LOC104934663 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104934663
Entrez Gene ID	104934663
Full Name	ASH1 like histone lysine methyltransferase
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010750379.2	XP_010748681.2	histone-lysine N-methyltransferase ASH1L isoform X1
XM_010750380.2	XP_010748682.2	histone-lysine N-methyltransferase ASH1L isoform X2
XM_010750379.3	XP_010748681.2	histone-lysine N-methyltransferase ASH1L isoform X1
XM_027286255.1	XP_027142056.1	histone-lysine N-methyltransferase ASH1L isoform X2
XM_010750381.3	XP_010748683.2	histone-lysine N-methyltransferase ASH1L isoform X3
XM_010750381.2	XP_010748683.2	histone-lysine N-methyltransferase ASH1L isoform X3
XM_027286256.1	XP_027142057.1	histone-lysine N-methyltransferase ASH1L isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC104934663 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104934663 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa115686	XM_010750380.2 Latest version!	Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa115687	XM_010750381.2 Latest version!	Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa203015	XM_027286255.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa203015	XM_027286256.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa203016	XM_010750381.3 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa115685	XM_010750379.2 Latest version!	Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OLa203014	XM_010750379.3 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104934663 ( XM_010750380.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010748682.2 Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLa115686
Clone ID Related Accession (Same CDS sequence)	XM_010750380.2
Accession Version	XM_010750380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase ASH1L isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609812.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010750380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACG 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCA 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCGGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTGGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AAAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTGAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGT 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GGTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010748682.2
CDS	1088..6976
Translation	MDQRVKGGTPPTTNSTLEPTSAPEDSEQDQVAEKKWRGEGENRDVGEKEEEKKGAGRKREGDKGAVLELLIEGRCGSAGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGAPLRGDGEGGVGQQGKTSSAPDEATRDQRSGLDSVQTTSKQTASAASPPLASSSSLSLPSSSSSSSQPLSLLSTFTAASTPASVPAITSQGTKSAKQVPIKLADKTEVNSNGSSERVKKEKLPSVNGQPVLAGHKPCSSTTDQTASTTPSNTCIQALSASETRNEGKTSKKHNGLVQTKQKGLSNGKGSTDILSTSTSSKIKAGKHSSSSSVSSADQSPRPPVSTSTHKELSLSNKSRPDSPSSPSVTPKPQSSPNVDLSSAQSQDQDSSPQASLEKKKEKERKAKKEKRKDKKSKRDRLEAERAKSEGRKEEGKKKKKEKGKDGKSRHGKEKDERHKTDDTWRDELKVERGKGEKKRDKVSPDRQRTEDNNAKCGETVDSGKLDKTSKVVNPGRPEEIDKADDSCKPVKLSEPATGDGSKSKEQDVSRHSTVPPSHPSSSAPPSVPTPSARAPVSPPSSPQEQDSRPLKKRKARRPSWTKLVHRAQRAENQEAPSDSQHNSLLGFPQNPKTSLPAKATIQQTDESHPAPSTSLTSSSSPFSSATKPPSPKQSHPTSDSSPPASRCPITPARKRGRPKSHSSSLDEPPPRLSPNAIPTEVPLLGCDGIQKAPVLEPSPILQCAAQSKSSPKKRGRPPKRPLPEDQSGDALNRTDDADRSKDFHPPEKGNRQLKIRRLINEMKKRKKRRLQKVMLSGYVGKEGKGSEAADGETSLRMCKSIEATTVHTLSALSSSFGSKLGPQINVSKRGTIYMGKRRGRKPKTQTAPTVQTNSQNSTQSSLFSNPSETSLFSNQPQPPPSHPFPSPSLTHSSGAQSPYSEGSLAEPTSSLIFPHPFSLPSPSSSCTSPRPPSSSSLSPFVKKSCPCQGRHHFPFHQSSCKLSCPTPPLHHTPGSPGHLKEATPSPRSESHSEETLPSDSGIGTDNNSVSERGEIRGARGMLRLGQGSGVILGGQRHPSSLVDRPSPVSSPLSHMPRHTNPITNSSTVERHRERDRHRHRRRDFDCSSSCTCLCPCSCPGHNKCTHSDYYSCLGHNALKRQKNKHKKKHQQLHMQDPEFLAELEDLIGQFSEVHIGRRGWARTGLGQGFDGSGNAAGGRRHHSSSHSLRSNIFRINLNGFYSPHPSPYSANTSFSPQPFYPCQPVHCNRKPDRRQCACPSKFQETIENMGFYSSYPPATTLYHHLPSSYPLPSPHQYGPHQSHHAHFLLNPARFHRRRSRLLREGALGGEVEGDVGGGSGGGPHLSSGFTSSLSCGCGRNEHKHKHKHRHRHCERDLDDEEELHEDEEEDGMEREGLASSKPRSGFILGQGEGGRKGARGMGSMLTKESPWLCENGNDSFSSAAAVTSSASAERYKHTSLTSLGLGSSHLSSFGGSWGALGQSWTKFGGLGGTGFGNSNPSWRGFSGEQHTGRLIASDGEDDDSEDIQEPHLYRASPSPTHTNLFTSAAMATGGRGLRSGLAGKNPGSGDRSWRRDEPAWTERRETGLQGDSRSRGQQKSVPTPDSVAGKNKRRPGRPRKHPLPSTIPSPTHSSAAPSMSSPDLLPGHSHNRDGREVGGRKDERGPERDRGADTVQQVTELELQAKRKRGRKRKHGDSPCHQSVTEDKPECDTPPECFSQSDVDQAPSQPVVIQREERADGPPRKKFLRAGLYSDDYKTTDPPSQAQQLCRESMEYTPEEHEYSLLPAPIHVGKYLRLKRIHFQLPYDVMWLWQHNQLQRQPAVPMKRKRRYCRLKERTLSSHQTAEENSSDITSLFPHLDMEPLTSSERSFVVKHHVFLVRNWELVRDRQIRMRIERERERDAEAEEEDSRRLSFDRANGDDSHIKSGP

Target ORF information:

RefSeq Version	XM_010750380.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010750380.2

ORF Insert Sequence:

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACG 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCA 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCGGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTGGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AAAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTGAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGT 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GGTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104934663 ( XM_010750381.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010748683.2 Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X3, mRNA.

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CloneID	OLa115687
Clone ID Related Accession (Same CDS sequence)	XM_010750381.2
Accession Version	XM_010750381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase ASH1L isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609812.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010750381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACG 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCA 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCGGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTGGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AAAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTGAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGT 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGCACTGT GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010748683.2
CDS	1089..6305
Translation	MDQRVKGGTPPTTNSTLEPTSAPEDSEQDQVAEKKWRGEGENRDVGEKEEEKKGAGRKREGDKGAVLELLIEGRCGSAGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGAPLRGDGEGGVGQQGKTSSAPDEATRDQRSGLDSVQTTSKQTASAASPPLASSSSLSLPSSSSSSSQPLSLLSTFTAASTPASVPAITSQGTKSAKQVPIKLADKTEVNSNGSSERVKKEKLPSVNGQPVLAGHKPCSSTTDQTASTTPSNTCIQALSASETRNEGKTSKKHNGLVQTKQKGLSNGKGSTDILSTSTSSKIKAGKHSSSSSVSSADQSPRPPVSTSTHKELSLSNKSRPDSPSSPSVTPKPQSSPNVDLSSAQSQDQDSSPQASLEKKKEKERKAKKEKRKDKKSKRDRLEAERAKSEGRKEEGKKKKKEKGKDGKSRHGKEKDERHKTDDTWRDELKVERGKGEKKRDKVSPDRQRTEDNNAKCGETVDSGKLDKTSKVVNPGRPEEIDKADDSCKPVKLSEPATGDGSKSKEQDVSRHSTVPPSHPSSSAPPSVPTPSARAPVSPPSSPQEQDSRPLKKRKARRPSWTKLVHRAQRAENQEAPSDSQHNSLLGFPQNPKTSLPAKATIQQTDESHPAPSTSLTSSSSPFSSATKPPSPKQSHPTSDSSPPASRCPITPARKRGRPKSHSSSLDEPPPRLSPNAIPTEVPLLGCDGIQKAPVLEPSPILQCAAQSKSSPKKRGRPPKRPLPEDQSGDALNRTDDADRSKDFHPPEKGNRQLKIRRLINEMKKRKKRRLQKVMLSGYVGKEGKGSEAADGETSLRMCKSIEATTVHTLSALSSSFGSKLGPQINVSKRGTIYMGKRRGRKPKTQTAPTVQTNSQNSTQSSLFSNPSETSLFSNQPQPPPSHPFPSPSLTHSSGAQSPYSEGSLAEPTSSLIFPHPFSLPSPSSSCTSPRPPSSSSLSPFVKKSCPCQGRHHFPFHQSSCKLSCPTPPLHHTPGSPGHLKEATPSPRSESHSEETLPSDSGIGTDNNSVSERGEIRGARGMLRLGQGSGVILGGQRHPSSLVDRPSPVSSPLSHMPRHTNPITNSSTVERHRERDRHRHRRRDFDCSSSCTCLCPCSCPGHNKCTHSDYYSCLGHNALKRQKNKHKKKHQQLHMQDPEFLAELEDLIGQFSEVHIGRRGWARTGLGQGFDGSGNAAGGRRHHSSSHSLRSNIFRINLNGFYSPHPSPYSANTSFSPQPFYPCQPVHCNRKPDRRQCACPSKFQETIENMGFYSSYPPATTLYHHLPSSYPLPSPHQYGPHQSHHAHFLLNPARFHRRRSRLLREGALGGEVEGDVGGGSGGGPHLSSGFTSSLSCGCGRNEHKHKHKHRHRHCERDLDDEEELHEDEEEDGMEREGLASSKPRSGFILGQGEGGRKGARGMGSMLTKESPWLCENGNDSFSSAAAVTSSASAERYKHTSLTSLGLGSSHLSSFGGSWGALGQSWTKFGGLGGTGFGNSNPSWRGFSGEQHTGRLIASDGEDDDSEDIQEPHLYRASPSPTHTNLFTSAAMATGGRGLRSGLAGKNPGSGDRSWRRDEPAWTERRETGLQGDSRSRGQQKSVPTPDSVAGKNKRRPGRPRKHPLPSTIPSPTHSSAAPSMSSPDLLPGHSHNRDGREVGGRKDERGPERDRGADTVQQVTELELQAKRKRGRKRKHGDSPCHQSTVC

Target ORF information:

RefSeq Version	XM_010750381.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010750381.2

ORF Insert Sequence:

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACG 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCA 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCGGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTGGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AAAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTGAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGT 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGCACTGT GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104934663 ( XM_027286255.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027142056.1 Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X3, mRNA.

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CloneID	OLa203015
Clone ID Related Accession (Same CDS sequence)	XM_027286255.1 , XM_027286256.1
Accession Version	XM_027286255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase ASH1L isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040023.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GGTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027142056.1
CDS	1114..7002
Translation	MDQRVKGGTPPTTNSTLEPTSAPEDSEQDQVAEKKWRGEGENRDVGEKEEEKKGAGRKREGDKGAVLELLIEGRCGSAGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGAPLRGDGEGGVGQQGKTSSAPDEATRDQRSGLDSVQTTSKQTASAASPPLASSSSLSLPSSSSSSSQPLSLLSTFTAASTPASVPAITSQGTKSAKQVPIKLADKTEVNSNGSSERVKKEKLPSVNGQPVLAGHKPCSSTTDQTASTTPSNTCIQALSASETRNEGKTSKKHNGLVQTKQKGLSNGKGSTDILSTSTSSKIKAGKHSSSSSVSSADQSPRPPVSTSTHKELSLSNKSRPDSPSSPSVTPKPQSSPNVDLSSAQSQDQDSSPQASLEKKKEKERKAKKEKRKDKKSKRDRLEAERAKSEGRKEEGKKKKKEKGKDGKSRHGKEKDERHKTDDTWRDELKVERGKGEKKRDKVSPDRQRTEDNNAKCGETVDSGKLDKTSKVVNPGRPEEIDKADDSCKPVKLSEPATGDGSKSKEQDVSRHSTVPPSHPSSSAPPSVPTPSARAPVSPPSSPQEQDSRPLKKRKARRPSWTKLVHRAQRAENQEAPSDSQHNSLLGFPQNPKTSLPAKATIQQTDESHPAPSTSLTSSSSPFSSATKPPSPKQSHPTSDSSPPASRCPITPARKRGRPKSHSSSLDEPPPRLSPNAIPTEVPLLGCDGIQKAPVLEPSPILQCAAQSKSSPKKRGRPPKRPLPEDQSGDALNRTDDADRSKDFHPPEKGNRQLKIRRLINEMKKRKKRRLQKVMLSGYVGKEGKGSEAADGETSLRMCKSIEATTVHTLSALSSSFGSKLGPQINVSKRGTIYMGKRRGRKPKTQTAPTVQTNSQNSTQSSLFSNPSETSLFSNQPQPPPSHPFPSPSLTHSSGAQSPYSEGSLAEPTSSLIFPHPFSLPSPSSSCTSPRPPSSSSLSPFVKKSCPCQGRHHFPFHQSSCKLSCPTPPLHHTPGSPGHLKEATPSPRSESHSEETLPSDSGIGTDNNSVSERGEIRGARGMLRLGQGSGVILGGQRHPSSLVDRPSPVSSPLSHMPRHTNPITNSSTVERHRERDRHRHRRRDFDCSSSCTCLCPCSCPGHNKCTHSDYYSCLGHNALKRQKNKHKKKHQQLHMQDPEFLAELEDLIGQFSEVHIGRRGWARTGLGQGFDGSGNAAGGRRHHSSSHSLRSNIFRINLNGFYSPHPSPYSANTSFSPQPFYPCQPVHCNRKPDRRQCACPSKFQETIENMGFYSSYPPATTLYHHLPSSYPLPSPHQYGPHQSHHAHFLLNPARFHRRRSRLLREGALGGEVEGDVGGGSGGGPHLSSGFTSSLSCGCGRNEHKHKHKHRHRHCERDLDDEEELHEDEEEDGMEREGLASSKPRSGFILGQGEGGRKGARGMGSMLTKESPWLCENGNDSFSSAAAVTSSASAERYKHTSLTSLGLGSSHLSSFGGSWGALGQSWTKFGGLGGTGFGNSNPSWRGFSGEQHTGRLIASDGEDDDSEDIQEPHLYRASPSPTHTNLFTSAAMATGGRGLRSGLAGKNPGSGDRSWRRDEPAWTERRETGLQGDSRSRGQQKSVPTPDSVAGKNKRRPGRPRKHPLPSTIPSPTHSSAAPSMSSPDLLPGHSHNRDGREVGGRKDERGPERDRGADTVQQVTELELQAKRKRGRKRKHGDSPCHQSVTEDKPECDTPPECFSQSDVDQAPSQPVVIQREERADGPPRKKFLRAGLYSDDYKTTDPPSQAQQLCRESMEYTPEEHEYSLLPAPIHVGKYLRLKRIHFQLPYDVMWLWQHNQLQRQPAVPMKRKRRYCRLKERTLSSHQTAEENSSDITSLFPHLDMEPLTSSERSFVVKHHVFLVRNWELVRDRQIRMRIERERERDAEAEEEDSRRLSFDRANGDDSHIKSGP

Target ORF information:

RefSeq Version	XM_027286255.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027286255.1

ORF Insert Sequence:

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GGTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104934663 ( XM_027286256.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027142057.1 Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X4, mRNA.

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CloneID	OLa203015
Clone ID Related Accession (Same CDS sequence)	XM_027286255.1 , XM_027286256.1
Accession Version	XM_027286256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase ASH1L isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040023.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GGTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027142057.1
CDS	1114..7002
Translation	MDQRVKGGTPPTTNSTLEPTSAPEDSEQDQVAEKKWRGEGENRDVGEKEEEKKGAGRKREGDKGAVLELLIEGRCGSAGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGAPLRGDGEGGVGQQGKTSSAPDEATRDQRSGLDSVQTTSKQTASAASPPLASSSSLSLPSSSSSSSQPLSLLSTFTAASTPASVPAITSQGTKSAKQVPIKLADKTEVNSNGSSERVKKEKLPSVNGQPVLAGHKPCSSTTDQTASTTPSNTCIQALSASETRNEGKTSKKHNGLVQTKQKGLSNGKGSTDILSTSTSSKIKAGKHSSSSSVSSADQSPRPPVSTSTHKELSLSNKSRPDSPSSPSVTPKPQSSPNVDLSSAQSQDQDSSPQASLEKKKEKERKAKKEKRKDKKSKRDRLEAERAKSEGRKEEGKKKKKEKGKDGKSRHGKEKDERHKTDDTWRDELKVERGKGEKKRDKVSPDRQRTEDNNAKCGETVDSGKLDKTSKVVNPGRPEEIDKADDSCKPVKLSEPATGDGSKSKEQDVSRHSTVPPSHPSSSAPPSVPTPSARAPVSPPSSPQEQDSRPLKKRKARRPSWTKLVHRAQRAENQEAPSDSQHNSLLGFPQNPKTSLPAKATIQQTDESHPAPSTSLTSSSSPFSSATKPPSPKQSHPTSDSSPPASRCPITPARKRGRPKSHSSSLDEPPPRLSPNAIPTEVPLLGCDGIQKAPVLEPSPILQCAAQSKSSPKKRGRPPKRPLPEDQSGDALNRTDDADRSKDFHPPEKGNRQLKIRRLINEMKKRKKRRLQKVMLSGYVGKEGKGSEAADGETSLRMCKSIEATTVHTLSALSSSFGSKLGPQINVSKRGTIYMGKRRGRKPKTQTAPTVQTNSQNSTQSSLFSNPSETSLFSNQPQPPPSHPFPSPSLTHSSGAQSPYSEGSLAEPTSSLIFPHPFSLPSPSSSCTSPRPPSSSSLSPFVKKSCPCQGRHHFPFHQSSCKLSCPTPPLHHTPGSPGHLKEATPSPRSESHSEETLPSDSGIGTDNNSVSERGEIRGARGMLRLGQGSGVILGGQRHPSSLVDRPSPVSSPLSHMPRHTNPITNSSTVERHRERDRHRHRRRDFDCSSSCTCLCPCSCPGHNKCTHSDYYSCLGHNALKRQKNKHKKKHQQLHMQDPEFLAELEDLIGQFSEVHIGRRGWARTGLGQGFDGSGNAAGGRRHHSSSHSLRSNIFRINLNGFYSPHPSPYSANTSFSPQPFYPCQPVHCNRKPDRRQCACPSKFQETIENMGFYSSYPPATTLYHHLPSSYPLPSPHQYGPHQSHHAHFLLNPARFHRRRSRLLREGALGGEVEGDVGGGSGGGPHLSSGFTSSLSCGCGRNEHKHKHKHRHRHCERDLDDEEELHEDEEEDGMEREGLASSKPRSGFILGQGEGGRKGARGMGSMLTKESPWLCENGNDSFSSAAAVTSSASAERYKHTSLTSLGLGSSHLSSFGGSWGALGQSWTKFGGLGGTGFGNSNPSWRGFSGEQHTGRLIASDGEDDDSEDIQEPHLYRASPSPTHTNLFTSAAMATGGRGLRSGLAGKNPGSGDRSWRRDEPAWTERRETGLQGDSRSRGQQKSVPTPDSVAGKNKRRPGRPRKHPLPSTIPSPTHSSAAPSMSSPDLLPGHSHNRDGREVGGRKDERGPERDRGADTVQQVTELELQAKRKRGRKRKHGDSPCHQSVTEDKPECDTPPECFSQSDVDQAPSQPVVIQREERADGPPRKKFLRAGLYSDDYKTTDPPSQAQQLCRESMEYTPEEHEYSLLPAPIHVGKYLRLKRIHFQLPYDVMWLWQHNQLQRQPAVPMKRKRRYCRLKERTLSSHQTAEENSSDITSLFPHLDMEPLTSSERSFVVKHHVFLVRNWELVRDRQIRMRIERERERDAEAEEEDSRRLSFDRANGDDSHIKSGP

Target ORF information:

RefSeq Version	XM_027286256.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027286256.1

ORF Insert Sequence:

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GGTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104934663 ( XM_010750381.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010748683.2 Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X5, mRNA.

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CloneID	OLa203016
Clone ID Related Accession (Same CDS sequence)	XM_010750381.3
Accession Version	XM_010750381.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase ASH1L isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040023.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010750381.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGCACTGT GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010748683.2
CDS	1117..6333
Translation	MDQRVKGGTPPTTNSTLEPTSAPEDSEQDQVAEKKWRGEGENRDVGEKEEEKKGAGRKREGDKGAVLELLIEGRCGSAGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGAPLRGDGEGGVGQQGKTSSAPDEATRDQRSGLDSVQTTSKQTASAASPPLASSSSLSLPSSSSSSSQPLSLLSTFTAASTPASVPAITSQGTKSAKQVPIKLADKTEVNSNGSSERVKKEKLPSVNGQPVLAGHKPCSSTTDQTASTTPSNTCIQALSASETRNEGKTSKKHNGLVQTKQKGLSNGKGSTDILSTSTSSKIKAGKHSSSSSVSSADQSPRPPVSTSTHKELSLSNKSRPDSPSSPSVTPKPQSSPNVDLSSAQSQDQDSSPQASLEKKKEKERKAKKEKRKDKKSKRDRLEAERAKSEGRKEEGKKKKKEKGKDGKSRHGKEKDERHKTDDTWRDELKVERGKGEKKRDKVSPDRQRTEDNNAKCGETVDSGKLDKTSKVVNPGRPEEIDKADDSCKPVKLSEPATGDGSKSKEQDVSRHSTVPPSHPSSSAPPSVPTPSARAPVSPPSSPQEQDSRPLKKRKARRPSWTKLVHRAQRAENQEAPSDSQHNSLLGFPQNPKTSLPAKATIQQTDESHPAPSTSLTSSSSPFSSATKPPSPKQSHPTSDSSPPASRCPITPARKRGRPKSHSSSLDEPPPRLSPNAIPTEVPLLGCDGIQKAPVLEPSPILQCAAQSKSSPKKRGRPPKRPLPEDQSGDALNRTDDADRSKDFHPPEKGNRQLKIRRLINEMKKRKKRRLQKVMLSGYVGKEGKGSEAADGETSLRMCKSIEATTVHTLSALSSSFGSKLGPQINVSKRGTIYMGKRRGRKPKTQTAPTVQTNSQNSTQSSLFSNPSETSLFSNQPQPPPSHPFPSPSLTHSSGAQSPYSEGSLAEPTSSLIFPHPFSLPSPSSSCTSPRPPSSSSLSPFVKKSCPCQGRHHFPFHQSSCKLSCPTPPLHHTPGSPGHLKEATPSPRSESHSEETLPSDSGIGTDNNSVSERGEIRGARGMLRLGQGSGVILGGQRHPSSLVDRPSPVSSPLSHMPRHTNPITNSSTVERHRERDRHRHRRRDFDCSSSCTCLCPCSCPGHNKCTHSDYYSCLGHNALKRQKNKHKKKHQQLHMQDPEFLAELEDLIGQFSEVHIGRRGWARTGLGQGFDGSGNAAGGRRHHSSSHSLRSNIFRINLNGFYSPHPSPYSANTSFSPQPFYPCQPVHCNRKPDRRQCACPSKFQETIENMGFYSSYPPATTLYHHLPSSYPLPSPHQYGPHQSHHAHFLLNPARFHRRRSRLLREGALGGEVEGDVGGGSGGGPHLSSGFTSSLSCGCGRNEHKHKHKHRHRHCERDLDDEEELHEDEEEDGMEREGLASSKPRSGFILGQGEGGRKGARGMGSMLTKESPWLCENGNDSFSSAAAVTSSASAERYKHTSLTSLGLGSSHLSSFGGSWGALGQSWTKFGGLGGTGFGNSNPSWRGFSGEQHTGRLIASDGEDDDSEDIQEPHLYRASPSPTHTNLFTSAAMATGGRGLRSGLAGKNPGSGDRSWRRDEPAWTERRETGLQGDSRSRGQQKSVPTPDSVAGKNKRRPGRPRKHPLPSTIPSPTHSSAAPSMSSPDLLPGHSHNRDGREVGGRKDERGPERDRGADTVQQVTELELQAKRKRGRKRKHGDSPCHQSTVC

Target ORF information:

RefSeq Version	XM_010750381.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010750381.3

ORF Insert Sequence:

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGCACTGT GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104934663 ( XM_010750379.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010748681.2 Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X1, mRNA.

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CloneID	OLa115685
Clone ID Related Accession (Same CDS sequence)	XM_010750379.2
Accession Version	XM_010750379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609812.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010750379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACG 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCA 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCGGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTGGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AAAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTGAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGT 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GATCAGCCAG TGGGGGTGGA GGTGACGGTT ATCAGCAGTG ACCCCCACCA TCAGAGTCAG 
GACACCTCCA GCTTGCTCAC TGCCAGCCCC TGTCCCACCA AAAACCAGAA TAGACAAGAG 
GAGGAGGGAG AGGAAGAAAA AAGAGGGGAG GAAGAGGTCT GCAGCAGAGA CCAGAGGAGG 
AAACGGCTGA ATGATTTACT TTTGACCCTG CAGCATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010748681.2
CDS	1087..7188
Translation	MDQRVKGGTPPTTNSTLEPTSAPEDSEQDQVAEKKWRGEGENRDVGEKEEEKKGAGRKREGDKGAVLELLIEGRCGSAGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGAPLRGDGEGGVGQQGKTSSAPDEATRDQRSGLDSVQTTSKQTASAASPPLASSSSLSLPSSSSSSSQPLSLLSTFTAASTPASVPAITSQGTKSAKQVPIKLADKTEVNSNGSSERVKKEKLPSVNGQPVLAGHKPCSSTTDQTASTTPSNTCIQALSASETRNEGKTSKKHNGLVQTKQKGLSNGKGSTDILSTSTSSKIKAGKHSSSSSVSSADQSPRPPVSTSTHKELSLSNKSRPDSPSSPSVTPKPQSSPNVDLSSAQSQDQDSSPQASLEKKKEKERKAKKEKRKDKKSKRDRLEAERAKSEGRKEEGKKKKKEKGKDGKSRHGKEKDERHKTDDTWRDELKVERGKGEKKRDKVSPDRQRTEDNNAKCGETVDSGKLDKTSKVVNPGRPEEIDKADDSCKPVKLSEPATGDGSKSKEQDVSRHSTVPPSHPSSSAPPSVPTPSARAPVSPPSSPQEQDSRPLKKRKARRPSWTKLVHRAQRAENQEAPSDSQHNSLLGFPQNPKTSLPAKATIQQTDESHPAPSTSLTSSSSPFSSATKPPSPKQSHPTSDSSPPASRCPITPARKRGRPKSHSSSLDEPPPRLSPNAIPTEVPLLGCDGIQKAPVLEPSPILQCAAQSKSSPKKRGRPPKRPLPEDQSGDALNRTDDADRSKDFHPPEKGNRQLKIRRLINEMKKRKKRRLQKVMLSGYVGKEGKGSEAADGETSLRMCKSIEATTVHTLSALSSSFGSKLGPQINVSKRGTIYMGKRRGRKPKTQTAPTVQTNSQNSTQSSLFSNPSETSLFSNQPQPPPSHPFPSPSLTHSSGAQSPYSEGSLAEPTSSLIFPHPFSLPSPSSSCTSPRPPSSSSLSPFVKKSCPCQGRHHFPFHQSSCKLSCPTPPLHHTPGSPGHLKEATPSPRSESHSEETLPSDSGIGTDNNSVSERGEIRGARGMLRLGQGSGVILGGQRHPSSLVDRPSPVSSPLSHMPRHTNPITNSSTVERHRERDRHRHRRRDFDCSSSCTCLCPCSCPGHNKCTHSDYYSCLGHNALKRQKNKHKKKHQQLHMQDPEFLAELEDLIGQFSEVHIGRRGWARTGLGQGFDGSGNAAGGRRHHSSSHSLRSNIFRINLNGFYSPHPSPYSANTSFSPQPFYPCQPVHCNRKPDRRQCACPSKFQETIENMGFYSSYPPATTLYHHLPSSYPLPSPHQYGPHQSHHAHFLLNPARFHRRRSRLLREGALGGEVEGDVGGGSGGGPHLSSGFTSSLSCGCGRNEHKHKHKHRHRHCERDLDDEEELHEDEEEDGMEREGLASSKPRSGFILGQGEGGRKGARGMGSMLTKESPWLCENGNDSFSSAAAVTSSASAERYKHTSLTSLGLGSSHLSSFGGSWGALGQSWTKFGGLGGTGFGNSNPSWRGFSGEQHTGRLIASDGEDDDSEDIQEPHLYRASPSPTHTNLFTSAAMATGGRGLRSGLAGKNPGSGDRSWRRDEPAWTERRETGLQGDSRSRGQQKSVPTPDSVAGKNKRRPGRPRKHPLPSTIPSPTHSSAAPSMSSPDLLPGHSHNRDGREVGGRKDERGPERDRGADTVQQVTELELQAKRKRGRKRKHGDSPCHQSVTEDKPECDTPPECFSQSDVDQAPSQPVVIQREERADGPPRKKFLRAGLYSDDYKTTDPPSQAQQLCRESMEYTPEEHEYSLLPAPIHVGKYLRLKRIHFQLPYDVMWLWQHNQLQRQPAVPMKRKRRYCRLKERTLSSHQTAEENSSDITSLFPHLDMEPLTSSERSFVVKHHVFLVRNWELVRDRQIRMRIERERERDAEAEEEDSRRLSFDRANGDDSHIKSDQPVGVEVTVISSDPHHQSQDTSSLLTASPCPTKNQNRQEEEGEEEKRGEEEVCSRDQRRKRLNDLLLTLQHS

Target ORF information:

RefSeq Version	XM_010750379.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea ASH1 like histone lysine methyltransferase (ash1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010750379.2

ORF Insert Sequence:

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACG 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCA 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCGGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTGGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AAAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTGAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGT 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GATCAGCCAG TGGGGGTGGA GGTGACGGTT ATCAGCAGTG ACCCCCACCA TCAGAGTCAG 
GACACCTCCA GCTTGCTCAC TGCCAGCCCC TGTCCCACCA AAAACCAGAA TAGACAAGAG 
GAGGAGGGAG AGGAAGAAAA AAGAGGGGAG GAAGAGGTCT GCAGCAGAGA CCAGAGGAGG 
AAACGGCTGA ATGATTTACT TTTGACCCTG CAGCATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104934663 ( XM_010750379.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010748681.2 Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X1, mRNA.

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CloneID	OLa203014
Clone ID Related Accession (Same CDS sequence)	XM_010750379.3
Accession Version	XM_010750379.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040023.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010750379.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GATCAGCCAG TGGGGGTGGA GGTGACGGTT ATCAGCAGTG ACCCCCACCA TCAGAGTCAG 
GACACCTCCA GCTTGCTCAC TGCCAGCCCC TGTCCCACCA AAAACCAGAA TAGACAAGAG 
GAGGAGGGAG AGGAAGAAAA AAGAGGGGAG GAAGAGGTCT GCAGCAGAGA CCAGAGGAGG 
AAACGGCTGA ATGATTTACT TTTGACCCTG CAGCATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010748681.2
CDS	1112..7213
Translation	MDQRVKGGTPPTTNSTLEPTSAPEDSEQDQVAEKKWRGEGENRDVGEKEEEKKGAGRKREGDKGAVLELLIEGRCGSAGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGAPLRGDGEGGVGQQGKTSSAPDEATRDQRSGLDSVQTTSKQTASAASPPLASSSSLSLPSSSSSSSQPLSLLSTFTAASTPASVPAITSQGTKSAKQVPIKLADKTEVNSNGSSERVKKEKLPSVNGQPVLAGHKPCSSTTDQTASTTPSNTCIQALSASETRNEGKTSKKHNGLVQTKQKGLSNGKGSTDILSTSTSSKIKAGKHSSSSSVSSADQSPRPPVSTSTHKELSLSNKSRPDSPSSPSVTPKPQSSPNVDLSSAQSQDQDSSPQASLEKKKEKERKAKKEKRKDKKSKRDRLEAERAKSEGRKEEGKKKKKEKGKDGKSRHGKEKDERHKTDDTWRDELKVERGKGEKKRDKVSPDRQRTEDNNAKCGETVDSGKLDKTSKVVNPGRPEEIDKADDSCKPVKLSEPATGDGSKSKEQDVSRHSTVPPSHPSSSAPPSVPTPSARAPVSPPSSPQEQDSRPLKKRKARRPSWTKLVHRAQRAENQEAPSDSQHNSLLGFPQNPKTSLPAKATIQQTDESHPAPSTSLTSSSSPFSSATKPPSPKQSHPTSDSSPPASRCPITPARKRGRPKSHSSSLDEPPPRLSPNAIPTEVPLLGCDGIQKAPVLEPSPILQCAAQSKSSPKKRGRPPKRPLPEDQSGDALNRTDDADRSKDFHPPEKGNRQLKIRRLINEMKKRKKRRLQKVMLSGYVGKEGKGSEAADGETSLRMCKSIEATTVHTLSALSSSFGSKLGPQINVSKRGTIYMGKRRGRKPKTQTAPTVQTNSQNSTQSSLFSNPSETSLFSNQPQPPPSHPFPSPSLTHSSGAQSPYSEGSLAEPTSSLIFPHPFSLPSPSSSCTSPRPPSSSSLSPFVKKSCPCQGRHHFPFHQSSCKLSCPTPPLHHTPGSPGHLKEATPSPRSESHSEETLPSDSGIGTDNNSVSERGEIRGARGMLRLGQGSGVILGGQRHPSSLVDRPSPVSSPLSHMPRHTNPITNSSTVERHRERDRHRHRRRDFDCSSSCTCLCPCSCPGHNKCTHSDYYSCLGHNALKRQKNKHKKKHQQLHMQDPEFLAELEDLIGQFSEVHIGRRGWARTGLGQGFDGSGNAAGGRRHHSSSHSLRSNIFRINLNGFYSPHPSPYSANTSFSPQPFYPCQPVHCNRKPDRRQCACPSKFQETIENMGFYSSYPPATTLYHHLPSSYPLPSPHQYGPHQSHHAHFLLNPARFHRRRSRLLREGALGGEVEGDVGGGSGGGPHLSSGFTSSLSCGCGRNEHKHKHKHRHRHCERDLDDEEELHEDEEEDGMEREGLASSKPRSGFILGQGEGGRKGARGMGSMLTKESPWLCENGNDSFSSAAAVTSSASAERYKHTSLTSLGLGSSHLSSFGGSWGALGQSWTKFGGLGGTGFGNSNPSWRGFSGEQHTGRLIASDGEDDDSEDIQEPHLYRASPSPTHTNLFTSAAMATGGRGLRSGLAGKNPGSGDRSWRRDEPAWTERRETGLQGDSRSRGQQKSVPTPDSVAGKNKRRPGRPRKHPLPSTIPSPTHSSAAPSMSSPDLLPGHSHNRDGREVGGRKDERGPERDRGADTVQQVTELELQAKRKRGRKRKHGDSPCHQSVTEDKPECDTPPECFSQSDVDQAPSQPVVIQREERADGPPRKKFLRAGLYSDDYKTTDPPSQAQQLCRESMEYTPEEHEYSLLPAPIHVGKYLRLKRIHFQLPYDVMWLWQHNQLQRQPAVPMKRKRRYCRLKERTLSSHQTAEENSSDITSLFPHLDMEPLTSSERSFVVKHHVFLVRNWELVRDRQIRMRIERERERDAEAEEEDSRRLSFDRANGDDSHIKSDQPVGVEVTVISSDPHHQSQDTSSLLTASPCPTKNQNRQEEEGEEEKRGEEEVCSRDQRRKRLNDLLLTLQHS

Target ORF information:

RefSeq Version	XM_010750379.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase ASH1L (LOC104934663), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010750379.3

ORF Insert Sequence:

ATGGACCAGA GAGTGAAGGG GGGAACCCCT CCAACCACAA ACTCCACTCT GGAACCCACC 
TCTGCACCTG AAGACTCTGA ACAGGACCAA GTGGCAGAGA AAAAGTGGAG AGGTGAGGGG 
GAGAATAGAG ATGTAGGGGA AAAGGAGGAA GAGAAGAAGG GAGCAGGGAG AAAGAGAGAA 
GGAGACAAGG GGGCAGTGCT GGAGCTGCTC ATCGAGGGGC GCTGTGGAAG CGCAGGGCAG 
CAACAGCTAC AGATCTCTGG CAGAGAGACG AGCTGCCCTG AGGGGAATGT ACGACTTCGG 
ATTGGACTAC AGGCCAAACG CACCAAGAAA CCGCCCAAGA TCTTGGAGAG CTACGTCTGC 
AAGCCCACCA TCAGGACCTA TCAGAGGCAA GGCAGGGGGG CACCGCTAAG GGGGGACGGA 
GAAGGAGGAG TGGGCCAGCA GGGCAAAACC AGCTCTGCTC CAGACGAAGC AACCAGAGAT 
CAACGCTCAG GCTTGGATTC TGTCCAGACC ACATCCAAAC AGACTGCATC TGCTGCTTCA 
CCACCACTTG CATCATCATC ATCATTATCA TTGCCATCAT CATCATCTTC GTCATCACAG 
CCGCTGTCGT TATTATCAAC ATTCACTGCT GCTTCCACCC CGGCCTCAGT CCCTGCCATA 
ACCAGCCAAG GGACCAAGTC TGCCAAACAG GTTCCTATCA AACTGGCCGA TAAGACAGAG 
GTGAATTCAA ATGGCTCATC TGAGAGAGTG AAGAAAGAGA AGCTCCCTAG TGTCAATGGC 
CAGCCTGTCC TTGCAGGACA CAAACCTTGT TCGTCTACAA CAGACCAGAC AGCTTCAACA 
ACTCCTTCCA ACACATGCAT CCAAGCCCTG TCTGCATCAG AAACAAGGAA TGAAGGAAAG 
ACCTCCAAGA AACATAATGG TTTGGTACAA ACAAAACAGA AGGGACTATC GAATGGCAAA 
GGCTCTACCG ACATACTCAG CACTTCCACC TCGTCGAAAA TCAAAGCTGG AAAACACAGC 
AGTTCCTCCT CTGTTTCTTC CGCAGACCAA TCACCAAGAC CTCCAGTCTC CACCAGCACC 
CATAAAGAAC TTAGCCTGTC CAATAAGAGT AGGCCAGACT CTCCTTCTTC TCCCTCAGTC 
ACCCCTAAAC CACAGTCCTC CCCCAATGTG GATCTTTCCT CTGCCCAATC CCAAGATCAG 
GATTCTTCTC CGCAGGCCTC ACTAGAGAAA AAGAAAGAGA AAGAGAGGAA AGCAAAGAAA 
GAGAAACGAA AAGACAAAAA ATCAAAGCGA GATAGATTGG AGGCTGAAAG AGCAAAGAGT 
GAGGGCAGAA AGGAGGAAGG AAAGAAGAAG AAAAAGGAGA AAGGGAAGGA TGGAAAATCA 
AGGCATGGTA AAGAAAAGGA TGAAAGACAT AAGACTGATG ATACGTGGAG GGATGAACTA 
AAGGTTGAAA GAGGAAAGGG TGAGAAAAAG AGGGATAAAG TTAGCCCAGA CAGACAAAGA 
ACAGAGGATA ATAATGCAAA ATGTGGTGAA ACAGTTGATA GTGGTAAACT AGATAAAACC 
AGTAAAGTTG TGAATCCAGG CAGACCAGAG GAGATCGACA AGGCAGATGA CAGTTGCAAG 
CCAGTTAAAC TATCTGAGCC AGCAACAGGT GACGGCAGTA AATCAAAAGA ACAAGACGTC 
TCAAGACATT CAACTGTGCC TCCAAGCCAC CCCTCTTCTT CTGCTCCTCC CTCTGTGCCC 
ACTCCTTCTG CCCGTGCCCC TGTTTCCCCC CCTTCTTCCC CTCAAGAGCA GGATAGCCGA 
CCGCTCAAGA AACGTAAAGC CAGGCGGCCC AGCTGGACCA AGCTGGTTCA CCGAGCTCAG 
AGGGCGGAAA ATCAGGAAGC CCCGTCAGAT TCCCAACATA ATTCCTTACT AGGTTTTCCC 
CAGAACCCCA AAACGTCCCT TCCTGCCAAG GCCACTATTC AGCAGACTGA TGAGTCACAC 
CCAGCTCCCT CTACTTCCTT AACCAGCAGT TCCTCCCCTT TTTCTTCAGC AACCAAACCT 
CCATCCCCAA AGCAGAGTCA CCCCACATCA GACTCAAGCC CCCCTGCTTC CAGATGCCCC 
ATAACCCCTG CCCGGAAAAG GGGCCGCCCC AAATCCCACA GCTCTAGCTT AGACGAACCT 
CCCCCAAGAC TTTCACCAAA TGCTATCCCA ACTGAGGTGC CTCTTCTGGG CTGTGACGGA 
ATTCAGAAAG CCCCTGTGCT GGAGCCAAGT CCAATACTGC AGTGTGCTGC TCAGTCTAAA 
TCCAGCCCCA AGAAGCGTGG CCGTCCTCCC AAAAGACCCC TCCCTGAGGA CCAGAGTGGA 
GATGCACTGA ATCGCACTGA TGATGCAGAC AGGAGTAAAG ATTTTCATCC TCCTGAAAAG 
GGGAACAGGC AGCTAAAGAT CAGGAGGCTG ATTAATGAGA TGAAGAAAAG AAAGAAGAGG 
AGACTTCAAA AGGTAATGCT GTCTGGGTAT GTTGGAAAGG AGGGAAAGGG AAGCGAGGCA 
GCAGATGGTG AGACCTCTCT GAGAATGTGT AAATCGATAG AGGCTACAAC GGTACATACT 
CTCTCAGCCC TGTCCTCCTC CTTTGGGAGT AAGCTGGGCC CTCAGATCAA TGTGAGCAAA 
AGAGGGACCA TCTACATGGG CAAGAGGCGA GGACGCAAGC CTAAAACCCA AACAGCCCCC 
ACAGTCCAAA CCAACTCCCA GAACTCCACT CAGTCATCTC TGTTTAGCAA CCCCTCTGAA 
ACATCTCTCT TCTCTAACCA ACCCCAGCCT CCCCCCTCTC ACCCATTTCC CTCCCCATCT 
CTTACCCACT CCAGCGGGGC CCAGAGCCCT TATAGTGAAG GCAGCCTCGC AGAACCGACA 
TCCTCCCTAA TTTTCCCTCA CCCCTTCTCC CTCCCTTCGC CATCATCCTC CTGTACCTCC 
CCCCGTCCTC CCTCCTCCTC ATCCCTCTCT CCCTTTGTGA AGAAGAGTTG TCCATGTCAG 
GGAAGGCATC ACTTCCCCTT TCACCAGTCT TCATGTAAGC TCTCCTGTCC CACCCCACCA 
CTGCATCACA CACCTGGCTC CCCAGGCCAC CTGAAAGAGG CCACCCCCTC CCCGAGGAGC 
GAATCACACA GCGAAGAGAC ACTGCCCAGC GATAGCGGGA TTGGAACGGA TAACAACAGT 
GTTTCTGAGA GAGGGGAGAT TAGGGGAGCT CGAGGCATGC TCAGGTTAGG TCAGGGGTCA 
GGAGTGATTC TGGGGGGTCA AAGACACCCC TCCTCTCTTG TGGACCGTCC CTCTCCTGTC 
TCTTCACCCC TCTCTCACAT GCCCAGACAC ACAAACCCCA TCACCAACTC ATCAACTGTG 
GAGCGGCACA GAGAAAGAGA CCGACACAGG CACAGGCGGA GGGATTTCGA CTGTTCCTCC 
TCTTGTACTT GCTTGTGCCC GTGCTCCTGC CCAGGACACA ACAAGTGCAC TCATTCAGAT 
TATTACTCTT GCCTTGGGCA CAATGCACTG AAGAGACAGA AAAATAAACA TAAGAAGAAG 
CACCAGCAGC TGCATATGCA GGATCCAGAG TTTCTGGCTG AACTAGAAGA TCTGATCGGT 
CAGTTCAGCG AGGTCCATAT CGGACGGCGA GGTTGGGCTC GGACAGGCCT GGGACAGGGC 
TTTGATGGGA GTGGGAATGC TGCTGGAGGA AGGCGCCATC ACTCGTCCTC CCATTCTCTT 
CGCTCTAACA TCTTCAGAAT CAATCTGAAC GGCTTCTACT CACCTCACCC CTCACCCTAC 
TCTGCTAATA CCTCCTTCTC CCCTCAGCCT TTCTACCCAT GCCAGCCTGT GCATTGTAAC 
AGAAAGCCAG ACCGCAGGCA GTGTGCTTGT CCATCAAAGT TCCAAGAGAC CATTGAAAAT 
ATGGGCTTTT ACAGCAGCTA TCCTCCAGCC ACAACACTTT ACCATCACCT CCCTAGCTCC 
TACCCGCTCC CCTCTCCACA CCAGTACGGC CCCCATCAGT CGCATCATGC CCATTTCCTC 
CTCAACCCTG CCAGATTCCA CAGGCGGAGA AGCAGGTTGC TTAGGGAGGG AGCTTTAGGA 
GGAGAGGTTG AAGGAGATGT AGGAGGGGGC AGTGGAGGAG GCCCACACCT CAGCTCAGGG 
TTCACATCCA GCCTCTCCTG TGGCTGTGGG AGAAACGAAC ACAAACACAA GCATAAACAC 
CGCCACAGGC ACTGCGAGCG AGATCTGGAT GACGAGGAGG AGTTACACGA GGATGAGGAG 
GAAGATGGCA TGGAGAGAGA GGGGTTAGCC AGTTCAAAAC CAAGGTCTGG GTTTATTTTG 
GGCCAAGGAG AAGGAGGAAG GAAAGGGGCT AGAGGAATGG GGAGTATGCT GACAAAAGAA 
TCGCCTTGGT TATGTGAGAA CGGAAATGAT TCCTTCTCCT CCGCTGCTGC TGTCACATCA 
TCGGCTTCAG CAGAGAGGTA TAAACACACA TCTCTCACCT CACTGGGGCT GGGTTCTTCT 
CACTTGTCTT CGTTTGGAGG AAGCTGGGGG GCCCTGGGTC AGAGCTGGAC GAAATTCGGG 
GGCCTGGGAG GGACAGGATT TGGAAACTCA AACCCCAGCT GGAGAGGATT CAGTGGGGAG 
CAACATACAG GCCGGCTGAT TGCATCAGAC GGAGAGGACG ATGACAGCGA GGACATTCAG 
GAGCCACACC TTTACAGGGC GTCCCCGTCC CCAACACACA CCAACCTGTT CACATCTGCT 
GCCATGGCAA CAGGGGGGAG GGGTCTCAGG AGCGGATTGG CCGGTAAGAA TCCAGGAAGC 
GGAGACAGGT CATGGAGGAG AGACGAGCCA GCGTGGACAG AGAGGAGAGA AACAGGTTTA 
CAAGGTGACT CGAGAAGCCG GGGGCAGCAG AAAAGTGTGC CAACACCAGA CAGTGTGGCA 
GGGAAAAACA AAAGAAGGCC AGGTCGGCCC AGAAAGCACC CACTGCCCTC CACTATACCT 
TCCCCCACGC ACTCATCTGC AGCTCCCTCC ATGTCATCAC CTGACCTCTT GCCAGGACAC 
AGCCATAACA GAGATGGGAG AGAAGTGGGA GGGAGGAAAG ACGAAAGAGG GCCAGAGAGA 
GATCGAGGGG CCGATACAGT GCAGCAGGTG ACAGAGTTGG AGCTGCAGGC TAAGAGGAAG 
AGAGGACGGA AGAGAAAACA TGGTGACTCT CCTTGTCATC AGAGTGTCAC CGAGGATAAA 
CCCGAGTGTG ACACCCCTCC TGAATGTTTT AGCCAATCAG ATGTCGACCA GGCTCCATCC 
CAACCAGTAG TCATCCAAAG AGAGGAGAGA GCAGATGGTC CTCCCAGGAA GAAGTTTCTG 
AGGGCAGGTC TTTACTCTGA TGATTACAAA ACGACAGATC CACCCTCCCA AGCCCAGCAG 
TTGTGCAGAG AGAGTATGGA ATACACTCCA GAGGAGCATG AATACAGCCT TTTACCTGCT 
CCCATACATG TTGGGAAGTA CCTTAGGCTG AAGCGGATTC ATTTCCAATT ACCTTATGAT 
GTTATGTGGC TGTGGCAGCA CAATCAGCTT CAAAGGCAGC CTGCTGTCCC CATGAAGAGG 
AAGCGGCGTT ACTGCCGGCT GAAGGAAAGG ACTCTGTCCT CTCACCAGAC AGCGGAGGAG 
AACTCCAGTG ATATTACCAG CCTGTTTCCA CACCTCGATA TGGAGCCTTT GACCAGCAGT 
GAGAGGAGCT TTGTGGTGAA ACACCATGTT TTCCTTGTGA GGAACTGGGA GCTCGTGAGA 
GACAGACAGA TCCGAATGAG GATAGAGCGG GAGAGGGAGA GAGACGCAGA GGCAGAGGAG 
GAGGACTCAC GACGTCTGTC CTTTGATAGA GCCAATGGGG ACGACAGCCA CATCAAGTCA 
GATCAGCCAG TGGGGGTGGA GGTGACGGTT ATCAGCAGTG ACCCCCACCA TCAGAGTCAG 
GACACCTCCA GCTTGCTCAC TGCCAGCCCC TGTCCCACCA AAAACCAGAA TAGACAAGAG 
GAGGAGGGAG AGGAAGAAAA AAGAGGGGAG GAAGAGGTCT GCAGCAGAGA CCAGAGGAGG 
AAACGGCTGA ATGATTTACT TTTGACCCTG CAGCATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

LOC104934663 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム