aff3 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	aff3
Entrez Gene ID	101482124
Full Name	AF4/FMR2 family member 3
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004551261.4	XP_004551318.2	AF4/FMR2 family member 3
XM_004551261.3	XP_004551318.1	AF4/FMR2 family member 3

Pathways from BioSystems
Gene Ontology

The following aff3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aff3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMa11036	XM_004551261.3 Latest version!	Maylandia zebra AF4/FMR2 family member 3 (aff3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMa363980	XM_004551261.4 Latest version!	Maylandia zebra AF4/FMR2 family member 3 (aff3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aff3 ( XM_004551261.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004551318.1 Maylandia zebra AF4/FMR2 family member 3 (aff3), mRNA.

Price & Availability↑

CloneID	OMa11036
Clone ID Related Accession (Same CDS sequence)	XM_004551261.3
Accession Version	XM_004551261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	AF4/FMR2 family member 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445462.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004551261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACTG TCCTCGCTGG CAAAGGGAAA CTTTCCATTG TCTGCTTTCT GCTCCGATGC 
GCATATTCTC AGGAAAGAGG AAGCCAACAC AACAGCTGTG CCAAAGATAT ATTTGAGGAC 
ATGACCCAGT CCTGGAGGGA CTTTGGGGAC TCCTTGAGGC GTGACCCAGC ACAACGAATC 
CTGTACAACT CTAAGGATAC CAAACAGCCA ATTACACAAC AAAGGCAAAA CCGAGGGGAT 
GTTCCGCTGC GAATGGCCCG TCACCCTCAT GTGGCCCCCC ACAAATCCAT GCTCGCCGAT 
GACTTGAAGC TGAGCAGTGA TGATGAGGAA ACTCCCACGG TGATTCATGA GTCAGCTTCC 
TGGGACAGAC ACAGCCTGTC AGCACAGCGA GCGCACACAC ATGGCAGGCG GGTGAGACAT 
TCCAGCTCAG ACTCCTCAGG CTCAGACTCC ACCAATGAGT CAGGCAGCAG TAGCTTTCAT 
TCCCGGAGCC CAAGCCGGAG TCCAGAAGTT CACCCTGATC CCGAGTCGCC TGCCAACATC 
CAGCCACTGT GCAACAACAA GGAGCCTGAT CACCCCCCTG CTGCCCAGTG GCAGTTAGAT 
AAATGGCTGA AGAAGTCGCG GAAAAAATGT GCCAGCACTG AGCAAAATCC CTCCCAGACT 
ATTCCAGGAC GCCTGCACTC TCCCCACACC CACCGAGCCC CATCCCCAGC AAGGTCATTT 
GACAGCAATC AGGAATACAG CCCTAGCCAA AGCCCTATTC CCAGCCCACA GTTCAGTTAC 
AGCAACAACA ACAGCCCCCT TCCAAGCCCT GGTTACAGCT ACTGTCCAAG TCCGAGCCCA 
TTTACCAGCA CTTGTCAAAG TCCCAGCCCT AGTCCAAGGC ACAGCCCAAT TCCAAGTCCA 
GTTCTAAGTA TTTGCCCCAG TCCATGTGGG ACACTAAGAG ACAGCCGTAG TCCTAGCCCT 
ATAGCTAGAG ATCCTCCAAG AAGCCCGAGT CCCAGTATAC CTGCTCCTTC CAGGGTTTGT 
CACTACCCTG AGTTTCAGAG TCAGAATCAA CCTCAACCCA GTCAATCAAC CAATTCCCAC 
AGGATAAAAA TTAGGCCATG GATCGCCCCG GTGTCTGACA CTAATACAAA TATCAAGTCC 
ACGAGTAATA CTCATCTTCA GCCATCTCAC AAACACAGAC CCAAGACTCG ACCACCACAG 
GACCAAGGCC AAAGTCAGAC TAAATCAAAG GCTTCTGTCA TTTTAACCTC TAGTCAGAAT 
CAAAGCCACA CATCTAGACC CAAGCCTAAC TTTTATCCAA GCTCTAAACA GCTGGCTGAT 
GTGTCCCAAA CTAGAAACAC ATCACAGCTT GCGCAAATCC AGGGAAGCAG GTCCAGTCAC 
AAGCCCCGAC CTCCATTTCA ACCAAATTCT CAACATAGCC CCACAAGAGC AAAGCATTTA 
GTCCCTACTG CTCGTGAAAT CAATCCTCTT CATAATTCTC AATCTACCTC CAGAGCTGCT 
AGTAATCCTG GTTGTGGGCC AAACTCTCAG TCCATCCCTG GCCTCAAAAA ACACAGGTCC 
AAGTCTTGGG AGGCCACTGC CCCCACCACT GTGAATGTTA ATCACGCGAA AACAACACCG 
AAAAAACCTC AAACTAAGAA ACAGGAGGTG GAACGCAGAA GAGATCATCT GACCACAGGA 
GAGGGGAGAA AACACGAGAG AAAGGAAGAT AGACAAAAGG AAAAACAGCA AGGGAAACGG 
GAGCAAAAGG AAGACAGGAG GCTGGCGGAG GAGCAGCTAT TGAGACGGTC CTGGATCCAG 
AGTTCGGCTG AAGAAGAGGA GGGGGAAGAG GAGGAAGGAG TGACGGAACA GGACAGGAGG 
AGAGAGGAGA CGGCAAGAGA GGAAAGTCGG AGGAAGAGGG AGGCGCAACA ACAGCGACAT 
AAATGGGAGG CCAAACATAA AGTCCCTACC AACCACTCAC ATCAGCAAGG GGGGACAAAA 
AAGCGGGGAA GAAGTGAGGA GGAGAGACAG TTCCAGCCAG ACCCGTCGCC TCCTTCATCT 
TCACGGTCCC CAACTCCTCA GAGAACATCC TCCTCATCCT CTTCTTCGTC CTCATCGAGT 
TCAGATTCTG AATCTGAATT TCAAGCTCCG ATTACCAAAG TCCCAGCAGA CTCCACCTCT 
CAGAGGAAAC TCGCCAAGCC AGGGCAACAG AGCCCAGGAA GGCCTGACGC CAGCAGGCCT 
AAGGTGGTGC AGCAGAGACA ACCGAGCTCA GGTAACCCGA GTGAGGACCA GCAAAGTGCA 
GGGAAGCAAA AACTCTACAC CCTCGTCCCC TTTGGACGAG GGAACAAGGC TGCACCCCAC 
TCCCAGCGAG GGCTCCGAAA TCTGGTGGTG CACATAGACC TCTGTCTTCT CAAGAGGGTC 
CCAGACACTG CTACTCAATC CACTCCTAAA AAAACCTCCT CTTCCTCCTC ATCCTTATCA 
GCCAAGGACA AGCAAATTGA GACTATGAAA CACCTGCACG TCCCTGAGAC ACAGACTAAA 
GACAGCACAA GAAAGCGCAA GCTGGAGAAT GGTGTGTCAC ACAGAGAAAA CAAGAGGAGT 
TATGCAGACA AAACCTCAGG CCACACAGAG TCGTCATCTT TCACAGCTGA GACCGTAACG 
ACTGATACCG TGGACAGTGG GTGTGTGGAG GACGATTTGG ACAGTAAGAG GCCGCTGTCT 
CCGCTGTCTC CCTTGTCTCC CCGGCCCCAG AGCCCCGAGA TCCCCAAGTC TAACTCCAAA 
ACAAAAGCTG CAGAACAGCA TCACACCAAC AAGAACAAAG ACAAGAACAG AGATTCTGCG 
ATAAAGCCCA AGATGGAAGT GGAGTGTGTG AAAGTGTCGA AGCAGCCCCA GCCACCACCT 
GAGTCCTGGA GCACTGCAGC ACAAAGGGGG ACTGTGCCAA ACCACGAAAC TCCTCACCAT 
GCTGAGTACT ACTTACATGA AGCCAAAAGG ATGAAGCACC GTGCTGATGC CATGGTCGAC 
AAGCTAGGGA AGGCTGTGAA CTATGTTGAT GCTGCTCTCT CGTTCATGGA ATGTGGGAAG 
GCAATGGAGG AAGGGCCGCT CGAAGCAAAG TCTCCTTATA CCATGTATTC GGAAACAGTG 
GAGCTCATTA GGTACGCAAT GAAGCTGAAG AGCCACTCTG GCCCCGGGGC GAGACAGGAA 
GACAAACAGC TGGCGGTTCT GTGTTTCCGA TGCCTGGCCC TCCTTTACTG GCAGATGTTT 
CGCTTGAAGA AGGATCACGC GCTGAAATAC TCCAAAGTGC TGCTGGATTA CTTTAAGAGC 
TCTCCTAAAG TGCCTACTAC CCCACCCGGT TGGAGTGACT CTGGGAAGGG TACAGGAGGA 
CCCCCCTCTT CACTCTCGCC TAATACCAAA CACCTCGCAC GGGTTTCCCA CGAGGGCAGC 
AGCTCTGCCT CCCTCATAAG CATTCCCCAC CGTATCCACC AGATGGCAGC AACTCACCTG 
AACATTACCA ACAGTGTGCT GTACAGCTAT GAGTACTGGG AGGTGGCAGA CAACCTTGCA 
AAGGAGAATA AAGAGTTCTT CAACTACTTG AATACTTTGT CTGGGCCATT GACTCTTCAC 
AGTAGCATCG CTCACGCTGT CCAGTACACC AGGCAGGCCC TTCAGTGGAT ACGCATTAGT 
GCCAAACTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004551318.1
CDS	171..3845
Translation	MPTVLAGKGKLSIVCFLLRCAYSQERGSQHNSCAKDIFEDMTQSWRDFGDSLRRDPAQRILYNSKDTKQPITQQRQNRGDVPLRMARHPHVAPHKSMLADDLKLSSDDEETPTVIHESASWDRHSLSAQRAHTHGRRVRHSSSDSSGSDSTNESGSSSFHSRSPSRSPEVHPDPESPANIQPLCNNKEPDHPPAAQWQLDKWLKKSRKKCASTEQNPSQTIPGRLHSPHTHRAPSPARSFDSNQEYSPSQSPIPSPQFSYSNNNSPLPSPGYSYCPSPSPFTSTCQSPSPSPRHSPIPSPVLSICPSPCGTLRDSRSPSPIARDPPRSPSPSIPAPSRVCHYPEFQSQNQPQPSQSTNSHRIKIRPWIAPVSDTNTNIKSTSNTHLQPSHKHRPKTRPPQDQGQSQTKSKASVILTSSQNQSHTSRPKPNFYPSSKQLADVSQTRNTSQLAQIQGSRSSHKPRPPFQPNSQHSPTRAKHLVPTAREINPLHNSQSTSRAASNPGCGPNSQSIPGLKKHRSKSWEATAPTTVNVNHAKTTPKKPQTKKQEVERRRDHLTTGEGRKHERKEDRQKEKQQGKREQKEDRRLAEEQLLRRSWIQSSAEEEEGEEEEGVTEQDRRREETAREESRRKREAQQQRHKWEAKHKVPTNHSHQQGGTKKRGRSEEERQFQPDPSPPSSSRSPTPQRTSSSSSSSSSSSSDSESEFQAPITKVPADSTSQRKLAKPGQQSPGRPDASRPKVVQQRQPSSGNPSEDQQSAGKQKLYTLVPFGRGNKAAPHSQRGLRNLVVHIDLCLLKRVPDTATQSTPKKTSSSSSSLSAKDKQIETMKHLHVPETQTKDSTRKRKLENGVSHRENKRSYADKTSGHTESSSFTAETVTTDTVDSGCVEDDLDSKRPLSPLSPLSPRPQSPEIPKSNSKTKAAEQHHTNKNKDKNRDSAIKPKMEVECVKVSKQPQPPPESWSTAAQRGTVPNHETPHHAEYYLHEAKRMKHRADAMVDKLGKAVNYVDAALSFMECGKAMEEGPLEAKSPYTMYSETVELIRYAMKLKSHSGPGARQEDKQLAVLCFRCLALLYWQMFRLKKDHALKYSKVLLDYFKSSPKVPTTPPGWSDSGKGTGGPPSSLSPNTKHLARVSHEGSSSASLISIPHRIHQMAATHLNITNSVLYSYEYWEVADNLAKENKEFFNYLNTLSGPLTLHSSIAHAVQYTRQALQWIRISAKLN

Target ORF information:

RefSeq Version	XM_004551261.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra AF4/FMR2 family member 3 (aff3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004551261.3

ORF Insert Sequence:

ATGCCTACTG TCCTCGCTGG CAAAGGGAAA CTTTCCATTG TCTGCTTTCT GCTCCGATGC 
GCATATTCTC AGGAAAGAGG AAGCCAACAC AACAGCTGTG CCAAAGATAT ATTTGAGGAC 
ATGACCCAGT CCTGGAGGGA CTTTGGGGAC TCCTTGAGGC GTGACCCAGC ACAACGAATC 
CTGTACAACT CTAAGGATAC CAAACAGCCA ATTACACAAC AAAGGCAAAA CCGAGGGGAT 
GTTCCGCTGC GAATGGCCCG TCACCCTCAT GTGGCCCCCC ACAAATCCAT GCTCGCCGAT 
GACTTGAAGC TGAGCAGTGA TGATGAGGAA ACTCCCACGG TGATTCATGA GTCAGCTTCC 
TGGGACAGAC ACAGCCTGTC AGCACAGCGA GCGCACACAC ATGGCAGGCG GGTGAGACAT 
TCCAGCTCAG ACTCCTCAGG CTCAGACTCC ACCAATGAGT CAGGCAGCAG TAGCTTTCAT 
TCCCGGAGCC CAAGCCGGAG TCCAGAAGTT CACCCTGATC CCGAGTCGCC TGCCAACATC 
CAGCCACTGT GCAACAACAA GGAGCCTGAT CACCCCCCTG CTGCCCAGTG GCAGTTAGAT 
AAATGGCTGA AGAAGTCGCG GAAAAAATGT GCCAGCACTG AGCAAAATCC CTCCCAGACT 
ATTCCAGGAC GCCTGCACTC TCCCCACACC CACCGAGCCC CATCCCCAGC AAGGTCATTT 
GACAGCAATC AGGAATACAG CCCTAGCCAA AGCCCTATTC CCAGCCCACA GTTCAGTTAC 
AGCAACAACA ACAGCCCCCT TCCAAGCCCT GGTTACAGCT ACTGTCCAAG TCCGAGCCCA 
TTTACCAGCA CTTGTCAAAG TCCCAGCCCT AGTCCAAGGC ACAGCCCAAT TCCAAGTCCA 
GTTCTAAGTA TTTGCCCCAG TCCATGTGGG ACACTAAGAG ACAGCCGTAG TCCTAGCCCT 
ATAGCTAGAG ATCCTCCAAG AAGCCCGAGT CCCAGTATAC CTGCTCCTTC CAGGGTTTGT 
CACTACCCTG AGTTTCAGAG TCAGAATCAA CCTCAACCCA GTCAATCAAC CAATTCCCAC 
AGGATAAAAA TTAGGCCATG GATCGCCCCG GTGTCTGACA CTAATACAAA TATCAAGTCC 
ACGAGTAATA CTCATCTTCA GCCATCTCAC AAACACAGAC CCAAGACTCG ACCACCACAG 
GACCAAGGCC AAAGTCAGAC TAAATCAAAG GCTTCTGTCA TTTTAACCTC TAGTCAGAAT 
CAAAGCCACA CATCTAGACC CAAGCCTAAC TTTTATCCAA GCTCTAAACA GCTGGCTGAT 
GTGTCCCAAA CTAGAAACAC ATCACAGCTT GCGCAAATCC AGGGAAGCAG GTCCAGTCAC 
AAGCCCCGAC CTCCATTTCA ACCAAATTCT CAACATAGCC CCACAAGAGC AAAGCATTTA 
GTCCCTACTG CTCGTGAAAT CAATCCTCTT CATAATTCTC AATCTACCTC CAGAGCTGCT 
AGTAATCCTG GTTGTGGGCC AAACTCTCAG TCCATCCCTG GCCTCAAAAA ACACAGGTCC 
AAGTCTTGGG AGGCCACTGC CCCCACCACT GTGAATGTTA ATCACGCGAA AACAACACCG 
AAAAAACCTC AAACTAAGAA ACAGGAGGTG GAACGCAGAA GAGATCATCT GACCACAGGA 
GAGGGGAGAA AACACGAGAG AAAGGAAGAT AGACAAAAGG AAAAACAGCA AGGGAAACGG 
GAGCAAAAGG AAGACAGGAG GCTGGCGGAG GAGCAGCTAT TGAGACGGTC CTGGATCCAG 
AGTTCGGCTG AAGAAGAGGA GGGGGAAGAG GAGGAAGGAG TGACGGAACA GGACAGGAGG 
AGAGAGGAGA CGGCAAGAGA GGAAAGTCGG AGGAAGAGGG AGGCGCAACA ACAGCGACAT 
AAATGGGAGG CCAAACATAA AGTCCCTACC AACCACTCAC ATCAGCAAGG GGGGACAAAA 
AAGCGGGGAA GAAGTGAGGA GGAGAGACAG TTCCAGCCAG ACCCGTCGCC TCCTTCATCT 
TCACGGTCCC CAACTCCTCA GAGAACATCC TCCTCATCCT CTTCTTCGTC CTCATCGAGT 
TCAGATTCTG AATCTGAATT TCAAGCTCCG ATTACCAAAG TCCCAGCAGA CTCCACCTCT 
CAGAGGAAAC TCGCCAAGCC AGGGCAACAG AGCCCAGGAA GGCCTGACGC CAGCAGGCCT 
AAGGTGGTGC AGCAGAGACA ACCGAGCTCA GGTAACCCGA GTGAGGACCA GCAAAGTGCA 
GGGAAGCAAA AACTCTACAC CCTCGTCCCC TTTGGACGAG GGAACAAGGC TGCACCCCAC 
TCCCAGCGAG GGCTCCGAAA TCTGGTGGTG CACATAGACC TCTGTCTTCT CAAGAGGGTC 
CCAGACACTG CTACTCAATC CACTCCTAAA AAAACCTCCT CTTCCTCCTC ATCCTTATCA 
GCCAAGGACA AGCAAATTGA GACTATGAAA CACCTGCACG TCCCTGAGAC ACAGACTAAA 
GACAGCACAA GAAAGCGCAA GCTGGAGAAT GGTGTGTCAC ACAGAGAAAA CAAGAGGAGT 
TATGCAGACA AAACCTCAGG CCACACAGAG TCGTCATCTT TCACAGCTGA GACCGTAACG 
ACTGATACCG TGGACAGTGG GTGTGTGGAG GACGATTTGG ACAGTAAGAG GCCGCTGTCT 
CCGCTGTCTC CCTTGTCTCC CCGGCCCCAG AGCCCCGAGA TCCCCAAGTC TAACTCCAAA 
ACAAAAGCTG CAGAACAGCA TCACACCAAC AAGAACAAAG ACAAGAACAG AGATTCTGCG 
ATAAAGCCCA AGATGGAAGT GGAGTGTGTG AAAGTGTCGA AGCAGCCCCA GCCACCACCT 
GAGTCCTGGA GCACTGCAGC ACAAAGGGGG ACTGTGCCAA ACCACGAAAC TCCTCACCAT 
GCTGAGTACT ACTTACATGA AGCCAAAAGG ATGAAGCACC GTGCTGATGC CATGGTCGAC 
AAGCTAGGGA AGGCTGTGAA CTATGTTGAT GCTGCTCTCT CGTTCATGGA ATGTGGGAAG 
GCAATGGAGG AAGGGCCGCT CGAAGCAAAG TCTCCTTATA CCATGTATTC GGAAACAGTG 
GAGCTCATTA GGTACGCAAT GAAGCTGAAG AGCCACTCTG GCCCCGGGGC GAGACAGGAA 
GACAAACAGC TGGCGGTTCT GTGTTTCCGA TGCCTGGCCC TCCTTTACTG GCAGATGTTT 
CGCTTGAAGA AGGATCACGC GCTGAAATAC TCCAAAGTGC TGCTGGATTA CTTTAAGAGC 
TCTCCTAAAG TGCCTACTAC CCCACCCGGT TGGAGTGACT CTGGGAAGGG TACAGGAGGA 
CCCCCCTCTT CACTCTCGCC TAATACCAAA CACCTCGCAC GGGTTTCCCA CGAGGGCAGC 
AGCTCTGCCT CCCTCATAAG CATTCCCCAC CGTATCCACC AGATGGCAGC AACTCACCTG 
AACATTACCA ACAGTGTGCT GTACAGCTAT GAGTACTGGG AGGTGGCAGA CAACCTTGCA 
AAGGAGAATA AAGAGTTCTT CAACTACTTG AATACTTTGT CTGGGCCATT GACTCTTCAC 
AGTAGCATCG CTCACGCTGT CCAGTACACC AGGCAGGCCC TTCAGTGGAT ACGCATTAGT 
GCCAAACTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aff3 ( XM_004551261.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004551318.2 Maylandia zebra AF4/FMR2 family member 3 (aff3), mRNA.

Price & Availability↑

CloneID	OMa363980
Clone ID Related Accession (Same CDS sequence)	XM_004551261.4
Accession Version	XM_004551261.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	AF4/FMR2 family member 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036795.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004551261.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACTG TCCTCGCTGG CAAAGGGAAA CTTTCCATTG TCTGCTTTCT GCTCCGATGC 
GCATATTCTC AGGAAAGAGG AAGCCAACAC AACAGCTGTG CCAAAGATAT ATTTGAGGAC 
ATGACCCAGT CCTGGAGGGA CTTTGGGGAC TCCTTGAGGC GTGACCCAGC ACAACGAATC 
CTGTACAACT CTAAGGATAC CAAACAGCCA ATTACACAAC AAAGGCAAAA CCGAGGGGAT 
GTTCCGCTGC GAATGGCCCG TCACCCTCAT GTGGCCCCCC ACAAATCCAT GCTCGCCGAT 
GACTTGAAGC TGAGCAGTGA TGATGAGGAA ACTCCCACGG TGATTCATGA GTCAGCTTCC 
TGGGACAGAC ACAGCCTGTC AGCACAGCGA GCGCACACAC ATGGCAGGCG GGTGAGACAT 
TCCAGCTCAG ACTCCTCAGG CTCAGACTCC ACCAATGAGT CAGGCAGCAG TAGCTTTCAT 
TCCCGGAGCC CAAGCCGGAG TCCAGAAGTT CACCCTGATC CCGAGTCGCC TGCCAACATC 
CAGCCACTGT GCAACAACAA GGAGCCTGAT CACCCCCCTG CTGCCCAGTG GCAGTTAGAT 
AAATGGCTGA AGAAGTCGCG GAAAAAATGT GCCAGCACTG AGCAAAATCC CTCCCAGACT 
ATTCCAGGAC GCCTGCACTC TCCCCACACC CACCGAGCCC CATCCCCAGC AAGGTCATTT 
GACAGCAATC AGGAATACAG CCCTAGCCAA AGCCCTATTC CCAGCCCACA GTTCAGTTAC 
AGCAACAACA ACAGCCCCCT TCCAAGCCCT GGTTACAGCT ACTGTCCAAG TCCGAGCCCA 
TTTACCAGCA CTTGTCAAAG TCCCAGCCCT AGTCCAAGGC ACAGCCCAAT TCCAAGTCCA 
GTTCTAAGTA TTTGCCCCAG TCCATGTGGG ACACTAAGAG ACAGCCGTAG TCCTAGCCCT 
ATAGCTAGAG ATCCTCCAAG AAGCCCGAGT CCCAGTATAC CTGCTCCTTC CAGGGTTTGT 
CACTACCCTG AGTTTCAGAG TCAGAATCAA CCTCAACCCA GTCAATCAAC CAATTCCCAC 
AGGATAAAAA TTAGGCCATG GATCGCCCCG GTGTCTGACA CTAATACAAA TATCAAGTCC 
ACGAGTAATA CTCATCTTCA GCCATCTCAC AAACACAGAC CCAAGACTCG ACCACCACAG 
GACCAAGGCC AAAGTCAGAC TAAATCAAAG GCTTCTGTCA TTTTAACCTC TAGTCAGAAT 
CAAAGCCACA CATCTAGACC CAAGCCTAAC TTTTATCCAA GCTCTAAACA GCTGGCTGAT 
GTGTCCCAAA CTAGAAACAC ATCACAGCTT GCGCAAATCC AGGGAAGCAG GTCCAGTCAC 
AAGCCCCGAC CTCCATTTCA ACCAAATTCT CAACATAGCC CCACAAGAGC AAAGCATTTA 
GTCCCTACTG CTCGTGAAAT CAATCCTCTT CATAATTCTC AATCTACCTC CAGAGCTGCT 
AGTAATCCTG GTTGTGGGCC AAACTCTCAG TCCATCCCTG GCCTCAAAAA ACACAGGTCC 
AAGTCTTGGG AGGCCACTGC CCCCACCACT GTGAATGTTA ATCACGCGAA AACAACACCG 
AAAAAACCTC AAACTAAGAA ACAGGAGGTG GAACGCAGAA GAGATCATCT GACCACAGGA 
GAGGGGAGAA AACACGAGAG AAAGGAAGAT AGACAAAAGG AAAAACAGCA AGGGAAACGG 
GAGCAAAAGG AAGACAGGAG GCTGGCTGAG GAGCAGCTAT TGAGACGGTC CTGGATCCAG 
AGTTCGGCTG AAGAAGAGGA GGGGGAAGAG GAGGAAGGAG TGACGGAACA GGACAGGAGG 
AGAGAGGAGA CGGCAAGAGA GGAAAGTCGG AGGAAGAGGG AGGCGCAACA ACAGCGACAT 
AAATGGGAGG CCAAACATAA AGTCCCTACC AACCACTCAC ATCAGCAAGG GGGGACAAAA 
AAGCGGGGAA GAAGTGAGGA GGAGAGACAG TTCCAGCCAG ACCCGTCGCC TCCTTCATCT 
TCACGGTCCC CAACTCCTCA GAGAACATCC TCCTCATCCT CTTCTTCGTC CTCATCGAGT 
TCAGATTCTG AATCTGAATT TCAAGCTCCG ATTACCAAAG TCCCAGCAGA CTCCACCTCT 
CAGAGGAAAC TCGCCAAGCC AGGGCAACAG AGCCCAGGAA GGCCTGACGC CAGCAGGCCT 
AAGGTGGTGC AGCAGAGACA ACCGAGCTCA GGTAACCCGA GTGAGGACCA GCAAAGTGCA 
GGGAAGCAAA AACTCTACAC CCTCGTCCCC TTTGGACGAG GGAACAAGGC TGCACCCCAC 
TCCCAGCGAG GGCTCCGAAA TCTGGTGGTG CACATAGACC TCTGTCTTCT CAAGAGGGTC 
CCAGACACTG CTACTCAATC CACTCCTAAA AAAACCTCCT CTTCCTCCTC ATCCTTATCA 
GCCAAGGACA AGCAAATTGA GACTATGAAA CACCTGCACG TCCCTGAGAC ACAGACTAAA 
GACAGCACAA GAAAGCGCAA GCTGGAGAAT GGTGTGTCAC ACAGAGAAAA CAAGAGGAGT 
TATGCAGACA AAACCTCAGG CCACACAGAG TCGTCATCTT TCACAGCTGA GACCGTAACG 
ACTGATACCG TGGACAGTGG GTGTGTGGAG GACGATTTGG ACAGTAAGAG GCCGCTGTCT 
CCGCTGTCTC CCTTGTCTCC CCGGCCCCAG AGCCCCGAGA TCCCCAAGTC TAACTCCAAA 
ACAAAAGCTG CAGAACAGCA TCACACCCAC AAGAACAAAG ACAAGAACAG AGATTCTGCG 
ATAAAGCCCA AGATGGAAGT GGAGTGTGTG AAAGTGTCGA AGCAGCCCCA GCCACCACCT 
GAGTCCTGGA GCACTGCAGC ACAAAGGGGG ACTGTGCCAA ACCACGAAAC TCCTCACCAT 
GCTGAGTACT ACTTACATGA AGCCAAAAGG ATGAAGCACC GTGCTGATGC CATGGTCGAC 
AAGCTAGGGA AGGCTGTGAA CTATGTTGAT GCTGCTCTCT CGTTCATGGA ATGTGGGAAG 
GCAATGGAGG AAGGGCCGCT CGAAGCAAAG TCTCCTTATA CCATGTATTC GGAAACAGTG 
GAGCTCATTA GGTACGCAAT GAAGCTGAAG AGCCACTCTG GCCCCGGGGC GAGACAGGAA 
GACAAACAGC TGGCGGTTCT GTGTTTCCGA TGCCTGGCCC TCCTTTACTG GCAGATGTTT 
CGCTTGAAGA AGGATCACGC GCTGAAATAC TCCAAAGTGC TGCTGGATTA CTTTAAGAGC 
TCTCCTAAAG TGCCTACTAC CCCACCCGGT TGGAGTGACT CTGGGAAGGG TACAGGAGGA 
CCCCCCTCTT CACTCTCGCC TAATACCAAA CACCTCGCAC GGGTTTCCCA CGAGGGCAGC 
AGCTCTGCCT CCCTCATAAG CATTCCCCAC CGTATCCACC AGATGGCAGC AACTCACCTG 
AACATTACCA ACAGTGTGCT GTACAGCTAT GAGTACTGGG AGGTGGCAGA CAACCTTGCA 
AAGGAGAATA AAGAGTTCTT CAACTACTTG AATACTTTGT CTGGGCCATT GACTCTTCAC 
AGTAGCATCG CTCACGCTGT CCAGTACACC AGGCAGGCCC TTCAGTGGAT ACGCATTAGT 
GCCAAACTCA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004551318.2
CDS	171..3845
Translation	MPTVLAGKGKLSIVCFLLRCAYSQERGSQHNSCAKDIFEDMTQSWRDFGDSLRRDPAQRILYNSKDTKQPITQQRQNRGDVPLRMARHPHVAPHKSMLADDLKLSSDDEETPTVIHESASWDRHSLSAQRAHTHGRRVRHSSSDSSGSDSTNESGSSSFHSRSPSRSPEVHPDPESPANIQPLCNNKEPDHPPAAQWQLDKWLKKSRKKCASTEQNPSQTIPGRLHSPHTHRAPSPARSFDSNQEYSPSQSPIPSPQFSYSNNNSPLPSPGYSYCPSPSPFTSTCQSPSPSPRHSPIPSPVLSICPSPCGTLRDSRSPSPIARDPPRSPSPSIPAPSRVCHYPEFQSQNQPQPSQSTNSHRIKIRPWIAPVSDTNTNIKSTSNTHLQPSHKHRPKTRPPQDQGQSQTKSKASVILTSSQNQSHTSRPKPNFYPSSKQLADVSQTRNTSQLAQIQGSRSSHKPRPPFQPNSQHSPTRAKHLVPTAREINPLHNSQSTSRAASNPGCGPNSQSIPGLKKHRSKSWEATAPTTVNVNHAKTTPKKPQTKKQEVERRRDHLTTGEGRKHERKEDRQKEKQQGKREQKEDRRLAEEQLLRRSWIQSSAEEEEGEEEEGVTEQDRRREETAREESRRKREAQQQRHKWEAKHKVPTNHSHQQGGTKKRGRSEEERQFQPDPSPPSSSRSPTPQRTSSSSSSSSSSSSDSESEFQAPITKVPADSTSQRKLAKPGQQSPGRPDASRPKVVQQRQPSSGNPSEDQQSAGKQKLYTLVPFGRGNKAAPHSQRGLRNLVVHIDLCLLKRVPDTATQSTPKKTSSSSSSLSAKDKQIETMKHLHVPETQTKDSTRKRKLENGVSHRENKRSYADKTSGHTESSSFTAETVTTDTVDSGCVEDDLDSKRPLSPLSPLSPRPQSPEIPKSNSKTKAAEQHHTHKNKDKNRDSAIKPKMEVECVKVSKQPQPPPESWSTAAQRGTVPNHETPHHAEYYLHEAKRMKHRADAMVDKLGKAVNYVDAALSFMECGKAMEEGPLEAKSPYTMYSETVELIRYAMKLKSHSGPGARQEDKQLAVLCFRCLALLYWQMFRLKKDHALKYSKVLLDYFKSSPKVPTTPPGWSDSGKGTGGPPSSLSPNTKHLARVSHEGSSSASLISIPHRIHQMAATHLNITNSVLYSYEYWEVADNLAKENKEFFNYLNTLSGPLTLHSSIAHAVQYTRQALQWIRISAKLN

Target ORF information:

RefSeq Version	XM_004551261.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra AF4/FMR2 family member 3 (aff3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004551261.4

ORF Insert Sequence:

ATGCCTACTG TCCTCGCTGG CAAAGGGAAA CTTTCCATTG TCTGCTTTCT GCTCCGATGC 
GCATATTCTC AGGAAAGAGG AAGCCAACAC AACAGCTGTG CCAAAGATAT ATTTGAGGAC 
ATGACCCAGT CCTGGAGGGA CTTTGGGGAC TCCTTGAGGC GTGACCCAGC ACAACGAATC 
CTGTACAACT CTAAGGATAC CAAACAGCCA ATTACACAAC AAAGGCAAAA CCGAGGGGAT 
GTTCCGCTGC GAATGGCCCG TCACCCTCAT GTGGCCCCCC ACAAATCCAT GCTCGCCGAT 
GACTTGAAGC TGAGCAGTGA TGATGAGGAA ACTCCCACGG TGATTCATGA GTCAGCTTCC 
TGGGACAGAC ACAGCCTGTC AGCACAGCGA GCGCACACAC ATGGCAGGCG GGTGAGACAT 
TCCAGCTCAG ACTCCTCAGG CTCAGACTCC ACCAATGAGT CAGGCAGCAG TAGCTTTCAT 
TCCCGGAGCC CAAGCCGGAG TCCAGAAGTT CACCCTGATC CCGAGTCGCC TGCCAACATC 
CAGCCACTGT GCAACAACAA GGAGCCTGAT CACCCCCCTG CTGCCCAGTG GCAGTTAGAT 
AAATGGCTGA AGAAGTCGCG GAAAAAATGT GCCAGCACTG AGCAAAATCC CTCCCAGACT 
ATTCCAGGAC GCCTGCACTC TCCCCACACC CACCGAGCCC CATCCCCAGC AAGGTCATTT 
GACAGCAATC AGGAATACAG CCCTAGCCAA AGCCCTATTC CCAGCCCACA GTTCAGTTAC 
AGCAACAACA ACAGCCCCCT TCCAAGCCCT GGTTACAGCT ACTGTCCAAG TCCGAGCCCA 
TTTACCAGCA CTTGTCAAAG TCCCAGCCCT AGTCCAAGGC ACAGCCCAAT TCCAAGTCCA 
GTTCTAAGTA TTTGCCCCAG TCCATGTGGG ACACTAAGAG ACAGCCGTAG TCCTAGCCCT 
ATAGCTAGAG ATCCTCCAAG AAGCCCGAGT CCCAGTATAC CTGCTCCTTC CAGGGTTTGT 
CACTACCCTG AGTTTCAGAG TCAGAATCAA CCTCAACCCA GTCAATCAAC CAATTCCCAC 
AGGATAAAAA TTAGGCCATG GATCGCCCCG GTGTCTGACA CTAATACAAA TATCAAGTCC 
ACGAGTAATA CTCATCTTCA GCCATCTCAC AAACACAGAC CCAAGACTCG ACCACCACAG 
GACCAAGGCC AAAGTCAGAC TAAATCAAAG GCTTCTGTCA TTTTAACCTC TAGTCAGAAT 
CAAAGCCACA CATCTAGACC CAAGCCTAAC TTTTATCCAA GCTCTAAACA GCTGGCTGAT 
GTGTCCCAAA CTAGAAACAC ATCACAGCTT GCGCAAATCC AGGGAAGCAG GTCCAGTCAC 
AAGCCCCGAC CTCCATTTCA ACCAAATTCT CAACATAGCC CCACAAGAGC AAAGCATTTA 
GTCCCTACTG CTCGTGAAAT CAATCCTCTT CATAATTCTC AATCTACCTC CAGAGCTGCT 
AGTAATCCTG GTTGTGGGCC AAACTCTCAG TCCATCCCTG GCCTCAAAAA ACACAGGTCC 
AAGTCTTGGG AGGCCACTGC CCCCACCACT GTGAATGTTA ATCACGCGAA AACAACACCG 
AAAAAACCTC AAACTAAGAA ACAGGAGGTG GAACGCAGAA GAGATCATCT GACCACAGGA 
GAGGGGAGAA AACACGAGAG AAAGGAAGAT AGACAAAAGG AAAAACAGCA AGGGAAACGG 
GAGCAAAAGG AAGACAGGAG GCTGGCTGAG GAGCAGCTAT TGAGACGGTC CTGGATCCAG 
AGTTCGGCTG AAGAAGAGGA GGGGGAAGAG GAGGAAGGAG TGACGGAACA GGACAGGAGG 
AGAGAGGAGA CGGCAAGAGA GGAAAGTCGG AGGAAGAGGG AGGCGCAACA ACAGCGACAT 
AAATGGGAGG CCAAACATAA AGTCCCTACC AACCACTCAC ATCAGCAAGG GGGGACAAAA 
AAGCGGGGAA GAAGTGAGGA GGAGAGACAG TTCCAGCCAG ACCCGTCGCC TCCTTCATCT 
TCACGGTCCC CAACTCCTCA GAGAACATCC TCCTCATCCT CTTCTTCGTC CTCATCGAGT 
TCAGATTCTG AATCTGAATT TCAAGCTCCG ATTACCAAAG TCCCAGCAGA CTCCACCTCT 
CAGAGGAAAC TCGCCAAGCC AGGGCAACAG AGCCCAGGAA GGCCTGACGC CAGCAGGCCT 
AAGGTGGTGC AGCAGAGACA ACCGAGCTCA GGTAACCCGA GTGAGGACCA GCAAAGTGCA 
GGGAAGCAAA AACTCTACAC CCTCGTCCCC TTTGGACGAG GGAACAAGGC TGCACCCCAC 
TCCCAGCGAG GGCTCCGAAA TCTGGTGGTG CACATAGACC TCTGTCTTCT CAAGAGGGTC 
CCAGACACTG CTACTCAATC CACTCCTAAA AAAACCTCCT CTTCCTCCTC ATCCTTATCA 
GCCAAGGACA AGCAAATTGA GACTATGAAA CACCTGCACG TCCCTGAGAC ACAGACTAAA 
GACAGCACAA GAAAGCGCAA GCTGGAGAAT GGTGTGTCAC ACAGAGAAAA CAAGAGGAGT 
TATGCAGACA AAACCTCAGG CCACACAGAG TCGTCATCTT TCACAGCTGA GACCGTAACG 
ACTGATACCG TGGACAGTGG GTGTGTGGAG GACGATTTGG ACAGTAAGAG GCCGCTGTCT 
CCGCTGTCTC CCTTGTCTCC CCGGCCCCAG AGCCCCGAGA TCCCCAAGTC TAACTCCAAA 
ACAAAAGCTG CAGAACAGCA TCACACCCAC AAGAACAAAG ACAAGAACAG AGATTCTGCG 
ATAAAGCCCA AGATGGAAGT GGAGTGTGTG AAAGTGTCGA AGCAGCCCCA GCCACCACCT 
GAGTCCTGGA GCACTGCAGC ACAAAGGGGG ACTGTGCCAA ACCACGAAAC TCCTCACCAT 
GCTGAGTACT ACTTACATGA AGCCAAAAGG ATGAAGCACC GTGCTGATGC CATGGTCGAC 
AAGCTAGGGA AGGCTGTGAA CTATGTTGAT GCTGCTCTCT CGTTCATGGA ATGTGGGAAG 
GCAATGGAGG AAGGGCCGCT CGAAGCAAAG TCTCCTTATA CCATGTATTC GGAAACAGTG 
GAGCTCATTA GGTACGCAAT GAAGCTGAAG AGCCACTCTG GCCCCGGGGC GAGACAGGAA 
GACAAACAGC TGGCGGTTCT GTGTTTCCGA TGCCTGGCCC TCCTTTACTG GCAGATGTTT 
CGCTTGAAGA AGGATCACGC GCTGAAATAC TCCAAAGTGC TGCTGGATTA CTTTAAGAGC 
TCTCCTAAAG TGCCTACTAC CCCACCCGGT TGGAGTGACT CTGGGAAGGG TACAGGAGGA 
CCCCCCTCTT CACTCTCGCC TAATACCAAA CACCTCGCAC GGGTTTCCCA CGAGGGCAGC 
AGCTCTGCCT CCCTCATAAG CATTCCCCAC CGTATCCACC AGATGGCAGC AACTCACCTG 
AACATTACCA ACAGTGTGCT GTACAGCTAT GAGTACTGGG AGGTGGCAGA CAACCTTGCA 
AAGGAGAATA AAGAGTTCTT CAACTACTTG AATACTTTGT CTGGGCCATT GACTCTTCAC 
AGTAGCATCG CTCACGCTGT CCAGTACACC AGGCAGGCCC TTCAGTGGAT ACGCATTAGT 
GCCAAACTCA ACTAA