LOC100875284 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100875284
Entrez Gene ID	100875284
Full Name	interferon-inducible double-stranded RNA-dependent protein kinase activator A-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012296459.1	XP_012151849.1	interferon-inducible double-stranded RNA-dependent protein kinase activator A-like isoform X1
XM_003707994.2	XP_003708042.2	interferon-inducible double-stranded RNA-dependent protein kinase activator A-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100875284 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100875284 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe114420	XM_012296459.1 Latest version!	Megachile rotundata interferon-inducible double-stranded RNA-dependent protein kinase activator A-like (LOC100875284), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OMe114421	XM_003707994.2 Latest version!	Megachile rotundata interferon-inducible double-stranded RNA-dependent protein kinase activator A-like (LOC100875284), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100875284 ( XM_012296459.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151849.1 Megachile rotundata interferon-inducible double-stranded RNA-dependent protein kinase activator A-like (LOC100875284), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe114420
Clone ID Related Accession (Same CDS sequence)	XM_012296459.1
Accession Version	XM_012296459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	interferon-inducible double-stranded RNA-dependent protein kinase activator A-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797732.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAA CACCTGTGTC TATTTTACAA GAAATGATGG TGAAAAAACA CACAGTACCT 
AATTATGAAC TTATACATGA TGGTGGAGGT ACTCACATGA ATACTTTTAC GTACAAAGTA 
ATTTGTGATG GCCTTACTGC AACTGGCACA GGACGTTCTA AAAAAGATGC TAAACACGAA 
GCAGCAAAAG CAATGCTAGA AACTATTGCA GCACATAGAG GTTATCCACA GTTACCAGCA 
TCTCCAGCGC AATCACCTGT TAGAACACCT TTGCCTCCAC AAGTTCCTGA AGTACCAAGA 
ATTCCACCAA CTGAACCATT TGTTAATGCT ATTGGTGCTT TACAAGATTT GTGTGCAGAA 
AATAATTTGC AAGAACCTTT ATATGTACCA ATTAGCGATG TTGGACCCCC ACATGCAAAG 
ATTTTTACTA TTCAATGTGA AGTATCAACA TTTAAAGAAA TTGGAGTTGC TAAGACAAAA 
AAGCAAGCGA AACAGGAAGC TGCTAAAAAA ATGTTGGACA AAATAAGTGA TTTAGTGGCA 
AATCAAGATG AAAATTTGTC TGAAGAAAAT GATGATACTA CTAAGAAGGA AAATATTGCT 
AATGAACTTG CAAAAGCTCG TTATCCCTTA TTATCAAGGT TACCAACAAA AAAAGTAAAT 
TTAGGTGTTA AGTTATCGGA GTATCATATC AAACTAAAAA ATAGTTTCCC TAGTGATATT 
TATACTGACT TAATTGAGAA ATTAGAATCC TTTATTCCTA AAGATAAAAG TAATGTTTCT 
GAGGAATATG TAGAAACATT ATTAAGCGAA TTCTCAGAAT TCTTATCAAC TTTAGGTCTA 
AATATTTCAA ATGTGACTTT AGAGTCTAAC AAAGCAGATA ATCACGTTGT ACTTATGAAA 
ATAAATACTT TTCCTGAAAT TACCCAAATT TCATGTGCAG ATACAGACAC ACAAGCTAGG 
TTTTTTGCAA TAATAAACCT AATTAATACC ATGTTATTTT TGTTGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151849.1
CDS	158..1168
Translation	MNKTPVSILQEMMVKKHTVPNYELIHDGGGTHMNTFTYKVICDGLTATGTGRSKKDAKHEAAKAMLETIAAHRGYPQLPASPAQSPVRTPLPPQVPEVPRIPPTEPFVNAIGALQDLCAENNLQEPLYVPISDVGPPHAKIFTIQCEVSTFKEIGVAKTKKQAKQEAAKKMLDKISDLVANQDENLSEENDDTTKKENIANELAKARYPLLSRLPTKKVNLGVKLSEYHIKLKNSFPSDIYTDLIEKLESFIPKDKSNVSEEYVETLLSEFSEFLSTLGLNISNVTLESNKADNHVVLMKINTFPEITQISCADTDTQARFFAIINLINTMLFLLK

Target ORF information:

RefSeq Version	XM_012296459.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata interferon-inducible double-stranded RNA-dependent protein kinase activator A-like (LOC100875284), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296459.1

ORF Insert Sequence:

ATGAATAAAA CACCTGTGTC TATTTTACAA GAAATGATGG TGAAAAAACA CACAGTACCT 
AATTATGAAC TTATACATGA TGGTGGAGGT ACTCACATGA ATACTTTTAC GTACAAAGTA 
ATTTGTGATG GCCTTACTGC AACTGGCACA GGACGTTCTA AAAAAGATGC TAAACACGAA 
GCAGCAAAAG CAATGCTAGA AACTATTGCA GCACATAGAG GTTATCCACA GTTACCAGCA 
TCTCCAGCGC AATCACCTGT TAGAACACCT TTGCCTCCAC AAGTTCCTGA AGTACCAAGA 
ATTCCACCAA CTGAACCATT TGTTAATGCT ATTGGTGCTT TACAAGATTT GTGTGCAGAA 
AATAATTTGC AAGAACCTTT ATATGTACCA ATTAGCGATG TTGGACCCCC ACATGCAAAG 
ATTTTTACTA TTCAATGTGA AGTATCAACA TTTAAAGAAA TTGGAGTTGC TAAGACAAAA 
AAGCAAGCGA AACAGGAAGC TGCTAAAAAA ATGTTGGACA AAATAAGTGA TTTAGTGGCA 
AATCAAGATG AAAATTTGTC TGAAGAAAAT GATGATACTA CTAAGAAGGA AAATATTGCT 
AATGAACTTG CAAAAGCTCG TTATCCCTTA TTATCAAGGT TACCAACAAA AAAAGTAAAT 
TTAGGTGTTA AGTTATCGGA GTATCATATC AAACTAAAAA ATAGTTTCCC TAGTGATATT 
TATACTGACT TAATTGAGAA ATTAGAATCC TTTATTCCTA AAGATAAAAG TAATGTTTCT 
GAGGAATATG TAGAAACATT ATTAAGCGAA TTCTCAGAAT TCTTATCAAC TTTAGGTCTA 
AATATTTCAA ATGTGACTTT AGAGTCTAAC AAAGCAGATA ATCACGTTGT ACTTATGAAA 
ATAAATACTT TTCCTGAAAT TACCCAAATT TCATGTGCAG ATACAGACAC ACAAGCTAGG 
TTTTTTGCAA TAATAAACCT AATTAATACC ATGTTATTTT TGTTGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100875284 ( XM_003707994.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003708042.2 Megachile rotundata interferon-inducible double-stranded RNA-dependent protein kinase activator A-like (LOC100875284), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe114421
Clone ID Related Accession (Same CDS sequence)	XM_003707994.2
Accession Version	XM_003707994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	interferon-inducible double-stranded RNA-dependent protein kinase activator A-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797732.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003707994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTTAG AAATGATGGT GAAAAAACAC ACAGTACCTA ATTATGAACT TATACATGAT 
GGTGGAGGTA CTCACATGAA TACTTTTACG TACAAAGTAA TTTGTGATGG CCTTACTGCA 
ACTGGCACAG GACGTTCTAA AAAAGATGCT AAACACGAAG CAGCAAAAGC AATGCTAGAA 
ACTATTGCAG CACATAGAGG TTATCCACAG TTACCAGCAT CTCCAGCGCA ATCACCTGTT 
AGAACACCTT TGCCTCCACA AGTTCCTGAA GTACCAAGAA TTCCACCAAC TGAACCATTT 
GTTAATGCTA TTGGTGCTTT ACAAGATTTG TGTGCAGAAA ATAATTTGCA AGAACCTTTA 
TATGTACCAA TTAGCGATGT TGGACCCCCA CATGCAAAGA TTTTTACTAT TCAATGTGAA 
GTATCAACAT TTAAAGAAAT TGGAGTTGCT AAGACAAAAA AGCAAGCGAA ACAGGAAGCT 
GCTAAAAAAA TGTTGGACAA AATAAGTGAT TTAGTGGCAA ATCAAGATGA AAATTTGTCT 
GAAGAAAATG ATGATACTAC TAAGAAGGAA AATATTGCTA ATGAACTTGC AAAAGCTCGT 
TATCCCTTAT TATCAAGGTT ACCAACAAAA AAAGTAAATT TAGGTGTTAA GTTATCGGAG 
TATCATATCA AACTAAAAAA TAGTTTCCCT AGTGATATTT ATACTGACTT AATTGAGAAA 
TTAGAATCCT TTATTCCTAA AGATAAAAGT AATGTTTCTG AGGAATATGT AGAAACATTA 
TTAAGCGAAT TCTCAGAATT CTTATCAACT TTAGGTCTAA ATATTTCAAA TGTGACTTTA 
GAGTCTAACA AAGCAGATAA TCACGTTGTA CTTATGAAAA TAAATACTTT TCCTGAAATT 
ACCCAAATTT CATGTGCAGA TACAGACACA CAAGCTAGGT TTTTTGCAAT AATAAACCTA 
ATTAATACCA TGTTATTTTT GTTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003708042.2
CDS	33..1022
Translation	MYLEMMVKKHTVPNYELIHDGGGTHMNTFTYKVICDGLTATGTGRSKKDAKHEAAKAMLETIAAHRGYPQLPASPAQSPVRTPLPPQVPEVPRIPPTEPFVNAIGALQDLCAENNLQEPLYVPISDVGPPHAKIFTIQCEVSTFKEIGVAKTKKQAKQEAAKKMLDKISDLVANQDENLSEENDDTTKKENIANELAKARYPLLSRLPTKKVNLGVKLSEYHIKLKNSFPSDIYTDLIEKLESFIPKDKSNVSEEYVETLLSEFSEFLSTLGLNISNVTLESNKADNHVVLMKINTFPEITQISCADTDTQARFFAIINLINTMLFLLK

Target ORF information:

RefSeq Version	XM_003707994.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata interferon-inducible double-stranded RNA-dependent protein kinase activator A-like (LOC100875284), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003707994.2

ORF Insert Sequence:

ATGTATTTAG AAATGATGGT GAAAAAACAC ACAGTACCTA ATTATGAACT TATACATGAT 
GGTGGAGGTA CTCACATGAA TACTTTTACG TACAAAGTAA TTTGTGATGG CCTTACTGCA 
ACTGGCACAG GACGTTCTAA AAAAGATGCT AAACACGAAG CAGCAAAAGC AATGCTAGAA 
ACTATTGCAG CACATAGAGG TTATCCACAG TTACCAGCAT CTCCAGCGCA ATCACCTGTT 
AGAACACCTT TGCCTCCACA AGTTCCTGAA GTACCAAGAA TTCCACCAAC TGAACCATTT 
GTTAATGCTA TTGGTGCTTT ACAAGATTTG TGTGCAGAAA ATAATTTGCA AGAACCTTTA 
TATGTACCAA TTAGCGATGT TGGACCCCCA CATGCAAAGA TTTTTACTAT TCAATGTGAA 
GTATCAACAT TTAAAGAAAT TGGAGTTGCT AAGACAAAAA AGCAAGCGAA ACAGGAAGCT 
GCTAAAAAAA TGTTGGACAA AATAAGTGAT TTAGTGGCAA ATCAAGATGA AAATTTGTCT 
GAAGAAAATG ATGATACTAC TAAGAAGGAA AATATTGCTA ATGAACTTGC AAAAGCTCGT 
TATCCCTTAT TATCAAGGTT ACCAACAAAA AAAGTAAATT TAGGTGTTAA GTTATCGGAG 
TATCATATCA AACTAAAAAA TAGTTTCCCT AGTGATATTT ATACTGACTT AATTGAGAAA 
TTAGAATCCT TTATTCCTAA AGATAAAAGT AATGTTTCTG AGGAATATGT AGAAACATTA 
TTAAGCGAAT TCTCAGAATT CTTATCAACT TTAGGTCTAA ATATTTCAAA TGTGACTTTA 
GAGTCTAACA AAGCAGATAA TCACGTTGTA CTTATGAAAA TAAATACTTT TCCTGAAATT 
ACCCAAATTT CATGTGCAGA TACAGACACA CAAGCTAGGT TTTTTGCAAT AATAAACCTA 
ATTAATACCA TGTTATTTTT GTTGAAGTAG