arhgef33 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef33
Entrez Gene ID	109139340
Full Name	Rho guanine nucleotide exchange factor 33
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019262753.2	XP_019118298.2	rho guanine nucleotide exchange factor 33
XM_019262753.1	XP_019118298.1	rho guanine nucleotide exchange factor 33

Pathways from BioSystems
Gene Ontology

The following arhgef33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa217198	XM_019262753.2 Latest version!	Larimichthys crocea Rho guanine nucleotide exchange factor 33 (arhgef33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OLa94191	XM_019262753.1 Latest version!	Larimichthys crocea Rho guanine nucleotide exchange factor 33 (arhgef33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef33 ( XM_019262753.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019118298.2 Larimichthys crocea Rho guanine nucleotide exchange factor 33 (arhgef33), mRNA.

Price & Availability↑

CloneID	OLa217198
Clone ID Related Accession (Same CDS sequence)	XM_019262753.2
Accession Version	XM_019262753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	rho guanine nucleotide exchange factor 33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040013.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019262753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATG GCAAAACAGA AGTAGAAGAC ACGACAGCAG GCATGTCCGA AGGTAAAAAG 
GGTCGTGCTG AAGCTGCGAC TAACCGCGGT GACGGTAAAG AAGAAAATGT TGAGGAGGCT 
AACCTAGAGA TCGCTCAGCT GCAGGGCCTG GTGGCCGAGC TACGCGAGGG GCTCCATGCT 
GCCCTGACGG AGCTGTACGA GCTGCGTCAG AGGGACCATG GCCTGGAGGA GAAACTCCAG 
GCCCATCAGA CTGACGTGGA TGATAAGATA ATGGGCCTCA AGAACTCCCT CAACACTTTC 
AAGGAGGAGC TGAATGTGGC GTTGTTCCAC ATAAAGGATG TTTCCAGCAG ACAGAGAGAG 
GTGCAGAAGA GGATAGAGCT GTTGCAGTCA GAAAACGCCA AAGACGTCAT CTCTGTTCCA 
CACAGAAAAG GCTCGAAGGC AGATGTGCTG ATGGCATCAG GAGATGAACA CATCTGTGTG 
CCCAGCCAGT CAGACCTCAG TGTTATTCAA CACTTCTTCT CCAGCCTGCC GCACAGCGGG 
TCGCCACAGA GGAGCAACAC CACATCGACC TCTACCTCTG AGCAGGATGT TCAGCAGCAG 
CAGAGAGGTT GTCGGTCCAA ATCTCCAGCA TGGGGAGAGA GGAAGAACAG CAGAGACGAC 
ACAGAGACGC CCAACAGCCC GACTGAAAAC AGTAAGAGGC AGAGAGTGGC TCTGGAGCTG 
CTGGAGTCCG AGAGAGTTTA TGTGTCCCAC CTGTCTCTGT TACTGAAGGC CAACATCTCC 
TTTAACGGAT CCGAGGCGCT CACTACCAAA GACAAACGTC CGTTTCCCAG CTCTCTGAGG 
TTTCTAATCC AGCAGCATCT CGAGCTCCTC CACACTCTCC AGGAACGTGT GCTCAAGTGC 
CAGTGGCAAG GCATCATGGG GGATGTGTTT ATGAGGCTCA CCAGCAAAGA GAGTGATTTC 
TTGGACTTTT ATGTGTCGTA CCTGAAGGAG CTTCCAGATT GTCTTTCAGT CGTCACCATG 
CTCGCCTCCA GCTCCATGAA ATCCTCTTCC TTCCTGGAGA GTGACATAAC AGGTGACGAG 
TCCAAACCCT CACTCCACAC TTTGCTCCTT CAGCCGGTTC AGAGGGTCCC GGAGTACCTG 
CTGCTGCTAC AGAAGTTGCT GAGGCAGACA GAGGCAGAGC ACCCAGACTA CTATCTGCTG 
CTGGTGTGCA TCCAGCAGTT CAGGTCGTTT ACAGCTCAGT ACCACCACCT CCTCCAGCAC 
AACCAGGAGC TACTACAGCA CAACCACAAG GAGGTGAAAA GATCTACCAT GAAACAGCTG 
TTAAAGACAG TGGAAAGCGG GATTCAAGCC AACAACATTG GCTCACCATA CCCTTGCAAC 
AGTGCCATGC TAGAACATGC TAACCAGGTG AAGCGGAGCA AGCAGCGTCT CCTGGAGCAG 
ATCCAGTCTC ATCGTTTCCA GGAGTGGGAC CGGGACCAGG ATCCCGAAAA TCATTGTTAC 
GACACAGAGT GGCCCTCTCA GCTCCCATTC TACAGCTCTG ACTTGGACTC CCGGAATCAA 
AAACCAACAG GTCTTGGCAG TATCCCAGAG AGTGAGGCAT CTCAAAGGTC CATGTCGTGC 
CAGCATCAGC TGCCCTCCAG ATCTGCAGAC TTCCGTCAGG TTCAACCAGG CTCTGCACTG 
GCTGATGCCC TTGGTGAATT CCTTCTCCCT CCAGACCCCC CAGGGATAGA GAGCCTCTAT 
GAAGAGGATG GAGGATCATT TCATGATGTC TCCATGTTCG ACCGCTGTTC CTCTGCCTCT 
TCAGATTCCT CCATCGACAT TGCCTTTGTG AAGTGCCCCA AAGCGCCCTT AGTGTCACAT 
CATGCAATGG CAGCGAACGT GTCAAACACC CGTGATGTCT TTGGCAACGG TGGAAGCCAG 
GGGACTGGTT ACAGCAAACT GCCTAACAGA GGGTGCGTAT CTCCAGATGA AGCTGTAATG 
ATGCGCCGCA ATCAGCATCG CCCCCTTCAG GCCAGCCAGC GTAAGAGCAA GTCACTGAAT 
GGCTTGCAAA TGGAGTGCAA CACAGTAAGT GGTCTGGATG GCGGACATCT ATCGGACCAT 
CTCCATAAGG TGGGACTGGG CAGCCACGCC AAGCTGGAGC GCCAGGGCAG TAAAGGCAGC 
AAAGGGTGTC CCACCCCATC CCGTAAGATC CACAGCCCCC TGGGGAACAG AGTGGATACG 
GACAAACAGA GCGACGATCT TCATGGGCTG CTCAGCATTG ACTCCGGTTT CCAATCATGG 
GGAGACGAGT CAAAGTGGAG GGGCGGGACG GAGGAGAATA ACCACACCCC CTTCAGCGAA 
AGGAGTCGGA AGCAGGACAA AGGAGGCTTC AGGAGCTCGT TCAAGAAACT CTTCAAGAAG 
AAGAGCGGCG ATGAGAAGAA AGAGAAGGGA GGTGAGAAAA CACCAGAAAA CCAGAACAAC 
GTTGAACATG AGACGGCAGG GAAAACTCCC AAACTGGTAC ATCTGGAGAT AAACCGTGGC 
ACGGCTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019118298.2
CDS	309..2840
Translation	MENGKTEVEDTTAGMSEGKKGRAEAATNRGDGKEENVEEANLEIAQLQGLVAELREGLHAALTELYELRQRDHGLEEKLQAHQTDVDDKIMGLKNSLNTFKEELNVALFHIKDVSSRQREVQKRIELLQSENAKDVISVPHRKGSKADVLMASGDEHICVPSQSDLSVIQHFFSSLPHSGSPQRSNTTSTSTSEQDVQQQQRGCRSKSPAWGERKNSRDDTETPNSPTENSKRQRVALELLESERVYVSHLSLLLKANISFNGSEALTTKDKRPFPSSLRFLIQQHLELLHTLQERVLKCQWQGIMGDVFMRLTSKESDFLDFYVSYLKELPDCLSVVTMLASSSMKSSSFLESDITGDESKPSLHTLLLQPVQRVPEYLLLLQKLLRQTEAEHPDYYLLLVCIQQFRSFTAQYHHLLQHNQELLQHNHKEVKRSTMKQLLKTVESGIQANNIGSPYPCNSAMLEHANQVKRSKQRLLEQIQSHRFQEWDRDQDPENHCYDTEWPSQLPFYSSDLDSRNQKPTGLGSIPESEASQRSMSCQHQLPSRSADFRQVQPGSALADALGEFLLPPDPPGIESLYEEDGGSFHDVSMFDRCSSASSDSSIDIAFVKCPKAPLVSHHAMAANVSNTRDVFGNGGSQGTGYSKLPNRGCVSPDEAVMMRRNQHRPLQASQRKSKSLNGLQMECNTVSGLDGGHLSDHLHKVGLGSHAKLERQGSKGSKGCPTPSRKIHSPLGNRVDTDKQSDDLHGLLSIDSGFQSWGDESKWRGGTEENNHTPFSERSRKQDKGGFRSSFKKLFKKKSGDEKKEKGGEKTPENQNNVEHETAGKTPKLVHLEINRGTAV

Target ORF information:

RefSeq Version	XM_019262753.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea Rho guanine nucleotide exchange factor 33 (arhgef33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019262753.2

ORF Insert Sequence:

ATGGAGAATG GCAAAACAGA AGTAGAAGAC ACGACAGCAG GCATGTCCGA AGGTAAAAAG 
GGTCGTGCTG AAGCTGCGAC TAACCGCGGT GACGGTAAAG AAGAAAATGT TGAGGAGGCT 
AACCTAGAGA TCGCTCAGCT GCAGGGCCTG GTGGCCGAGC TACGCGAGGG GCTCCATGCT 
GCCCTGACGG AGCTGTACGA GCTGCGTCAG AGGGACCATG GCCTGGAGGA GAAACTCCAG 
GCCCATCAGA CTGACGTGGA TGATAAGATA ATGGGCCTCA AGAACTCCCT CAACACTTTC 
AAGGAGGAGC TGAATGTGGC GTTGTTCCAC ATAAAGGATG TTTCCAGCAG ACAGAGAGAG 
GTGCAGAAGA GGATAGAGCT GTTGCAGTCA GAAAACGCCA AAGACGTCAT CTCTGTTCCA 
CACAGAAAAG GCTCGAAGGC AGATGTGCTG ATGGCATCAG GAGATGAACA CATCTGTGTG 
CCCAGCCAGT CAGACCTCAG TGTTATTCAA CACTTCTTCT CCAGCCTGCC GCACAGCGGG 
TCGCCACAGA GGAGCAACAC CACATCGACC TCTACCTCTG AGCAGGATGT TCAGCAGCAG 
CAGAGAGGTT GTCGGTCCAA ATCTCCAGCA TGGGGAGAGA GGAAGAACAG CAGAGACGAC 
ACAGAGACGC CCAACAGCCC GACTGAAAAC AGTAAGAGGC AGAGAGTGGC TCTGGAGCTG 
CTGGAGTCCG AGAGAGTTTA TGTGTCCCAC CTGTCTCTGT TACTGAAGGC CAACATCTCC 
TTTAACGGAT CCGAGGCGCT CACTACCAAA GACAAACGTC CGTTTCCCAG CTCTCTGAGG 
TTTCTAATCC AGCAGCATCT CGAGCTCCTC CACACTCTCC AGGAACGTGT GCTCAAGTGC 
CAGTGGCAAG GCATCATGGG GGATGTGTTT ATGAGGCTCA CCAGCAAAGA GAGTGATTTC 
TTGGACTTTT ATGTGTCGTA CCTGAAGGAG CTTCCAGATT GTCTTTCAGT CGTCACCATG 
CTCGCCTCCA GCTCCATGAA ATCCTCTTCC TTCCTGGAGA GTGACATAAC AGGTGACGAG 
TCCAAACCCT CACTCCACAC TTTGCTCCTT CAGCCGGTTC AGAGGGTCCC GGAGTACCTG 
CTGCTGCTAC AGAAGTTGCT GAGGCAGACA GAGGCAGAGC ACCCAGACTA CTATCTGCTG 
CTGGTGTGCA TCCAGCAGTT CAGGTCGTTT ACAGCTCAGT ACCACCACCT CCTCCAGCAC 
AACCAGGAGC TACTACAGCA CAACCACAAG GAGGTGAAAA GATCTACCAT GAAACAGCTG 
TTAAAGACAG TGGAAAGCGG GATTCAAGCC AACAACATTG GCTCACCATA CCCTTGCAAC 
AGTGCCATGC TAGAACATGC TAACCAGGTG AAGCGGAGCA AGCAGCGTCT CCTGGAGCAG 
ATCCAGTCTC ATCGTTTCCA GGAGTGGGAC CGGGACCAGG ATCCCGAAAA TCATTGTTAC 
GACACAGAGT GGCCCTCTCA GCTCCCATTC TACAGCTCTG ACTTGGACTC CCGGAATCAA 
AAACCAACAG GTCTTGGCAG TATCCCAGAG AGTGAGGCAT CTCAAAGGTC CATGTCGTGC 
CAGCATCAGC TGCCCTCCAG ATCTGCAGAC TTCCGTCAGG TTCAACCAGG CTCTGCACTG 
GCTGATGCCC TTGGTGAATT CCTTCTCCCT CCAGACCCCC CAGGGATAGA GAGCCTCTAT 
GAAGAGGATG GAGGATCATT TCATGATGTC TCCATGTTCG ACCGCTGTTC CTCTGCCTCT 
TCAGATTCCT CCATCGACAT TGCCTTTGTG AAGTGCCCCA AAGCGCCCTT AGTGTCACAT 
CATGCAATGG CAGCGAACGT GTCAAACACC CGTGATGTCT TTGGCAACGG TGGAAGCCAG 
GGGACTGGTT ACAGCAAACT GCCTAACAGA GGGTGCGTAT CTCCAGATGA AGCTGTAATG 
ATGCGCCGCA ATCAGCATCG CCCCCTTCAG GCCAGCCAGC GTAAGAGCAA GTCACTGAAT 
GGCTTGCAAA TGGAGTGCAA CACAGTAAGT GGTCTGGATG GCGGACATCT ATCGGACCAT 
CTCCATAAGG TGGGACTGGG CAGCCACGCC AAGCTGGAGC GCCAGGGCAG TAAAGGCAGC 
AAAGGGTGTC CCACCCCATC CCGTAAGATC CACAGCCCCC TGGGGAACAG AGTGGATACG 
GACAAACAGA GCGACGATCT TCATGGGCTG CTCAGCATTG ACTCCGGTTT CCAATCATGG 
GGAGACGAGT CAAAGTGGAG GGGCGGGACG GAGGAGAATA ACCACACCCC CTTCAGCGAA 
AGGAGTCGGA AGCAGGACAA AGGAGGCTTC AGGAGCTCGT TCAAGAAACT CTTCAAGAAG 
AAGAGCGGCG ATGAGAAGAA AGAGAAGGGA GGTGAGAAAA CACCAGAAAA CCAGAACAAC 
GTTGAACATG AGACGGCAGG GAAAACTCCC AAACTGGTAC ATCTGGAGAT AAACCGTGGC 
ACGGCTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef33 ( XM_019262753.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019118298.1 Larimichthys crocea Rho guanine nucleotide exchange factor 33 (arhgef33), mRNA.

Price & Availability↑

CloneID	OLa94191
Clone ID Related Accession (Same CDS sequence)	XM_019262753.1
Accession Version	XM_019262753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	rho guanine nucleotide exchange factor 33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608502.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATG GCAAAACAGA AGTAGAAGAC ACGACAGCAG GCATGTCCGA AGGTAAAAAG 
GGTCGTGCTG AAGCTGCGAC TAACCGCGGT GACGGTAAAG AAGAAAATGT TGAGGAGGCT 
AACCTAGAGA TCGCTCAGCT GCAGGGCCTG GTGGCCGAGC TACGCGAGGG GCTCCATGCT 
GCCCTGACGG AGCTGTACGA GCTGCGTCAG AGGGACCATG GCCTGGAGGA GAAACTCCAG 
GCCCATCAGA CTGACGTGGA TGATAAGATA ATGGGCCTCA AGAACTCCCT CAACACTTTC 
AAGGAGGAGC TGAATGTGGC GTTGTTCCAC ATAAAGGATG TTTCCAGCAG ACAGAGAGAG 
GTGCAGAAGA GGATAGAGCT GTTGCAGTCA GAAAACGCCA AAGACGTCAT CTCTGTTCCA 
CACAGAAAAG GCTCGAAGGC AGATGTGCTG ATGGCATCAG GAGATGAACA CATCTGTGTG 
CCCAGCCAGT CAGACCTCAG TGTTATTCAA CACTTCTTCT CCAGCCTGCC ACACAGCGGG 
TCGCCACAGA GGAGCAACAC CACGTCGACC TCTACCTCTG AGCAGGATGT TCAGCAGCAG 
CAGCAGAGAG GTTGTCGGTC CAAATCTCCA GCATGGGGAG AGAGGAAGAA CAGCAGAGAC 
GACACAGAGA CGCCCAACAG CCCGACTGAA AACAGTAAGA GGCAGAGAGT GGCTCTGGAG 
CTGCTGGAGT CCGAGAGAGT TTATGTGTCC CACCTGTCTC TGTTACTGAA GGCCAACATC 
TCCTTTAACG GATCCGAGGC GCTCACTACC AAAGACAAAC GTCCGTTTCC CAGCTCTCTG 
AGGTTTCTAA TCCAGCAGCA TCTCGAGCTC CTCCACACTC TCCAGGAACG TGTGCTCAAG 
TGCCAGTGGC AAGGCATCAT GGGGGATGTG TTTATGAGGC TCACCAGCAA AGAGAGTGAT 
TTCTTGGACT TTTATGTGTC GTACCTGAAG GAGCTTCCAG ATTGTCTTTC AGTCGTCACC 
ATGCTCGCCT CCAGCTCCAT GAAATCCTCT TCCTTCCTGG AGAGTGACAT AACAGGTGAC 
GAGTCCAAAC CCTCACTCCA CACTTTGCTC CTTCAGCCGG TTCAGAGGGT CCCGGAGTAC 
CTGCTGCTGC TACAGAAGTT GCTGAGGCAG ACAGAGGCAG AGCACCCAGA CTATTATCTG 
CTGCTGGTGT GCATCCAGCA GTTCAGGTCG TTTACAGCTC AGTACCACCA CCTCCTCCAG 
CACAACCAGG AGCTACTACA GCACAACCAC AAGGAGGTGA AAAGATCTAC CATGAAACAG 
CTGTTAAAGA CAGTGGAAAG CGGGATTCAA GCCAACAACA TTGGCTCACC ATACCCTTGC 
AACAGTGCCA TGCTAGAACA TGCTAACCAG GTGAAGCGGA GCAAGCAGCG TCTCCTGGAG 
CAGATCCAGT CTCATCGTTT CCAGGAGTGG GACCGGGACC AGGATCCCGA AAATCATTGT 
TACGACACAG AGTGGCCCTC TCAGCTCCCA TTCTACAGCT CTGACTTGGA CTCCCGGAAT 
CAAAAACCAA CAGGTCTTGG CAGTATCCCA GAGAGTGAGG CATCTCAGAG GTCCATGTCG 
TGCCAGCATC AGCTGCCCTC CAGATCCGCA GACTTCCGTC AGGTTCAACC AGGCTCTGCA 
CTGGCTGATG CCCTTGGTGA ATTCCTTCTC CCTCCAGACC CCCCAGGGAT AGAGAGCCTC 
TATGAAGAGG ATGGAGGATC ATTTCATGAT GTCTCCATGT TCGACCGCTG TTCCTCTGCC 
TCTTCAGATT CCTCCATCGA CATTGCCTTT GTGAAGTGCC CCAAAGCGCC CTTAGTGTCA 
CATCATGCAA TGGCGGCGAA CGTGTCAAAC ACCCGTGATG TCTTTGGAAA CGGTGGAAGC 
CAGGGGACTG GTTACAGCAA ACTGCCTAAC AGAGGGTGCG TATCTCCAGA TGAAGCTGTA 
ATGATGCGCC GCAATCAGCA TCGCCCCCTT CAGGCCAGCC AGCGTAAGAG CAAGTCACTG 
AATGGCTTGC AAATGGAGTG CAACACAGTT AGTGGTCTGG ATGGCGGACA TCTATCGGAC 
CATCTCCATA AGGTGGGACT GGGCAGCCAC GCCAAGCTGG AGCGCCAGGG CAGTAAAGGC 
AGCAAAGGGT GTCCCACCCC ATCCCGTAAG ATCCACAGCC CCCTGGGGAA CAGAGTGGAT 
ACGGACAAAC AGAGCGACGA TCTTCATGGG CTGCTCAGCA TTGACTCCGG TTTCCAATCA 
TGGGGAGACG AGTCAAAGTG GAGGGGCGGG ACGGAGGAGA ATAACCACAC CCCCTTCAGC 
GAAAGGAGTC GGAAGCAGGA CAAAGGAGGC TTCAGGAGCT CGTTCAAGAA ACTCTTCAAG 
AAGAAGAGCG GCGATGAGAA GAAAGAGAAG GGAGGTGAGA AAACACCAGA AAACCAGAAC 
AACGTTGAAC ATGAGACGGC AGGGAAAACT CCCAAACTGG TACATCTGGA GATAAACCGT 
GGCACGGCTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019118298.1
CDS	290..2824
Translation	MENGKTEVEDTTAGMSEGKKGRAEAATNRGDGKEENVEEANLEIAQLQGLVAELREGLHAALTELYELRQRDHGLEEKLQAHQTDVDDKIMGLKNSLNTFKEELNVALFHIKDVSSRQREVQKRIELLQSENAKDVISVPHRKGSKADVLMASGDEHICVPSQSDLSVIQHFFSSLPHSGSPQRSNTTSTSTSEQDVQQQQQRGCRSKSPAWGERKNSRDDTETPNSPTENSKRQRVALELLESERVYVSHLSLLLKANISFNGSEALTTKDKRPFPSSLRFLIQQHLELLHTLQERVLKCQWQGIMGDVFMRLTSKESDFLDFYVSYLKELPDCLSVVTMLASSSMKSSSFLESDITGDESKPSLHTLLLQPVQRVPEYLLLLQKLLRQTEAEHPDYYLLLVCIQQFRSFTAQYHHLLQHNQELLQHNHKEVKRSTMKQLLKTVESGIQANNIGSPYPCNSAMLEHANQVKRSKQRLLEQIQSHRFQEWDRDQDPENHCYDTEWPSQLPFYSSDLDSRNQKPTGLGSIPESEASQRSMSCQHQLPSRSADFRQVQPGSALADALGEFLLPPDPPGIESLYEEDGGSFHDVSMFDRCSSASSDSSIDIAFVKCPKAPLVSHHAMAANVSNTRDVFGNGGSQGTGYSKLPNRGCVSPDEAVMMRRNQHRPLQASQRKSKSLNGLQMECNTVSGLDGGHLSDHLHKVGLGSHAKLERQGSKGSKGCPTPSRKIHSPLGNRVDTDKQSDDLHGLLSIDSGFQSWGDESKWRGGTEENNHTPFSERSRKQDKGGFRSSFKKLFKKKSGDEKKEKGGEKTPENQNNVEHETAGKTPKLVHLEINRGTAV

Target ORF information:

RefSeq Version	XM_019262753.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea Rho guanine nucleotide exchange factor 33 (arhgef33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019262753.1

ORF Insert Sequence:

ATGGAGAATG GCAAAACAGA AGTAGAAGAC ACGACAGCAG GCATGTCCGA AGGTAAAAAG 
GGTCGTGCTG AAGCTGCGAC TAACCGCGGT GACGGTAAAG AAGAAAATGT TGAGGAGGCT 
AACCTAGAGA TCGCTCAGCT GCAGGGCCTG GTGGCCGAGC TACGCGAGGG GCTCCATGCT 
GCCCTGACGG AGCTGTACGA GCTGCGTCAG AGGGACCATG GCCTGGAGGA GAAACTCCAG 
GCCCATCAGA CTGACGTGGA TGATAAGATA ATGGGCCTCA AGAACTCCCT CAACACTTTC 
AAGGAGGAGC TGAATGTGGC GTTGTTCCAC ATAAAGGATG TTTCCAGCAG ACAGAGAGAG 
GTGCAGAAGA GGATAGAGCT GTTGCAGTCA GAAAACGCCA AAGACGTCAT CTCTGTTCCA 
CACAGAAAAG GCTCGAAGGC AGATGTGCTG ATGGCATCAG GAGATGAACA CATCTGTGTG 
CCCAGCCAGT CAGACCTCAG TGTTATTCAA CACTTCTTCT CCAGCCTGCC ACACAGCGGG 
TCGCCACAGA GGAGCAACAC CACGTCGACC TCTACCTCTG AGCAGGATGT TCAGCAGCAG 
CAGCAGAGAG GTTGTCGGTC CAAATCTCCA GCATGGGGAG AGAGGAAGAA CAGCAGAGAC 
GACACAGAGA CGCCCAACAG CCCGACTGAA AACAGTAAGA GGCAGAGAGT GGCTCTGGAG 
CTGCTGGAGT CCGAGAGAGT TTATGTGTCC CACCTGTCTC TGTTACTGAA GGCCAACATC 
TCCTTTAACG GATCCGAGGC GCTCACTACC AAAGACAAAC GTCCGTTTCC CAGCTCTCTG 
AGGTTTCTAA TCCAGCAGCA TCTCGAGCTC CTCCACACTC TCCAGGAACG TGTGCTCAAG 
TGCCAGTGGC AAGGCATCAT GGGGGATGTG TTTATGAGGC TCACCAGCAA AGAGAGTGAT 
TTCTTGGACT TTTATGTGTC GTACCTGAAG GAGCTTCCAG ATTGTCTTTC AGTCGTCACC 
ATGCTCGCCT CCAGCTCCAT GAAATCCTCT TCCTTCCTGG AGAGTGACAT AACAGGTGAC 
GAGTCCAAAC CCTCACTCCA CACTTTGCTC CTTCAGCCGG TTCAGAGGGT CCCGGAGTAC 
CTGCTGCTGC TACAGAAGTT GCTGAGGCAG ACAGAGGCAG AGCACCCAGA CTATTATCTG 
CTGCTGGTGT GCATCCAGCA GTTCAGGTCG TTTACAGCTC AGTACCACCA CCTCCTCCAG 
CACAACCAGG AGCTACTACA GCACAACCAC AAGGAGGTGA AAAGATCTAC CATGAAACAG 
CTGTTAAAGA CAGTGGAAAG CGGGATTCAA GCCAACAACA TTGGCTCACC ATACCCTTGC 
AACAGTGCCA TGCTAGAACA TGCTAACCAG GTGAAGCGGA GCAAGCAGCG TCTCCTGGAG 
CAGATCCAGT CTCATCGTTT CCAGGAGTGG GACCGGGACC AGGATCCCGA AAATCATTGT 
TACGACACAG AGTGGCCCTC TCAGCTCCCA TTCTACAGCT CTGACTTGGA CTCCCGGAAT 
CAAAAACCAA CAGGTCTTGG CAGTATCCCA GAGAGTGAGG CATCTCAGAG GTCCATGTCG 
TGCCAGCATC AGCTGCCCTC CAGATCCGCA GACTTCCGTC AGGTTCAACC AGGCTCTGCA 
CTGGCTGATG CCCTTGGTGA ATTCCTTCTC CCTCCAGACC CCCCAGGGAT AGAGAGCCTC 
TATGAAGAGG ATGGAGGATC ATTTCATGAT GTCTCCATGT TCGACCGCTG TTCCTCTGCC 
TCTTCAGATT CCTCCATCGA CATTGCCTTT GTGAAGTGCC CCAAAGCGCC CTTAGTGTCA 
CATCATGCAA TGGCGGCGAA CGTGTCAAAC ACCCGTGATG TCTTTGGAAA CGGTGGAAGC 
CAGGGGACTG GTTACAGCAA ACTGCCTAAC AGAGGGTGCG TATCTCCAGA TGAAGCTGTA 
ATGATGCGCC GCAATCAGCA TCGCCCCCTT CAGGCCAGCC AGCGTAAGAG CAAGTCACTG 
AATGGCTTGC AAATGGAGTG CAACACAGTT AGTGGTCTGG ATGGCGGACA TCTATCGGAC 
CATCTCCATA AGGTGGGACT GGGCAGCCAC GCCAAGCTGG AGCGCCAGGG CAGTAAAGGC 
AGCAAAGGGT GTCCCACCCC ATCCCGTAAG ATCCACAGCC CCCTGGGGAA CAGAGTGGAT 
ACGGACAAAC AGAGCGACGA TCTTCATGGG CTGCTCAGCA TTGACTCCGG TTTCCAATCA 
TGGGGAGACG AGTCAAAGTG GAGGGGCGGG ACGGAGGAGA ATAACCACAC CCCCTTCAGC 
GAAAGGAGTC GGAAGCAGGA CAAAGGAGGC TTCAGGAGCT CGTTCAAGAA ACTCTTCAAG 
AAGAAGAGCG GCGATGAGAA GAAAGAGAAG GGAGGTGAGA AAACACCAGA AAACCAGAAC 
AACGTTGAAC ATGAGACGGC AGGGAAAACT CCCAAACTGG TACATCTGGA GATAAACCGT 
GGCACGGCTG TATGA