LOC11410021 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11410021
Entrez Gene ID	11410021
Full Name	FTSH extracellular protease family protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003590939.2	XP_003590987.2	FTSH extracellular protease family protein
XM_013613505.1	XP_013468959.1	FTSH extracellular protease family protein
XM_003590939.3	XP_003590987.2	probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X1
XM_013613505.2	XP_013468959.2	probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11410021 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11410021 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe212294	XM_013613505.1 Latest version!	Medicago truncatula FTSH extracellular protease family protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe207965	XM_003590939.3 Latest version!	Medicago truncatula probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic (LOC11410021), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe257970	XM_013613505.2 Latest version!	Medicago truncatula probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic (LOC11410021), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMe207965	XM_003590939.2	Medicago truncatula FTSH extracellular protease family protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11410021 ( XM_013613505.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468959.1 Medicago truncatula FTSH extracellular protease family protein mRNA.

Price & Availability↑

CloneID	OMe212294
Clone ID Related Accession (Same CDS sequence)	XM_013613505.1
Accession Version	XM_013613505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	FTSH extracellular protease family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAG TAAGAATTGA GTACTTTATG TTTGCATTAC ATAAAAAAGG GAAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468959.1
CDS	100..2919
Translation	MDSILYSSSSPPLRFPSHLPFTSFRPLPNHTLLFKPPRASITLSANQQNNHTVLSQPTNSTLVLSQCCLTKQLILRALFCFAVGVSTFGTFQIAPAFALPTIPWVQFLSKNKENKNQHEYSDCTQKVLDTVPSLLRTIEEVRKGNGDFEDVKRALEFVKLKKYEMEKEILERMHPVLMDLKEELRLLQIKEGEISWQMAEVNREHRKLMGWEMDMKDNVVNEVEKKVLDKRMVELEKKWNEILVKIDEMEDVISRKETVALSYGVLEICFIQRECENLVERFKQEIKQKKIGSSFASSVNKLSKSVIQEDLETVQRKQIEQTILPSIVDVDDLGPFFHQDSVDFAQHLERSLKDSREQQKNLEAQIRKDMQYDKEKRSVVYSPEEEERILLDRDRVVSKTWYNEEKNRWEMDPVAVPHAVSKKLIEHVRIRYDGRAMYIALKGEDKEFYVDIKEFERLFEYIGGFDVLYRKMLACGIPTAVHLMWIPLSELSVHQRISVILRFPLRFLSGRWNSETVLTTTNLIFDNIKEMTDDIMTVIGFPIVEYILPNPVRVKLGMAWPEEETMNTPWYLNWQLNAEARVQSRRADGDFRWIMLFIARAAISGFVLINVFQFMRRKIPRLLGYGPIQKNPNRRKLEQMAYYFDERKGRMRDRRREGVDPIKTAFEHMKRVKKPPIPLNNFSSIDSMKEEISEVVAFLQNPRAFQEMGARAPRGVLIVGERGTGKTSLAMAIAAEAKVPVVEIKAQQLEAGMWVGQSASNVRELFQTARDLAPVILFVEDFDLFAGVRGKFIHTENQDHEAFINQLLVELDGFEKQDGVVLMATTRNLKQIDEALQRPGRMDRIFHLQRPTQAERENILYSAAKETMDDQLVEYVDWKKVAEKTALLRPIELKLVPIALEGSAFRSKVLDTDEIMSYCSFFAVRIEYFMFALHKKGKC

Target ORF information:

RefSeq Version	XM_013613505.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula FTSH extracellular protease family protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613505.1

ORF Insert Sequence:

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAG TAAGAATTGA GTACTTTATG TTTGCATTAC ATAAAAAAGG GAAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11410021 ( XM_003590939.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003590987.2 Medicago truncatula FTSH extracellular protease family protein mRNA.

Price & Availability↑

CloneID	OMe207965
Clone ID Related Accession (Same CDS sequence)	XM_003590939.2 , XM_003590939.3
Accession Version	XM_003590939.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	FTSH extracellular protease family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). On Aug 25, 2015 this sequence version replaced XM_003590939.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAA CTTTCAGTTC TTCGATGCCC CAATGGCTGA GGCAAACCAA AATTGTCAAG 
AAGTTAAACA AAATGTTGGT GAATCACCTG GGACTGACAT TAACAAAAGA AGATCTCCAA 
AATGTTGTTG ATTTGATGGA ACCATACGGC CAGATAAGCA ATGGAATAGA GCTTTTGAGC 
CCTCCTCTTG ATTGGACAAG GGAAACTAAA TTTCCACATG CTGTCTGGGC TGCTGGTCGT 
GGTCTTGTTG CTCATTTATT ACCAAATTTT GATGTTGTTC ATAATCTTTG GCTTGAGCCT 
CTATCTTGGC AGGGAATTGG ATGTACGAAA ATCACGAAAG CAAGAAATGA TGGTTACATT 
GATGGGAATA TAGAATCAAG ATCATATCTT GAAAAGAAAC TTGTATTTTG TTTTGGTTCT 
TATGTTGCAT CCCAAATGTT ACTTCCTTTT GGGGAAGAAA ACTTACTGTC TTCATCTGAG 
ATGCAGCAGG CACAAGAGAT TGCTACGCGG ATGGTCATTC AATATGGATG GGGGCCTGAT 
GATAGTGCTG CAGTTTATTA CTGTAGTAAT GCGGTTGCTA CATTAAGCAT GGCAGGTGAC 
CATGAGTATG TGATGACAGC TAAAGTTCAA AAGATGTATG ATTTGGCATA TCTCAAAGCA 
AGAGAAATGC TGCAGAGAAA TCGCCTGGTA CTAGAAAAGA TTGTAGAGGA ATTACTTGAA 
TTTGAAATTT TGACTAGAAA GGATTTAGAA AGAATTACTG AAGCTAATGA TGGAATCAGG 
GAGAAAGAGC CCTTTACTCT TGTTGAAGTT CAAGCTAGTG AGCCAACGTC CGGCAGCCTC 
CTTGAAAGAG GAAATGCATC AGGAGGTGCT TTGCTAGCAT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003590987.2
CDS	100..3744
Translation	MDSILYSSSSPPLRFPSHLPFTSFRPLPNHTLLFKPPRASITLSANQQNNHTVLSQPTNSTLVLSQCCLTKQLILRALFCFAVGVSTFGTFQIAPAFALPTIPWVQFLSKNKENKNQHEYSDCTQKVLDTVPSLLRTIEEVRKGNGDFEDVKRALEFVKLKKYEMEKEILERMHPVLMDLKEELRLLQIKEGEISWQMAEVNREHRKLMGWEMDMKDNVVNEVEKKVLDKRMVELEKKWNEILVKIDEMEDVISRKETVALSYGVLEICFIQRECENLVERFKQEIKQKKIGSSFASSVNKLSKSVIQEDLETVQRKQIEQTILPSIVDVDDLGPFFHQDSVDFAQHLERSLKDSREQQKNLEAQIRKDMQYDKEKRSVVYSPEEEERILLDRDRVVSKTWYNEEKNRWEMDPVAVPHAVSKKLIEHVRIRYDGRAMYIALKGEDKEFYVDIKEFERLFEYIGGFDVLYRKMLACGIPTAVHLMWIPLSELSVHQRISVILRFPLRFLSGRWNSETVLTTTNLIFDNIKEMTDDIMTVIGFPIVEYILPNPVRVKLGMAWPEEETMNTPWYLNWQLNAEARVQSRRADGDFRWIMLFIARAAISGFVLINVFQFMRRKIPRLLGYGPIQKNPNRRKLEQMAYYFDERKGRMRDRRREGVDPIKTAFEHMKRVKKPPIPLNNFSSIDSMKEEISEVVAFLQNPRAFQEMGARAPRGVLIVGERGTGKTSLAMAIAAEAKVPVVEIKAQQLEAGMWVGQSASNVRELFQTARDLAPVILFVEDFDLFAGVRGKFIHTENQDHEAFINQLLVELDGFEKQDGVVLMATTRNLKQIDEALQRPGRMDRIFHLQRPTQAERENILYSAAKETMDDQLVEYVDWKKVAEKTALLRPIELKLVPIALEGSAFRSKVLDTDEIMSYCSFFATFSSSMPQWLRQTKIVKKLNKMLVNHLGLTLTKEDLQNVVDLMEPYGQISNGIELLSPPLDWTRETKFPHAVWAAGRGLVAHLLPNFDVVHNLWLEPLSWQGIGCTKITKARNDGYIDGNIESRSYLEKKLVFCFGSYVASQMLLPFGEENLLSSSEMQQAQEIATRMVIQYGWGPDDSAAVYYCSNAVATLSMAGDHEYVMTAKVQKMYDLAYLKAREMLQRNRLVLEKIVEELLEFEILTRKDLERITEANDGIREKEPFTLVEVQASEPTSGSLLERGNASGGALLAS

Target ORF information:

RefSeq Version	XM_003590939.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula FTSH extracellular protease family protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003590939.2

ORF Insert Sequence:

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAA CTTTCAGTTC TTCGATGCCC CAATGGCTGA GGCAAACCAA AATTGTCAAG 
AAGTTAAACA AAATGTTGGT GAATCACCTG GGACTGACAT TAACAAAAGA AGATCTCCAA 
AATGTTGTTG ATTTGATGGA ACCATACGGC CAGATAAGCA ATGGAATAGA GCTTTTGAGC 
CCTCCTCTTG ATTGGACAAG GGAAACTAAA TTTCCACATG CTGTCTGGGC TGCTGGTCGT 
GGTCTTGTTG CTCATTTATT ACCAAATTTT GATGTTGTTC ATAATCTTTG GCTTGAGCCT 
CTATCTTGGC AGGGAATTGG ATGTACGAAA ATCACGAAAG CAAGAAATGA TGGTTACATT 
GATGGGAATA TAGAATCAAG ATCATATCTT GAAAAGAAAC TTGTATTTTG TTTTGGTTCT 
TATGTTGCAT CCCAAATGTT ACTTCCTTTT GGGGAAGAAA ACTTACTGTC TTCATCTGAG 
ATGCAGCAGG CACAAGAGAT TGCTACGCGG ATGGTCATTC AATATGGATG GGGGCCTGAT 
GATAGTGCTG CAGTTTATTA CTGTAGTAAT GCGGTTGCTA CATTAAGCAT GGCAGGTGAC 
CATGAGTATG TGATGACAGC TAAAGTTCAA AAGATGTATG ATTTGGCATA TCTCAAAGCA 
AGAGAAATGC TGCAGAGAAA TCGCCTGGTA CTAGAAAAGA TTGTAGAGGA ATTACTTGAA 
TTTGAAATTT TGACTAGAAA GGATTTAGAA AGAATTACTG AAGCTAATGA TGGAATCAGG 
GAGAAAGAGC CCTTTACTCT TGTTGAAGTT CAAGCTAGTG AGCCAACGTC CGGCAGCCTC 
CTTGAAAGAG GAAATGCATC AGGAGGTGCT TTGCTAGCAT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11410021 ( XM_003590939.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003590987.2 Medicago truncatula probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic (LOC11410021), transcript variant X1, mRNA.

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CloneID	OMe207965
Clone ID Related Accession (Same CDS sequence)	XM_003590939.2 , XM_003590939.3
Accession Version	XM_003590939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003590939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAA CTTTCAGTTC TTCGATGCCC CAATGGCTGA GGCAAACCAA AATTGTCAAG 
AAGTTAAACA AAATGTTGGT GAATCACCTG GGACTGACAT TAACAAAAGA AGATCTCCAA 
AATGTTGTTG ATTTGATGGA ACCATACGGC CAGATAAGCA ATGGAATAGA GCTTTTGAGC 
CCTCCTCTTG ATTGGACAAG GGAAACTAAA TTTCCACATG CTGTCTGGGC TGCTGGTCGT 
GGTCTTGTTG CTCATTTATT ACCAAATTTT GATGTTGTTC ATAATCTTTG GCTTGAGCCT 
CTATCTTGGC AGGGAATTGG ATGTACGAAA ATCACGAAAG CAAGAAATGA TGGTTACATT 
GATGGGAATA TAGAATCAAG ATCATATCTT GAAAAGAAAC TTGTATTTTG TTTTGGTTCT 
TATGTTGCAT CCCAAATGTT ACTTCCTTTT GGGGAAGAAA ACTTACTGTC TTCATCTGAG 
ATGCAGCAGG CACAAGAGAT TGCTACGCGG ATGGTCATTC AATATGGATG GGGGCCTGAT 
GATAGTGCTG CAGTTTATTA CTGTAGTAAT GCGGTTGCTA CATTAAGCAT GGCAGGTGAC 
CATGAGTATG TGATGACAGC TAAAGTTCAA AAGATGTATG ATTTGGCATA TCTCAAAGCA 
AGAGAAATGC TGCAGAGAAA TCGCCTGGTA CTAGAAAAGA TTGTAGAGGA ATTACTTGAA 
TTTGAAATTT TGACTAGAAA GGATTTAGAA AGAATTACTG AAGCTAATGA TGGAATCAGG 
GAGAAAGAGC CCTTTACTCT TGTTGAAGTT CAAGCTAGTG AGCCAACGTC CGGCAGCCTC 
CTTGAAAGAG GAAATGCATC AGGAGGTGCT TTGCTAGCAT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003590987.2
CDS	118..3762
Translation	MDSILYSSSSPPLRFPSHLPFTSFRPLPNHTLLFKPPRASITLSANQQNNHTVLSQPTNSTLVLSQCCLTKQLILRALFCFAVGVSTFGTFQIAPAFALPTIPWVQFLSKNKENKNQHEYSDCTQKVLDTVPSLLRTIEEVRKGNGDFEDVKRALEFVKLKKYEMEKEILERMHPVLMDLKEELRLLQIKEGEISWQMAEVNREHRKLMGWEMDMKDNVVNEVEKKVLDKRMVELEKKWNEILVKIDEMEDVISRKETVALSYGVLEICFIQRECENLVERFKQEIKQKKIGSSFASSVNKLSKSVIQEDLETVQRKQIEQTILPSIVDVDDLGPFFHQDSVDFAQHLERSLKDSREQQKNLEAQIRKDMQYDKEKRSVVYSPEEEERILLDRDRVVSKTWYNEEKNRWEMDPVAVPHAVSKKLIEHVRIRYDGRAMYIALKGEDKEFYVDIKEFERLFEYIGGFDVLYRKMLACGIPTAVHLMWIPLSELSVHQRISVILRFPLRFLSGRWNSETVLTTTNLIFDNIKEMTDDIMTVIGFPIVEYILPNPVRVKLGMAWPEEETMNTPWYLNWQLNAEARVQSRRADGDFRWIMLFIARAAISGFVLINVFQFMRRKIPRLLGYGPIQKNPNRRKLEQMAYYFDERKGRMRDRRREGVDPIKTAFEHMKRVKKPPIPLNNFSSIDSMKEEISEVVAFLQNPRAFQEMGARAPRGVLIVGERGTGKTSLAMAIAAEAKVPVVEIKAQQLEAGMWVGQSASNVRELFQTARDLAPVILFVEDFDLFAGVRGKFIHTENQDHEAFINQLLVELDGFEKQDGVVLMATTRNLKQIDEALQRPGRMDRIFHLQRPTQAERENILYSAAKETMDDQLVEYVDWKKVAEKTALLRPIELKLVPIALEGSAFRSKVLDTDEIMSYCSFFATFSSSMPQWLRQTKIVKKLNKMLVNHLGLTLTKEDLQNVVDLMEPYGQISNGIELLSPPLDWTRETKFPHAVWAAGRGLVAHLLPNFDVVHNLWLEPLSWQGIGCTKITKARNDGYIDGNIESRSYLEKKLVFCFGSYVASQMLLPFGEENLLSSSEMQQAQEIATRMVIQYGWGPDDSAAVYYCSNAVATLSMAGDHEYVMTAKVQKMYDLAYLKAREMLQRNRLVLEKIVEELLEFEILTRKDLERITEANDGIREKEPFTLVEVQASEPTSGSLLERGNASGGALLAS

Target ORF information:

RefSeq Version	XM_003590939.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic (LOC11410021), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003590939.3

ORF Insert Sequence:

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAA CTTTCAGTTC TTCGATGCCC CAATGGCTGA GGCAAACCAA AATTGTCAAG 
AAGTTAAACA AAATGTTGGT GAATCACCTG GGACTGACAT TAACAAAAGA AGATCTCCAA 
AATGTTGTTG ATTTGATGGA ACCATACGGC CAGATAAGCA ATGGAATAGA GCTTTTGAGC 
CCTCCTCTTG ATTGGACAAG GGAAACTAAA TTTCCACATG CTGTCTGGGC TGCTGGTCGT 
GGTCTTGTTG CTCATTTATT ACCAAATTTT GATGTTGTTC ATAATCTTTG GCTTGAGCCT 
CTATCTTGGC AGGGAATTGG ATGTACGAAA ATCACGAAAG CAAGAAATGA TGGTTACATT 
GATGGGAATA TAGAATCAAG ATCATATCTT GAAAAGAAAC TTGTATTTTG TTTTGGTTCT 
TATGTTGCAT CCCAAATGTT ACTTCCTTTT GGGGAAGAAA ACTTACTGTC TTCATCTGAG 
ATGCAGCAGG CACAAGAGAT TGCTACGCGG ATGGTCATTC AATATGGATG GGGGCCTGAT 
GATAGTGCTG CAGTTTATTA CTGTAGTAAT GCGGTTGCTA CATTAAGCAT GGCAGGTGAC 
CATGAGTATG TGATGACAGC TAAAGTTCAA AAGATGTATG ATTTGGCATA TCTCAAAGCA 
AGAGAAATGC TGCAGAGAAA TCGCCTGGTA CTAGAAAAGA TTGTAGAGGA ATTACTTGAA 
TTTGAAATTT TGACTAGAAA GGATTTAGAA AGAATTACTG AAGCTAATGA TGGAATCAGG 
GAGAAAGAGC CCTTTACTCT TGTTGAAGTT CAAGCTAGTG AGCCAACGTC CGGCAGCCTC 
CTTGAAAGAG GAAATGCATC AGGAGGTGCT TTGCTAGCAT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11410021 ( XM_013613505.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468959.2 Medicago truncatula probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic (LOC11410021), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe257970
Clone ID Related Accession (Same CDS sequence)	XM_013613505.2
Accession Version	XM_013613505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013613505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAA CTTTCAGTTC TTCGATGCCC CAATGGCTGA GGCAAACCAA AATTGTCAAG 
AAGTTAAACA AAATGTTGGT GAATCACCTG GGACTGACAT TAACAAAAGA AGATCTCCAA 
AATGTTGTTG ATTTGATGGA ACCATACGGC CAGATAAGCA ATGGAATAGA GCTTTTGAGC 
CCTCCTCTTG ATTGGACAAG GGAAACTAAA TTTCCACATG CTGTCTGGGC TGCTGGTCGT 
GGTCTTGTTG CTCATTTATT ACCAAATTTT GATGTTGTTC ATAATCTTTG GCTTGAGCCT 
CTATCTTGGC AGGGAATTGG ATGTACGAAA ATCACGAAAG CAAGAAATGA TGGTTACATT 
GATGGGAATA TAGAATCAAG ATCATATCTT GAAAAGAAAC TTGTATTTTG TTTTGGTTCT 
TATGTTGCAT CCCAAATGTT ACTTCCTTTT GGGGAAGAAA ACTTACTGTC TTCATCTGAG 
ATGCAGCAGG CACAAGAGAT TGCTACGCGG ATGGTCATTC AATATGGATG GGGGCCTGAT 
GATAGTGCTG CAGTTTATTA CTGTAGTAAT GCGGTCCTAA CAATGGTCAA TGAAATAATG 
GAAGATATCT TTTTTTGGTG GTGGCCGGGG TTCGAACCTC AGACCTTGCA TATATTGTGC 
AGTCCATACC AACTGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468959.2
CDS	118..3558
Translation	MDSILYSSSSPPLRFPSHLPFTSFRPLPNHTLLFKPPRASITLSANQQNNHTVLSQPTNSTLVLSQCCLTKQLILRALFCFAVGVSTFGTFQIAPAFALPTIPWVQFLSKNKENKNQHEYSDCTQKVLDTVPSLLRTIEEVRKGNGDFEDVKRALEFVKLKKYEMEKEILERMHPVLMDLKEELRLLQIKEGEISWQMAEVNREHRKLMGWEMDMKDNVVNEVEKKVLDKRMVELEKKWNEILVKIDEMEDVISRKETVALSYGVLEICFIQRECENLVERFKQEIKQKKIGSSFASSVNKLSKSVIQEDLETVQRKQIEQTILPSIVDVDDLGPFFHQDSVDFAQHLERSLKDSREQQKNLEAQIRKDMQYDKEKRSVVYSPEEEERILLDRDRVVSKTWYNEEKNRWEMDPVAVPHAVSKKLIEHVRIRYDGRAMYIALKGEDKEFYVDIKEFERLFEYIGGFDVLYRKMLACGIPTAVHLMWIPLSELSVHQRISVILRFPLRFLSGRWNSETVLTTTNLIFDNIKEMTDDIMTVIGFPIVEYILPNPVRVKLGMAWPEEETMNTPWYLNWQLNAEARVQSRRADGDFRWIMLFIARAAISGFVLINVFQFMRRKIPRLLGYGPIQKNPNRRKLEQMAYYFDERKGRMRDRRREGVDPIKTAFEHMKRVKKPPIPLNNFSSIDSMKEEISEVVAFLQNPRAFQEMGARAPRGVLIVGERGTGKTSLAMAIAAEAKVPVVEIKAQQLEAGMWVGQSASNVRELFQTARDLAPVILFVEDFDLFAGVRGKFIHTENQDHEAFINQLLVELDGFEKQDGVVLMATTRNLKQIDEALQRPGRMDRIFHLQRPTQAERENILYSAAKETMDDQLVEYVDWKKVAEKTALLRPIELKLVPIALEGSAFRSKVLDTDEIMSYCSFFATFSSSMPQWLRQTKIVKKLNKMLVNHLGLTLTKEDLQNVVDLMEPYGQISNGIELLSPPLDWTRETKFPHAVWAAGRGLVAHLLPNFDVVHNLWLEPLSWQGIGCTKITKARNDGYIDGNIESRSYLEKKLVFCFGSYVASQMLLPFGEENLLSSSEMQQAQEIATRMVIQYGWGPDDSAAVYYCSNAVLTMVNEIMEDIFFWWWPGFEPQTLHILCSPYQLS

Target ORF information:

RefSeq Version	XM_013613505.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic (LOC11410021), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613505.2

ORF Insert Sequence:

ATGGATTCCA TTCTCTATTC TTCTTCTTCA CCACCTCTTC GATTTCCATC ACACCTTCCT 
TTCACCTCTT TCCGCCCCCT TCCAAATCAT ACACTCCTCT TCAAACCACC ACGCGCTTCC 
ATCACTCTCT CCGCAAATCA ACAAAACAAC CACACCGTTT TATCCCAACC AACAAACTCA 
ACTCTTGTTT TATCCCAATG CTGCTTAACA AAACAACTCA TTCTTCGCGC TTTATTCTGT 
TTCGCCGTCG GTGTTTCCAC CTTCGGAACA TTCCAAATTG CGCCCGCGTT TGCACTTCCG 
ACGATACCTT GGGTTCAATT TTTGAGTAAG AACAAAGAGA ACAAGAATCA ACACGAGTAT 
TCTGATTGCA CGCAAAAAGT GTTGGATACT GTGCCGAGTT TGTTGAGGAC TATTGAAGAG 
GTTAGGAAAG GGAACGGTGA TTTTGAAGAT GTGAAACGAG CTTTGGAGTT TGTGAAATTG 
AAGAAATATG AAATGGAGAA AGAGATTCTC GAGAGGATGC ATCCGGTTTT GATGGATTTG 
AAGGAGGAGT TGAGGTTGTT GCAGATAAAG GAAGGTGAGA TTAGTTGGCA GATGGCGGAA 
GTGAACAGGG AGCATAGAAA ATTGATGGGC TGGGAGATGG ATATGAAGGA TAATGTAGTG 
AATGAGGTGG AGAAGAAGGT GTTGGACAAG AGAATGGTGG AGTTGGAGAA GAAATGGAAT 
GAGATTTTAG TGAAAATTGA TGAAATGGAG GATGTCATTT CGAGGAAAGA GACGGTGGCG 
TTGAGTTATG GGGTGTTGGA GATTTGTTTT ATCCAGAGGG AATGTGAGAA CTTGGTGGAG 
AGATTTAAAC AAGAAATCAA ACAGAAGAAA ATCGGAAGTT CATTCGCAAG CTCAGTTAAC 
AAGCTATCAA AATCCGTCAT TCAAGAAGAC TTGGAAACTG TGCAAAGAAA GCAAATAGAG 
CAAACTATTC TTCCTAGCAT TGTGGATGTT GATGATCTCG GGCCATTCTT TCATCAAGAC 
TCTGTTGATT TTGCCCAACA TTTAGAAAGA AGTCTCAAAG ACTCAAGGGA GCAGCAGAAG 
AATTTAGAGG CCCAGATAAG GAAAGATATG CAGTATGACA AGGAAAAGCG TAGTGTTGTG 
TACTCACCTG AAGAAGAGGA AAGGATTCTT TTGGACCGAG ATAGGGTAGT GTCAAAGACT 
TGGTACAATG AAGAGAAAAA TAGATGGGAG ATGGATCCAG TGGCTGTTCC TCATGCTGTC 
TCAAAAAAGC TAATAGAGCA TGTTCGGATT AGGTATGATG GGCGTGCAAT GTATATTGCA 
TTGAAGGGGG AGGACAAAGA GTTTTATGTA GACATAAAGG AATTCGAAAG GCTTTTTGAA 
TATATTGGGG GTTTTGATGT GCTATATAGG AAAATGCTAG CCTGTGGTAT TCCAACAGCT 
GTTCATCTAA TGTGGATTCC GTTGTCAGAG TTAAGTGTGC ACCAACGGAT TTCTGTGATA 
TTAAGGTTCC CTCTTCGATT TTTGAGTGGC CGGTGGAATT CCGAAACTGT CTTGACTACA 
ACAAATTTGA TCTTTGACAA TATTAAGGAA ATGACTGATG ACATAATGAC AGTGATAGGG 
TTTCCTATTG TGGAATATAT ACTCCCTAAC CCGGTGAGGG TAAAATTGGG AATGGCTTGG 
CCAGAGGAAG AGACAATGAA CACTCCATGG TACTTGAACT GGCAATTAAA TGCAGAAGCA 
AGAGTTCAAT CCAGACGAGC AGATGGTGAT TTTCGATGGA TCATGTTGTT TATCGCAAGA 
GCTGCTATTT CAGGATTTGT TTTGATTAAC GTGTTCCAAT TTATGAGGAG AAAAATTCCA 
AGACTTCTTG GTTATGGTCC TATCCAAAAA AATCCGAATA GGAGGAAACT TGAGCAAATG 
GCTTATTACT TTGATGAAAG AAAGGGACGT ATGAGGGATA GGAGAAGGGA GGGTGTTGAT 
CCTATAAAAA CAGCTTTTGA GCACATGAAG AGGGTGAAGA AACCACCAAT ACCATTGAAT 
AACTTTTCTA GCATTGACTC TATGAAAGAG GAAATCAGTG AAGTTGTGGC ATTTTTGCAA 
AATCCAAGGG CATTTCAAGA AATGGGAGCG CGGGCACCTC GGGGTGTTCT CATTGTAGGT 
GAGAGGGGTA CGGGCAAAAC GTCTCTAGCA ATGGCTATAG CTGCTGAGGC TAAGGTGCCT 
GTTGTTGAAA TTAAGGCCCA ACAATTGGAG GCTGGGATGT GGGTTGGACA AAGTGCATCC 
AATGTTCGTG AATTGTTTCA AACAGCAAGA GATTTGGCTC CTGTAATATT GTTCGTGGAA 
GATTTTGACC TATTTGCTGG TGTACGTGGC AAATTTATCC ACACCGAAAA TCAAGATCAT 
GAAGCTTTCA TTAATCAATT GCTTGTGGAA CTTGACGGAT TTGAGAAACA AGATGGTGTT 
GTTTTGATGG CTACTACAAG AAATCTTAAG CAAATTGACG AGGCCTTGCA GAGGCCAGGT 
CGCATGGATA GAATATTTCA TCTTCAAAGA CCAACACAGG CAGAGAGAGA AAACATTTTG 
TACTCAGCAG CGAAGGAAAC CATGGATGAC CAGCTTGTCG AATATGTAGA CTGGAAAAAG 
GTTGCTGAGA AGACGGCTCT TTTACGACCT ATCGAGTTAA AACTTGTTCC CATAGCTTTA 
GAAGGAAGTG CCTTCCGGAG CAAAGTTCTG GACACAGATG AAATAATGAG CTACTGTAGT 
TTCTTTGCAA CTTTCAGTTC TTCGATGCCC CAATGGCTGA GGCAAACCAA AATTGTCAAG 
AAGTTAAACA AAATGTTGGT GAATCACCTG GGACTGACAT TAACAAAAGA AGATCTCCAA 
AATGTTGTTG ATTTGATGGA ACCATACGGC CAGATAAGCA ATGGAATAGA GCTTTTGAGC 
CCTCCTCTTG ATTGGACAAG GGAAACTAAA TTTCCACATG CTGTCTGGGC TGCTGGTCGT 
GGTCTTGTTG CTCATTTATT ACCAAATTTT GATGTTGTTC ATAATCTTTG GCTTGAGCCT 
CTATCTTGGC AGGGAATTGG ATGTACGAAA ATCACGAAAG CAAGAAATGA TGGTTACATT 
GATGGGAATA TAGAATCAAG ATCATATCTT GAAAAGAAAC TTGTATTTTG TTTTGGTTCT 
TATGTTGCAT CCCAAATGTT ACTTCCTTTT GGGGAAGAAA ACTTACTGTC TTCATCTGAG 
ATGCAGCAGG CACAAGAGAT TGCTACGCGG ATGGTCATTC AATATGGATG GGGGCCTGAT 
GATAGTGCTG CAGTTTATTA CTGTAGTAAT GCGGTCCTAA CAATGGTCAA TGAAATAATG 
GAAGATATCT TTTTTTGGTG GTGGCCGGGG TTCGAACCTC AGACCTTGCA TATATTGTGC 
AGTCCATACC AACTGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11410021 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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