513.1
CDS	2008..6273
Translation	MDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281123.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281123.1

ORF Insert Sequence:

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882911 ( XM_012281126.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136516.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X6, mRNA.

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CloneID	OMe101006
Clone ID Related Accession (Same CDS sequence)	XM_012281125.1 , XM_012281123.1 , XM_012281126.1 , XM_012281124.1 , XM_012281129.1 , XM_012281128.1
Accession Version	XM_012281126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136516.1
CDS	4018..8283
Translation	MDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281126.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281126.1

ORF Insert Sequence:

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882911 ( XM_012281124.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136514.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X4, mRNA.

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CloneID	OMe101006
Clone ID Related Accession (Same CDS sequence)	XM_012281125.1 , XM_012281123.1 , XM_012281126.1 , XM_012281124.1 , XM_012281129.1 , XM_012281128.1
Accession Version	XM_012281124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136514.1
CDS	188..4453
Translation	MDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281124.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281124.1

ORF Insert Sequence:

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882911 ( XM_012281129.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136519.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMe101006
Clone ID Related Accession (Same CDS sequence)	XM_012281125.1 , XM_012281123.1 , XM_012281126.1 , XM_012281124.1 , XM_012281129.1 , XM_012281128.1
Accession Version	XM_012281129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136519.1
CDS	192..4457
Translation	MDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281129.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281129.1

ORF Insert Sequence:

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882911 ( XM_012281128.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136518.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMe101006
Clone ID Related Accession (Same CDS sequence)	XM_012281125.1 , XM_012281123.1 , XM_012281126.1 , XM_012281124.1 , XM_012281129.1 , XM_012281128.1
Accession Version	XM_012281128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136518.1
CDS	1933..6198
Translation	MDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281128.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281128.1

ORF Insert Sequence:

ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882911 ( XM_012281121.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136511.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X1, mRNA.

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CloneID	OMe101004
Clone ID Related Accession (Same CDS sequence)	XM_012281121.1
Accession Version	XM_012281121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACT ACAACGTTTA CGAGGACGAG GGACTAAGAA CGAATAAAGT ATTGGAGATA 
ATCGAAATAA GGAAGGCGAA GGCTTTCTCG CCGACGATCG CTAATTCTAA GGATGACAAG 
AGCAAAGGTA AGGGCGCCGA GAACGACCGA GTTAACGGCG ATAGCGAGAA CGTTCGAACG 
TATGAGAGAA TGAAACCGGA ATCGAGAAGC AGAACCATCT CGACGGAAGT CGAAGAAGAC 
TCGGAAGAGG AACACGAGAT GAGCACGAAA TTTAACGAGC CTATTGATTT TCAATGCGAG 
ACGACGGATC GTTCGGACGA TACGGTTTCG TCGTCGATCG ATGGACGATC GATCCAAGAT 
TCTTATCGTT CGTGCAACGA TATCGGTCCG CGTGAACCGA CCGTGAACGG AACAGGGAAC 
GAAAAGGGAA GCGTCGAAGA TGACGATACG AGCGAGGAAA GCGACGACGG CGATTACTAT 
TGGCAAAGTA ATTTGGCCAC CATAGGCGAG GAAGAGGAAA CGAATTCTCT CGAATACACG 
AACGCGAACA AAGAGTCGAG CGACAGCGAA AGTAACGCGA CGGAGAAATC GGAACGGCAG 
GAATCGCCGA GCAAGAAAGA CGCAAGTGAG CGGAATGACG AGGTAGACAG TACTGTTCGA 
AACGCGAGAT TCCCCGAGGC CATCGGCGCA GATCGAAAAG CAGACACGGT AAATGGTAGA 
ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136511.1
CDS	200..5185
Translation	MENYNVYEDEGLRTNKVLEIIEIRKAKAFSPTIANSKDDKSKGKGAENDRVNGDSENVRTYERMKPESRSRTISTEVEEDSEEEHEMSTKFNEPIDFQCETTDRSDDTVSSSIDGRSIQDSYRSCNDIGPREPTVNGTGNEKGSVEDDDTSEESDDGDYYWQSNLATIGEEEETNSLEYTNANKESSDSESNATEKSERQESPSKKDASERNDEVDSTVRNARFPEAIGADRKADTVNGRMDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281121.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281121.1

ORF Insert Sequence:

ATGGAGAACT ACAACGTTTA CGAGGACGAG GGACTAAGAA CGAATAAAGT ATTGGAGATA 
ATCGAAATAA GGAAGGCGAA GGCTTTCTCG CCGACGATCG CTAATTCTAA GGATGACAAG 
AGCAAAGGTA AGGGCGCCGA GAACGACCGA GTTAACGGCG ATAGCGAGAA CGTTCGAACG 
TATGAGAGAA TGAAACCGGA ATCGAGAAGC AGAACCATCT CGACGGAAGT CGAAGAAGAC 
TCGGAAGAGG AACACGAGAT GAGCACGAAA TTTAACGAGC CTATTGATTT TCAATGCGAG 
ACGACGGATC GTTCGGACGA TACGGTTTCG TCGTCGATCG ATGGACGATC GATCCAAGAT 
TCTTATCGTT CGTGCAACGA TATCGGTCCG CGTGAACCGA CCGTGAACGG AACAGGGAAC 
GAAAAGGGAA GCGTCGAAGA TGACGATACG AGCGAGGAAA GCGACGACGG CGATTACTAT 
TGGCAAAGTA ATTTGGCCAC CATAGGCGAG GAAGAGGAAA CGAATTCTCT CGAATACACG 
AACGCGAACA AAGAGTCGAG CGACAGCGAA AGTAACGCGA CGGAGAAATC GGAACGGCAG 
GAATCGCCGA GCAAGAAAGA CGCAAGTGAG CGGAATGACG AGGTAGACAG TACTGTTCGA 
AACGCGAGAT TCCCCGAGGC CATCGGCGCA GATCGAAAAG CAGACACGGT AAATGGTAGA 
ATGGACAACT GTGGAGGCGG TCAACGCCTG CCCCCGGACG GGGACGAGTT TCCAGCAGCC 
TATCAGGAGT TTGGCGGCAG TAACCAGCAG TACCGATTCG TGACGGCGAC GGCTACTGCC 
CCGAGGACGT CGGTGATGAC GACGAACGGC AGACTCGCCT GTAACGAGGA CGAGTCGTTC 
CTGGCTAACG AGAGTTTTAA ACAGCGCGAG AACAACGCGG TGAACGCGAC GAGCACGATC 
CTGGTCGACG ACAGAGTCGA AAACGTTTGT CAGGAGGTGG GCAAGATCGG TTTACCGAGC 
ATCGAGATAA ACGGCAAGAC GAGTATCGAG AACGTTTGCC AGGACGCGAG CTCGTTGACA 
CGAGAGTCTC GCGCGGACGA CGCCAAACCG TTTGTCTCGG AAGATTTCGT AAAAAAGACC 
GCGTCCATGA CCAACTATTC CAGCTACGGA AACCAAAGCA ACGGTAACGT GATCGTCTCG 
GAAAAGAAGA TAGAAATCAC TGGATATTAT CACAAAGACG TGCCAGTTAT CGAACAGTTT 
CGGGACGAGA AGGATGCTTT GGATACGCCT CCGCCGCTTC CGCTCACAGG TCCGCCTAAA 
TTGGAGCCCG TCAGTCCATA CGCGAGAAAC GTCTCGGTTC TGGAATCCTC GCCAAGGAAG 
TTCTCGTTGA ACGGCGAGCT CTGGAGGAAG GACGACAAGT CCGAGAAATC CGTCAGGGAC 
AAGATCGCGA TGTTCTCGTC CCAGAGCAGC CTGGATGGAC CTCTGTTTCC GAACACGATA 
ACCGTCGCTA CCGCGACGAG TAACGCCAGG AAGCTGTCCA AGTACAAATC CTCCGAGGAC 
GTGTGCAACG ACGAGAAGAG CTCTAGCCAG AAGGAGAGAG TCTCGTTTTT CGCGGATCGG 
ACGCAGAGCT CTTTCGATTT GACGGACTCG GGCCGAAACG CGAATCAGGA ATCGGTGAAA 
AAGTACAGAC AAAGATCGCC CAGTATTTCT CAGAGTCCGT CGTTTGCTAC GCCGACTATT 
CACGCATCGA AGACGTTTCC GATCGTTGCT CAGAAGCCGA CCGCGTCGAC GCCAACGTTG 
ATAAGCTCCT TCGAAACGTC ACCGACATCC AAATCGCCGG TGAAGAGCTA CTGCTCTTCC 
GCCGGCAGTC AAAACTCTGC GATAACCCCA TTGTCGAAGC CCGTTACGAG CACGGCGTTG 
TCTCGCGCTA CCAGCTTCTC CGGATCGAGC CCTTACGGTC AAGATCGAAC CTTGGCGACG 
AACGAATTGT CGAGCAGCTC TCAGATCACC AGAACGAATT CTTTGGCGTC GACGTTTAAG 
AAACCCAACG AGGATATCAG GAGAAACAGC CTGAATCAAC TGATAGAACA GAGAAGGCGA 
TCGATATCGA AATTGAGGGG ACTGGTTATT CCGGAAAAAG AGAGCATACC TATCGATACG 
CCTATCGTCG ATTTGCCTGA AATTAAATCT CGAGACTCCA TACTACTGCA TCAGCTATCG 
AAGGCCAATA TCCAAGACAA GTGGGGCTCC CAAAGTTCGT TGGCAAGCAA CGCGAGCACG 
GCCAGTATGC CCGTGAAAAC TACCTCCTTC AAGATGCCGG CTCCTCAAAT ACACTCGAAA 
TACTCTCCAG CTTTCAAAAG AAAGAGCTTG ACAGTGTACG GTTCGTCCTC GACGAATTGT 
TCGTTGTACG GTATCGCCGC GGTGAAAAGT AGTCCGATAG CGACGACTAC CGCGACATCC 
ACGCCGACGC TCTCCGAACC CCCAAAGAGT CTGGAGAGCA TCTGCAGTCC GACGAGGAGC 
GATTACAGTT TCGAGTTCGC GTCGACGGCC AGTTCGCCCG AAGGTTCGCG CAACAGACCG 
CTCAGAACCG TTCAGAGAAA GACGCGAATG GATTACGACG ATTCCGACAA CGATTCCGCC 
GTAAGCAGTT CGCAAAGCAG CATTTCGCGT GGCTTTAGCC CGCCTATGTC CCCGGTACCT 
TCGGAACGCT CCATCGTTTC CTCCAAACGA TCCTACGTCT CCACGGAGAA CAACTATCCG 
CGACGAGCTC TGATCGTCGA CAGCCCAGCT AGAAGCTACG GCAGAGAATC CAACGTTCTC 
GAGGAGAGAA CGTTCGCGCC TGACCGATCG TCGTCCAGTA GCAGCGGTTC CGAGCACAGA 
TCACCGTTGC CGATCACCGA ATCGAACGCG AGAAACTCTC AAATACCTCA GAGACAGTTA 
AAGCGATCCA ACAGTACCGA GACCAACTGC AGCACGTCGT CGACTCTCAC TTCCGGTTCT 
CAAGCGAGCG CGGAGTCTCT GTCGAGAAGA GTACTGAAGC CTCAGAGCGT GGAAGCCATC 
AATCGGAAGA ACATTTTGGC GAGCGCCAGA TGCCGAAGCG GCAGGGACCT GAACGGATCT 
CCGTTGATTC AACGAAAGTT CCCGGAGGAC GAGTGTCGAG CGACCATCGT CGAAAACGGT 
GACGATAACT ACCAGAGGAA TCGTTCGAAG GACGCGACCG CGAAAGACGT AAAGATCGCG 
TACATCGAGG TAACGGACTC GTTCGGGGAA GCCGAGTCTT TCCAGAAGGA GGCGAACGAG 
TCGAAATTGC CCTCGAAGAG AAGCGTCGTA TCGTCCACCG TGCGGTCGTC CAAATTCGAG 
ACGATAGAAC CGTCCAACGA CTTAAAAATG TGGGTTCGAA CGGAAGCGAA AGCGTTGGCT 
AGATCGGCTG AAGAGAGGAA CAACAAGATC GAGAAAAGAT CTCAAGAGGT CGATATCGTT 
CCTGCAAATA ACCCTTCGAA GAACAGCAGA TCGAGCAGCA TCGAGGAACA ACGGCTTGAT 
TCATCGTCGA CGAACAGGAA CGCGAGAAAC GAAAACTCCG GATCGCCGGA AGCTTCGAAA 
CAACGATGCG GTTCGAACGT ATTAACCTCG AAGAATCTGT TGGACGATCA GAACGATCTT 
CTGACGATTC TCACGACCAA AAGTAGATCC AGATCGGCGA TACACGTGGA CGAGGGAAGT 
TTCGGACGAT CGAAGAGCCA AATGAGTTTG GAGGATATTC TGAGCGCGAA AACGGATCCA 
AAATTACCGC GCACACCGAC CAATGATTCC AAGATCGAGA AGAACACCGT ACTTCCGGCT 
GTTCAGACCA GATCTCTGTG GGAGTCGAGG GACAATCGAC GCGTCAGGAG GAGCTCGTCG 
ACCTCGAACG AACTATGGAA CGAGGAGAAG CCGCTTTCGA AGATACCAAC GTTGAACAGG 
TCGAGACTAA ACGATAGCAG CGGCGACGAA ACGACGGAAA TCGAAAGATC AAAGTCGTTG 
CTGTCGAAGA TCCCAACCGC GAGGAGCCTT GCTCGAAGAA GCGCCAGCGT CACGGACATG 
AAGAAAGCCC TAGAGAAGAC CGAGGTGAAC TGTACGACTG GTACGCAGCA GACTGCTCAT 
AATCGATTTC CGAGTCTGGA CAGCAGCGTA GACGAGAACG TGTCACCTGT TGGAACGGAC 
GTAGACACGG ATAGGTGCTG CAGCGAACAA TTCGGTAGCA TCAGTTCTTT GGCCAGTAGT 
ACCAGCCTTA TCTCGCAACA GGAACTAGCT CAGTTAGTAG AGGAAGCGAG TCTGGAAGAG 
GTTCGAGGTG CTCATGACGT GATCGTGGTA TTACTGCACA AAGAGAATCA TGCCGGTAGC 
GTTGGAATCA CGTTGGCTGG CGGAAGCGAT TGCGAGATCA AAGAAATTAC GGTTCATCGA 
GTATTGGCGC ATTCTATAGC CGACAAGGAC GGACGTGTGC AGAGGGGAGA CAGAATTTTA 
AGCATAAACG GAAGAAGCAC GCAAGGTCTC ACGCACAGGG AGAGCATTGC GGTGCTTAAA 
CAACCTAGGT CGGAAGTCGT CTTGGTAGTT TCTCGAGCGA GAACCGAGGA AGGTTGTAAA 
TTGAGATCAA GGGCAGCGAG CGTCGAAACT ATTATCGAAG GATTCGAGAC GAACGATGTC 
ACGGAGGATA CTGCGTGGGG ACCGCCTTCG GTTGTAGCCG TGTACAAAGA TGGAGCTGGT 
TTGGGATTCA GTCTGGAGGG AGGCAGAGAC AGCCCTCTTG GAAACAGACC GTTGTTGATC 
AAGAAAATAT TTACCGGAGG TGCTGCCGAA AAAACGGGTG CCTTAAAGGC AGGAGATCAA 
CTATTGGAAG TGAACGGATA CGATGTTACG CGTATGTCGA GGATCGAAGC GTGGTCCCTT 
ATGAAGAAAC TACACGACGG CGAAGTGAAC CTCCTAGTCA GACACCCTGC CACTAAATCC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882911 ( XM_012281122.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136512.1 Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X2, mRNA.

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CloneID	OMe101005
Clone ID Related Accession (Same CDS sequence)	XM_012281122.1
Accession Version	XM_012281122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pro-interleukin-16-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACT ACAACGTTTA CGAGGACGAG GGACTAAGAA CGAATAAAGT ATTGGAGATA 
ATCGAAATAA GGAAGGCGAA GGCTTTCTCG CCGACGATCG CTAATTCTAA GGATGACAAG 
AGCAAAGGTA AGGGCGCCGA GAACGACCGA GTTAACGGCG ATAGCGAGAA CGTTCGAACG 
TATGAGAGAA TGAAACCGGA ATCGAGAAGC AGAACCATCT CGACGGAAGT CGAAGAAGAC 
TCGGAAGAGG AACACGAGAT GAGCACGAAA TTTAACGAGC CTATTGATTT TCAATGCGAG 
ACGACGGATC GTTCGGACGA TACGGTTTCG TCGTCGATCG ATGGACGATC GATCCAAGAT 
TCTTATCGTT CGTGCAACGA TATCGGTCCG CGTGAACCGA CCGTGAACGG AACAGGGAAC 
GAAAAGGGAA GCGTCGAAGA TGACGATACG AGCGAGGAAA GCGACGACGG CGATTACTAT 
TGGCAAAGTA ATTTGGCCAC CATAGGCGAG GAAGAGGAAA CGAATTCTCT CGAATACACG 
AACGCGAACA AAGAGTCGAG CGACAGCGAA AGTAACGCGA CGGAGAAATC GGAACGGCAG 
GAATCGCCGA GCAAGAAAGA CGCAAGTGAC ACGGTAAATG GTAGAATGGA CAACTGTGGA 
GGCGGTCAAC GCCTGCCCCC GGACGGGGAC GAGTTTCCAG CAGCCTATCA GGAGTTTGGC 
GGCAGTAACC AGCAGTACCG ATTCGTGACG GCGACGGCTA CTGCCCCGAG GACGTCGGTG 
ATGACGACGA ACGGCAGACT CGCCTGTAAC GAGGACGAGT CGTTCCTGGC TAACGAGAGT 
TTTAAACAGC GCGAGAACAA CGCGGTGAAC GCGACGAGCA CGATCCTGGT CGACGACAGA 
GTCGAAAACG TTTGTCAGGA GGTGGGCAAG ATCGGTTTAC CGAGCATCGA GATAAACGGC 
AAGACGAGTA TCGAGAACGT TTGCCAGGAC GCGAGCTCGT TGACACGAGA GTCTCGCGCG 
GACGACGCCA AACCGTTTGT CTCGGAAGAT TTCGTAAAAA AGACCGCGTC CATGACCAAC 
TATTCCAGCT ACGGAAACCA AAGCAACGGT AACGTGATCG TCTCGGAAAA GAAGATAGAA 
ATCACTGGAT ATTATCACAA AGACGTGCCA GTTATCGAAC AGTTTCGGGA CGAGAAGGAT 
GCTTTGGATA CGCCTCCGCC GCTTCCGCTC ACAGGTCCGC CTAAATTGGA GCCCGTCAGT 
CCATACGCGA GAAACGTCTC GGTTCTGGAA TCCTCGCCAA GGAAGTTCTC GTTGAACGGC 
GAGCTCTGGA GGAAGGACGA CAAGTCCGAG AAATCCGTCA GGGACAAGAT CGCGATGTTC 
TCGTCCCAGA GCAGCCTGGA TGGACCTCTG TTTCCGAACA CGATAACCGT CGCTACCGCG 
ACGAGTAACG CCAGGAAGCT GTCCAAGTAC AAATCCTCCG AGGACGTGTG CAACGACGAG 
AAGAGCTCTA GCCAGAAGGA GAGAGTCTCG TTTTTCGCGG ATCGGACGCA GAGCTCTTTC 
GATTTGACGG ACTCGGGCCG AAACGCGAAT CAGGAATCGG TGAAAAAGTA CAGACAAAGA 
TCGCCCAGTA TTTCTCAGAG TCCGTCGTTT GCTACGCCGA CTATTCACGC ATCGAAGACG 
TTTCCGATCG TTGCTCAGAA GCCGACCGCG TCGACGCCAA CGTTGATAAG CTCCTTCGAA 
ACGTCACCGA CATCCAAATC GCCGGTGAAG AGCTACTGCT CTTCCGCCGG CAGTCAAAAC 
TCTGCGATAA CCCCATTGTC GAAGCCCGTT ACGAGCACGG CGTTGTCTCG CGCTACCAGC 
TTCTCCGGAT CGAGCCCTTA CGGTCAAGAT CGAACCTTGG CGACGAACGA ATTGTCGAGC 
AGCTCTCAGA TCACCAGAAC GAATTCTTTG GCGTCGACGT TTAAGAAACC CAACGAGGAT 
ATCAGGAGAA ACAGCCTGAA TCAACTGATA GAACAGAGAA GGCGATCGAT ATCGAAATTG 
AGGGGACTGG TTATTCCGGA AAAAGAGAGC ATACCTATCG ATACGCCTAT CGTCGATTTG 
CCTGAAATTA AATCTCGAGA CTCCATACTA CTGCATCAGC TATCGAAGGC CAATATCCAA 
GACAAGTGGG GCTCCCAAAG TTCGTTGGCA AGCAACGCGA GCACGGCCAG TATGCCCGTG 
AAAACTACCT CCTTCAAGAT GCCGGCTCCT CAAATACACT CGAAATACTC TCCAGCTTTC 
AAAAGAAAGA GCTTGACAGT GTACGGTTCG TCCTCGACGA ATTGTTCGTT GTACGGTATC 
GCCGCGGTGA AAAGTAGTCC GATAGCGACG ACTACCGCGA CATCCACGCC GACGCTCTCC 
GAACCCCCAA AGAGTCTGGA GAGCATCTGC AGTCCGACGA GGAGCGATTA CAGTTTCGAG 
TTCGCGTCGA CGGCCAGTTC GCCCGAAGGT TCGCGCAACA GACCGCTCAG AACCGTTCAG 
AGAAAGACGC GAATGGATTA CGACGATTCC GACAACGATT CCGCCGTAAG CAGTTCGCAA 
AGCAGCATTT CGCGTGGCTT TAGCCCGCCT ATGTCCCCGG TACCTTCGGA ACGCTCCATC 
GTTTCCTCCA AACGATCCTA CGTCTCCACG GAGAACAACT ATCCGCGACG AGCTCTGATC 
GTCGACAGCC CAGCTAGAAG CTACGGCAGA GAATCCAACG TTCTCGAGGA GAGAACGTTC 
GCGCCTGACC GATCGTCGTC CAGTAGCAGC GGTTCCGAGC ACAGATCACC GTTGCCGATC 
ACCGAATCGA ACGCGAGAAA CTCTCAAATA CCTCAGAGAC AGTTAAAGCG ATCCAACAGT 
ACCGAGACCA ACTGCAGCAC GTCGTCGACT CTCACTTCCG GTTCTCAAGC GAGCGCGGAG 
TCTCTGTCGA GAAGAGTACT GAAGCCTCAG AGCGTGGAAG CCATCAATCG GAAGAACATT 
TTGGCGAGCG CCAGATGCCG AAGCGGCAGG GACCTGAACG GATCTCCGTT GATTCAACGA 
AAGTTCCCGG AGGACGAGTG TCGAGCGACC ATCGTCGAAA ACGGTGACGA TAACTACCAG 
AGGAATCGTT CGAAGGACGC GACCGCGAAA GACGTAAAGA TCGCGTACAT CGAGGTAACG 
GACTCGTTCG GGGAAGCCGA GTCTTTCCAG AAGGAGGCGA ACGAGTCGAA ATTGCCCTCG 
AAGAGAAGCG TCGTATCGTC CACCGTGCGG TCGTCCAAAT TCGAGACGAT AGAACCGTCC 
AACGACTTAA AAATGTGGGT TCGAACGGAA GCGAAAGCGT TGGCTAGATC GGCTGAAGAG 
AGGAACAACA AGATCGAGAA AAGATCTCAA GAGGTCGATA TCGTTCCTGC AAATAACCCT 
TCGAAGAACA GCAGATCGAG CAGCATCGAG GAACAACGGC TTGATTCATC GTCGACGAAC 
AGGAACGCGA GAAACGAAAA CTCCGGATCG CCGGAAGCTT CGAAACAACG ATGCGGTTCG 
AACGTATTAA CCTCGAAGAA TCTGTTGGAC GATCAGAACG ATCTTCTGAC GATTCTCACG 
ACCAAAAGTA GATCCAGATC GGCGATACAC GTGGACGAGG GAAGTTTCGG ACGATCGAAG 
AGCCAAATGA GTTTGGAGGA TATTCTGAGC GCGAAAACGG ATCCAAAATT ACCGCGCACA 
CCGACCAATG ATTCCAAGAT CGAGAAGAAC ACCGTACTTC CGGCTGTTCA GACCAGATCT 
CTGTGGGAGT CGAGGGACAA TCGACGCGTC AGGAGGAGCT CGTCGACCTC GAACGAACTA 
TGGAACGAGG AGAAGCCGCT TTCGAAGATA CCAACGTTGA ACAGGTCGAG ACTAAACGAT 
AGCAGCGGCG ACGAAACGAC GGAAATCGAA AGATCAAAGT CGTTGCTGTC GAAGATCCCA 
ACCGCGAGGA GCCTTGCTCG AAGAAGCGCC AGCGTCACGG ACATGAAGAA AGCCCTAGAG 
AAGACCGAGG TGAACTGTAC GACTGGTACG CAGCAGACTG CTCATAATCG ATTTCCGAGT 
CTGGACAGCA GCGTAGACGA GAACGTGTCA CCTGTTGGAA CGGACGTAGA CACGGATAGG 
TGCTGCAGCG AACAATTCGG TAGCATCAGT TCTTTGGCCA GTAGTACCAG CCTTATCTCG 
CAACAGGAAC TAGCTCAGTT AGTAGAGGAA GCGAGTCTGG AAGAGGTTCG AGGTGCTCAT 
GACGTGATCG TGGTATTACT GCACAAAGAG AATCATGCCG GTAGCGTTGG AATCACGTTG 
GCTGGCGGAA GCGATTGCGA GATCAAAGAA ATTACGGTTC ATCGAGTATT GGCGCATTCT 
ATAGCCGACA AGGACGGACG TGTGCAGAGG GGAGACAGAA TTTTAAGCAT AAACGGAAGA 
AGCACGCAAG GTCTCACGCA CAGGGAGAGC ATTGCGGTGC TTAAACAACC TAGGTCGGAA 
GTCGTCTTGG TAGTTTCTCG AGCGAGAACC GAGGAAGGTT GTAAATTGAG ATCAAGGGCA 
GCGAGCGTCG AAACTATTAT CGAAGGATTC GAGACGAACG ATGTCACGGA GGATACTGCG 
TGGGGACCGC CTTCGGTTGT AGCCGTGTAC AAAGATGGAG CTGGTTTGGG ATTCAGTCTG 
GAGGGAGGCA GAGACAGCCC TCTTGGAAAC AGACCGTTGT TGATCAAGAA AATATTTACC 
GGAGGTGCTG CCGAAAAAAC GGGTGCCTTA AAGGCAGGAG ATCAACTATT GGAAGTGAAC 
GGATACGATG TTACGCGTAT GTCGAGGATC GAAGCGTGGT CCCTTATGAA GAAACTACAC 
GACGGCGAAG TGAACCTCCT AGTCAGACAC CCTGCCACTA AATCCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136512.1
CDS	200..5110
Translation	MENYNVYEDEGLRTNKVLEIIEIRKAKAFSPTIANSKDDKSKGKGAENDRVNGDSENVRTYERMKPESRSRTISTEVEEDSEEEHEMSTKFNEPIDFQCETTDRSDDTVSSSIDGRSIQDSYRSCNDIGPREPTVNGTGNEKGSVEDDDTSEESDDGDYYWQSNLATIGEEEETNSLEYTNANKESSDSESNATEKSERQESPSKKDASDTVNGRMDNCGGGQRLPPDGDEFPAAYQEFGGSNQQYRFVTATATAPRTSVMTTNGRLACNEDESFLANESFKQRENNAVNATSTILVDDRVENVCQEVGKIGLPSIEINGKTSIENVCQDASSLTRESRADDAKPFVSEDFVKKTASMTNYSSYGNQSNGNVIVSEKKIEITGYYHKDVPVIEQFRDEKDALDTPPPLPLTGPPKLEPVSPYARNVSVLESSPRKFSLNGELWRKDDKSEKSVRDKIAMFSSQSSLDGPLFPNTITVATATSNARKLSKYKSSEDVCNDEKSSSQKERVSFFADRTQSSFDLTDSGRNANQESVKKYRQRSPSISQSPSFATPTIHASKTFPIVAQKPTASTPTLISSFETSPTSKSPVKSYCSSAGSQNSAITPLSKPVTSTALSRATSFSGSSPYGQDRTLATNELSSSSQITRTNSLASTFKKPNEDIRRNSLNQLIEQRRRSISKLRGLVIPEKESIPIDTPIVDLPEIKSRDSILLHQLSKANIQDKWGSQSSLASNASTASMPVKTTSFKMPAPQIHSKYSPAFKRKSLTVYGSSSTNCSLYGIAAVKSSPIATTTATSTPTLSEPPKSLESICSPTRSDYSFEFASTASSPEGSRNRPLRTVQRKTRMDYDDSDNDSAVSSSQSSISRGFSPPMSPVPSERSIVSSKRSYVSTENNYPRRALIVDSPARSYGRESNVLEERTFAPDRSSSSSSGSEHRSPLPITESNARNSQIPQRQLKRSNSTETNCSTSSTLTSGSQASAESLSRRVLKPQSVEAINRKNILASARCRSGRDLNGSPLIQRKFPEDECRATIVENGDDNYQRNRSKDATAKDVKIAYIEVTDSFGEAESFQKEANESKLPSKRSVVSSTVRSSKFETIEPSNDLKMWVRTEAKALARSAEERNNKIEKRSQEVDIVPANNPSKNSRSSSIEEQRLDSSSTNRNARNENSGSPEASKQRCGSNVLTSKNLLDDQNDLLTILTTKSRSRSAIHVDEGSFGRSKSQMSLEDILSAKTDPKLPRTPTNDSKIEKNTVLPAVQTRSLWESRDNRRVRRSSSTSNELWNEEKPLSKIPTLNRSRLNDSSGDETTEIERSKSLLSKIPTARSLARRSASVTDMKKALEKTEVNCTTGTQQTAHNRFPSLDSSVDENVSPVGTDVDTDRCCSEQFGSISSLASSTSLISQQELAQLVEEASLEEVRGAHDVIVVLLHKENHAGSVGITLAGGSDCEIKEITVHRVLAHSIADKDGRVQRGDRILSINGRSTQGLTHRESIAVLKQPRSEVVLVVSRARTEEGCKLRSRAASVETIIEGFETNDVTEDTAWGPPSVVAVYKDGAGLGFSLEGGRDSPLGNRPLLIKKIFTGGAAEKTGALKAGDQLLEVNGYDVTRMSRIEAWSLMKKLHDGEVNLLVRHPATKSS

Target ORF information:

RefSeq Version	XM_012281122.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pro-interleukin-16-like (LOC100882911), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281122.1

ORF Insert Sequence:

ATGGAGAACT ACAACGTTTA CGAGGACGAG GGACTAAGAA CGAATAAAGT ATTGGAGATA 
ATCGAAATAA GGAAGGCGAA GGCTTTCTCG CCGACGATCG CTAATTCTAA GGATGACAAG 
AGCAAAGGTA AGGGCGCCGA GAACGACCGA GTTAACGGCG ATAGCGAGAA CGTTCGAACG 
TATGAGAGAA TGAAACCGGA ATCGAGAAGC AGAACCATCT CGACGGAAGT CGAAGAAGAC 
TCGGAAGAGG AACACGAGAT GAGCACGAAA TTTAACGAGC CTATTGATTT TCAATGCGAG 
ACGACGGATC GTTCGGACGA TACGGTTTCG TCGTCGATCG ATGGACGATC GATCCAAGAT 
TCTTATCGTT CGTGCAACGA TATCGGTCCG CGTGAACCGA CCGTGAACGG AACAGGGAAC 
GAAAAGGGAA GCGTCGAAGA TGACGATACG AGCGAGGAAA GCGACGACGG CGATTACTAT 
TGGCAAAGTA ATTTGGCCAC CATAGGCGAG GAAGAGGAAA CGAATTCTCT CGAATACACG 
AACGCGAACA AAGAGTCGAG CGACAGCGAA AGTAACGCGA CGGAGAAATC GGAACGGCAG 
GAATCGCCGA GCAAGAAAGA CGCAAGTGAC ACGGTAAATG GTAGAATGGA CAACTGTGGA 
GGCGGTCAAC GCCTGCCCCC GGACGGGGAC GAGTTTCCAG CAGCCTATCA GGAGTTTGGC 
GGCAGTAACC AGCAGTACCG ATTCGTGACG GCGACGGCTA CTGCCCCGAG GACGTCGGTG 
ATGACGACGA ACGGCAGACT CGCCTGTAAC GAGGACGAGT CGTTCCTGGC TAACGAGAGT 
TTTAAACAGC GCGAGAACAA CGCGGTGAAC GCGACGAGCA CGATCCTGGT CGACGACAGA 
GTCGAAAACG TTTGTCAGGA GGTGGGCAAG ATCGGTTTAC CGAGCATCGA GATAAACGGC 
AAGACGAGTA TCGAGAACGT TTGCCAGGAC GCGAGCTCGT TGACACGAGA GTCTCGCGCG 
GACGACGCCA AACCGTTTGT CTCGGAAGAT TTCGTAAAAA AGACCGCGTC CATGACCAAC 
TATTCCAGCT ACGGAAACCA AAGCAACGGT AACGTGATCG TCTCGGAAAA GAAGATAGAA 
ATCACTGGAT ATTATCACAA AGACGTGCCA GTTATCGAAC AGTTTCGGGA CGAGAAGGAT 
GCTTTGGATA CGCCTCCGCC GCTTCCGCTC ACAGGTCCGC CTAAATTGGA GCCCGTCAGT 
CCATACGCGA GAAACGTCTC GGTTCTGGAA TCCTCGCCAA GGAAGTTCTC GTTGAACGGC 
GAGCTCTGGA GGAAGGACGA CAAGTCCGAG AAATCCGTCA GGGACAAGAT CGCGATGTTC 
TCGTCCCAGA GCAGCCTGGA TGGACCTCTG TTTCCGAACA CGATAACCGT CGCTACCGCG 
ACGAGTAACG CCAGGAAGCT GTCCAAGTAC AAATCCTCCG AGGACGTGTG CAACGACGAG 
AAGAGCTCTA GCCAGAAGGA GAGAGTCTCG TTTTTCGCGG ATCGGACGCA GAGCTCTTTC 
GATTTGACGG ACTCGGGCCG AAACGCGAAT CAGGAATCGG TGAAAAAGTA CAGACAAAGA 
TCGCCCAGTA TTTCTCAGAG TCCGTCGTTT GCTACGCCGA CTATTCACGC ATCGAAGACG 
TTTCCGATCG TTGCTCAGAA GCCGACCGCG TCGACGCCAA CGTTGATAAG CTCCTTCGAA 
ACGTCACCGA CATCCAAATC GCCGGTGAAG AGCTACTGCT CTTCCGCCGG CAGTCAAAAC 
TCTGCGATAA CCCCATTGTC GAAGCCCGTT ACGAGCACGG CGTTGTCTCG CGCTACCAGC 
TTCTCCGGAT CGAGCCCTTA CGGTCAAGAT CGAACCTTGG CGACGAACGA ATTGTCGAGC 
AGCTCTCAGA TCACCAGAAC GAATTCTTTG GCGTCGACGT TTAAGAAACC CAACGAGGAT 
ATCAGGAGAA ACAGCCTGAA TCAACTGATA GAACAGAGAA GGCGATCGAT ATCGAAATTG 
AGGGGACTGG TTATTCCGGA AAAAGAGAGC ATACCTATCG ATACGCCTAT CGTCGATTTG 
CCTGAAATTA AATCTCGAGA CTCCATACTA CTGCATCAGC TATCGAAGGC CAATATCCAA 
GACAAGTGGG GCTCCCAAAG TTCGTTGGCA AGCAACGCGA GCACGGCCAG TATGCCCGTG 
AAAACTACCT CCTTCAAGAT GCCGGCTCCT CAAATACACT CGAAATACTC TCCAGCTTTC 
AAAAGAAAGA GCTTGACAGT GTACGGTTCG TCCTCGACGA ATTGTTCGTT GTACGGTATC 
GCCGCGGTGA AAAGTAGTCC GATAGCGACG ACTACCGCGA CATCCACGCC GACGCTCTCC 
GAACCCCCAA AGAGTCTGGA GAGCATCTGC AGTCCGACGA GGAGCGATTA CAGTTTCGAG 
TTCGCGTCGA CGGCCAGTTC GCCCGAAGGT TCGCGCAACA GACCGCTCAG AACCGTTCAG 
AGAAAGACGC GAATGGATTA CGACGATTCC GACAACGATT CCGCCGTAAG CAGTTCGCAA 
AGCAGCATTT CGCGTGGCTT TAGCCCGCCT ATGTCCCCGG TACCTTCGGA ACGCTCCATC 
GTTTCCTCCA AACGATCCTA CGTCTCCACG GAGAACAACT ATCCGCGACG AGCTCTGATC 
GTCGACAGCC CAGCTAGAAG CTACGGCAGA GAATCCAACG TTCTCGAGGA GAGAACGTTC 
GCGCCTGACC GATCGTCGTC CAGTAGCAGC GGTTCCGAGC ACAGATCACC GTTGCCGATC 
ACCGAATCGA ACGCGAGAAA CTCTCAAATA CCTCAGAGAC AGTTAAAGCG ATCCAACAGT 
ACCGAGACCA ACTGCAGCAC GTCGTCGACT CTCACTTCCG GTTCTCAAGC GAGCGCGGAG 
TCTCTGTCGA GAAGAGTACT GAAGCCTCAG AGCGTGGAAG CCATCAATCG GAAGAACATT 
TTGGCGAGCG CCAGATGCCG AAGCGGCAGG GACCTGAACG GATCTCCGTT GATTCAACGA 
AAGTTCCCGG AGGACGAGTG TCGAGCGACC ATCGTCGAAA ACGGTGACGA TAACTACCAG 
AGGAATCGTT CGAAGGACGC GACCGCGAAA GACGTAAAGA TCGCGTACAT CGAGGTAACG 
GACTCGTTCG GGGAAGCCGA GTCTTTCCAG AAGGAGGCGA ACGAGTCGAA ATTGCCCTCG 
AAGAGAAGCG TCGTATCGTC CACCGTGCGG TCGTCCAAAT TCGAGACGAT AGAACCGTCC 
AACGACTTAA AAATGTGGGT TCGAACGGAA GCGAAAGCGT TGGCTAGATC GGCTGAAGAG 
AGGAACAACA AGATCGAGAA AAGATCTCAA GAGGTCGATA TCGTTCCTGC AAATAACCCT 
TCGAAGAACA GCAGATCGAG CAGCATCGAG GAACAACGGC TTGATTCATC GTCGACGAAC 
AGGAACGCGA GAAACGAAAA CTCCGGATCG CCGGAAGCTT CGAAACAACG ATGCGGTTCG 
AACGTATTAA CCTCGAAGAA TCTGTTGGAC GATCAGAACG ATCTTCTGAC GATTCTCACG 
ACCAAAAGTA GATCCAGATC GGCGATACAC GTGGACGAGG GAAGTTTCGG ACGATCGAAG 
AGCCAAATGA GTTTGGAGGA TATTCTGAGC GCGAAAACGG ATCCAAAATT ACCGCGCACA 
CCGACCAATG ATTCCAAGAT CGAGAAGAAC ACCGTACTTC CGGCTGTTCA GACCAGATCT 
CTGTGGGAGT CGAGGGACAA TCGACGCGTC AGGAGGAGCT CGTCGACCTC GAACGAACTA 
TGGAACGAGG AGAAGCCGCT TTCGAAGATA CCAACGTTGA ACAGGTCGAG ACTAAACGAT 
AGCAGCGGCG ACGAAACGAC GGAAATCGAA AGATCAAAGT CGTTGCTGTC GAAGATCCCA 
ACCGCGAGGA GCCTTGCTCG AAGAAGCGCC AGCGTCACGG ACATGAAGAA AGCCCTAGAG 
AAGACCGAGG TGAACTGTAC GACTGGTACG CAGCAGACTG CTCATAATCG ATTTCCGAGT 
CTGGACAGCA GCGTAGACGA GAACGTGTCA CCTGTTGGAA CGGACGTAGA CACGGATAGG 
TGCTGCAGCG AACAATTCGG TAGCATCAGT TCTTTGGCCA GTAGTACCAG CCTTATCTCG 
CAACAGGAAC TAGCTCAGTT AGTAGAGGAA GCGAGTCTGG AAGAGGTTCG AGGTGCTCAT 
GACGTGATCG TGGTATTACT GCACAAAGAG AATCATGCCG GTAGCGTTGG AATCACGTTG 
GCTGGCGGAA GCGATTGCGA GATCAAAGAA ATTACGGTTC ATCGAGTATT GGCGCATTCT 
ATAGCCGACA AGGACGGACG TGTGCAGAGG GGAGACAGAA TTTTAAGCAT AAACGGAAGA 
AGCACGCAAG GTCTCACGCA CAGGGAGAGC ATTGCGGTGC TTAAACAACC TAGGTCGGAA 
GTCGTCTTGG TAGTTTCTCG AGCGAGAACC GAGGAAGGTT GTAAATTGAG ATCAAGGGCA 
GCGAGCGTCG AAACTATTAT CGAAGGATTC GAGACGAACG ATGTCACGGA GGATACTGCG 
TGGGGACCGC CTTCGGTTGT AGCCGTGTAC AAAGATGGAG CTGGTTTGGG ATTCAGTCTG 
GAGGGAGGCA GAGACAGCCC TCTTGGAAAC AGACCGTTGT TGATCAAGAA AATATTTACC 
GGAGGTGCTG CCGAAAAAAC GGGTGCCTTA AAGGCAGGAG ATCAACTATT GGAAGTGAAC 
GGATACGATG TTACGCGTAT GTCGAGGATC GAAGCGTGGT CCCTTATGAA GAAACTACAC 
GACGGCGAAG TGAACCTCCT AGTCAGACAC CCTGCCACTA AATCCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

LOC100882911 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム