LOC100880906 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100880906
Entrez Gene ID	100880906
Full Name	116 kDa U5 small nuclear ribonucleoprotein component
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003704150.2	XP_003704198.1	116 kDa U5 small nuclear ribonucleoprotein component

Pathways from BioSystems
Gene Ontology

The following LOC100880906 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100880906 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe106520	XM_003704150.2 Latest version!	Megachile rotundata 116 kDa U5 small nuclear ribonucleoprotein component (LOC100880906), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100880906 ( XM_003704150.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003704198.1 Megachile rotundata 116 kDa U5 small nuclear ribonucleoprotein component (LOC100880906), mRNA.

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CloneID	OMe106520
Clone ID Related Accession (Same CDS sequence)	XM_003704150.2
Accession Version	XM_003704150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	116 kDa U5 small nuclear ribonucleoprotein component
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797193.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003704150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAG ATCTATATGA TGAATTTGGT AACTATATAG GCCCCGATTT AGCTTCTGAT 
AGTGAAGATG AAAACGAATA TGGAAATGCT GGCGATGACG CGGATGACAG AGAACGTTCA 
GATGAGGAGA TGGAAGAAGA TAAGGATGAA TCTAGAGAAC AACTGGAGCA AGGAAATTCT 
ATGGCAGTGG TACTTCACGA AGATAAAAGA TATTATCCCA GTGCTTTAGA AGTTTATGGA 
CCAGAGGTAG AAACTTTAGT ACAAGAAGAA GACGCGCAAC CATTAGATAA ACCATTGATT 
GCTCCAACCA GAAGACCAAA ATTTCAAATA AAACAGCAGC AACTTCCAGA AACAACATAC 
AGTATTGAAT TTTTAGCAGA TATGATGGAT GCACCTCATC TTATAAGGAA TGTTGTTTTA 
CTTGGTCACT TGCATCATGG TAAAACCACT TTGGTTGATT GTTTAGTGAG GCAAACACAT 
CCCTACTTAC ACAGTGTAAC AGATGAAAAG CCATTGAGAT ATACAGATAC TCTATTTACG 
GAGCAACAGC GAGGTGTATC GACAAAGGCC ACACCCGTGA CCCTACTACT TCAAGACGTG 
AAATCAAAAT CCTATCTCTT AAATATATTC GATACCCCGG GTCATGTGAA CTTTTCAGAC 
GAAGCTACAG CAGCTATACG TTTATCCGAC GGAGCGGTAC TAATTGTCGA TGCTGCTGAA 
GGTGTCATGT TAAACACGGA ACGTCTGCTA AAACACGCGC TCCAAGAGAA ACTAGCGTTA 
ACGGTGTGCA TAAACAAGAT AGATCGTCTC ATTCTAGAAC TGAAGTTGCC TCCGCTCGAT 
GCATATTACA AACTGAGACA TATCATCGAA GAGATCAATG GACTTATAGC ACTATACTCG 
GACTCAGAGA ATCCGTCTTT TGTCTCACCA GCGATCGGAA ACGTATGTTT CGCGAGTTCC 
GAATACAATG TCTGTTTTAC CCTCAAATCT TTCGCGGCAC TTTACGCCAA GACACATCTC 
ACTCTTAACG CCAACGAGTT CGCGAAAAGA CTATGGGGCG ACATATATTT TAACTCGAAA 
ACACGAAAAT TTACGAAAAA ACCACCGCAT AATACTGCTC AGCGCAGTTT CATCGAGTTC 
ATTCTCGAAC CCTTGTACAA AATATTCGCA CAAGTGGTCG GTGATGTCGA CACGACGTTA 
CCCGATGTGT TGGATGAACT GGGCATAAGA TTAACATCCG AGGAAATGAA AATGAACATA 
AGACCTCTGT TGAGGCTGGT GTGTACACGT TTCTTGGGCG ATATGTGTGG ATTAGTAGAC 
ATGTGTGTAG CTCATGTTCC CAGTCCTCAG TCGCATGCAC CGGTCAAAGT TCAACACGTA 
TACACGGGAC CAATGGATTC GCCTTTAGCG CAGGATATGA TTAATTGCGA TCCTGATGGA 
AGATTGATGA TACACAGTAC AAAAATGTAT CCCACGGAGG ACTGCACGCT GTTCGTAGTT 
CTAGGAAGAG TCATGTCCGG AACTCTCGAG GCAGGACAAC GTGTCCGTGT GTTGGGTGAA 
GCGTACTCGC GTACGGACGA AGAGGATTCT CGCGTTCTTA CAGTAGGAAG GCTATGGATC 
AGTGAGGCGC GCTATTCGAT CGAATTGAAC AGAGTACCTG CGGGCAATTG GGTACTTATC 
GAGGGCATCG ATAGACCTAT CGTGAAAACC AGCACGATTA CCGATCTTAA AAATTCGGAC 
GATCTACACA TTTTCCGACC ATTGAAATTT AACACACAGA GCGTAATCAA AATAGCGGTC 
GAACCTGTTA ATCCATCAGA GTTGCCAAAA ATGTTAGATG GTCTGAGAAA GGTGAACAAA 
AGTTATCCTT TGTTGGGCAC CAGGGTCGAA GAGAGTGGAG AACACGTGGT CTTAGGAACG 
GGTGAATTGT ACTTGGACTG TGCGATGCAC GATTTACGTC GCATGTACTC CGAGATCGAT 
ATAAAAGTGG CAGATCCTGT GGTGGCTTTC GCAGAAACAG TTGTAGAGAC AAGTTCCCTT 
AAATGTTTCG CCGAAACGCC AAATAAACGA AACAAGTTAA CCATGATTGC CGAGCCTCTC 
GAAAGAGGTC TTGCCGAAGA TATAGAGGCA GAACATGTTC GAATTACGTG GAATAAGAAG 
CGGTTAGGAG AGTTCTTCCA AACAAAGTAC GACTGGGATT TGCTGGCCGC TCGAAGTATA 
TGGGCGTTTG GACCAGATGC CACTGGACCC AACATTCTGG TCGACGACAC GTTGCCATCG 
GAAGTGGACA AGACATTACT GAACAGTGCT CGTGACGCCA TCATTCAAGG ATTTCAGTGG 
GGTACACGAG AGGGTCCACT TTGCGAGGAA CCGATTAGAA ACGTGAAATT CAAAATCCTG 
GATGCCGTGA TTGCTCAGGA ACCACTTCAT CGAGGAGGTG GTCAAATAAT TCCAACTGCC 
AGGAGGGTAG CGTACTCCGC TTTCCTCATG GCAACACCCA GATTGATGGA ACCTTATTTA 
TTCGTCGAAG TCCAAGCCCC CGCCGATTGC GTGTCGGCCG TTTATACGGT ACTGGCTAAA 
AGAAGAGGTC ACGTGACCCA AGACGCTCCC GTTCCCGGAA GTCCCTTGTA CACCATCAAG 
GCTTTCATAC CGGCGATCGA TAGCTTCGGC TTTGAGACCG ACTTACGGAC GCACACGCAA 
GGCCAGGCGT TCTGTCTGTC CGTGTTTCAC CATTGGCAGA TTGTACCTGG CGATCCTTTG 
GATAAAAGCA TAACTATACG ACCACTCGAG CCGCAGCCAG CCACTCATTT AGCTAGGGAA 
TTTATGCTGA AAACACGAAG ACGGAAGGGC CTGTCCGAGG ATGTGTCTAT CAATAAATTC 
TTCGACGATC CTATGTTATT GGAATTAGCT AGACAAGATG TATTACTCAA TTATCCACTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003704198.1
CDS	254..3196
Translation	MDADLYDEFGNYIGPDLASDSEDENEYGNAGDDADDRERSDEEMEEDKDESREQLEQGNSMAVVLHEDKRYYPSALEVYGPEVETLVQEEDAQPLDKPLIAPTRRPKFQIKQQQLPETTYSIEFLADMMDAPHLIRNVVLLGHLHHGKTTLVDCLVRQTHPYLHSVTDEKPLRYTDTLFTEQQRGVSTKATPVTLLLQDVKSKSYLLNIFDTPGHVNFSDEATAAIRLSDGAVLIVDAAEGVMLNTERLLKHALQEKLALTVCINKIDRLILELKLPPLDAYYKLRHIIEEINGLIALYSDSENPSFVSPAIGNVCFASSEYNVCFTLKSFAALYAKTHLTLNANEFAKRLWGDIYFNSKTRKFTKKPPHNTAQRSFIEFILEPLYKIFAQVVGDVDTTLPDVLDELGIRLTSEEMKMNIRPLLRLVCTRFLGDMCGLVDMCVAHVPSPQSHAPVKVQHVYTGPMDSPLAQDMINCDPDGRLMIHSTKMYPTEDCTLFVVLGRVMSGTLEAGQRVRVLGEAYSRTDEEDSRVLTVGRLWISEARYSIELNRVPAGNWVLIEGIDRPIVKTSTITDLKNSDDLHIFRPLKFNTQSVIKIAVEPVNPSELPKMLDGLRKVNKSYPLLGTRVEESGEHVVLGTGELYLDCAMHDLRRMYSEIDIKVADPVVAFAETVVETSSLKCFAETPNKRNKLTMIAEPLERGLAEDIEAEHVRITWNKKRLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKTLLNSARDAIIQGFQWGTREGPLCEEPIRNVKFKILDAVIAQEPLHRGGGQIIPTARRVAYSAFLMATPRLMEPYLFVEVQAPADCVSAVYTVLAKRRGHVTQDAPVPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFCLSVFHHWQIVPGDPLDKSITIRPLEPQPATHLAREFMLKTRRRKGLSEDVSINKFFDDPMLLELARQDVLLNYPL

Target ORF information:

RefSeq Version	XM_003704150.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata 116 kDa U5 small nuclear ribonucleoprotein component (LOC100880906), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003704150.2

ORF Insert Sequence:

ATGGATGCAG ATCTATATGA TGAATTTGGT AACTATATAG GCCCCGATTT AGCTTCTGAT 
AGTGAAGATG AAAACGAATA TGGAAATGCT GGCGATGACG CGGATGACAG AGAACGTTCA 
GATGAGGAGA TGGAAGAAGA TAAGGATGAA TCTAGAGAAC AACTGGAGCA AGGAAATTCT 
ATGGCAGTGG TACTTCACGA AGATAAAAGA TATTATCCCA GTGCTTTAGA AGTTTATGGA 
CCAGAGGTAG AAACTTTAGT ACAAGAAGAA GACGCGCAAC CATTAGATAA ACCATTGATT 
GCTCCAACCA GAAGACCAAA ATTTCAAATA AAACAGCAGC AACTTCCAGA AACAACATAC 
AGTATTGAAT TTTTAGCAGA TATGATGGAT GCACCTCATC TTATAAGGAA TGTTGTTTTA 
CTTGGTCACT TGCATCATGG TAAAACCACT TTGGTTGATT GTTTAGTGAG GCAAACACAT 
CCCTACTTAC ACAGTGTAAC AGATGAAAAG CCATTGAGAT ATACAGATAC TCTATTTACG 
GAGCAACAGC GAGGTGTATC GACAAAGGCC ACACCCGTGA CCCTACTACT TCAAGACGTG 
AAATCAAAAT CCTATCTCTT AAATATATTC GATACCCCGG GTCATGTGAA CTTTTCAGAC 
GAAGCTACAG CAGCTATACG TTTATCCGAC GGAGCGGTAC TAATTGTCGA TGCTGCTGAA 
GGTGTCATGT TAAACACGGA ACGTCTGCTA AAACACGCGC TCCAAGAGAA ACTAGCGTTA 
ACGGTGTGCA TAAACAAGAT AGATCGTCTC ATTCTAGAAC TGAAGTTGCC TCCGCTCGAT 
GCATATTACA AACTGAGACA TATCATCGAA GAGATCAATG GACTTATAGC ACTATACTCG 
GACTCAGAGA ATCCGTCTTT TGTCTCACCA GCGATCGGAA ACGTATGTTT CGCGAGTTCC 
GAATACAATG TCTGTTTTAC CCTCAAATCT TTCGCGGCAC TTTACGCCAA GACACATCTC 
ACTCTTAACG CCAACGAGTT CGCGAAAAGA CTATGGGGCG ACATATATTT TAACTCGAAA 
ACACGAAAAT TTACGAAAAA ACCACCGCAT AATACTGCTC AGCGCAGTTT CATCGAGTTC 
ATTCTCGAAC CCTTGTACAA AATATTCGCA CAAGTGGTCG GTGATGTCGA CACGACGTTA 
CCCGATGTGT TGGATGAACT GGGCATAAGA TTAACATCCG AGGAAATGAA AATGAACATA 
AGACCTCTGT TGAGGCTGGT GTGTACACGT TTCTTGGGCG ATATGTGTGG ATTAGTAGAC 
ATGTGTGTAG CTCATGTTCC CAGTCCTCAG TCGCATGCAC CGGTCAAAGT TCAACACGTA 
TACACGGGAC CAATGGATTC GCCTTTAGCG CAGGATATGA TTAATTGCGA TCCTGATGGA 
AGATTGATGA TACACAGTAC AAAAATGTAT CCCACGGAGG ACTGCACGCT GTTCGTAGTT 
CTAGGAAGAG TCATGTCCGG AACTCTCGAG GCAGGACAAC GTGTCCGTGT GTTGGGTGAA 
GCGTACTCGC GTACGGACGA AGAGGATTCT CGCGTTCTTA CAGTAGGAAG GCTATGGATC 
AGTGAGGCGC GCTATTCGAT CGAATTGAAC AGAGTACCTG CGGGCAATTG GGTACTTATC 
GAGGGCATCG ATAGACCTAT CGTGAAAACC AGCACGATTA CCGATCTTAA AAATTCGGAC 
GATCTACACA TTTTCCGACC ATTGAAATTT AACACACAGA GCGTAATCAA AATAGCGGTC 
GAACCTGTTA ATCCATCAGA GTTGCCAAAA ATGTTAGATG GTCTGAGAAA GGTGAACAAA 
AGTTATCCTT TGTTGGGCAC CAGGGTCGAA GAGAGTGGAG AACACGTGGT CTTAGGAACG 
GGTGAATTGT ACTTGGACTG TGCGATGCAC GATTTACGTC GCATGTACTC CGAGATCGAT 
ATAAAAGTGG CAGATCCTGT GGTGGCTTTC GCAGAAACAG TTGTAGAGAC AAGTTCCCTT 
AAATGTTTCG CCGAAACGCC AAATAAACGA AACAAGTTAA CCATGATTGC CGAGCCTCTC 
GAAAGAGGTC TTGCCGAAGA TATAGAGGCA GAACATGTTC GAATTACGTG GAATAAGAAG 
CGGTTAGGAG AGTTCTTCCA AACAAAGTAC GACTGGGATT TGCTGGCCGC TCGAAGTATA 
TGGGCGTTTG GACCAGATGC CACTGGACCC AACATTCTGG TCGACGACAC GTTGCCATCG 
GAAGTGGACA AGACATTACT GAACAGTGCT CGTGACGCCA TCATTCAAGG ATTTCAGTGG 
GGTACACGAG AGGGTCCACT TTGCGAGGAA CCGATTAGAA ACGTGAAATT CAAAATCCTG 
GATGCCGTGA TTGCTCAGGA ACCACTTCAT CGAGGAGGTG GTCAAATAAT TCCAACTGCC 
AGGAGGGTAG CGTACTCCGC TTTCCTCATG GCAACACCCA GATTGATGGA ACCTTATTTA 
TTCGTCGAAG TCCAAGCCCC CGCCGATTGC GTGTCGGCCG TTTATACGGT ACTGGCTAAA 
AGAAGAGGTC ACGTGACCCA AGACGCTCCC GTTCCCGGAA GTCCCTTGTA CACCATCAAG 
GCTTTCATAC CGGCGATCGA TAGCTTCGGC TTTGAGACCG ACTTACGGAC GCACACGCAA 
GGCCAGGCGT TCTGTCTGTC CGTGTTTCAC CATTGGCAGA TTGTACCTGG CGATCCTTTG 
GATAAAAGCA TAACTATACG ACCACTCGAG CCGCAGCCAG CCACTCATTT AGCTAGGGAA 
TTTATGCTGA AAACACGAAG ACGGAAGGGC CTGTCCGAGG ATGTGTCTAT CAATAAATTC 
TTCGACGATC CTATGTTATT GGAATTAGCT AGACAAGATG TATTACTCAA TTATCCACTG 
TAA