LOC104937879 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104937879
Entrez Gene ID	104937879
Full Name	sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010754192.2	XP_010752494.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like
XM_010754192.3	XP_010752494.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like

Pathways from BioSystems
Gene Ontology

The following LOC104937879 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104937879 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa102551	XM_010754192.3 Latest version!	Larimichthys crocea sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like (LOC104937879), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
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OLa102551	XM_010754192.2	Larimichthys crocea sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like (LOC104937879), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104937879 ( XM_010754192.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010752494.1 Larimichthys crocea sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like (LOC104937879), mRNA.

Price & Availability↑

CloneID	OLa102551
Clone ID Related Accession (Same CDS sequence)	XM_010754192.2 , XM_010754192.3
Accession Version	XM_010754192.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608999.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010754192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAATG CTCATACTAA GACGGTGGAG GAGGTTTTAG GCTTCTTTGG TGTAAACGAG 
TCCACAGGTC TGAGCTGTGA GCAGCTGAAG AAGAACAGGG AGAGATGGGG ACCCAATGAA 
TTGCCAGCTG AAGAAGGGAA GTCACTTTGG GAGCTGGTCC TGGAACAGTT TGAGGATTTG 
TTGGTTCGAA TCTTGCTGCT GGCTGCATGC ATCTCCTTTA CTCTCGCTTG GTTTGAAGAA 
GGGGAAGGGA CGATCACAGC CTTCGTCGAA CCTTTCGTCA TTCTCCTCAT CCTCATTGCC 
AATGCTATTG TAGGCGTGTG GCAGGAGCGT AATGCAGAGA ATGCCATTGA GGCTCTAAAG 
GAGTATGAGC CAGAGATGGG CAAAGTGTAC CGACAAGACA AGAAGAGCGT TCAGAGGGTC 
AGAGCCAGAG ACATCGTCCC TGGAGACATC GTGGAAGTTG CTGTTGGTGA TAAGGTTCCA 
GCTGACATCA GGCTGACGTC TATCCGATCC ACCACTCTGA GGGTGGACCA GTCCATTCTG 
ACGGGGGAGT CTGTGTCAGT GCTCAAACAC ACAGATCCTG TACCAGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTCTTCTCT GGTACCAACA TTGCAGCGGG GCGAGCCATC 
GGGGTTGTTG TGGCGACAGG GGTGCAGACA GAGATTGGGA AGATCAGAGA TGAAATGGCG 
TCCACTGACC CTGAAAGGAC CCCACTACAA CAGAAACTAG ACCAGTTTGG AGAACAGCTG 
TCTAAGGTCA TCAGTGTGAT CTGTGTGGCA GTGTGGGCCA TCAATGTAGG ACACTTTAAT 
GATCCAGTCC ATGGAGGCAG CTGGCTGAGA GGAGCTGTTT ACTACTTTAA GATCGCTGTT 
GCACTGGCTG TAGCTGCCAT ACCAGAAGGG CTCCCAGCAG TCATCACTAC CTGCCTGGCT 
CTGGGCACCA GGAGGATGGC CAGAAAGAAC GCCATCGTTC GCAGCCTGCC ATCAGTGGAG 
ACGCTGGGCT GCACCTCTGT CATCTGCTCT GACAAGACGG GGACGCTGAC CACCAACCAG 
ATGTCTGTCT GCAGGATGTT TGTTGTCGAC AGTGTGTCAG GGCAGTGCTG CAGACTCAGC 
GAGTTCTCAG TCACTGGATC TACGTACGCC CCTGAAGGAG AAGTTTATAA GGACGGCTCA 
CTGGTGAAGT GCAGTCAGTA TGAAGGCCTG GTGGAGATGG CCTCCATCTG TGCGCTGTGC 
AATGACTCAT CACTGGACTA CAATGAGGCA AAGGGTGTGT ATGAGAAGGT AGGGGAGGCA 
ACTGAGACTG CCCTCTGCTG TCTGGTGGAG AAAATGAACG TGTTTGACAC GGACCTGAGA 
GGACTGAGCC CTGCTGAGAG AGCTACAGCA TGCTGCTCTG TGATTAAGCA GCTCATGAGG 
AAAGAGCTGA CGCTGGAGTT TTCCAGAGAC AGGAAGTCCA TGTCTGTCTT CTGTTCATCC 
AATAAACTCA CCAGGTCTGC CTCTGGAGCC AAGATGTTCA TCAAAGGAGC TCCAGAGAGT 
GTGTTGGAGC GCTGTAATCA TATCCGGATC AGCGGTTCTG CCTGTGTGCC TTTGACCCCG 
GAGGTTCGAG AGCAGCTCCT GTCTAGAGTG CGGGAATGGG GATCAGGCCG AGACACACTG 
CGCTGCCTCG CGATGGCAAC CAGGGACACG CCCCCTGACA TTCACTGCCT CAACCTGGAG 
AACTCGGCTG CCTTTGCAAA CTATGAGACG GACCTGACCT TTGTGGGCTG TGTGGGCATG 
TTGGACCCCC CCAGGAAAGA AGTGCTCGGC GCAGTTCGAA TGTGTCGGCA GGCTGGCATA 
AGAGTCATCA TGATCACAGG TGACAACAAA GGGACGGCCC TGTCTATTTG TCGGAGGGTG 
GGCATCATCA CAGAGCAGGA AGAGGAGTTG GAGGGTGGCG GGGTAAACGG GGGTCTGACA 
GGACGAGAGT TTGATGAGCT GCCCCCGCAT CTGCAGCGTC AGGCCTGCAG GACGGTAAGG 
TGCTTCGCCC GGGTGGAACC AGCACACAAG AGCCGTATAG TGGAGTACCT GCAGAGCCTG 
AGTGACATCA CCGCCATGAC AGGTGACGGT GTGAATGATG CTCCAGCGTT AAAGAAGGCA 
GAGATTGGCA TCGCTATGGG CAGCGGCACG GCAGTGGCCA AGTCGGCCTC TGAGATGATC 
CTGGCCGATG ACAACTTCTC CACCATCGTG GCTGCAGTGG AGGAGGGCAG AGCCATTTAC 
AACAACATGA AGCAGTTCAT CAGATACCTC ATATCATCCA ACATAGGAGA GGTGGTCTGT 
ATCTTCCTGA CGGCTGCGCT GGGTATGCCT GAAGCGCTGA TCCCAGTCCA GCTGCTGTGG 
GTCAACCTGG TCACTGACGG TTTCCCAGCA ACAGCTCTGG GCTTCAACCC TCCAGACCTG 
GACATCATGT CCCGCCCTCC CCGCTCCCCC AAAGAGCCTC TGATCTCCGG CTGGTTGTTC 
TGTCGCTATC TCATCATTGG ATGTTATGTG GGAGCAGCCA CTGTGGGAGC TGCTGCCTGG 
TGGTTTATGG CAGCCCACGA TGGACCAAAA CTTTCCTTCT ATCAACTGTC CCACTACCTG 
CAGTGCAGTG AAGGCCACGC TGAGTTTGCT GGTGTGCAGT GTTCAGTCTT TGAGTCTCCT 
TATCCCATGA CTATGGCCCT GTCTGTTCTG GTTACCATAG AGATGTGCAA CGCCTTGAAC 
AGTCTTTCAG AGAACCAGTC CCTGCTGAAG ATGCCTCCCT GGTCAAACCC TTGGCTGGTG 
GGGGCGATCT GTCTGTCCAT GGCTCTACAT TTCCTCATTC TCTATGTAGA CCCACTGCCA 
GTGATCTTCC AGATCCGCCC TCTGTCGTGG CCTCAGTGGG TGGTGGTGCT GAAAATGTCC 
ATTCCTGTCA TCTTGATGGA CGAGGTTCTT AAATTTCTGG CCCGCAACTA CATCGAGCCA 
GGAAGCCAGA TCCAGTCACT GGAAGAGGAA GAGGAAGAGG AGATGCAGAG GACAAGGGGT 
AACGTGGGAT TGGTCGGTGC CGTGATGACC AGGCTGTCTC AGTCAGTGAA GGGTGTGTCC 
TGGTCATTCG TCCTGGTCTC TGCGCCGCTA GTGTTCTGGA TCTTTAGCCT GGACTCTGAC 
ATAACCAACA TCTTTTGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010752494.1
CDS	424..3627
Translation	MDNAHTKTVEEVLGFFGVNESTGLSCEQLKKNRERWGPNELPAEEGKSLWELVLEQFEDLLVRILLLAACISFTLAWFEEGEGTITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDKKSVQRVRARDIVPGDIVEVAVGDKVPADIRLTSIRSTTLRVDQSILTGESVSVLKHTDPVPDPRAVNQDKKNMLFSGTNIAAGRAIGVVVATGVQTEIGKIRDEMASTDPERTPLQQKLDQFGEQLSKVISVICVAVWAINVGHFNDPVHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFVVDSVSGQCCRLSEFSVTGSTYAPEGEVYKDGSLVKCSQYEGLVEMASICALCNDSSLDYNEAKGVYEKVGEATETALCCLVEKMNVFDTDLRGLSPAERATACCSVIKQLMRKELTLEFSRDRKSMSVFCSSNKLTRSASGAKMFIKGAPESVLERCNHIRISGSACVPLTPEVREQLLSRVREWGSGRDTLRCLAMATRDTPPDIHCLNLENSAAFANYETDLTFVGCVGMLDPPRKEVLGAVRMCRQAGIRVIMITGDNKGTALSICRRVGIITEQEEELEGGGVNGGLTGREFDELPPHLQRQACRTVRCFARVEPAHKSRIVEYLQSLSDITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSASEMILADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNIGEVVCIFLTAALGMPEALIPVQLLWVNLVTDGFPATALGFNPPDLDIMSRPPRSPKEPLISGWLFCRYLIIGCYVGAATVGAAAWWFMAAHDGPKLSFYQLSHYLQCSEGHAEFAGVQCSVFESPYPMTMALSVLVTIEMCNALNSLSENQSLLKMPPWSNPWLVGAICLSMALHFLILYVDPLPVIFQIRPLSWPQWVVVLKMSIPVILMDEVLKFLARNYIEPGSQIQSLEEEEEEEMQRTRGNVGLVGAVMTRLSQSVKGVSWSFVLVSAPLVFWIFSLDSDITNIFWE

Target ORF information:

RefSeq Version	XM_010754192.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like (LOC104937879), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010754192.2

ORF Insert Sequence:

ATGGATAATG CTCATACTAA GACGGTGGAG GAGGTTTTAG GCTTCTTTGG TGTAAACGAG 
TCCACAGGTC TGAGCTGTGA GCAGCTGAAG AAGAACAGGG AGAGATGGGG ACCCAATGAA 
TTGCCAGCTG AAGAAGGGAA GTCACTTTGG GAGCTGGTCC TGGAACAGTT TGAGGATTTG 
TTGGTTCGAA TCTTGCTGCT GGCTGCATGC ATCTCCTTTA CTCTCGCTTG GTTTGAAGAA 
GGGGAAGGGA CGATCACAGC CTTCGTCGAA CCTTTCGTCA TTCTCCTCAT CCTCATTGCC 
AATGCTATTG TAGGCGTGTG GCAGGAGCGT AATGCAGAGA ATGCCATTGA GGCTCTAAAG 
GAGTATGAGC CAGAGATGGG CAAAGTGTAC CGACAAGACA AGAAGAGCGT TCAGAGGGTC 
AGAGCCAGAG ACATCGTCCC TGGAGACATC GTGGAAGTTG CTGTTGGTGA TAAGGTTCCA 
GCTGACATCA GGCTGACGTC TATCCGATCC ACCACTCTGA GGGTGGACCA GTCCATTCTG 
ACGGGGGAGT CTGTGTCAGT GCTCAAACAC ACAGATCCTG TACCAGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTCTTCTCT GGTACCAACA TTGCAGCGGG GCGAGCCATC 
GGGGTTGTTG TGGCGACAGG GGTGCAGACA GAGATTGGGA AGATCAGAGA TGAAATGGCG 
TCCACTGACC CTGAAAGGAC CCCACTACAA CAGAAACTAG ACCAGTTTGG AGAACAGCTG 
TCTAAGGTCA TCAGTGTGAT CTGTGTGGCA GTGTGGGCCA TCAATGTAGG ACACTTTAAT 
GATCCAGTCC ATGGAGGCAG CTGGCTGAGA GGAGCTGTTT ACTACTTTAA GATCGCTGTT 
GCACTGGCTG TAGCTGCCAT ACCAGAAGGG CTCCCAGCAG TCATCACTAC CTGCCTGGCT 
CTGGGCACCA GGAGGATGGC CAGAAAGAAC GCCATCGTTC GCAGCCTGCC ATCAGTGGAG 
ACGCTGGGCT GCACCTCTGT CATCTGCTCT GACAAGACGG GGACGCTGAC CACCAACCAG 
ATGTCTGTCT GCAGGATGTT TGTTGTCGAC AGTGTGTCAG GGCAGTGCTG CAGACTCAGC 
GAGTTCTCAG TCACTGGATC TACGTACGCC CCTGAAGGAG AAGTTTATAA GGACGGCTCA 
CTGGTGAAGT GCAGTCAGTA TGAAGGCCTG GTGGAGATGG CCTCCATCTG TGCGCTGTGC 
AATGACTCAT CACTGGACTA CAATGAGGCA AAGGGTGTGT ATGAGAAGGT AGGGGAGGCA 
ACTGAGACTG CCCTCTGCTG TCTGGTGGAG AAAATGAACG TGTTTGACAC GGACCTGAGA 
GGACTGAGCC CTGCTGAGAG AGCTACAGCA TGCTGCTCTG TGATTAAGCA GCTCATGAGG 
AAAGAGCTGA CGCTGGAGTT TTCCAGAGAC AGGAAGTCCA TGTCTGTCTT CTGTTCATCC 
AATAAACTCA CCAGGTCTGC CTCTGGAGCC AAGATGTTCA TCAAAGGAGC TCCAGAGAGT 
GTGTTGGAGC GCTGTAATCA TATCCGGATC AGCGGTTCTG CCTGTGTGCC TTTGACCCCG 
GAGGTTCGAG AGCAGCTCCT GTCTAGAGTG CGGGAATGGG GATCAGGCCG AGACACACTG 
CGCTGCCTCG CGATGGCAAC CAGGGACACG CCCCCTGACA TTCACTGCCT CAACCTGGAG 
AACTCGGCTG CCTTTGCAAA CTATGAGACG GACCTGACCT TTGTGGGCTG TGTGGGCATG 
TTGGACCCCC CCAGGAAAGA AGTGCTCGGC GCAGTTCGAA TGTGTCGGCA GGCTGGCATA 
AGAGTCATCA TGATCACAGG TGACAACAAA GGGACGGCCC TGTCTATTTG TCGGAGGGTG 
GGCATCATCA CAGAGCAGGA AGAGGAGTTG GAGGGTGGCG GGGTAAACGG GGGTCTGACA 
GGACGAGAGT TTGATGAGCT GCCCCCGCAT CTGCAGCGTC AGGCCTGCAG GACGGTAAGG 
TGCTTCGCCC GGGTGGAACC AGCACACAAG AGCCGTATAG TGGAGTACCT GCAGAGCCTG 
AGTGACATCA CCGCCATGAC AGGTGACGGT GTGAATGATG CTCCAGCGTT AAAGAAGGCA 
GAGATTGGCA TCGCTATGGG CAGCGGCACG GCAGTGGCCA AGTCGGCCTC TGAGATGATC 
CTGGCCGATG ACAACTTCTC CACCATCGTG GCTGCAGTGG AGGAGGGCAG AGCCATTTAC 
AACAACATGA AGCAGTTCAT CAGATACCTC ATATCATCCA ACATAGGAGA GGTGGTCTGT 
ATCTTCCTGA CGGCTGCGCT GGGTATGCCT GAAGCGCTGA TCCCAGTCCA GCTGCTGTGG 
GTCAACCTGG TCACTGACGG TTTCCCAGCA ACAGCTCTGG GCTTCAACCC TCCAGACCTG 
GACATCATGT CCCGCCCTCC CCGCTCCCCC AAAGAGCCTC TGATCTCCGG CTGGTTGTTC 
TGTCGCTATC TCATCATTGG ATGTTATGTG GGAGCAGCCA CTGTGGGAGC TGCTGCCTGG 
TGGTTTATGG CAGCCCACGA TGGACCAAAA CTTTCCTTCT ATCAACTGTC CCACTACCTG 
CAGTGCAGTG AAGGCCACGC TGAGTTTGCT GGTGTGCAGT GTTCAGTCTT TGAGTCTCCT 
TATCCCATGA CTATGGCCCT GTCTGTTCTG GTTACCATAG AGATGTGCAA CGCCTTGAAC 
AGTCTTTCAG AGAACCAGTC CCTGCTGAAG ATGCCTCCCT GGTCAAACCC TTGGCTGGTG 
GGGGCGATCT GTCTGTCCAT GGCTCTACAT TTCCTCATTC TCTATGTAGA CCCACTGCCA 
GTGATCTTCC AGATCCGCCC TCTGTCGTGG CCTCAGTGGG TGGTGGTGCT GAAAATGTCC 
ATTCCTGTCA TCTTGATGGA CGAGGTTCTT AAATTTCTGG CCCGCAACTA CATCGAGCCA 
GGAAGCCAGA TCCAGTCACT GGAAGAGGAA GAGGAAGAGG AGATGCAGAG GACAAGGGGT 
AACGTGGGAT TGGTCGGTGC CGTGATGACC AGGCTGTCTC AGTCAGTGAA GGGTGTGTCC 
TGGTCATTCG TCCTGGTCTC TGCGCCGCTA GTGTTCTGGA TCTTTAGCCT GGACTCTGAC 
ATAACCAACA TCTTTTGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104937879 ( XM_010754192.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010752494.1 Larimichthys crocea sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like (LOC104937879), mRNA.

Price & Availability↑

CloneID	OLa102551
Clone ID Related Accession (Same CDS sequence)	XM_010754192.2 , XM_010754192.3
Accession Version	XM_010754192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010754192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAATG CTCATACTAA GACGGTGGAG GAGGTTTTAG GCTTCTTTGG TGTAAACGAG 
TCCACAGGTC TGAGCTGTGA GCAGCTGAAG AAGAACAGGG AGAGATGGGG ACCCAATGAA 
TTGCCAGCTG AAGAAGGGAA GTCACTTTGG GAGCTGGTCC TGGAACAGTT TGAGGATTTG 
TTGGTTCGAA TCTTGCTGCT GGCTGCATGC ATCTCCTTTA CTCTCGCTTG GTTTGAAGAA 
GGGGAAGGGA CGATCACAGC CTTCGTCGAA CCTTTCGTCA TTCTCCTCAT CCTCATTGCC 
AATGCTATTG TAGGCGTGTG GCAGGAGCGT AATGCAGAGA ATGCCATTGA GGCTCTAAAG 
GAGTATGAGC CAGAGATGGG CAAAGTGTAC CGACAAGACA AGAAGAGCGT TCAGAGGGTC 
AGAGCCAGAG ACATCGTCCC TGGAGACATC GTGGAAGTTG CTGTTGGTGA TAAGGTTCCA 
GCTGACATCA GGCTGACGTC TATCCGATCC ACCACTCTGA GGGTGGACCA GTCCATTCTG 
ACGGGGGAGT CTGTGTCAGT GCTCAAACAC ACAGATCCTG TACCAGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTCTTCTCT GGTACCAACA TTGCAGCGGG GCGAGCCATC 
GGGGTTGTTG TGGCGACAGG GGTGCAGACA GAGATTGGGA AGATCAGAGA TGAAATGGCG 
TCCACTGACC CTGAAAGGAC CCCACTACAA CAGAAACTAG ACCAGTTTGG AGAACAGCTG 
TCTAAGGTCA TCAGTGTGAT CTGTGTGGCA GTGTGGGCCA TCAATGTAGG ACACTTTAAT 
GATCCAGTCC ATGGAGGCAG CTGGCTGAGA GGAGCTGTTT ACTACTTTAA GATCGCTGTT 
GCACTGGCTG TAGCTGCCAT ACCAGAAGGG CTCCCAGCAG TCATCACTAC CTGCCTGGCT 
CTGGGCACCA GGAGGATGGC CAGAAAGAAC GCCATCGTTC GCAGCCTGCC ATCAGTGGAG 
ACGCTGGGCT GCACCTCTGT CATCTGCTCT GACAAGACGG GGACGCTGAC CACCAACCAG 
ATGTCTGTCT GCAGGATGTT TGTTGTCGAC AGTGTGTCAG GGCAGTGCTG CAGACTCAGC 
GAGTTCTCAG TCACTGGATC TACGTACGCC CCTGAAGGAG AAGTTTATAA GGACGGCTCA 
CTGGTGAAGT GCAGTCAGTA TGAAGGCCTG GTGGAGATGG CCTCCATCTG TGCGCTGTGC 
AATGACTCAT CACTGGACTA CAATGAGGCA AAGGGTGTGT ATGAGAAGGT AGGGGAGGCA 
ACTGAGACTG CCCTCTGCTG TCTGGTGGAG AAAATGAACG TGTTTGACAC GGACCTGAGA 
GGACTGAGCC CTGCTGAGAG AGCTACAGCA TGCTGCTCTG TGATTAAGCA GCTCATGAGG 
AAAGAGCTGA CGCTGGAGTT TTCCAGAGAC AGGAAGTCCA TGTCTGTCTT CTGTTCATCC 
AATAAACTCA CCAGGTCTGC CTCTGGAGCC AAGATGTTCA TCAAAGGAGC TCCAGAGAGT 
GTGTTGGAGC GCTGTAATCA TATCCGGATC AGCGGTTCTG CCTGTGTGCC TTTGACCCCG 
GAGGTTCGAG AGCAGCTCCT GTCTAGAGTG CGGGAATGGG GATCAGGCCG AGACACACTG 
CGCTGCCTCG CGATGGCAAC CAGGGACACG CCCCCTGACA TTCACTGCCT CAACCTGGAG 
AACTCGGCTG CCTTTGCAAA CTATGAGACG GACCTGACCT TTGTGGGCTG TGTGGGCATG 
TTGGACCCCC CCAGGAAAGA AGTGCTCGGC GCAGTTCGAA TGTGTCGGCA GGCTGGCATA 
AGAGTCATCA TGATCACAGG TGACAACAAA GGGACGGCCC TGTCTATTTG TCGGAGGGTG 
GGCATCATCA CAGAGCAGGA AGAGGAGTTG GAGGGTGGCG GGGTAAACGG GGGTCTGACA 
GGACGAGAGT TTGATGAGCT GCCCCCGCAT CTGCAGCGTC AGGCCTGCAG GACGGTAAGG 
TGCTTCGCCC GGGTGGAACC AGCACACAAG AGCCGTATAG TGGAGTACCT GCAGAGCCTG 
AGTGACATCA CCGCCATGAC AGGTGACGGT GTGAATGATG CTCCAGCGTT AAAGAAGGCA 
GAGATTGGCA TCGCTATGGG CAGCGGCACG GCAGTGGCCA AGTCGGCCTC TGAGATGATC 
CTGGCCGATG ACAACTTCTC CACCATCGTG GCTGCAGTGG AGGAGGGCAG AGCCATTTAC 
AACAACATGA AGCAGTTCAT CAGATACCTC ATATCATCCA ACATAGGAGA GGTGGTCTGT 
ATCTTCCTGA CGGCTGCGCT GGGTATGCCT GAAGCGCTGA TCCCAGTCCA GCTGCTGTGG 
GTCAACCTGG TCACTGACGG TTTCCCAGCA ACAGCTCTGG GCTTCAACCC TCCAGACCTG 
GACATCATGT CCCGCCCTCC CCGCTCCCCC AAAGAGCCTC TGATCTCCGG CTGGTTGTTC 
TGTCGCTATC TCATCATTGG ATGTTATGTG GGAGCAGCCA CTGTGGGAGC TGCTGCCTGG 
TGGTTTATGG CAGCCCACGA TGGACCAAAA CTTTCCTTCT ATCAACTGTC CCACTACCTG 
CAGTGCAGTG AAGGCCACGC TGAGTTTGCT GGTGTGCAGT GTTCAGTCTT TGAGTCTCCT 
TATCCCATGA CTATGGCCCT GTCTGTTCTG GTTACCATAG AGATGTGCAA CGCCTTGAAC 
AGTCTTTCAG AGAACCAGTC CCTGCTGAAG ATGCCTCCCT GGTCAAACCC TTGGCTGGTG 
GGGGCGATCT GTCTGTCCAT GGCTCTACAT TTCCTCATTC TCTATGTAGA CCCACTGCCA 
GTGATCTTCC AGATCCGCCC TCTGTCGTGG CCTCAGTGGG TGGTGGTGCT GAAAATGTCC 
ATTCCTGTCA TCTTGATGGA CGAGGTTCTT AAATTTCTGG CCCGCAACTA CATCGAGCCA 
GGAAGCCAGA TCCAGTCACT GGAAGAGGAA GAGGAAGAGG AGATGCAGAG GACAAGGGGT 
AACGTGGGAT TGGTCGGTGC CGTGATGACC AGGCTGTCTC AGTCAGTGAA GGGTGTGTCC 
TGGTCATTCG TCCTGGTCTC TGCGCCGCTA GTGTTCTGGA TCTTTAGCCT GGACTCTGAC 
ATAACCAACA TCTTTTGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010752494.1
CDS	416..3619
Translation	MDNAHTKTVEEVLGFFGVNESTGLSCEQLKKNRERWGPNELPAEEGKSLWELVLEQFEDLLVRILLLAACISFTLAWFEEGEGTITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDKKSVQRVRARDIVPGDIVEVAVGDKVPADIRLTSIRSTTLRVDQSILTGESVSVLKHTDPVPDPRAVNQDKKNMLFSGTNIAAGRAIGVVVATGVQTEIGKIRDEMASTDPERTPLQQKLDQFGEQLSKVISVICVAVWAINVGHFNDPVHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFVVDSVSGQCCRLSEFSVTGSTYAPEGEVYKDGSLVKCSQYEGLVEMASICALCNDSSLDYNEAKGVYEKVGEATETALCCLVEKMNVFDTDLRGLSPAERATACCSVIKQLMRKELTLEFSRDRKSMSVFCSSNKLTRSASGAKMFIKGAPESVLERCNHIRISGSACVPLTPEVREQLLSRVREWGSGRDTLRCLAMATRDTPPDIHCLNLENSAAFANYETDLTFVGCVGMLDPPRKEVLGAVRMCRQAGIRVIMITGDNKGTALSICRRVGIITEQEEELEGGGVNGGLTGREFDELPPHLQRQACRTVRCFARVEPAHKSRIVEYLQSLSDITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSASEMILADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNIGEVVCIFLTAALGMPEALIPVQLLWVNLVTDGFPATALGFNPPDLDIMSRPPRSPKEPLISGWLFCRYLIIGCYVGAATVGAAAWWFMAAHDGPKLSFYQLSHYLQCSEGHAEFAGVQCSVFESPYPMTMALSVLVTIEMCNALNSLSENQSLLKMPPWSNPWLVGAICLSMALHFLILYVDPLPVIFQIRPLSWPQWVVVLKMSIPVILMDEVLKFLARNYIEPGSQIQSLEEEEEEEMQRTRGNVGLVGAVMTRLSQSVKGVSWSFVLVSAPLVFWIFSLDSDITNIFWE

Target ORF information:

RefSeq Version	XM_010754192.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea sarcoplasmic/endoplasmic reticulum calcium ATPase 2-like (LOC104937879), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010754192.3

ORF Insert Sequence:

ATGGATAATG CTCATACTAA GACGGTGGAG GAGGTTTTAG GCTTCTTTGG TGTAAACGAG 
TCCACAGGTC TGAGCTGTGA GCAGCTGAAG AAGAACAGGG AGAGATGGGG ACCCAATGAA 
TTGCCAGCTG AAGAAGGGAA GTCACTTTGG GAGCTGGTCC TGGAACAGTT TGAGGATTTG 
TTGGTTCGAA TCTTGCTGCT GGCTGCATGC ATCTCCTTTA CTCTCGCTTG GTTTGAAGAA 
GGGGAAGGGA CGATCACAGC CTTCGTCGAA CCTTTCGTCA TTCTCCTCAT CCTCATTGCC 
AATGCTATTG TAGGCGTGTG GCAGGAGCGT AATGCAGAGA ATGCCATTGA GGCTCTAAAG 
GAGTATGAGC CAGAGATGGG CAAAGTGTAC CGACAAGACA AGAAGAGCGT TCAGAGGGTC 
AGAGCCAGAG ACATCGTCCC TGGAGACATC GTGGAAGTTG CTGTTGGTGA TAAGGTTCCA 
GCTGACATCA GGCTGACGTC TATCCGATCC ACCACTCTGA GGGTGGACCA GTCCATTCTG 
ACGGGGGAGT CTGTGTCAGT GCTCAAACAC ACAGATCCTG TACCAGACCC CCGAGCTGTC 
AACCAGGACA AGAAGAACAT GCTCTTCTCT GGTACCAACA TTGCAGCGGG GCGAGCCATC 
GGGGTTGTTG TGGCGACAGG GGTGCAGACA GAGATTGGGA AGATCAGAGA TGAAATGGCG 
TCCACTGACC CTGAAAGGAC CCCACTACAA CAGAAACTAG ACCAGTTTGG AGAACAGCTG 
TCTAAGGTCA TCAGTGTGAT CTGTGTGGCA GTGTGGGCCA TCAATGTAGG ACACTTTAAT 
GATCCAGTCC ATGGAGGCAG CTGGCTGAGA GGAGCTGTTT ACTACTTTAA GATCGCTGTT 
GCACTGGCTG TAGCTGCCAT ACCAGAAGGG CTCCCAGCAG TCATCACTAC CTGCCTGGCT 
CTGGGCACCA GGAGGATGGC CAGAAAGAAC GCCATCGTTC GCAGCCTGCC ATCAGTGGAG 
ACGCTGGGCT GCACCTCTGT CATCTGCTCT GACAAGACGG GGACGCTGAC CACCAACCAG 
ATGTCTGTCT GCAGGATGTT TGTTGTCGAC AGTGTGTCAG GGCAGTGCTG CAGACTCAGC 
GAGTTCTCAG TCACTGGATC TACGTACGCC CCTGAAGGAG AAGTTTATAA GGACGGCTCA 
CTGGTGAAGT GCAGTCAGTA TGAAGGCCTG GTGGAGATGG CCTCCATCTG TGCGCTGTGC 
AATGACTCAT CACTGGACTA CAATGAGGCA AAGGGTGTGT ATGAGAAGGT AGGGGAGGCA 
ACTGAGACTG CCCTCTGCTG TCTGGTGGAG AAAATGAACG TGTTTGACAC GGACCTGAGA 
GGACTGAGCC CTGCTGAGAG AGCTACAGCA TGCTGCTCTG TGATTAAGCA GCTCATGAGG 
AAAGAGCTGA CGCTGGAGTT TTCCAGAGAC AGGAAGTCCA TGTCTGTCTT CTGTTCATCC 
AATAAACTCA CCAGGTCTGC CTCTGGAGCC AAGATGTTCA TCAAAGGAGC TCCAGAGAGT 
GTGTTGGAGC GCTGTAATCA TATCCGGATC AGCGGTTCTG CCTGTGTGCC TTTGACCCCG 
GAGGTTCGAG AGCAGCTCCT GTCTAGAGTG CGGGAATGGG GATCAGGCCG AGACACACTG 
CGCTGCCTCG CGATGGCAAC CAGGGACACG CCCCCTGACA TTCACTGCCT CAACCTGGAG 
AACTCGGCTG CCTTTGCAAA CTATGAGACG GACCTGACCT TTGTGGGCTG TGTGGGCATG 
TTGGACCCCC CCAGGAAAGA AGTGCTCGGC GCAGTTCGAA TGTGTCGGCA GGCTGGCATA 
AGAGTCATCA TGATCACAGG TGACAACAAA GGGACGGCCC TGTCTATTTG TCGGAGGGTG 
GGCATCATCA CAGAGCAGGA AGAGGAGTTG GAGGGTGGCG GGGTAAACGG GGGTCTGACA 
GGACGAGAGT TTGATGAGCT GCCCCCGCAT CTGCAGCGTC AGGCCTGCAG GACGGTAAGG 
TGCTTCGCCC GGGTGGAACC AGCACACAAG AGCCGTATAG TGGAGTACCT GCAGAGCCTG 
AGTGACATCA CCGCCATGAC AGGTGACGGT GTGAATGATG CTCCAGCGTT AAAGAAGGCA 
GAGATTGGCA TCGCTATGGG CAGCGGCACG GCAGTGGCCA AGTCGGCCTC TGAGATGATC 
CTGGCCGATG ACAACTTCTC CACCATCGTG GCTGCAGTGG AGGAGGGCAG AGCCATTTAC 
AACAACATGA AGCAGTTCAT CAGATACCTC ATATCATCCA ACATAGGAGA GGTGGTCTGT 
ATCTTCCTGA CGGCTGCGCT GGGTATGCCT GAAGCGCTGA TCCCAGTCCA GCTGCTGTGG 
GTCAACCTGG TCACTGACGG TTTCCCAGCA ACAGCTCTGG GCTTCAACCC TCCAGACCTG 
GACATCATGT CCCGCCCTCC CCGCTCCCCC AAAGAGCCTC TGATCTCCGG CTGGTTGTTC 
TGTCGCTATC TCATCATTGG ATGTTATGTG GGAGCAGCCA CTGTGGGAGC TGCTGCCTGG 
TGGTTTATGG CAGCCCACGA TGGACCAAAA CTTTCCTTCT ATCAACTGTC CCACTACCTG 
CAGTGCAGTG AAGGCCACGC TGAGTTTGCT GGTGTGCAGT GTTCAGTCTT TGAGTCTCCT 
TATCCCATGA CTATGGCCCT GTCTGTTCTG GTTACCATAG AGATGTGCAA CGCCTTGAAC 
AGTCTTTCAG AGAACCAGTC CCTGCTGAAG ATGCCTCCCT GGTCAAACCC TTGGCTGGTG 
GGGGCGATCT GTCTGTCCAT GGCTCTACAT TTCCTCATTC TCTATGTAGA CCCACTGCCA 
GTGATCTTCC AGATCCGCCC TCTGTCGTGG CCTCAGTGGG TGGTGGTGCT GAAAATGTCC 
ATTCCTGTCA TCTTGATGGA CGAGGTTCTT AAATTTCTGG CCCGCAACTA CATCGAGCCA 
GGAAGCCAGA TCCAGTCACT GGAAGAGGAA GAGGAAGAGG AGATGCAGAG GACAAGGGGT 
AACGTGGGAT TGGTCGGTGC CGTGATGACC AGGCTGTCTC AGTCAGTGAA GGGTGTGTCC 
TGGTCATTCG TCCTGGTCTC TGCGCCGCTA GTGTTCTGGA TCTTTAGCCT GGACTCTGAC 
ATAACCAACA TCTTTTGGGA GTGA