bmp2k cDNA ORF clone, Lates calcarifer(barramundi perch)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmp2k
Entrez Gene ID	108881612
Full Name	BMP2 inducible kinase
Gene Type	protein-coding
Organism	Lates calcarifer(barramundi perch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018673660.1	XP_018529176.1	BMP-2-inducible protein kinase isoform X2
XM_018673659.1	XP_018529175.1	BMP-2-inducible protein kinase isoform X1

Pathways from BioSystems
Gene Ontology

The following bmp2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa49954	XM_018673659.1 Latest version!	Lates calcarifer BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OLa49955	XM_018673660.1 Latest version!	Lates calcarifer BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp2k ( XM_018673659.1 ) cDNA ORF clone, Lates calcarifer(barramundi perch) -> XP_018529175.1 Lates calcarifer BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa49954
Clone ID Related Accession (Same CDS sequence)	XM_018673659.1
Accession Version	XM_018673659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-13
Organism	Lates calcarifer(barramundi perch)
Product	BMP-2-inducible protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017363863.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lates calcarifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGCGTCCGGG 
TCCGGGTCCA GCTCCGGAGT CAGCAGCTAC TTCGGGAAAG TGTTCGCCGT CGGCCGGTAT 
CAAGTCACCG TGGAGGAGCT TATTGCAGAA GGAGGCTTCT CGGTGGTGTT TCTGGCTCGT 
ACGCACAGCG GCGTCCGCTG CGCTCTCAAG AGGATGTATG TCAACAACGT CCCGGACCTG 
AACGTCTACA AGAGGGAGAT CACTATCATG AAGGAGCTGT CGGGCCACAA AAACATCGTC 
GGCTACCTGG ACTCCACGAT CAGTCCCGTG TCGGACAGCG TCTGGGAGGT CCTGATCCTC 
ATGGAGTACT GTAAAGCGGG TCAGGTGGTG AAGCAGATGA ACCAGCGGTT GAATGTGGGC 
TTCAGCGAGG CCGAGGTCCT TCACATCTTC TGTGACACCT GTGAGGCCGT GGCTCGCCTG 
CACCAATGCA AGACGCCCGT CATCCACAGA GACCTAAAGG TTGAAAACAT CCTGCTGAAC 
GACCAGGGAA ATTATGTGCT GTGTGACTTT GGAAGTGCCA CTCACAAGGT TTTGCTGCCA 
CATAAAGACG GAGTTACTGC TGTGGAGGAC GAGATTAAGA AGTACACGAC CCTGTCGTAT 
CGGGCGCCTG AGATGATCAA CTTGTACGCA GGGAAAGCCA TCACCACTAA AGCTGATATA 
TGGGCACTTG GATGCTTGTT GTACAAGCTG TGTTTCTTCG CACTTCCATT TGGGGAGAGT 
CAAGTTGCCA TATGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAATT CTCCTTCAAG 
TTGCACTGCT TAATCAGATA TATGCTCGAA CCAGACCAGG AGAAGAGACC AGACATTTAC 
CAAGTGTCCT ACTTTGCCTT CAAGTTGGCT GGAAAAGACT GTCCAGTGCC AAATCTCTTT 
AACTCTCCTA TCCCTACGTC GCTCCCAGAG CCTCTAACGG CCAGCGAGGT CGCTGCTAAG 
AAAAGTATGA CAAAAGCCAG GATAACAGAC TCTGTCGGCC CGACGGAAAC ATCGATAGCT 
CCCAGACAGA GGCCGAAGGC TGCCAACAGT AACGTCCTGC CCCTCGCCAA CACTGTCACC 
CCTGTCAAGA TGACCATGCC CTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACCCCT 
GGCTCCGGGC AGCCGTCCAT GCAGCCCCAG CCTCAGCCCA GCAGTCAGCA GCACCGAGTC 
CTGCAGCAGC TACAGCCTGG CGACCTGCGT CTACAACAGC TACAACAACA TCACCACCAC 
CAACAACAAC AACAACAACA AGCAGTGCAG CAGCAACACG CTAACGCACA GCAACTCCAA 
TACCTCCAGT ACCAACAGGC GGTGCAGCAG TCCCTCCAGC TTCAGCAGCA GCAGGCCCTG 
CTCCAGCAGC AGCAGCAGAT GATGATGCAG CCGATGTACC AGCAGCAGGT TGCTCAGGCC 
CAGGCCCAGG CTCAGGCTCA GGCTCAGGCT CAGGCTCAGT ACGCAGCCAT GCTGCACCAG 
TACCAACAGG CTTTTGTCCA GCAACAGCAG CAACAACAAC AGCATCATCA ACATCATCAA 
CAACAACAAC AACATCAGCA GCAGCAGCAT CATCCCGCTC AGCAGCCTCT CCCCTATATG 
TCCTCCCCTC TTGAGTTCCA GATCCCACTG GGGACCTACA GCGCCACTAC ACCCACCGCA 
GGAACTGGAG CTGGACCTGG TCAGATGGGG GGACTGTCAC CTGTGGATCC CTCATTCACT 
AACCCCAGTA GAAACCCTCT CCTCGCCTCA GATGGGATCA CCCCGCCCTC TCAGAGCTGC 
AACAGTACAG TCAGTAACCC CCCCGACATG TCGGGCTGGA ACCCTTTCGG AGAGGACAAC 
TTCTCCAAGT TGACCGAGGA GGAGCTGATC GACCGGGAGT TCGACATGCT CAGAGCAAAC 
AAACCGGTGG AGAGAACAGC CAGTGTGGAA ACGGACCGAC CGCAGCCGAC CGCCGCCAAG 
CCTCTCCCTC CAGAGGACCT GTTCGGCTCA GTCCCGTTCG TGGCCAGCGC AGGTACCAGT 
GTGATAAAGA TTGAGCAGAC CCCTGAGGAA GTCGCCGTTC CCGCCCCCGT CTCGACCACC 
ACAGACAATC TGCAGCCGAC AGCAGCCAAG GAGCACAAAC CAGGCAGGAA GAGTAACTGT 
AGACCAGGTG AAGAGTCCGA CAGCGACTTC GAGTCTGATC CTCCTTCGCC GAAAAGCAGC 
GAAGATGAAG AGCCGGAGGA GGACGAGGCG CTGAACAGCG AGCACGGTGA AGACACAGAG 
CCGGAGAATT TAGGACAGAG GCCTCTGCTC ATGGACTCAG AGGAAGAGGG CGAGGAGGAC 
GATGACAAGC ACAGCTCTGA CTCGGACTAC GACCCCAGCA GAGTCAAGAC TCGCTCCAAG 
AAACCTGTGG CGGGTAAAGC AGCTTCTGCA GCAACCGGGA GGAGTCCTCG AGGAGATTCT 
GCCATGACCC TGATCACCCC TCCTGAGTCG CCCAGCGGAG CAAGAGCTGC TCCGGTCACG 
GATGATGTGG ATGTTTTCGG TGCCGTTCCC TTCCTCGGAG GAGCCTCACC TGTCGGACCT 
TCAGAGAACA CGGACATCTT TGCCAAAGCG CCTTTCAGGC AAGTCAGCCA CGAGCAGCAA 
GCTCCGGATG AGTTTGATGT TTTTACCAAA GCGCCATTCA ACCGGAACCT CTCCAAGTCT 
GGCAAGAGTG GCAGTGATGT TCCCATGGGC CAAACGCCGC CCATTTCCCC CGAGGGCATT 
GACATTTTCG GCTTCTCTCC CTTCCAGCCG GGCCCCACCG CGCCACCTCC CATCACCTCC 
AGAAGTGCGG ATGATATTTT TCGGCCATCT TACGAGGAGT CGCCGAGTCC TCAGCAGCAG 
AGGCTGAAGC AGCGCAGCCT CCAGAAGCTG TCGTCACGCC AGAGAAAAAC CAAGCAAGAG 
GCCACGGCGG GCGGCAGCAA CGGTAAACGG CATCACGGTA CACCTACTGG AGGGCGCAAG 
ACCAACAAGC CGACTTACCG CACACCAGAG CGAGTCCGCA GGCATAAGAA GGTCGGCCGG 
AGAGACTCGC AGAGCAGCAA CGAGTTCCTC AGCACCTCTG ACTCTAAAGA GAACATCAGT 
GTGGACATAA CCATGTCTGA AGGGAAAGAC AAAGGAGCTT CTCTGCCGGT AGACGAGGCC 
CTTTTAGACC CGTTTGGGGC CAAACCCTTC CATCCTCAGG ACGGATTACG GCACAACCAG 
TATCAAGGCC TCGGTGACAG TAAGAGCGAC ATGGGCACGG CCAACAGCAA GTCCAGGACG 
GGTTCTCTTC ACGGAGCTCT CGGAGAAAGC AAGATCATGG ATGATTTCGG GGCGGTGCCC 
TTCACAGAAC TGGTCATCCA CAGTGGACCT CAGCAGCAGC AGCAGCAGCA GCAGGCGCCC 
TCGCAGCCGG TGGAGCTTGA CCCGTTCGGA GCTGCACCTT TTCCCTCAAA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018529175.1
CDS	897..4433
Translation	MKKFSRMPKSESGGLGGASGSGSSSGVSSYFGKVFAVGRYQVTVEELIAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNVYKREITIMKELSGHKNIVGYLDSTISPVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPVIHRDLKVENILLNDQGNYVLCDFGSATHKVLLPHKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKAITTKADIWALGCLLYKLCFFALPFGESQVAICDGTFIVPDNSKFSFKLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGKDCPVPNLFNSPIPTSLPEPLTASEVAAKKSMTKARITDSVGPTETSIAPRQRPKAANSNVLPLANTVTPVKMTMPSAPVSNGQKAPTPGSGQPSMQPQPQPSSQQHRVLQQLQPGDLRLQQLQQHHHHQQQQQQQAVQQQHANAQQLQYLQYQQAVQQSLQLQQQQALLQQQQQMMMQPMYQQQVAQAQAQAQAQAQAQAQYAAMLHQYQQAFVQQQQQQQQHHQHHQQQQQHQQQQHHPAQQPLPYMSSPLEFQIPLGTYSATTPTAGTGAGPGQMGGLSPVDPSFTNPSRNPLLASDGITPPSQSCNSTVSNPPDMSGWNPFGEDNFSKLTEEELIDREFDMLRANKPVERTASVETDRPQPTAAKPLPPEDLFGSVPFVASAGTSVIKIEQTPEEVAVPAPVSTTTDNLQPTAAKEHKPGRKSNCRPGEESDSDFESDPPSPKSSEDEEPEEDEALNSEHGEDTEPENLGQRPLLMDSEEEGEEDDDKHSSDSDYDPSRVKTRSKKPVAGKAASAATGRSPRGDSAMTLITPPESPSGARAAPVTDDVDVFGAVPFLGGASPVGPSENTDIFAKAPFRQVSHEQQAPDEFDVFTKAPFNRNLSKSGKSGSDVPMGQTPPISPEGIDIFGFSPFQPGPTAPPPITSRSADDIFRPSYEESPSPQQQRLKQRSLQKLSSRQRKTKQEATAGGSNGKRHHGTPTGGRKTNKPTYRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISVDITMSEGKDKGASLPVDEALLDPFGAKPFHPQDGLRHNQYQGLGDSKSDMGTANSKSRTGSLHGALGESKIMDDFGAVPFTELVIHSGPQQQQQQQQAPSQPVELDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_018673659.1
Organism	Lates calcarifer(barramundi perch)
Definition	Lates calcarifer BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018673659.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGCGTCCGGG 
TCCGGGTCCA GCTCCGGAGT CAGCAGCTAC TTCGGGAAAG TGTTCGCCGT CGGCCGGTAT 
CAAGTCACCG TGGAGGAGCT TATTGCAGAA GGAGGCTTCT CGGTGGTGTT TCTGGCTCGT 
ACGCACAGCG GCGTCCGCTG CGCTCTCAAG AGGATGTATG TCAACAACGT CCCGGACCTG 
AACGTCTACA AGAGGGAGAT CACTATCATG AAGGAGCTGT CGGGCCACAA AAACATCGTC 
GGCTACCTGG ACTCCACGAT CAGTCCCGTG TCGGACAGCG TCTGGGAGGT CCTGATCCTC 
ATGGAGTACT GTAAAGCGGG TCAGGTGGTG AAGCAGATGA ACCAGCGGTT GAATGTGGGC 
TTCAGCGAGG CCGAGGTCCT TCACATCTTC TGTGACACCT GTGAGGCCGT GGCTCGCCTG 
CACCAATGCA AGACGCCCGT CATCCACAGA GACCTAAAGG TTGAAAACAT CCTGCTGAAC 
GACCAGGGAA ATTATGTGCT GTGTGACTTT GGAAGTGCCA CTCACAAGGT TTTGCTGCCA 
CATAAAGACG GAGTTACTGC TGTGGAGGAC GAGATTAAGA AGTACACGAC CCTGTCGTAT 
CGGGCGCCTG AGATGATCAA CTTGTACGCA GGGAAAGCCA TCACCACTAA AGCTGATATA 
TGGGCACTTG GATGCTTGTT GTACAAGCTG TGTTTCTTCG CACTTCCATT TGGGGAGAGT 
CAAGTTGCCA TATGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAATT CTCCTTCAAG 
TTGCACTGCT TAATCAGATA TATGCTCGAA CCAGACCAGG AGAAGAGACC AGACATTTAC 
CAAGTGTCCT ACTTTGCCTT CAAGTTGGCT GGAAAAGACT GTCCAGTGCC AAATCTCTTT 
AACTCTCCTA TCCCTACGTC GCTCCCAGAG CCTCTAACGG CCAGCGAGGT CGCTGCTAAG 
AAAAGTATGA CAAAAGCCAG GATAACAGAC TCTGTCGGCC CGACGGAAAC ATCGATAGCT 
CCCAGACAGA GGCCGAAGGC TGCCAACAGT AACGTCCTGC CCCTCGCCAA CACTGTCACC 
CCTGTCAAGA TGACCATGCC CTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACCCCT 
GGCTCCGGGC AGCCGTCCAT GCAGCCCCAG CCTCAGCCCA GCAGTCAGCA GCACCGAGTC 
CTGCAGCAGC TACAGCCTGG CGACCTGCGT CTACAACAGC TACAACAACA TCACCACCAC 
CAACAACAAC AACAACAACA AGCAGTGCAG CAGCAACACG CTAACGCACA GCAACTCCAA 
TACCTCCAGT ACCAACAGGC GGTGCAGCAG TCCCTCCAGC TTCAGCAGCA GCAGGCCCTG 
CTCCAGCAGC AGCAGCAGAT GATGATGCAG CCGATGTACC AGCAGCAGGT TGCTCAGGCC 
CAGGCCCAGG CTCAGGCTCA GGCTCAGGCT CAGGCTCAGT ACGCAGCCAT GCTGCACCAG 
TACCAACAGG CTTTTGTCCA GCAACAGCAG CAACAACAAC AGCATCATCA ACATCATCAA 
CAACAACAAC AACATCAGCA GCAGCAGCAT CATCCCGCTC AGCAGCCTCT CCCCTATATG 
TCCTCCCCTC TTGAGTTCCA GATCCCACTG GGGACCTACA GCGCCACTAC ACCCACCGCA 
GGAACTGGAG CTGGACCTGG TCAGATGGGG GGACTGTCAC CTGTGGATCC CTCATTCACT 
AACCCCAGTA GAAACCCTCT CCTCGCCTCA GATGGGATCA CCCCGCCCTC TCAGAGCTGC 
AACAGTACAG TCAGTAACCC CCCCGACATG TCGGGCTGGA ACCCTTTCGG AGAGGACAAC 
TTCTCCAAGT TGACCGAGGA GGAGCTGATC GACCGGGAGT TCGACATGCT CAGAGCAAAC 
AAACCGGTGG AGAGAACAGC CAGTGTGGAA ACGGACCGAC CGCAGCCGAC CGCCGCCAAG 
CCTCTCCCTC CAGAGGACCT GTTCGGCTCA GTCCCGTTCG TGGCCAGCGC AGGTACCAGT 
GTGATAAAGA TTGAGCAGAC CCCTGAGGAA GTCGCCGTTC CCGCCCCCGT CTCGACCACC 
ACAGACAATC TGCAGCCGAC AGCAGCCAAG GAGCACAAAC CAGGCAGGAA GAGTAACTGT 
AGACCAGGTG AAGAGTCCGA CAGCGACTTC GAGTCTGATC CTCCTTCGCC GAAAAGCAGC 
GAAGATGAAG AGCCGGAGGA GGACGAGGCG CTGAACAGCG AGCACGGTGA AGACACAGAG 
CCGGAGAATT TAGGACAGAG GCCTCTGCTC ATGGACTCAG AGGAAGAGGG CGAGGAGGAC 
GATGACAAGC ACAGCTCTGA CTCGGACTAC GACCCCAGCA GAGTCAAGAC TCGCTCCAAG 
AAACCTGTGG CGGGTAAAGC AGCTTCTGCA GCAACCGGGA GGAGTCCTCG AGGAGATTCT 
GCCATGACCC TGATCACCCC TCCTGAGTCG CCCAGCGGAG CAAGAGCTGC TCCGGTCACG 
GATGATGTGG ATGTTTTCGG TGCCGTTCCC TTCCTCGGAG GAGCCTCACC TGTCGGACCT 
TCAGAGAACA CGGACATCTT TGCCAAAGCG CCTTTCAGGC AAGTCAGCCA CGAGCAGCAA 
GCTCCGGATG AGTTTGATGT TTTTACCAAA GCGCCATTCA ACCGGAACCT CTCCAAGTCT 
GGCAAGAGTG GCAGTGATGT TCCCATGGGC CAAACGCCGC CCATTTCCCC CGAGGGCATT 
GACATTTTCG GCTTCTCTCC CTTCCAGCCG GGCCCCACCG CGCCACCTCC CATCACCTCC 
AGAAGTGCGG ATGATATTTT TCGGCCATCT TACGAGGAGT CGCCGAGTCC TCAGCAGCAG 
AGGCTGAAGC AGCGCAGCCT CCAGAAGCTG TCGTCACGCC AGAGAAAAAC CAAGCAAGAG 
GCCACGGCGG GCGGCAGCAA CGGTAAACGG CATCACGGTA CACCTACTGG AGGGCGCAAG 
ACCAACAAGC CGACTTACCG CACACCAGAG CGAGTCCGCA GGCATAAGAA GGTCGGCCGG 
AGAGACTCGC AGAGCAGCAA CGAGTTCCTC AGCACCTCTG ACTCTAAAGA GAACATCAGT 
GTGGACATAA CCATGTCTGA AGGGAAAGAC AAAGGAGCTT CTCTGCCGGT AGACGAGGCC 
CTTTTAGACC CGTTTGGGGC CAAACCCTTC CATCCTCAGG ACGGATTACG GCACAACCAG 
TATCAAGGCC TCGGTGACAG TAAGAGCGAC ATGGGCACGG CCAACAGCAA GTCCAGGACG 
GGTTCTCTTC ACGGAGCTCT CGGAGAAAGC AAGATCATGG ATGATTTCGG GGCGGTGCCC 
TTCACAGAAC TGGTCATCCA CAGTGGACCT CAGCAGCAGC AGCAGCAGCA GCAGGCGCCC 
TCGCAGCCGG TGGAGCTTGA CCCGTTCGGA GCTGCACCTT TTCCCTCAAA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp2k ( XM_018673660.1 ) cDNA ORF clone, Lates calcarifer(barramundi perch) -> XP_018529176.1 Lates calcarifer BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLa49955
Clone ID Related Accession (Same CDS sequence)	XM_018673660.1
Accession Version	XM_018673660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-13
Organism	Lates calcarifer(barramundi perch)
Product	BMP-2-inducible protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017363863.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lates calcarifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGCGTCCGGG 
TCCGGGTCCA GCTCCGGAGT CAGCAGCTAC TTCGGGAAAG TGTTCGCCGT CGGCCGGTAT 
CAAGTCACCG TGGAGGAGCT TATTGCAGAA GGAGGCTTCT CGGTGGTGTT TCTGGCTCGT 
ACGCACAGCG GCGTCCGCTG CGCTCTCAAG AGGATGTATG TCAACAACGT CCCGGACCTG 
AACGTCTACA AGAGGGAGAT CACTATCATG AAGGAGCTGT CGGGCCACAA AAACATCGTC 
GGCTACCTGG ACTCCACGAT CAGTCCCGTG TCGGACAGCG TCTGGGAGGT CCTGATCCTC 
ATGGAGTACT GTAAAGCGGG TCAGGTGGTG AAGCAGATGA ACCAGCGGTT GAATGTGGGC 
TTCAGCGAGG CCGAGGTCCT TCACATCTTC TGTGACACCT GTGAGGCCGT GGCTCGCCTG 
CACCAATGCA AGACGCCCGT CATCCACAGA GACCTAAAGG TTGAAAACAT CCTGCTGAAC 
GACCAGGGAA ATTATGTGCT GTGTGACTTT GGAAGTGCCA CTCACAAGGT TTTGCTGCCA 
CATAAAGACG GAGTTACTGC TGTGGAGGAC GAGATTAAGA AGTACACGAC CCTGTCGTAT 
CGGGCGCCTG AGATGATCAA CTTGTACGCA GGGAAAGCCA TCACCACTAA AGCTGATATA 
TGGGCACTTG GATGCTTGTT GTACAAGCTG TGTTTCTTCG CACTTCCATT TGGGGAGAGT 
CAAGTTGCCA TATGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAATT CTCCTTCAAG 
TTGCACTGCT TAATCAGATA TATGCTCGAA CCAGACCAGG AGAAGAGACC AGACATTTAC 
CAAGTGTCCT ACTTTGCCTT CAAGTTGGCT GGAAAAGACT GTCCAGTGCC AAATCTCTTT 
AACTCTCCTA TCCCTACGTC GCTCCCAGAG CCTCTAACGG CCAGCGAGGT CGCTGCTAAG 
AAAAGTATGA CAAAAGCCAG GATAACAGAC TCTGTCGGCC CGACGGAAAC ATCGATAGCT 
CCCAGACAGA GGCCGAAGGC TGCCAACAGT AACGTCCTGC CCCTCGCCAA CACTGTCACC 
CCTGTCAAGA TGACCATGCC CTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACCCCT 
GGCTCCGGGC AGCCGTCCAT GCAGCCCCAG CCTCAGCCCA GCAGTCAGCA GCACCGAGTC 
CTGCAGCAGC TACAGCCTGG CGACCTGCGT CTACAACAGC TACAACAACA TCACCACCAC 
CAACAACAAC AACAACAACA AGCAGTGCAG CAGCAACACG CTAACGCACA GCAACTCCAA 
TACCTCCAGT ACCAACAGGC GGTGCAGCAG TCCCTCCAGC TTCAGCAGCA GCAGGCCCTG 
CTCCAGCAGC AGCAGCAGAT GATGATGCAG CCGATGTACC AGCAGCAGGT TGCTCAGGCC 
CAGGCCCAGG CTCAGGCTCA GGCTCAGGCT CAGGCTCAGT ACGCAGCCAT GCTGCACCAG 
TACCAACAGG CTTTTGTCCA GCAACAGCAG CAACAACAAC AGCATCATCA ACATCATCAA 
CAACAACAAC AACATCAGCA GCAGCAGCAT CATCCCGCTC AGCAGCCTCT CCCCTATATG 
TCCTCCCCTC TTGAGTTCCA GATCCCACTG GGGACCTACA GCGCCACTAC ACCCACCGCA 
GGAACTGGAG CTGGACCTGG TCAGATGGGG GGACTGTCAC CTGTGGATCC CTCATTCACT 
AACCCCAGAA ACCCTCTCCT CGCCTCAGAT GGGATCACCC CGCCCTCTCA GAGCTGCAAC 
AGTACAGTCA GTAACCCCCC CGACATGTCG GGCTGGAACC CTTTCGGAGA GGACAACTTC 
TCCAAGTTGA CCGAGGAGGA GCTGATCGAC CGGGAGTTCG ACATGCTCAG AGCAAACAAA 
CCGGTGGAGA GAACAGCCAG TGTGGAAACG GACCGACCGC AGCCGACCGC CGCCAAGCCT 
CTCCCTCCAG AGGACCTGTT CGGCTCAGTC CCGTTCGTGG CCAGCGCAGG TACCAGTGTG 
ATAAAGATTG AGCAGACCCC TGAGGAAGTC GCCGTTCCCG CCCCCGTCTC GACCACCACA 
GACAATCTGC AGCCGACAGC AGCCAAGGAG CACAAACCAG GCAGGAAGAG TAACTGTAGA 
CCAGGTGAAG AGTCCGACAG CGACTTCGAG TCTGATCCTC CTTCGCCGAA AAGCAGCGAA 
GATGAAGAGC CGGAGGAGGA CGAGGCGCTG AACAGCGAGC ACGGTGAAGA CACAGAGCCG 
GAGAATTTAG GACAGAGGCC TCTGCTCATG GACTCAGAGG AAGAGGGCGA GGAGGACGAT 
GACAAGCACA GCTCTGACTC GGACTACGAC CCCAGCAGAG TCAAGACTCG CTCCAAGAAA 
CCTGTGGCGG GTAAAGCAGC TTCTGCAGCA ACCGGGAGGA GTCCTCGAGG AGATTCTGCC 
ATGACCCTGA TCACCCCTCC TGAGTCGCCC AGCGGAGCAA GAGCTGCTCC GGTCACGGAT 
GATGTGGATG TTTTCGGTGC CGTTCCCTTC CTCGGAGGAG CCTCACCTGT CGGACCTTCA 
GAGAACACGG ACATCTTTGC CAAAGCGCCT TTCAGGCAAG TCAGCCACGA GCAGCAAGCT 
CCGGATGAGT TTGATGTTTT TACCAAAGCG CCATTCAACC GGAACCTCTC CAAGTCTGGC 
AAGAGTGGCA GTGATGTTCC CATGGGCCAA ACGCCGCCCA TTTCCCCCGA GGGCATTGAC 
ATTTTCGGCT TCTCTCCCTT CCAGCCGGGC CCCACCGCGC CACCTCCCAT CACCTCCAGA 
AGTGCGGATG ATATTTTTCG GCCATCTTAC GAGGAGTCGC CGAGTCCTCA GCAGCAGAGG 
CTGAAGCAGC GCAGCCTCCA GAAGCTGTCG TCACGCCAGA GAAAAACCAA GCAAGAGGCC 
ACGGCGGGCG GCAGCAACGG TAAACGGCAT CACGGTACAC CTACTGGAGG GCGCAAGACC 
AACAAGCCGA CTTACCGCAC ACCAGAGCGA GTCCGCAGGC ATAAGAAGGT CGGCCGGAGA 
GACTCGCAGA GCAGCAACGA GTTCCTCAGC ACCTCTGACT CTAAAGAGAA CATCAGTGTG 
GACATAACCA TGTCTGAAGG GAAAGACAAA GGAGCTTCTC TGCCGGTAGA CGAGGCCCTT 
TTAGACCCGT TTGGGGCCAA ACCCTTCCAT CCTCAGGACG GATTACGGCA CAACCAGTAT 
CAAGGCCTCG GTGACAGTAA GAGCGACATG GGCACGGCCA ACAGCAAGTC CAGGACGGGT 
TCTCTTCACG GAGCTCTCGG AGAAAGCAAG ATCATGGATG ATTTCGGGGC GGTGCCCTTC 
ACAGAACTGG TCATCCACAG TGGACCTCAG CAGCAGCAGC AGCAGCAGCA GGCGCCCTCG 
CAGCCGGTGG AGCTTGACCC GTTCGGAGCT GCACCTTTTC CCTCAAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018529176.1
CDS	897..4430
Translation	MKKFSRMPKSESGGLGGASGSGSSSGVSSYFGKVFAVGRYQVTVEELIAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNVYKREITIMKELSGHKNIVGYLDSTISPVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPVIHRDLKVENILLNDQGNYVLCDFGSATHKVLLPHKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKAITTKADIWALGCLLYKLCFFALPFGESQVAICDGTFIVPDNSKFSFKLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGKDCPVPNLFNSPIPTSLPEPLTASEVAAKKSMTKARITDSVGPTETSIAPRQRPKAANSNVLPLANTVTPVKMTMPSAPVSNGQKAPTPGSGQPSMQPQPQPSSQQHRVLQQLQPGDLRLQQLQQHHHHQQQQQQQAVQQQHANAQQLQYLQYQQAVQQSLQLQQQQALLQQQQQMMMQPMYQQQVAQAQAQAQAQAQAQAQYAAMLHQYQQAFVQQQQQQQQHHQHHQQQQQHQQQQHHPAQQPLPYMSSPLEFQIPLGTYSATTPTAGTGAGPGQMGGLSPVDPSFTNPRNPLLASDGITPPSQSCNSTVSNPPDMSGWNPFGEDNFSKLTEEELIDREFDMLRANKPVERTASVETDRPQPTAAKPLPPEDLFGSVPFVASAGTSVIKIEQTPEEVAVPAPVSTTTDNLQPTAAKEHKPGRKSNCRPGEESDSDFESDPPSPKSSEDEEPEEDEALNSEHGEDTEPENLGQRPLLMDSEEEGEEDDDKHSSDSDYDPSRVKTRSKKPVAGKAASAATGRSPRGDSAMTLITPPESPSGARAAPVTDDVDVFGAVPFLGGASPVGPSENTDIFAKAPFRQVSHEQQAPDEFDVFTKAPFNRNLSKSGKSGSDVPMGQTPPISPEGIDIFGFSPFQPGPTAPPPITSRSADDIFRPSYEESPSPQQQRLKQRSLQKLSSRQRKTKQEATAGGSNGKRHHGTPTGGRKTNKPTYRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISVDITMSEGKDKGASLPVDEALLDPFGAKPFHPQDGLRHNQYQGLGDSKSDMGTANSKSRTGSLHGALGESKIMDDFGAVPFTELVIHSGPQQQQQQQQAPSQPVELDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_018673660.1
Organism	Lates calcarifer(barramundi perch)
Definition	Lates calcarifer BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018673660.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGAAT GCCAAAGTCC GAAAGCGGCG GGCTCGGAGG GGCGTCCGGG 
TCCGGGTCCA GCTCCGGAGT CAGCAGCTAC TTCGGGAAAG TGTTCGCCGT CGGCCGGTAT 
CAAGTCACCG TGGAGGAGCT TATTGCAGAA GGAGGCTTCT CGGTGGTGTT TCTGGCTCGT 
ACGCACAGCG GCGTCCGCTG CGCTCTCAAG AGGATGTATG TCAACAACGT CCCGGACCTG 
AACGTCTACA AGAGGGAGAT CACTATCATG AAGGAGCTGT CGGGCCACAA AAACATCGTC 
GGCTACCTGG ACTCCACGAT CAGTCCCGTG TCGGACAGCG TCTGGGAGGT CCTGATCCTC 
ATGGAGTACT GTAAAGCGGG TCAGGTGGTG AAGCAGATGA ACCAGCGGTT GAATGTGGGC 
TTCAGCGAGG CCGAGGTCCT TCACATCTTC TGTGACACCT GTGAGGCCGT GGCTCGCCTG 
CACCAATGCA AGACGCCCGT CATCCACAGA GACCTAAAGG TTGAAAACAT CCTGCTGAAC 
GACCAGGGAA ATTATGTGCT GTGTGACTTT GGAAGTGCCA CTCACAAGGT TTTGCTGCCA 
CATAAAGACG GAGTTACTGC TGTGGAGGAC GAGATTAAGA AGTACACGAC CCTGTCGTAT 
CGGGCGCCTG AGATGATCAA CTTGTACGCA GGGAAAGCCA TCACCACTAA AGCTGATATA 
TGGGCACTTG GATGCTTGTT GTACAAGCTG TGTTTCTTCG CACTTCCATT TGGGGAGAGT 
CAAGTTGCCA TATGTGACGG AACCTTTATC GTTCCAGATA ACTCCAAATT CTCCTTCAAG 
TTGCACTGCT TAATCAGATA TATGCTCGAA CCAGACCAGG AGAAGAGACC AGACATTTAC 
CAAGTGTCCT ACTTTGCCTT CAAGTTGGCT GGAAAAGACT GTCCAGTGCC AAATCTCTTT 
AACTCTCCTA TCCCTACGTC GCTCCCAGAG CCTCTAACGG CCAGCGAGGT CGCTGCTAAG 
AAAAGTATGA CAAAAGCCAG GATAACAGAC TCTGTCGGCC CGACGGAAAC ATCGATAGCT 
CCCAGACAGA GGCCGAAGGC TGCCAACAGT AACGTCCTGC CCCTCGCCAA CACTGTCACC 
CCTGTCAAGA TGACCATGCC CTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACCCCT 
GGCTCCGGGC AGCCGTCCAT GCAGCCCCAG CCTCAGCCCA GCAGTCAGCA GCACCGAGTC 
CTGCAGCAGC TACAGCCTGG CGACCTGCGT CTACAACAGC TACAACAACA TCACCACCAC 
CAACAACAAC AACAACAACA AGCAGTGCAG CAGCAACACG CTAACGCACA GCAACTCCAA 
TACCTCCAGT ACCAACAGGC GGTGCAGCAG TCCCTCCAGC TTCAGCAGCA GCAGGCCCTG 
CTCCAGCAGC AGCAGCAGAT GATGATGCAG CCGATGTACC AGCAGCAGGT TGCTCAGGCC 
CAGGCCCAGG CTCAGGCTCA GGCTCAGGCT CAGGCTCAGT ACGCAGCCAT GCTGCACCAG 
TACCAACAGG CTTTTGTCCA GCAACAGCAG CAACAACAAC AGCATCATCA ACATCATCAA 
CAACAACAAC AACATCAGCA GCAGCAGCAT CATCCCGCTC AGCAGCCTCT CCCCTATATG 
TCCTCCCCTC TTGAGTTCCA GATCCCACTG GGGACCTACA GCGCCACTAC ACCCACCGCA 
GGAACTGGAG CTGGACCTGG TCAGATGGGG GGACTGTCAC CTGTGGATCC CTCATTCACT 
AACCCCAGAA ACCCTCTCCT CGCCTCAGAT GGGATCACCC CGCCCTCTCA GAGCTGCAAC 
AGTACAGTCA GTAACCCCCC CGACATGTCG GGCTGGAACC CTTTCGGAGA GGACAACTTC 
TCCAAGTTGA CCGAGGAGGA GCTGATCGAC CGGGAGTTCG ACATGCTCAG AGCAAACAAA 
CCGGTGGAGA GAACAGCCAG TGTGGAAACG GACCGACCGC AGCCGACCGC CGCCAAGCCT 
CTCCCTCCAG AGGACCTGTT CGGCTCAGTC CCGTTCGTGG CCAGCGCAGG TACCAGTGTG 
ATAAAGATTG AGCAGACCCC TGAGGAAGTC GCCGTTCCCG CCCCCGTCTC GACCACCACA 
GACAATCTGC AGCCGACAGC AGCCAAGGAG CACAAACCAG GCAGGAAGAG TAACTGTAGA 
CCAGGTGAAG AGTCCGACAG CGACTTCGAG TCTGATCCTC CTTCGCCGAA AAGCAGCGAA 
GATGAAGAGC CGGAGGAGGA CGAGGCGCTG AACAGCGAGC ACGGTGAAGA CACAGAGCCG 
GAGAATTTAG GACAGAGGCC TCTGCTCATG GACTCAGAGG AAGAGGGCGA GGAGGACGAT 
GACAAGCACA GCTCTGACTC GGACTACGAC CCCAGCAGAG TCAAGACTCG CTCCAAGAAA 
CCTGTGGCGG GTAAAGCAGC TTCTGCAGCA ACCGGGAGGA GTCCTCGAGG AGATTCTGCC 
ATGACCCTGA TCACCCCTCC TGAGTCGCCC AGCGGAGCAA GAGCTGCTCC GGTCACGGAT 
GATGTGGATG TTTTCGGTGC CGTTCCCTTC CTCGGAGGAG CCTCACCTGT CGGACCTTCA 
GAGAACACGG ACATCTTTGC CAAAGCGCCT TTCAGGCAAG TCAGCCACGA GCAGCAAGCT 
CCGGATGAGT TTGATGTTTT TACCAAAGCG CCATTCAACC GGAACCTCTC CAAGTCTGGC 
AAGAGTGGCA GTGATGTTCC CATGGGCCAA ACGCCGCCCA TTTCCCCCGA GGGCATTGAC 
ATTTTCGGCT TCTCTCCCTT CCAGCCGGGC CCCACCGCGC CACCTCCCAT CACCTCCAGA 
AGTGCGGATG ATATTTTTCG GCCATCTTAC GAGGAGTCGC CGAGTCCTCA GCAGCAGAGG 
CTGAAGCAGC GCAGCCTCCA GAAGCTGTCG TCACGCCAGA GAAAAACCAA GCAAGAGGCC 
ACGGCGGGCG GCAGCAACGG TAAACGGCAT CACGGTACAC CTACTGGAGG GCGCAAGACC 
AACAAGCCGA CTTACCGCAC ACCAGAGCGA GTCCGCAGGC ATAAGAAGGT CGGCCGGAGA 
GACTCGCAGA GCAGCAACGA GTTCCTCAGC ACCTCTGACT CTAAAGAGAA CATCAGTGTG 
GACATAACCA TGTCTGAAGG GAAAGACAAA GGAGCTTCTC TGCCGGTAGA CGAGGCCCTT 
TTAGACCCGT TTGGGGCCAA ACCCTTCCAT CCTCAGGACG GATTACGGCA CAACCAGTAT 
CAAGGCCTCG GTGACAGTAA GAGCGACATG GGCACGGCCA ACAGCAAGTC CAGGACGGGT 
TCTCTTCACG GAGCTCTCGG AGAAAGCAAG ATCATGGATG ATTTCGGGGC GGTGCCCTTC 
ACAGAACTGG TCATCCACAG TGGACCTCAG CAGCAGCAGC AGCAGCAGCA GGCGCCCTCG 
CAGCCGGTGG AGCTTGACCC GTTCGGAGCT GCACCTTTTC CCTCAAAACA GTGA