ORF » Species Summary » Medicago truncatula » LOC25500823 cDNA ORF clone

LOC25500823 cDNA ORF clone, Medicago truncatula(barrel medic)

The following LOC25500823 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25500823 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe164711 XM_013589317.2
Latest version!		 Medicago truncatula 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial (LOC25500823), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
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OMe164711 XM_013589317.1 Medicago truncatula 3-methyl-2-oxobutanoate hydroxymethyltransferase partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC25500823 ( XM_013589317.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013444771.1 Medicago truncatula 3-methyl-2-oxobutanoate hydroxymethyltransferase partial mRNA.
    Price & Availability↑
    CloneID OMe164711
    Clone ID Related Accession (Same CDS sequence) XM_013589317.1 , XM_013589317.2
    Accession Version XM_013589317.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-08-24
    Organism Medicago truncatula(barrel medic)
    Product 3-methyl-2-oxobutanoate hydroxymethyltransferase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016414). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAATTC TAAGATCAAT GCTGAAAGCA ACAAAAACCC TTCATCGTTC ATCATCTCTT 
    CTCCGTAACA TGAGCAATCT CCCAGAAAAC ACAGTATACA CAGGTCCAAC AACACAAAAC 
    AAAAGAGTAA CACTTTCACA ACTACAACAA AAACATAAAA ACTCACAACC AATCACAATG 
    GTCACTGCTT ATGATTACCC TTCTGCTGTT CACCTCGATA TGGCCGCCAT TGATATCTGT 
    CTTGTTGGTG ATTCTGCTTC CATGGTTGTT CATGGTCATG ATACTACTTT GCCTATTACT 
    TTAGATGAAA TGCTTGTTCA TTGTCGTGCA GTTGCTCGTG GTGCTAAAAC TCCTCTTCTT 
    GTTGGTGATT TGCCTTTTGG AACCTATGAA TGTAGTTCTA ATCAGGCAGT GGACACGGCA 
    GTTCGAATTT TGAAAGAAGG ACAAATGGAT GCCATAAAGT TGGAAGGAGG TTCACCTTCA 
    AGAATTGTTG CAGCAAAAGC TATTGTTGAA GCTGGAATAG CTGTGATTGG GCATGTTGGT 
    CTTACTCCAC AAGCCATTAG TGTTTTAGGA GGATTTAGGC CTCAGGGTAG GAATGTTGCA 
    AGTGCTGTCA AGGTTGTCGA GACAGCATTG GCTTTGCAAG AAGCAGGGTG TTTTGCTGTT 
    GTTCTTGAAT GTGTGCCTGC ACCGGTGACT GCAGCAGCAA CAGCGGCGCT TCAAATTCCT 
    ACAATTGGAA TTGGGGCTGG TCCCTATTGC AGTGGACAGG TGCTTGTTTA CCATGATCTT 
    CTTGGTATGT TACAACACCC TCACCATGCA AAGGTGACTC CAAAATTTTG TAAACAGTAC 
    GCTCGTGTTG GAGACGTCAT CAATAAAGCC TTACTGGAAT ATAAGGAAGA TGTGATGAAT 
    GGTTCATTTC CTGATGCTCA ACATAGTCCA TACAAAATCA GTGAAACTGA TGCTAATGGT 
    TTCTTAAATG AGCTGCAAAA GTTAGGTTTT GACAAGGCTG CATCTGCGGC ATCTGAAGCA 
    GTTCAGAAGA TGGTTTCAAA ATCAACAAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013444771.1
    CDS1..1053
    Translation

    Target ORF information:

    RefSeq Version XM_013589317.1
    Organism Medicago truncatula(barrel medic)
    Definition Medicago truncatula 3-methyl-2-oxobutanoate hydroxymethyltransferase partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_013589317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAATTC TAAGATCAAT GCTGAAAGCA ACAAAAACCC TTCATCGTTC ATCATCTCTT 
    CTCCGTAACA TGAGCAATCT CCCAGAAAAC ACAGTATACA CAGGTCCAAC AACACAAAAC 
    AAAAGAGTAA CACTTTCACA ACTACAACAA AAACATAAAA ACTCACAACC AATCACAATG 
    GTCACTGCTT ATGATTACCC TTCTGCTGTT CACCTCGATA TGGCCGCCAT TGATATCTGT 
    CTTGTTGGTG ATTCTGCTTC CATGGTTGTT CATGGTCATG ATACTACTTT GCCTATTACT 
    TTAGATGAAA TGCTTGTTCA TTGTCGTGCA GTTGCTCGTG GTGCTAAAAC TCCTCTTCTT 
    GTTGGTGATT TGCCTTTTGG AACCTATGAA TGTAGTTCTA ATCAGGCAGT GGACACGGCA 
    GTTCGAATTT TGAAAGAAGG ACAAATGGAT GCCATAAAGT TGGAAGGAGG TTCACCTTCA 
    AGAATTGTTG CAGCAAAAGC TATTGTTGAA GCTGGAATAG CTGTGATTGG GCATGTTGGT 
    CTTACTCCAC AAGCCATTAG TGTTTTAGGA GGATTTAGGC CTCAGGGTAG GAATGTTGCA 
    AGTGCTGTCA AGGTTGTCGA GACAGCATTG GCTTTGCAAG AAGCAGGGTG TTTTGCTGTT 
    GTTCTTGAAT GTGTGCCTGC ACCGGTGACT GCAGCAGCAA CAGCGGCGCT TCAAATTCCT 
    ACAATTGGAA TTGGGGCTGG TCCCTATTGC AGTGGACAGG TGCTTGTTTA CCATGATCTT 
    CTTGGTATGT TACAACACCC TCACCATGCA AAGGTGACTC CAAAATTTTG TAAACAGTAC 
    GCTCGTGTTG GAGACGTCAT CAATAAAGCC TTACTGGAAT ATAAGGAAGA TGTGATGAAT 
    GGTTCATTTC CTGATGCTCA ACATAGTCCA TACAAAATCA GTGAAACTGA TGCTAATGGT 
    TTCTTAAATG AGCTGCAAAA GTTAGGTTTT GACAAGGCTG CATCTGCGGC ATCTGAAGCA 
    GTTCAGAAGA TGGTTTCAAA ATCAACAAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC25500823 ( XM_013589317.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013444771.1 Medicago truncatula 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial (LOC25500823), mRNA.
    Price & Availability↑
    CloneID OMe164711
    Clone ID Related Accession (Same CDS sequence) XM_013589317.1 , XM_013589317.2
    Accession Version XM_013589317.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-22
    Organism Medicago truncatula(barrel medic)
    Product 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016414.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013589317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAATTC TAAGATCAAT GCTGAAAGCA ACAAAAACCC TTCATCGTTC ATCATCTCTT 
    CTCCGTAACA TGAGCAATCT CCCAGAAAAC ACAGTATACA CAGGTCCAAC AACACAAAAC 
    AAAAGAGTAA CACTTTCACA ACTACAACAA AAACATAAAA ACTCACAACC AATCACAATG 
    GTCACTGCTT ATGATTACCC TTCTGCTGTT CACCTCGATA TGGCCGCCAT TGATATCTGT 
    CTTGTTGGTG ATTCTGCTTC CATGGTTGTT CATGGTCATG ATACTACTTT GCCTATTACT 
    TTAGATGAAA TGCTTGTTCA TTGTCGTGCA GTTGCTCGTG GTGCTAAAAC TCCTCTTCTT 
    GTTGGTGATT TGCCTTTTGG AACCTATGAA TGTAGTTCTA ATCAGGCAGT GGACACGGCA 
    GTTCGAATTT TGAAAGAAGG ACAAATGGAT GCCATAAAGT TGGAAGGAGG TTCACCTTCA 
    AGAATTGTTG CAGCAAAAGC TATTGTTGAA GCTGGAATAG CTGTGATTGG GCATGTTGGT 
    CTTACTCCAC AAGCCATTAG TGTTTTAGGA GGATTTAGGC CTCAGGGTAG GAATGTTGCA 
    AGTGCTGTCA AGGTTGTCGA GACAGCATTG GCTTTGCAAG AAGCAGGGTG TTTTGCTGTT 
    GTTCTTGAAT GTGTGCCTGC ACCGGTGACT GCAGCAGCAA CAGCGGCGCT TCAAATTCCT 
    ACAATTGGAA TTGGGGCTGG TCCCTATTGC AGTGGACAGG TGCTTGTTTA CCATGATCTT 
    CTTGGTATGT TACAACACCC TCACCATGCA AAGGTGACTC CAAAATTTTG TAAACAGTAC 
    GCTCGTGTTG GAGACGTCAT CAATAAAGCC TTACTGGAAT ATAAGGAAGA TGTGATGAAT 
    GGTTCATTTC CTGATGCTCA ACATAGTCCA TACAAAATCA GTGAAACTGA TGCTAATGGT 
    TTCTTAAATG AGCTGCAAAA GTTAGGTTTT GACAAGGCTG CATCTGCGGC ATCTGAAGCA 
    GTTCAGAAGA TGGTTTCAAA ATCAACAAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013444771.1
    CDS843..1895
    Translation

    Target ORF information:

    RefSeq Version XM_013589317.2
    Organism Medicago truncatula(barrel medic)
    Definition Medicago truncatula 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial (LOC25500823), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_013589317.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAATTC TAAGATCAAT GCTGAAAGCA ACAAAAACCC TTCATCGTTC ATCATCTCTT 
    CTCCGTAACA TGAGCAATCT CCCAGAAAAC ACAGTATACA CAGGTCCAAC AACACAAAAC 
    AAAAGAGTAA CACTTTCACA ACTACAACAA AAACATAAAA ACTCACAACC AATCACAATG 
    GTCACTGCTT ATGATTACCC TTCTGCTGTT CACCTCGATA TGGCCGCCAT TGATATCTGT 
    CTTGTTGGTG ATTCTGCTTC CATGGTTGTT CATGGTCATG ATACTACTTT GCCTATTACT 
    TTAGATGAAA TGCTTGTTCA TTGTCGTGCA GTTGCTCGTG GTGCTAAAAC TCCTCTTCTT 
    GTTGGTGATT TGCCTTTTGG AACCTATGAA TGTAGTTCTA ATCAGGCAGT GGACACGGCA 
    GTTCGAATTT TGAAAGAAGG ACAAATGGAT GCCATAAAGT TGGAAGGAGG TTCACCTTCA 
    AGAATTGTTG CAGCAAAAGC TATTGTTGAA GCTGGAATAG CTGTGATTGG GCATGTTGGT 
    CTTACTCCAC AAGCCATTAG TGTTTTAGGA GGATTTAGGC CTCAGGGTAG GAATGTTGCA 
    AGTGCTGTCA AGGTTGTCGA GACAGCATTG GCTTTGCAAG AAGCAGGGTG TTTTGCTGTT 
    GTTCTTGAAT GTGTGCCTGC ACCGGTGACT GCAGCAGCAA CAGCGGCGCT TCAAATTCCT 
    ACAATTGGAA TTGGGGCTGG TCCCTATTGC AGTGGACAGG TGCTTGTTTA CCATGATCTT 
    CTTGGTATGT TACAACACCC TCACCATGCA AAGGTGACTC CAAAATTTTG TAAACAGTAC 
    GCTCGTGTTG GAGACGTCAT CAATAAAGCC TTACTGGAAT ATAAGGAAGA TGTGATGAAT 
    GGTTCATTTC CTGATGCTCA ACATAGTCCA TACAAAATCA GTGAAACTGA TGCTAATGGT 
    TTCTTAAATG AGCTGCAAAA GTTAGGTTTT GACAAGGCTG CATCTGCGGC ATCTGAAGCA 
    GTTCAGAAGA TGGTTTCAAA ATCAACAAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
    BMC genomics15312(2014 Apr)
    Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD


    The Medicago genome provides insight into the evolution of rhizobial symbioses.
    Nature480(7378)520-4(2011 Nov)
    Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA


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