HECW1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	108501343
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017823150.1	XP_017678639.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_017823109.1	XP_017678598.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_017823179.1	XP_017678668.1	E3 ubiquitin-protein ligase HECW1 isoform X8
XM_017823140.1	XP_017678629.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_017823131.1	XP_017678620.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_017823121.1	XP_017678610.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_017823159.1	XP_017678648.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_017823169.1	XP_017678658.1	E3 ubiquitin-protein ligase HECW1 isoform X7

Pathways from BioSystems
Gene Ontology

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLe49378	XM_017823109.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLe49379	XM_017823121.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLe49380	XM_017823131.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLe49381	XM_017823140.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLe49382	XM_017823150.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLe49383	XM_017823159.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLe49384	XM_017823169.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLe49385	XM_017823179.1 Latest version!	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( XM_017823109.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678598.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLe49378
Clone ID Related Accession (Same CDS sequence)	XM_017823109.1
Accession Version	XM_017823109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGACTC AATCCAGGGA 
CAAGGAAATC GGAGACTGAT AAGTTTTTCA TTGTCAGATT TCCAGGCCTT TGGTCTGAAG 
AAAGGAATGT TCTTTAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGAAAACAT 
AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GATCTAAGAT CATGTGTAAT 
ACCGTCAACC CGATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCCCTGCC CACGGACGTC 
CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA ACGTTTTCTG 
GGAAAACTTT CTATGCCGGT TCAACGCCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT 
GTAAGCTACA CTCTGGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTT 
CGATTTGAAA TAACTTCTTC CATCCATCCA GATGATGAAG AGGTCTCCAT TAGTGCAGAT 
CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAATC CCACAGAGGA AAGCCTCGGT 
GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAGTGCAG CTCCAGAACA GCTAAATGGA 
TACAGTGTGA ACAGTGTCGA TAGCCCAGGG GCTGTCAAAC CACCTGGGGA AGTCAACCAG 
AACAGCTTAA ATGAAGAGCT AGCAGGAGAT GGGGAGGTTG ATGCGGTGCC AGTTCCTGAA 
CCCTTGGAAT CCATCCCAGG AGAAATGCTT TCTTCTTTGA GTGCTACGGA CCTCACAGAG 
CAGTCGACTC TGATGGCTTG CATGTCTCCT CCTGAGACTG AAGTTAGGGA AAATAACAAA 
GTCAATTCTG GTATTCAGGG AGATGGTGGA GTGACCAGCA TAGAAACATT AGATATTGAT 
GAGGTGAGTG CAGATGTGCG TGCTGGCAAG GACTTAGAGA AAAGTCAGGA AGAAGATGAA 
GGGGCTTCAG CCCAGGAGGA GGAAGACAAC AAGCTACAAC TGAGGACCAC AGCAAAGAGG 
AAAAACAGGC CGTGCTCCCT GCCTGTGTCT GAACTTGAGA CGGTCATAGC TTCAGCCTGC 
GGTGAGCCAG AGACCCCTCG CACACACTAC ATACGGATCC ACAACCTACT CCACAGCCTG 
CCCTCGGCAC AGATGAGCAG CGATGGGGAA GAAGAACAAG ATGGTGGCAA TGGCGGGGAG 
AACGATGAGG AATCTACAGT CAAGGAGGTG TTGGATAAGG AAGTGGTCAG TGAGAGTGAG 
ACCGTGGCAG CAGAGCCTCC TCTGGAAAAT GAGGCTGAAG AGAACTTCAG CCAGAGAACA 
GTGCCTCCTG AGACCTTGGA TGAGCAACTC TCTGACTTAA ATGATGGGCT GTTGGATGGG 
GAGCCCCTCA GGACAGGAAG CGAGAGCGAG TCAAGCTCCA GGCCCAATGG CGACAACGAG 
TGCGAAGCGT GCGACACCTC TTGCTACAGC CCCTCTTGCT ACAGCACTTC CTGCTACAGC 
ACTTCCTGCT ACAGCACTTC TTGCTACAGC ACCTCGTGTT ATGACAGTAA CAATCGCTTC 
TCCAGCCATA CTCGCTTCTC CTCCGTCGAC AGCGCAAAGA TTTCTGAGAG CACAGTCTTT 
TCCTCACAGG ATGATGATGA GGAGGAGAAC AGTGCTTTTG AGTCAGTGCC AGATTCAGTG 
CAGAGCCCTG AGCTGGACAG GGAGCAAGTG GATGGCTCCT CCCAGTGGCC AGATGAACTA 
GTAGCTCATG CTGGGAATCC ACAAAGAGCC ACAGAGGGTC TAGACACCCC AATAGCAGGT 
CCGAGCAGCA GAAGAGAAGG TGATTGTCCT TTACTCCATA ATTCTCAGCC AGTCAGCCAG 
CTTCCTTCTC TGAGGCCTGA CCATCATCAC TACCCAACTA TCGATGAGCC TCTTCCACCA 
ACTACAACAT CCTTGGGAAA CTTTACCTGG TGGAATTGGG AAGCTCGGAT AGACAGCCAC 
GGGAGGGTAT TCTACGTTGA TCATGTCAAC CGAACCACCA CGTGGCAGCG CCCCACAGCC 
GCGGCAACCC CGGATGGGAT CCACAGGTCT GGCTCCATCC AGCAAATGGA GCAGCTGAAC 
CGGCGATACC AAAACATCCA GCGAACAATC GCAACAGAGC GGACTGACGA TGATGCTGTG 
GTAAACAACA GGGTGGAGAG AGTTCCCACT GGAGGAGGAA GTGACTCTGA GGCAGAGCCT 
TCTCAGCCAA GCTCAGAGAT TAGGAGGGAA GGTTCCCTTT CTCCTGTGAA TTCTCAGAAA 
ATCACCTTGT TGCTGCAGTC TCCAGCTGTG AAGTTCATTA CCAATCCTGA GTTCTTCACT 
GTGCTGCATG CAAACTATAG TGCCTATCGG GTCTTCACTA ACAGTACCTG CTTGAAGCAT 
ATGATTCTGA AGATTCGTAG AGATGCTCGT AATTTTGAGC GGTATCAGCA CAACAGAGAC 
CTGGTAAATT TTATCAACAT GTTTGCTGAT ACCCGACTGG AGCTTCCTCG TGGCTGGGAG 
ATAAAAACTG ACCAGCAGGG CAAGTCTTTC TTCGTTGACC ACAATAGCCG TGCTACCACC 
TTCATAGATC CCAGGATTCC GCTGCAGAAT GGTCGTCTCC CTAATCACCT GACTCACAGG 
CAACATCTTC AGCGACTTCG TAGCTACAGC GCTGGAGAGG CCTCTGAAGT CTCTCGAAAC 
AGAGGAGTCA CCCTGTTGGC CAGACCAGGC AATAGTCTTG TAACAGCTAT TCGAAACCAG 
CACCATCATG AGGCATTACC TCTGGCTTAC AATGACAAGA TTGTTGCATT TTTACGCCAA 
CCCAACATTT TTGAGATGCT GCAGGAGCGT CAGCCCAGCT TGGCCAGAAA CCATGCACTC 
AGGGAGAAGA TCCATTACAT TCGGACAGAG GGTACACATG GGCTTGAAAA ATTGTCCTGT 
GATGCAGATT TAGTAATTCT GCTGAGCCTT TTTGAAGAGG ATATTATGTC CTACATACCA 
CTGCAGGCTG CCTTCCATCC TGGTTACAGT TTCTCTCCTC GCTGCTCACC CTGTTCATCA 
CCCCAAAATT CCCCAGGGCT GCAGAGAGCA AGTGCAAGAG CACCTTCTCC ATACAGGAGG 
GACTTTGAAG CCAAGCTGCG CAATTTCTAT CGAAAACTTG AAGCCAAAGG GTATGGGCAA 
GGTCCAGGTA AAATTAAGTT AATTATACGG CGTGACCACT TGCTGGAAGG CACCTTCAAC 
CAGGTAATGG CATATTCACG CAAGGAGCTC CAGCGGAACA AACTCTACGT CACGTTTGTT 
GGCGAGGAAG GGTTGGACTA CAGTGGCCCC TCCCGAGAAT TCTTCTTCCT TCTGTCTCAG 
GAGCTCTTCA ATCCTTATTA TGGGCTGTTT GAGTATTCAG CCAATGACAC TTACACTGTA 
CAGATCAGCC CCATGTCTGC CTTTGTTGAA AATCACCTGG AATGGTTCAG GTTCAGCGGT 
CGTATTCTTG GCCTTGCTCT CATTCATCAG TACCTTCTTG ATGCTTTCTT CACAAGGCCG 
TTCTACAAAG CACTACTAAG GCTACCATGT GATTTAAGTG ACTTAGAGTA CCTGGATGAA 
GAGTTCCATC AGAGCTTGCA GTGGATGAAG GATAACAACA TCACAGATAT CTTGGACTTA 
ACCTTCACAG TGAACGAGGA GGTATTTGGA CAGGTGACAG AGAGAGAGTT GAAATCTGGT 
GGGGCAAATA CAGCAGTGAC AGAAAAGAAC AAAAAAGAGT ACATCGAGAG GATGGTTAAG 
TGGCGAGTGG AACGAGGAGT GGTTCAGCAA ACAGAGGCCC TGGTCCGTGG CTTCTATGAA 
GTTGTAGACT CCAGGCTCGT CTCTGTTTTT GATGCCAGAG AACTGGAACT GGTTATAGCT 
GGCACTGCAG AAATAGATCT CAATGACTGG CGGAACAACA CAGAATATCG TGGAGGTTAC 
CATGATGGAC ACATAGTAAT ACGGTGGTTC TGGGCAGCAG TGGAGAGGTT TAACAACGAG 
CAGAGGCTCC GGCTGTTACA GTTCGTCACA GGAACATCCA GCGTGCCATA CGAGGGCTTT 
GCTGCTCTCC GAGGAAGCAA CGGGCTCCGG CGCTTCTGTA TAGAGAAATG GGGGAAAATA 
ACCTCCCTCC CAAGGGCACA TACATGTTTC AACCGTTTGG ATCTTCCACC TTACCCCTCA 
TATTCTATGC TGTATGAAAA GCTGCTGACA GCTGTTGAAG AAACTAGCAC CTTTGGACTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678598.1
CDS	165..5030
Translation	MSCAQAGIVQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPQIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPTTTSLGNFTWWNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823109.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823109.1

ORF Insert Sequence:

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGACTC AATCCAGGGA 
CAAGGAAATC GGAGACTGAT AAGTTTTTCA TTGTCAGATT TCCAGGCCTT TGGTCTGAAG 
AAAGGAATGT TCTTTAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGAAAACAT 
AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GATCTAAGAT CATGTGTAAT 
ACCGTCAACC CGATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCCCTGCC CACGGACGTC 
CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA ACGTTTTCTG 
GGAAAACTTT CTATGCCGGT TCAACGCCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT 
GTAAGCTACA CTCTGGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTT 
CGATTTGAAA TAACTTCTTC CATCCATCCA GATGATGAAG AGGTCTCCAT TAGTGCAGAT 
CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAATC CCACAGAGGA AAGCCTCGGT 
GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAGTGCAG CTCCAGAACA GCTAAATGGA 
TACAGTGTGA ACAGTGTCGA TAGCCCAGGG GCTGTCAAAC CACCTGGGGA AGTCAACCAG 
AACAGCTTAA ATGAAGAGCT AGCAGGAGAT GGGGAGGTTG ATGCGGTGCC AGTTCCTGAA 
CCCTTGGAAT CCATCCCAGG AGAAATGCTT TCTTCTTTGA GTGCTACGGA CCTCACAGAG 
CAGTCGACTC TGATGGCTTG CATGTCTCCT CCTGAGACTG AAGTTAGGGA AAATAACAAA 
GTCAATTCTG GTATTCAGGG AGATGGTGGA GTGACCAGCA TAGAAACATT AGATATTGAT 
GAGGTGAGTG CAGATGTGCG TGCTGGCAAG GACTTAGAGA AAAGTCAGGA AGAAGATGAA 
GGGGCTTCAG CCCAGGAGGA GGAAGACAAC AAGCTACAAC TGAGGACCAC AGCAAAGAGG 
AAAAACAGGC CGTGCTCCCT GCCTGTGTCT GAACTTGAGA CGGTCATAGC TTCAGCCTGC 
GGTGAGCCAG AGACCCCTCG CACACACTAC ATACGGATCC ACAACCTACT CCACAGCCTG 
CCCTCGGCAC AGATGAGCAG CGATGGGGAA GAAGAACAAG ATGGTGGCAA TGGCGGGGAG 
AACGATGAGG AATCTACAGT CAAGGAGGTG TTGGATAAGG AAGTGGTCAG TGAGAGTGAG 
ACCGTGGCAG CAGAGCCTCC TCTGGAAAAT GAGGCTGAAG AGAACTTCAG CCAGAGAACA 
GTGCCTCCTG AGACCTTGGA TGAGCAACTC TCTGACTTAA ATGATGGGCT GTTGGATGGG 
GAGCCCCTCA GGACAGGAAG CGAGAGCGAG TCAAGCTCCA GGCCCAATGG CGACAACGAG 
TGCGAAGCGT GCGACACCTC TTGCTACAGC CCCTCTTGCT ACAGCACTTC CTGCTACAGC 
ACTTCCTGCT ACAGCACTTC TTGCTACAGC ACCTCGTGTT ATGACAGTAA CAATCGCTTC 
TCCAGCCATA CTCGCTTCTC CTCCGTCGAC AGCGCAAAGA TTTCTGAGAG CACAGTCTTT 
TCCTCACAGG ATGATGATGA GGAGGAGAAC AGTGCTTTTG AGTCAGTGCC AGATTCAGTG 
CAGAGCCCTG AGCTGGACAG GGAGCAAGTG GATGGCTCCT CCCAGTGGCC AGATGAACTA 
GTAGCTCATG CTGGGAATCC ACAAAGAGCC ACAGAGGGTC TAGACACCCC AATAGCAGGT 
CCGAGCAGCA GAAGAGAAGG TGATTGTCCT TTACTCCATA ATTCTCAGCC AGTCAGCCAG 
CTTCCTTCTC TGAGGCCTGA CCATCATCAC TACCCAACTA TCGATGAGCC TCTTCCACCA 
ACTACAACAT CCTTGGGAAA CTTTACCTGG TGGAATTGGG AAGCTCGGAT AGACAGCCAC 
GGGAGGGTAT TCTACGTTGA TCATGTCAAC CGAACCACCA CGTGGCAGCG CCCCACAGCC 
GCGGCAACCC CGGATGGGAT CCACAGGTCT GGCTCCATCC AGCAAATGGA GCAGCTGAAC 
CGGCGATACC AAAACATCCA GCGAACAATC GCAACAGAGC GGACTGACGA TGATGCTGTG 
GTAAACAACA GGGTGGAGAG AGTTCCCACT GGAGGAGGAA GTGACTCTGA GGCAGAGCCT 
TCTCAGCCAA GCTCAGAGAT TAGGAGGGAA GGTTCCCTTT CTCCTGTGAA TTCTCAGAAA 
ATCACCTTGT TGCTGCAGTC TCCAGCTGTG AAGTTCATTA CCAATCCTGA GTTCTTCACT 
GTGCTGCATG CAAACTATAG TGCCTATCGG GTCTTCACTA ACAGTACCTG CTTGAAGCAT 
ATGATTCTGA AGATTCGTAG AGATGCTCGT AATTTTGAGC GGTATCAGCA CAACAGAGAC 
CTGGTAAATT TTATCAACAT GTTTGCTGAT ACCCGACTGG AGCTTCCTCG TGGCTGGGAG 
ATAAAAACTG ACCAGCAGGG CAAGTCTTTC TTCGTTGACC ACAATAGCCG TGCTACCACC 
TTCATAGATC CCAGGATTCC GCTGCAGAAT GGTCGTCTCC CTAATCACCT GACTCACAGG 
CAACATCTTC AGCGACTTCG TAGCTACAGC GCTGGAGAGG CCTCTGAAGT CTCTCGAAAC 
AGAGGAGTCA CCCTGTTGGC CAGACCAGGC AATAGTCTTG TAACAGCTAT TCGAAACCAG 
CACCATCATG AGGCATTACC TCTGGCTTAC AATGACAAGA TTGTTGCATT TTTACGCCAA 
CCCAACATTT TTGAGATGCT GCAGGAGCGT CAGCCCAGCT TGGCCAGAAA CCATGCACTC 
AGGGAGAAGA TCCATTACAT TCGGACAGAG GGTACACATG GGCTTGAAAA ATTGTCCTGT 
GATGCAGATT TAGTAATTCT GCTGAGCCTT TTTGAAGAGG ATATTATGTC CTACATACCA 
CTGCAGGCTG CCTTCCATCC TGGTTACAGT TTCTCTCCTC GCTGCTCACC CTGTTCATCA 
CCCCAAAATT CCCCAGGGCT GCAGAGAGCA AGTGCAAGAG CACCTTCTCC ATACAGGAGG 
GACTTTGAAG CCAAGCTGCG CAATTTCTAT CGAAAACTTG AAGCCAAAGG GTATGGGCAA 
GGTCCAGGTA AAATTAAGTT AATTATACGG CGTGACCACT TGCTGGAAGG CACCTTCAAC 
CAGGTAATGG CATATTCACG CAAGGAGCTC CAGCGGAACA AACTCTACGT CACGTTTGTT 
GGCGAGGAAG GGTTGGACTA CAGTGGCCCC TCCCGAGAAT TCTTCTTCCT TCTGTCTCAG 
GAGCTCTTCA ATCCTTATTA TGGGCTGTTT GAGTATTCAG CCAATGACAC TTACACTGTA 
CAGATCAGCC CCATGTCTGC CTTTGTTGAA AATCACCTGG AATGGTTCAG GTTCAGCGGT 
CGTATTCTTG GCCTTGCTCT CATTCATCAG TACCTTCTTG ATGCTTTCTT CACAAGGCCG 
TTCTACAAAG CACTACTAAG GCTACCATGT GATTTAAGTG ACTTAGAGTA CCTGGATGAA 
GAGTTCCATC AGAGCTTGCA GTGGATGAAG GATAACAACA TCACAGATAT CTTGGACTTA 
ACCTTCACAG TGAACGAGGA GGTATTTGGA CAGGTGACAG AGAGAGAGTT GAAATCTGGT 
GGGGCAAATA CAGCAGTGAC AGAAAAGAAC AAAAAAGAGT ACATCGAGAG GATGGTTAAG 
TGGCGAGTGG AACGAGGAGT GGTTCAGCAA ACAGAGGCCC TGGTCCGTGG CTTCTATGAA 
GTTGTAGACT CCAGGCTCGT CTCTGTTTTT GATGCCAGAG AACTGGAACT GGTTATAGCT 
GGCACTGCAG AAATAGATCT CAATGACTGG CGGAACAACA CAGAATATCG TGGAGGTTAC 
CATGATGGAC ACATAGTAAT ACGGTGGTTC TGGGCAGCAG TGGAGAGGTT TAACAACGAG 
CAGAGGCTCC GGCTGTTACA GTTCGTCACA GGAACATCCA GCGTGCCATA CGAGGGCTTT 
GCTGCTCTCC GAGGAAGCAA CGGGCTCCGG CGCTTCTGTA TAGAGAAATG GGGGAAAATA 
ACCTCCCTCC CAAGGGCACA TACATGTTTC AACCGTTTGG ATCTTCCACC TTACCCCTCA 
TATTCTATGC TGTATGAAAA GCTGCTGACA GCTGTTGAAG AAACTAGCAC CTTTGGACTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017823121.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678610.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLe49379
Clone ID Related Accession (Same CDS sequence)	XM_017823121.1
Accession Version	XM_017823121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAGCTT 
CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT 
ACAACATCCT TGGGAAACTT TACCTGGTGG AATTGGGAAG CTCGGATAGA CAGCCACGGG 
AGGGTATTCT ACGTTGATCA TGTCAACCGA ACCACCACGT GGCAGCGCCC CACAGCCGCG 
GCAACCCCGG ATGGGATCCA CAGGTCTGGC TCCATCCAGC AAATGGAGCA GCTGAACCGG 
CGATACCAAA ACATCCAGCG AACAATCGCA ACAGAGCGGA CTGACGATGA TGCTGTGGTA 
AACAACAGGG TGGAGAGAGT TCCCACTGGA GGAGGAAGTG ACTCTGAGGC AGAGCCTTCT 
CAGCCAAGCT CAGAGATTAG GAGGGAAGGT TCCCTTTCTC CTGTGAATTC TCAGAAAATC 
ACCTTGTTGC TGCAGTCTCC AGCTGTGAAG TTCATTACCA ATCCTGAGTT CTTCACTGTG 
CTGCATGCAA ACTATAGTGC CTATCGGGTC TTCACTAACA GTACCTGCTT GAAGCATATG 
ATTCTGAAGA TTCGTAGAGA TGCTCGTAAT TTTGAGCGGT ATCAGCACAA CAGAGACCTG 
GTAAATTTTA TCAACATGTT TGCTGATACC CGACTGGAGC TTCCTCGTGG CTGGGAGATA 
AAAACTGACC AGCAGGGCAA GTCTTTCTTC GTTGACCACA ATAGCCGTGC TACCACCTTC 
ATAGATCCCA GGATTCCGCT GCAGAATGGT CGTCTCCCTA ATCACCTGAC TCACAGGCAA 
CATCTTCAGC GACTTCGTAG CTACAGCGCT GGAGAGGCCT CTGAAGTCTC TCGAAACAGA 
GGAGTCACCC TGTTGGCCAG ACCAGGCAAT AGTCTTGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGG CATTACCTCT GGCTTACAAT GACAAGATTG TTGCATTTTT ACGCCAACCC 
AACATTTTTG AGATGCTGCA GGAGCGTCAG CCCAGCTTGG CCAGAAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAATT GTCCTGTGAT 
GCAGATTTAG TAATTCTGCT GAGCCTTTTT GAAGAGGATA TTATGTCCTA CATACCACTG 
CAGGCTGCCT TCCATCCTGG TTACAGTTTC TCTCCTCGCT GCTCACCCTG TTCATCACCC 
CAAAATTCCC CAGGGCTGCA GAGAGCAAGT GCAAGAGCAC CTTCTCCATA CAGGAGGGAC 
TTTGAAGCCA AGCTGCGCAA TTTCTATCGA AAACTTGAAG CCAAAGGGTA TGGGCAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGC TGGAAGGCAC CTTCAACCAG 
GTAATGGCAT ATTCACGCAA GGAGCTCCAG CGGAACAAAC TCTACGTCAC GTTTGTTGGC 
GAGGAAGGGT TGGACTACAG TGGCCCCTCC CGAGAATTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTATTATGG GCTGTTTGAG TATTCAGCCA ATGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCCTT TGTTGAAAAT CACCTGGAAT GGTTCAGGTT CAGCGGTCGT 
ATTCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TACAAAGCAC TACTAAGGCT ACCATGTGAT TTAAGTGACT TAGAGTACCT GGATGAAGAG 
TTCCATCAGA GCTTGCAGTG GATGAAGGAT AACAACATCA CAGATATCTT GGACTTAACC 
TTCACAGTGA ACGAGGAGGT ATTTGGACAG GTGACAGAGA GAGAGTTGAA ATCTGGTGGG 
GCAAATACAG CAGTGACAGA AAAGAACAAA AAAGAGTACA TCGAGAGGAT GGTTAAGTGG 
CGAGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCCCTGG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTCGTCTC TGTTTTTGAT GCCAGAGAAC TGGAACTGGT TATAGCTGGC 
ACTGCAGAAA TAGATCTCAA TGACTGGCGG AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGACACA TAGTAATACG GTGGTTCTGG GCAGCAGTGG AGAGGTTTAA CAACGAGCAG 
AGGCTCCGGC TGTTACAGTT CGTCACAGGA ACATCCAGCG TGCCATACGA GGGCTTTGCT 
GCTCTCCGAG GAAGCAACGG GCTCCGGCGC TTCTGTATAG AGAAATGGGG GAAAATAACC 
TCCCTCCCAA GGGCACATAC ATGTTTCAAC CGTTTGGATC TTCCACCTTA CCCCTCATAT 
TCTATGCTGT ATGAAAAGCT GCTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678610.1
CDS	165..5027
Translation	MSCAQAGIVQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPTTTSLGNFTWWNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823121.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823121.1

ORF Insert Sequence:

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAGCTT 
CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT 
ACAACATCCT TGGGAAACTT TACCTGGTGG AATTGGGAAG CTCGGATAGA CAGCCACGGG 
AGGGTATTCT ACGTTGATCA TGTCAACCGA ACCACCACGT GGCAGCGCCC CACAGCCGCG 
GCAACCCCGG ATGGGATCCA CAGGTCTGGC TCCATCCAGC AAATGGAGCA GCTGAACCGG 
CGATACCAAA ACATCCAGCG AACAATCGCA ACAGAGCGGA CTGACGATGA TGCTGTGGTA 
AACAACAGGG TGGAGAGAGT TCCCACTGGA GGAGGAAGTG ACTCTGAGGC AGAGCCTTCT 
CAGCCAAGCT CAGAGATTAG GAGGGAAGGT TCCCTTTCTC CTGTGAATTC TCAGAAAATC 
ACCTTGTTGC TGCAGTCTCC AGCTGTGAAG TTCATTACCA ATCCTGAGTT CTTCACTGTG 
CTGCATGCAA ACTATAGTGC CTATCGGGTC TTCACTAACA GTACCTGCTT GAAGCATATG 
ATTCTGAAGA TTCGTAGAGA TGCTCGTAAT TTTGAGCGGT ATCAGCACAA CAGAGACCTG 
GTAAATTTTA TCAACATGTT TGCTGATACC CGACTGGAGC TTCCTCGTGG CTGGGAGATA 
AAAACTGACC AGCAGGGCAA GTCTTTCTTC GTTGACCACA ATAGCCGTGC TACCACCTTC 
ATAGATCCCA GGATTCCGCT GCAGAATGGT CGTCTCCCTA ATCACCTGAC TCACAGGCAA 
CATCTTCAGC GACTTCGTAG CTACAGCGCT GGAGAGGCCT CTGAAGTCTC TCGAAACAGA 
GGAGTCACCC TGTTGGCCAG ACCAGGCAAT AGTCTTGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGG CATTACCTCT GGCTTACAAT GACAAGATTG TTGCATTTTT ACGCCAACCC 
AACATTTTTG AGATGCTGCA GGAGCGTCAG CCCAGCTTGG CCAGAAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAATT GTCCTGTGAT 
GCAGATTTAG TAATTCTGCT GAGCCTTTTT GAAGAGGATA TTATGTCCTA CATACCACTG 
CAGGCTGCCT TCCATCCTGG TTACAGTTTC TCTCCTCGCT GCTCACCCTG TTCATCACCC 
CAAAATTCCC CAGGGCTGCA GAGAGCAAGT GCAAGAGCAC CTTCTCCATA CAGGAGGGAC 
TTTGAAGCCA AGCTGCGCAA TTTCTATCGA AAACTTGAAG CCAAAGGGTA TGGGCAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGC TGGAAGGCAC CTTCAACCAG 
GTAATGGCAT ATTCACGCAA GGAGCTCCAG CGGAACAAAC TCTACGTCAC GTTTGTTGGC 
GAGGAAGGGT TGGACTACAG TGGCCCCTCC CGAGAATTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTATTATGG GCTGTTTGAG TATTCAGCCA ATGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCCTT TGTTGAAAAT CACCTGGAAT GGTTCAGGTT CAGCGGTCGT 
ATTCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TACAAAGCAC TACTAAGGCT ACCATGTGAT TTAAGTGACT TAGAGTACCT GGATGAAGAG 
TTCCATCAGA GCTTGCAGTG GATGAAGGAT AACAACATCA CAGATATCTT GGACTTAACC 
TTCACAGTGA ACGAGGAGGT ATTTGGACAG GTGACAGAGA GAGAGTTGAA ATCTGGTGGG 
GCAAATACAG CAGTGACAGA AAAGAACAAA AAAGAGTACA TCGAGAGGAT GGTTAAGTGG 
CGAGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCCCTGG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTCGTCTC TGTTTTTGAT GCCAGAGAAC TGGAACTGGT TATAGCTGGC 
ACTGCAGAAA TAGATCTCAA TGACTGGCGG AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGACACA TAGTAATACG GTGGTTCTGG GCAGCAGTGG AGAGGTTTAA CAACGAGCAG 
AGGCTCCGGC TGTTACAGTT CGTCACAGGA ACATCCAGCG TGCCATACGA GGGCTTTGCT 
GCTCTCCGAG GAAGCAACGG GCTCCGGCGC TTCTGTATAG AGAAATGGGG GAAAATAACC 
TCCCTCCCAA GGGCACATAC ATGTTTCAAC CGTTTGGATC TTCCACCTTA CCCCTCATAT 
TCTATGCTGT ATGAAAAGCT GCTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017823131.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678620.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OLe49380
Clone ID Related Accession (Same CDS sequence)	XM_017823131.1
Accession Version	XM_017823131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT GGGCCTGGCA 
GCCATGGCGT CTCCATCTAG AAACTCACAA AATCGACGCC GGTGCAAAGA GCCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTCCATAAC ATGGACATCA GGAACAATTC CCATGAGACA 
GTCACCATTC CTAGGTCTAC CAGTGACACA GACCTTGTCA CTTCAGACAG CCGGTCCACC 
CTGATGGTCA GCAGCTCCTA CTACTCCATT GGGCATTCGC AGGACCTAGT GATATACTGG 
GATATAAAAG AGGAAGTGGA TGCAGGGGAC TGGATTGGCA TGTATCTCAT AGATGAGGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAGAAC CGCGGTGTCA ATGGCTCTCA CCGTGGACAG 
ATTATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAGC CTGAAACAAA GATCTGCTTC 
AAGTACTACC ATGGTGTGAG TGGGGCCCTA CGAGCCACAA CTCCAAGTGT CACAGTGAAA 
AACAATGCAG CTCCTCAGAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAGCTT 
CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT 
ACAACATCCT TGGGAAACTT TACCTGGTGG AATTGGGAAG CTCGGATAGA CAGCCACGGG 
AGGGTATTCT ACGTTGATCA TGTCAACCGA ACCACCACGT GGCAGCGCCC CACAGCCGCG 
GCAACCCCGG ATGGGATCCA CAGGTCTGGC TCCATCCAGC AAATGGAGCA GCTGAACCGG 
CGATACCAAA ACATCCAGCG AACAATCGCA ACAGAGCGGA CTGACGATGA TGCTGTGGTA 
AACAACAGGG TGGAGAGAGT TCCCACTGGA GGAGGAAGTG ACTCTGAGGC AGAGCCTTCT 
CAGCCAAGCT CAGAGATTAG GAGGGAAGGT TCCCTTTCTC CTGTGAATTC TCAGAAAATC 
ACCTTGTTGC TGCAGTCTCC AGCTGTGAAG TTCATTACCA ATCCTGAGTT CTTCACTGTG 
CTGCATGCAA ACTATAGTGC CTATCGGGTC TTCACTAACA GTACCTGCTT GAAGCATATG 
ATTCTGAAGA TTCGTAGAGA TGCTCGTAAT TTTGAGCGGT ATCAGCACAA CAGAGACCTG 
GTAAATTTTA TCAACATGTT TGCTGATACC CGACTGGAGC TTCCTCGTGG CTGGGAGATA 
AAAACTGACC AGCAGGGCAA GTCTTTCTTC GTTGACCACA ATAGCCGTGC TACCACCTTC 
ATAGATCCCA GGATTCCGCT GCAGAATGGT CGTCTCCCTA ATCACCTGAC TCACAGGCAA 
CATCTTCAGC GACTTCGTAG CTACAGCGCT GGAGAGGCCT CTGAAGTCTC TCGAAACAGA 
GGAGTCACCC TGTTGGCCAG ACCAGGCAAT AGTCTTGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGG CATTACCTCT GGCTTACAAT GACAAGATTG TTGCATTTTT ACGCCAACCC 
AACATTTTTG AGATGCTGCA GGAGCGTCAG CCCAGCTTGG CCAGAAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAATT GTCCTGTGAT 
GCAGATTTAG TAATTCTGCT GAGCCTTTTT GAAGAGGATA TTATGTCCTA CATACCACTG 
CAGGCTGCCT TCCATCCTGG TTACAGTTTC TCTCCTCGCT GCTCACCCTG TTCATCACCC 
CAAAATTCCC CAGGGCTGCA GAGAGCAAGT GCAAGAGCAC CTTCTCCATA CAGGAGGGAC 
TTTGAAGCCA AGCTGCGCAA TTTCTATCGA AAACTTGAAG CCAAAGGGTA TGGGCAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGC TGGAAGGCAC CTTCAACCAG 
GTAATGGCAT ATTCACGCAA GGAGCTCCAG CGGAACAAAC TCTACGTCAC GTTTGTTGGC 
GAGGAAGGGT TGGACTACAG TGGCCCCTCC CGAGAATTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTATTATGG GCTGTTTGAG TATTCAGCCA ATGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCCTT TGTTGAAAAT CACCTGGAAT GGTTCAGGTT CAGCGGTCGT 
ATTCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TACAAAGCAC TACTAAGGCT ACCATGTGAT TTAAGTGACT TAGAGTACCT GGATGAAGAG 
TTCCATCAGA GCTTGCAGTG GATGAAGGAT AACAACATCA CAGATATCTT GGACTTAACC 
TTCACAGTGA ACGAGGAGGT ATTTGGACAG GTGACAGAGA GAGAGTTGAA ATCTGGTGGG 
GCAAATACAG CAGTGACAGA AAAGAACAAA AAAGAGTACA TCGAGAGGAT GGTTAAGTGG 
CGAGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCCCTGG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTCGTCTC TGTTTTTGAT GCCAGAGAAC TGGAACTGGT TATAGCTGGC 
ACTGCAGAAA TAGATCTCAA TGACTGGCGG AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGACACA TAGTAATACG GTGGTTCTGG GCAGCAGTGG AGAGGTTTAA CAACGAGCAG 
AGGCTCCGGC TGTTACAGTT CGTCACAGGA ACATCCAGCG TGCCATACGA GGGCTTTGCT 
GCTCTCCGAG GAAGCAACGG GCTCCGGCGC TTCTGTATAG AGAAATGGGG GAAAATAACC 
TCCCTCCCAA GGGCACATAC ATGTTTCAAC CGTTTGGATC TTCCACCTTA CCCCTCATAT 
TCTATGCTGT ATGAAAAGCT GCTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678620.1
CDS	226..5088
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPQIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPTTTSLGNFTWWNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823131.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823131.1

ORF Insert Sequence:

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT GGGCCTGGCA 
GCCATGGCGT CTCCATCTAG AAACTCACAA AATCGACGCC GGTGCAAAGA GCCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTCCATAAC ATGGACATCA GGAACAATTC CCATGAGACA 
GTCACCATTC CTAGGTCTAC CAGTGACACA GACCTTGTCA CTTCAGACAG CCGGTCCACC 
CTGATGGTCA GCAGCTCCTA CTACTCCATT GGGCATTCGC AGGACCTAGT GATATACTGG 
GATATAAAAG AGGAAGTGGA TGCAGGGGAC TGGATTGGCA TGTATCTCAT AGATGAGGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAGAAC CGCGGTGTCA ATGGCTCTCA CCGTGGACAG 
ATTATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAGC CTGAAACAAA GATCTGCTTC 
AAGTACTACC ATGGTGTGAG TGGGGCCCTA CGAGCCACAA CTCCAAGTGT CACAGTGAAA 
AACAATGCAG CTCCTCAGAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAGCTT 
CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT 
ACAACATCCT TGGGAAACTT TACCTGGTGG AATTGGGAAG CTCGGATAGA CAGCCACGGG 
AGGGTATTCT ACGTTGATCA TGTCAACCGA ACCACCACGT GGCAGCGCCC CACAGCCGCG 
GCAACCCCGG ATGGGATCCA CAGGTCTGGC TCCATCCAGC AAATGGAGCA GCTGAACCGG 
CGATACCAAA ACATCCAGCG AACAATCGCA ACAGAGCGGA CTGACGATGA TGCTGTGGTA 
AACAACAGGG TGGAGAGAGT TCCCACTGGA GGAGGAAGTG ACTCTGAGGC AGAGCCTTCT 
CAGCCAAGCT CAGAGATTAG GAGGGAAGGT TCCCTTTCTC CTGTGAATTC TCAGAAAATC 
ACCTTGTTGC TGCAGTCTCC AGCTGTGAAG TTCATTACCA ATCCTGAGTT CTTCACTGTG 
CTGCATGCAA ACTATAGTGC CTATCGGGTC TTCACTAACA GTACCTGCTT GAAGCATATG 
ATTCTGAAGA TTCGTAGAGA TGCTCGTAAT TTTGAGCGGT ATCAGCACAA CAGAGACCTG 
GTAAATTTTA TCAACATGTT TGCTGATACC CGACTGGAGC TTCCTCGTGG CTGGGAGATA 
AAAACTGACC AGCAGGGCAA GTCTTTCTTC GTTGACCACA ATAGCCGTGC TACCACCTTC 
ATAGATCCCA GGATTCCGCT GCAGAATGGT CGTCTCCCTA ATCACCTGAC TCACAGGCAA 
CATCTTCAGC GACTTCGTAG CTACAGCGCT GGAGAGGCCT CTGAAGTCTC TCGAAACAGA 
GGAGTCACCC TGTTGGCCAG ACCAGGCAAT AGTCTTGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGG CATTACCTCT GGCTTACAAT GACAAGATTG TTGCATTTTT ACGCCAACCC 
AACATTTTTG AGATGCTGCA GGAGCGTCAG CCCAGCTTGG CCAGAAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAATT GTCCTGTGAT 
GCAGATTTAG TAATTCTGCT GAGCCTTTTT GAAGAGGATA TTATGTCCTA CATACCACTG 
CAGGCTGCCT TCCATCCTGG TTACAGTTTC TCTCCTCGCT GCTCACCCTG TTCATCACCC 
CAAAATTCCC CAGGGCTGCA GAGAGCAAGT GCAAGAGCAC CTTCTCCATA CAGGAGGGAC 
TTTGAAGCCA AGCTGCGCAA TTTCTATCGA AAACTTGAAG CCAAAGGGTA TGGGCAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGC TGGAAGGCAC CTTCAACCAG 
GTAATGGCAT ATTCACGCAA GGAGCTCCAG CGGAACAAAC TCTACGTCAC GTTTGTTGGC 
GAGGAAGGGT TGGACTACAG TGGCCCCTCC CGAGAATTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTATTATGG GCTGTTTGAG TATTCAGCCA ATGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCCTT TGTTGAAAAT CACCTGGAAT GGTTCAGGTT CAGCGGTCGT 
ATTCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TACAAAGCAC TACTAAGGCT ACCATGTGAT TTAAGTGACT TAGAGTACCT GGATGAAGAG 
TTCCATCAGA GCTTGCAGTG GATGAAGGAT AACAACATCA CAGATATCTT GGACTTAACC 
TTCACAGTGA ACGAGGAGGT ATTTGGACAG GTGACAGAGA GAGAGTTGAA ATCTGGTGGG 
GCAAATACAG CAGTGACAGA AAAGAACAAA AAAGAGTACA TCGAGAGGAT GGTTAAGTGG 
CGAGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCCCTGG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTCGTCTC TGTTTTTGAT GCCAGAGAAC TGGAACTGGT TATAGCTGGC 
ACTGCAGAAA TAGATCTCAA TGACTGGCGG AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGACACA TAGTAATACG GTGGTTCTGG GCAGCAGTGG AGAGGTTTAA CAACGAGCAG 
AGGCTCCGGC TGTTACAGTT CGTCACAGGA ACATCCAGCG TGCCATACGA GGGCTTTGCT 
GCTCTCCGAG GAAGCAACGG GCTCCGGCGC TTCTGTATAG AGAAATGGGG GAAAATAACC 
TCCCTCCCAA GGGCACATAC ATGTTTCAAC CGTTTGGATC TTCCACCTTA CCCCTCATAT 
TCTATGCTGT ATGAAAAGCT GCTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017823140.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678629.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OLe49381
Clone ID Related Accession (Same CDS sequence)	XM_017823140.1
Accession Version	XM_017823140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGACTC AATCCAGGGA 
CAAGGAAATC GGAGACTGAT AAGTTTTTCA TTGTCAGATT TCCAGGCCTT TGGTCTGAAG 
AAAGGAATGT TCTTTAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGAAAACAT 
AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GATCTAAGAT CATGTGTAAT 
ACCGTCAACC CGATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCCCTGCC CACGGACGTC 
CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA ACGTTTTCTG 
GGAAAACTTT CTATGCCGGT TCAACGCCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT 
GTAAGCTACA CTCTGGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTT 
CGATTTGAAA TAACTTCTTC CATCCATCCA GATGATGAAG AGGTCTCCAT TAGTGCAGAT 
CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAATC CCACAGAGGA AAGCCTCGGT 
GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAGTGCAG CTCCAGAACA GCTAAATGGA 
TACAGTGTGA ACAGTGTCGA TAGCCCAGGG GCTGTCAAAC CACCTGGGGA AGTCAACCAG 
AACAGCTTAA ATGAAGAGCT AGCAGGAGAT GGGGAGGTTG ATGCGGTGCC AGTTCCTGAA 
CCCTTGGAAT CCATCCCAGG AGAAATGCTT TCTTCTTTGA GTGCTACGGA CCTCACAGAG 
CAGTCGACTC TGATGGCTTG CATGTCTCCT CCTGAGACTG AAGTTAGGGA AAATAACAAA 
GTCAATTCTG GTATTCAGGG AGATGGTGGA GTGACCAGCA TAGAAACATT AGATATTGAT 
GAGGTGAGTG CAGATGTGCG TGCTGGCAAG GACTTAGAGA AAAGTCAGGA AGAAGATGAA 
GGGGCTTCAG CCCAGGAGGA GGAAGACAAC AAGCTACAAC TGAGGACCAC AGCAAAGAGG 
AAAAACAGGC CGTGCTCCCT GCCTGTGTCT GAACTTGAGA CGGTCATAGC TTCAGCCTGC 
GGTGAGCCAG AGACCCCTCG CACACACTAC ATACGGATCC ACAACCTACT CCACAGCCTG 
CCCTCGGCAC AGATGAGCAG CGATGGGGAA GAAGAACAAG ATGGTGGCAA TGGCGGGGAG 
AACGATGAGG AATCTACAGT CAAGGAGGTG TTGGATAAGG AAGTGGTCAG TGAGAGTGAG 
ACCGTGGCAG CAGAGCCTCC TCTGGAAAAT GAGGCTGAAG AGAACTTCAG CCAGAGAACA 
GTGCCTCCTG AGACCTTGGA TGAGCAACTC TCTGACTTAA ATGATGGGCT GTTGGATGGG 
GAGCCCCTCA GGACAGGAAG CGAGAGCGAG TCAAGCTCCA GGCCCAATGG CGACAACGAG 
TGCGAAGCGT GCGACACCTC TTGCTACAGC CCCTCTTGCT ACAGCACTTC CTGCTACAGC 
ACTTCCTGCT ACAGCACTTC TTGCTACAGC ACCTCGTGTT ATGACAGTAA CAATCGCTTC 
TCCAGCCATA CTCGCTTCTC CTCCGTCGAC AGCGCAAAGA TTTCTGAGAG CACAGTCTTT 
TCCTCACAGG ATGATGATGA GGAGGAGAAC AGTGCTTTTG AGTCAGTGCC AGATTCAGTG 
CAGAGCCCTG AGCTGGACAG GGAGCAAGTG GATGGCTCCT CCCAGTGGCC AGATGAACTA 
GTAGCTCATG CTGGGAATCC ACAAAGAGCC ACAGAGGGTC TAGACACCCC AATAGCAGGT 
CCGAGCAGCA GAAGAGAAGG TGATTGTCCT TTACTCCATA ATTCTCAGCC AGTCAGCCAG 
CTTCCTTCTC TGAGGCCTGA CCATCATCAC TACCCAACTA TCGATGAGCC TCTTCCACCA 
AATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTATTCT ACGTTGATCA TGTCAACCGA 
ACCACCACGT GGCAGCGCCC CACAGCCGCG GCAACCCCGG ATGGGATCCA CAGGTCTGGC 
TCCATCCAGC AAATGGAGCA GCTGAACCGG CGATACCAAA ACATCCAGCG AACAATCGCA 
ACAGAGCGGA CTGACGATGA TGCTGTGGTA AACAACAGGG TGGAGAGAGT TCCCACTGGA 
GGAGGAAGTG ACTCTGAGGC AGAGCCTTCT CAGCCAAGCT CAGAGATTAG GAGGGAAGGT 
TCCCTTTCTC CTGTGAATTC TCAGAAAATC ACCTTGTTGC TGCAGTCTCC AGCTGTGAAG 
TTCATTACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGGGTC 
TTCACTAACA GTACCTGCTT GAAGCATATG ATTCTGAAGA TTCGTAGAGA TGCTCGTAAT 
TTTGAGCGGT ATCAGCACAA CAGAGACCTG GTAAATTTTA TCAACATGTT TGCTGATACC 
CGACTGGAGC TTCCTCGTGG CTGGGAGATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC 
GTTGACCACA ATAGCCGTGC TACCACCTTC ATAGATCCCA GGATTCCGCT GCAGAATGGT 
CGTCTCCCTA ATCACCTGAC TCACAGGCAA CATCTTCAGC GACTTCGTAG CTACAGCGCT 
GGAGAGGCCT CTGAAGTCTC TCGAAACAGA GGAGTCACCC TGTTGGCCAG ACCAGGCAAT 
AGTCTTGTAA CAGCTATTCG AAACCAGCAC CATCATGAGG CATTACCTCT GGCTTACAAT 
GACAAGATTG TTGCATTTTT ACGCCAACCC AACATTTTTG AGATGCTGCA GGAGCGTCAG 
CCCAGCTTGG CCAGAAACCA TGCACTCAGG GAGAAGATCC ATTACATTCG GACAGAGGGT 
ACACATGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT 
GAAGAGGATA TTATGTCCTA CATACCACTG CAGGCTGCCT TCCATCCTGG TTACAGTTTC 
TCTCCTCGCT GCTCACCCTG TTCATCACCC CAAAATTCCC CAGGGCTGCA GAGAGCAAGT 
GCAAGAGCAC CTTCTCCATA CAGGAGGGAC TTTGAAGCCA AGCTGCGCAA TTTCTATCGA 
AAACTTGAAG CCAAAGGGTA TGGGCAAGGT CCAGGTAAAA TTAAGTTAAT TATACGGCGT 
GACCACTTGC TGGAAGGCAC CTTCAACCAG GTAATGGCAT ATTCACGCAA GGAGCTCCAG 
CGGAACAAAC TCTACGTCAC GTTTGTTGGC GAGGAAGGGT TGGACTACAG TGGCCCCTCC 
CGAGAATTCT TCTTCCTTCT GTCTCAGGAG CTCTTCAATC CTTATTATGG GCTGTTTGAG 
TATTCAGCCA ATGACACTTA CACTGTACAG ATCAGCCCCA TGTCTGCCTT TGTTGAAAAT 
CACCTGGAAT GGTTCAGGTT CAGCGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC 
CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TACTAAGGCT ACCATGTGAT 
TTAAGTGACT TAGAGTACCT GGATGAAGAG TTCCATCAGA GCTTGCAGTG GATGAAGGAT 
AACAACATCA CAGATATCTT GGACTTAACC TTCACAGTGA ACGAGGAGGT ATTTGGACAG 
GTGACAGAGA GAGAGTTGAA ATCTGGTGGG GCAAATACAG CAGTGACAGA AAAGAACAAA 
AAAGAGTACA TCGAGAGGAT GGTTAAGTGG CGAGTGGAAC GAGGAGTGGT TCAGCAAACA 
GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCCA GGCTCGTCTC TGTTTTTGAT 
GCCAGAGAAC TGGAACTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGG 
AACAACACAG AATATCGTGG AGGTTACCAT GATGGACACA TAGTAATACG GTGGTTCTGG 
GCAGCAGTGG AGAGGTTTAA CAACGAGCAG AGGCTCCGGC TGTTACAGTT CGTCACAGGA 
ACATCCAGCG TGCCATACGA GGGCTTTGCT GCTCTCCGAG GAAGCAACGG GCTCCGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTCCCAA GGGCACATAC ATGTTTCAAC 
CGTTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GCTGACAGCT 
GTTGAAGAAA CTAGCACCTT TGGACTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678629.1
CDS	165..4997
Translation	MSCAQAGIVQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPQIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823140.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823140.1

ORF Insert Sequence:

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGACTC AATCCAGGGA 
CAAGGAAATC GGAGACTGAT AAGTTTTTCA TTGTCAGATT TCCAGGCCTT TGGTCTGAAG 
AAAGGAATGT TCTTTAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGAAAACAT 
AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GATCTAAGAT CATGTGTAAT 
ACCGTCAACC CGATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCCCTGCC CACGGACGTC 
CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA ACGTTTTCTG 
GGAAAACTTT CTATGCCGGT TCAACGCCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT 
GTAAGCTACA CTCTGGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTT 
CGATTTGAAA TAACTTCTTC CATCCATCCA GATGATGAAG AGGTCTCCAT TAGTGCAGAT 
CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAATC CCACAGAGGA AAGCCTCGGT 
GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAGTGCAG CTCCAGAACA GCTAAATGGA 
TACAGTGTGA ACAGTGTCGA TAGCCCAGGG GCTGTCAAAC CACCTGGGGA AGTCAACCAG 
AACAGCTTAA ATGAAGAGCT AGCAGGAGAT GGGGAGGTTG ATGCGGTGCC AGTTCCTGAA 
CCCTTGGAAT CCATCCCAGG AGAAATGCTT TCTTCTTTGA GTGCTACGGA CCTCACAGAG 
CAGTCGACTC TGATGGCTTG CATGTCTCCT CCTGAGACTG AAGTTAGGGA AAATAACAAA 
GTCAATTCTG GTATTCAGGG AGATGGTGGA GTGACCAGCA TAGAAACATT AGATATTGAT 
GAGGTGAGTG CAGATGTGCG TGCTGGCAAG GACTTAGAGA AAAGTCAGGA AGAAGATGAA 
GGGGCTTCAG CCCAGGAGGA GGAAGACAAC AAGCTACAAC TGAGGACCAC AGCAAAGAGG 
AAAAACAGGC CGTGCTCCCT GCCTGTGTCT GAACTTGAGA CGGTCATAGC TTCAGCCTGC 
GGTGAGCCAG AGACCCCTCG CACACACTAC ATACGGATCC ACAACCTACT CCACAGCCTG 
CCCTCGGCAC AGATGAGCAG CGATGGGGAA GAAGAACAAG ATGGTGGCAA TGGCGGGGAG 
AACGATGAGG AATCTACAGT CAAGGAGGTG TTGGATAAGG AAGTGGTCAG TGAGAGTGAG 
ACCGTGGCAG CAGAGCCTCC TCTGGAAAAT GAGGCTGAAG AGAACTTCAG CCAGAGAACA 
GTGCCTCCTG AGACCTTGGA TGAGCAACTC TCTGACTTAA ATGATGGGCT GTTGGATGGG 
GAGCCCCTCA GGACAGGAAG CGAGAGCGAG TCAAGCTCCA GGCCCAATGG CGACAACGAG 
TGCGAAGCGT GCGACACCTC TTGCTACAGC CCCTCTTGCT ACAGCACTTC CTGCTACAGC 
ACTTCCTGCT ACAGCACTTC TTGCTACAGC ACCTCGTGTT ATGACAGTAA CAATCGCTTC 
TCCAGCCATA CTCGCTTCTC CTCCGTCGAC AGCGCAAAGA TTTCTGAGAG CACAGTCTTT 
TCCTCACAGG ATGATGATGA GGAGGAGAAC AGTGCTTTTG AGTCAGTGCC AGATTCAGTG 
CAGAGCCCTG AGCTGGACAG GGAGCAAGTG GATGGCTCCT CCCAGTGGCC AGATGAACTA 
GTAGCTCATG CTGGGAATCC ACAAAGAGCC ACAGAGGGTC TAGACACCCC AATAGCAGGT 
CCGAGCAGCA GAAGAGAAGG TGATTGTCCT TTACTCCATA ATTCTCAGCC AGTCAGCCAG 
CTTCCTTCTC TGAGGCCTGA CCATCATCAC TACCCAACTA TCGATGAGCC TCTTCCACCA 
AATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTATTCT ACGTTGATCA TGTCAACCGA 
ACCACCACGT GGCAGCGCCC CACAGCCGCG GCAACCCCGG ATGGGATCCA CAGGTCTGGC 
TCCATCCAGC AAATGGAGCA GCTGAACCGG CGATACCAAA ACATCCAGCG AACAATCGCA 
ACAGAGCGGA CTGACGATGA TGCTGTGGTA AACAACAGGG TGGAGAGAGT TCCCACTGGA 
GGAGGAAGTG ACTCTGAGGC AGAGCCTTCT CAGCCAAGCT CAGAGATTAG GAGGGAAGGT 
TCCCTTTCTC CTGTGAATTC TCAGAAAATC ACCTTGTTGC TGCAGTCTCC AGCTGTGAAG 
TTCATTACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGGGTC 
TTCACTAACA GTACCTGCTT GAAGCATATG ATTCTGAAGA TTCGTAGAGA TGCTCGTAAT 
TTTGAGCGGT ATCAGCACAA CAGAGACCTG GTAAATTTTA TCAACATGTT TGCTGATACC 
CGACTGGAGC TTCCTCGTGG CTGGGAGATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC 
GTTGACCACA ATAGCCGTGC TACCACCTTC ATAGATCCCA GGATTCCGCT GCAGAATGGT 
CGTCTCCCTA ATCACCTGAC TCACAGGCAA CATCTTCAGC GACTTCGTAG CTACAGCGCT 
GGAGAGGCCT CTGAAGTCTC TCGAAACAGA GGAGTCACCC TGTTGGCCAG ACCAGGCAAT 
AGTCTTGTAA CAGCTATTCG AAACCAGCAC CATCATGAGG CATTACCTCT GGCTTACAAT 
GACAAGATTG TTGCATTTTT ACGCCAACCC AACATTTTTG AGATGCTGCA GGAGCGTCAG 
CCCAGCTTGG CCAGAAACCA TGCACTCAGG GAGAAGATCC ATTACATTCG GACAGAGGGT 
ACACATGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT 
GAAGAGGATA TTATGTCCTA CATACCACTG CAGGCTGCCT TCCATCCTGG TTACAGTTTC 
TCTCCTCGCT GCTCACCCTG TTCATCACCC CAAAATTCCC CAGGGCTGCA GAGAGCAAGT 
GCAAGAGCAC CTTCTCCATA CAGGAGGGAC TTTGAAGCCA AGCTGCGCAA TTTCTATCGA 
AAACTTGAAG CCAAAGGGTA TGGGCAAGGT CCAGGTAAAA TTAAGTTAAT TATACGGCGT 
GACCACTTGC TGGAAGGCAC CTTCAACCAG GTAATGGCAT ATTCACGCAA GGAGCTCCAG 
CGGAACAAAC TCTACGTCAC GTTTGTTGGC GAGGAAGGGT TGGACTACAG TGGCCCCTCC 
CGAGAATTCT TCTTCCTTCT GTCTCAGGAG CTCTTCAATC CTTATTATGG GCTGTTTGAG 
TATTCAGCCA ATGACACTTA CACTGTACAG ATCAGCCCCA TGTCTGCCTT TGTTGAAAAT 
CACCTGGAAT GGTTCAGGTT CAGCGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC 
CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TACTAAGGCT ACCATGTGAT 
TTAAGTGACT TAGAGTACCT GGATGAAGAG TTCCATCAGA GCTTGCAGTG GATGAAGGAT 
AACAACATCA CAGATATCTT GGACTTAACC TTCACAGTGA ACGAGGAGGT ATTTGGACAG 
GTGACAGAGA GAGAGTTGAA ATCTGGTGGG GCAAATACAG CAGTGACAGA AAAGAACAAA 
AAAGAGTACA TCGAGAGGAT GGTTAAGTGG CGAGTGGAAC GAGGAGTGGT TCAGCAAACA 
GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCCA GGCTCGTCTC TGTTTTTGAT 
GCCAGAGAAC TGGAACTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGG 
AACAACACAG AATATCGTGG AGGTTACCAT GATGGACACA TAGTAATACG GTGGTTCTGG 
GCAGCAGTGG AGAGGTTTAA CAACGAGCAG AGGCTCCGGC TGTTACAGTT CGTCACAGGA 
ACATCCAGCG TGCCATACGA GGGCTTTGCT GCTCTCCGAG GAAGCAACGG GCTCCGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTCCCAA GGGCACATAC ATGTTTCAAC 
CGTTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GCTGACAGCT 
GTTGAAGAAA CTAGCACCTT TGGACTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017823150.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678639.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

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CloneID	OLe49382
Clone ID Related Accession (Same CDS sequence)	XM_017823150.1
Accession Version	XM_017823150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAGCTT 
CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAAAT 
TGGGAAGCTC GGATAGACAG CCACGGGAGG GTATTCTACG TTGATCATGT CAACCGAACC 
ACCACGTGGC AGCGCCCCAC AGCCGCGGCA ACCCCGGATG GGATCCACAG GTCTGGCTCC 
ATCCAGCAAA TGGAGCAGCT GAACCGGCGA TACCAAAACA TCCAGCGAAC AATCGCAACA 
GAGCGGACTG ACGATGATGC TGTGGTAAAC AACAGGGTGG AGAGAGTTCC CACTGGAGGA 
GGAAGTGACT CTGAGGCAGA GCCTTCTCAG CCAAGCTCAG AGATTAGGAG GGAAGGTTCC 
CTTTCTCCTG TGAATTCTCA GAAAATCACC TTGTTGCTGC AGTCTCCAGC TGTGAAGTTC 
ATTACCAATC CTGAGTTCTT CACTGTGCTG CATGCAAACT ATAGTGCCTA TCGGGTCTTC 
ACTAACAGTA CCTGCTTGAA GCATATGATT CTGAAGATTC GTAGAGATGC TCGTAATTTT 
GAGCGGTATC AGCACAACAG AGACCTGGTA AATTTTATCA ACATGTTTGC TGATACCCGA 
CTGGAGCTTC CTCGTGGCTG GGAGATAAAA ACTGACCAGC AGGGCAAGTC TTTCTTCGTT 
GACCACAATA GCCGTGCTAC CACCTTCATA GATCCCAGGA TTCCGCTGCA GAATGGTCGT 
CTCCCTAATC ACCTGACTCA CAGGCAACAT CTTCAGCGAC TTCGTAGCTA CAGCGCTGGA 
GAGGCCTCTG AAGTCTCTCG AAACAGAGGA GTCACCCTGT TGGCCAGACC AGGCAATAGT 
CTTGTAACAG CTATTCGAAA CCAGCACCAT CATGAGGCAT TACCTCTGGC TTACAATGAC 
AAGATTGTTG CATTTTTACG CCAACCCAAC ATTTTTGAGA TGCTGCAGGA GCGTCAGCCC 
AGCTTGGCCA GAAACCATGC ACTCAGGGAG AAGATCCATT ACATTCGGAC AGAGGGTACA 
CATGGGCTTG AAAAATTGTC CTGTGATGCA GATTTAGTAA TTCTGCTGAG CCTTTTTGAA 
GAGGATATTA TGTCCTACAT ACCACTGCAG GCTGCCTTCC ATCCTGGTTA CAGTTTCTCT 
CCTCGCTGCT CACCCTGTTC ATCACCCCAA AATTCCCCAG GGCTGCAGAG AGCAAGTGCA 
AGAGCACCTT CTCCATACAG GAGGGACTTT GAAGCCAAGC TGCGCAATTT CTATCGAAAA 
CTTGAAGCCA AAGGGTATGG GCAAGGTCCA GGTAAAATTA AGTTAATTAT ACGGCGTGAC 
CACTTGCTGG AAGGCACCTT CAACCAGGTA ATGGCATATT CACGCAAGGA GCTCCAGCGG 
AACAAACTCT ACGTCACGTT TGTTGGCGAG GAAGGGTTGG ACTACAGTGG CCCCTCCCGA 
GAATTCTTCT TCCTTCTGTC TCAGGAGCTC TTCAATCCTT ATTATGGGCT GTTTGAGTAT 
TCAGCCAATG ACACTTACAC TGTACAGATC AGCCCCATGT CTGCCTTTGT TGAAAATCAC 
CTGGAATGGT TCAGGTTCAG CGGTCGTATT CTTGGCCTTG CTCTCATTCA TCAGTACCTT 
CTTGATGCTT TCTTCACAAG GCCGTTCTAC AAAGCACTAC TAAGGCTACC ATGTGATTTA 
AGTGACTTAG AGTACCTGGA TGAAGAGTTC CATCAGAGCT TGCAGTGGAT GAAGGATAAC 
AACATCACAG ATATCTTGGA CTTAACCTTC ACAGTGAACG AGGAGGTATT TGGACAGGTG 
ACAGAGAGAG AGTTGAAATC TGGTGGGGCA AATACAGCAG TGACAGAAAA GAACAAAAAA 
GAGTACATCG AGAGGATGGT TAAGTGGCGA GTGGAACGAG GAGTGGTTCA GCAAACAGAG 
GCCCTGGTCC GTGGCTTCTA TGAAGTTGTA GACTCCAGGC TCGTCTCTGT TTTTGATGCC 
AGAGAACTGG AACTGGTTAT AGCTGGCACT GCAGAAATAG ATCTCAATGA CTGGCGGAAC 
AACACAGAAT ATCGTGGAGG TTACCATGAT GGACACATAG TAATACGGTG GTTCTGGGCA 
GCAGTGGAGA GGTTTAACAA CGAGCAGAGG CTCCGGCTGT TACAGTTCGT CACAGGAACA 
TCCAGCGTGC CATACGAGGG CTTTGCTGCT CTCCGAGGAA GCAACGGGCT CCGGCGCTTC 
TGTATAGAGA AATGGGGGAA AATAACCTCC CTCCCAAGGG CACATACATG TTTCAACCGT 
TTGGATCTTC CACCTTACCC CTCATATTCT ATGCTGTATG AAAAGCTGCT GACAGCTGTT 
GAAGAAACTA GCACCTTTGG ACTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678639.1
CDS	165..4994
Translation	MSCAQAGIVQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823150.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823150.1

ORF Insert Sequence:

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAGCTT 
CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAAAT 
TGGGAAGCTC GGATAGACAG CCACGGGAGG GTATTCTACG TTGATCATGT CAACCGAACC 
ACCACGTGGC AGCGCCCCAC AGCCGCGGCA ACCCCGGATG GGATCCACAG GTCTGGCTCC 
ATCCAGCAAA TGGAGCAGCT GAACCGGCGA TACCAAAACA TCCAGCGAAC AATCGCAACA 
GAGCGGACTG ACGATGATGC TGTGGTAAAC AACAGGGTGG AGAGAGTTCC CACTGGAGGA 
GGAAGTGACT CTGAGGCAGA GCCTTCTCAG CCAAGCTCAG AGATTAGGAG GGAAGGTTCC 
CTTTCTCCTG TGAATTCTCA GAAAATCACC TTGTTGCTGC AGTCTCCAGC TGTGAAGTTC 
ATTACCAATC CTGAGTTCTT CACTGTGCTG CATGCAAACT ATAGTGCCTA TCGGGTCTTC 
ACTAACAGTA CCTGCTTGAA GCATATGATT CTGAAGATTC GTAGAGATGC TCGTAATTTT 
GAGCGGTATC AGCACAACAG AGACCTGGTA AATTTTATCA ACATGTTTGC TGATACCCGA 
CTGGAGCTTC CTCGTGGCTG GGAGATAAAA ACTGACCAGC AGGGCAAGTC TTTCTTCGTT 
GACCACAATA GCCGTGCTAC CACCTTCATA GATCCCAGGA TTCCGCTGCA GAATGGTCGT 
CTCCCTAATC ACCTGACTCA CAGGCAACAT CTTCAGCGAC TTCGTAGCTA CAGCGCTGGA 
GAGGCCTCTG AAGTCTCTCG AAACAGAGGA GTCACCCTGT TGGCCAGACC AGGCAATAGT 
CTTGTAACAG CTATTCGAAA CCAGCACCAT CATGAGGCAT TACCTCTGGC TTACAATGAC 
AAGATTGTTG CATTTTTACG CCAACCCAAC ATTTTTGAGA TGCTGCAGGA GCGTCAGCCC 
AGCTTGGCCA GAAACCATGC ACTCAGGGAG AAGATCCATT ACATTCGGAC AGAGGGTACA 
CATGGGCTTG AAAAATTGTC CTGTGATGCA GATTTAGTAA TTCTGCTGAG CCTTTTTGAA 
GAGGATATTA TGTCCTACAT ACCACTGCAG GCTGCCTTCC ATCCTGGTTA CAGTTTCTCT 
CCTCGCTGCT CACCCTGTTC ATCACCCCAA AATTCCCCAG GGCTGCAGAG AGCAAGTGCA 
AGAGCACCTT CTCCATACAG GAGGGACTTT GAAGCCAAGC TGCGCAATTT CTATCGAAAA 
CTTGAAGCCA AAGGGTATGG GCAAGGTCCA GGTAAAATTA AGTTAATTAT ACGGCGTGAC 
CACTTGCTGG AAGGCACCTT CAACCAGGTA ATGGCATATT CACGCAAGGA GCTCCAGCGG 
AACAAACTCT ACGTCACGTT TGTTGGCGAG GAAGGGTTGG ACTACAGTGG CCCCTCCCGA 
GAATTCTTCT TCCTTCTGTC TCAGGAGCTC TTCAATCCTT ATTATGGGCT GTTTGAGTAT 
TCAGCCAATG ACACTTACAC TGTACAGATC AGCCCCATGT CTGCCTTTGT TGAAAATCAC 
CTGGAATGGT TCAGGTTCAG CGGTCGTATT CTTGGCCTTG CTCTCATTCA TCAGTACCTT 
CTTGATGCTT TCTTCACAAG GCCGTTCTAC AAAGCACTAC TAAGGCTACC ATGTGATTTA 
AGTGACTTAG AGTACCTGGA TGAAGAGTTC CATCAGAGCT TGCAGTGGAT GAAGGATAAC 
AACATCACAG ATATCTTGGA CTTAACCTTC ACAGTGAACG AGGAGGTATT TGGACAGGTG 
ACAGAGAGAG AGTTGAAATC TGGTGGGGCA AATACAGCAG TGACAGAAAA GAACAAAAAA 
GAGTACATCG AGAGGATGGT TAAGTGGCGA GTGGAACGAG GAGTGGTTCA GCAAACAGAG 
GCCCTGGTCC GTGGCTTCTA TGAAGTTGTA GACTCCAGGC TCGTCTCTGT TTTTGATGCC 
AGAGAACTGG AACTGGTTAT AGCTGGCACT GCAGAAATAG ATCTCAATGA CTGGCGGAAC 
AACACAGAAT ATCGTGGAGG TTACCATGAT GGACACATAG TAATACGGTG GTTCTGGGCA 
GCAGTGGAGA GGTTTAACAA CGAGCAGAGG CTCCGGCTGT TACAGTTCGT CACAGGAACA 
TCCAGCGTGC CATACGAGGG CTTTGCTGCT CTCCGAGGAA GCAACGGGCT CCGGCGCTTC 
TGTATAGAGA AATGGGGGAA AATAACCTCC CTCCCAAGGG CACATACATG TTTCAACCGT 
TTGGATCTTC CACCTTACCC CTCATATTCT ATGCTGTATG AAAAGCTGCT GACAGCTGTT 
GAAGAAACTA GCACCTTTGG ACTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017823159.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678648.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

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CloneID	OLe49383
Clone ID Related Accession (Same CDS sequence)	XM_017823159.1
Accession Version	XM_017823159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CATCTAGAAA CTCACAAAAT CGACGCCGGT GCAAAGAGCC TCTCAGATAC 
AGCTATAACC CTGACCAGTT CCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC 
ACCATTCCTA GGTCTACCAG TGACACAGAC CTTGTCACTT CAGACAGCCG GTCCACCCTG 
ATGGTCAGCA GCTCCTACTA CTCCATTGGG CATTCGCAGG ACCTAGTGAT ATACTGGGAT 
ATAAAAGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAGGTCTTG 
TCTGAAAACT TTCTGGACTA CAAGAACCGC GGTGTCAATG GCTCTCACCG TGGACAGATT 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAGCCTG AAACAAAGAT CTGCTTCAAG 
TACTACCATG GTGTGAGTGG GGCCCTACGA GCCACAACTC CAAGTGTCAC AGTGAAAAAC 
AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ACTCAATCCA GGGACAAGGA 
AATCGGAGAC TGATAAGTTT TTCATTGTCA GATTTCCAGG CCTTTGGTCT GAAGAAAGGA 
ATGTTCTTTA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGAAA ACATAGCATC 
TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGATCTA AGATCATGTG TAATACCGTC 
AACCCGATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCCC TGCCCACGGA CGTCCTGGAA 
ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAACGTTT TCTGGGAAAA 
CTTTCTATGC CGGTTCAACG CCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC 
TACACTCTGG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTTCGATTT 
GAAATAACTT CTTCCATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AATCCCACAG AGGAAAGCCT CGGTGAGCCT 
CCATGCATCA ACACAGTGAC CTCAGAAAGT GCAGCTCCAG AACAGCTAAA TGGATACAGT 
GTGAACAGTG TCGATAGCCC AGGGGCTGTC AAACCACCTG GGGAAGTCAA CCAGAACAGC 
TTAAATGAAG AGCTAGCAGG AGATGGGGAG GTTGATGCGG TGCCAGTTCC TGAACCCTTG 
GAATCCATCC CAGGAGAAAT GCTTTCTTCT TTGAGTGCTA CGGACCTCAC AGAGCAGTCG 
ACTCTGATGG CTTGCATGTC TCCTCCTGAG ACTGAAGTTA GGGAAAATAA CAAAGTCAAT 
TCTGGTATTC AGGGAGATGG TGGAGTGACC AGCATAGAAA CATTAGATAT TGATGAGGTG 
AGTGCAGATG TGCGTGCTGG CAAGGACTTA GAGAAAAGTC AGGAAGAAGA TGAAGGGGCT 
TCAGCCCAGG AGGAGGAAGA CAACAAGCTA CAACTGAGGA CCACAGCAAA GAGGAAAAAC 
AGGCCGTGCT CCCTGCCTGT GTCTGAACTT GAGACGGTCA TAGCTTCAGC CTGCGGTGAG 
CCAGAGACCC CTCGCACACA CTACATACGG ATCCACAACC TACTCCACAG CCTGCCCTCG 
GCACAGATGA GCAGCGATGG GGAAGAAGAA CAAGATGGTG GCAATGGCGG GGAGAACGAT 
GAGGAATCTA CAGTCAAGGA GGTGTTGGAT AAGGAAGTGG TCAGTGAGAG TGAGACCGTG 
GCAGCAGAGC CTCCTCTGGA AAATGAGGCT GAAGAGAACT TCAGCCAGAG AACAGTGCCT 
CCTGAGACCT TGGATGAGCA ACTCTCTGAC TTAAATGATG GGCTGTTGGA TGGGGAGCCC 
CTCAGGACAG GAAGCGAGAG CGAGTCAAGC TCCAGGCCCA ATGGCGACAA CGAGTGCGAA 
GCGTGCGACA CCTCTTGCTA CAGCCCCTCT TGCTACAGCA CTTCCTGCTA CAGCACTTCC 
TGCTACAGCA CTTCTTGCTA CAGCACCTCG TGTTATGACA GTAACAATCG CTTCTCCAGC 
CATACTCGCT TCTCCTCCGT CGACAGCGCA AAGATTTCTG AGAGCACAGT CTTTTCCTCA 
CAGGATGATG ATGAGGAGGA GAACAGTGCT TTTGAGTCAG TGCCAGATTC AGTGCAGAGC 
CCTGAGCTGG ACAGGGAGCA AGTGGATGGC TCCTCCCAGT GGCCAGATGA ACTAGTAGCT 
CATGCTGGGA ATCCACAAAG AGCCACAGAG GGTCTAGACA CCCCAATAGC AGGTCCGAGC 
AGCAGAAGAG AAGGTGATTG TCCTTTACTC CATAATTCTC AGCCAGTCAG CCAGCTTCCT 
TCTCTGAGGC CTGACCATCA TCACTACCCA ACTATCGATG AGCCTCTTCC ACCAACTACA 
ACATCCTTGG GAAACTTTAC CTGGTGGAAT TGGGAAGCTC GGATAGACAG CCACGGGAGG 
GTATTCTACG TTGATCATGT CAACCGAACC ACCACGTGGC AGCGCCCCAC AGCCGCGGCA 
ACCCCGGATG GGATCCACAG GTCTGGCTCC ATCCAGCAAA TGGAGCAGCT GAACCGGCGA 
TACCAAAACA TCCAGCGAAC AATCGCAACA GAGCGGACTG ACGATGATGC TGTGGTAAAC 
AACAGGGTGG AGAGAGTTCC CACTGGAGGA GGAAGTGACT CTGAGGCAGA GCCTTCTCAG 
CCAAGCTCAG AGATTAGGAG GGAAGGTTCC CTTTCTCCTG TGAATTCTCA GAAAATCACC 
TTGTTGCTGC AGTCTCCAGC TGTGAAGTTC ATTACCAATC CTGAGTTCTT CACTGTGCTG 
CATGCAAACT ATAGTGCCTA TCGGGTCTTC ACTAACAGTA CCTGCTTGAA GCATATGATT 
CTGAAGATTC GTAGAGATGC TCGTAATTTT GAGCGGTATC AGCACAACAG AGACCTGGTA 
AATTTTATCA ACATGTTTGC TGATACCCGA CTGGAGCTTC CTCGTGGCTG GGAGATAAAA 
ACTGACCAGC AGGGCAAGTC TTTCTTCGTT GACCACAATA GCCGTGCTAC CACCTTCATA 
GATCCCAGGA TTCCGCTGCA GAATGGTCGT CTCCCTAATC ACCTGACTCA CAGGCAACAT 
CTTCAGCGAC TTCGTAGCTA CAGCGCTGGA GAGGCCTCTG AAGTCTCTCG AAACAGAGGA 
GTCACCCTGT TGGCCAGACC AGGCAATAGT CTTGTAACAG CTATTCGAAA CCAGCACCAT 
CATGAGGCAT TACCTCTGGC TTACAATGAC AAGATTGTTG CATTTTTACG CCAACCCAAC 
ATTTTTGAGA TGCTGCAGGA GCGTCAGCCC AGCTTGGCCA GAAACCATGC ACTCAGGGAG 
AAGATCCATT ACATTCGGAC AGAGGGTACA CATGGGCTTG AAAAATTGTC CTGTGATGCA 
GATTTAGTAA TTCTGCTGAG CCTTTTTGAA GAGGATATTA TGTCCTACAT ACCACTGCAG 
GCTGCCTTCC ATCCTGGTTA CAGTTTCTCT CCTCGCTGCT CACCCTGTTC ATCACCCCAA 
AATTCCCCAG GGCTGCAGAG AGCAAGTGCA AGAGCACCTT CTCCATACAG GAGGGACTTT 
GAAGCCAAGC TGCGCAATTT CTATCGAAAA CTTGAAGCCA AAGGGTATGG GCAAGGTCCA 
GGTAAAATTA AGTTAATTAT ACGGCGTGAC CACTTGCTGG AAGGCACCTT CAACCAGGTA 
ATGGCATATT CACGCAAGGA GCTCCAGCGG AACAAACTCT ACGTCACGTT TGTTGGCGAG 
GAAGGGTTGG ACTACAGTGG CCCCTCCCGA GAATTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAATCCTT ATTATGGGCT GTTTGAGTAT TCAGCCAATG ACACTTACAC TGTACAGATC 
AGCCCCATGT CTGCCTTTGT TGAAAATCAC CTGGAATGGT TCAGGTTCAG CGGTCGTATT 
CTTGGCCTTG CTCTCATTCA TCAGTACCTT CTTGATGCTT TCTTCACAAG GCCGTTCTAC 
AAAGCACTAC TAAGGCTACC ATGTGATTTA AGTGACTTAG AGTACCTGGA TGAAGAGTTC 
CATCAGAGCT TGCAGTGGAT GAAGGATAAC AACATCACAG ATATCTTGGA CTTAACCTTC 
ACAGTGAACG AGGAGGTATT TGGACAGGTG ACAGAGAGAG AGTTGAAATC TGGTGGGGCA 
AATACAGCAG TGACAGAAAA GAACAAAAAA GAGTACATCG AGAGGATGGT TAAGTGGCGA 
GTGGAACGAG GAGTGGTTCA GCAAACAGAG GCCCTGGTCC GTGGCTTCTA TGAAGTTGTA 
GACTCCAGGC TCGTCTCTGT TTTTGATGCC AGAGAACTGG AACTGGTTAT AGCTGGCACT 
GCAGAAATAG ATCTCAATGA CTGGCGGAAC AACACAGAAT ATCGTGGAGG TTACCATGAT 
GGACACATAG TAATACGGTG GTTCTGGGCA GCAGTGGAGA GGTTTAACAA CGAGCAGAGG 
CTCCGGCTGT TACAGTTCGT CACAGGAACA TCCAGCGTGC CATACGAGGG CTTTGCTGCT 
CTCCGAGGAA GCAACGGGCT CCGGCGCTTC TGTATAGAGA AATGGGGGAA AATAACCTCC 
CTCCCAAGGG CACATACATG TTTCAACCGT TTGGATCTTC CACCTTACCC CTCATATTCT 
ATGCTGTATG AAAAGCTGCT GACAGCTGTT GAAGAAACTA GCACCTTTGG ACTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678648.1
CDS	58..4857
Translation	MASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPQIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPTTTSLGNFTWWNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823159.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823159.1

ORF Insert Sequence:

ATGGCGTCTC CATCTAGAAA CTCACAAAAT CGACGCCGGT GCAAAGAGCC TCTCAGATAC 
AGCTATAACC CTGACCAGTT CCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC 
ACCATTCCTA GGTCTACCAG TGACACAGAC CTTGTCACTT CAGACAGCCG GTCCACCCTG 
ATGGTCAGCA GCTCCTACTA CTCCATTGGG CATTCGCAGG ACCTAGTGAT ATACTGGGAT 
ATAAAAGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAGGTCTTG 
TCTGAAAACT TTCTGGACTA CAAGAACCGC GGTGTCAATG GCTCTCACCG TGGACAGATT 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAGCCTG AAACAAAGAT CTGCTTCAAG 
TACTACCATG GTGTGAGTGG GGCCCTACGA GCCACAACTC CAAGTGTCAC AGTGAAAAAC 
AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ACTCAATCCA GGGACAAGGA 
AATCGGAGAC TGATAAGTTT TTCATTGTCA GATTTCCAGG CCTTTGGTCT GAAGAAAGGA 
ATGTTCTTTA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGAAA ACATAGCATC 
TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGATCTA AGATCATGTG TAATACCGTC 
AACCCGATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCCC TGCCCACGGA CGTCCTGGAA 
ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAACGTTT TCTGGGAAAA 
CTTTCTATGC CGGTTCAACG CCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC 
TACACTCTGG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTTCGATTT 
GAAATAACTT CTTCCATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AATCCCACAG AGGAAAGCCT CGGTGAGCCT 
CCATGCATCA ACACAGTGAC CTCAGAAAGT GCAGCTCCAG AACAGCTAAA TGGATACAGT 
GTGAACAGTG TCGATAGCCC AGGGGCTGTC AAACCACCTG GGGAAGTCAA CCAGAACAGC 
TTAAATGAAG AGCTAGCAGG AGATGGGGAG GTTGATGCGG TGCCAGTTCC TGAACCCTTG 
GAATCCATCC CAGGAGAAAT GCTTTCTTCT TTGAGTGCTA CGGACCTCAC AGAGCAGTCG 
ACTCTGATGG CTTGCATGTC TCCTCCTGAG ACTGAAGTTA GGGAAAATAA CAAAGTCAAT 
TCTGGTATTC AGGGAGATGG TGGAGTGACC AGCATAGAAA CATTAGATAT TGATGAGGTG 
AGTGCAGATG TGCGTGCTGG CAAGGACTTA GAGAAAAGTC AGGAAGAAGA TGAAGGGGCT 
TCAGCCCAGG AGGAGGAAGA CAACAAGCTA CAACTGAGGA CCACAGCAAA GAGGAAAAAC 
AGGCCGTGCT CCCTGCCTGT GTCTGAACTT GAGACGGTCA TAGCTTCAGC CTGCGGTGAG 
CCAGAGACCC CTCGCACACA CTACATACGG ATCCACAACC TACTCCACAG CCTGCCCTCG 
GCACAGATGA GCAGCGATGG GGAAGAAGAA CAAGATGGTG GCAATGGCGG GGAGAACGAT 
GAGGAATCTA CAGTCAAGGA GGTGTTGGAT AAGGAAGTGG TCAGTGAGAG TGAGACCGTG 
GCAGCAGAGC CTCCTCTGGA AAATGAGGCT GAAGAGAACT TCAGCCAGAG AACAGTGCCT 
CCTGAGACCT TGGATGAGCA ACTCTCTGAC TTAAATGATG GGCTGTTGGA TGGGGAGCCC 
CTCAGGACAG GAAGCGAGAG CGAGTCAAGC TCCAGGCCCA ATGGCGACAA CGAGTGCGAA 
GCGTGCGACA CCTCTTGCTA CAGCCCCTCT TGCTACAGCA CTTCCTGCTA CAGCACTTCC 
TGCTACAGCA CTTCTTGCTA CAGCACCTCG TGTTATGACA GTAACAATCG CTTCTCCAGC 
CATACTCGCT TCTCCTCCGT CGACAGCGCA AAGATTTCTG AGAGCACAGT CTTTTCCTCA 
CAGGATGATG ATGAGGAGGA GAACAGTGCT TTTGAGTCAG TGCCAGATTC AGTGCAGAGC 
CCTGAGCTGG ACAGGGAGCA AGTGGATGGC TCCTCCCAGT GGCCAGATGA ACTAGTAGCT 
CATGCTGGGA ATCCACAAAG AGCCACAGAG GGTCTAGACA CCCCAATAGC AGGTCCGAGC 
AGCAGAAGAG AAGGTGATTG TCCTTTACTC CATAATTCTC AGCCAGTCAG CCAGCTTCCT 
TCTCTGAGGC CTGACCATCA TCACTACCCA ACTATCGATG AGCCTCTTCC ACCAACTACA 
ACATCCTTGG GAAACTTTAC CTGGTGGAAT TGGGAAGCTC GGATAGACAG CCACGGGAGG 
GTATTCTACG TTGATCATGT CAACCGAACC ACCACGTGGC AGCGCCCCAC AGCCGCGGCA 
ACCCCGGATG GGATCCACAG GTCTGGCTCC ATCCAGCAAA TGGAGCAGCT GAACCGGCGA 
TACCAAAACA TCCAGCGAAC AATCGCAACA GAGCGGACTG ACGATGATGC TGTGGTAAAC 
AACAGGGTGG AGAGAGTTCC CACTGGAGGA GGAAGTGACT CTGAGGCAGA GCCTTCTCAG 
CCAAGCTCAG AGATTAGGAG GGAAGGTTCC CTTTCTCCTG TGAATTCTCA GAAAATCACC 
TTGTTGCTGC AGTCTCCAGC TGTGAAGTTC ATTACCAATC CTGAGTTCTT CACTGTGCTG 
CATGCAAACT ATAGTGCCTA TCGGGTCTTC ACTAACAGTA CCTGCTTGAA GCATATGATT 
CTGAAGATTC GTAGAGATGC TCGTAATTTT GAGCGGTATC AGCACAACAG AGACCTGGTA 
AATTTTATCA ACATGTTTGC TGATACCCGA CTGGAGCTTC CTCGTGGCTG GGAGATAAAA 
ACTGACCAGC AGGGCAAGTC TTTCTTCGTT GACCACAATA GCCGTGCTAC CACCTTCATA 
GATCCCAGGA TTCCGCTGCA GAATGGTCGT CTCCCTAATC ACCTGACTCA CAGGCAACAT 
CTTCAGCGAC TTCGTAGCTA CAGCGCTGGA GAGGCCTCTG AAGTCTCTCG AAACAGAGGA 
GTCACCCTGT TGGCCAGACC AGGCAATAGT CTTGTAACAG CTATTCGAAA CCAGCACCAT 
CATGAGGCAT TACCTCTGGC TTACAATGAC AAGATTGTTG CATTTTTACG CCAACCCAAC 
ATTTTTGAGA TGCTGCAGGA GCGTCAGCCC AGCTTGGCCA GAAACCATGC ACTCAGGGAG 
AAGATCCATT ACATTCGGAC AGAGGGTACA CATGGGCTTG AAAAATTGTC CTGTGATGCA 
GATTTAGTAA TTCTGCTGAG CCTTTTTGAA GAGGATATTA TGTCCTACAT ACCACTGCAG 
GCTGCCTTCC ATCCTGGTTA CAGTTTCTCT CCTCGCTGCT CACCCTGTTC ATCACCCCAA 
AATTCCCCAG GGCTGCAGAG AGCAAGTGCA AGAGCACCTT CTCCATACAG GAGGGACTTT 
GAAGCCAAGC TGCGCAATTT CTATCGAAAA CTTGAAGCCA AAGGGTATGG GCAAGGTCCA 
GGTAAAATTA AGTTAATTAT ACGGCGTGAC CACTTGCTGG AAGGCACCTT CAACCAGGTA 
ATGGCATATT CACGCAAGGA GCTCCAGCGG AACAAACTCT ACGTCACGTT TGTTGGCGAG 
GAAGGGTTGG ACTACAGTGG CCCCTCCCGA GAATTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAATCCTT ATTATGGGCT GTTTGAGTAT TCAGCCAATG ACACTTACAC TGTACAGATC 
AGCCCCATGT CTGCCTTTGT TGAAAATCAC CTGGAATGGT TCAGGTTCAG CGGTCGTATT 
CTTGGCCTTG CTCTCATTCA TCAGTACCTT CTTGATGCTT TCTTCACAAG GCCGTTCTAC 
AAAGCACTAC TAAGGCTACC ATGTGATTTA AGTGACTTAG AGTACCTGGA TGAAGAGTTC 
CATCAGAGCT TGCAGTGGAT GAAGGATAAC AACATCACAG ATATCTTGGA CTTAACCTTC 
ACAGTGAACG AGGAGGTATT TGGACAGGTG ACAGAGAGAG AGTTGAAATC TGGTGGGGCA 
AATACAGCAG TGACAGAAAA GAACAAAAAA GAGTACATCG AGAGGATGGT TAAGTGGCGA 
GTGGAACGAG GAGTGGTTCA GCAAACAGAG GCCCTGGTCC GTGGCTTCTA TGAAGTTGTA 
GACTCCAGGC TCGTCTCTGT TTTTGATGCC AGAGAACTGG AACTGGTTAT AGCTGGCACT 
GCAGAAATAG ATCTCAATGA CTGGCGGAAC AACACAGAAT ATCGTGGAGG TTACCATGAT 
GGACACATAG TAATACGGTG GTTCTGGGCA GCAGTGGAGA GGTTTAACAA CGAGCAGAGG 
CTCCGGCTGT TACAGTTCGT CACAGGAACA TCCAGCGTGC CATACGAGGG CTTTGCTGCT 
CTCCGAGGAA GCAACGGGCT CCGGCGCTTC TGTATAGAGA AATGGGGGAA AATAACCTCC 
CTCCCAAGGG CACATACATG TTTCAACCGT TTGGATCTTC CACCTTACCC CTCATATTCT 
ATGCTGTATG AAAAGCTGCT GACAGCTGTT GAAGAAACTA GCACCTTTGG ACTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017823169.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678658.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OLe49384
Clone ID Related Accession (Same CDS sequence)	XM_017823169.1
Accession Version	XM_017823169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGACTC AATCCAGGGA 
CAAGGAAATC GGAGACTGAT AAGTTTTTCA TTGTCAGATT TCCAGGCCTT TGGTCTGAAG 
AAAGGAATGT TCTTTAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGAAAACAT 
AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GATCTAAGAT CATGTGTAAT 
ACCGTCAACC CGATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCCCTGCC CACGGACGTC 
CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA ACGTTTTCTG 
GGAAAACTTT CTATGCCGGT TCAACGCCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT 
GTAAGCTACA CTCTGGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTT 
CGATTTGAAA TAACTTCTTC CATCCATCCA GATGATGAAG AGGTCTCCAT TAGTGCAGAT 
CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAATC CCACAGAGGA AAGCCTCGGT 
GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAGTGCAG CTCCAGAACA GCTAAATGGA 
TACAGTGTGA ACAGTGTCGA TAGCCCAGGG GCTGTCAAAC CACCTGGGGA AGTCAACCAG 
AACAGCTTAA ATGAAGAGCT AGCAGGAGAT GGGGAGGTTG ATGCGGTGCC AGTTCCTGAA 
CCCTTGGAAT CCATCCCAGG AGAAATGCTT TCTTCTTTGA GTGCTACGGA CCTCACAGAG 
CAGTCGACTC TGATGGCTTG CATGTCTCCT CCTGAGACTG AAGTTAGGGA AAATAACAAA 
GTCAATTCTG GTATTCAGGG AGATGGTGGA GTGACCAGCA TAGAAACATT AGATATTGAT 
GAGGTGAGTG CAGATGTGCG TGCTGGCAAG GACTTAGAGA AAAGTCAGGA AGAAGATGAA 
GGGGCTTCAG CCCAGGAGGA GGAAGACAAC AAGCTACAAC TGAGGACCAC AGCAAAGAGG 
AAAAACAGGC CGTGCTCCCT GCCTGTGTCT GAACTTGAGA CGGTCATAGC TTCAGCCTGC 
GGTGAGCCAG AGACCCCTCG CACACACTAC ATACGGATCC ACAACCTACT CCACAGCCTG 
CCCTCGGCAC AGATGAGCAG CGATGGGGAA GAAGAACAAG ATGGTGGCAA TGGCGGGGAG 
AACGATGAGG AATCTACAGT CAAGGAGGTG TTGGATAAGG AAGTGGTCAG TGAGAGTGAG 
ACCGTGGCAG CAGAGCCTCC TCTGGAAAAT GAGGCTGAAG AGAACTTCAG CCAGAGAACA 
GTGCCTCCTG AGACCTTGGA TGAGCAACTC TCTGACTTAA ATGATGGGCT GTTGGATGGG 
GAGCCCCTCA GGACAGGAAG CGAGAGCGAG TCAAGCTCCA GGCCCAATGG CGACAACGAG 
TGCGAAGCGT GCGACACCTC TTGCTACAGC CCCTCTTGCT ACAGCACTTC CTGCTACAGC 
ACTTCCTGCT ACAGCACTTC TTGCTACAGC ACCTCGTGTT ATGACAGTAA CAATCGCTTC 
TCCAGCCATA CTCGCTTCTC CTCCGTCGAC AGCGCAAAGA TTTCTGAGAG CACAGTCTTT 
TCCTCACAGG ATGATGATGA GGAGGAGAAC AGTGCTTTTG AGTCAGTGCC AGATTCAGTG 
CAGAGCCCTG AGCTGGACAG GGAGCAAGTG GATGGCTCCT CCCAGTGGCC AGATGAACTA 
GTAGCTCATG CTGGGAATCC ACAAAGAGCC ACAGAGGGTC TAGACACCCC AATAGCAGGT 
CCGAGCAGCA GAAGAGAAGA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTATTCTAC 
GTTGATCATG TCAACCGAAC CACCACGTGG CAGCGCCCCA CAGCCGCGGC AACCCCGGAT 
GGGATCCACA GGTCTGGCTC CATCCAGCAA ATGGAGCAGC TGAACCGGCG ATACCAAAAC 
ATCCAGCGAA CAATCGCAAC AGAGCGGACT GACGATGATG CTGTGGTAAA CAACAGGGTG 
GAGAGAGTTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCTCA GCCAAGCTCA 
GAGATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AGAAAATCAC CTTGTTGCTG 
CAGTCTCCAG CTGTGAAGTT CATTACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC 
TATAGTGCCT ATCGGGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT TCTGAAGATT 
CGTAGAGATG CTCGTAATTT TGAGCGGTAT CAGCACAACA GAGACCTGGT AAATTTTATC 
AACATGTTTG CTGATACCCG ACTGGAGCTT CCTCGTGGCT GGGAGATAAA AACTGACCAG 
CAGGGCAAGT CTTTCTTCGT TGACCACAAT AGCCGTGCTA CCACCTTCAT AGATCCCAGG 
ATTCCGCTGC AGAATGGTCG TCTCCCTAAT CACCTGACTC ACAGGCAACA TCTTCAGCGA 
CTTCGTAGCT ACAGCGCTGG AGAGGCCTCT GAAGTCTCTC GAAACAGAGG AGTCACCCTG 
TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCACCA TCATGAGGCA 
TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTTTAC GCCAACCCAA CATTTTTGAG 
ATGCTGCAGG AGCGTCAGCC CAGCTTGGCC AGAAACCATG CACTCAGGGA GAAGATCCAT 
TACATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAATTGT CCTGTGATGC AGATTTAGTA 
ATTCTGCTGA GCCTTTTTGA AGAGGATATT ATGTCCTACA TACCACTGCA GGCTGCCTTC 
CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGTT CATCACCCCA AAATTCCCCA 
GGGCTGCAGA GAGCAAGTGC AAGAGCACCT TCTCCATACA GGAGGGACTT TGAAGCCAAG 
CTGCGCAATT TCTATCGAAA ACTTGAAGCC AAAGGGTATG GGCAAGGTCC AGGTAAAATT 
AAGTTAATTA TACGGCGTGA CCACTTGCTG GAAGGCACCT TCAACCAGGT AATGGCATAT 
TCACGCAAGG AGCTCCAGCG GAACAAACTC TACGTCACGT TTGTTGGCGA GGAAGGGTTG 
GACTACAGTG GCCCCTCCCG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTCAATCCT 
TATTATGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT CAGCCCCATG 
TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GCGGTCGTAT TCTTGGCCTT 
GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA 
CTAAGGCTAC CATGTGATTT AAGTGACTTA GAGTACCTGG ATGAAGAGTT CCATCAGAGC 
TTGCAGTGGA TGAAGGATAA CAACATCACA GATATCTTGG ACTTAACCTT CACAGTGAAC 
GAGGAGGTAT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGTGGGGC AAATACAGCA 
GTGACAGAAA AGAACAAAAA AGAGTACATC GAGAGGATGG TTAAGTGGCG AGTGGAACGA 
GGAGTGGTTC AGCAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCCAGG 
CTCGTCTCTG TTTTTGATGC CAGAGAACTG GAACTGGTTA TAGCTGGCAC TGCAGAAATA 
GATCTCAATG ACTGGCGGAA CAACACAGAA TATCGTGGAG GTTACCATGA TGGACACATA 
GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ACGAGCAGAG GCTCCGGCTG 
TTACAGTTCG TCACAGGAAC ATCCAGCGTG CCATACGAGG GCTTTGCTGC TCTCCGAGGA 
AGCAACGGGC TCCGGCGCTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTCCCAAGG 
GCACATACAT GTTTCAACCG TTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT 
GAAAAGCTGC TGACAGCTGT TGAAGAAACT AGCACCTTTG GACTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678658.1
CDS	165..4895
Translation	MSCAQAGIVQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPQIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823169.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823169.1

ORF Insert Sequence:

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGACTC AATCCAGGGA 
CAAGGAAATC GGAGACTGAT AAGTTTTTCA TTGTCAGATT TCCAGGCCTT TGGTCTGAAG 
AAAGGAATGT TCTTTAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGAAAACAT 
AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GATCTAAGAT CATGTGTAAT 
ACCGTCAACC CGATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCCCTGCC CACGGACGTC 
CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA ACGTTTTCTG 
GGAAAACTTT CTATGCCGGT TCAACGCCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT 
GTAAGCTACA CTCTGGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTT 
CGATTTGAAA TAACTTCTTC CATCCATCCA GATGATGAAG AGGTCTCCAT TAGTGCAGAT 
CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAATC CCACAGAGGA AAGCCTCGGT 
GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAGTGCAG CTCCAGAACA GCTAAATGGA 
TACAGTGTGA ACAGTGTCGA TAGCCCAGGG GCTGTCAAAC CACCTGGGGA AGTCAACCAG 
AACAGCTTAA ATGAAGAGCT AGCAGGAGAT GGGGAGGTTG ATGCGGTGCC AGTTCCTGAA 
CCCTTGGAAT CCATCCCAGG AGAAATGCTT TCTTCTTTGA GTGCTACGGA CCTCACAGAG 
CAGTCGACTC TGATGGCTTG CATGTCTCCT CCTGAGACTG AAGTTAGGGA AAATAACAAA 
GTCAATTCTG GTATTCAGGG AGATGGTGGA GTGACCAGCA TAGAAACATT AGATATTGAT 
GAGGTGAGTG CAGATGTGCG TGCTGGCAAG GACTTAGAGA AAAGTCAGGA AGAAGATGAA 
GGGGCTTCAG CCCAGGAGGA GGAAGACAAC AAGCTACAAC TGAGGACCAC AGCAAAGAGG 
AAAAACAGGC CGTGCTCCCT GCCTGTGTCT GAACTTGAGA CGGTCATAGC TTCAGCCTGC 
GGTGAGCCAG AGACCCCTCG CACACACTAC ATACGGATCC ACAACCTACT CCACAGCCTG 
CCCTCGGCAC AGATGAGCAG CGATGGGGAA GAAGAACAAG ATGGTGGCAA TGGCGGGGAG 
AACGATGAGG AATCTACAGT CAAGGAGGTG TTGGATAAGG AAGTGGTCAG TGAGAGTGAG 
ACCGTGGCAG CAGAGCCTCC TCTGGAAAAT GAGGCTGAAG AGAACTTCAG CCAGAGAACA 
GTGCCTCCTG AGACCTTGGA TGAGCAACTC TCTGACTTAA ATGATGGGCT GTTGGATGGG 
GAGCCCCTCA GGACAGGAAG CGAGAGCGAG TCAAGCTCCA GGCCCAATGG CGACAACGAG 
TGCGAAGCGT GCGACACCTC TTGCTACAGC CCCTCTTGCT ACAGCACTTC CTGCTACAGC 
ACTTCCTGCT ACAGCACTTC TTGCTACAGC ACCTCGTGTT ATGACAGTAA CAATCGCTTC 
TCCAGCCATA CTCGCTTCTC CTCCGTCGAC AGCGCAAAGA TTTCTGAGAG CACAGTCTTT 
TCCTCACAGG ATGATGATGA GGAGGAGAAC AGTGCTTTTG AGTCAGTGCC AGATTCAGTG 
CAGAGCCCTG AGCTGGACAG GGAGCAAGTG GATGGCTCCT CCCAGTGGCC AGATGAACTA 
GTAGCTCATG CTGGGAATCC ACAAAGAGCC ACAGAGGGTC TAGACACCCC AATAGCAGGT 
CCGAGCAGCA GAAGAGAAGA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTATTCTAC 
GTTGATCATG TCAACCGAAC CACCACGTGG CAGCGCCCCA CAGCCGCGGC AACCCCGGAT 
GGGATCCACA GGTCTGGCTC CATCCAGCAA ATGGAGCAGC TGAACCGGCG ATACCAAAAC 
ATCCAGCGAA CAATCGCAAC AGAGCGGACT GACGATGATG CTGTGGTAAA CAACAGGGTG 
GAGAGAGTTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCTCA GCCAAGCTCA 
GAGATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AGAAAATCAC CTTGTTGCTG 
CAGTCTCCAG CTGTGAAGTT CATTACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC 
TATAGTGCCT ATCGGGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT TCTGAAGATT 
CGTAGAGATG CTCGTAATTT TGAGCGGTAT CAGCACAACA GAGACCTGGT AAATTTTATC 
AACATGTTTG CTGATACCCG ACTGGAGCTT CCTCGTGGCT GGGAGATAAA AACTGACCAG 
CAGGGCAAGT CTTTCTTCGT TGACCACAAT AGCCGTGCTA CCACCTTCAT AGATCCCAGG 
ATTCCGCTGC AGAATGGTCG TCTCCCTAAT CACCTGACTC ACAGGCAACA TCTTCAGCGA 
CTTCGTAGCT ACAGCGCTGG AGAGGCCTCT GAAGTCTCTC GAAACAGAGG AGTCACCCTG 
TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCACCA TCATGAGGCA 
TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTTTAC GCCAACCCAA CATTTTTGAG 
ATGCTGCAGG AGCGTCAGCC CAGCTTGGCC AGAAACCATG CACTCAGGGA GAAGATCCAT 
TACATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAATTGT CCTGTGATGC AGATTTAGTA 
ATTCTGCTGA GCCTTTTTGA AGAGGATATT ATGTCCTACA TACCACTGCA GGCTGCCTTC 
CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGTT CATCACCCCA AAATTCCCCA 
GGGCTGCAGA GAGCAAGTGC AAGAGCACCT TCTCCATACA GGAGGGACTT TGAAGCCAAG 
CTGCGCAATT TCTATCGAAA ACTTGAAGCC AAAGGGTATG GGCAAGGTCC AGGTAAAATT 
AAGTTAATTA TACGGCGTGA CCACTTGCTG GAAGGCACCT TCAACCAGGT AATGGCATAT 
TCACGCAAGG AGCTCCAGCG GAACAAACTC TACGTCACGT TTGTTGGCGA GGAAGGGTTG 
GACTACAGTG GCCCCTCCCG AGAATTCTTC TTCCTTCTGT CTCAGGAGCT CTTCAATCCT 
TATTATGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT CAGCCCCATG 
TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GCGGTCGTAT TCTTGGCCTT 
GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA 
CTAAGGCTAC CATGTGATTT AAGTGACTTA GAGTACCTGG ATGAAGAGTT CCATCAGAGC 
TTGCAGTGGA TGAAGGATAA CAACATCACA GATATCTTGG ACTTAACCTT CACAGTGAAC 
GAGGAGGTAT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGTGGGGC AAATACAGCA 
GTGACAGAAA AGAACAAAAA AGAGTACATC GAGAGGATGG TTAAGTGGCG AGTGGAACGA 
GGAGTGGTTC AGCAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCCAGG 
CTCGTCTCTG TTTTTGATGC CAGAGAACTG GAACTGGTTA TAGCTGGCAC TGCAGAAATA 
GATCTCAATG ACTGGCGGAA CAACACAGAA TATCGTGGAG GTTACCATGA TGGACACATA 
GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ACGAGCAGAG GCTCCGGCTG 
TTACAGTTCG TCACAGGAAC ATCCAGCGTG CCATACGAGG GCTTTGCTGC TCTCCGAGGA 
AGCAACGGGC TCCGGCGCTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTCCCAAGG 
GCACATACAT GTTTCAACCG TTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT 
GAAAAGCTGC TGACAGCTGT TGAAGAAACT AGCACCTTTG GACTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017823179.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678668.1 Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OLe49385
Clone ID Related Accession (Same CDS sequence)	XM_017823179.1
Accession Version	XM_017823179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	E3 ubiquitin-protein ligase HECW1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGATTG GGAAGCTCGG ATAGACAGCC ACGGGAGGGT ATTCTACGTT 
GATCATGTCA ACCGAACCAC CACGTGGCAG CGCCCCACAG CCGCGGCAAC CCCGGATGGG 
ATCCACAGGT CTGGCTCCAT CCAGCAAATG GAGCAGCTGA ACCGGCGATA CCAAAACATC 
CAGCGAACAA TCGCAACAGA GCGGACTGAC GATGATGCTG TGGTAAACAA CAGGGTGGAG 
AGAGTTCCCA CTGGAGGAGG AAGTGACTCT GAGGCAGAGC CTTCTCAGCC AAGCTCAGAG 
ATTAGGAGGG AAGGTTCCCT TTCTCCTGTG AATTCTCAGA AAATCACCTT GTTGCTGCAG 
TCTCCAGCTG TGAAGTTCAT TACCAATCCT GAGTTCTTCA CTGTGCTGCA TGCAAACTAT 
AGTGCCTATC GGGTCTTCAC TAACAGTACC TGCTTGAAGC ATATGATTCT GAAGATTCGT 
AGAGATGCTC GTAATTTTGA GCGGTATCAG CACAACAGAG ACCTGGTAAA TTTTATCAAC 
ATGTTTGCTG ATACCCGACT GGAGCTTCCT CGTGGCTGGG AGATAAAAAC TGACCAGCAG 
GGCAAGTCTT TCTTCGTTGA CCACAATAGC CGTGCTACCA CCTTCATAGA TCCCAGGATT 
CCGCTGCAGA ATGGTCGTCT CCCTAATCAC CTGACTCACA GGCAACATCT TCAGCGACTT 
CGTAGCTACA GCGCTGGAGA GGCCTCTGAA GTCTCTCGAA ACAGAGGAGT CACCCTGTTG 
GCCAGACCAG GCAATAGTCT TGTAACAGCT ATTCGAAACC AGCACCATCA TGAGGCATTA 
CCTCTGGCTT ACAATGACAA GATTGTTGCA TTTTTACGCC AACCCAACAT TTTTGAGATG 
CTGCAGGAGC GTCAGCCCAG CTTGGCCAGA AACCATGCAC TCAGGGAGAA GATCCATTAC 
ATTCGGACAG AGGGTACACA TGGGCTTGAA AAATTGTCCT GTGATGCAGA TTTAGTAATT 
CTGCTGAGCC TTTTTGAAGA GGATATTATG TCCTACATAC CACTGCAGGC TGCCTTCCAT 
CCTGGTTACA GTTTCTCTCC TCGCTGCTCA CCCTGTTCAT CACCCCAAAA TTCCCCAGGG 
CTGCAGAGAG CAAGTGCAAG AGCACCTTCT CCATACAGGA GGGACTTTGA AGCCAAGCTG 
CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGGTATGGGC AAGGTCCAGG TAAAATTAAG 
TTAATTATAC GGCGTGACCA CTTGCTGGAA GGCACCTTCA ACCAGGTAAT GGCATATTCA 
CGCAAGGAGC TCCAGCGGAA CAAACTCTAC GTCACGTTTG TTGGCGAGGA AGGGTTGGAC 
TACAGTGGCC CCTCCCGAGA ATTCTTCTTC CTTCTGTCTC AGGAGCTCTT CAATCCTTAT 
TATGGGCTGT TTGAGTATTC AGCCAATGAC ACTTACACTG TACAGATCAG CCCCATGTCT 
GCCTTTGTTG AAAATCACCT GGAATGGTTC AGGTTCAGCG GTCGTATTCT TGGCCTTGCT 
CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTACAA AGCACTACTA 
AGGCTACCAT GTGATTTAAG TGACTTAGAG TACCTGGATG AAGAGTTCCA TCAGAGCTTG 
CAGTGGATGA AGGATAACAA CATCACAGAT ATCTTGGACT TAACCTTCAC AGTGAACGAG 
GAGGTATTTG GACAGGTGAC AGAGAGAGAG TTGAAATCTG GTGGGGCAAA TACAGCAGTG 
ACAGAAAAGA ACAAAAAAGA GTACATCGAG AGGATGGTTA AGTGGCGAGT GGAACGAGGA 
GTGGTTCAGC AAACAGAGGC CCTGGTCCGT GGCTTCTATG AAGTTGTAGA CTCCAGGCTC 
GTCTCTGTTT TTGATGCCAG AGAACTGGAA CTGGTTATAG CTGGCACTGC AGAAATAGAT 
CTCAATGACT GGCGGAACAA CACAGAATAT CGTGGAGGTT ACCATGATGG ACACATAGTA 
ATACGGTGGT TCTGGGCAGC AGTGGAGAGG TTTAACAACG AGCAGAGGCT CCGGCTGTTA 
CAGTTCGTCA CAGGAACATC CAGCGTGCCA TACGAGGGCT TTGCTGCTCT CCGAGGAAGC 
AACGGGCTCC GGCGCTTCTG TATAGAGAAA TGGGGGAAAA TAACCTCCCT CCCAAGGGCA 
CATACATGTT TCAACCGTTT GGATCTTCCA CCTTACCCCT CATATTCTAT GCTGTATGAA 
AAGCTGCTGA CAGCTGTTGA AGAAACTAGC ACCTTTGGAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678668.1
CDS	165..4892
Translation	MSCAQAGIVQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFHNMDIRNNSHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNNAAPIFKSITNEDSIQGQGNRRLISFSLSDFQAFGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIMCNTVNPIWQGEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPEPADLQESPVNNPTEESLGEPPCINTVTSESAAPEQLNGYSVNSVDSPGAVKPPGEVNQNSLNEELAGDGEVDAVPVPEPLESIPGEMLSSLSATDLTEQSTLMACMSPPETEVRENNKVNSGIQGDGGVTSIETLDIDEVSADVRAGKDLEKSQEEDEGASAQEEEDNKLQLRTTAKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEQDGGNGGENDEESTVKEVLDKEVVSESETVAAEPPLENEAEENFSQRTVPPETLDEQLSDLNDGLLDGEPLRTGSESESSSRPNGDNECEACDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDDEEENSAFESVPDSVQSPELDREQVDGSSQWPDELVAHAGNPQRATEGLDTPIAGPSSRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIHRSGSIQQMEQLNRRYQNIQRTIATERTDDDAVVNNRVERVPTGGGSDSEAEPSQPSSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGVTLLARPGNSLVTAIRNQHHHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTAVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017823179.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823179.1

ORF Insert Sequence:

ATGAGCTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTG 
GCAGCCATGG CGTCTCCATC TAGAAACTCA CAAAATCGAC GCCGGTGCAA AGAGCCTCTC 
AGATACAGCT ATAACCCTGA CCAGTTCCAT AACATGGACA TCAGGAACAA TTCCCATGAG 
ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACAGACCTTG TCACTTCAGA CAGCCGGTCC 
ACCCTGATGG TCAGCAGCTC CTACTACTCC ATTGGGCATT CGCAGGACCT AGTGATATAC 
TGGGATATAA AAGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAG 
GTCTTGTCTG AAAACTTTCT GGACTACAAG AACCGCGGTG TCAATGGCTC TCACCGTGGA 
CAGATTATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AGCCTGAAAC AAAGATCTGC 
TTCAAGTACT ACCATGGTGT GAGTGGGGCC CTACGAGCCA CAACTCCAAG TGTCACAGTG 
AAAAACAATG CAGCTCCTAT TTTTAAAAGC ATCACCAATG AAGACTCAAT CCAGGGACAA 
GGAAATCGGA GACTGATAAG TTTTTCATTG TCAGATTTCC AGGCCTTTGG TCTGAAGAAA 
GGAATGTTCT TTAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG AAAACATAGC 
ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGAT CTAAGATCAT GTGTAATACC 
GTCAACCCGA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CCCTGCCCAC GGACGTCCTG 
GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAACG TTTTCTGGGA 
AAACTTTCTA TGCCGGTTCA ACGCCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA 
AGCTACACTC TGGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTTCGA 
TTTGAAATAA CTTCTTCCAT CCATCCAGAT GATGAAGAGG TCTCCATTAG TGCAGATCCT 
GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAATCCCA CAGAGGAAAG CCTCGGTGAG 
CCTCCATGCA TCAACACAGT GACCTCAGAA AGTGCAGCTC CAGAACAGCT AAATGGATAC 
AGTGTGAACA GTGTCGATAG CCCAGGGGCT GTCAAACCAC CTGGGGAAGT CAACCAGAAC 
AGCTTAAATG AAGAGCTAGC AGGAGATGGG GAGGTTGATG CGGTGCCAGT TCCTGAACCC 
TTGGAATCCA TCCCAGGAGA AATGCTTTCT TCTTTGAGTG CTACGGACCT CACAGAGCAG 
TCGACTCTGA TGGCTTGCAT GTCTCCTCCT GAGACTGAAG TTAGGGAAAA TAACAAAGTC 
AATTCTGGTA TTCAGGGAGA TGGTGGAGTG ACCAGCATAG AAACATTAGA TATTGATGAG 
GTGAGTGCAG ATGTGCGTGC TGGCAAGGAC TTAGAGAAAA GTCAGGAAGA AGATGAAGGG 
GCTTCAGCCC AGGAGGAGGA AGACAACAAG CTACAACTGA GGACCACAGC AAAGAGGAAA 
AACAGGCCGT GCTCCCTGCC TGTGTCTGAA CTTGAGACGG TCATAGCTTC AGCCTGCGGT 
GAGCCAGAGA CCCCTCGCAC ACACTACATA CGGATCCACA ACCTACTCCA CAGCCTGCCC 
TCGGCACAGA TGAGCAGCGA TGGGGAAGAA GAACAAGATG GTGGCAATGG CGGGGAGAAC 
GATGAGGAAT CTACAGTCAA GGAGGTGTTG GATAAGGAAG TGGTCAGTGA GAGTGAGACC 
GTGGCAGCAG AGCCTCCTCT GGAAAATGAG GCTGAAGAGA ACTTCAGCCA GAGAACAGTG 
CCTCCTGAGA CCTTGGATGA GCAACTCTCT GACTTAAATG ATGGGCTGTT GGATGGGGAG 
CCCCTCAGGA CAGGAAGCGA GAGCGAGTCA AGCTCCAGGC CCAATGGCGA CAACGAGTGC 
GAAGCGTGCG ACACCTCTTG CTACAGCCCC TCTTGCTACA GCACTTCCTG CTACAGCACT 
TCCTGCTACA GCACTTCTTG CTACAGCACC TCGTGTTATG ACAGTAACAA TCGCTTCTCC 
AGCCATACTC GCTTCTCCTC CGTCGACAGC GCAAAGATTT CTGAGAGCAC AGTCTTTTCC 
TCACAGGATG ATGATGAGGA GGAGAACAGT GCTTTTGAGT CAGTGCCAGA TTCAGTGCAG 
AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA 
GCTCATGCTG GGAATCCACA AAGAGCCACA GAGGGTCTAG ACACCCCAAT AGCAGGTCCG 
AGCAGCAGAA GAGAAGATTG GGAAGCTCGG ATAGACAGCC ACGGGAGGGT ATTCTACGTT 
GATCATGTCA ACCGAACCAC CACGTGGCAG CGCCCCACAG CCGCGGCAAC CCCGGATGGG 
ATCCACAGGT CTGGCTCCAT CCAGCAAATG GAGCAGCTGA ACCGGCGATA CCAAAACATC 
CAGCGAACAA TCGCAACAGA GCGGACTGAC GATGATGCTG TGGTAAACAA CAGGGTGGAG 
AGAGTTCCCA CTGGAGGAGG AAGTGACTCT GAGGCAGAGC CTTCTCAGCC AAGCTCAGAG 
ATTAGGAGGG AAGGTTCCCT TTCTCCTGTG AATTCTCAGA AAATCACCTT GTTGCTGCAG 
TCTCCAGCTG TGAAGTTCAT TACCAATCCT GAGTTCTTCA CTGTGCTGCA TGCAAACTAT 
AGTGCCTATC GGGTCTTCAC TAACAGTACC TGCTTGAAGC ATATGATTCT GAAGATTCGT 
AGAGATGCTC GTAATTTTGA GCGGTATCAG CACAACAGAG ACCTGGTAAA TTTTATCAAC 
ATGTTTGCTG ATACCCGACT GGAGCTTCCT CGTGGCTGGG AGATAAAAAC TGACCAGCAG 
GGCAAGTCTT TCTTCGTTGA CCACAATAGC CGTGCTACCA CCTTCATAGA TCCCAGGATT 
CCGCTGCAGA ATGGTCGTCT CCCTAATCAC CTGACTCACA GGCAACATCT TCAGCGACTT 
CGTAGCTACA GCGCTGGAGA GGCCTCTGAA GTCTCTCGAA ACAGAGGAGT CACCCTGTTG 
GCCAGACCAG GCAATAGTCT TGTAACAGCT ATTCGAAACC AGCACCATCA TGAGGCATTA 
CCTCTGGCTT ACAATGACAA GATTGTTGCA TTTTTACGCC AACCCAACAT TTTTGAGATG 
CTGCAGGAGC GTCAGCCCAG CTTGGCCAGA AACCATGCAC TCAGGGAGAA GATCCATTAC 
ATTCGGACAG AGGGTACACA TGGGCTTGAA AAATTGTCCT GTGATGCAGA TTTAGTAATT 
CTGCTGAGCC TTTTTGAAGA GGATATTATG TCCTACATAC CACTGCAGGC TGCCTTCCAT 
CCTGGTTACA GTTTCTCTCC TCGCTGCTCA CCCTGTTCAT CACCCCAAAA TTCCCCAGGG 
CTGCAGAGAG CAAGTGCAAG AGCACCTTCT CCATACAGGA GGGACTTTGA AGCCAAGCTG 
CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGGTATGGGC AAGGTCCAGG TAAAATTAAG 
TTAATTATAC GGCGTGACCA CTTGCTGGAA GGCACCTTCA ACCAGGTAAT GGCATATTCA 
CGCAAGGAGC TCCAGCGGAA CAAACTCTAC GTCACGTTTG TTGGCGAGGA AGGGTTGGAC 
TACAGTGGCC CCTCCCGAGA ATTCTTCTTC CTTCTGTCTC AGGAGCTCTT CAATCCTTAT 
TATGGGCTGT TTGAGTATTC AGCCAATGAC ACTTACACTG TACAGATCAG CCCCATGTCT 
GCCTTTGTTG AAAATCACCT GGAATGGTTC AGGTTCAGCG GTCGTATTCT TGGCCTTGCT 
CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTACAA AGCACTACTA 
AGGCTACCAT GTGATTTAAG TGACTTAGAG TACCTGGATG AAGAGTTCCA TCAGAGCTTG 
CAGTGGATGA AGGATAACAA CATCACAGAT ATCTTGGACT TAACCTTCAC AGTGAACGAG 
GAGGTATTTG GACAGGTGAC AGAGAGAGAG TTGAAATCTG GTGGGGCAAA TACAGCAGTG 
ACAGAAAAGA ACAAAAAAGA GTACATCGAG AGGATGGTTA AGTGGCGAGT GGAACGAGGA 
GTGGTTCAGC AAACAGAGGC CCTGGTCCGT GGCTTCTATG AAGTTGTAGA CTCCAGGCTC 
GTCTCTGTTT TTGATGCCAG AGAACTGGAA CTGGTTATAG CTGGCACTGC AGAAATAGAT 
CTCAATGACT GGCGGAACAA CACAGAATAT CGTGGAGGTT ACCATGATGG ACACATAGTA 
ATACGGTGGT TCTGGGCAGC AGTGGAGAGG TTTAACAACG AGCAGAGGCT CCGGCTGTTA 
CAGTTCGTCA CAGGAACATC CAGCGTGCCA TACGAGGGCT TTGCTGCTCT CCGAGGAAGC 
AACGGGCTCC GGCGCTTCTG TATAGAGAAA TGGGGGAAAA TAACCTCCCT CCCAAGGGCA 
CATACATGTT TCAACCGTTT GGATCTTCCA CCTTACCCCT CATATTCTAT GCTGTATGAA 
AAGCTGCTGA CAGCTGTTGA AGAAACTAGC ACCTTTGGAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

HECW1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム