BUB1B cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1B
Entrez Gene ID	108508851
Full Name	BUB1 mitotic checkpoint serine/threonine kinase B
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017837970.1	XP_017693459.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Pathways from BioSystems
Gene Ontology

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe74407	XM_017837970.1 Latest version!	Lepidothrix coronata BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1B ( XM_017837970.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017693459.1 Lepidothrix coronata BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

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CloneID	OLe74407
Clone ID Related Accession (Same CDS sequence)	XM_017837970.1
Accession Version	XM_017837970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGGGGAGAA GGTGGACGTT TGAATTTCGG GGCGGGCTGA GGCGGCGCTG CCGTTACTGC 
GCGAGGCTGA GGGGCGCGGG CCGTGAGGGA CGCGCGGAGC CGAAAGGTAC GCGGGGCTCA 
GGGGACAGAG GGGCCCGGGA CTGTGAGGGT GGGGTTACTG ACCTGGGCTT GTTTCAGGGC 
AATTGCTGTC ACGAGCCCTT GGATCTGTAC AGTTACCTGT GCAGCCAGGA GATTGGCACC 
ACGCTGGCAC TGCTCTACAT CACCTGGGCA GAGGCACTGG AGGCCAGAGG GAACTTCAGG 
AAAGCAGATC TGATATTCCA GGAAGGGCTT CAGCGCAGGG CTGAGCCTTT GGACAAACTG 
CAGTCCCATC ACAGGCAGTT TCAGGCCCGC GTTTCTCGGC AGGCACTGCT GGCACTTGAG 
GAAAGTCCAG ATGGAAAAGA TACAGGTCTG CTGGAAATTG CAGAGCCTCA GAGAAGCTCA 
TTGGCAGATC TCAAAGGCAG GGGGAAGAAA AAAGTGAGAG CGCCCATTAG TCGTGTGGGA 
GATGCAGTTA AAGTCACGAA CCCAAACAGA AGCCTCATGC CTCAGACTTC TCTGCAGCTT 
TCAAATACTC CGGGTTTTGC TGTGTTTGAT GAAAATTCAG CCTCTGTAGA CGAGATTCCC 
ACGCTTACAG CACAGTCATG GACAGCACCT CCAGCTCCCA GGGCCAAGGA GAATGAACTC 
AGTGCAGGAC CTTGGAATTC TGGCAAGCGT CCGCGCAGCC GCAGCGCCTC CAACCCTGGC 
GTGGCAGTGC CCGGCCCCCT GCCCAGCTTC ACCCCCTACG TGGACGAGTC AGCTCAGCCT 
CAAATGATGA CTCCGTGCAA AATCGAGCCC AGCATAAACC ACGTGCTGAG CGCTCGCAAA 
CCTGAGAAGG AGGACCCGCT GCAGCGAGTG CAGCACCATC AGCAGGACAC TCAGGAGCAG 
AGGGAGATGG TGATGTACTG CAAGGACAAG GTTTATGCTG GAGTCGATGA ATTCTCTTTG 
GAAGAGATTC GAGCTGAAAT CTACAGGAAG AAAGCGAAAA AGAAAACTGA AGAGGAAATG 
CAAGCCATAG CACAGAAGAA GGAAGAAATA CAAAGGCAAA TTGAGGAGCT GGAGAAGAAA 
CTGAAAGAAG ATGACAAGCA GCAACAACCA CGTGAACAGG CAGCAGAGAT AAGGGAAGCT 
TTGGCACCTT TGGGACCGCA AGGATTTACT TCTTCCAGTG GAACAGAAGT TGGGGAGAAG 
CAGCTTCAGG GTCAAAGAGT AAATCAGAGT GCGGACAGAA AGCTTCCAGT ATCCGGGGTA 
CTTTTACTGA CAGAGGTAAT CCAATGGCAG AGACTCATTA CTGAGTTCCA GCTCACCTTG 
CTGTTCCTTG CCTTCATAGG GGATGATGTG GCTCAGTTCC TGGGGAGTGC TGAGCAGCAT 
AAAAAGCTCT TGGATCCTGT GCCAGAATCA CTGGCTGCTT CTCCAGATTT TTACTTGGAG 
CCTGGAGCTC TGCCTGGCAT GGAGGTTGAG AAGGACATTG AGCTGGGGCA TGAGACTTAC 
TGCATCAAAT GGGAATACTG GAACAATGAA GAATACAAAA TGTTTTTTGC CGTTCCAGCC 
AACCTTTTCC AGCTGGATGC AAAGGGATTT GCAATAAAGG TGTATTCTCA GCCTGTGCCT 
TGGGATTTTT ATATCACCCT TGAGTTACAG AGGCGTTTGA AGGCTGACTT TGACCAGAGC 
TTCAGCGAGA GCTGCAGCTG CCACCTGTAC CGGGATGGCT GTGTCGTGGT GCACAGGGAC 
ATCAACCGCT TCACGCTCGG GGATGTTATC CGTGGCCGCA AGTCCATCAC TGAGGAAGTC 
GTCTTCCTGG TTGTTTATAA TCTTCTGGGT GTGGTAGAGA AGCTGCACAA GGCTGAGATT 
GTCCATGGAG ATCTGAGACC AGAGGTGTTC TTTCTGGGAG ACAGGATCTG TGATGCCTTT 
GCTAACGAGG ACATGGCGAG TGCTCTGAAG GTGGTGGATT TCTCTCACAG TCTGGATTTG 
AGGTTGCAGT CTCGAGTGAG CTCGTGCAAC AGCTTTCCCA TATCTCAGAC CCCTCATGGG 
CAGCAGCTTC TGGCAGCAAG TTCTCTTCCT TATCAGGTAG ACTTGGTTGG CATTGCAGAT 
ATAGTCCATT TGATGCTGTT TGGGGATCAT ATCCAGGTCT ATCAGGAGAA TTCCATCTGG 
AAAATCAGCC AAAACTTACC AAAGACTGTT GACAGTGATT TCTGGAGTAA ACTTTTTGGG 
AGAGTTCTGA ATGCAGGTGG TAAGTCCACA GCGCCTCTGC TGCGGGAGTT GAGAGAGGAA 
ATTGGTGACA TGTTTGACAG CTGTTTCCAA GAACGACTTT GTGAGTCCTT AGCTGCCCTG 
GGAGTAACCT TCCTCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017693459.1
CDS	1..2421
Translation	MGRRWTFEFRGGLRRRCRYCARLRGAGREGRAEPKGTRGSGDRGARDCEGGVTDLGLFQGNCCHEPLDLYSYLCSQEIGTTLALLYITWAEALEARGNFRKADLIFQEGLQRRAEPLDKLQSHHRQFQARVSRQALLALEESPDGKDTGLLEIAEPQRSSLADLKGRGKKKVRAPISRVGDAVKVTNPNRSLMPQTSLQLSNTPGFAVFDENSASVDEIPTLTAQSWTAPPAPRAKENELSAGPWNSGKRPRSRSASNPGVAVPGPLPSFTPYVDESAQPQMMTPCKIEPSINHVLSARKPEKEDPLQRVQHHQQDTQEQREMVMYCKDKVYAGVDEFSLEEIRAEIYRKKAKKKTEEEMQAIAQKKEEIQRQIEELEKKLKEDDKQQQPREQAAEIREALAPLGPQGFTSSSGTEVGEKQLQGQRVNQSADRKLPVSGVLLLTEVIQWQRLITEFQLTLLFLAFIGDDVAQFLGSAEQHKKLLDPVPESLAASPDFYLEPGALPGMEVEKDIELGHETYCIKWEYWNNEEYKMFFAVPANLFQLDAKGFAIKVYSQPVPWDFYITLELQRRLKADFDQSFSESCSCHLYRDGCVVVHRDINRFTLGDVIRGRKSITEEVVFLVVYNLLGVVEKLHKAEIVHGDLRPEVFFLGDRICDAFANEDMASALKVVDFSHSLDLRLQSRVSSCNSFPISQTPHGQQLLAASSLPYQVDLVGIADIVHLMLFGDHIQVYQENSIWKISQNLPKTVDSDFWSKLFGRVLNAGGKSTAPLLRELREEIGDMFDSCFQERLCESLAALGVTFLL

Target ORF information:

RefSeq Version	XM_017837970.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017837970.1

ORF Insert Sequence:

ATGGGGAGAA GGTGGACGTT TGAATTTCGG GGCGGGCTGA GGCGGCGCTG CCGTTACTGC 
GCGAGGCTGA GGGGCGCGGG CCGTGAGGGA CGCGCGGAGC CGAAAGGTAC GCGGGGCTCA 
GGGGACAGAG GGGCCCGGGA CTGTGAGGGT GGGGTTACTG ACCTGGGCTT GTTTCAGGGC 
AATTGCTGTC ACGAGCCCTT GGATCTGTAC AGTTACCTGT GCAGCCAGGA GATTGGCACC 
ACGCTGGCAC TGCTCTACAT CACCTGGGCA GAGGCACTGG AGGCCAGAGG GAACTTCAGG 
AAAGCAGATC TGATATTCCA GGAAGGGCTT CAGCGCAGGG CTGAGCCTTT GGACAAACTG 
CAGTCCCATC ACAGGCAGTT TCAGGCCCGC GTTTCTCGGC AGGCACTGCT GGCACTTGAG 
GAAAGTCCAG ATGGAAAAGA TACAGGTCTG CTGGAAATTG CAGAGCCTCA GAGAAGCTCA 
TTGGCAGATC TCAAAGGCAG GGGGAAGAAA AAAGTGAGAG CGCCCATTAG TCGTGTGGGA 
GATGCAGTTA AAGTCACGAA CCCAAACAGA AGCCTCATGC CTCAGACTTC TCTGCAGCTT 
TCAAATACTC CGGGTTTTGC TGTGTTTGAT GAAAATTCAG CCTCTGTAGA CGAGATTCCC 
ACGCTTACAG CACAGTCATG GACAGCACCT CCAGCTCCCA GGGCCAAGGA GAATGAACTC 
AGTGCAGGAC CTTGGAATTC TGGCAAGCGT CCGCGCAGCC GCAGCGCCTC CAACCCTGGC 
GTGGCAGTGC CCGGCCCCCT GCCCAGCTTC ACCCCCTACG TGGACGAGTC AGCTCAGCCT 
CAAATGATGA CTCCGTGCAA AATCGAGCCC AGCATAAACC ACGTGCTGAG CGCTCGCAAA 
CCTGAGAAGG AGGACCCGCT GCAGCGAGTG CAGCACCATC AGCAGGACAC TCAGGAGCAG 
AGGGAGATGG TGATGTACTG CAAGGACAAG GTTTATGCTG GAGTCGATGA ATTCTCTTTG 
GAAGAGATTC GAGCTGAAAT CTACAGGAAG AAAGCGAAAA AGAAAACTGA AGAGGAAATG 
CAAGCCATAG CACAGAAGAA GGAAGAAATA CAAAGGCAAA TTGAGGAGCT GGAGAAGAAA 
CTGAAAGAAG ATGACAAGCA GCAACAACCA CGTGAACAGG CAGCAGAGAT AAGGGAAGCT 
TTGGCACCTT TGGGACCGCA AGGATTTACT TCTTCCAGTG GAACAGAAGT TGGGGAGAAG 
CAGCTTCAGG GTCAAAGAGT AAATCAGAGT GCGGACAGAA AGCTTCCAGT ATCCGGGGTA 
CTTTTACTGA CAGAGGTAAT CCAATGGCAG AGACTCATTA CTGAGTTCCA GCTCACCTTG 
CTGTTCCTTG CCTTCATAGG GGATGATGTG GCTCAGTTCC TGGGGAGTGC TGAGCAGCAT 
AAAAAGCTCT TGGATCCTGT GCCAGAATCA CTGGCTGCTT CTCCAGATTT TTACTTGGAG 
CCTGGAGCTC TGCCTGGCAT GGAGGTTGAG AAGGACATTG AGCTGGGGCA TGAGACTTAC 
TGCATCAAAT GGGAATACTG GAACAATGAA GAATACAAAA TGTTTTTTGC CGTTCCAGCC 
AACCTTTTCC AGCTGGATGC AAAGGGATTT GCAATAAAGG TGTATTCTCA GCCTGTGCCT 
TGGGATTTTT ATATCACCCT TGAGTTACAG AGGCGTTTGA AGGCTGACTT TGACCAGAGC 
TTCAGCGAGA GCTGCAGCTG CCACCTGTAC CGGGATGGCT GTGTCGTGGT GCACAGGGAC 
ATCAACCGCT TCACGCTCGG GGATGTTATC CGTGGCCGCA AGTCCATCAC TGAGGAAGTC 
GTCTTCCTGG TTGTTTATAA TCTTCTGGGT GTGGTAGAGA AGCTGCACAA GGCTGAGATT 
GTCCATGGAG ATCTGAGACC AGAGGTGTTC TTTCTGGGAG ACAGGATCTG TGATGCCTTT 
GCTAACGAGG ACATGGCGAG TGCTCTGAAG GTGGTGGATT TCTCTCACAG TCTGGATTTG 
AGGTTGCAGT CTCGAGTGAG CTCGTGCAAC AGCTTTCCCA TATCTCAGAC CCCTCATGGG 
CAGCAGCTTC TGGCAGCAAG TTCTCTTCCT TATCAGGTAG ACTTGGTTGG CATTGCAGAT 
ATAGTCCATT TGATGCTGTT TGGGGATCAT ATCCAGGTCT ATCAGGAGAA TTCCATCTGG 
AAAATCAGCC AAAACTTACC AAAGACTGTT GACAGTGATT TCTGGAGTAA ACTTTTTGGG 
AGAGTTCTGA ATGCAGGTGG TAAGTCCACA GCGCCTCTGC TGCGGGAGTT GAGAGAGGAA 
ATTGGTGACA TGTTTGACAG CTGTTTCCAA GAACGACTTT GTGAGTCCTT AGCTGCCCTG 
GGAGTAACCT TCCTCCTCTA G