itih2 cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus)

Gene
Clones
gRNAs

Summary

Gene Symbol	itih2
Entrez Gene ID	108233287
Full Name	inter-alpha-trypsin inhibitor heavy chain 2
Gene Type	protein-coding
Organism	Kryptolebias marmoratus(mangrove rivulus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017411657.1	XP_017267146.1	inter-alpha-trypsin inhibitor heavy chain H2
XM_017411657.2	XP_017267146.1	inter-alpha-trypsin inhibitor heavy chain H2

Pathways from BioSystems
Gene Ontology

The following itih2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itih2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OKr08493	XM_017411657.2 Latest version!	Kryptolebias marmoratus inter-alpha-trypsin inhibitor heavy chain 2 (itih2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OKr08493	XM_017411657.1	Kryptolebias marmoratus inter-alpha-trypsin inhibitor heavy chain 2 (itih2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itih2 ( XM_017411657.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017267146.1 Kryptolebias marmoratus inter-alpha-trypsin inhibitor heavy chain 2 (itih2), mRNA.

Price & Availability↑

CloneID	OKr08493
Clone ID Related Accession (Same CDS sequence)	XM_017411657.1 , XM_017411657.2
Accession Version	XM_017411657.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	inter-alpha-trypsin inhibitor heavy chain H2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCC TGCTGTTGCT GCTTCTGGGG CTCGTGATTC CCCGTCAGAG CTGCTGTTTC 
GAGTTTGTGA TCGACGGAGA ATGGGAGGAT GATGATGTGT CAGCAGCTCA GAGTCATCAA 
GAGCATCGAG AGAGGCACAA GAGAGCGACA CTGACCAGCG AGGAGCAGGA AGACTTTGGG 
GCCATCACTG GACAGGACAT CACAGTCAAG AGTTACAAGG TGGAGAGCCG CATCACGTCA 
CGCTTCGCTC ACGTCACCGT CAGGAGCTCG GTGGTCAACT CGGGCACCAA AGCTCAGAGC 
ATCGCCTTCA ACGTGCAGAT CCCCAAGCGA GCTTTCATCT CAAACTTCAC CATGAACGTG 
AATGGCATCA CCTTCGTGGG CTCGGTGAAG GAGAAGACCT TGGCCAGGAA GCTGTATGCC 
AGGGCCAGAT CGAGGAACCA CGCAGCTGGC ATCGTCAGGA CTAACTCCCA GGACCTGGAG 
GTCTTTAAGA CGGAGGTCCA CGTTCCTCCC GGCAGTAACA TCGAGTTTGA GCTTCACTAT 
CAGGAGATGA TGGAAAGGAA ACTCGGCTTC TACGAACATT CGCTGTACCT GCAGCCTGGG 
CGGCTGGTGC CACACTTCCA GGTGGATGTG TACATCTACG AGCAGAATGG AATTGCCTCT 
GTGGAAACAT CAAACACGTT TGGAACTCAG TTTGCAGAGC TCATTAAAGT TACGTCGACC 
AAAGACAAAG CTCATGTTGT CTTCAAGCCC AGCGTACAGC AGCAGAGAGA GTGTGCGAAC 
TGCACAAACA GCGCCATAGA CGGAGAGCTT ACGGTCAAAT ATGACGTAAC CAGAGACAGC 
ACCTCCGGAG AACTGCAGGT GTCCGACAGC TACTTTTTCT ATTTCTTGGC TCCTTCAAAC 
CTCTCCCCTT TTCCAAAAAA CATTGTTTTC GTTATCGATG TCAGTGGGTC CATGTGGGGC 
GTCAAAATGA AGCAGACAGT GGAAGCCATG AGAGCTATTT TAGATGACTT GACTGTCGAG 
GATCAATTCG GCATTATAGA CTTTAATCAC AACGTGCGCT GCTGGAATGA GGACCTGGTC 
CCTGGCTCCT CTCTTCACAC CACAAGTGCG AAAAGCTACA TTGAGAGCAT CAAACCCAAT 
GGAGGCACCA ACATCAACGA GGCACTAATG AGAGCGGTTC AGATGCTGAT GAGGGCGTTC 
AATCAGGACC TCATCAACTC TCGCTCCATC TCCATGATCA TTCTGGTGTC TGACGGAGAT 
CCCACTGTTG GAGAGATCAA ACTCAGCGTA ATCCAGAAGA ACATTAAGCG GGCGATGAGG 
GAGGAGTTCT CTCTCTTCTC TGTGGGCATC GGCTTCGACG TGGACTACGA CTTCCTGGAA 
CGTATCGCCA TGGAGAACAG GGGCATGGCT CAGAGGATCT ACGCCAATCA TGATGCCGCC 
GAGCAGCTAC GGACGTTTTA CCACCAGGTT TCATCTCCTC TGCTGAGTAA GATCGCCGTT 
CGCTTCAGTG ATGAATCTGT GACGAATGTC ACCCAGAGCC AGTTCGATAA GTACTTCAGC 
GGCTCGGAGC TGGTGGTGGC CGGGCGGGTT CTGCCATCAG AGAGCAGCTC ATTGAGTGGC 
TACACCTCGG CATCATCTGC CAATATGGAC CTCATTTTGG AAATGGATGC TGACATCCAC 
AAGCTGGACG AAGAGCTGGC CAAACAGCAG CACTCCTTCA CTGGATTCGC CAAACAGATG 
TGGGCTTATC TCACCATCAA ACAGCTGATC GGTGAAAGGT CTTTGGCTCC AACAGCAGCT 
AAAAAGAGGA AAATCACCCA GCGGATCATT AGCTTGTCTT TGGATCATCA GTTCGTCACG 
CCGCTCACCG CTCTGCTAGT GACAAACGAC GATGACCCCA CGGAGATGCT GCTGGCCGAC 
TCCCCGATGG ACCCCAAACA CGGCTGCTGC TCAGCTCTTC CTGCCACATC TCGTGTTACT 
TTCCCTCCCC GACCTCCTGG TGGTATGGTC TATGCAACTC CACCTTCACG TCCACACTGG 
CTCTTACCAG CCACTCAGCC ACCCACCCCA AGTCAGGTTA AAAAGGGTGA AGTGGACGAG 
ACTTTAGTGA CGACCAACGG GGTTACCAGA GTGGACGAAG ACCCCCATTT CATCATCCGA 
CTGCCTAGAA GTAACATGGA CGTCTGCTTC AACATCGACT CCAAGCCAGG TCACATCCTC 
AACCTGGTGT CAGACATTGG AACAGGTGTC GTGGTCAACG GTCAGTTAAT CAGCTCCAAA 
AAGGTTCACA AAGACAAACT AAAAACCTAC TTCGGCGTTG TCTCGGTCTA CTACCAGCCT 
GAAGGATTCA GTGTGACGGT CAGCACTGAC CGCATCACCA CGACTGATGG CAGGCGCAGC 
AACGTTTTCA CCTGGGGGTC CACCGCTGAA ATCACACACG ATGGGGTGAC GGTTTCTATT 
GTGAAGAACG CTCAAGCCAC CATCACGATA AATAACAGGA TCCAGGTAAC GGTGCTGCTT 
CACCGTGTTT GGAAGAAACA TCCAGTTAAT GTGGACTTCC TTGGGGTTTA CCTTTCCAAC 
AGCAACCAGT ATTCGCCTCT GGTCCATGGA CTCATAGGGC AGTTTTCTCA AGAGCCTGAG 
GTGAGAGTGT ACGGCGTCCA TGAAGGAGCC GACCCCCTGA AGAAGGAGGC CACCATGGAG 
GTGAAGGGCA ACAAGCTACT TGTCACCAGG GGCTGGCAGA AGGACTACAC ATGGGACAGG 
AAGCACGGCT CTAATGTCTA CTGCTGGTTC ATTCATAACA GCGGGAAGGG TTTCATCGAC 
GGTCATTACA GCGACTACAT CGTTCCAGAT CTGGACAGCT TCCTTCAGCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017267146.1
CDS	176..3052
Translation	MRCLLLLLLGLVIPRQSCCFEFVIDGEWEDDDVSAAQSHQEHRERHKRATLTSEEQEDFGAITGQDITVKSYKVESRITSRFAHVTVRSSVVNSGTKAQSIAFNVQIPKRAFISNFTMNVNGITFVGSVKEKTLARKLYARARSRNHAAGIVRTNSQDLEVFKTEVHVPPGSNIEFELHYQEMMERKLGFYEHSLYLQPGRLVPHFQVDVYIYEQNGIASVETSNTFGTQFAELIKVTSTKDKAHVVFKPSVQQQRECANCTNSAIDGELTVKYDVTRDSTSGELQVSDSYFFYFLAPSNLSPFPKNIVFVIDVSGSMWGVKMKQTVEAMRAILDDLTVEDQFGIIDFNHNVRCWNEDLVPGSSLHTTSAKSYIESIKPNGGTNINEALMRAVQMLMRAFNQDLINSRSISMIILVSDGDPTVGEIKLSVIQKNIKRAMREEFSLFSVGIGFDVDYDFLERIAMENRGMAQRIYANHDAAEQLRTFYHQVSSPLLSKIAVRFSDESVTNVTQSQFDKYFSGSELVVAGRVLPSESSSLSGYTSASSANMDLILEMDADIHKLDEELAKQQHSFTGFAKQMWAYLTIKQLIGERSLAPTAAKKRKITQRIISLSLDHQFVTPLTALLVTNDDDPTEMLLADSPMDPKHGCCSALPATSRVTFPPRPPGGMVYATPPSRPHWLLPATQPPTPSQVKKGEVDETLVTTNGVTRVDEDPHFIIRLPRSNMDVCFNIDSKPGHILNLVSDIGTGVVVNGQLISSKKVHKDKLKTYFGVVSVYYQPEGFSVTVSTDRITTTDGRRSNVFTWGSTAEITHDGVTVSIVKNAQATITINNRIQVTVLLHRVWKKHPVNVDFLGVYLSNSNQYSPLVHGLIGQFSQEPEVRVYGVHEGADPLKKEATMEVKGNKLLVTRGWQKDYTWDRKHGSNVYCWFIHNSGKGFIDGHYSDYIVPDLDSFLQLP

Target ORF information:

RefSeq Version	XM_017411657.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus inter-alpha-trypsin inhibitor heavy chain 2 (itih2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017411657.1

ORF Insert Sequence:

ATGAGGTGCC TGCTGTTGCT GCTTCTGGGG CTCGTGATTC CCCGTCAGAG CTGCTGTTTC 
GAGTTTGTGA TCGACGGAGA ATGGGAGGAT GATGATGTGT CAGCAGCTCA GAGTCATCAA 
GAGCATCGAG AGAGGCACAA GAGAGCGACA CTGACCAGCG AGGAGCAGGA AGACTTTGGG 
GCCATCACTG GACAGGACAT CACAGTCAAG AGTTACAAGG TGGAGAGCCG CATCACGTCA 
CGCTTCGCTC ACGTCACCGT CAGGAGCTCG GTGGTCAACT CGGGCACCAA AGCTCAGAGC 
ATCGCCTTCA ACGTGCAGAT CCCCAAGCGA GCTTTCATCT CAAACTTCAC CATGAACGTG 
AATGGCATCA CCTTCGTGGG CTCGGTGAAG GAGAAGACCT TGGCCAGGAA GCTGTATGCC 
AGGGCCAGAT CGAGGAACCA CGCAGCTGGC ATCGTCAGGA CTAACTCCCA GGACCTGGAG 
GTCTTTAAGA CGGAGGTCCA CGTTCCTCCC GGCAGTAACA TCGAGTTTGA GCTTCACTAT 
CAGGAGATGA TGGAAAGGAA ACTCGGCTTC TACGAACATT CGCTGTACCT GCAGCCTGGG 
CGGCTGGTGC CACACTTCCA GGTGGATGTG TACATCTACG AGCAGAATGG AATTGCCTCT 
GTGGAAACAT CAAACACGTT TGGAACTCAG TTTGCAGAGC TCATTAAAGT TACGTCGACC 
AAAGACAAAG CTCATGTTGT CTTCAAGCCC AGCGTACAGC AGCAGAGAGA GTGTGCGAAC 
TGCACAAACA GCGCCATAGA CGGAGAGCTT ACGGTCAAAT ATGACGTAAC CAGAGACAGC 
ACCTCCGGAG AACTGCAGGT GTCCGACAGC TACTTTTTCT ATTTCTTGGC TCCTTCAAAC 
CTCTCCCCTT TTCCAAAAAA CATTGTTTTC GTTATCGATG TCAGTGGGTC CATGTGGGGC 
GTCAAAATGA AGCAGACAGT GGAAGCCATG AGAGCTATTT TAGATGACTT GACTGTCGAG 
GATCAATTCG GCATTATAGA CTTTAATCAC AACGTGCGCT GCTGGAATGA GGACCTGGTC 
CCTGGCTCCT CTCTTCACAC CACAAGTGCG AAAAGCTACA TTGAGAGCAT CAAACCCAAT 
GGAGGCACCA ACATCAACGA GGCACTAATG AGAGCGGTTC AGATGCTGAT GAGGGCGTTC 
AATCAGGACC TCATCAACTC TCGCTCCATC TCCATGATCA TTCTGGTGTC TGACGGAGAT 
CCCACTGTTG GAGAGATCAA ACTCAGCGTA ATCCAGAAGA ACATTAAGCG GGCGATGAGG 
GAGGAGTTCT CTCTCTTCTC TGTGGGCATC GGCTTCGACG TGGACTACGA CTTCCTGGAA 
CGTATCGCCA TGGAGAACAG GGGCATGGCT CAGAGGATCT ACGCCAATCA TGATGCCGCC 
GAGCAGCTAC GGACGTTTTA CCACCAGGTT TCATCTCCTC TGCTGAGTAA GATCGCCGTT 
CGCTTCAGTG ATGAATCTGT GACGAATGTC ACCCAGAGCC AGTTCGATAA GTACTTCAGC 
GGCTCGGAGC TGGTGGTGGC CGGGCGGGTT CTGCCATCAG AGAGCAGCTC ATTGAGTGGC 
TACACCTCGG CATCATCTGC CAATATGGAC CTCATTTTGG AAATGGATGC TGACATCCAC 
AAGCTGGACG AAGAGCTGGC CAAACAGCAG CACTCCTTCA CTGGATTCGC CAAACAGATG 
TGGGCTTATC TCACCATCAA ACAGCTGATC GGTGAAAGGT CTTTGGCTCC AACAGCAGCT 
AAAAAGAGGA AAATCACCCA GCGGATCATT AGCTTGTCTT TGGATCATCA GTTCGTCACG 
CCGCTCACCG CTCTGCTAGT GACAAACGAC GATGACCCCA CGGAGATGCT GCTGGCCGAC 
TCCCCGATGG ACCCCAAACA CGGCTGCTGC TCAGCTCTTC CTGCCACATC TCGTGTTACT 
TTCCCTCCCC GACCTCCTGG TGGTATGGTC TATGCAACTC CACCTTCACG TCCACACTGG 
CTCTTACCAG CCACTCAGCC ACCCACCCCA AGTCAGGTTA AAAAGGGTGA AGTGGACGAG 
ACTTTAGTGA CGACCAACGG GGTTACCAGA GTGGACGAAG ACCCCCATTT CATCATCCGA 
CTGCCTAGAA GTAACATGGA CGTCTGCTTC AACATCGACT CCAAGCCAGG TCACATCCTC 
AACCTGGTGT CAGACATTGG AACAGGTGTC GTGGTCAACG GTCAGTTAAT CAGCTCCAAA 
AAGGTTCACA AAGACAAACT AAAAACCTAC TTCGGCGTTG TCTCGGTCTA CTACCAGCCT 
GAAGGATTCA GTGTGACGGT CAGCACTGAC CGCATCACCA CGACTGATGG CAGGCGCAGC 
AACGTTTTCA CCTGGGGGTC CACCGCTGAA ATCACACACG ATGGGGTGAC GGTTTCTATT 
GTGAAGAACG CTCAAGCCAC CATCACGATA AATAACAGGA TCCAGGTAAC GGTGCTGCTT 
CACCGTGTTT GGAAGAAACA TCCAGTTAAT GTGGACTTCC TTGGGGTTTA CCTTTCCAAC 
AGCAACCAGT ATTCGCCTCT GGTCCATGGA CTCATAGGGC AGTTTTCTCA AGAGCCTGAG 
GTGAGAGTGT ACGGCGTCCA TGAAGGAGCC GACCCCCTGA AGAAGGAGGC CACCATGGAG 
GTGAAGGGCA ACAAGCTACT TGTCACCAGG GGCTGGCAGA AGGACTACAC ATGGGACAGG 
AAGCACGGCT CTAATGTCTA CTGCTGGTTC ATTCATAACA GCGGGAAGGG TTTCATCGAC 
GGTCATTACA GCGACTACAT CGTTCCAGAT CTGGACAGCT TCCTTCAGCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itih2 ( XM_017411657.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017267146.1 Kryptolebias marmoratus inter-alpha-trypsin inhibitor heavy chain 2 (itih2), mRNA.

Price & Availability↑

CloneID	OKr08493
Clone ID Related Accession (Same CDS sequence)	XM_017411657.1 , XM_017411657.2
Accession Version	XM_017411657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	inter-alpha-trypsin inhibitor heavy chain H2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017411657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCC TGCTGTTGCT GCTTCTGGGG CTCGTGATTC CCCGTCAGAG CTGCTGTTTC 
GAGTTTGTGA TCGACGGAGA ATGGGAGGAT GATGATGTGT CAGCAGCTCA GAGTCATCAA 
GAGCATCGAG AGAGGCACAA GAGAGCGACA CTGACCAGCG AGGAGCAGGA AGACTTTGGG 
GCCATCACTG GACAGGACAT CACAGTCAAG AGTTACAAGG TGGAGAGCCG CATCACGTCA 
CGCTTCGCTC ACGTCACCGT CAGGAGCTCG GTGGTCAACT CGGGCACCAA AGCTCAGAGC 
ATCGCCTTCA ACGTGCAGAT CCCCAAGCGA GCTTTCATCT CAAACTTCAC CATGAACGTG 
AATGGCATCA CCTTCGTGGG CTCGGTGAAG GAGAAGACCT TGGCCAGGAA GCTGTATGCC 
AGGGCCAGAT CGAGGAACCA CGCAGCTGGC ATCGTCAGGA CTAACTCCCA GGACCTGGAG 
GTCTTTAAGA CGGAGGTCCA CGTTCCTCCC GGCAGTAACA TCGAGTTTGA GCTTCACTAT 
CAGGAGATGA TGGAAAGGAA ACTCGGCTTC TACGAACATT CGCTGTACCT GCAGCCTGGG 
CGGCTGGTGC CACACTTCCA GGTGGATGTG TACATCTACG AGCAGAATGG AATTGCCTCT 
GTGGAAACAT CAAACACGTT TGGAACTCAG TTTGCAGAGC TCATTAAAGT TACGTCGACC 
AAAGACAAAG CTCATGTTGT CTTCAAGCCC AGCGTACAGC AGCAGAGAGA GTGTGCGAAC 
TGCACAAACA GCGCCATAGA CGGAGAGCTT ACGGTCAAAT ATGACGTAAC CAGAGACAGC 
ACCTCCGGAG AACTGCAGGT GTCCGACAGC TACTTTTTCT ATTTCTTGGC TCCTTCAAAC 
CTCTCCCCTT TTCCAAAAAA CATTGTTTTC GTTATCGATG TCAGTGGGTC CATGTGGGGC 
GTCAAAATGA AGCAGACAGT GGAAGCCATG AGAGCTATTT TAGATGACTT GACTGTCGAG 
GATCAATTCG GCATTATAGA CTTTAATCAC AACGTGCGCT GCTGGAATGA GGACCTGGTC 
CCTGGCTCCT CTCTTCACAC CACAAGTGCG AAAAGCTACA TTGAGAGCAT CAAACCCAAT 
GGAGGCACCA ACATCAACGA GGCACTAATG AGAGCGGTTC AGATGCTGAT GAGGGCGTTC 
AATCAGGACC TCATCAACTC TCGCTCCATC TCCATGATCA TTCTGGTGTC TGACGGAGAT 
CCCACTGTTG GAGAGATCAA ACTCAGCGTA ATCCAGAAGA ACATTAAGCG GGCGATGAGG 
GAGGAGTTCT CTCTCTTCTC TGTGGGCATC GGCTTCGACG TGGACTACGA CTTCCTGGAA 
CGTATCGCCA TGGAGAACAG GGGCATGGCT CAGAGGATCT ACGCCAATCA TGATGCCGCC 
GAGCAGCTAC GGACGTTTTA CCACCAGGTT TCATCTCCTC TGCTGAGTAA GATCGCCGTT 
CGCTTCAGTG ATGAATCTGT GACGAATGTC ACCCAGAGCC AGTTCGATAA GTACTTCAGC 
GGCTCGGAGC TGGTGGTGGC CGGGCGGGTT CTGCCATCAG AGAGCAGCTC ATTGAGTGGC 
TACACCTCGG CATCATCTGC CAATATGGAC CTCATTTTGG AAATGGATGC TGACATCCAC 
AAGCTGGACG AAGAGCTGGC CAAACAGCAG CACTCCTTCA CTGGATTCGC CAAACAGATG 
TGGGCTTATC TCACCATCAA ACAGCTGATC GGTGAAAGGT CTTTGGCTCC AACAGCAGCT 
AAAAAGAGGA AAATCACCCA GCGGATCATT AGCTTGTCTT TGGATCATCA GTTCGTCACG 
CCGCTCACCG CTCTGCTAGT GACAAACGAC GATGACCCCA CGGAGATGCT GCTGGCCGAC 
TCCCCGATGG ACCCCAAACA CGGCTGCTGC TCAGCTCTTC CTGCCACATC TCGTGTTACT 
TTCCCTCCCC GACCTCCTGG TGGTATGGTC TATGCAACTC CACCTTCACG TCCACACTGG 
CTCTTACCAG CCACTCAGCC ACCCACCCCA AGTCAGGTTA AAAAGGGTGA AGTGGACGAG 
ACTTTAGTGA CGACCAACGG GGTTACCAGA GTGGACGAAG ACCCCCATTT CATCATCCGA 
CTGCCTAGAA GTAACATGGA CGTCTGCTTC AACATCGACT CCAAGCCAGG TCACATCCTC 
AACCTGGTGT CAGACATTGG AACAGGTGTC GTGGTCAACG GTCAGTTAAT CAGCTCCAAA 
AAGGTTCACA AAGACAAACT AAAAACCTAC TTCGGCGTTG TCTCGGTCTA CTACCAGCCT 
GAAGGATTCA GTGTGACGGT CAGCACTGAC CGCATCACCA CGACTGATGG CAGGCGCAGC 
AACGTTTTCA CCTGGGGGTC CACCGCTGAA ATCACACACG ATGGGGTGAC GGTTTCTATT 
GTGAAGAACG CTCAAGCCAC CATCACGATA AATAACAGGA TCCAGGTAAC GGTGCTGCTT 
CACCGTGTTT GGAAGAAACA TCCAGTTAAT GTGGACTTCC TTGGGGTTTA CCTTTCCAAC 
AGCAACCAGT ATTCGCCTCT GGTCCATGGA CTCATAGGGC AGTTTTCTCA AGAGCCTGAG 
GTGAGAGTGT ACGGCGTCCA TGAAGGAGCC GACCCCCTGA AGAAGGAGGC CACCATGGAG 
GTGAAGGGCA ACAAGCTACT TGTCACCAGG GGCTGGCAGA AGGACTACAC ATGGGACAGG 
AAGCACGGCT CTAATGTCTA CTGCTGGTTC ATTCATAACA GCGGGAAGGG TTTCATCGAC 
GGTCATTACA GCGACTACAT CGTTCCAGAT CTGGACAGCT TCCTTCAGCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017267146.1
CDS	186..3062
Translation	MRCLLLLLLGLVIPRQSCCFEFVIDGEWEDDDVSAAQSHQEHRERHKRATLTSEEQEDFGAITGQDITVKSYKVESRITSRFAHVTVRSSVVNSGTKAQSIAFNVQIPKRAFISNFTMNVNGITFVGSVKEKTLARKLYARARSRNHAAGIVRTNSQDLEVFKTEVHVPPGSNIEFELHYQEMMERKLGFYEHSLYLQPGRLVPHFQVDVYIYEQNGIASVETSNTFGTQFAELIKVTSTKDKAHVVFKPSVQQQRECANCTNSAIDGELTVKYDVTRDSTSGELQVSDSYFFYFLAPSNLSPFPKNIVFVIDVSGSMWGVKMKQTVEAMRAILDDLTVEDQFGIIDFNHNVRCWNEDLVPGSSLHTTSAKSYIESIKPNGGTNINEALMRAVQMLMRAFNQDLINSRSISMIILVSDGDPTVGEIKLSVIQKNIKRAMREEFSLFSVGIGFDVDYDFLERIAMENRGMAQRIYANHDAAEQLRTFYHQVSSPLLSKIAVRFSDESVTNVTQSQFDKYFSGSELVVAGRVLPSESSSLSGYTSASSANMDLILEMDADIHKLDEELAKQQHSFTGFAKQMWAYLTIKQLIGERSLAPTAAKKRKITQRIISLSLDHQFVTPLTALLVTNDDDPTEMLLADSPMDPKHGCCSALPATSRVTFPPRPPGGMVYATPPSRPHWLLPATQPPTPSQVKKGEVDETLVTTNGVTRVDEDPHFIIRLPRSNMDVCFNIDSKPGHILNLVSDIGTGVVVNGQLISSKKVHKDKLKTYFGVVSVYYQPEGFSVTVSTDRITTTDGRRSNVFTWGSTAEITHDGVTVSIVKNAQATITINNRIQVTVLLHRVWKKHPVNVDFLGVYLSNSNQYSPLVHGLIGQFSQEPEVRVYGVHEGADPLKKEATMEVKGNKLLVTRGWQKDYTWDRKHGSNVYCWFIHNSGKGFIDGHYSDYIVPDLDSFLQLP

Target ORF information:

RefSeq Version	XM_017411657.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus inter-alpha-trypsin inhibitor heavy chain 2 (itih2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017411657.2

ORF Insert Sequence:

ATGAGGTGCC TGCTGTTGCT GCTTCTGGGG CTCGTGATTC CCCGTCAGAG CTGCTGTTTC 
GAGTTTGTGA TCGACGGAGA ATGGGAGGAT GATGATGTGT CAGCAGCTCA GAGTCATCAA 
GAGCATCGAG AGAGGCACAA GAGAGCGACA CTGACCAGCG AGGAGCAGGA AGACTTTGGG 
GCCATCACTG GACAGGACAT CACAGTCAAG AGTTACAAGG TGGAGAGCCG CATCACGTCA 
CGCTTCGCTC ACGTCACCGT CAGGAGCTCG GTGGTCAACT CGGGCACCAA AGCTCAGAGC 
ATCGCCTTCA ACGTGCAGAT CCCCAAGCGA GCTTTCATCT CAAACTTCAC CATGAACGTG 
AATGGCATCA CCTTCGTGGG CTCGGTGAAG GAGAAGACCT TGGCCAGGAA GCTGTATGCC 
AGGGCCAGAT CGAGGAACCA CGCAGCTGGC ATCGTCAGGA CTAACTCCCA GGACCTGGAG 
GTCTTTAAGA CGGAGGTCCA CGTTCCTCCC GGCAGTAACA TCGAGTTTGA GCTTCACTAT 
CAGGAGATGA TGGAAAGGAA ACTCGGCTTC TACGAACATT CGCTGTACCT GCAGCCTGGG 
CGGCTGGTGC CACACTTCCA GGTGGATGTG TACATCTACG AGCAGAATGG AATTGCCTCT 
GTGGAAACAT CAAACACGTT TGGAACTCAG TTTGCAGAGC TCATTAAAGT TACGTCGACC 
AAAGACAAAG CTCATGTTGT CTTCAAGCCC AGCGTACAGC AGCAGAGAGA GTGTGCGAAC 
TGCACAAACA GCGCCATAGA CGGAGAGCTT ACGGTCAAAT ATGACGTAAC CAGAGACAGC 
ACCTCCGGAG AACTGCAGGT GTCCGACAGC TACTTTTTCT ATTTCTTGGC TCCTTCAAAC 
CTCTCCCCTT TTCCAAAAAA CATTGTTTTC GTTATCGATG TCAGTGGGTC CATGTGGGGC 
GTCAAAATGA AGCAGACAGT GGAAGCCATG AGAGCTATTT TAGATGACTT GACTGTCGAG 
GATCAATTCG GCATTATAGA CTTTAATCAC AACGTGCGCT GCTGGAATGA GGACCTGGTC 
CCTGGCTCCT CTCTTCACAC CACAAGTGCG AAAAGCTACA TTGAGAGCAT CAAACCCAAT 
GGAGGCACCA ACATCAACGA GGCACTAATG AGAGCGGTTC AGATGCTGAT GAGGGCGTTC 
AATCAGGACC TCATCAACTC TCGCTCCATC TCCATGATCA TTCTGGTGTC TGACGGAGAT 
CCCACTGTTG GAGAGATCAA ACTCAGCGTA ATCCAGAAGA ACATTAAGCG GGCGATGAGG 
GAGGAGTTCT CTCTCTTCTC TGTGGGCATC GGCTTCGACG TGGACTACGA CTTCCTGGAA 
CGTATCGCCA TGGAGAACAG GGGCATGGCT CAGAGGATCT ACGCCAATCA TGATGCCGCC 
GAGCAGCTAC GGACGTTTTA CCACCAGGTT TCATCTCCTC TGCTGAGTAA GATCGCCGTT 
CGCTTCAGTG ATGAATCTGT GACGAATGTC ACCCAGAGCC AGTTCGATAA GTACTTCAGC 
GGCTCGGAGC TGGTGGTGGC CGGGCGGGTT CTGCCATCAG AGAGCAGCTC ATTGAGTGGC 
TACACCTCGG CATCATCTGC CAATATGGAC CTCATTTTGG AAATGGATGC TGACATCCAC 
AAGCTGGACG AAGAGCTGGC CAAACAGCAG CACTCCTTCA CTGGATTCGC CAAACAGATG 
TGGGCTTATC TCACCATCAA ACAGCTGATC GGTGAAAGGT CTTTGGCTCC AACAGCAGCT 
AAAAAGAGGA AAATCACCCA GCGGATCATT AGCTTGTCTT TGGATCATCA GTTCGTCACG 
CCGCTCACCG CTCTGCTAGT GACAAACGAC GATGACCCCA CGGAGATGCT GCTGGCCGAC 
TCCCCGATGG ACCCCAAACA CGGCTGCTGC TCAGCTCTTC CTGCCACATC TCGTGTTACT 
TTCCCTCCCC GACCTCCTGG TGGTATGGTC TATGCAACTC CACCTTCACG TCCACACTGG 
CTCTTACCAG CCACTCAGCC ACCCACCCCA AGTCAGGTTA AAAAGGGTGA AGTGGACGAG 
ACTTTAGTGA CGACCAACGG GGTTACCAGA GTGGACGAAG ACCCCCATTT CATCATCCGA 
CTGCCTAGAA GTAACATGGA CGTCTGCTTC AACATCGACT CCAAGCCAGG TCACATCCTC 
AACCTGGTGT CAGACATTGG AACAGGTGTC GTGGTCAACG GTCAGTTAAT CAGCTCCAAA 
AAGGTTCACA AAGACAAACT AAAAACCTAC TTCGGCGTTG TCTCGGTCTA CTACCAGCCT 
GAAGGATTCA GTGTGACGGT CAGCACTGAC CGCATCACCA CGACTGATGG CAGGCGCAGC 
AACGTTTTCA CCTGGGGGTC CACCGCTGAA ATCACACACG ATGGGGTGAC GGTTTCTATT 
GTGAAGAACG CTCAAGCCAC CATCACGATA AATAACAGGA TCCAGGTAAC GGTGCTGCTT 
CACCGTGTTT GGAAGAAACA TCCAGTTAAT GTGGACTTCC TTGGGGTTTA CCTTTCCAAC 
AGCAACCAGT ATTCGCCTCT GGTCCATGGA CTCATAGGGC AGTTTTCTCA AGAGCCTGAG 
GTGAGAGTGT ACGGCGTCCA TGAAGGAGCC GACCCCCTGA AGAAGGAGGC CACCATGGAG 
GTGAAGGGCA ACAAGCTACT TGTCACCAGG GGCTGGCAGA AGGACTACAC ATGGGACAGG 
AAGCACGGCT CTAATGTCTA CTGCTGGTTC ATTCATAACA GCGGGAAGGG TTTCATCGAC 
GGTCATTACA GCGACTACAT CGTTCCAGAT CTGGACAGCT TCCTTCAGCT GCCCTGA